TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161072.2.1
(1197 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|X58180.1|MSPAL M.sativa PAL mRNA for phenylalanine ammo... 171 5e-096
gb|CO516771.1|CO516771 s13dSG94C0200006_446870 Glandular tr... 124 6e-042
>emb|X58180.1|MSPAL M.sativa PAL mRNA for phenylalanine ammonia-lyase
Length = 2409
Score = 171 bits (369), Expect(5) = 5e-096
Identities = 71/111 (63%), Positives = 91/111 (81%)
Frame = -1 / +1
Query: 1005 RKTAEAIDILKLMSSTYIVALCQAVDLRHLEENIKASVKNTVTQVAKKVLTMNPSGELSS 826
RKT EAI+IL+LMSST+++ALCQA+DLRHLEEN+K SVKNTV+QVAKK LTM +GEL
Sbjct: 1618 RKTNEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNTVSQVAKKTLTMGVNGELHP 1797
Query: 825 ARFSEKELISAIDREAVFTYAEDAASGSLPLMQKVRAVLVDHALSSGDAER 673
+RF EK+L+ +DRE VF Y +D S + PL QK+R VLVDHAL +G++E+
Sbjct: 1798 SRFCEKDLLKVVDREHVFAYIDDPCSATYPLSQKLRQVLVDHALVNGESEK 1950
Score = 138 bits (297), Expect(5) = 5e-096
Identities = 54/102 (52%), Positives = 70/102 (68%)
Frame = -1 / +1
Query: 666 SVFSKITRFEEELRAVLPQXXXXXXXXXXEGTAPVANRIADSRSFPLYRFVREELGCVFL 487
S+F KI FEEEL+ +LP+ G + N+I RS+PLY+FVREELG L
Sbjct: 1963 SIFQKIATFEEELKTLLPKEVESARTAYESGNPTIPNKINGCRSYPLYKFVREELGTGLL 2142
Query: 486 TGERLKSPGEECNKVFVGISQGKLVDPMLECLKEWDGKPLPI 361
TGE + SPGEEC+K+F + QGK++DP+LECL EW+G PLPI
Sbjct: 2143 TGENVISPGEECDKLFSAMCQGKIIDPLLECLGEWNGAPLPI 2268
Score = 91.8 bits (194), Expect(5) = 4e-044
Identities = 52/110 (47%), Positives = 64/110 (58%)
Frame = +2 / -2
Query: 677 SASPLLRAWSTSTARTFCISGRLPLAASSAYVNTASRSMALISSFSLKRALESSPEGFMV 856
S SP A ST+T +F ++G + L SS NT S S SFS SSP MV
Sbjct: 1946 SLSPFTSA*STNTCLSF*LNGYVALQGSSI*ANTCSLSTTFNKSFSQNLEG*SSPLTPMV 1767
Query: 857 STFLATWVTVFFTDALMFSSRWRRSTAWHSATM*VDDMSFRMSIASAVFL 1006
FLAT +TVF T+ FSS+ +S AWHSA V+DMS R+S+AS VFL
Sbjct: 1766 RVFLAT*LTVFLTEFFKFSSKCLKSIAWHSAIKKVEDMS*RISMASFVFL 1617
Score = 61.1 bits (127), Expect(5) = 4e-044
Identities = 31/71 (43%), Positives = 37/71 (52%)
Frame = +2 / -2
Query: 365 GSGLPSHSLRHSSMGSTSLPWLMPTNTLLHSSPGDLSLSPVRNTQPSSSRTKRYSGNDRL 544
G G P HS +HS GS P + N+L HSSPG+++ SPV PSSS T SG D
Sbjct: 2264 GRGAPFHSPKHSRRGSMIFP*HIAENSLSHSSPGEMTFSPVSKPVPSSSLTNL*SG*DLH 2085
Query: 545 SAIRFATGAVP 577
I F P
Sbjct: 2084 PLILFGIVGFP 2052
Score = 60.2 bits (125), Expect(5) = 5e-096
Identities = 24/42 (57%), Positives = 28/42 (66%)
Frame = -1 / +1
Query: 1197 NNGLTSNLAGXATPAWTTASRGTEIAMASYCSELQYXGTPIT 1072
NNGL SNL+ P+ +G EIAMASYCSELQY P+T
Sbjct: 1429 NNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT 1554
Score = 41.8 bits (85), Expect(5) = 4e-044
Identities = 21/42 (50%), Positives = 26/42 (61%)
Frame = +2 / -2
Query: 1070 LVMGVPXYWSSEQ*EAMAISVPLEAVVQAGVAXPARFEVSPL 1195
+V G Y +SEQ*EAMAIS PL+ + G RFE +PL
Sbjct: 1556 VVTGFARYCNSEQ*EAMAISAPLKP*SKLGFLLAERFEGNPL 1431
Score = 29.5 bits (58), Expect(5) = 5e-096
Identities = 11/14 (78%), Positives = 14/14 (100%)
Frame = -3 / +1
Query: 1042 NQDVNSLGLVSAQE 1001
NQDVNSLGL+SA++
Sbjct: 1582 NQDVNSLGLISARK 1623
Score = 26.3 bits (51), Expect(5) = 4e-044
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +1 / -2
Query: 1045 CCSALCTWVGDGGA 1086
CCSAL TWV G A
Sbjct: 1580 CCSALWTWVVTGFA 1539
Score = 25.8 bits (50), Expect(5) = 4e-044
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +3 / -2
Query: 1008 AETRPREFTSW 1040
AE +P+EFTSW
Sbjct: 1616 AEIKPKEFTSW 1584
Score = 22.6 bits (43), Expect(5) = 5e-096
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -2 / +1
Query: 1067 HVQSAEQH 1044
HVQSAEQH
Sbjct: 1558 HVQSAEQH 1581
>gb|CO516771.1|CO516771 s13dSG94C0200006_446870 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 404
Score = 124 bits (265), Expect(3) = 6e-042
Identities = 50/95 (52%), Positives = 71/95 (74%)
Frame = -1 / +3
Query: 948 ALCQAVDLRHLEENIKASVKNTVTQVAKKVLTMNPSGELSSARFSEKELISAIDREAVFT 769
++ + DLRH+EEN+K+ VKNTV+QVAK++LT+ +GEL +RF EK+L++ ++ E VF
Sbjct: 96 SIVPSFDLRHIEENLKSVVKNTVSQVAKRILTVGVNGELHPSRFCEKDLLNVVEGEYVFA 275
Query: 768 YAEDAASGSLPLMQKVRAVLVDHALSSGDAEREPS 664
Y +D S + PLMQK+R VLVDHAL +GD E S
Sbjct: 276 YVDDPCSATYPLMQKLRHVLVDHALQNGDKEANSS 380
Score = 50.6 bits (104), Expect(3) = 6e-042
Identities = 20/25 (80%), Positives = 25/25 (100%)
Frame = -1 / +1
Query: 1005 RKTAEAIDILKLMSSTYIVALCQAV 931
RKTAEA++ILKLMSST++VALCQA+
Sbjct: 40 RKTAEAVEILKLMSSTFLVALCQAL 114
Score = 29.5 bits (58), Expect(3) = 6e-042
Identities = 11/14 (78%), Positives = 14/14 (100%)
Frame = -3 / +1
Query: 1042 NQDVNSLGLVSAQE 1001
NQDVNSLGL+SA++
Sbjct: 4 NQDVNSLGLISARK 45
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,106,945
Number of Sequences: 7669
Number of extensions: 83614
Number of successful extensions: 1103
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 326174355
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)