TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.387
(908 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA... 182 8e-085
gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA... 182 8e-085
gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, comple... 182 3e-070
gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular tr... 69 5e-022
>gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA, complete cds
Length = 1267
Score = 182 bits (392), Expect(3) = 8e-085
Identities = 67/95 (70%), Positives = 80/95 (84%)
Frame = -3 / +2
Query: 738 YYGRGPIQLTHKYNYRLAGQALNLNLVGDPDLVARDPVVAFKTAIWFWMTPQSPKPSCHA 559
YYGRGPIQ++ YNY G+A+ ++L+ +PDLVA DPV++FKTA+WFWMTPQSPKPSCH
Sbjct: 572 YYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHD 751
Query: 558 VMTGDWRPSATDRAAGRLPGYGLTTNIINGGLECG 454
V+TG W PS+ DRAAGRL GYG TNIINGGLECG
Sbjct: 752 VITGRWSPSSADRAAGRLSGYGTVTNIINGGLECG 856
Score = 110 bits (235), Expect(3) = 2e-049
Identities = 46/66 (69%), Positives = 50/66 (75%)
Frame = +2 / -2
Query: 455 PHSRPPLMMFVVRPYPGSLPAARSVAEGLQSPVITAWHEGLGDCGVIQNQMAVLKATTGS 634
PHS PP +M V PYP SLPAARS EGL PVIT+W +GLGD GVIQN AVLK TGS
Sbjct: 855 PHSSPPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGS 676
Query: 635 LATRSG 652
+ATRSG
Sbjct: 675 VATRSG 658
Score = 104 bits (222), Expect(3) = 8e-085
Identities = 37/50 (74%), Positives = 42/50 (84%)
Frame = -3 / +2
Query: 906 FPGFGTTGDEQTRRRELAAFFGQTSHETTGGWATAPDGPFAWGYCRVKEQ 757
FP F GD T++RE+AAF GQTSHETTGGWATAPDGP+AWGYC V+EQ
Sbjct: 365 FPNFANNGDTATKKREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVREQ 514
Score = 83.5 bits (176), Expect(3) = 2e-049
Identities = 36/51 (70%), Positives = 39/51 (76%)
Frame = +2 / -2
Query: 755 FCSFTRQYPQANGPSGAVAHPPVVSWDVWPKKAASSRLRVCSSPVVPKPGN 907
FCS T+QYPQA GPSGAVAHPPVVS DV PKKAA S V SP++ K GN
Sbjct: 516 FCSLTKQYPQAYGPSGAVAHPPVVSCDV*PKKAAISLFLVAVSPLLAKLGN 364
Score = 61.1 bits (127), Expect(3) = 8e-085
Identities = 20/31 (64%), Positives = 27/31 (87%)
Frame = -3 / +2
Query: 420 KDRVGYYKRYCGMLGVGYGDNMACGSQKPYG 328
+DR+G+YKRYC +LGVGYG N+ C SQ+P+G
Sbjct: 878 QDRIGFYKRYCDILGVGYGANLDCFSQRPFG 970
Score = 40.5 bits (82), Expect(3) = 2e-049
Identities = 20/43 (46%), Positives = 25/43 (58%)
Frame = +2 / -2
Query: 329 P*GFWLPHAMLSPYPTPSIPQYLL**PTRSLAALAPSVERSSP 457
P G + L+PYPTPS+ QYLL* P RS + + SSP
Sbjct: 969 PKGL*EKQSRLAPYPTPSMSQYLL*NPIRSCTLPSCPLPHSSP 841
>gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA, complete cds
Length = 1259
Score = 182 bits (392), Expect(3) = 8e-085
Identities = 67/95 (70%), Positives = 80/95 (84%)
Frame = -3 / +1
Query: 738 YYGRGPIQLTHKYNYRLAGQALNLNLVGDPDLVARDPVVAFKTAIWFWMTPQSPKPSCHA 559
YYGRGPIQ++ YNY G+A+ ++L+ +PDLVA DPV++FKTA+WFWMTPQSPKPSCH
Sbjct: 571 YYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHD 750
Query: 558 VMTGDWRPSATDRAAGRLPGYGLTTNIINGGLECG 454
V+TG W PS+ DRAAGRL GYG TNIINGGLECG
Sbjct: 751 VITGRWSPSSADRAAGRLSGYGTVTNIINGGLECG 855
Score = 110 bits (235), Expect(3) = 2e-049
Identities = 46/66 (69%), Positives = 50/66 (75%)
Frame = +2 / -1
Query: 455 PHSRPPLMMFVVRPYPGSLPAARSVAEGLQSPVITAWHEGLGDCGVIQNQMAVLKATTGS 634
PHS PP +M V PYP SLPAARS EGL PVIT+W +GLGD GVIQN AVLK TGS
Sbjct: 854 PHSSPPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGS 675
Query: 635 LATRSG 652
+ATRSG
Sbjct: 674 VATRSG 657
Score = 104 bits (222), Expect(3) = 8e-085
Identities = 37/50 (74%), Positives = 42/50 (84%)
Frame = -3 / +1
Query: 906 FPGFGTTGDEQTRRRELAAFFGQTSHETTGGWATAPDGPFAWGYCRVKEQ 757
FP F GD T++RE+AAF GQTSHETTGGWATAPDGP+AWGYC V+EQ
Sbjct: 364 FPNFANNGDTATKKREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVREQ 513
Score = 83.5 bits (176), Expect(3) = 2e-049
Identities = 36/51 (70%), Positives = 39/51 (76%)
Frame = +2 / -1
Query: 755 FCSFTRQYPQANGPSGAVAHPPVVSWDVWPKKAASSRLRVCSSPVVPKPGN 907
FCS T+QYPQA GPSGAVAHPPVVS DV PKKAA S V SP++ K GN
Sbjct: 515 FCSLTKQYPQAYGPSGAVAHPPVVSCDV*PKKAAISLFLVAVSPLLAKLGN 363
Score = 61.1 bits (127), Expect(3) = 8e-085
Identities = 20/31 (64%), Positives = 27/31 (87%)
Frame = -3 / +1
Query: 420 KDRVGYYKRYCGMLGVGYGDNMACGSQKPYG 328
+DR+G+YKRYC +LGVGYG N+ C SQ+P+G
Sbjct: 877 QDRIGFYKRYCDILGVGYGANLDCFSQRPFG 969
Score = 40.5 bits (82), Expect(3) = 2e-049
Identities = 20/43 (46%), Positives = 25/43 (58%)
Frame = +2 / -1
Query: 329 P*GFWLPHAMLSPYPTPSIPQYLL**PTRSLAALAPSVERSSP 457
P G + L+PYPTPS+ QYLL* P RS + + SSP
Sbjct: 968 PKGL*EKQSRLAPYPTPSMSQYLL*NPIRSCTLPSCPLPHSSP 840
>gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, complete cds
Length = 987
Score = 182 bits (392), Expect(4) = 3e-070
Identities = 67/95 (70%), Positives = 80/95 (84%)
Frame = -3 / +1
Query: 738 YYGRGPIQLTHKYNYRLAGQALNLNLVGDPDLVARDPVVAFKTAIWFWMTPQSPKPSCHA 559
YYGRGPIQ++ YNY G+A+ ++L+ +PDLVA DPV++FKTA+WFWMTPQSPKPSCH
Sbjct: 541 YYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHD 720
Query: 558 VMTGDWRPSATDRAAGRLPGYGLTTNIINGGLECG 454
V+TG W PS+ DRAAGRL GYG TNIINGGLECG
Sbjct: 721 VITGRWSPSSADRAAGRLSGYGTVTNIINGGLECG 825
Score = 110 bits (235), Expect(3) = 3e-038
Identities = 46/66 (69%), Positives = 50/66 (75%)
Frame = +2 / -2
Query: 455 PHSRPPLMMFVVRPYPGSLPAARSVAEGLQSPVITAWHEGLGDCGVIQNQMAVLKATTGS 634
PHS PP +M V PYP SLPAARS EGL PVIT+W +GLGD GVIQN AVLK TGS
Sbjct: 824 PHSSPPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGS 645
Query: 635 LATRSG 652
+ATRSG
Sbjct: 644 VATRSG 627
Score = 61.1 bits (127), Expect(4) = 3e-070
Identities = 20/31 (64%), Positives = 27/31 (87%)
Frame = -3 / +1
Query: 420 KDRVGYYKRYCGMLGVGYGDNMACGSQKPYG 328
+DR+G+YKRYC +LGVGYG N+ C SQ+P+G
Sbjct: 847 QDRIGFYKRYCDILGVGYGANLDCFSQRPFG 939
Score = 46.9 bits (96), Expect(4) = 3e-070
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = -3 / +1
Query: 816 GWATAPDGPFAWGYCRVKEQ 757
G ATAPDGP+AWGYC V+EQ
Sbjct: 424 GGATAPDGPYAWGYCFVREQ 483
Score = 45.1 bits (92), Expect(3) = 3e-038
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +2 / -2
Query: 755 FCSFTRQYPQANGPSGAVAHP 817
FCS T+QYPQA GPSGAVA P
Sbjct: 485 FCSLTKQYPQAYGPSGAVAPP 423
Score = 40.5 bits (82), Expect(3) = 3e-038
Identities = 20/43 (46%), Positives = 25/43 (58%)
Frame = +2 / -2
Query: 329 P*GFWLPHAMLSPYPTPSIPQYLL**PTRSLAALAPSVERSSP 457
P G + L+PYPTPS+ QYLL* P RS + + SSP
Sbjct: 938 PKGL*EKQSRLAPYPTPSMSQYLL*NPIRSCTLPSCPLPHSSP 810
Score = 27.2 bits (53), Expect(4) = 3e-070
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -3 / +2
Query: 870 RRRELAAFFGQTSHE 826
++ +AAF GQTSHE
Sbjct: 368 KKERIAAFLGQTSHE 412
>gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 510
Score = 69.3 bits (145), Expect(2) = 5e-022
Identities = 25/51 (49%), Positives = 35/51 (68%)
Frame = -3 / +1
Query: 738 YYGRGPIQLTHKYNYRLAGQALNLNLVGDPDLVARDPVVAFKTAIWFWMTP 586
YYGRG I + YNY AG+AL +NL+ P+ + ++ +AF+ AIW WMTP
Sbjct: 70 YYGRGAIPIYWNYNYGAAGEALKVNLLDHPEYIEQNATLAFQAAIWKWMTP 222
Score = 49.6 bits (102), Expect(2) = 5e-022
Identities = 17/42 (40%), Positives = 25/42 (59%)
Frame = -3 / +1
Query: 576 KPSCHAVMTGDWRPSATDRAAGRLPGYGLTTNIINGGLECGE 451
+PS H G+W+P+ D R+PG+G T NI+ G CG+
Sbjct: 235 QPSAHDAFVGNWKPTKNDTMEKRVPGFGATMNILYGEGVCGQ 360
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,100,988
Number of Sequences: 7669
Number of extensions: 94934
Number of successful extensions: 1140
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 237969459
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)