TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 131537.2.8
         (708 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CO515737.1|CO515737  s13dSG77C0400034_419623 Glandular tr...   122   7e-029
gb|CO514107.1|CO514107  s13dSG75D0400030_156876 Glandular tr...   120   2e-028
gb|AJ410139.1|AJ410139  AJ410139 Medicago sativa library (Ka...   119   4e-028

>gb|CO515737.1|CO515737 s13dSG77C0400034_419623 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 541

 Score =  122 bits (260), Expect = 7e-029
 Identities = 46/75 (61%), Positives = 56/75 (74%)
 Frame = -2 / +1

                                                                       
Query: 560 GGGNGQKSKMARERNLEKNKGAKGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHAEA 381
           GG   +          ++    KGSQLE NKKAMSIQCKVCMQTF+CTT+EVKC+EHAEA
Sbjct: 88  GGNGQKAKMARERNLEKQKNAGKGSQLEKNKKAMSIQCKVCMQTFICTTSEVKCKEHAEA 267

                          
Query: 380 KHPKTDVYQCFPHLK 336
           +HPK+D++ CFPHLK
Sbjct: 268 RHPKSDLFTCFPHLK 312




 Score = 95.5 bits (202), Expect(2) = 1e-025
 Identities = 38/56 (67%), Positives = 41/56 (73%)
 Frame = +1 / -3

                                                                   
Query: 340 RWGKHWYTSVLGCLASACSRHFTSVVVHMNVCMHTLHWMLMAFLLVSSWLPLAPLF 507
           RWGK    S  GCLASACS HFTS VV +NVCMHTLHW+ +AFL  SSWLPL   F
Sbjct: 308 RWGKQVKRSDFGCLASACSLHFTSDVVQINVCMHTLHWIDIAFLFFSSWLPLPAFF 141




 Score = 35.0 bits (70), Expect(2) = 1e-025
 Identities = 14/21 (66%), Positives = 16/21 (76%)
 Frame = +1 / -3

                                
Query: 508 FSKLRSRAILDFWPLPPPMVD 570
           FS+  SRAIL F PLPPPM +
Sbjct: 137 FSRFLSRAILAF*PLPPPMAE 75



>gb|CO514107.1|CO514107 s13dSG75D0400030_156876 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 454

 Score =  120 bits (256), Expect = 2e-028
 Identities = 45/75 (60%), Positives = 56/75 (74%)
 Frame = -2 / +2

                                                                       
Query: 560 GGGNGQKSKMARERNLEKNKGAKGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHAEA 381
           GG   +          ++    KGSQLE NKKAMSIQC+VCMQTF+CTT+EVKC+EHAEA
Sbjct: 68  GGNGQKAKMARERNLEKQKNAGKGSQLEKNKKAMSIQCEVCMQTFICTTSEVKCKEHAEA 247

                          
Query: 380 KHPKTDVYQCFPHLK 336
           +HPK+D++ CFPHLK
Sbjct: 248 RHPKSDLFTCFPHLK 292




 Score = 92.7 bits (196), Expect(2) = 9e-025
 Identities = 37/56 (66%), Positives = 40/56 (71%)
 Frame = +1 / -2

                                                                   
Query: 340 RWGKHWYTSVLGCLASACSRHFTSVVVHMNVCMHTLHWMLMAFLLVSSWLPLAPLF 507
           RWGK    S  GCLASACS HFTS VV +NVCMHT HW+ +AFL  SSWLPL   F
Sbjct: 288 RWGKQVKRSDFGCLASACSLHFTSDVVQINVCMHTSHWIDIAFLFFSSWLPLPAFF 121




 Score = 35.0 bits (70), Expect(2) = 9e-025
 Identities = 14/21 (66%), Positives = 16/21 (76%)
 Frame = +1 / -2

                                
Query: 508 FSKLRSRAILDFWPLPPPMVD 570
           FS+  SRAIL F PLPPPM +
Sbjct: 117 FSRFLSRAILAF*PLPPPMAE 55



>gb|AJ410139.1|AJ410139 AJ410139 Medicago sativa library (Kalo P) Medicago sativa cDNA
           clone U594, mRNA sequence
          Length = 510

 Score =  119 bits (254), Expect = 4e-028
 Identities = 45/75 (60%), Positives = 55/75 (73%)
 Frame = -2 / +1

                                                                       
Query: 560 GGGNGQKSKMARERNLEKNKGAKGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHAEA 381
           GG   +          ++    KGSQLE N KAMSIQCKVCMQTF+CTT+EVKC+EHAEA
Sbjct: 67  GGNGQKAKMARERNLEKQKNAGKGSQLEKNXKAMSIQCKVCMQTFICTTSEVKCKEHAEA 246

                          
Query: 380 KHPKTDVYQCFPHLK 336
           +HPK+D++ CFPHLK
Sbjct: 247 RHPKSDLFTCFPHLK 291




 Score = 92.3 bits (195), Expect(2) = 1e-024
 Identities = 37/56 (66%), Positives = 40/56 (71%)
 Frame = +1 / -2

                                                                   
Query: 340 RWGKHWYTSVLGCLASACSRHFTSVVVHMNVCMHTLHWMLMAFLLVSSWLPLAPLF 507
           RWGK    S  GCLASACS HFTS VV +NVCMHTLHW+ +A L  SSWLPL   F
Sbjct: 287 RWGKQVKRSDFGCLASACSLHFTSDVVQINVCMHTLHWIDIAXLFFSSWLPLPAFF 120




 Score = 35.0 bits (70), Expect(2) = 1e-024
 Identities = 14/21 (66%), Positives = 16/21 (76%)
 Frame = +1 / -2

                                
Query: 508 FSKLRSRAILDFWPLPPPMVD 570
           FS+  SRAIL F PLPPPM +
Sbjct: 116 FSRFLSRAILAF*PLPPPMAE 54



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 6,879,097
Number of Sequences: 7669
Number of extensions: 100331
Number of successful extensions: 1487
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 178808710
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)