TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.280
(3445 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRN... 1388 0.0
gb|DQ122788.1| Medicago sativa clone B11 sucrose synthase m... 232 1e-080
gb|CO515699.1|CO515699 s13dSG60G0600052_419547 Glandular tr... 285 2e-077
gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular tr... 204 8e-074
gb|CO517054.1|CO517054 s13dSG31G0500037_468654 Glandular tr... 218 3e-057
gb|DQ122842.1| Medicago sativa clone MF8 sucrose synthase m... 138 4e-046
gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate sy... 65 6e-024
>gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRNA, complete cds
Length = 2760
Score = 1388 bits (3024), Expect(2) = 0.0
Identities = 562/741 (75%), Positives = 635/741 (85%)
Frame = +2 / +2
Query: 278 FEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNXXXXXXXXXXXXXXXAFKEQLVDGQ 457
F ++LR+ QEAIVLPPWVALA+RPRPG+W+Y+RVN FKE+LVDG
Sbjct: 260 FGEVLRSTQEAIVLPPWVALAVRPRPGIWEYLRVNVHALVVENLQPAEFLKFKEELVDGS 439
Query: 458 SNSNFVLELDFEPFNASFPRPSMSKSIGNGVQFLNRHLSSKLFQDKESLYPLLNFLKAHN 637
+N NFVLELDFEPF ASFPRP+++KSIGNGV FLNRHLS+KLF DKESL+PLL FL+ H+
Sbjct: 440 ANGNFVLELDFEPFTASFPRPTLNKSIGNGVHFLNRHLSAKLFHDKESLHPLLEFLRLHS 619
Query: 638 YKGTTMMLNDRIQSLRGLQSSLRKAEEYLLSVPQDTPYSEFNHRFQELGLEKGWGDTAKR 817
YKG T+MLNDRIQ+ LQ LRKAEEYL ++ +TPYSEF HRFQE+GLE+GWGDTA+R
Sbjct: 620 YKGKTLMLNDRIQNPDSLQHVLRKAEEYLSTIDPETPYSEFEHRFQEIGLERGWGDTAER 799
Query: 818 VXXXXXXXXXXXEAPDPANLEKFLGTIPMMFNVVILSPHGYFAQSNVLGYPDTGGQVVYI 997
V EAPDP LE FL IPM+FNVVILSPHGYFAQ +VLGYPDTGGQVVYI
Sbjct: 800 VLESIQLLLDLLEAPDPCTLESFLDRIPMVFNVVILSPHGYFAQDDVLGYPDTGGQVVYI 979
Query: 998 LDQVRALENEMLLRIKQQGLDITPKILIVTRLLPDAAGTTCGQRLEKVIGTEHTDIIRVP 1177
LDQVRALE+EML RIK+QGLDI P+ILI+TRLLPDA GTTCGQRLEKV GTEH I+RVP
Sbjct: 980 LDQVRALESEMLSRIKKQGLDIIPRILIITRLLPDAVGTTCGQRLEKVYGTEHCHILRVP 1159
Query: 1178 FRNENGILRKWISRFDVWPYLETYTEDVSSEIMKEMQAKPDLIIGNYSDGNLVATLLAHK 1357
FR+E GI+RKWISRF+VWPYLETYTEDV+ E+ KE+Q+KPDLI+GNYSDGN+VA+LLAHK
Sbjct: 1160 FRDEKGIVRKWISRFEVWPYLETYTEDVAHELAKELQSKPDLIVGNYSDGNIVASLLAHK 1339
Query: 1358 LGVTQCTIAHALEKTKYPNSDIYLDKFDSQYHFSCQFTADLIAMNHTDFIITSTFQEIAG 1537
LGVTQCTIAHALEKTKYP SDIY KF+ +YHFSCQFTADL AMNHTDFIITSTFQEIAG
Sbjct: 1340 LGVTQCTIAHALEKTKYPESDIYWKKFEEKYHFSCQFTADLFAMNHTDFIITSTFQEIAG 1519
Query: 1538 SKDTVGQYESHIAFTLPGLYRVVHGIDVFDPKFNIVSPGADMSVYYPYTETDKRLTAFHP 1717
SKD VGQYESH AFTLPGLYRVVHGIDVFDPKFNIVSPGAD ++Y+PYTET +RLT+F+P
Sbjct: 1520 SKDKVGQYESHTAFTLPGLYRVVHGIDVFDPKFNIVSPGADQTIYFPYTETSRRLTSFYP 1699
Query: 1718 EIEELIYSDVENSEHKFVLKDKKKPIIFSMARLDRVKNMTGLVEMYGKNARLRELANLVI 1897
EIEEL+YS VEN EH VLKD+ KPIIF+MARLDRVKN+TGLVE YGKNA+LREL NLV+
Sbjct: 1700 EIEELLYSSVENEEHICVLKDRNKPIIFTMARLDRVKNITGLVEWYGKNAKLRELVNLVV 1879
Query: 1898 VAGDHGKESKDREEQAEFKKMYSLIDEYKLKGHIRWISAQMNRVRNGELYRYICDTKGAF 2077
VAGD KESKD EE AE KKMY LI+ YKL G RWIS+QMNRVRNGELYR ICDTKGAF
Sbjct: 1880 VAGDRRKESKDLEEIAEMKKMYGLIETYKLNGQFRWISSQMNRVRNGELYRVICDTKGAF 2059
Query: 2078 VQPAFYEAFGLTVIESMTCGLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFF 2257
VQPA YEAFGLTV+E+M GLPT AT +GGPAEIIV G SG HIDPYH D+AAD+LV FF
Sbjct: 2060 VQPAVYEAFGLTVVEAMATGLPTFATLNGGPAEIIVHGKSGFHIDPYHGDRAADLLVEFF 2239
Query: 2258 DKCKADPSYWDKISQGGLQRIYEKYTWKLYSERLMTLTGVYGFWKYVSNLERRETRRYIE 2437
+K KADPS+WDKISQGGLQRI EKYTW +YS+RL+TLTGVYGFWK+VSNL+R E+RRY+E
Sbjct: 2240 EKVKADPSHWDKISQGGLQRIEEKYTWTIYSQRLLTLTGVYGFWKHVSNLDRLESRRYLE 2419
Query: 2438 MFYALKYRSLASQVPLSFD*Y 2500
MFYALKYR LA VPL+ +*Y
Sbjct: 2420 MFYALKYRKLAESVPLAVE*Y 2482
Score = 532 bits (1155), Expect(3) = 0.0
Identities = 260/412 (63%), Positives = 288/412 (69%)
Frame = -2 / -1
Query: 1659 SAPGETMLNLGSKTSMPWTTR*SPGRVNAMWDSYCPTVSLLPAISWNVLVMMKSVWFMAI 1480
SAPG+TMLN GSKTS+P TTR*SPGRV A+ SYCPT+SLLPAISW VLVMMKSVWF+A
Sbjct: 1641 SAPGDTMLNFGSKTSIPCTTR*SPGRVKAV*LSYCPTLSLLPAISWKVLVMMKSVWFIAK 1462
Query: 1479 RSAVNWQEKWYWLSNLSKYMSELGYLVFSKA*AMVH*VTPNLCASRVATRLPSL*LPMIR 1300
RSAVNWQEK Y+ SN + +S+ GYLVFS A*AMVH*VTPNLCA++ AT PSL* P IR
Sbjct: 1461 RSAVNWQEK*YFSSNFFQ*ISDSGYLVFSSA*AMVH*VTPNLCANKEATMFPSL*FPTIR 1282
Query: 1299 SGLACISFIISLETSSVYVSRYGQTSKREIHLRRMPFSFLKGTRMMSVCSVPMTFSSR*P 1120
SGL C S S TSSV VSRYGQTS REIHLR +PFS LKGTR M CSVP TFSSR P
Sbjct: 1281 SGLLCNSLASS*ATSSV*VSRYGQTSNREIHLRTIPFSSLKGTRRMWQCSVP*TFSSRWP 1102
Query: 1119 HVVPAASGNSLVTMRIFGVISRPCCLILRSISFSKART*SRMYTT*PPVSGYPSTLD*AK 940
VVP ASG V MR+ G+IS+PC L+ SIS SKART*S+M TT PPVSGYP T *AK
Sbjct: 1101 QVVPTASGRRRVIMRMRGMISKPCFLMRLSISLSKART*SKM*TTWPPVSGYPKTSS*AK 922
Query: 939 *P*GERITTLNIIGIVPRNFSKLXXXXXXXXXXXXXXXXXXRFAVSPQPFSKPSSWNLWL 760
*P*GER+TTL IGI+ R S++ R AVSPQP SKP SWNL
Sbjct: 921 *P*GERMTTLKTIGILSRKDSRVQGSGASRRSKRSWIDSRTRSAVSPQPLSKPISWNLCS 742
Query: 759 NSE*GVS*GTLSRYSSAFLRDDWRPRRLWILSFNIIVVPL*L*ALRKFSKGYKLSLSWNN 580
NSE GVS + RYSSAFLR R ILSFNI V PL*L*+LR G KLSLSW +
Sbjct: 741 NSEYGVSGSIVLRYSSAFLRTC*RESGF*ILSFNINVFPL*L*SLRNSKSGCKLSLSWKS 562
Query: 579 LDDRCRLRNCTPFPMDFDMEGRGKEAXXXXXXXXXXXXXLDCPSTSCSLNAK 424
L +R RLR CTPFP+D GRGK+A L PSTS SLN +
Sbjct: 561 LAERWRLRKCTPFPIDLLRVGRGKDAVNGSKSNSSTKFPLALPSTSSSLNLR 406
Score = 445 bits (965), Expect(3) = e-177
Identities = 213/496 (42%), Positives = 314/496 (63%)
Frame = -1 / -3
Query: 1741 AVDELLDFRMEGSESLVGFRIRVVNTHVCSRRDNVELGIENIDAMDDTVEPRKSERDVGL 1562
AV +L DFR+EG +S F +R VN + S R NVEL I++I+ + DTVE KS+ V L
Sbjct: 1723 AVKKLFDFRVEGCQSAASFGVREVNGLISSWRYNVELWIKDINTVHDTVESWKSKGSVTL 1544
Query: 1561 VLPHGVLASRDFLECAGDDEIGVVHGNKVSCELAREVVLAVEFVQVYVRVGVFGFLQGMS 1382
+L + +LAS + LE DDEI VVH K+S +LA EV+L EF V + GV L+ MS
Sbjct: 1543 ILSNLILASSNLLEGTSDDEICVVHCEKISGKLAGEVILLFEFFPVNIGFGVLSLLECMS 1364
Query: 1381 DGTLSDSQLVREQSGD*VAIAVVANDKVRLGLHFFHYFTGNILSVCLQVWPDIKTRDPLA 1202
+GTLSD+ + +Q G+ V+I VV+N+++ L F NILSV +WPD K+ DPLA
Sbjct: 1363 NGTLSDT*FMCQQRGNNVSITVVSNNQIWFALQLFGKLMSNILSVSF*IWPDFKS*DPLA 1184
Query: 1201 EDAILISEGNANDVCVLSTNDLLQPLTARSPSSIRQQPGNNEDLRSDIKALLLNPQKHLI 1022
+++LISEGN+ +V +LS+ DL +PL S I ++ +NE+ R DI+AL LN +HL
Sbjct: 1183 NNSLLISEGNSKNVAMLSSIDLFKPLATSSSDCIGEKTSDNENARDDIQALFLNATEHLA 1004
Query: 1021 LQSTDLIQNVHNLTASVRVSKHIGLSEVAMRRKDNNIEHHWYSSKELLQVGRIRGLKKVK 842
LQ T+LIQ+V+NL +S+RVS+ I LS+V MRRKD+NI+ HW S KE L+ R+R L K++
Sbjct: 1003 LQGTNLIQDVNNLASSIRVSQDIILSKVTMRRKDDNIKDHWDSIKERLKGARVRSL*KIQ 824
Query: 841 KQVECVEYTLRSVTPTLLQAKLLEPMVELRVGSVLRNTQ*ILLCLSQG*LETTKALDSVI 662
++++ +E L V+P LQ LLEP+ + V N *IL Q L+ L+SVI
Sbjct: 823 EKLDRLEDALCGVSPASLQTNLLEPVFKF*VWCFRINCA*ILFSFPQNMLKRIGVLNSVI 644
Query: 661 QHHRRALVVMSLEEVQQGVQTLLVLEQLGRQVSVKELHSISDGLRHGRTRXXXXXXXXXX 482
QH +LV + E+ Q+ + TLLV+E+ R++++KE+H+IS+ L RTR
Sbjct: 643 QHQCLSLVAVKSEKFQKWM*TLLVMEEFSRKMAIKEVHTISN*LVESRTRERCSKWFKVQ 464
Query: 481 XXXKVAVGLSIHQLFLECQVLRNTQLLHSQLTYIHPNVIPDTRPWPDSKCNPWGEHNCFL 302
KV++ SI++ F E + L Q+ ++ +IH ++P+T PW +SKCNPW ++ CFL
Sbjct: 463 LKHKVSISTSINKFFFEFEKLSRLQIFDNKSMHIHSQILPNTWPWTNSKCNPWWQNYCFL 284
Query: 301 SSTKNVFKGCILLLVT 254
++N K I L++
Sbjct: 283 CGSQNFTKCTISQLLS 236
Score = 277 bits (600), Expect(3) = 0.0
Identities = 145/247 (58%), Positives = 154/247 (62%)
Frame = -2 / -1
Query: 2499 Y*SKDSGTWLARLRYFRA*NIXXXXXXXXXXXXLTYFQNPYTPVRVISLSE*SFQVYFS* 2320
Y S GT A LRY RA*NI T FQ P TPV V SL E* VYFS
Sbjct: 2481 YYSTARGTDSANLRYLRA*NISR*RRLSRRSRLDTCFQKP*TPVSVRSL*E*IVHVYFSS 2302
Query: 2319 ILCRPPCEILSQ*LGSALHLSKKLTRISAALSLW*GSMCRPDTPSTMISAGPPWQVAIVG 2140
I RPP EILSQ* GSAL SK TR SAA S W*GSM PD P TMISAGPP +VA VG
Sbjct: 2301 IRWRPP*EILSQ*DGSALTFSKNSTRRSAARSPW*GSMWNPDLPWTMISAGPPLRVANVG 2122
Query: 2139 RPHVMDSITVRPNAS*NAGCTNAPLVSQM*RYSSPLRTRFICAEIHRIWPFNLYSSMRLY 1960
P M S TV+P AS AGCT APLVSQ+ RYSSP TRFI EIH WPFNL S+R Y
Sbjct: 2121 NPVAMASTTVKPKASYTAGCTKAPLVSQITRYSSPFLTRFI*EEIHLNWPFNL*VSIRPY 1942
Query: 1959 IFLNSACXXXXXXXLPWSPATITRFASSLRRAFLPYISTRPVMFFTRSRRAIEKMIGFFL 1780
IF SA SPAT TRF +S AFLPY ST PVMFFTRS A+ K+IG
Sbjct: 1941 IFFISAISSKSFDSFLLSPATTTRFTNSRSLAFLPYHSTSPVMFFTRSNLAMVKIIGLLR 1762
Query: 1779 SFSTNLC 1759
SFST++C
Sbjct: 1761 SFSTHMC 1741
Score = 171 bits (367), Expect(3) = e-177
Identities = 72/163 (44%), Positives = 108/163 (66%)
Frame = -1 / -3
Query: 2467 QATVLQGVEHFDVAAGLAPLQVAHVLPEPVHAGQGHQPLGVELPGVLLIDSLQAAL*DLV 2288
Q VL+ +EH +AA L ++V H+LPE + + +PL V P +LL ++L+ L DLV
Sbjct: 2449 QFAVLESIEHLKIAAALKTVEVRHMLPEAIDTSECKKPLRVNCPCILLFNTLETTLRDLV 2270
Query: 2287 PVARICLAFVKEVDQDIRGLVAVVRVNVQARYPVHDDLSRATMAGRDRWQTARHGLDHSQ 2108
PV I +KE +++I V +VR+NV++R+ + +DLSRAT+ WQ++ HGL++ Q
Sbjct: 2269 PVRWISFDLLKEFNKEISSAVTMVRINVESRFAMDNDLSRATIKSCKCWQSSSHGLNNCQ 2090
Query: 2107 AERFVERRLHECALGIANVAVQLPVADAVHLRRDPPDMALQLV 1979
E FV RLHE +LG+ N VQL V+D VHLR +P ++A+QLV
Sbjct: 2089 TESFVHGRLHESSLGVTNHTVQLSVSDTVHLRGNPSELAIQLV 1961
Score = 142 bits (304), Expect(6) = e-107
Identities = 74/175 (42%), Positives = 101/175 (57%)
Frame = +1 / +1
Query: 1366 HSVYHRSCLGENQIPQLGHILGQIRQXXXXXXXXHS*PYCHEPHRFHHHQHIPRNRGKQG 1545
HSVYH SC E+ +P++ ++L +IR+ + * + +EPHRFHHH ++PR+ KQG
Sbjct: 1348 HSVYHCSCTRED*VPRIRYLLEKIRREVSLLLPIYR*SFRNEPHRFHHH*YLPRDCWKQG 1527
Query: 1546 HRGAVRVPHRVHSSWALPCRPWHRCFRSQVQHCLSWSRHECLLPVYGNRQETHCLPS*NR 1725
V H ++SS LPCR + C S+VQHC+S S + LLP++ N L *NR
Sbjct: 1528 *GWTV*ESHCLYSSRTLPCRARY*CL*SKVQHCISRS*SDHLLPLHRN*PPIDILLP*NR 1707
Query: 1726 GAHLQRRRELRAQVRAEGQEEADHLLDGASRPREEHDRPGRDVRQERAPEGAGEP 1890
A LQ E RA + AEG ++A++L G P EEH R VRQE GEP
Sbjct: 1708 RASLQLS*E*RAHMCAEGPQQANYLHHGKVGPCEEHYRTCGVVRQECQAS*VGEP 1872
Score = 134 bits (287), Expect(6) = e-107
Identities = 66/143 (46%), Positives = 83/143 (58%)
Frame = +1 / +1
Query: 901 NDVQCCYPFSSWLLRSVQCAWIP*HWRSGCVHSGSGPCFGE*DASED*AARP*YHSEDPH 1080
N + CC+PFSSWLL S C IP*+WR GC+H GS C GE DA * R YH H
Sbjct: 883 NGL*CCHPFSSWLLCSR*CLGIP*YWRPGCLHLGSSSCLGERDAQSH*ETRLGYHPSHSH 1062
Query: 1081 CYQAVA*CCWDYVRSAAGEGHWY*AHRHHSRSLQK*EWHPPQVDLSF*CLAIPGDIH*GC 1260
+ + C +Y+ A +G W *A H S SLQ+*E + QVDL+ * LAI ++H*GC
Sbjct: 1063 YHSSSPRCSRNYLWPTA*KGLWN*ALPHSSSSLQR*EGNCSQVDLTI*SLAISRNLH*GC 1242
Query: 1261 FQ*NNERNAGQA*PYHWQLQRWQ 1329
* +R A Q W+LQ W+
Sbjct: 1243 CS*ACQRVAKQTRSDCWKLQ*WK 1311
Score = 131 bits (281), Expect(5) = 2e-082
Identities = 74/167 (44%), Positives = 89/167 (53%)
Frame = -3 / -2
Query: 1268 HWKHPQCMSPGMARHQNERSTCGGCHSHF*RERE*CLCAQYQ*PSPAADRT*SQQHQATA 1089
H +HPQC MAR Q RSTC SH *RE E C AQ+ P A * + H+
Sbjct: 1250 HEQHPQCKFLDMARLQIVRSTCEQFPSHL*RELEECGNAQFHRPFQAVGHK*FRLHRGED 1071
Query: 1088 W*Q*GSSE*YQGLAA*SSEASHSPKHGPDPECTQPDRQCQGIQAHWTERSSHEEKG*QH* 909
* * *Y L +* AS SP+H DP C QP Q QGI H E+S+HEEKG*QH*
Sbjct: 1070 E***ECEG*YPSLVS*CD*ASRSPRHELDPRCKQPGLQYQGIPRHHLEQSNHEEKG*QH* 891
Query: 908 TSLV*FQGTSPSWQDQGPQEGQEASGVCRVHASQCHPNPSPSQALGT 768
L +QG + + Q P E G R A +C P+ SP+Q+ GT
Sbjct: 890 RPLGFYQGKTQGCKGQEPLEDPREVG*TRGRALRCLPSLSPNQSPGT 750
Score = 122 bits (260), Expect(4) = 3e-043
Identities = 75/180 (41%), Positives = 88/180 (48%)
Frame = +3 / +3
Query: 750 TRSSTIGSKSLAWRRVGVTLRSVYSTHSTCFLTFLRPLILPTWRSSLELYQ*CSMLLSFL 929
T++ GS+ L WR G T +S S+ S F R L L SL Q ML SFL
Sbjct: 732 TQNLNTGSRRLVWREAGETPQSASSSLSNFSWIF*RLLTLAPLSLSLIESQWSLMLSSFL 911
Query: 930 LMATSLSPMCLDTLTLAVRLCTFWIRSVLWRMRCF*GLSSKALISLRRSSXXXXXXXXXX 1109
LM T L M DTL L RL T WI+ V WR RC L +KA IS S
Sbjct: 912 LMVTLLKMMSWDTLILEARLFTSWIKFVPWRARCSVALRNKAWISSLAFSLSLVFSPMQS 1091
Query: 1110 XXRAVSGWRRSLVLSTQTSFAFPSEMRMASSASGSLVLMSGHTWRHTLRMFPVK**KKCR 1289
+G +RS+ LS T F FPSEMR ASGS L SGH + TLRM + K C+
Sbjct: 1092 ELLVANGLKRSMELSIATFFEFPSEMRRELFASGSHDLKSGHI*KLTLRMLLMSLPKSCK 1271
Score = 95.9 bits (203), Expect(5) = 2e-082
Identities = 55/119 (46%), Positives = 65/119 (54%)
Frame = -3 / -2
Query: 1721 FQDGRQ*VSCRFPYTGSKHSCLLQERQC*TWDRKHRCHGRHGRAQEE*TRCGTRTAPRCP 1542
FQ R + F GSK S L E QC*T D++H+ RHGR EE* +C + T P
Sbjct: 1703 FQGRRMSIGG*FRCKGSKWSDQLLEIQC*TLDQRHQYRARHGRVLEE*RQCDSHTVQPYP 1524
Query: 1541 CFPRFLGMCW***NRCGSWQ*GQL*TGKRSGTGCRICPSICPSWGIWFSPRHERWYTE* 1365
CF + LG ***N CGS + Q * G+RS T RI S G S HE+WYTE*
Sbjct: 1523 CFQQSLGRY****NLCGSLRKDQR*IGRRSDTSLRIFSSKYRIRGT*SSRVHEQWYTE* 1347
Score = 83.1 bits (175), Expect(5) = 2e-082
Identities = 48/111 (43%), Positives = 55/111 (49%)
Frame = -3 / -2
Query: 2297 RSCPSSSDLPCICQRS*PGYPRPCRCGKGQCAGQIPRPR*SQQGHHGRSRSLADRTSWTR 2118
RSCPS D QR G + GK QC QI +*SQQGHH + LA + W +
Sbjct: 2279 RSCPSEMDQL*PSQRIQQGDQQRGHHGKDQCGIQICHGQ*SQQGHH*ELQMLAIQ*PWPQ 2100
Query: 2117 SQSGRTLRRTQAARMRPWYRKCSGTAPRCGRGSSAPRSTGYGPSTCTRR*G 1965
S R LR Q AR PW K GTA R GSS +S G STC + G
Sbjct: 2099 QLSNRKLRTRQVARKLPWCHKSHGTALRF*HGSSERKSI*IGHSTCRSQLG 1947
Score = 76.2 bits (160), Expect(6) = e-107
Identities = 39/85 (45%), Positives = 47/85 (55%)
Frame = +1 / +1
Query: 1966 PHRRVQVEGPYPVDLGADEPRPQRGAVPLHLRYQGRIRAACVLRSVRPDCDRVHDVRSAN 2145
P+ +QVE P +D +DEP +R AVP L +QG RA C +RS R D H AN
Sbjct: 1948 PN*DLQVEWPIQMDFLSDEPCQKRRAVPCDL*HQGSFRATCRVRSFRFDSC*GHGYWIAN 2127
Query: 2146 DRDLPWWPC*DHRGRGIWPAH*PLP 2220
+ WWPC*DH IW H* LP
Sbjct: 2128 ICNS*WWPC*DHCPWQIWIPH*SLP 2202
Score = 71.2 bits (149), Expect(2) = 0.0
Identities = 27/57 (47%), Positives = 44/57 (77%)
Frame = +2 / +2
Query: 98 KLTRLHSLRERLGATFSSHPNELIALFSRYVHQGKGMLQRHQLLAEFDALFDSDKEK 268
+LTR+HSL+ERL T +++ NE++AL SR +GKG+LQ HQ++AEF+ + + ++K
Sbjct: 74 RLTRVHSLKERLDETLTANRNEILALLSRLEAKGKGILQHHQVIAEFEEIPEESRQK 244
Score = 52.4 bits (108), Expect(6) = e-107
Identities = 29/85 (34%), Positives = 39/85 (45%)
Frame = +1 / +1
Query: 301 SGSNCAPPMGCTCYQAKAWCLGLHSXXXXXXXXXXXXXF*VLGIQGTAGGWTIQQQLCA* 480
+GSN MGCTC +KA LG+ *V IQ W +LCA*
Sbjct: 283 TGSNSFATMGCTCCSSKARYLGVSESECACSCCRKFATC*VSQIQRRTC*WKC*WKLCA* 462
Query: 481 A*F*ALQCLLSSSFHVEVHRKWSAI 555
* + C+ SS+ +V+ KW A+
Sbjct: 463 VGL*TIYCIFPSSYSQQVNWKWCAL 537
Score = 51.9 bits (107), Expect(4) = 3e-043
Identities = 39/102 (38%), Positives = 50/102 (49%)
Frame = +3 / +3
Query: 417 LSTWHSRNSWWMDNPTATLCLSLILSPSMPPFLVLPCRSPSEMECNSLTDTCRPSCSRTR 596
LS +S+ + M+ TLCLS L+ + LVL S EM C SL + S TR
Sbjct: 399 LSFSNSKKNLLMEVLMETLCLSWTLNHLLHLSLVLLSTSQLEMVCTSLIAIFLLNSSMTR 578
Query: 597 RVCTPC*TSSRLITTRARR*C*MTESKAFVVSNHP*ERQRSI 722
RV S TR R *C*MTE K ++ N * + +SI
Sbjct: 579 RVYIHFWNFSDFTATRERH*C*MTEFKTPILFNMF*GKLKSI 704
Score = 50.6 bits (104), Expect(6) = e-107
Identities = 31/72 (43%), Positives = 38/72 (52%)
Frame = +1 / +1
Query: 619 LPQGS*LQGHDDDVE*QNPKPSWSPIIPEKGRGVSTECSSRHSLLGVQP*VPRAWLGEGL 798
+ Q S LQG D DVE*QN KP +S E+ VS S +++L + VP W GE L
Sbjct: 601 ISQTSQLQGKDIDVE*QNSKPRFSSTCSEES*RVSKHN*S*NTILRI*TQVPGDWFGERL 780
Query: 799 G*HCEACTRHTP 834
G H A R P
Sbjct: 781 GRHRRARPRVYP 816
Score = 44.6 bits (91), Expect(5) = 2e-082
Identities = 21/45 (46%), Positives = 25/45 (55%)
Frame = -3 / -2
Query: 725 VDTPLPFSGMIGDHEGFGFCHSTSSSCPCSYEP*GSSARGTNSPC 591
+DT S + ++ GF FCHSTS S PCS E * NSPC
Sbjct: 707 LDTLQLSSEHVEENRGFEFCHSTSMSFPCSCEV*EIPKVDVNSPC 573
Score = 41.8 bits (85), Expect(3) = e-177
Identities = 16/38 (42%), Positives = 28/38 (73%)
Frame = -1 / -3
Query: 1906 TGNDHEVRQLPQARVLAVHLDQACHVLHAVETRHREDD 1793
+G++++V QL + +LAV L ++C+VLH V+ H ED+
Sbjct: 1888 SGHNNKVHQLTKLGILAVPLHKSCNVLHTVQPCHGEDN 1775
Score = 40.5 bits (82), Expect(3) = 0.0
Identities = 17/21 (80%), Positives = 17/21 (80%)
Frame = -2 / -1
Query: 366 SQTPGLGLIASATHGGSTIAS 304
SQ PGLG ASATHGG TIAS
Sbjct: 348 SQIPGLGRTASATHGGKTIAS 286
Score = 27.2 bits (53), Expect(4) = 3e-043
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +3 / +3
Query: 1338 PLCSRTSWESLSVPSLMPWRKPNTP 1412
PLC + SLSVP LM R+ +TP
Sbjct: 1320 PLCWHIN*VSLSVPLLMHSRRLSTP 1394
Score = 26.7 bits (52), Expect(4) = 3e-043
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +3 / +3
Query: 303 RKQLCSPHGLHLLSGQGLVSG 365
RKQ HGLHLL QG V G
Sbjct: 285 RKQ*FCHHGLHLLFVQGQVFG 347
Score = 26.3 bits (51), Expect(6) = e-107
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = +1 / +1
Query: 2326 EVHLEALLREADDPDRRVRVLEVREQPGEARDPPLHRNVLRPEVP 2460
EV+++ LL EA + +LE P + +PPL +VL +VP
Sbjct: 2308 EVYMDNLLSEASYTHWCLWLLEACV*PRPS*EPPLS*DVLCSQVP 2442
Score = 23.5 bits (45), Expect(5) = 2e-082
Identities = 10/25 (40%), Positives = 13/25 (52%)
Frame = -3 / -2
Query: 1904 RQRSRGSPAPSGARSCRTSRPGLSC 1830
R + +GSP SCRT+ L C
Sbjct: 1886 RPQQQGSPTHEAWHSCRTTPQVL*C 1812
>gb|DQ122788.1| Medicago sativa clone B11 sucrose synthase mRNA, partial cds
Length = 536
Score = 232 bits (501), Expect(2) = 1e-080
Identities = 88/125 (70%), Positives = 104/125 (83%)
Frame = +2 / +2
Query: 1916 KESKDREEQAEFKKMYSLIDEYKLKGHIRWISAQMNRVRNGELYRYICDTKGAFVQPAFY 2095
K+S DREE AE +KM+ L+ +Y L G RW++AQ NR RNGELYRYI DTKGAFVQPAFY
Sbjct: 161 KKSSDREEIAEIEKMHDLMKQYNLNGEFRWLTAQTNRARNGELYRYIADTKGAFVQPAFY 340
Query: 2096 EAFGLTVIESMTCGLPTIATCHGGPAEIIVDGVSGLHIDPYHSDKAADILVNFFDKCKAD 2275
EAFGLTV+E+MTCGLPT ATCHGGPAEII GVSG HIDPYH DKA+++L+ FF K K D
Sbjct: 341 EAFGLTVVEAMTCGLPTFATCHGGPAEIIEHGVSGFHIDPYHPDKASELLLEFFQKSKED 520
Query: 2276 PSYWD 2290
P++W+
Sbjct: 521 PNHWE 535
Score = 133 bits (285), Expect(2) = 1e-041
Identities = 70/116 (60%), Positives = 75/116 (64%)
Frame = -2 / -3
Query: 2289 SQ*LGSALHLSKKLTRISAALSLW*GSMCRPDTPSTMISAGPPWQVAIVGRPHVMDSITV 2110
SQ GS+L K + S ALS *GS+ PDTP +MISAGPPWQVA VG P VM S TV
Sbjct: 534 SQWFGSSLLFWKNSSNSSEALSG**GSI*NPDTPCSMISAGPPWQVANVGSPQVMASTTV 355
Query: 2109 RPNAS*NAGCTNAPLVSQM*RYSSPLRTRFICAEIHRIWPFNLYSSMRLYIFLNSA 1942
P AS* AG T AP VS M*RYSSPLR F+ A HR PF LY MR IF SA
Sbjct: 354 SPKAS*KAG*TKAPFVSAM*RYSSPLRALFV*AVSHRNSPFKLYCFMRSCIFSISA 187
Score = 84.9 bits (179), Expect(2) = 1e-080
Identities = 35/45 (77%), Positives = 40/45 (88%)
Frame = +2 / +2
Query: 1772 LKDKKKPIIFSMARLDRVKNMTGLVEMYGKNARLRELANLVIVAG 1906
L D+ KPIIFSMARLDRVKN+TGLVE Y KN++LREL NLV+VAG
Sbjct: 14 LADRSKPIIFSMARLDRVKNITGLVESYAKNSKLRELVNLVVVAG 148
Score = 53.3 bits (110), Expect(2) = 1e-041
Identities = 24/40 (60%), Positives = 28/40 (70%)
Frame = -2 / -3
Query: 1905 PATITRFASSLRRAFLPYISTRPVMFFTRSRRAIEKMIGF 1786
PAT TR SSL FL +ST+PVMF T S A+EK+IGF
Sbjct: 147 PATTTRLTSSLNLLFLA*LSTKPVMFLTLSSLAMEKIIGF 28
>gb|CO515699.1|CO515699 s13dSG60G0600052_419547 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 449
Score = 285 bits (617), Expect = 2e-077
Identities = 117/148 (79%), Positives = 129/148 (87%)
Frame = +2 / +3
Query: 1697 RLTAFHPEIEELIYSDVENSEHKFVLKDKKKPIIFSMARLDRVKNMTGLVEMYGKNARLR 1876
RL +FHPEIEEL+YS VEN EH VLKD+ KPIIF+MARLDRVKN+TGLVE YGKNARLR
Sbjct: 6 RLKSFHPEIEELLYSSVENEEHICVLKDRSKPIIFTMARLDRVKNITGLVEWYGKNARLR 185
Query: 1877 ELANLVIVAGDHGKESKDREEQAEFKKMYSLIDEYKLKGHIRWISAQMNRVRNGELYRYI 2056
EL NLV+VAGD KESKD EE+AE KKMY LI+ YKL G RWIS+QMNR+RNGELYR I
Sbjct: 186 ELVNLVVVAGDRRKESKDLEEKAEMKKMYGLIETYKLNGQFRWISSQMNRIRNGELYRVI 365
Query: 2057 CDTKGAFVQPAFYEAFGLTVIESMTCGL 2140
CDTKGAFVQPA YEAFGLTV+E+MTCGL
Sbjct: 366 CDTKGAFVQPAIYEAFGLTVVEAMTCGL 449
Score = 173 bits (372), Expect = 1e-043
Identities = 82/142 (57%), Positives = 89/142 (62%)
Frame = -2 / -2
Query: 2136 PHVMDSITVRPNAS*NAGCTNAPLVSQM*RYSSPLRTRFICAEIHRIWPFNLYSSMRLYI 1957
P V+ S TV+P AS* AGCT AP VSQM R SSP R RFIC EIH WPFNL S+R YI
Sbjct: 445 PQVIASTTVKPKAS*IAGCTKAPFVSQMTR*SSPFRIRFICEEIHLNWPFNL*VSIRPYI 266
Query: 1956 FLNSACXXXXXXXLPWSPATITRFASSLRRAFLPYISTRPVMFFTRSRRAIEKMIGFFLS 1777
F SA SPAT TRF SSLRRAFLPY ST PVMFFTRS AI K+IG S
Sbjct: 265 FFISAFSSKSLDSFLLSPATTTRFTSSLRRAFLPYHSTSPVMFFTRSSLAIVKIIGLLRS 86
Query: 1776 FSTNLCXXXXXXXXXXXXXSGW 1711
F+T++C SGW
Sbjct: 85 FNTHMCSSFSTEL*RSSSISGW 20
>gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 515
Score = 204 bits (440), Expect(2) = 8e-074
Identities = 80/105 (76%), Positives = 88/105 (83%)
Frame = +2 / +1
Query: 1916 KESKDREEQAEFKKMYSLIDEYKLKGHIRWISAQMNRVRNGELYRYICDTKGAFVQPAFY 2095
K+S DREE AE +KM+ L+ +Y L G RWI+AQ NR RNGELYRYI DTKGAFVQPAFY
Sbjct: 199 KKSSDREEIAEIEKMHDLMKQYNLNGEFRWITAQTNRARNGELYRYIADTKGAFVQPAFY 378
Query: 2096 EAFGLTVIESMTCGLPTIATCHGGPAEIIVDGVSGLHIDPYHSDK 2230
EAFGLTV+E+MTCGLPT ATCHGGPAEII GVSG HIDPYH DK
Sbjct: 379 EAFGLTVVEAMTCGLPTFATCHGGPAEIIEHGVSGFHIDPYHPDK 513
Score = 127 bits (272), Expect(2) = 4e-039
Identities = 64/97 (65%), Positives = 67/97 (69%)
Frame = -2 / -1
Query: 2232 ALSLW*GSMCRPDTPSTMISAGPPWQVAIVGRPHVMDSITVRPNAS*NAGCTNAPLVSQM 2053
ALS *GS+ PDTP +MISAGPPWQVA VG P VM S TV P AS* AG T AP VS M
Sbjct: 515 ALSG**GSI*NPDTPCSMISAGPPWQVANVGSPQVMASTTVSPKAS*KAG*TKAPFVSAM 336
Query: 2052 *RYSSPLRTRFICAEIHRIWPFNLYSSMRLYIFLNSA 1942
*RYSSPLR F+ A IHR PF LY MR IF SA
Sbjct: 335 *RYSSPLRALFV*AVIHRNSPFKLYCFMRSCIFSISA 225
Score = 90.0 bits (190), Expect(2) = 8e-074
Identities = 38/61 (62%), Positives = 47/61 (77%)
Frame = +2 / +1
Query: 1724 EELIYSDVENSEHKFVLKDKKKPIIFSMARLDRVKNMTGLVEMYGKNARLRELANLVIVA 1903
E+L+Y + E L D+ KPIIFSMARLDRVKN+TGLVE Y KN++LREL NL++VA
Sbjct: 4 EKLLYDTEQTDECIGSLADRSKPIIFSMARLDRVKNITGLVESYAKNSKLRELVNLIVVA 183
Query: 1904 G 1906
G
Sbjct: 184 G 186
Score = 51.0 bits (105), Expect(2) = 4e-039
Identities = 25/45 (55%), Positives = 29/45 (64%)
Frame = -2 / -1
Query: 1905 PATITRFASSLRRAFLPYISTRPVMFFTRSRRAIEKMIGFFLSFS 1771
PAT R SSL FL +ST+PVMF T S A+EK+IGF S S
Sbjct: 185 PATTIRLTSSLNLLFLA*LSTKPVMFLTLSSLAMEKIIGFDRSAS 51
>gb|CO517054.1|CO517054 s13dSG31G0500037_468654 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 374
Score = 218 bits (471), Expect = 3e-057
Identities = 93/123 (75%), Positives = 98/123 (79%)
Frame = +2 / +1
Query: 809 AKRVXXXXXXXXXXXEAPDPANLEKFLGTIPMMFNVVILSPHGYFAQSNVLGYPDTGGQV 988
A+RV EAPDP LEKFLG IPM FN VILSPHGYFAQ N LGYPDTGGQV
Sbjct: 4 AERVLEMIQLLLDLLEAPDPFTLEKFLGRIPMAFNAVILSPHGYFAQDNALGYPDTGGQV 183
Query: 989 VYILDQVRALENEMLLRIKQQGLDITPKILIVTRLLPDAAGTTCGQRLEKVIGTEHTDII 1168
VYILDQVRALENEML RIKQQGLDI P+ILI+TRLLPDA GTTCG+RLEKV TEH I+
Sbjct: 184 VYILDQVRALENEMLRRIKQQGLDIKPRILIITRLLPDAVGTTCGERLEKVYDTEHCHIL 363
Query: 1169 RVP 1177
RVP
Sbjct: 364 RVP 372
Score = 150 bits (322), Expect = 1e-036
Identities = 68/102 (66%), Positives = 76/102 (74%)
Frame = -2 / -1
Query: 1176 GTRMMSVCSVPMTFSSR*PHVVPAASGNSLVTMRIFGVISRPCCLILRSISFSKART*SR 997
GTR M CSV TFS R P VVP ASG V MR+ G+IS+PCCLI SISFSKART S+
Sbjct: 371 GTRRMWQCSVSYTFSRRSPQVVPTASGRRRVIMRMRGLISKPCCLIRLSISFSKARTWSK 192
Query: 996 MYTT*PPVSGYPSTLD*AK*P*GERITTLNIIGIVPRNFSKL 871
MYTT PPVSGYP L AK*P GER+T L IGI+PRNFS++
Sbjct: 191 MYTTCPPVSGYPKALSWAK*PCGERMTALKAIGILPRNFSRV 66
Score = 116 bits (247), Expect = 2e-026
Identities = 53/121 (43%), Positives = 83/121 (68%)
Frame = -1 / -3
Query: 1174 NANDVCVLSTNDLLQPLTARSPSSIRQQPGNNEDLRSDIKALLLNPQKHLILQSTDLIQN 995
N+ +V + L+ T S IR++ G+NE+ R DI+ALLL+ +HL+LQ ++L+QN
Sbjct: 369 NSKNVAMFGIVYFLKTFTTSSSHCIREKTGDNENARLDIQALLLDTSEHLVLQGSNLVQN 190
Query: 994 VHNLTASVRVSKHIGLSEVAMRRKDNNIEHHWYSSKELLQVGRIRGLKKVKKQVECVEYT 815
VHNL+ +RVS+ I L EV M RKD++I+ H SSKE L+ RIR L+K++++++ + T
Sbjct: 189 VHNLSTGIRVSQGIILGEVTMWRKDDSIKGHRDSSKEFLEGERIRCLEKIQQKLDHFKDT 10
Query: 814 L 812
L
Sbjct: 9 L 7
>gb|DQ122842.1| Medicago sativa clone MF8 sucrose synthase mRNA, partial cds
Length = 652
Score = 138 bits (297), Expect(2) = 4e-046
Identities = 54/89 (60%), Positives = 68/89 (76%)
Frame = +2 / +2
Query: 278 FEDILRAAQEAIVLPPWVALAIRPRPGVWDYIRVNXXXXXXXXXXXXXXXAFKEQLVDGQ 457
F ++LR+ QEA+VLPP+VALA+RPRPGVW+Y+RV+ FKE+LV+G
Sbjct: 386 FGEVLRSTQEAVVLPPFVALAVRPRPGVWEYLRVDVHSLVVDELRAAEYLKFKEELVEGS 565
Query: 458 SNSNFVLELDFEPFNASFPRPSMSKSIGN 544
SN NFVLELDFEPFNA FPRP+++KSIGN
Sbjct: 566 SNGNFVLELDFEPFNAPFPRPTLNKSIGN 652
Score = 62.9 bits (131), Expect(2) = 4e-046
Identities = 26/58 (44%), Positives = 40/58 (68%)
Frame = +2 / +2
Query: 95 AKLTRLHSLRERLGATFSSHPNELIALFSRYVHQGKGMLQRHQLLAEFDALFDSDKEK 268
A LTR SLRER + ++H E++AL SR +GKG+LQ HQ++AEF+ + + ++K
Sbjct: 197 ASLTRSTSLRERFDGSLTAHRIEILALLSRIEAKGKGILQHHQVIAEFEEIPEEKRQK 370
>gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate synthase mRNA, complete cds
Length = 3579
Score = 65.2 bits (136), Expect(6) = 6e-024
Identities = 26/47 (55%), Positives = 32/47 (68%)
Frame = +2 / +2
Query: 2039 ELYRYICDTKGAFVQPAFYEAFGLTVIESMTCGLPTIATCHGGPAEI 2179
E+YR TKG FV PA E FGLT+IE+ GLP +AT +GGP +I
Sbjct: 1817 EIYRLAAKTKGVFVNPAIIEPFGLTLIEAAAYGLPMVATKNGGPVDI 1957
Score = 49.6 bits (102), Expect(6) = 6e-024
Identities = 20/42 (47%), Positives = 31/42 (73%)
Frame = +2 / +2
Query: 1784 KKPIIFSMARLDRVKNMTGLVEMYGKNARLRELANLVIVAGD 1909
+KP+I ++AR D KN+T LV+ +G+ LRELANL ++ G+
Sbjct: 1562 RKPVILALARPDPKKNITTLVKAFGECRPLRELANLTLIMGN 1687
Score = 27.6 bits (54), Expect(6) = 6e-024
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2 / +2
Query: 1430 DKFDSQYHFSCQFTADLIAMNHTDFIITSTFQEI 1531
D+ ++ Y + A+ +A++ ++ +ITST QE+
Sbjct: 1208 DEINTTYKIMRRIEAEELALDGSEIVITSTRQEV 1309
Score = 27.6 bits (54), Expect(6) = 6e-024
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +2 / +2
Query: 971 DTGGQVVYILDQVRAL 1018
DTGGQV Y+++ RAL
Sbjct: 707 DTGGQVKYVVELARAL 754
Score = 25.4 bits (49), Expect(6) = 6e-024
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +2 / +2
Query: 1295 PDLIIGNYSDGNLVATLLAHKLGVTQCTIAHALEKTK 1405
P I G+Y+D A LL+ L V H+L + K
Sbjct: 1061 PVAIHGHYADAGDSAALLSGALNVPMLFTGHSLGRDK 1171
Score = 25.4 bits (49), Expect(6) = 6e-024
Identities = 8/33 (24%), Positives = 18/33 (54%)
Frame = +2 / +2
Query: 1193 GILRKWISRFDVWPYLETYTEDVSSEIMKEMQA 1291
G K+I + ++WPY+ + + I++ +A
Sbjct: 926 GPRNKYIPKEELWPYIPEFVDGAIGHILQMSKA 1024
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 28,946,968
Number of Sequences: 7669
Number of extensions: 435437
Number of successful extensions: 2686
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 46
effective length of database: 895,794
effective search space used: 986269194
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)