TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCL35a05.yg.2.1
         (567 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CA015088.1|CA015088  HT13D15r HT Hordeum vulgare subsp. v...   140   6e-038

>gb|CA015088.1|CA015088 HT13D15r HT Hordeum vulgare subsp. vulgare cDNA clone HT13D15
           5-PRIME, mRNA sequence
          Length = 627

 Score =  140 bits (301), Expect(2) = 6e-038
 Identities = 57/126 (45%), Positives = 78/126 (61%)
 Frame = -3 / +3

                                                                       
Query: 448 EXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDPVIVFGEDTK 269
           E   VL  C+DG L +AK++  +H         V+L KFTTLLNTS +++PV  FG+D K
Sbjct: 3   EHEEVLLTCVDGFLGIAKISAFHHLEDVLDDLVVSLCKFTTLLNTSLVEEPVTAFGDDLK 182

                                                                       
Query: 268 ARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRLXXXXXXXXXXXXXSLPG 89
           AR+ATE +F IA  YG +IR+GWRN++DCIL  HK+GLLP R+             ++ G
Sbjct: 183 ARLATETLFIIANKYGHYIRTGWRNVLDCILRLHKLGLLPARVASDAADDSEVSAETVQG 362

                 
Query: 88  KHTSSA 71
           K   S+
Sbjct: 363 KPVPSS 380




 Score = 36.3 bits (73), Expect(2) = 6e-038
 Identities = 16/31 (51%), Positives = 20/31 (64%)
 Frame = -3 / +3

                                          
Query: 94  PGKHTSSAPQVLPISTPRKTYGLMGRFSQLL 2
           P   + S   +  + TPRK+ GLMGRFSQLL
Sbjct: 366 PVPSSISTSHIPVMGTPRKSSGLMGRFSQLL 458



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 183,901,001
Number of Sequences: 312970
Number of extensions: 2026817
Number of successful extensions: 19780
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 5811089133
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)