TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1738931.2.11
(609 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514707.1|CO514707 s13dSG55B0800073_328074 Glandular tr... 207 1e-054
gb|CO513023.1|CO513023 s13dSG09G0100004_121972 Glandular tr... 204 8e-054
gb|CO514290.1|CO514290 s13dSG76G0100004_157242 Glandular tr... 172 3e-044
>gb|CO514707.1|CO514707 s13dSG55B0800073_328074 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 528
Score = 207 bits (446), Expect = 1e-054
Identities = 88/123 (71%), Positives = 96/123 (78%)
Frame = +2 / +3
Query: 71 KGGASRKEEVVTREYTINLHKRLHGCTFKKKAPNAIKEIRKFAQKAMGTTDVRIDVKLNK 250
KG A KEEVVTREYTINLHKRLHGCTFKKKAP AIKEIRKFAQ+AMGT DVR+DVKLNK
Sbjct: 102 KGAAGXKEEVVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQRAMGTNDVRVDVKLNK 281
Query: 251 HIWSSGIXXXXXXXXXXXXXXXNDEEDAKEELYSLVTVAEIPAEGLKGLGTKVVDETD*A 430
IWS GI ND+EDAKEELYSLVTV EIP E LKGLGTKV+D+ D*+
Sbjct: 282 AIWSQGIRSVPRRIRVRIARKRNDDEDAKEELYSLVTVVEIPKEELKGLGTKVIDDED*S 461
Query: 431 VAF 439
+ +
Sbjct: 462 IIY 470
Score = 108 bits (231), Expect(2) = 2e-026
Identities = 47/63 (74%), Positives = 50/63 (79%)
Frame = -1 / -3
Query: 258 QMCLLSFTSILTSVVPMAFCANFLISLMALGAFFLNVQPWRRLWRLMVYSLVTTSSFLDA 79
Q+ L S TS LTS+VPMA ANFLISL+A GAFFL V PWRRLWRLMVYS VTTSSF A
Sbjct: 289 QIALFSLTSTLTSLVPMAL*ANFLISLIAFGAFFLKVHPWRRLWRLMVYSRVTTSSFXPA 110
Query: 78 PPF 70
PF
Sbjct: 109 APF 101
Score = 24.0 bits (46), Expect(2) = 2e-026
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / -3
Query: 390 FKPSAGISATVTRE 349
F S GIS TVTRE
Sbjct: 421 FNSSLGISTTVTRE 380
>gb|CO513023.1|CO513023 s13dSG09G0100004_121972 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 530
Score = 204 bits (440), Expect = 8e-054
Identities = 87/118 (73%), Positives = 94/118 (79%)
Frame = +2 / +1
Query: 86 RKEEVVTREYTINLHKRLHGCTFKKKAPNAIKEIRKFAQKAMGTTDVRIDVKLNKHIWSS 265
RKEEVVTREYTINLHKRLHGCTFKKKAP AIKEIRKFAQKAMGT DVR+DVKLNK IWS
Sbjct: 7 RKEEVVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQKAMGTNDVRVDVKLNKAIWSQ 186
Query: 266 GIXXXXXXXXXXXXXXXNDEEDAKEELYSLVTVAEIPAEGLKGLGTKVVDETD*AVAF 439
GI ND+EDAKEELYSLVTV EIP E LKGLGTKV+D+ D*++ +
Sbjct: 187 GIRSVPRRIRVRIARKRNDDEDAKEELYSLVTVVEIPKEELKGLGTKVIDDED*SIIY 360
Score = 106 bits (227), Expect(2) = 9e-026
Identities = 46/58 (79%), Positives = 49/58 (84%)
Frame = -1 / -1
Query: 258 QMCLLSFTSILTSVVPMAFCANFLISLMALGAFFLNVQPWRRLWRLMVYSLVTTSSFL 85
Q+ L S TS LTS+VPMAF ANFLISL+A GAFFL V PWRRLWRLMVYS VTTSSFL
Sbjct: 179 QIALFSLTSTLTSLVPMAF*ANFLISLIAFGAFFLKVHPWRRLWRLMVYSRVTTSSFL 6
Score = 24.0 bits (46), Expect(2) = 9e-026
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / -1
Query: 390 FKPSAGISATVTRE 349
F S GIS TVTRE
Sbjct: 311 FNSSLGISTTVTRE 270
>gb|CO514290.1|CO514290 s13dSG76G0100004_157242 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 361
Score = 172 bits (371), Expect = 3e-044
Identities = 74/97 (76%), Positives = 76/97 (78%)
Frame = +2 / +2
Query: 71 KGGASRKEEVVTREYTINLHKRLHGCTFKKKAPNAIKEIRKFAQKAMGTTDVRIDVKLNK 250
KG A RKEEVVTREYTINLHKRLHGCTFKKKAP AIKEIRKFAQKAMGT DVR+DVKLNK
Sbjct: 71 KGAAGRKEEVVTREYTINLHKRLHGCTFKKKAPKAIKEIRKFAQKAMGTNDVRVDVKLNK 250
Query: 251 HIWSSGIXXXXXXXXXXXXXXXNDEEDAKEELYSLVT 361
IWS GI ND+EDAKEELYSLVT
Sbjct: 251 AIWSQGIRSVPRRIRVRIARKRNDDEDAKEELYSLVT 361
Score = 113 bits (242), Expect(2) = 7e-026
Identities = 49/63 (77%), Positives = 52/63 (82%)
Frame = -1 / -2
Query: 258 QMCLLSFTSILTSVVPMAFCANFLISLMALGAFFLNVQPWRRLWRLMVYSLVTTSSFLDA 79
Q+ L S TS LTS+VPMAF ANFLISL+A GAFFL V PWRRLWRLMVYS VTTSSFL A
Sbjct: 258 QIALFSLTSTLTSLVPMAF*ANFLISLIAFGAFFLKVHPWRRLWRLMVYSRVTTSSFLPA 79
Query: 78 PPF 70
PF
Sbjct: 78 APF 70
Score = 17.1 bits (31), Expect(2) = 7e-026
Identities = 5/9 (55%), Positives = 5/9 (55%)
Frame = -2 / -3
Query: 335 WHPPHRSSC 309
WH SSC
Sbjct: 335 WHLRRHSSC 309
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,757,402
Number of Sequences: 7669
Number of extensions: 59926
Number of successful extensions: 1392
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 150396379
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)