TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.65
(673 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular tr... 198 6e-052
gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular tr... 195 4e-051
gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular tr... 160 2e-040
gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular tr... 137 2e-033
gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular tr... 124 1e-029
>gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 521
Score = 198 bits (427), Expect = 6e-052
Identities = 86/93 (92%), Positives = 91/93 (97%)
Frame = +3 / +2
Query: 261 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR 440
SVETYKIYIFKVLKQVHPDIG+SSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSR
Sbjct: 206 SVETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSR 385
Query: 441 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 539
EIQT+VRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 386 EIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS* 484
Score = 120 bits (256), Expect = 2e-028
Identities = 59/95 (62%), Positives = 66/95 (69%)
Frame = -1 / -3
Query: 529 VNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLMKEF 350
VNLVTALVPSETACLA+SPGRT T V IS EV+VGF SK+SLMK F
Sbjct: 474 VNLVTALVPSETACLANSPGRTNLTAV*ISLEVIVGFLLYLAKRDDS*ASFSKISLMKLF 295
Query: 349 MMDMALEEMPMSGCTCFSTLKM*ILYVSTLFLAFF 245
++ +AL E P+SG TCF TLKM ILYVSTLF F
Sbjct: 294 IIPIALLETPISG*TCFRTLKMYILYVSTLFFVLF 190
>gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 566
Score = 195 bits (421), Expect = 4e-051
Identities = 86/93 (92%), Positives = 90/93 (96%)
Frame = +3 / +1
Query: 261 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR 440
SVETYKIYIFK LKQVHPDIGISSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSR
Sbjct: 244 SVETYKIYIFKGLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSR 423
Query: 441 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 539
EIQT+VRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 424 EIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS* 522
Score = 123 bits (263), Expect = 2e-029
Identities = 62/90 (68%), Positives = 66/90 (73%)
Frame = -1 / -1
Query: 529 VNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLMKEF 350
VNLVTALVPSETACLASSPGRTRRT V ISREVMVGF SK+SLMK F
Sbjct: 512 VNLVTALVPSETACLASSPGRTRRTAV*ISREVMVGFLLYLASLEDS*ASFSKISLMKLF 333
Query: 349 MMDMALEEMPMSGCTCFSTLKM*ILYVSTL 260
M+ +AL E+PMSG TC LKM*IL VSTL
Sbjct: 332 MIPIALLEIPMSG*TCLRPLKM*IL*VSTL 243
>gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 500
Score = 160 bits (344), Expect = 2e-040
Identities = 69/75 (92%), Positives = 73/75 (97%)
Frame = +3 / +2
Query: 261 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR 440
SVETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSR
Sbjct: 260 SVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSR 439
Query: 441 EIQTSVRLVLPGELA 485
EIQT+VRLVLPGELA
Sbjct: 440 EIQTAVRLVLPGELA 484
Score = 99.1 bits (210), Expect(2) = 1e-023
Identities = 51/84 (60%), Positives = 57/84 (67%)
Frame = -1 / -3
Query: 496 TACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLMKEFMMDMALEEMPM 317
++ LA+SPGRT T V IS EV+VGF SKMSLMK FM+ MAL E+PM
Sbjct: 495 SSMLANSPGRTSLTAV*ISLEVIVGFLL*RASLDDS*ASFSKMSLMKLFMIPMALLEIPM 316
Query: 316 SGCTCFSTLKM*ILYVSTLFLAFF 245
SG TCF TLKM*ILYVSTLFL F
Sbjct: 315 SG*TCFRTLKM*ILYVSTLFLFLF 244
Score = 24.4 bits (47), Expect(2) = 1e-023
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -1 / -3
Query: 262 LFLAFFRPFLSTPPSLPADFP 200
LFL FF + PPSL + FP
Sbjct: 255 LFLFFFLSPAAEPPSLGSFFP 193
>gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 363
Score = 137 bits (293), Expect = 2e-033
Identities = 60/65 (92%), Positives = 64/65 (98%)
Frame = +3 / +2
Query: 345 IMNSFINDIFEKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTK 524
IMNSFINDIFEKLA E+++LARYNKKPTITSREIQT+VRLVLPGELAKHAVSEGTKAVTK
Sbjct: 8 IMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTK 187
Query: 525 FTSS* 539
FTSS*
Sbjct: 188 FTSS* 202
>gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 403
Score = 124 bits (265), Expect = 1e-029
Identities = 56/78 (71%), Positives = 59/78 (75%)
Frame = +3 / +2
Query: 198 AGKSAGKEGGVDXXXXXXXXXSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFE 377
A K KE SVETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIFE
Sbjct: 170 AEKKIPKEASSTDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFE 349
Query: 378 KLAGEAAKLARYNKKPTI 431
KLA E+++LARYNKKPTI
Sbjct: 350 KLAQESSRLARYNKKPTI 403
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,244,002
Number of Sequences: 7669
Number of extensions: 37643
Number of successful extensions: 4578
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 167691070
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)