TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.355
(842 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular tr... 180 2e-046
gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular tr... 177 1e-045
gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular tr... 142 7e-035
gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular tr... 120 2e-028
gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular tr... 105 7e-024
>gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 521
Score = 180 bits (387), Expect = 2e-046
Identities = 80/93 (86%), Positives = 82/93 (88%)
Frame = -2 / +2
Query: 595 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSR 416
SVETYKIYIFKVLKQVHPDIG+SSKAM IMNSFINDIF ARYNKKPTITSR
Sbjct: 206 SVETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSR 385
Query: 415 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 317
EIQT+VRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 386 EIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS* 484
>gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 566
Score = 177 bits (382), Expect = 1e-045
Identities = 80/94 (85%), Positives = 82/94 (87%)
Frame = -2 / +1
Query: 595 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSR 416
SVETYKIYIFK LKQVHPDIGISSKAM IMNSFINDIF ARYNKKPTITSR
Sbjct: 244 SVETYKIYIFKGLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSR 423
Query: 415 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*I 314
EIQT+VRLVLPGELAKHAVSEGTKAVTKFTSS*+
Sbjct: 424 EIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*M 525
Score = 76.7 bits (161), Expect(2) = 2e-023
Identities = 35/38 (92%), Positives = 35/38 (92%)
Frame = +3 / -1
Query: 321 EEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVG 434
E VNLVTALVPSETACLASSPGRTRRT V ISREVMVG
Sbjct: 518 ELVNLVTALVPSETACLASSPGRTRRTAV*ISREVMVG 405
Score = 46.9 bits (96), Expect(2) = 2e-023
Identities = 18/29 (62%), Positives = 23/29 (79%)
Frame = +2 / -3
Query: 485 DVVDEGVHDGHGLGRDTNVRVHLLEHLED 571
D++DE VHD H L DT+VR++LLE LED
Sbjct: 354 DIIDEAVHDPHSLA*DTDVRMNLLETLED 268
>gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 500
Score = 142 bits (304), Expect = 7e-035
Identities = 63/75 (84%), Positives = 64/75 (85%)
Frame = -2 / +2
Query: 595 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSR 416
SVETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIF ARYNKKPTITSR
Sbjct: 260 SVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSR 439
Query: 415 EIQTSVRLVLPGELA 371
EIQT+VRLVLPGELA
Sbjct: 440 EIQTAVRLVLPGELA 484
>gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 363
Score = 120 bits (257), Expect = 2e-028
Identities = 55/66 (83%), Positives = 56/66 (84%)
Frame = -2 / +2
Query: 511 IMNSFINDIFXXXXXXXXXXARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTK 332
IMNSFINDIF ARYNKKPTITSREIQT+VRLVLPGELAKHAVSEGTKAVTK
Sbjct: 8 IMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTK 187
Query: 331 FTSS*I 314
FTSS*I
Sbjct: 188 FTSS*I 205
>gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 403
Score = 105 bits (224), Expect = 7e-024
Identities = 48/75 (64%), Positives = 50/75 (66%)
Frame = -2 / +2
Query: 649 RVPXXXXXXXXXXXXXXKSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXX 470
++P KSVETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIF
Sbjct: 179 KIPKEASSTDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLA 358
Query: 469 XXXXXXARYNKKPTI 425
ARYNKKPTI
Sbjct: 359 QESSRLARYNKKPTI 403
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,855,106
Number of Sequences: 7669
Number of extensions: 53383
Number of successful extensions: 634
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 220499860
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)