TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804898.2.1
(739 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransfer... 67 9e-023
gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytran... 67 9e-023
emb|AX050436.1| Sequence 1 from Patent WO0071736 67 9e-023
gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransfer... 67 9e-023
>gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransferase (7-IOMT(9)) mRNA, complete
cds
Length = 1227
Score = 67.0 bits (140), Expect(2) = 9e-023
Identities = 23/49 (46%), Positives = 38/49 (77%)
Frame = +2 / +3
Query: 86 VAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
V E +G+ L++ GGDMF IP+ADA++LK+ILH+W + C++I+++C
Sbjct: 690 VVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKC 836
Score = 57.0 bits (118), Expect(2) = 9e-023
Identities = 20/43 (46%), Positives = 32/43 (74%)
Frame = +2 / +3
Query: 362 LSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
++ +NG ER E EW+++F+ AGF+ YKI+ G S+IE++P*
Sbjct: 954 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1082
>gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytransferase mRNA, complete cds
Length = 1231
Score = 67.0 bits (140), Expect(2) = 9e-023
Identities = 23/49 (46%), Positives = 38/49 (77%)
Frame = +2 / +1
Query: 86 VAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
V E +G+ L++ GGDMF IP+ADA++LK+ILH+W + C++I+++C
Sbjct: 691 VVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKC 837
Score = 57.0 bits (118), Expect(2) = 9e-023
Identities = 20/43 (46%), Positives = 32/43 (74%)
Frame = +2 / +1
Query: 362 LSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
++ +NG ER E EW+++F+ AGF+ YKI+ G S+IE++P*
Sbjct: 955 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1083
>emb|AX050436.1| Sequence 1 from Patent WO0071736
Length = 1231
Score = 67.0 bits (140), Expect(2) = 9e-023
Identities = 23/49 (46%), Positives = 38/49 (77%)
Frame = +2 / +1
Query: 86 VAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
V E +G+ L++ GGDMF IP+ADA++LK+ILH+W + C++I+++C
Sbjct: 691 VVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKC 837
Score = 57.0 bits (118), Expect(2) = 9e-023
Identities = 20/43 (46%), Positives = 32/43 (74%)
Frame = +2 / +1
Query: 362 LSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
++ +NG ER E EW+++F+ AGF+ YKI+ G S+IE++P*
Sbjct: 955 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1083
>gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransferase (7-IOMT(6)) mRNA, complete
cds
Length = 1284
Score = 67.0 bits (140), Expect(2) = 9e-023
Identities = 23/49 (46%), Positives = 38/49 (77%)
Frame = +2 / +3
Query: 86 VAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
V E +G+ L++ GGDMF IP+ADA++LK+ILH+W + C++I+++C
Sbjct: 696 VVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKC 842
Score = 57.0 bits (118), Expect(2) = 9e-023
Identities = 20/43 (46%), Positives = 32/43 (74%)
Frame = +2 / +3
Query: 362 LSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
++ +NG ER E EW+++F+ AGF+ YKI+ G S+IE++P*
Sbjct: 960 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1088
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,320,836
Number of Sequences: 7669
Number of extensions: 73826
Number of successful extensions: 1103
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 188073410
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)