TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 1716296.2.7
         (851 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CO512850.1|CO512850  s13dSG20E0100003_121626 Glandular tr...   166   2e-042
gb|AF191305.1|AF191305  Medicago sativa glycine-rich RNA bin...   160   2e-040

>gb|CO512850.1|CO512850 s13dSG20E0100003_121626 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 398

 Score =  166 bits (358), Expect = 2e-042
 Identities = 67/83 (80%), Positives = 76/83 (91%)
 Frame = +3 / +2

                                                                       
Query: 129 ADVEYRCFVGGLAWATSNESLENAFASYGEILDSKVITDRETGRSRGFGFVTFSSENSML 308
           ADVEYRCFVGGLAWAT N++LE AF+ YGEI+DSK+I DRETGRSRGFGFVTF++E SM 
Sbjct: 83  ADVEYRCFVGGLAWATDNDALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMN 262

                                  
Query: 309 DAIENMNGKELDGRNITVNQAQS 377
           DAIE MNG++LDGRNITVNQAQS
Sbjct: 263 DAIEAMNGQDLDGRNITVNQAQS 331




 Score =  118 bits (252), Expect = 1e-027
 Identities = 56/81 (69%), Positives = 60/81 (74%)
 Frame = -2 / -3

                                                                       
Query: 370 AWLTVMLRPSSSLPFMFSIASSMEFSEEKVTKPKPRDLPVSRSVMTLESRISP*EAKAFS 191
           AWLTVMLRPS S PF+ S+AS M+FS   VT PKP D PVSRS+M LES ISP   KAFS
Sbjct: 324 AWLTVMLRPSKS*PFIASMASFMDFSLANVTNPKPLDRPVSRSLMILESTISPY*EKAFS 145

                                
Query: 190 SDSLLVAQASPPTKQRYSTSA 128
             SL VA A PPTK RYSTSA
Sbjct: 144 RASLSVAHARPPTKHRYSTSA 82



>gb|AF191305.1|AF191305 Medicago sativa glycine-rich RNA binding protein (GRP1) mRNA,
           partial cds
          Length = 318

 Score =  160 bits (344), Expect = 2e-040
 Identities = 65/83 (78%), Positives = 74/83 (89%)
 Frame = +3 / +1

                                                                       
Query: 129 ADVEYRCFVGGLAWATSNESLENAFASYGEILDSKVITDRETGRSRGFGFVTFSSENSML 308
           ADVE RCFVGGLAWAT N++LE AF+ YGEI+DSK+I DRETGRSRGFGFVTF++E SM 
Sbjct: 7   ADVEDRCFVGGLAWATDNDALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMN 186

                                  
Query: 309 DAIENMNGKELDGRNITVNQAQS 377
           D IE MNG++LDGRNITVNQAQS
Sbjct: 187 DVIEAMNGQDLDGRNITVNQAQS 255




 Score =  110 bits (235), Expect = 2e-025
 Identities = 53/81 (65%), Positives = 57/81 (70%)
 Frame = -2 / -2

                                                                       
Query: 370 AWLTVMLRPSSSLPFMFSIASSMEFSEEKVTKPKPRDLPVSRSVMTLESRISP*EAKAFS 191
           AW TVMLRPS S PF+ S+ S M+FS   VT PKP D PVSRS+M LES ISP   KAFS
Sbjct: 248 AWFTVMLRPSKS*PFIASMTSFMDFSLANVTNPKPLDRPVSRSLMILESTISPY*EKAFS 69

                                
Query: 190 SDSLLVAQASPPTKQRYSTSA 128
             SL VA A PPTK R STSA
Sbjct: 68  RASLSVAHARPPTKHRSSTSA 6



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 4,278,713
Number of Sequences: 7669
Number of extensions: 48939
Number of successful extensions: 1063
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 223279270
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)