TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS19f07.yg.2.1
(626 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF621467.2|BF621467 HVSMEa0011B10f Hordeum vulgare seedl... 266 9e-071
gb|BQ469016.1|BQ469016 HM03B24r HM Hordeum vulgare subsp. v... 264 2e-070
gb|BU995729.1|BU995729 HM11C24r HM Hordeum vulgare subsp. v... 226 1e-064
gb|CA009554.1|CA009554 HU14H09r HU Hordeum vulgare subsp. v... 173 1e-061
gb|BQ472196.1|BQ472196 HV04L02r HV Hordeum vulgare subsp. v... 163 1e-058
gb|CA032804.1|CA032804 HX14E02r HX Hordeum vulgare subsp. v... 88 1e-026
gb|BU987774.1|BU987774 HF15L16r HF Hordeum vulgare subsp. v... 88 1e-026
gb|CA022161.1|CA022161 HZ42F09r HZ Hordeum vulgare subsp. v... 118 2e-026
gb|AV934271.1|AV934271 AV934271 K. Sato unpublished cDNA li... 83 3e-026
gb|BJ472357.1|BJ472357 BJ472357 K. Sato unpublished cDNA li... 77 1e-024
>gb|BF621467.2|BF621467 HVSMEa0011B10f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011B10f, mRNA sequence
Length = 815
Score = 266 bits (575), Expect = 9e-071
Identities = 105/107 (98%), Positives = 106/107 (99%)
Frame = -1 / +2
Query: 506 EFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNL 327
EFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDP+RWKNSHWRNL
Sbjct: 5 EFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPVRWKNSHWRNL 184
Query: 326 QVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMP 186
QVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQ GMP
Sbjct: 185 QVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQPGMP 325
Score = 228 bits (492), Expect = 2e-059
Identities = 98/110 (89%), Positives = 102/110 (92%)
Frame = +1 / -3
Query: 178 SSSGIPACLGSFGLKNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHR 357
S+ GIP CLGSFGLKNGGG I* GVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHR
Sbjct: 333 SAPGIPGCLGSFGLKNGGGQI*KGVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHR 154
Query: 358 SGSKSPMPVIVPMYLLTPESSVSKSILNRIPRDTCVYRALLYFAKGMTNS 507
+GSKSP+PVIVPMYL TPESSVS SILN IPR+TCVYRALLY AKGMTNS
Sbjct: 153 TGSKSPIPVIVPMYLRTPESSVSNSILNLIPRETCVYRALLYLAKGMTNS 4
Score = 182 bits (392), Expect = 2e-045
Identities = 73/107 (68%), Positives = 79/107 (73%)
Frame = +3 / -2
Query: 186 WHSCLLRKLWPEKWWWAYIKWCSNWFNLPYRNTGAPLRCSRFIPTNLKVSPMRILPSQWI 365
WHS LLRK WPEKWWW IK C NWFNLPYRNT APLRCS FIP NLKV PMRI P I
Sbjct: 325 WHSWLLRKFWPEKWWWTNIKRCGNWFNLPYRNTSAPLRCS*FIPANLKVPPMRIFPPHRI 146
Query: 366 *VANACDRAHVSSYP*ILCLKEHSESHSKGHLCIQSLIVFC*RDDKF 506
V N D AHV S P IL L++H E H K +LCIQSL++F *R+DKF
Sbjct: 145 QVTNTRDCAHVPSDPRILSLEQHPEPHPKRNLCIQSLVIFG*RNDKF 5
Score = 168 bits (361), Expect = 3e-041
Identities = 73/109 (66%), Positives = 82/109 (75%)
Frame = -2 / +3
Query: 508 QNLSSL*QNTIRLCIHKCPLECDSECSLRQRIQG*EDTWARSQALAT*IHCDGRILIGET 329
+NLS L* N RLCIHK L S C R RI+G E TWA+S+ L T I C G+ILIG T
Sbjct: 3 ENLSFL*PNITRLCIHKFLLG*GSGCCSRLRIRGSEGTWAQSRVLVTWIRCGGKILIGGT 182
Query: 328 FRLVGMNRLHLRGAPVFRYGRLNQLLHHFIYAHHHFSGQSFLSKQECQM 182
FRL GMN+LHLRGA VFRYGRLNQL H FI+ HHHFSGQ+FLS QECQ+
Sbjct: 183 FRLAGMNQLHLRGALVFRYGRLNQLPHLFIFVHHHFSGQNFLSSQECQV 329
Score = 164 bits (353), Expect = 4e-040
Identities = 66/97 (68%), Positives = 80/97 (82%)
Frame = -3 / +1
Query: 507 RICHPFSKIQ*GSVYTSVPWNAIQNAL*DRGFRGKKIHGHDHRHWRLRSTAMEEFSLAKP 328
RICH FS+I GSVYTS W+ +Q+A+ D GF G K+HGH+H +W L S A+E+FSLA+P
Sbjct: 4 RICHSFSQI*QGSVYTSFSWDEVQDAVRD*GFGGPKVHGHNHGYW*LGSGAVEKFSLAEP 183
Query: 327 SGWLG*IDCI*EAHPCFDMGD*TSCYTILYMPTTIFQ 217
SGWLG*I+CI*EAH CFDMGD*TSC+T LY+ TTIFQ
Sbjct: 184 SGWLG*INCI*EAHSCFDMGD*TSCHTFLYLSTTIFQ 294
Score = 127 bits (273), Expect = 4e-029
Identities = 64/96 (66%), Positives = 67/96 (69%)
Frame = +2 / -1
Query: 221 KMVVGIYKMV*QLVQSPISKHGCASQMQSIHPNQPEGFANENSSIAVDLSRQCL*SCPCI 400
KMVV YK V QLVQSPISKH CASQMQ IHP+QPEG ANEN S A D S Q * CPC
Sbjct: 290 KMVVDKYKKVWQLVQSPISKHECASQMQLIHPSQPEGSANENFSTAPDPSHQYP*LCPCT 111
Query: 401 FLPLNPLSQRAF*IAFQGTLVYTEPYCILLKG*QIL 508
F P NP S+ A * + Q LVYTEP I LK *QIL
Sbjct: 110 FGPPNPQSRTAS*TSSQEKLVYTEPCYIWLKE*QIL 3
>gb|BQ469016.1|BQ469016 HM03B24r HM Hordeum vulgare subsp. vulgare cDNA clone HM03B24
5-PRIME, mRNA sequence
Length = 582
Score = 264 bits (572), Expect = 2e-070
Identities = 102/109 (93%), Positives = 106/109 (97%)
Frame = -1 / +3
Query: 365 DPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMP 186
DP+RWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQ GMP
Sbjct: 12 DPVRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQPGMP 191
Query: 185 DDENEVESAFKRAMPWLADDFALKDVQNALFPGLSLVQWMAMQQNPQML 39
DDE+EV+SAFKRAMPWL DDFALKDVQ+ LFPGLSLVQWMAMQQNPQML
Sbjct: 192 DDESEVDSAFKRAMPWLGDDFALKDVQSQLFPGLSLVQWMAMQQNPQML 338
Score = 228 bits (493), Expect = 2e-059
Identities = 99/109 (90%), Positives = 102/109 (93%)
Frame = +1 / -3
Query: 40 SI*GFCCIAIH*TRLRPGNNAFWTSFRAKSSASHGIALLKALSTSFSSSGIPACLGSFGL 219
SI*GFCCIAIH*T+LRPGNN WTSFRAKSS SHG+ALLKALSTS SSSGIP CLGSFGL
Sbjct: 337 SI*GFCCIAIH*TKLRPGNN*LWTSFRAKSSPSHGMALLKALSTSLSSSGIPGCLGSFGL 158
Query: 220 KNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHRSGS 366
KNGGG I* GVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHR+GS
Sbjct: 157 KNGGGQI*KGVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHRTGS 11
Score = 223 bits (481), Expect = 8e-058
Identities = 88/112 (78%), Positives = 93/112 (83%)
Frame = +3 / -2
Query: 18 YSRGSSC*HLRILLHSHPLN*AQAWK*CILDIFQSEVISKPWHRSFESTLNLVFIIWHSC 197
+S G+S *HLRILLHSHPLN*AQAWK* LDIF +VI+KPWH SFEST+NL FIIWHS
Sbjct: 359 HSWGTSW*HLRILLHSHPLN*AQAWK*LTLDIF*GKVITKPWHGSFESTINLAFIIWHSW 180
Query: 198 LLRKLWPEKWWWAYIKWCSNWFNLPYRNTGAPLRCSRFIPTNLKVSPMRILP 353
LLRK WPEKWWW IK C NWFNLPYRNT APLRCS FIP NLKV PMRI P
Sbjct: 179 LLRKFWPEKWWWTNIKRCGNWFNLPYRNTSAPLRCS*FIPANLKVPPMRIFP 24
Score = 199 bits (429), Expect = 1e-050
Identities = 83/105 (79%), Positives = 91/105 (86%)
Frame = -2 / +1
Query: 364 IHCDGRILIGETFRLVGMNRLHLRGAPVFRYGRLNQLLHHFIYAHHHFSGQSFLSKQECQ 185
I C G+ILIG TFRL GMN+LHLRGA VFRYGRLNQL H FI+ HHHFSGQ+FLS QECQ
Sbjct: 13 IRCGGKILIGGTFRLAGMNQLHLRGALVFRYGRLNQLPHLFIFVHHHFSGQNFLSSQECQ 192
Query: 184 MMKTRLRVLSKERCHGLLMTSL*KMSKMHYFQA*A*FNGWLCSKI 50
MMK RL VLSKE CHGL+MT *KMSK++YFQA*A*FNGWLCS+I
Sbjct: 193 MMKARLIVLSKEPCHGLVMTLP*KMSKVNYFQA*A*FNGWLCSRI 327
Score = 174 bits (374), Expect = 5e-043
Identities = 80/115 (69%), Positives = 90/115 (78%)
Frame = -3 / +2
Query: 363 STAMEEFSLAKPSGWLG*IDCI*EAHPCFDMGD*TSCYTILYMPTTIFQXXXXXXXXXXX 184
S A+E+FSLA+PSGWLG*I+CI*EAH CFDMGD*TSC+T LY+ TTIFQ
Sbjct: 14 SGAVEKFSLAEPSGWLG*INCI*EAHSCFDMGD*TSCHTFLYLSTTIFQAKTS*AARNAR 193
Query: 183 **KRG*ECFQKSDAMAC**LRSERCPKCIISRPEPSSMDGYAAKSSDASNCFPCC 19
**KRG* CFQKS AMA **L +RCPK IISRPE SSMDGYAA+SSDA++ P C
Sbjct: 194 **KRG**CFQKSHAMAW**LCPKRCPKSIISRPELSSMDGYAAESSDATSWCPSC 358
Score = 167 bits (360), Expect = 4e-041
Identities = 79/115 (68%), Positives = 83/115 (72%)
Frame = +2 / -1
Query: 20 QQGKQLLASEDFAA*PSIELGSGLEIMHFGHLSERSHQQAMASLF*KHSQPRFHHXXXXX 199
Q G QL+ASED AA*PSIEL SGLEI+ FGHL +SH QAMA LF*KH QPRFHH
Sbjct: 357 QLGHQLVASEDSAA*PSIELSSGLEIIDFGHLLGQSHHQAMAWLF*KHYQPRFHHLAFLA 178
Query: 200 XXXXXXXKMVVGIYKMV*QLVQSPISKHGCASQMQSIHPNQPEGFANENSSIAVD 364
KMVV YK V QLVQSPISKH CASQMQ IHP+QPEG ANEN S A D
Sbjct: 177 A*EVLA*KMVVDKYKKVWQLVQSPISKHECASQMQLIHPSQPEGSANENFSTAPD 13
>gb|BU995729.1|BU995729 HM11C24r HM Hordeum vulgare subsp. vulgare cDNA clone HM11C24
5-PRIME, mRNA sequence
Length = 640
Score = 226 bits (488), Expect(2) = 1e-064
Identities = 89/95 (93%), Positives = 92/95 (96%)
Frame = -1 / +1
Query: 323 VGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMPDDENEVESAFKRAM 144
VGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQ GMPDDE+EV+SAFKRAM
Sbjct: 52 VGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQPGMPDDESEVDSAFKRAM 231
Query: 143 PWLADDFALKDVQNALFPGLSLVQWMAMQQNPQML 39
PWL DDFALKDVQ+ LFPGLSLVQWMAMQQNPQML
Sbjct: 232 PWLGDDFALKDVQSQLFPGLSLVQWMAMQQNPQML 336
Score = 207 bits (447), Expect(2) = 3e-057
Identities = 81/104 (77%), Positives = 87/104 (83%)
Frame = +3 / -2
Query: 18 YSRGSSC*HLRILLHSHPLN*AQAWK*CILDIFQSEVISKPWHRSFESTLNLVFIIWHSC 197
+S G+S *HLRILLHSHPLN*AQAWK* LDIF +VI+KPWH SFEST+NL FIIWHS
Sbjct: 357 HSWGTSW*HLRILLHSHPLN*AQAWK*LTLDIF*GKVITKPWHGSFESTINLAFIIWHSW 178
Query: 198 LLRKLWPEKWWWAYIKWCSNWFNLPYRNTGAPLRCSRFIPTNLK 329
LLRK WPEKWWW IK C NWFNLPYRNT APLRCS FIP NL+
Sbjct: 177 LLRKFWPEKWWWTNIKRCGNWFNLPYRNTSAPLRCS*FIPANLR 46
Score = 199 bits (430), Expect = 9e-051
Identities = 87/96 (90%), Positives = 89/96 (92%)
Frame = +1 / -3
Query: 40 SI*GFCCIAIH*TRLRPGNNAFWTSFRAKSSASHGIALLKALSTSFSSSGIPACLGSFGL 219
SI*GFCCIAIH*T+LRPGNN WTSFRAKSS SHG+ALLKALSTS SSSGIP CLGSFGL
Sbjct: 335 SI*GFCCIAIH*TKLRPGNN*LWTSFRAKSSPSHGMALLKALSTSLSSSGIPGCLGSFGL 156
Query: 220 KNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT* 327
KNGGG I* GVATGSISHIETRVRLSDAVDSSQPT*
Sbjct: 155 KNGGGQI*KGVATGSISHIETRVRLSDAVDSSQPT* 48
Score = 177 bits (382), Expect(2) = 5e-045
Identities = 74/92 (80%), Positives = 81/92 (88%)
Frame = -2 / +2
Query: 325 RLVGMNRLHLRGAPVFRYGRLNQLLHHFIYAHHHFSGQSFLSKQECQMMKTRLRVLSKER 146
RL GMN+LHLRGA VFRYGRLNQL H FI+ HHHFSGQ+FLS QECQMMK RL VLSKE
Sbjct: 50 RLAGMNQLHLRGALVFRYGRLNQLPHLFIFVHHHFSGQNFLSSQECQMMKARLIVLSKEP 229
Query: 145 CHGLLMTSL*KMSKMHYFQA*A*FNGWLCSKI 50
CHGL+MT *KMSK++YFQA*A*FNGWLCS+I
Sbjct: 230 CHGLVMTLP*KMSKVNYFQA*A*FNGWLCSRI 325
Score = 154 bits (331), Expect = 4e-037
Identities = 71/102 (69%), Positives = 78/102 (76%)
Frame = -3 / +3
Query: 324 GWLG*IDCI*EAHPCFDMGD*TSCYTILYMPTTIFQXXXXXXXXXXX**KRG*ECFQKSD 145
GWLG*I+CI*EAH CFDMGD*TSC+T LY+ TTIFQ **KRG* CFQKS
Sbjct: 51 GWLG*INCI*EAHSCFDMGD*TSCHTFLYLSTTIFQAKTS*AARNAR**KRG**CFQKSH 230
Query: 144 AMAC**LRSERCPKCIISRPEPSSMDGYAAKSSDASNCFPCC 19
AMA **L +RCPK IISRPE SSMDGYAA+SSDA++ P C
Sbjct: 231 AMAW**LCPKRCPKSIISRPELSSMDGYAAESSDATSWCPSC 356
Score = 150 bits (322), Expect = 7e-036
Identities = 70/103 (67%), Positives = 75/103 (72%)
Frame = +2 / -1
Query: 20 QQGKQLLASEDFAA*PSIELGSGLEIMHFGHLSERSHQQAMASLF*KHSQPRFHHXXXXX 199
Q G QL+ASED AA*PSIEL SGLEI+ FGHL +SH QAMA LF*KH QPRFHH
Sbjct: 355 QLGHQLVASEDSAA*PSIELSSGLEIIDFGHLLGQSHHQAMAWLF*KHYQPRFHHLAFLA 176
Query: 200 XXXXXXXKMVVGIYKMV*QLVQSPISKHGCASQMQSIHPNQPE 328
KMVV YK V QLVQSPISKH CASQMQ IHP+QP+
Sbjct: 175 A*EVLA*KMVVDKYKKVWQLVQSPISKHECASQMQLIHPSQPK 47
Score = 40.0 bits (81), Expect(2) = 1e-064
Identities = 12/15 (80%), Positives = 15/15 (100%)
Frame = -1 / +2
Query: 365 DPLRWKNSHWRNLQV 321
DP+RWKNSHWRNL++
Sbjct: 11 DPVRWKNSHWRNLRL 55
Score = 34.1 bits (68), Expect(2) = 3e-057
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +1 / -2
Query: 328 RFRQ*EFFHRSGSKSP 375
RFRQ*EFFHR+GS P
Sbjct: 48 RFRQ*EFFHRTGSPRP 1
Score = 23.1 bits (44), Expect(2) = 5e-045
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = -2 / +3
Query: 364 IHCDGRILIGETFRLVG 314
I C G+ILIG T +G
Sbjct: 12 IRCGGKILIGGTLGWLG 62
>gb|CA009554.1|CA009554 HU14H09r HU Hordeum vulgare subsp. vulgare cDNA clone HU14H09
5-PRIME, mRNA sequence
Length = 628
Score = 173 bits (372), Expect(2) = 1e-061
Identities = 66/95 (69%), Positives = 77/95 (81%)
Frame = -1 / +1
Query: 461 QVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTR 282
Q+SLGMRFRM+FETE+SG RRYMGTITGI DLDP+RWKNS WRN+QV WDE+ SERRTR
Sbjct: 322 QLSLGMRFRMMFETEESGTRRYMGTITGISDLDPVRWKNSQWRNIQVAWDEAAPSERRTR 501
Query: 281 VSIWEIEPVATPFYICPPPFFRPKLPKQAGMPDDE 177
VS+W+IEPV PF+I P P F K +Q M DD+
Sbjct: 502 VSLWDIEPVIAPFFIYPTPLFTAKRARQPXMIDDD 606
Score = 116 bits (249), Expect(2) = 1e-033
Identities = 57/93 (61%), Positives = 64/93 (68%)
Frame = +1 / -3
Query: 178 SSSGIPACLGSFGLKNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHR 357
SSS I CL F + NG G + NG TGS+S ET VRLS SS T* R *EFFH
Sbjct: 605 SSSIIXGCLARFAVNNGVG*MKNGAMTGSMSQRETLVRLSLGAASSHAT*ILRH*EFFHL 426
Query: 358 SGSKSPMPVIVPMYLLTPESSVSKSILNRIPRD 456
+GS+S MPVIVPMYLL P+SSVS ILNR+PRD
Sbjct: 425 TGSRSLMPVIVPMYLLVPDSSVSNIILNRMPRD 327
Score = 83.1 bits (175), Expect(2) = 1e-061
Identities = 35/54 (64%), Positives = 38/54 (70%)
Frame = -1 / +1
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
LSSS S M + AA HAAAN+ FTIFYNPRA PSEFVIP AKY KA+Y
Sbjct: 154 LSSSVLSSDSMHIGVLAAAAHAAANNSQFTIFYNPRASPSEFVIPFAKYQKAVY 315
Score = 46.0 bits (94), Expect(2) = 1e-033
Identities = 22/48 (45%), Positives = 25/48 (52%)
Frame = +1 / -3
Query: 481 YFAKGMTNSDGXALGL*KIVNGLLFXXXXXXXXXIXXXMXXXXXXDDD 624
Y AKG+TNS+G ALGL* IVN LLF M DD+
Sbjct: 299 YLAKGITNSEGLALGL*NIVNWLLFAAA*AAAARTPICMLSLDKTDDE 156
>gb|BQ472196.1|BQ472196 HV04L02r HV Hordeum vulgare subsp. vulgare cDNA clone HV04L02
5-PRIME, mRNA sequence
Length = 566
Score = 163 bits (351), Expect(2) = 1e-058
Identities = 61/81 (75%), Positives = 70/81 (86%)
Frame = -1 / +1
Query: 461 QVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTR 282
Q+SLGMRFRM+FETE+SG RRYMGTITGI DLDP+RWKNS WRN+QV WDE+ SERRTR
Sbjct: 322 QLSLGMRFRMMFETEESGTRRYMGTITGISDLDPVRWKNSQWRNIQVAWDEAAPSERRTR 501
Query: 281 VSIWEIEPVATPFYICPPPFF 219
VS+W+IEPV PF+I P P F
Sbjct: 502 VSLWDIEPVIAPFFIYPTPLF 564
Score = 104 bits (221), Expect(2) = 7e-030
Identities = 50/80 (62%), Positives = 57/80 (71%)
Frame = +1 / -1
Query: 217 LKNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHRSGSKSPMPVIVPM 396
+ NG G + NG TGS+S ET VRLS SS T* R *EFFH +GS+S MPVIVPM
Sbjct: 566 VNNGVG*MKNGAMTGSMSQRETLVRLSLGAASSHAT*ILRH*EFFHLTGSRSLMPVIVPM 387
Query: 397 YLLTPESSVSKSILNRIPRD 456
YLL P+SSVS ILNR+PRD
Sbjct: 386 YLLVPDSSVSNIILNRMPRD 327
Score = 83.1 bits (175), Expect(2) = 1e-058
Identities = 35/54 (64%), Positives = 38/54 (70%)
Frame = -1 / +1
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
LSSS S M + AA HAAAN+ FTIFYNPRA PSEFVIP AKY KA+Y
Sbjct: 154 LSSSVLSSDSMHIGVLAAAAHAAANNSQFTIFYNPRASPSEFVIPFAKYQKAVY 315
Score = 46.0 bits (94), Expect(2) = 7e-030
Identities = 22/48 (45%), Positives = 25/48 (52%)
Frame = +1 / -1
Query: 481 YFAKGMTNSDGXALGL*KIVNGLLFXXXXXXXXXIXXXMXXXXXXDDD 624
Y AKG+TNS+G ALGL* IVN LLF M DD+
Sbjct: 299 YLAKGITNSEGLALGL*NIVNWLLFAAA*AAAARTPICMLSLDKTDDE 156
>gb|CA032804.1|CA032804 HX14E02r HX Hordeum vulgare subsp. vulgare cDNA clone HX14E02
5-PRIME, mRNA sequence
Length = 655
Score = 87.7 bits (185), Expect(2) = 1e-026
Identities = 37/88 (42%), Positives = 51/88 (57%)
Frame = -1 / +3
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S M + A HA FT++Y PR PSEF+IP +Y +++ S+GMR
Sbjct: 63 SSVISSHSMHLGVLATAWHAINTKSMFTVYYKPRTSPSEFIIPYDQYMESVKNNYSIGMR 242
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPL 357
FRM FE E++ +R+ GTI G +LD L
Sbjct: 243 FRMRFEGEEAPEQRFTGTIVGSENLDQL 326
Score = 51.9 bits (107), Expect(2) = 1e-026
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = -1 / +3
Query: 353 WKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATP 246
W S+WR+L+V WDE + R RVS W+IEP ++P
Sbjct: 327 WPESNWRSLKVRWDEPSTIPRPDRVSPWKIEPASSP 434
>gb|BU987774.1|BU987774 HF15L16r HF Hordeum vulgare subsp. vulgare cDNA clone HF15L16
5-PRIME, mRNA sequence
Length = 591
Score = 87.7 bits (185), Expect(2) = 1e-026
Identities = 37/88 (42%), Positives = 51/88 (57%)
Frame = -1 / +1
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S M + A HA FT++Y PR PSEF+IP +Y +++ S+GMR
Sbjct: 64 SSVISSHSMHLGVLATAWHAINTKSMFTVYYKPRTSPSEFIIPYDQYMESVKNNYSIGMR 243
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPL 357
FRM FE E++ +R+ GTI G +LD L
Sbjct: 244 FRMRFEGEEAPEQRFTGTIVGSENLDQL 327
Score = 51.9 bits (107), Expect(2) = 1e-026
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = -1 / +1
Query: 353 WKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATP 246
W S+WR+L+V WDE + R RVS W+IEP ++P
Sbjct: 328 WPESNWRSLKVRWDEPSTIPRPDRVSPWKIEPASSP 435
>gb|CA022161.1|CA022161 HZ42F09r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42F09
5-PRIME, mRNA sequence
Length = 576
Score = 118 bits (253), Expect = 2e-026
Identities = 44/63 (69%), Positives = 54/63 (85%)
Frame = -1 / +1
Query: 437 RMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIEP 258
RMLFETE+S VRRYMGTIT + D DP+RW +S+WR+++VGWDESTA ER RVS+WEIEP
Sbjct: 1 RMLFETEESSVRRYMGTITEVSDADPVRWASSYWRSVKVGWDESTAGERPPRVSLWEIEP 180
Query: 257 VAT 249
+ T
Sbjct: 181 LTT 189
>gb|AV934271.1|AV934271 AV934271 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11l09 5', mRNA sequence
Length = 582
Score = 82.6 bits (174), Expect(2) = 3e-026
Identities = 35/90 (38%), Positives = 51/90 (56%)
Frame = -1 / +3
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLG 447
++SS S M + A HA FT++Y PR SEF+IP KY +++ S+G
Sbjct: 174 IASSVISSHSMHLGVLATAWHAINTKTMFTVYYKPRTSRSEFIIPYDKYTESVKNIYSIG 353
Query: 446 MRFRMLFETEDSGVRRYMGTITGIGDLDPL 357
RF+M FE E++ +R+ GTI G +LD L
Sbjct: 354 TRFKMRFEGEEAPEQRFTGTIVGSDNLDQL 443
Score = 55.1 bits (114), Expect(2) = 3e-026
Identities = 20/36 (55%), Positives = 25/36 (69%)
Frame = -1 / +3
Query: 353 WKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATP 246
W S WR+L+V WDES+ R RVS WEIEP ++P
Sbjct: 444 WPESSWRSLKVRWDESSTIPRPDRVSPWEIEPASSP 551
>gb|BJ472357.1|BJ472357 BJ472357 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal33n21 5', mRNA sequence
Length = 684
Score = 77.1 bits (162), Expect(2) = 1e-024
Identities = 32/80 (40%), Positives = 46/80 (57%)
Frame = -1 / +1
Query: 596 MXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMRFRMLFETE 417
M + A HA FT++Y PR SEF+IP KY +++ S+G RF+M FE E
Sbjct: 31 MHLGVLATAWHAINTKTMFTVYYKPRTSRSEFIIPYDKYTESVKNIYSIGTRFKMRFEGE 210
Query: 416 DSGVRRYMGTITGIGDLDPL 357
++ +R+ GTI G +LD L
Sbjct: 211 EAPEQRFTGTIVGSDNLDQL 270
Score = 55.1 bits (114), Expect(2) = 1e-024
Identities = 20/36 (55%), Positives = 25/36 (69%)
Frame = -1 / +1
Query: 353 WKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATP 246
W S WR+L+V WDES+ R RVS WEIEP ++P
Sbjct: 271 WPESSWRSLKVRWDESSTIPRPDRVSPWEIEPASSP 378
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 295,691,552
Number of Sequences: 312970
Number of extensions: 4672990
Number of successful extensions: 57823
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6659423313
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)