TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.462
(823 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular tr... 197 2e-051
gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular tr... 194 1e-050
gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular tr... 158 7e-040
gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular tr... 135 6e-033
gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular tr... 121 1e-028
>gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 521
Score = 197 bits (424), Expect = 2e-051
Identities = 85/95 (89%), Positives = 91/95 (95%)
Frame = +2 / +2
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 442
VETYKIYIFKVLKQVHPDIG+SSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSRE
Sbjct: 209 VETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 388
Query: 443 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSN*ARC 547
IQT+VRLVLPGELAKHAVSEGTKAVTKFTS+* C
Sbjct: 389 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*FCC 493
Score = 124 bits (266), Expect = 1e-029
Identities = 61/91 (67%), Positives = 71/91 (78%)
Frame = -2 / -3
Query: 546 QRAQLLVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKM 367
Q+ Q LVNLVTALVPSETACLA+SPGRT T V IS +V+VGFLLY A S ASFSK+
Sbjct: 492 QQNQELVNLVTALVPSETACLANSPGRTNLTAV*ISLEVIVGFLLYLAKRDDS*ASFSKI 313
Query: 366 SLMKEFMMDMALEDMPMSGCTCFSTLKM*IL 274
SLMK F++ +AL + P+SG TCF TLKM IL
Sbjct: 312 SLMKLFIIPIALLETPISG*TCFRTLKMYIL 220
>gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 566
Score = 194 bits (418), Expect = 1e-050
Identities = 85/94 (90%), Positives = 90/94 (95%)
Frame = +2 / +1
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 442
VETYKIYIFK LKQVHPDIGISSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSRE
Sbjct: 247 VETYKIYIFKGLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 426
Query: 443 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSN*AR 544
IQT+VRLVLPGELAKHAVSEGTKAVTKFTS+* R
Sbjct: 427 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*MR 528
Score = 147 bits (315), Expect = 2e-036
Identities = 74/102 (72%), Positives = 81/102 (79%)
Frame = -2 / -1
Query: 573 TLLTDPRIPQRAQLLVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLA 394
TL+ R R Q LVNLVTALVPSETACLASSPGRTRRT V ISR+VMVGFLLY ASL
Sbjct: 557 TLII*TREMYRIQELVNLVTALVPSETACLASSPGRTRRTAV*ISREVMVGFLLYLASLE 378
Query: 393 ASPASFSKMSLMKEFMMDMALEDMPMSGCTCFSTLKM*IL*V 268
S ASFSK+SLMK FM+ +AL ++PMSG TC LKM*IL*V
Sbjct: 377 DS*ASFSKISLMKLFMIPIALLEIPMSG*TCLRPLKM*IL*V 252
>gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 500
Score = 158 bits (340), Expect = 7e-040
Identities = 68/74 (91%), Positives = 72/74 (97%)
Frame = +2 / +2
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 442
VETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSRE
Sbjct: 263 VETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 442
Query: 443 IQTSVRLVLPGELA 484
IQT+VRLVLPGELA
Sbjct: 443 IQTAVRLVLPGELA 484
Score = 101 bits (216), Expect = 9e-023
Identities = 51/74 (68%), Positives = 59/74 (79%)
Frame = -2 / -3
Query: 495 TACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKEFMMDMALEDMPM 316
++ LA+SPGRT T V IS +V+VGFLL RASL S ASFSKMSLMK FM+ MAL ++PM
Sbjct: 495 SSMLANSPGRTSLTAV*ISLEVIVGFLL*RASLDDS*ASFSKMSLMKLFMIPMALLEIPM 316
Query: 315 SGCTCFSTLKM*IL 274
SG TCF TLKM*IL
Sbjct: 315 SG*TCFRTLKM*IL 274
>gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 363
Score = 135 bits (290), Expect = 6e-033
Identities = 59/65 (90%), Positives = 64/65 (98%)
Frame = +2 / +2
Query: 344 IMNSFINDIFEKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTK 523
IMNSFINDIFEKLA E+++LARYNKKPTITSREIQT+VRLVLPGELAKHAVSEGTKAVTK
Sbjct: 8 IMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTK 187
Query: 524 FTSN* 538
FTS+*
Sbjct: 188 FTSS* 202
Score = 99.6 bits (211), Expect = 5e-022
Identities = 49/63 (77%), Positives = 53/63 (84%)
Frame = -2 / -1
Query: 531 LVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKE 352
LVNLVTALVPSETACLA+SPGRT T V IS +V+VGFLL RASL S ASFSKMSLMK
Sbjct: 195 LVNLVTALVPSETACLANSPGRTSLTAV*ISLEVIVGFLL*RASLDDS*ASFSKMSLMKL 16
Query: 351 FMM 343
FM+
Sbjct: 15 FMI 7
>gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 403
Score = 121 bits (259), Expect = 1e-028
Identities = 51/56 (91%), Positives = 54/56 (96%)
Frame = +2 / +2
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTI 430
VETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIFEKLA E+++LARYNKKPTI
Sbjct: 236 VETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTI 403
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,531,731
Number of Sequences: 7669
Number of extensions: 32619
Number of successful extensions: 362
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 214014570
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)