TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN21b10.yg.2.1
(574 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD663377.1|CD663377 UCRHV18_05bb12_b1 Drought-stressed D... 91 3e-034
gb|AV833885.1|AV833885 AV833885 K. Sato unpublished cDNA li... 134 3e-031
gb|BU973703.1|BU973703 HB25L04r BC Hordeum vulgare subsp. v... 111 2e-024
>gb|CD663377.1|CD663377 UCRHV18_05bb12_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_05bb12, mRNA sequence
Length = 844
Score = 90.9 bits (192), Expect(2) = 3e-034
Identities = 29/65 (44%), Positives = 47/65 (72%)
Frame = +3 / -2
Query: 321 RTGKWAVTDEEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVE 500
+ KW ++ +EWP + YPP+A+GPGY+IS DIAKF+V + LF +EDV+MG+W++
Sbjct: 621 KDSKWFISPKEWPVETYPPWAHGPGYIISRDIAKFVVRGHLERTLQLFKLEDVAMGIWIQ 442
Query: 501 KFNST 515
++ +
Sbjct: 441 QYKES 427
Score = 73.9 bits (155), Expect(2) = 3e-034
Identities = 30/62 (48%), Positives = 41/62 (66%)
Frame = +3 / -2
Query: 102 DIVILPFIDRYELVVLKTIAICEYGVQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKP 281
DI +PF+D Y L+ LKT+AIC +G + + A IMK DDD FVR+D V+ +K +N
Sbjct: 843 DIQFMPFVDYYTLITLKTVAICMFGTKIMPAKYIMKTDDDAFVRIDEVMSSLKKSNPRGL 664
Query: 282 LY 287
LY
Sbjct: 663 LY 658
>gb|AV833885.1|AV833885 AV833885 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags10a02, mRNA sequence
Length = 681
Score = 134 bits (288), Expect = 3e-031
Identities = 52/74 (70%), Positives = 60/74 (81%)
Frame = +3 / -3
Query: 294 NLNLLHRPLRTGKWAVTDEEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQXXXLFXME 473
++N HRPLR+GKWAVT EEWPE+ YP YANGPGYVIS DIA++IVS+ Q LF ME
Sbjct: 679 SMNYYHRPLRSGKWAVTYEEWPEEAYPSYANGPGYVISSDIARYIVSEFDTQTLRLFKME 500
Query: 474 DVSMGLWVEKFNST 515
DVSMG+WVEKFN T
Sbjct: 499 DVSMGMWVEKFNIT 458
>gb|BU973703.1|BU973703 HB25L04r BC Hordeum vulgare subsp. vulgare cDNA clone HB25L04
5-PRIME, mRNA sequence
Length = 464
Score = 111 bits (238), Expect = 2e-024
Identities = 48/48 (100%), Positives = 48/48 (100%)
Frame = +3 / +2
Query: 27 ARFFVALNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICE 170
ARFFVALNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICE
Sbjct: 320 ARFFVALNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICE 463
Score = 102 bits (218), Expect = 1e-021
Identities = 46/48 (95%), Positives = 48/48 (100%)
Frame = -1 / -3
Query: 169 SQIAIVLRTTSS*RSINGKMTMSPKYSASFFNITLTSFLEFSATKNRA 26
SQIAIVLRTTSS*RSINGKMT+SPKYSASFF+ITLTSFLEFSATKNRA
Sbjct: 462 SQIAIVLRTTSS*RSINGKMTISPKYSASFFSITLTSFLEFSATKNRA 319
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 257,741,889
Number of Sequences: 312970
Number of extensions: 3815664
Number of successful extensions: 39989
Number of sequences better than 1.0e-020: 3
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 5895922551
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)