TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161272.2.21
(693 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515781.1|CO515781 s13dSG77H0100016_419711 Glandular tr... 118 3e-044
gb|DR159680.1|DR159680 EST34 Medicago sativa cDNA-AFLP Medi... 105 4e-024
>gb|CO515781.1|CO515781 s13dSG77H0100016_419711 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 550
Score = 118 bits (252), Expect(3) = 3e-044
Identities = 48/88 (54%), Positives = 59/88 (67%)
Frame = +3 / +2
Query: 3 YKRLINLLKENGIEPYVTLFHWDTPQALVDSYGGFLDDRIVKDYTDFAKVCFVHFGDVVK 182
Y LIN L NGI+P+VTL ++D PQAL D Y G+L ++KD+T++A VCF FGD VK
Sbjct: 11 YNNLINELIRNGIQPHVTLHNYDLPQALEDEYEGWLSREVIKDFTNYADVCFREFGDRVK 190
Query: 183 NWFTFNEPQTFSSFSYGTGIFAPGRCSP 266
W T NEP F+ SY GI P RCSP
Sbjct: 191 YWTTVNEPNIFAVGSYDQGISPPRRCSP 274
Score = 46.4 bits (95), Expect(3) = 3e-044
Identities = 19/39 (48%), Positives = 24/39 (61%)
Frame = +3 / +3
Query: 420 MGRVPYDNMFLDDQAQERSIDYNLGWFMEPVVRGDYPFS 536
+G VP N D A +R D+ LGW MEP++ GDYP S
Sbjct: 432 LGVVPQTNTEKDRAACQRISDFYLGWIMEPLLHGDYPDS 548
Score = 46.4 bits (95), Expect(3) = 3e-044
Identities = 18/45 (40%), Positives = 27/45 (60%)
Frame = +3 / +2
Query: 291 GNSLTEPYIVAHNLLRAHAETVHEYNKHYRGNKDAQIGIEKNVMG 425
GNS EPY+V H++L AH+ V Y + YR ++ +GI +G
Sbjct: 302 GNSTFEPYLVVHHILLAHSSAVRLYRRTYREEQNGFVGISIYXIG 436
>gb|DR159680.1|DR159680 EST34 Medicago sativa cDNA-AFLP Medicago sativa cDNA clone S15 5',
mRNA sequence
Length = 352
Score = 105 bits (225), Expect = 4e-024
Identities = 45/97 (46%), Positives = 59/97 (60%)
Frame = +3 / +3
Query: 117 RIVKDYTDFAKVCFVHFGDVVKNWFTFNEPQTFSSFSYGTGIFAPGRCSPGQKCANPTGN 296
RIV D+ D+A++CF FGD VK+W T NEP + S +Y G FAPGRCS G+
Sbjct: 21 RIVDDFRDYAELCFKEFGDRVKHWITLNEPWSVSMNAYAYGKFAPGRCSDWLNLNCTGGD 200
Query: 297 SLTEPYIVAHNLLRAHAETVHEYNKHYRGNKDAQIGI 407
S EPY+ AH L AHA V Y Y+ +++ +IGI
Sbjct: 201 SGAEPYLAAHYQLLAHAAAVKLYRTKYQASQNGKIGI 311
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,398,452
Number of Sequences: 7669
Number of extensions: 90577
Number of successful extensions: 1371
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 174176360
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)