TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.29
(685 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular tr... 199 2e-052
gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular tr... 195 4e-051
gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular tr... 160 2e-040
gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular tr... 138 5e-034
gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular tr... 123 2e-029
>gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 521
Score = 199 bits (430), Expect = 2e-052
Identities = 87/95 (91%), Positives = 91/95 (95%)
Frame = +1 / +2
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSRE 435
VETYKIYIFKVLKQVHPDIG+SSKAM IMNSFINDIFEKLA ES++LARYNKKPTITSRE
Sbjct: 209 VETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 388
Query: 436 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*DPC 540
IQT+VRLVLPGELAKHAVSEGTKAVTKFTSS* C
Sbjct: 389 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*FCC 493
Score = 105 bits (224), Expect = 6e-024
Identities = 54/89 (60%), Positives = 61/89 (68%)
Frame = -3 / -3
Query: 521 VNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLMKEF 342
VN VTALVPSETACLA+SPGRT T V IS EV+VGF SK+SLMK F
Sbjct: 474 VNLVTALVPSETACLANSPGRTNLTAV*ISLEVIVGFLLYLAKRDDS*ASFSKISLMKLF 295
Query: 341 MMDMALDEMPMSGCTCFSTLKM*IL*VST 255
++ +AL E P+SG TCF TLKM IL VST
Sbjct: 294 IIPIALLETPISG*TCFRTLKMYILYVST 208
>gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 566
Score = 195 bits (421), Expect = 4e-051
Identities = 86/92 (93%), Positives = 89/92 (96%)
Frame = +1 / +1
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSRE 435
VETYKIYIFK LKQVHPDIGISSKAM IMNSFINDIFEKLA ES++LARYNKKPTITSRE
Sbjct: 247 VETYKIYIFKGLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 426
Query: 436 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 531
IQT+VRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 427 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS* 522
Score = 121 bits (259), Expect = 9e-029
Identities = 61/89 (68%), Positives = 65/89 (73%)
Frame = -3 / -1
Query: 521 VNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLMKEF 342
VN VTALVPSETACLASSPGRTRRT V ISREVMVGF SK+SLMK F
Sbjct: 512 VNLVTALVPSETACLASSPGRTRRTAV*ISREVMVGFLLYLASLEDS*ASFSKISLMKLF 333
Query: 341 MMDMALDEMPMSGCTCFSTLKM*IL*VST 255
M+ +AL E+PMSG TC LKM*IL*VST
Sbjct: 332 MIPIALLEIPMSG*TCLRPLKM*IL*VST 246
>gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 500
Score = 160 bits (344), Expect = 2e-040
Identities = 69/74 (93%), Positives = 72/74 (97%)
Frame = +1 / +2
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSRE 435
VETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIFEKLA ES++LARYNKKPTITSRE
Sbjct: 263 VETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 442
Query: 436 IQTSVRLVLPGELA 477
IQT+VRLVLPGELA
Sbjct: 443 IQTAVRLVLPGELA 484
>gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 363
Score = 138 bits (297), Expect = 5e-034
Identities = 61/65 (93%), Positives = 64/65 (98%)
Frame = +1 / +2
Query: 337 IMNSFINDIFEKLAAESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTK 516
IMNSFINDIFEKLA ES++LARYNKKPTITSREIQT+VRLVLPGELAKHAVSEGTKAVTK
Sbjct: 8 IMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTK 187
Query: 517 FTSS* 531
FTSS*
Sbjct: 188 FTSS* 202
>gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 403
Score = 123 bits (263), Expect = 2e-029
Identities = 52/56 (92%), Positives = 54/56 (96%)
Frame = +1 / +2
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTI 423
VETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIFEKLA ES++LARYNKKPTI
Sbjct: 236 VETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTI 403
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 2,384,964
Number of Sequences: 7669
Number of extensions: 17725
Number of successful extensions: 307
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 171396950
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)