TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161284.2.6
(621 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512939.1|CO512939 s13dSG86F0300027_121804 Glandular tr... 172 4e-044
gb|CO515516.1|CO515516 s13dSG52E1200099_418091 Glandular tr... 168 7e-043
emb|X63376.1|MSC29 M.sativa C29 mRNA for snRNP-related protein 156 2e-039
>gb|CO512939.1|CO512939 s13dSG86F0300027_121804 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 566
Score = 172 bits (370), Expect = 4e-044
Identities = 72/86 (83%), Positives = 80/86 (93%)
Frame = -3 / +2
Query: 484 TMSRSGQPPDLKKYMDKKLQIKMNANRVVIGTLRGFDQFMNLVVDNTVEVNGNDKTDIGM 305
TMSRSGQPPDLKKYMDK+LQIK+NANR+++GTLRGFDQFMNLVVDNTVEVNGN+K DIGM
Sbjct: 80 TMSRSGQPPDLKKYMDKQLQIKLNANRMIVGTLRGFDQFMNLVVDNTVEVNGNEKNDIGM 259
Query: 304 VVIRGNSVVMIEALEPVARSQ*IIVL 227
VVIRGNSVV +EALEPV RS * + L
Sbjct: 260 VVIRGNSVVTVEALEPVNRSI*FLFL 337
Score = 103 bits (220), Expect = 2e-023
Identities = 47/79 (59%), Positives = 51/79 (64%)
Frame = +3 / -3
Query: 249 LATGSSASXXXXXXXXXXXXXXSVLSFPLTSTVLSTTRFMNWSNPRSVPITTRFAFILI* 428
L TGSSAS S S P TSTVLSTT+F+NWS PR VP RFAF LI*
Sbjct: 315 LFTGSSASTVTTLFPLITTIPMSFFSLPFTSTVLSTTKFINWSKPRRVPTIMRFAFSLI* 136
Query: 429 SFLSMYFLRSGGCPERLMV 485
S LSMYF +SGGCP+ LMV
Sbjct: 135 SCLSMYFFKSGGCPDLLMV 79
>gb|CO515516.1|CO515516 s13dSG52E1200099_418091 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 473
Score = 168 bits (361), Expect = 7e-043
Identities = 68/78 (87%), Positives = 76/78 (97%)
Frame = -3 / +1
Query: 487 VTMSRSGQPPDLKKYMDKKLQIKMNANRVVIGTLRGFDQFMNLVVDNTVEVNGNDKTDIG 308
+TMSRSGQPPDLKKYMDK+LQIK+NANR+++GTLRGFDQFMNLVVDNTVEVNGN+K DIG
Sbjct: 34 LTMSRSGQPPDLKKYMDKQLQIKLNANRMIVGTLRGFDQFMNLVVDNTVEVNGNEKNDIG 213
Query: 307 MVVIRGNSVVMIEALEPV 254
MVVIRGNSVV +EALEPV
Sbjct: 214 MVVIRGNSVVTVEALEPV 267
Score = 102 bits (218), Expect = 4e-023
Identities = 46/77 (59%), Positives = 50/77 (64%)
Frame = +3 / -1
Query: 255 TGSSASXXXXXXXXXXXXXXSVLSFPLTSTVLSTTRFMNWSNPRSVPITTRFAFILI*SF 434
TGSSAS S S P TSTVLSTT+F+NWS PR VP RFAF LI*S
Sbjct: 266 TGSSASTVTTLFPLITTIPMSFFSLPFTSTVLSTTKFINWSKPRRVPTIIRFAFSLI*SC 87
Query: 435 LSMYFLRSGGCPERLMV 485
LSMYF +SGGCP+ LMV
Sbjct: 86 LSMYFFKSGGCPDLLMV 36
>emb|X63376.1|MSC29 M.sativa C29 mRNA for snRNP-related protein
Length = 376
Score = 156 bits (336), Expect = 2e-039
Identities = 64/78 (82%), Positives = 72/78 (92%)
Frame = -3 / +1
Query: 487 VTMSRSGQPPDLKKYMDKKLQIKMNANRVVIGTLRGFDQFMNLVVDNTVEVNGNDKTDIG 308
+TMS SGQPP LKKYMDK+LQI + ANR+++GTLRGFDQFMNLVVDNTVEVNGN+K DIG
Sbjct: 4 LTMSTSGQPPALKKYMDKQLQINLKANRMIVGTLRGFDQFMNLVVDNTVEVNGNEKNDIG 183
Query: 307 MVVIRGNSVVMIEALEPV 254
MVVIRGNSVV +EALEPV
Sbjct: 184 MVVIRGNSVVTVEALEPV 237
Score = 100 bits (213), Expect = 2e-022
Identities = 45/77 (58%), Positives = 50/77 (64%)
Frame = +3 / -3
Query: 255 TGSSASXXXXXXXXXXXXXXSVLSFPLTSTVLSTTRFMNWSNPRSVPITTRFAFILI*SF 434
TGSSAS S S P TSTVLSTT+F+NWS PR VP RFAF LI*S
Sbjct: 236 TGSSASTVTTLFPLITTIPMSFFSLPFTSTVLSTTKFINWSKPRRVPTIIRFAFRLI*SC 57
Query: 435 LSMYFLRSGGCPERLMV 485
LSMYF ++GGCP+ LMV
Sbjct: 56 LSMYFFKAGGCPDVLMV 6
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,112,628
Number of Sequences: 7669
Number of extensions: 67958
Number of successful extensions: 1097
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 154132935
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)