TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH30c02.yg.2.1
(722 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA015088.1|CA015088 HT13D15r HT Hordeum vulgare subsp. v... 198 4e-050
>gb|CA015088.1|CA015088 HT13D15r HT Hordeum vulgare subsp. vulgare cDNA clone HT13D15
5-PRIME, mRNA sequence
Length = 627
Score = 198 bits (426), Expect = 4e-050
Identities = 77/126 (61%), Positives = 102/126 (80%)
Frame = -2 / +3
Query: 448 EHEEVLTGCIDGFLSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTSYIDDPVTAFGEDTK 269
EHEEVL C+DGFL +AK++AF+HL DVL+DLVV+L KFTTLL TS +++PVTAFG+D K
Sbjct: 3 EHEEVLLTCVDGFLGIAKISAFHHLEDVLDDLVVSLCKFTTLLNTSLVEEPVTAFGDDLK 182
Query: 268 ARMATEAVFTIATVYGDHIRSGWRNIIDCILRLHKIGLLPGPLTGDTADDQDSSSKSLPG 89
AR+ATE +F IA YG +IR+GWRN++DCILRLHK+GLLP + D ADD + S++++ G
Sbjct: 183 ARLATETLFIIANKYGHYIRTGWRNVLDCILRLHKLGLLPARVASDAADDSEVSAETVQG 362
Query: 88 KLASSA 71
K S+
Sbjct: 363 KPVPSS 380
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 247,434,733
Number of Sequences: 312970
Number of extensions: 3184612
Number of successful extensions: 26121
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8016758001
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)