TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.723
(715 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512905.1|CO512905 s13dSG86B0800061_121736 Glandular tr... 26 0.25
gb|AW698339.1|AW698339 g122 glandular-haired subtracted cDN... 30 0.26
gb|CO512442.1|CO512442 s13dSG80H1200096_114398 Glandular tr... 30 0.35
gb|CO516689.1|CO516689 s13dSG74B1000079_446706 Glandular tr... 29 0.48
gb|CO512621.1|CO512621 s13dSG13D1000078_121168 Glandular tr... 28 1.7
emb|AJ248327.1|MSA248327 Medicago sativa subsp. X varia mRN... 28 1.7
gb|CO516559.1|CO516559 s13dSG57E0700053_446446 Glandular tr... 27 2.4
gb|CO512318.1|CO512318 s13dSG68E1100083_114150 Glandular tr... 27 3.3
emb|CS137804.1| Sequence 775 from Patent WO2005047516 27 3.3
gb|CO513646.1|CO513646 s13dSG17B0700057_140074 Glandular tr... 23 3.3
gb|CO514137.1|CO514137 s13dSG75F1100091_156936 Glandular tr... 26 4.5
gb|CO514505.1|CO514505 s13dSG43E0600049_327670 Glandular tr... 26 4.5
gb|CO516214.1|CO516214 s13dSG67G0900072_445756 Glandular tr... 26 4.5
gb|CO516274.1|CO516274 s13dSG69E0100003_445876 Glandular tr... 26 4.5
gb|CO517279.1|CO517279 s13dSG45A0500037_486154 Glandular tr... 26 4.5
gb|AY681973.1| Medicago sativa putative defensin 2.1 precur... 26 4.5
gb|CO512209.1|CO512209 s13dSG03C0900066_113932 Glandular tr... 26 6.1
gb|CO514853.1|CO514853 s13dSG56B0400041_328366 Glandular tr... 26 6.1
gb|CO515504.1|CO515504 s13dSG52D0900078_418067 Glandular tr... 26 6.1
gb|CO515756.1|CO515756 s13dSG77D1200102_419661 Glandular tr... 26 6.1
gb|CB858158.1|CB858158 RX99 Medicago sativa cDNA-AFLP Medic... 25 8.4
emb|AJ306690.1|MSA306690 Medicago sativa prx2 gene for pero... 25 8.4
>gb|CO512905.1|CO512905 s13dSG86B0800061_121736 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 590
Score = 26.3 bits (51), Expect(2) = 0.25
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2 / +3
Query: 8 LNLLMKINAKLGGLNSLLQVEASSSIPH 91
LN+ + KL LNSL + SS+PH
Sbjct: 213 LNVTTATSEKLTALNSLTLLPNFSSVPH 296
Score = 23.1 bits (44), Expect(2) = 0.25
Identities = 11/28 (39%), Positives = 12/28 (42%)
Frame = +3 / +1
Query: 369 MESVKVSLPRSSTLSWIRSSRHVSFWMR 452
M SL S + W R S H S W R
Sbjct: 421 MGKTSPSLRHSPSS*WFRRSCHWSSWFR 504
>gb|AW698339.1|AW698339 g122 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 390
Score = 30.4 bits (60), Expect = 0.26
Identities = 14/36 (38%), Positives = 18/36 (50%)
Frame = +1 / +1
Query: 586 LLHVCTCWDDWNNKAHPLSCSARRDRFLCRRDAGVC 693
LLH + W +K H LS +A + FLC G C
Sbjct: 277 LLHFLSNWTSQYHKPH*LSETAPHNLFLCTHLDGTC 384
>gb|CO512442.1|CO512442 s13dSG80H1200096_114398 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 438
Score = 29.9 bits (59), Expect = 0.35
Identities = 9/30 (30%), Positives = 17/30 (56%)
Frame = +2 / +2
Query: 425 IEACKFLDEKWSPKFTVIVAQKNHHTKFFQ 514
++ CKF + WS + ++ +N H K F+
Sbjct: 158 VKMCKFSGQSWSSRVPPLLCHQNIHVKGFE 247
>gb|CO516689.1|CO516689 s13dSG74B1000079_446706 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 29.5 bits (58), Expect = 0.48
Identities = 11/33 (33%), Positives = 16/33 (48%)
Frame = +2 / -3
Query: 143 PGQDRPSVAAVVSSRQWPLISRYRASVHTQSAR 241
PGQ RPS+ S+ P YR+ + + R
Sbjct: 450 PGQSRPSIKCCASATSRPTFPLYRSGIRALNPR 352
>gb|CO512621.1|CO512621 s13dSG13D1000078_121168 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 599
Score = 27.6 bits (54), Expect = 1.7
Identities = 17/53 (32%), Positives = 24/53 (45%)
Frame = -1 / +1
Query: 685 LHLVCRETYLVVQNMIVGGPCCSNHPSMCTHVEVEVLRMANFTIHHSSRKNIV 527
LHL ++ +L V N++ S CTH E T+ SS KN+V
Sbjct: 31 LHLTKQQQWLFVTNLLPSTALSSIWMIPCTHPTPESELPLRKTLISSSWKNVV 189
>emb|AJ248327.1|MSA248327 Medicago sativa subsp. X varia mRNA for L3 Ribosomal protein
Length = 1201
Score = 27.6 bits (54), Expect = 1.7
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +3 / -2
Query: 372 ESVKVSLPRSSTLSWIRSSRHVSF 443
ES+ S SSTLSW +S R VS+
Sbjct: 117 ESLHFSSVTSSTLSWEKSQRTVSW 46
>gb|CO516559.1|CO516559 s13dSG57E0700053_446446 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 496
Score = 27.2 bits (53), Expect = 2.4
Identities = 8/37 (21%), Positives = 18/37 (48%)
Frame = -1 / +2
Query: 637 VGGPCCSNHPSMCTHVEVEVLRMANFTIHHSSRKNIV 527
+G P C+ HP+ C + + + + + SR ++
Sbjct: 200 IGVPMCTMHPNFCIVMYISYQKQVIYGSYQESRAGLI 310
>gb|CO512318.1|CO512318 s13dSG68E1100083_114150 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 437
Score = 26.7 bits (52), Expect = 3.3
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -1 / -2
Query: 217 CSISRDKRPLTRTNH 173
CS++ D PLT TNH
Sbjct: 256 CSLASDAHPLTFTNH 212
>emb|CS137804.1| Sequence 775 from Patent WO2005047516
Length = 859
Score = 26.7 bits (52), Expect = 3.3
Identities = 6/14 (42%), Positives = 11/14 (78%)
Frame = +1 / -2
Query: 577 ELRLLHVCTCWDDW 618
EL ++++C CW +W
Sbjct: 153 ELLVVNICMCWREW 112
>gb|CO513646.1|CO513646 s13dSG17B0700057_140074 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 332
Score = 23.1 bits (44), Expect(2) = 3.3
Identities = 8/22 (36%), Positives = 12/22 (54%)
Frame = +1 / +1
Query: 73 IFVNTTCVASTHHHLRYGCFTW 138
+FVN+ C H+ L+ TW
Sbjct: 154 LFVNSKCKRYFHNKLKPSKLTW 219
Score = 20.3 bits (38), Expect(2) = 3.3
Identities = 6/10 (60%), Positives = 7/10 (70%)
Frame = +1 / +3
Query: 451 EVVTQVHCDC 480
E + QVHC C
Sbjct: 294 EAILQVHCWC 323
>gb|CO514137.1|CO514137 s13dSG75F1100091_156936 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 540
Score = 26.3 bits (51), Expect = 4.5
Identities = 9/20 (45%), Positives = 10/20 (50%)
Frame = -2 / +1
Query: 93 TCGIDEDASTCSNEFRPPSF 34
TC D +TC N F SF
Sbjct: 184 TCSYSFDTTTCGNSFNTTSF 243
>gb|CO514505.1|CO514505 s13dSG43E0600049_327670 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 616
Score = 26.3 bits (51), Expect = 4.5
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -1 / -2
Query: 700 RMNKLLHLVCRETYLV 653
++ KLLH+V R TYLV
Sbjct: 462 KIYKLLHMVTRTTYLV 415
>gb|CO516214.1|CO516214 s13dSG67G0900072_445756 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 502
Score = 26.3 bits (51), Expect = 4.5
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -1 / -1
Query: 700 RMNKLLHLVCRETYLV 653
++ KLLH+V R TYLV
Sbjct: 316 KIYKLLHMVTRTTYLV 269
>gb|CO516274.1|CO516274 s13dSG69E0100003_445876 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 312
Score = 26.3 bits (51), Expect = 4.5
Identities = 9/20 (45%), Positives = 10/20 (50%)
Frame = -2 / +3
Query: 93 TCGIDEDASTCSNEFRPPSF 34
TC D +TC N F SF
Sbjct: 183 TCSYSFDTTTCGNSFNTTSF 242
>gb|CO517279.1|CO517279 s13dSG45A0500037_486154 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 583
Score = 26.3 bits (51), Expect = 4.5
Identities = 7/18 (38%), Positives = 12/18 (66%)
Frame = +1 / +1
Query: 586 LLHVCTCWDDWNNKAHPL 639
L+H +CW W++ HP+
Sbjct: 235 LVHNLSCW*SWHSIFHPM 288
>gb|AY681973.1| Medicago sativa putative defensin 2.1 precursor (Def2.1) gene,
complete cds
Length = 564
Score = 26.3 bits (51), Expect = 4.5
Identities = 10/39 (25%), Positives = 18/39 (46%)
Frame = -1 / -1
Query: 676 VCRETYLVVQNMIVGGPCCSNHPSMCTHVEVEVLRMANF 560
+C + + ++ PC S H C HV + +R+ F
Sbjct: 474 ICEAWSSICISQMLTSPCFSYHNFFCIHVPLNNIRIYFF 358
>gb|CO512209.1|CO512209 s13dSG03C0900066_113932 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 597
Score = 25.8 bits (50), Expect = 6.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3 / -2
Query: 336 SENQNT*LFSGMESVKVSLPRSSTLSWIRSSR 431
+ENQ + G+ + S PR +W+RSS+
Sbjct: 386 AENQLYPILVGLSPLATSHPRILPHTWVRSSK 291
>gb|CO514853.1|CO514853 s13dSG56B0400041_328366 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 583
Score = 25.8 bits (50), Expect = 6.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3 / -1
Query: 336 SENQNT*LFSGMESVKVSLPRSSTLSWIRSSR 431
+ENQ + G+ + S PR +W+RSS+
Sbjct: 346 AENQLYPILVGLSPLATSHPRILPHTWVRSSK 251
>gb|CO515504.1|CO515504 s13dSG52D0900078_418067 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 491
Score = 25.8 bits (50), Expect = 6.1
Identities = 8/19 (42%), Positives = 11/19 (57%)
Frame = +1 / +2
Query: 631 HPLSCSARRDRFLCRRDAG 687
HP +C RR RF+ + G
Sbjct: 59 HPTNCHCRRQRFIISHNRG 115
>gb|CO515756.1|CO515756 s13dSG77D1200102_419661 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 556
Score = 25.8 bits (50), Expect = 6.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3 / -2
Query: 336 SENQNT*LFSGMESVKVSLPRSSTLSWIRSSR 431
+ENQ + G+ + S PR +W+RSS+
Sbjct: 369 AENQLYPILVGLSPLATSHPRILPHTWVRSSK 274
>gb|CB858158.1|CB858158 RX99 Medicago sativa cDNA-AFLP Medicago sativa cDNA, mRNA sequence
Length = 583
Score = 25.4 bits (49), Expect = 8.4
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -3 / -3
Query: 614 SSQHVHTCRSRSS*DG 567
SS HVH SRSS* G
Sbjct: 425 SSSHVHDISSRSS*SG 378
>emb|AJ306690.1|MSA306690 Medicago sativa prx2 gene for peroxidase2, exons 1-4
Length = 5090
Score = 25.4 bits (49), Expect = 8.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -2 / -3
Query: 510 KNLVWWFF*ATITVNLGDHFSSRNL 436
+N++ W +NL DH S RNL
Sbjct: 3483 QNILKWSLCFLFYLNLTDHLSKRNL 3409
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,344,728
Number of Sequences: 7669
Number of extensions: 109805
Number of successful extensions: 1129
Number of sequences better than 10.0: 24
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 180661650
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)