TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131555.2.1
(1236 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular tr... 62 5e-027
emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-red... 52 3e-026
gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular tr... 61 3e-025
gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mR... 53 3e-023
>gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 602
Score = 62.0 bits (129), Expect(4) = 5e-027
Identities = 26/44 (59%), Positives = 31/44 (70%)
Frame = +2 / +1
Query: 182 KNAHLKVLEGAEERLQLLKADLMDYDSIASAVAGCEGVFHVASP 313
K HL LEGA+ERLQL KA+L++ + S V GC GVFH ASP
Sbjct: 178 KGDHLVKLEGAKERLQLFKANLLEEGAFDSVVQGCHGVFHTASP 309
Score = 45.1 bits (92), Expect(4) = 5e-027
Identities = 22/63 (34%), Positives = 28/63 (44%)
Frame = +2 / +1
Query: 413 RRVVVVSSCGAVYANPIYPKGKVFDEDCWSDEDYCRKKEDWYLVSKTLSEREALAFAAKT 592
R V+ S Y V DE ++D D C K WY+VSKTL+E A F
Sbjct: 412 RLVLTSSIAAVAYNGKPRTPDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAWKFVKVN 591
Query: 593 GLD 601
+D
Sbjct: 592 NID 600
Score = 40.9 bits (83), Expect(4) = 5e-027
Identities = 16/22 (72%), Positives = 20/22 (90%)
Frame = +2 / +1
Query: 74 VCVTGAGGFVASELVKLLLSRG 139
VCVTGA G++AS LV+LLL+RG
Sbjct: 70 VCVTGASGYIASWLVRLLLNRG 135
Score = 23.1 bits (44), Expect(4) = 5e-027
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +2 / +1
Query: 125 LLSRGQYAVRGTVRDPGASK 184
LL Y V+ TVRDP K
Sbjct: 118 LLLNRGYTVKATVRDPNDPK 177
>emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-reductase
Length = 650
Score = 52.4 bits (108), Expect(4) = 3e-026
Identities = 21/40 (52%), Positives = 29/40 (72%)
Frame = +2 / +1
Query: 536 YLVSKTLSEREALAFAAKTGLDVVTVCPSLVFGPLMQPTV 655
Y VSKTL+E+EA FA + +DV+T+ P LV GP + PT+
Sbjct: 436 YFVSKTLAEQEAWKFAKEHKMDVITIIPPLVVGPFLIPTM 555
Score = 48.3 bits (99), Expect(4) = 3e-026
Identities = 21/45 (46%), Positives = 29/45 (64%)
Frame = +2 / +1
Query: 182 KNAHLKVLEGAEERLQLLKADLMDYDSIASAVAGCEGVFHVASPV 316
K + L L GA+ +L + KADL + S A+ GC GVFHVA+P+
Sbjct: 79 KVSPLLELPGAKSKLSIWKADLGEEGSFDEAIKGCTGVFHVATPM 213
Score = 36.3 bits (73), Expect(4) = 3e-026
Identities = 13/34 (38%), Positives = 23/34 (67%)
Frame = +2 / +1
Query: 335 NPEVEVIAPAVTGTTNVLKACYEAKVRRVVVVSS 436
+PE E+I P + G +++KAC +AK R ++ +S
Sbjct: 229 DPENEMIKPTIKGVLDIMKACLKAKTVRRLIYTS 330
Score = 31.3 bits (62), Expect(4) = 3e-026
Identities = 8/15 (53%), Positives = 13/15 (86%)
Frame = +2 / +1
Query: 479 VFDEDCWSDEDYCRK 523
++DE CWSD ++CR+
Sbjct: 370 LWDESCWSDVEFCRR 414
>gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 60.6 bits (126), Expect(4) = 3e-025
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = +2 / +1
Query: 191 HLKVLEGAEERLQLLKADLMDYDSIASAVAGCEGVFHVASP 313
HL LEGA+ERLQL KA+L++ + S V GC GVFH ASP
Sbjct: 187 HLVKLEGAKERLQLFKANLLEEGAFDSVVQGCHGVFHTASP 309
Score = 42.8 bits (87), Expect(4) = 3e-025
Identities = 21/56 (37%), Positives = 26/56 (46%)
Frame = +2 / +1
Query: 413 RRVVVVSSCGAVYANPIYPKGKVFDEDCWSDEDYCRKKEDWYLVSKTLSEREALAF 580
R V+ S Y V DE ++D D C K WY+VSKTL+E A F
Sbjct: 412 RLVLTSSIAAVAYNGKPRTPDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAWKF 579
Score = 38.2 bits (77), Expect(4) = 3e-025
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +2 / +1
Query: 74 VCVTGAGGFVASELVKLLLSRG 139
VCVTGA G++AS LV LLL+RG
Sbjct: 70 VCVTGASGYIASWLV*LLLNRG 135
Score = 23.1 bits (44), Expect(4) = 3e-025
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +2 / +1
Query: 125 LLSRGQYAVRGTVRDPGASK 184
LL Y V+ TVRDP K
Sbjct: 118 LLLNRGYTVKATVRDPNDPK 177
>gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mRNA, complete cds
Length = 1237
Score = 53.3 bits (110), Expect(4) = 3e-023
Identities = 20/38 (52%), Positives = 26/38 (68%)
Frame = +2 / +2
Query: 203 LEGAEERLQLLKADLMDYDSIASAVAGCEGVFHVASPV 316
L GA E+L ADL + DS A+A+ GC G+FH ASP+
Sbjct: 206 LPGASEKLHFFNADLSNPDSFAAAIEGCVGIFHTASPI 319
Score = 41.8 bits (85), Expect(4) = 3e-023
Identities = 18/48 (37%), Positives = 24/48 (50%)
Frame = +2 / +2
Query: 722 NMVDIRDVADALLLTYEKPEASGRYICSSHAIKISDMINILKTMYPSY 865
+MV + DVA A + E GRY CS + I +M +L YP Y
Sbjct: 731 HMVHVDDVARAHIYLLENSVPGGRYNCSPFIVPIEEMSQLLSAKYPEY 874
Score = 40.0 bits (81), Expect(4) = 3e-023
Identities = 19/48 (39%), Positives = 27/48 (56%)
Frame = +2 / +2
Query: 536 YLVSKTLSEREALAFAAKTGLDVVTVCPSLVFGPLMQPTVNLSSEMIL 679
Y VSKTL+E+ L F + G+DVVT+ + G + P + S E L
Sbjct: 542 YAVSKTLAEKAVLEFGEQNGIDVVTLILPFIVGRFVCPKLPDSIEKAL 685
Score = 31.3 bits (62), Expect(4) = 3e-023
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / +2
Query: 74 VCVTGAGGFVASELVKLLLSRG 139
VCVTG GF+ S ++K LL G
Sbjct: 74 VCVTGGTGFLGSWIIKSLLENG 139
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,042,077
Number of Sequences: 7669
Number of extensions: 155840
Number of successful extensions: 1045
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 338118768
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)