TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161272.2.28
(3878 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular tr... 332 1e-091
gb|DQ122795.1| Medicago sativa clone M22 putative cellulose... 129 7e-048
>gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 529
Score = 332 bits (720), Expect = 1e-091
Identities = 128/174 (73%), Positives = 144/174 (82%)
Frame = -1 / +1
Query: 1433 ALGSIEIFFSNHCPLWYGYGGGLKFLERFSYINSIVYPWTSIPLLAYCTLPAICLLTGKF 1254
ALGS+EIFFS HCP+WYGYGGGLK LERFSYINS+VYPWTS+PL+ YCTLPAICLLTGKF
Sbjct: 7 ALGSVEIFFSKHCPIWYGYGGGLKLLERFSYINSVVYPWTSLPLIVYCTLPAICLLTGKF 186
Query: 1253 ITPELNNVASLWFMSLFICIFATSILEMRWSGVGIDDWWRNEQFWVIGGVSSHLFAVFQG 1074
I PE++N ASL FM+LFI I AT ILEM+W GVGIDDWWRNEQFWVIGG SSH FA+FQG
Sbjct: 187 IVPEISNYASLVFMALFISIAATGILEMQWGGVGIDDWWRNEQFWVIGGASSHFFALFQG 366
Query: 1073 LLKVIAGVDTSFTVTSKGGDDEEFSELYTFKWXXXXXXXXXXXXLNFIGVVAGI 912
LLKV+AGVDT+FTVTSK DD EFSELY FKW +N +GV+ G+
Sbjct: 367 LLKVLAGVDTNFTVTSKAADDGEFSELYIFKWTSLLIPPMTLLIMNIVGVIVGV 528
Score = 217 bits (469), Expect = 6e-057
Identities = 98/171 (57%), Positives = 109/171 (63%)
Frame = +1 / -3
Query: 916 PATTPMKFXXXXXXXXXXXXXHLNVYXXXXXXXXPPLDVTVKLVSTPAMTLSSPWNTAKR 1095
P TP F HLN+ DVTVK VSTPA TLS PW AK+
Sbjct: 524 PTITPTIFMINSVIGGINSEVHLNM*SSENSPSSAAFDVTVKFVSTPAKTLSKPWKRAKK 345
Query: 1096 CEDTPPMTQNCSFLHQSSMPTPLHLISRMLVAKMQMKSDMNHRLATLLSSGVINFPVSKQ 1275
C++ PP+TQNCSFLHQSS+PTP H ISR+ VA M MK MN RLA LL SG INFPV +
Sbjct: 344 CDEAPPITQNCSFLHQSSIPTPPHCISRIPVAAMDMKRAMNTRLA*LLISGTINFPVRRH 165
Query: 1276 MAGNVQ*AKSGIDVQGYTMELMYENLSRNFSPPPYPYQRGQWLLKKISIEP 1428
MAG VQ* +G +VQGYT E M E S NF+PPP PYQ GQ LLKKIS EP
Sbjct: 164 MAGRVQ*TINGREVQGYTTEFM*EKRSNNFNPPP*PYQIGQCLLKKISTEP 12
Score = 151 bits (324), Expect = 6e-037
Identities = 70/171 (40%), Positives = 94/171 (54%)
Frame = -3 / +3
Query: 1428 WFN*NFLQQPLPSLVWVWWWTKVPGKVFVH*LHRIPLDIYPALGLLHIACHLLADREIYH 1249
WF *+ QQ L +LV + WW +V G F+H R+ LD P LL+ H+ +DR+IY
Sbjct: 12 WFC*DLFQQTLSNLVRLRWWVEVVGTFFLHKFSRVSLDFPPIDCLLYSTGHMSSDRKIYC 191
Query: 1248 ARA*QCCQPVVHVTFHLHFCYEHPGNEMEWCRHR*LVEKRAVLGHWRRVFTSLCCVPGTT 1069
+ Q C+ VH H+H +P + M WC +R*LVE+ VLG WR T C P
Sbjct: 192 S*DKQLCKSCVHGPLHIHRSNRYP*DAMGWCWNR*LVEE*TVLGDWRCFITFFCPFPRFA 371
Query: 1068 QGHSWCRHELHCDIQGRRRRGVLRAVHIQMDDPSDTSDNPAPTELHWSGSW 916
QG SWCRH+LHC I+ R +L A+HIQMD + S++ E W
Sbjct: 372 QGFSWCRHKLHCYIKSSR*WRILGALHIQMDFAINPSNDAINHEYCGRDCW 524
Score = 122 bits (262), Expect = 2e-028
Identities = 60/154 (38%), Positives = 89/154 (57%)
Frame = +3 / -2
Query: 978 PFECVQL*ELLVVSALGCHSEARVYTSYDLE*SLEHSKEM*RHASNDPKLLVSPPVIDAY 1157
PFE V+L E ++ C+SE V TS +LE +LE K+M* SN PKL + PP+I +
Sbjct: 462 PFEYVELREFSIIGCF*CNSEVCVDTS*NLEQTLEKGKKM**STSNHPKLFIPPPIIYSN 283
Query: 1158 TTPSHFQDARSKNADEK*HEPQAGNIVKLWRDKFPCQQADGRQCAIGQERDRCPRVYDGV 1337
TTP H +D + E+ HE + + L +KF Q+ GR + + + PR++D +
Sbjct: 282 TTPLHLKDTGCCDGYEEGHEHKTCIVAYLRNNKFSGQKTYGR*STVDNQWEGSPRIHD*I 103
Query: 1338 NVRKPFQEL*STTIPIPERAVVAEENFN*TKSPP 1439
V K FQ+L TT+ +P + AE++ N TKSPP
Sbjct: 102 YVGKTFQQLQPTTVAVPNWTMFAEKDLNRTKSPP 1
>gb|DQ122795.1| Medicago sativa clone M22 putative cellulose synthase catalytic
subunit mRNA, partial cds
Length = 512
Score = 129 bits (276), Expect(2) = 7e-048
Identities = 49/97 (50%), Positives = 69/97 (71%)
Frame = -1 / +2
Query: 1295 YCTLPAICLLTGKFITPELNNVASLWFMSLFICIFATSILEMRWSGVGIDDWWRNEQFWV 1116
YC LPA+ L +G+FI L+ ++ + + + + ++LE++WSG+ + DWWRNEQFW+
Sbjct: 2 YCFLPALSLFSGQFIVQSLSVTFLVFLLGITVTLCLLALLEIKWSGITLHDWWRNEQFWL 181
Query: 1115 IGGVSSHLFAVFQGLLKVIAGVDTSFTVTSKGGDDEE 1005
IGG S+H AV QGLLKVIAGVD SFT+TSK E+
Sbjct: 182 IGGTSAHPAAVLQGLLKVIAGVDISFTLTSKSATPED 292
Score = 78.5 bits (165), Expect(2) = 7e-048
Identities = 28/72 (38%), Positives = 42/72 (58%)
Frame = -1 / +2
Query: 1013 DEEFSELYTFKWXXXXXXXXXXXXLNFIGVVAGISNAINNGYESWGPLFGKLFFAFWVIV 834
++EF++LY KW +N I + G++ + + + W L G LFF+FWV+
Sbjct: 296 EDEFADLYLVKWSFLMVPPITIMMVNTIAIAVGVARTLYSPFPQWSRLVGGLFFSFWVLC 475
Query: 833 HLYPFLKGLVGR 798
HLYPF KGL+GR
Sbjct: 476 HLYPFAKGLLGR 511
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 33,931,951
Number of Sequences: 7669
Number of extensions: 500835
Number of successful extensions: 2514
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 46
effective length of database: 895,794
effective search space used: 1116159324
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)