TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804944.2.2
(4065 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular tr... 333 1e-091
gb|DQ122795.1| Medicago sativa clone M22 putative cellulose... 129 6e-048
>gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 529
Score = 333 bits (721), Expect = 1e-091
Identities = 129/174 (74%), Positives = 144/174 (82%)
Frame = -2 / +1
Query: 1436 ALGSIEIFFSNHCPLWYGYGGGLKFLERFSYINSIVYPWTSIPLLAYCTLPAICLLTGKF 1257
ALGS+EIFFS HCP+WYGYGGGLK LERFSYINS+VYPWTS+PL+ YCTLPAICLLTGKF
Sbjct: 7 ALGSVEIFFSKHCPIWYGYGGGLKLLERFSYINSVVYPWTSLPLIVYCTLPAICLLTGKF 186
Query: 1256 ITPELNNVASLWFMSLFICIFATSILEMRWSGVGIDDWWRNEQFWVIGGVSSHLFAVFQG 1077
I PE++N ASL FM+LFI I AT ILEM+W GVGIDDWWRNEQFWVIGG SSH FA+FQG
Sbjct: 187 IVPEISNYASLVFMALFISIAATGILEMQWGGVGIDDWWRNEQFWVIGGASSHFFALFQG 366
Query: 1076 LLKVIAGVDTSFTVTSKGGDDEEFSELYTFKWXXXXXXXXXXXXLNFIGVVAGV 915
LLKV+AGVDT+FTVTSK DD EFSELY FKW +N +GV+ GV
Sbjct: 367 LLKVLAGVDTNFTVTSKAADDGEFSELYIFKWTSLLIPPMTLLIMNIVGVIVGV 528
Score = 219 bits (472), Expect = 2e-057
Identities = 101/174 (58%), Positives = 113/174 (64%)
Frame = +1 / -3
Query: 916 TPATTPMKFNRSKVVGGINKVVHLNVYXXXXXXXXPPFDVTVKLVSTPAMTLRSPWNTAK 1095
TP TP F + V+GGIN VHLN+ FDVTVK VSTPA TL PW AK
Sbjct: 527 TPTITPTIFMINSVIGGINSEVHLNM*SSENSPSSAAFDVTVKFVSTPAKTLSKPWKRAK 348
Query: 1096 RCEDTPPMTQNCSFLHQSSIPTPLHLISRMLVAKMQIKSDMNHRLATLFSSGVINFPVNK 1275
+C++ PP+TQNCSFLHQSSIPTP H ISR+ VA M +K MN RLA L SG INFPV +
Sbjct: 347 KCDEAPPITQNCSFLHQSSIPTPPHCISRIPVAAMDMKRAMNTRLA*LLISGTINFPVRR 168
Query: 1276 QMAGNVQ*AKRGIDVQGYTMELM*ENLSKNFRXXXXXXQRGQ*LLKKISIDPRA 1437
MAG VQ* G +VQGYT E M*E S NF Q GQ LLKKIS +PRA
Sbjct: 167 HMAGRVQ*TINGREVQGYTTEFM*EKRSNNFNPPP*PYQIGQCLLKKISTEPRA 6
Score = 161 bits (346), Expect = 6e-040
Identities = 76/159 (47%), Positives = 97/159 (61%)
Frame = -1 / +3
Query: 1437 GSWVY*DLLQQSLPSLVWVWWRSEIFGKIFLHQLHRVSLDIYSPLGLLYIACHLFIDREI 1258
GSW *DL QQ+L +LV + W E+ G FLH+ RVSLD LLY H+ DR+I
Sbjct: 6 GSWFC*DLFQQTLSNLVRLRWWVEVVGTFFLHKFSRVSLDFPPIDCLLYSTGHMSSDRKI 185
Query: 1257 YHSRAE*CCQPVVHVTFYLHFCYEHPRNEMEWCWN**LVEE*AVLGHWRCVLTPLCCVPG 1078
Y S + C+ VH ++H +P + M WCWN *LVEE* VLG WRC +T C P
Sbjct: 186 YCS*DKQLCKSCVHGPLHIHRSNRYP*DAMGWCWNR*LVEE*TVLGDWRCFITFFCPFPR 365
Query: 1077 TSQGHSWC*YKLHRDIKGWR**GVLRAIYIQMDYLIDTS 961
+QG SWC +KLH IK R* +L A++IQMD+ I+ S
Sbjct: 366 FAQGFSWCRHKLHCYIKSSR*WRILGALHIQMDFAINPS 482
Score = 145 bits (311), Expect = 4e-035
Identities = 68/155 (43%), Positives = 92/155 (59%)
Frame = +3 / -2
Query: 978 SPFECXXXXXXXXXSTL*CHGEACINTSYDLEKSLEHSKEV*GHTSNDPELLIPPPVINS 1157
SPFE *C+ E C++TS +LE++LE K++* TSN P+L IPPP+I S
Sbjct: 465 SPFEYVELREFSIIGCF*CNSEVCVDTS*NLEQTLEKGKKM**STSNHPKLFIPPPIIYS 286
Query: 1158 NTTPSHF*DARSKNADKK*HEPQAGNIIQLWSDKFPCQ*TDGRQCTVSQEGNRCPRIHDG 1337
NTTP H D + ++ HE + + L ++KF Q T GR TV + PRIHD
Sbjct: 285 NTTPLHLKDTGCCDGYEEGHEHKTCIVAYLRNNKFSGQKTYGR*STVDNQWEGSPRIHD* 106
Query: 1338 VDVGKSFQKFQTATIPIPKRAMIAEEDLNRPKSPP 1442
+ VGK+FQ+ Q T+ +P M AE+DLNR KSPP
Sbjct: 105 IYVGKTFQQLQPTTVAVPNWTMFAEKDLNRTKSPP 1
>gb|DQ122795.1| Medicago sativa clone M22 putative cellulose synthase catalytic
subunit mRNA, partial cds
Length = 512
Score = 129 bits (276), Expect(2) = 6e-048
Identities = 49/97 (50%), Positives = 69/97 (71%)
Frame = -2 / +2
Query: 1298 YCTLPAICLLTGKFITPELNNVASLWFMSLFICIFATSILEMRWSGVGIDDWWRNEQFWV 1119
YC LPA+ L +G+FI L+ ++ + + + + ++LE++WSG+ + DWWRNEQFW+
Sbjct: 2 YCFLPALSLFSGQFIVQSLSVTFLVFLLGITVTLCLLALLEIKWSGITLHDWWRNEQFWL 181
Query: 1118 IGGVSSHLFAVFQGLLKVIAGVDTSFTVTSKGGDDEE 1008
IGG S+H AV QGLLKVIAGVD SFT+TSK E+
Sbjct: 182 IGGTSAHPAAVLQGLLKVIAGVDISFTLTSKSATPED 292
Score = 79.0 bits (166), Expect(2) = 6e-048
Identities = 29/72 (40%), Positives = 42/72 (58%)
Frame = -2 / +2
Query: 1016 DEEFSELYTFKWXXXXXXXXXXXXLNFIGVVAGVSNAINNGYESWGPLFGKLFFAFWVIV 837
++EF++LY KW +N I + GV+ + + + W L G LFF+FWV+
Sbjct: 296 EDEFADLYLVKWSFLMVPPITIMMVNTIAIAVGVARTLYSPFPQWSRLVGGLFFSFWVLC 475
Query: 836 HLYPFLKGLVGR 801
HLYPF KGL+GR
Sbjct: 476 HLYPFAKGLLGR 511
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 36,301,116
Number of Sequences: 7669
Number of extensions: 551001
Number of successful extensions: 3187
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 47
effective length of database: 888,125
effective search space used: 1160779375
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)