TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH17d04.yg.2.1
(637 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM099511.2|BM099511 EBes01_SQ003_H09_R embryo sac, 4-6 D... 349 7e-096
gb|BM443222.2|BM443222 EBro02_SQ003_D03_R root, 3 week, hyd... 266 9e-071
gb|CA016096.1|CA016096 HV10C18u HV Hordeum vulgare subsp. v... 239 1e-062
gb|CA016336.1|CA016336 HV09G16u HV Hordeum vulgare subsp. v... 226 1e-058
gb|CA005005.1|CA005005 HU12K09u HU Hordeum vulgare subsp. v... 190 1e-055
gb|CA008944.1|CA008944 HU12K09r HU Hordeum vulgare subsp. v... 184 4e-046
gb|BJ464838.1|BJ464838 BJ464838 K. Sato unpublished cDNA li... 169 1e-041
gb|AL500587.1|AL500587 AL500587 Hordeum vulgare Barke roots... 156 6e-040
>gb|BM099511.2|BM099511 EBes01_SQ003_H09_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ003_H09 5', mRNA sequence
Length = 689
Score = 349 bits (757), Expect = 7e-096
Identities = 143/180 (79%), Positives = 156/180 (86%)
Frame = -1 / +1
Query: 634 YRFSXXFEXXXEXDYXXEKFFQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVASV 455
Y+FS FE E DY EKFFQ V G+IPVVVGAPNIQEF PGE AILHI LDDV SV
Sbjct: 148 YKFSLAFENSNEEDYVTEKFFQSLVTGAIPVVVGAPNIQEFSPGEDAILHIKELDDVISV 327
Query: 454 AKTMKNIASNPDAFNQSLRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTP 275
AKTMK+IASNPDAFNQSLRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIH KEE+TP
Sbjct: 328 AKTMKHIASNPDAFNQSLRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHEKEEKTP 507
Query: 274 KFTNRPCSCSTKKGTIYHLFIRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPV 95
KF NR CSCS+K+GT+YHLF+RERGRFK+E+IY+RS QLTLGAL+SAV KF SL HVP+
Sbjct: 508 KFMNRSCSCSSKRGTVYHLFVRERGRFKTENIYLRSDQLTLGALKSAVHDKFSSLKHVPI 687
Score = 284 bits (615), Expect = 3e-076
Identities = 133/181 (73%), Positives = 140/181 (77%)
Frame = +3 / -1
Query: 93 HTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDLNRPLSRINKW*IVPFLVEQLQGRFVN 272
H GT LR+LNL TAD KAP+VN DL *M S LNRPLS NKW VP L+EQL RF+N
Sbjct: 689 HIGTCLRELNLSCTADFKAPKVN*SDLR*MFSVLNRPLSLTNKWYTVPLLLEQLHERFIN 510
Query: 273 FGVLSSFKWILVAICIQRRQDE*TAAMSIRALKESDGPSYFHLKD*LKASGFEAIFFIVL 452
FGV SSF I VAICIQRRQDE*TAAMSI ALKESDGPSYFHLKD*LKASGF+AI FIV
Sbjct: 509 FGVFSSFS*IFVAICIQRRQDE*TAAMSISALKESDGPSYFHLKD*LKASGFDAICFIVF 330
Query: 453 ATEATSSSXLICNIAPSPGXNS*IFGAPTTTGIEPXTXX*KNFSXX*SXSXXXSNXXLNL 632
ATE TSSS L+CNIA SPG NS*IFGAPTTTG+ P T *KNFS *S S S LNL
Sbjct: 329 ATEMTSSSSLMCNIASSPGENS*IFGAPTTTGMAPVTSD*KNFSVT*SSSLEFSKAKLNL 150
Query: 633 * 635
*
Sbjct: 149 * 147
Score = 212 bits (458), Expect = 1e-054
Identities = 110/181 (60%), Positives = 124/181 (68%)
Frame = -3 / +3
Query: 635 LQIQXXI*XXX*XGLXX*KVFSVXSXRFYSGCCWCSKYSRVXSGRRRNITY*XA**CCFS 456
LQIQ * * L KV SV R +S C W ++YSRV S RR NIT+ A**C FS
Sbjct: 147 LQIQLGF*EF**GRLCYRKVLSVTGNRGHSSCRWRTEYSRVFSRRRCNITHQGA**CHFS 326
Query: 455 C*DNEKYCFKP*CLQSIFEVEV*WSIRFFQSSY*HGSGSFILSSLYTYCYQDPFKGGKDS 276
C DNE YC K *C SIFEVEV*WSI FFQ +Y*HGSGSFILS LYTY Y+D K K+S
Sbjct: 327 CEDNETYCIKS*CF*SIFEVEV*WSI*FFQGTY*HGSGSFILSPLYTYRYEDS*KRRKNS 506
Query: 275 KIYKSSL*LFHQKGNNLPLIYPRERAV*VREHLHEIRPVNSGSLGIRSAR*I*VPQPRSC 96
KIY+S +*L QK N++PLI RERAV* REHL +IR VN S + AR*I*+PQ S
Sbjct: 507 KIYESLV*LLQQKRNSIPLICQRERAV*DREHLSKIRSVNFRSFEVCCAR*I*LPQACSY 686
Query: 95 M 93
M
Sbjct: 687 M 689
Score = 210 bits (453), Expect = 6e-054
Identities = 96/144 (66%), Positives = 108/144 (75%)
Frame = +2 / -3
Query: 95 YRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLSDK*VVNCSLFGGTATRTICKF 274
YRN++EG KF H Q S S*L *S +N L LKPPSLSDK*VV CS F G TR I KF
Sbjct: 687 YRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPPSLSDK*VVYCSSFAGATTRAIHKF 508
Query: 275 WSPFLL*MDLGSNMYTKTTR*MNRCHVNKSFERIGWTIILPPQRLIEGIRV*SNIFHCLS 454
WS F M+L S+MYTK T *MNRCHVNK ERI WTIILPPQRLI+ IR+* N+FHCL
Sbjct: 507 WSFFFFFMNLRSDMYTKATG*MNRCHVNKCLERIRWTIILPPQRLIKSIRI*CNMFHCLR 328
Query: 455 N*SNIIKLXNM*YCAFSRXKLLNI 526
N*++IIKL ++*YC FS KLLNI
Sbjct: 327 N*NDIIKLLDV*YCIFSWRKLLNI 256
Score = 207 bits (447), Expect = 4e-053
Identities = 110/179 (61%), Positives = 124/179 (69%)
Frame = +1 / -2
Query: 100 ERG*GT*IYRALRIPRLPELTGLISCKCSLT*TALSLG*ISGKLFPFWWNSYKDDL*ILE 279
E *G+*IYRA + +L +LT LI KCSL+*TALSL ISG LF F W++Y D *ILE
Sbjct: 682 EHA*GS*IYRAQQTSKLLKLTDLILDKCSLS*TALSL*QISGILFLFCWSNYTSDS*ILE 503
Query: 280 SFPPLNGSW*QYVYKDDKMNEPLPCQ*EL*KNRMDHHTSTSKID*RHQGLKQYFSLS*QL 459
F + S *+YVYK D+MNEPLPCQ* *KN+MDHHTSTSKID*+HQ L QY SLS QL
Sbjct: 502 FFLLFHESS*RYVYKGDRMNEPLPCQ*VP*KNQMDHHTSTSKID*KHQDLMQYVSLSSQL 323
Query: 460 KQHHQAX*YVILRLLPEXTLEYLEHQQQPE*NLXLXTEKTFXXHNPXXXXSQXXX*ICS 636
K HHQA * VIL LL E TLEY Q+Q E L TE+TF HN SQ *ICS
Sbjct: 322 K*HHQAP*CVILHLLLEKTLEYSVRQRQLEWPLLPVTERTFL*HNLPH*NSQKPS*ICS 146
Score = 148 bits (318), Expect(2) = 2e-037
Identities = 70/106 (66%), Positives = 79/106 (74%)
Frame = -2 / +2
Query: 411 INL*GGSMMVHPILSKLLLTWQRFIHLVVFVYILLPRSI*RRKGLQNLQIVLVAVPPKRE 232
INL*GGSMMVH ILS+ LLTWQRFIH V FVYI L R + ++K LQNL I V P K E
Sbjct: 371 INL*GGSMMVHLILSRHLLTWQRFIHPVAFVYISLRRFMKKKKKLQNL*IARVVAPAKEE 550
Query: 231 QFTTYLSEREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLY 94
Q+TTYLSEREGGL QR F *DQ S*L E + C++NL PS+ FLY
Sbjct: 551 QYTTYLSEREGGLRQRTFI*DQIS*L*EL*SLLCTINLAPSSMFLY 688
Score = 26.7 bits (52), Expect(2) = 2e-037
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = -2 / +2
Query: 528 QIFKSXLREKAQYY 487
+IFKS L+EK QYY
Sbjct: 254 RIFKSFLQEKMQYY 295
>gb|BM443222.2|BM443222 EBro02_SQ003_D03_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ003_D03 5', mRNA sequence
Length = 383
Score = 266 bits (575), Expect = 9e-071
Identities = 103/126 (81%), Positives = 117/126 (92%)
Frame = -1 / +3
Query: 403 LRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSTKKGTIY 224
LRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIH KEE+TPKF NR CSCS+K+GT+Y
Sbjct: 3 LRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHEKEEKTPKFMNRSCSCSSKRGTVY 182
Query: 223 HLFIRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKL 44
HLF+RERGRFK+E+IY+RS QLTLGAL+SAV KF SL HVP+WKDERP SIRGGD+LK+
Sbjct: 183 HLFVRERGRFKTENIYLRSDQLTLGALKSAVHDKFSSLKHVPIWKDERPSSIRGGDELKV 362
Query: 43 YRIYPV 26
Y+IYP+
Sbjct: 363 YKIYPI 380
Score = 188 bits (405), Expect = 2e-047
Identities = 84/125 (67%), Positives = 90/125 (72%)
Frame = +2 / -1
Query: 29 WVNSV*FQVIPTSNARWPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSL 208
W+N V Q IPTSN WPFILPYRN++EG KF H Q S S*L *S +N L LKPPSL
Sbjct: 377 WINFVHLQFIPTSNT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPPSL 198
Query: 209 SDK*VVNCSLFGGTATRTICKFWSPFLL*MDLGSNMYTKTTR*MNRCHVNKSFERIGWTI 388
SDK*VV CS F G TR I KFWS F M+L S+MYTK T *MNRCHVNK ERI WTI
Sbjct: 197 SDK*VVYCSSFAGATTRAIHKFWSFFFFFMNLRSDMYTKATG*MNRCHVNKCLERIRWTI 18
Query: 389 ILPPQ 403
ILPPQ
Sbjct: 17 ILPPQ 3
Score = 183 bits (394), Expect = 8e-046
Identities = 85/125 (68%), Positives = 89/125 (71%)
Frame = +3 / -2
Query: 30 G*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDLNRPLS 209
G*ILY F FH GT LR+LNL TAD KAP+VN DL *M S LNRPLS
Sbjct: 376 G*ILYTFNSSPPRILDGLSSFHIGTCLRELNLSCTADFKAPKVN*SDLR*MFSVLNRPLS 197
Query: 210 RINKW*IVPFLVEQLQGRFVNFGVLSSFKWILVAICIQRRQDE*TAAMSIRALKESDGPS 389
NKW VP L+EQL RF+NFGV SSF I VAICIQRRQDE*TAAMSI ALKESDGPS
Sbjct: 196 LTNKWYTVPLLLEQLHERFINFGVFSSFS*IFVAICIQRRQDE*TAAMSISALKESDGPS 17
Query: 390 YFHLK 404
YFHLK
Sbjct: 16 YFHLK 2
Score = 176 bits (379), Expect = 1e-043
Identities = 82/126 (65%), Positives = 93/126 (73%)
Frame = -2 / +1
Query: 405 L*GGSMMVHPILSKLLLTWQRFIHLVVFVYILLPRSI*RRKGLQNLQIVLVAVPPKREQF 226
L*GGSMMVH ILS+ LLTWQRFIH V FVYI L R + ++K LQNL I V P K EQ+
Sbjct: 1 L*GGSMMVHLILSRHLLTWQRFIHPVAFVYISLRRFMKKKKKLQNL*IARVVAPAKEEQY 180
Query: 225 TTYLSEREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGHRALEVGMT*N 46
TTYLSEREGGL QR F *DQ S*L E + C++NL PS+ FLYGRMKGH+ EVGM *
Sbjct: 181 TTYLSEREGGLRQRTFI*DQIS*L*EL*SLLCTINLAPSSMFLYGRMKGHQVFEVGMN*R 360
Query: 45 YTEFTQ 28
T+F Q
Sbjct: 361 CTKFIQ 378
Score = 164 bits (353), Expect = 4e-040
Identities = 81/124 (65%), Positives = 94/124 (75%)
Frame = -3 / +2
Query: 404 FEVEV*WSIRFFQSSY*HGSGSFILSSLYTYCYQDPFKGGKDSKIYKSSL*LFHQKGNNL 225
FEVEV*WSI FFQ +Y*HGSGSFILS LYTY Y+D K K+SKIY+S +*L QK N++
Sbjct: 2 FEVEV*WSI*FFQGTY*HGSGSFILSPLYTYRYEDS*KRRKNSKIYESLV*LLQQKRNSI 181
Query: 224 PLIYPRERAV*VREHLHEIRPVNSGSLGIRSAR*I*VPQPRSCMEG*KATEH*RWG*PEI 45
PLI RERAV* REHL +IR VN S + AR*I*+PQ S MEG*KA ++ RWG* E
Sbjct: 182 PLICQRERAV*DREHLSKIRSVNFRSFEVCCAR*I*LPQACSYMEG*KAIKYSRWG*IEG 361
Query: 44 IQNL 33
+QNL
Sbjct: 362 VQNL 373
Score = 161 bits (347), Expect = 2e-039
Identities = 80/126 (63%), Positives = 92/126 (73%)
Frame = +1 / -3
Query: 28 LGKFCIISGHPHL*CSVAFHPSIQERG*GT*IYRALRIPRLPELTGLISCKCSLT*TALS 207
L KFC S HPHL +AFHPSI E *G+*IYRA + +L +LT LI KCSL+*TALS
Sbjct: 378 LDKFCTPSIHPHLEYLMAFHPSI*EHA*GS*IYRAQQTSKLLKLTDLILDKCSLS*TALS 199
Query: 208 LG*ISGKLFPFWWNSYKDDL*ILESFPPLNGSW*QYVYKDDKMNEPLPCQ*EL*KNRMDH 387
L ISG LF F W++Y D *ILE F + S *+YVYK D+MNEPLPCQ* *KN+MDH
Sbjct: 198 L*QISGILFLFCWSNYTSDS*ILEFFLLFHESS*RYVYKGDRMNEPLPCQ*VP*KNQMDH 19
Query: 388 HTSTSK 405
HTSTSK
Sbjct: 18 HTSTSK 1
>gb|CA016096.1|CA016096 HV10C18u HV Hordeum vulgare subsp. vulgare cDNA clone HV10C18
3-PRIME, mRNA sequence
Length = 556
Score = 239 bits (516), Expect = 1e-062
Identities = 93/117 (79%), Positives = 108/117 (92%)
Frame = -1 / -1
Query: 352 MAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSTKKGTIYHLFIRERGRFKSESIYM 173
MAAVHSSCRLCIHIATKIH KEE+TPKF NR CSCS+K+GT+YHLF+RERGRFK+E+IY+
Sbjct: 556 MAAVHSSCRLCIHIATKIHEKEEKTPKFMNRSCSCSSKRGTVYHLFVRERGRFKTENIYL 377
Query: 172 RSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
RS QLTLGAL+SAV KF SL HVP+WKDERP SIRGGD+LK+Y+IYP+GLT+RQAL
Sbjct: 376 RSDQLTLGALKSAVHDKFSSLKHVPIWKDERPSSIRGGDELKVYKIYPIGLTERQAL 206
Score = 155 bits (334), Expect = 2e-037
Identities = 71/110 (64%), Positives = 77/110 (70%)
Frame = +2 / +2
Query: 23 TDWVNSV*FQVIPTSNARWPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPP 202
T W+N V Q IPTSN WPFILPYRN++EG KF H Q S S*L *S +N L LKPP
Sbjct: 227 TYWINFVHLQFIPTSNT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPP 406
Query: 203 SLSDK*VVNCSLFGGTATRTICKFWSPFLL*MDLGSNMYTKTTR*MNRCH 352
SLSDK*VV CS F G TR I KFWS F M+L S+MYTK T *MNRCH
Sbjct: 407 SLSDK*VVYCSSFAGATTRAIHKFWSFFFFFMNLRSDMYTKATG*MNRCH 556
Score = 152 bits (326), Expect = 2e-036
Identities = 72/113 (63%), Positives = 76/113 (67%)
Frame = +3 / +3
Query: 12 RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSD 191
R VRP G*ILY F FH GT LR+LNL TAD KAP+VN DL *M S
Sbjct: 216 RSVRPIG*ILYTFNSSPPRILDGLSSFHIGTCLRELNLSCTADFKAPKVN*SDLR*MFSV 395
Query: 192 LNRPLSRINKW*IVPFLVEQLQGRFVNFGVLSSFKWILVAICIQRRQDE*TAA 350
LNRPLS NKW VP L+EQL RF+NFGV SSF I VAICIQRRQDE*TAA
Sbjct: 396 LNRPLSLTNKWYTVPLLLEQLHERFINFGVFSSFS*IFVAICIQRRQDE*TAA 554
Score = 148 bits (318), Expect = 2e-035
Identities = 69/113 (61%), Positives = 80/113 (70%)
Frame = -2 / -2
Query: 351 WQRFIHLVVFVYILLPRSI*RRKGLQNLQIVLVAVPPKREQFTTYLSEREGGLSQRAFT* 172
WQRFIH V FVYI L R + ++K LQNL I V P K EQ+TTYLSEREGGL QR F *
Sbjct: 555 WQRFIHPVAFVYISLRRFMKKKKKLQNL*IARVVAPAKEEQYTTYLSEREGGLRQRTFI* 376
Query: 171 DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGHRALEVGMT*NYTEFTQSV*RN 13
DQ S*L E + C++NL PS+ FLYGRMKGH+ EVGM * T+F Q V +N
Sbjct: 375 DQIS*L*EL*SLLCTINLAPSSMFLYGRMKGHQVFEVGMN*RCTKFIQ*VLQN 217
Score = 136 bits (291), Expect = 1e-031
Identities = 69/114 (60%), Positives = 83/114 (72%)
Frame = -3 / -3
Query: 350 GSGSFILSSLYTYCYQDPFKGGKDSKIYKSSL*LFHQKGNNLPLIYPRERAV*VREHLHE 171
GSGSFILS LYTY Y+D K K+SKIY+S +*L QK N++PLI RERAV* REHL +
Sbjct: 554 GSGSFILSPLYTYRYEDS*KRRKNSKIYESLV*LLQQKRNSIPLICQRERAV*DREHLSK 375
Query: 170 IRPVNSGSLGIRSAR*I*VPQPRSCMEG*KATEH*RWG*PEIIQNLPSRSNATS 9
IR VN S + AR*I*+PQ S MEG*KA ++ RWG* E +QNL +RS T+
Sbjct: 374 IRSVNFRSFEVCCAR*I*LPQACSYMEG*KAIKYSRWG*IEGVQNLSNRSYRTT 213
Score = 130 bits (278), Expect = 8e-030
Identities = 66/110 (60%), Positives = 77/110 (70%)
Frame = +1 / +1
Query: 22 DRLGKFCIISGHPHL*CSVAFHPSIQERG*GT*IYRALRIPRLPELTGLISCKCSLT*TA 201
D L KFC S HPHL +AFHPSI E *G+*IYRA + +L +LT LI KCSL+*TA
Sbjct: 226 DLLDKFCTPSIHPHLEYLMAFHPSI*EHA*GS*IYRAQQTSKLLKLTDLILDKCSLS*TA 405
Query: 202 LSLG*ISGKLFPFWWNSYKDDL*ILESFPPLNGSW*QYVYKDDKMNEPLP 351
LSL ISG LF F W++Y D *ILE F + S *+YVYK D+MNEPLP
Sbjct: 406 LSL*QISGILFLFCWSNYTSDS*ILEFFLLFHESS*RYVYKGDRMNEPLP 555
>gb|CA016336.1|CA016336 HV09G16u HV Hordeum vulgare subsp. vulgare cDNA clone HV09G16
3-PRIME, mRNA sequence
Length = 540
Score = 226 bits (487), Expect = 1e-058
Identities = 87/111 (78%), Positives = 102/111 (91%)
Frame = -1 / -3
Query: 334 SCRLCIHIATKIHLKEERTPKFTNRPCSCSTKKGTIYHLFIRERGRFKSESIYMRSGQLT 155
SCRLCIHIATKIH KEE+TPKF NR CSCS+K+GT+YHLF+RERGRFK+E+IY+RS QLT
Sbjct: 538 SCRLCIHIATKIHEKEEKTPKFMNRSCSCSSKRGTVYHLFVRERGRFKTENIYLRSDQLT 359
Query: 154 LGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
LGAL+SAV KF SL HVP+WKDERP SIRGGD+LK+Y+IYP+GLT+RQAL
Sbjct: 358 LGALKSAVHDKFSSLKHVPIWKDERPSSIRGGDELKVYKIYPIGLTERQAL 206
Score = 143 bits (307), Expect = 8e-034
Identities = 67/108 (62%), Positives = 71/108 (65%)
Frame = +3 / +3
Query: 12 RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSD 191
R VRP G*ILY F FH GT LR+LNL TAD KAP+VN DL *M S
Sbjct: 216 RSVRPIG*ILYTFNSSPPRILDGLSSFHIGTCLRELNLSCTADFKAPKVN*SDLR*MFSV 395
Query: 192 LNRPLSRINKW*IVPFLVEQLQGRFVNFGVLSSFKWILVAICIQRRQD 335
LNRPLS NKW VP L+EQL RF+NFGV SSF I VAICIQRRQD
Sbjct: 396 LNRPLSLTNKWYTVPLLLEQLHERFINFGVFSSFS*IFVAICIQRRQD 539
Score = 141 bits (302), Expect = 4e-033
Identities = 65/103 (63%), Positives = 71/103 (68%)
Frame = +2 / +2
Query: 23 TDWVNSV*FQVIPTSNARWPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPP 202
T W+N V Q IPTSN WPFILPYRN++EG KF H Q S S*L *S +N L LKPP
Sbjct: 227 TYWINFVHLQFIPTSNT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPP 406
Query: 203 SLSDK*VVNCSLFGGTATRTICKFWSPFLL*MDLGSNMYTKTT 331
SLSDK*VV CS F G TR I KFWS F M+L S+MYTK T
Sbjct: 407 SLSDK*VVYCSSFAGATTRAIHKFWSFFFFFMNLRSDMYTKAT 535
Score = 134 bits (287), Expect = 5e-031
Identities = 64/108 (59%), Positives = 75/108 (69%)
Frame = -2 / -1
Query: 336 HLVVFVYILLPRSI*RRKGLQNLQIVLVAVPPKREQFTTYLSEREGGLSQRAFT*DQAS* 157
H V FVYI L R + ++K LQNL I V P K EQ+TTYLSEREGGL QR F *DQ S*
Sbjct: 540 HPVAFVYISLRRFMKKKKKLQNL*IARVVAPAKEEQYTTYLSEREGGLRQRTFI*DQIS* 361
Query: 156 LWEPWNPQCSVNLGPSTTFLYGRMKGHRALEVGMT*NYTEFTQSV*RN 13
L E + C++NL PS+ FLYGRMKGH+ EVGM * T+F Q V +N
Sbjct: 360 L*EL*SLLCTINLAPSSMFLYGRMKGHQVFEVGMN*RCTKFIQ*VLQN 217
Score = 124 bits (265), Expect = 5e-028
Identities = 64/109 (58%), Positives = 78/109 (71%)
Frame = -3 / -2
Query: 335 ILSSLYTYCYQDPFKGGKDSKIYKSSL*LFHQKGNNLPLIYPRERAV*VREHLHEIRPVN 156
ILS LYTY Y+D K K+SKIY+S +*L QK N++PLI RERAV* REHL +IR VN
Sbjct: 539 ILSPLYTYRYEDS*KRRKNSKIYESLV*LLQQKRNSIPLICQRERAV*DREHLSKIRSVN 360
Query: 155 SGSLGIRSAR*I*VPQPRSCMEG*KATEH*RWG*PEIIQNLPSRSNATS 9
S + AR*I*+PQ S MEG*KA ++ RWG* E +QNL +RS T+
Sbjct: 359 FRSFEVCCAR*I*LPQACSYMEG*KAIKYSRWG*IEGVQNLSNRSYRTT 213
Score = 116 bits (249), Expect = 8e-026
Identities = 61/105 (58%), Positives = 72/105 (68%)
Frame = +1 / +1
Query: 22 DRLGKFCIISGHPHL*CSVAFHPSIQERG*GT*IYRALRIPRLPELTGLISCKCSLT*TA 201
D L KFC S HPHL +AFHPSI E *G+*IYRA + +L +LT LI KCSL+*TA
Sbjct: 226 DLLDKFCTPSIHPHLEYLMAFHPSI*EHA*GS*IYRAQQTSKLLKLTDLILDKCSLS*TA 405
Query: 202 LSLG*ISGKLFPFWWNSYKDDL*ILESFPPLNGSW*QYVYKDDKM 336
LSL ISG LF F W++Y D *ILE F + S *+YVYK D+M
Sbjct: 406 LSL*QISGILFLFCWSNYTSDS*ILEFFLLFHESS*RYVYKGDRM 540
>gb|CA005005.1|CA005005 HU12K09u HU Hordeum vulgare subsp. vulgare cDNA clone HU12K09
3-PRIME, mRNA sequence
Length = 543
Score = 190 bits (409), Expect(2) = 1e-055
Identities = 72/97 (74%), Positives = 89/97 (91%)
Frame = -1 / -1
Query: 292 KEERTPKFTNRPCSCSTKKGTIYHLFIRERGRFKSESIYMRSGQLTLGALESAVLGKFRS 113
K+++TPKF NR CSCS+K+GT+YHLF+RERGRFK+E+IY+RS QLTLGAL+SAV KF S
Sbjct: 495 KKKKTPKFMNRSCSCSSKRGTVYHLFVRERGRFKTENIYLRSDQLTLGALKSAVHDKFSS 316
Query: 112 LNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
L HVP+WKDERP SIRGGD+LK+Y+IYP+GLT+RQAL
Sbjct: 315 LKHVPIWKDERPSSIRGGDELKVYKIYPIGLTERQAL 205
Score = 128 bits (274), Expect(2) = 8e-034
Identities = 59/90 (65%), Positives = 63/90 (70%)
Frame = +2 / +1
Query: 23 TDWVNSV*FQVIPTSNARWPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPP 202
T W+N V Q IPTSN WPFILPYRN++EG KF H Q S S*L *S +N L LKPP
Sbjct: 226 TYWINFVHLQFIPTSNT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPP 405
Query: 203 SLSDK*VVNCSLFGGTATRTICKFWSPFLL 292
SLSDK*VV CS F G TR I KFWS FLL
Sbjct: 406 SLSDK*VVYCSSFAGATTRAIHKFWSFFLL 495
Score = 123 bits (263), Expect(2) = 5e-030
Identities = 58/93 (62%), Positives = 66/93 (70%)
Frame = -2 / -2
Query: 291 RRKGLQNLQIVLVAVPPKREQFTTYLSEREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGP 112
RRK LQNL I V P K EQ+TTYLSEREGGL QR F *DQ S*L E + C++NL P
Sbjct: 494 RRKKLQNL*IARVVAPAKEEQYTTYLSEREGGLRQRTFI*DQIS*L*EL*SLLCTINLAP 315
Query: 111 STTFLYGRMKGHRALEVGMT*NYTEFTQSV*RN 13
S+ FLYGRMKGH+ EVGM * T+F Q V +N
Sbjct: 314 SSMFLYGRMKGHQVFEVGMN*RCTKFIQ*VLQN 216
Score = 114 bits (244), Expect(2) = 4e-026
Identities = 54/94 (57%), Positives = 58/94 (61%)
Frame = +3 / +2
Query: 12 RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSD 191
R VRP G*ILY F FH GT LR+LNL TAD KAP+VN DL *M S
Sbjct: 215 RSVRPIG*ILYTFNSSPPRILDGLSSFHIGTCLRELNLSCTADFKAPKVN*SDLR*MFSV 394
Query: 192 LNRPLSRINKW*IVPFLVEQLQGRFVNFGVLSSF 293
LNRPLS NKW VP L+EQL RF+NFGV F
Sbjct: 395 LNRPLSLTNKWYTVPLLLEQLHERFINFGVFFFF 496
Score = 105 bits (224), Expect = 2e-022
Identities = 55/95 (57%), Positives = 68/95 (71%)
Frame = -3 / -3
Query: 293 KGGKDSKIYKSSL*LFHQKGNNLPLIYPRERAV*VREHLHEIRPVNSGSLGIRSAR*I*V 114
K K+SKIY+S +*L QK N++PLI RERAV* REHL +IR VN S + AR*I*+
Sbjct: 496 KEEKNSKIYESLV*LLQQKRNSIPLICQRERAV*DREHLSKIRSVNFRSFEVCCAR*I*L 317
Query: 113 PQPRSCMEG*KATEH*RWG*PEIIQNLPSRSNATS 9
PQ S MEG*KA ++ RWG* E +QNL +RS T+
Sbjct: 316 PQACSYMEG*KAIKYSRWG*IEGVQNLSNRSYRTT 212
Score = 102 bits (218), Expect = 2e-021
Identities = 53/88 (60%), Positives = 61/88 (69%)
Frame = +1 / +3
Query: 22 DRLGKFCIISGHPHL*CSVAFHPSIQERG*GT*IYRALRIPRLPELTGLISCKCSLT*TA 201
D L KFC S HPHL +AFHPSI E *G+*IYRA + +L +LT LI KCSL+*TA
Sbjct: 225 DLLDKFCTPSIHPHLEYLMAFHPSI*EHA*GS*IYRAQQTSKLLKLTDLILDKCSLS*TA 404
Query: 202 LSLG*ISGKLFPFWWNSYKDDL*ILESF 285
LSL ISG LF F W++Y D *ILE F
Sbjct: 405 LSL*QISGILFLFCWSNYTSDS*ILEFF 488
Score = 46.4 bits (95), Expect(2) = 1e-055
Identities = 18/22 (81%), Positives = 19/22 (86%)
Frame = -1 / -3
Query: 337 SSCRLCIHIATKIHLKEERTPK 272
SSCRLCIHIATKIH KEE+ K
Sbjct: 541 SSCRLCIHIATKIHEKEEKNSK 476
Score = 35.0 bits (70), Expect(2) = 8e-034
Identities = 15/18 (83%), Positives = 15/18 (83%)
Frame = +3 / +3
Query: 285 SSFKWILVAICIQRRQDE 338
SSF I VAICIQRRQDE
Sbjct: 489 SSFS*IFVAICIQRRQDE 542
Score = 27.2 bits (53), Expect(2) = 5e-030
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = -3 / -2
Query: 338 FILSSLYTYCYQDPFKGGK 282
FILS LYTY Y+D K K
Sbjct: 542 FILSPLYTYRYEDS*KRRK 486
Score = 23.1 bits (44), Expect(2) = 4e-026
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +1 / +1
Query: 301 SW*QYVYKDDKMN 339
S *+YVYK D+MN
Sbjct: 505 SS*RYVYKGDRMN 543
>gb|CA008944.1|CA008944 HU12K09r HU Hordeum vulgare subsp. vulgare cDNA clone HU12K09
5-PRIME, mRNA sequence
Length = 551
Score = 184 bits (396), Expect = 4e-046
Identities = 77/96 (80%), Positives = 80/96 (83%)
Frame = -1 / +2
Query: 634 YRFSXXFEXXXEXDYXXEKFFQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVASV 455
Y+FS FE E DY EKFFQ V G+IPVVVGAPNIQEF PGE AILHI LDDV SV
Sbjct: 263 YKFSLAFENSNEEDYVTEKFFQSLVTGAIPVVVGAPNIQEFSPGEDAILHIKELDDVISV 442
Query: 454 AKTMKNIASNPDAFNQSLRWKYDGPSDSFKALIDMA 347
AKTMK+IASNPDAFNQSLRWKYDGPSDSFKALIDMA
Sbjct: 443 AKTMKHIASNPDAFNQSLRWKYDGPSDSFKALIDMA 550
Score = 156 bits (336), Expect = 8e-038
Identities = 73/96 (76%), Positives = 76/96 (79%)
Frame = +3 / -3
Query: 348 AMSIRALKESDGPSYFHLKD*LKASGFEAIFFIVLATEATSSSXLICNIAPSPGXNS*IF 527
AMSI ALKESDGPSYFHLKD*LKASGF+AI FIV ATE TSSS L+CNIA SPG NS*IF
Sbjct: 549 AMSISALKESDGPSYFHLKD*LKASGFDAICFIVFATEMTSSSSLMCNIASSPGENS*IF 370
Query: 528 GAPTTTGIEPXTXX*KNFSXX*SXSXXXSNXXLNL* 635
GAPTTTG+ P T *KNFS *S S S LNL*
Sbjct: 369 GAPTTTGMAPVTSD*KNFSVT*SSSLEFSKAKLNL* 262
Score = 118 bits (252), Expect = 3e-026
Identities = 62/97 (63%), Positives = 66/97 (68%)
Frame = +1 / -1
Query: 346 LPCQ*EL*KNRMDHHTSTSKID*RHQGLKQYFSLS*QLKQHHQAX*YVILRLLPEXTLEY 525
LPCQ* *KN+MDHHTSTSKID*+HQ L QY SLS QLK HHQA * VIL LL E TLEY
Sbjct: 551 LPCQ*VP*KNQMDHHTSTSKID*KHQDLMQYVSLSSQLK*HHQAP*CVILHLLLEKTLEY 372
Query: 526 LEHQQQPE*NLXLXTEKTFXXHNPXXXXSQXXX*ICS 636
Q+Q E L TE+TF HN SQ *ICS
Sbjct: 371 SVRQRQLEWPLLPVTERTFL*HNLPH*NSQKPS*ICS 261
Score = 114 bits (243), Expect = 6e-025
Identities = 58/96 (60%), Positives = 63/96 (65%)
Frame = -3 / +1
Query: 635 LQIQXXI*XXX*XGLXX*KVFSVXSXRFYSGCCWCSKYSRVXSGRRRNITY*XA**CCFS 456
LQIQ * * L KV SV R +S C W ++YSRV S RR NIT+ A**C FS
Sbjct: 262 LQIQLGF*EF**GRLCYRKVLSVTGNRGHSSCRWRTEYSRVFSRRRCNITHQGA**CHFS 441
Query: 455 C*DNEKYCFKP*CLQSIFEVEV*WSIRFFQSSY*HG 348
C DNE YC K *C SIFEVEV*WSI FFQ +Y*HG
Sbjct: 442 CEDNETYCIKS*CF*SIFEVEV*WSI*FFQGTY*HG 549
Score = 104 bits (221), Expect = 6e-022
Identities = 43/60 (71%), Positives = 50/60 (83%)
Frame = +2 / -2
Query: 347 CHVNKSFERIGWTIILPPQRLIEGIRV*SNIFHCLSN*SNIIKLXNM*YCAFSRXKLLNI 526
CHVNK ERI WTIILPPQRLI+ IR+* N+FHCL N*++IIKL ++*YC FS KLLNI
Sbjct: 550 CHVNKCLERIRWTIILPPQRLIKSIRI*CNMFHCLRN*NDIIKLLDV*YCIFSWRKLLNI 371
>gb|BJ464838.1|BJ464838 BJ464838 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags36d24 5', mRNA sequence
Length = 571
Score = 169 bits (364), Expect = 1e-041
Identities = 70/89 (78%), Positives = 73/89 (82%)
Frame = -1 / +1
Query: 634 YRFSXXFEXXXEXDYXXEKFFQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVASV 455
Y+FS FE E DY EKFFQ V G+IPVVVGAPNIQEF PGE AILHI LDDV SV
Sbjct: 304 YKFSLAFENSNEEDYVTEKFFQSLVTGAIPVVVGAPNIQEFSPGEDAILHIKELDDVISV 483
Query: 454 AKTMKNIASNPDAFNQSLRWKYDGPSDSF 368
AKTMK+IASNPDAFNQSLRWKYDGPSDSF
Sbjct: 484 AKTMKHIASNPDAFNQSLRWKYDGPSDSF 570
Score = 145 bits (312), Expect = 2e-034
Identities = 67/89 (75%), Positives = 70/89 (78%)
Frame = +3 / -3
Query: 369 KESDGPSYFHLKD*LKASGFEAIFFIVLATEATSSSXLICNIAPSPGXNS*IFGAPTTTG 548
KESDGPSYFHLKD*LKASGF+AI FIV ATE TSSS L+CNIA SPG NS*IFGAPTTTG
Sbjct: 569 KESDGPSYFHLKD*LKASGFDAICFIVFATEMTSSSSLMCNIASSPGENS*IFGAPTTTG 390
Query: 549 IEPXTXX*KNFSXX*SXSXXXSNXXLNL* 635
+ P T *KNFS *S S S LNL*
Sbjct: 389 MAPVTSD*KNFSVT*SSSLEFSKAKLNL* 303
Score = 108 bits (231), Expect = 2e-023
Identities = 57/90 (63%), Positives = 61/90 (67%)
Frame = +1 / -1
Query: 367 *KNRMDHHTSTSKID*RHQGLKQYFSLS*QLKQHHQAX*YVILRLLPEXTLEYLEHQQQP 546
*KN+MDHHTSTSKID*+HQ L QY SLS QLK HHQA * VIL LL E TLEY Q+Q
Sbjct: 571 *KNQMDHHTSTSKID*KHQDLMQYVSLSSQLK*HHQAP*CVILHLLLEKTLEYSVRQRQL 392
Query: 547 E*NLXLXTEKTFXXHNPXXXXSQXXX*ICS 636
E L TE+TF HN SQ *ICS
Sbjct: 391 EWPLLPVTERTFL*HNLPH*NSQKPS*ICS 302
Score = 101 bits (215), Expect = 4e-021
Identities = 53/89 (59%), Positives = 57/89 (64%)
Frame = -3 / +3
Query: 635 LQIQXXI*XXX*XGLXX*KVFSVXSXRFYSGCCWCSKYSRVXSGRRRNITY*XA**CCFS 456
LQIQ * * L KV SV R +S C W ++YSRV S RR NIT+ A**C FS
Sbjct: 303 LQIQLGF*EF**GRLCYRKVLSVTGNRGHSSCRWRTEYSRVFSRRRCNITHQGA**CHFS 482
Query: 455 C*DNEKYCFKP*CLQSIFEVEV*WSIRFF 369
C DNE YC K *C SIFEVEV*WSI FF
Sbjct: 483 CEDNETYCIKS*CF*SIFEVEV*WSI*FF 569
>gb|AL500587.1|AL500587 AL500587 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW01C14u 3', mRNA sequence
Length = 526
Score = 156 bits (335), Expect(2) = 6e-040
Identities = 60/81 (74%), Positives = 74/81 (91%)
Frame = -1 / -2
Query: 244 TKKGTIYHLFIRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIR 65
+K+GT+YHL +RERGRFK+E+IY+RS QLTLGAL+SAV KF SL HVP+WKDERP SIR
Sbjct: 489 SKRGTVYHLXVRERGRFKTENIYLRSDQLTLGALKSAVHDKFSSLKHVPIWKDERPSSIR 310
Query: 64 GGDDLKLYRIYPVGLTQRQAL 2
GGD+LK+Y+IYP+GLT+RQAL
Sbjct: 309 GGDELKVYKIYPIGLTERQAL 247
Score = 104 bits (221), Expect = 6e-022
Identities = 48/75 (64%), Positives = 52/75 (69%)
Frame = +2 / +1
Query: 23 TDWVNSV*FQVIPTSNARWPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPP 202
T W+N V Q IPTSN WPFILPYRN++EG KF H Q S S*L *S +N L LKPP
Sbjct: 268 TYWINFVHLQFIPTSNT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPP 447
Query: 203 SLSDK*VVNCSLFGG 247
SLSD *VV CS F G
Sbjct: 448 SLSDX*VVYCSSFAG 492
Score = 104 bits (221), Expect = 6e-022
Identities = 48/78 (61%), Positives = 56/78 (71%)
Frame = -2 / -3
Query: 246 PPKREQFTTYLSEREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGHRAL 67
P K EQ+TT LSEREGGL QR F *DQ S*L E + C++NL PS+ FLYGRMKGH+
Sbjct: 491 PAKEEQYTTXLSEREGGLRQRTFI*DQIS*L*EL*SLLCTINLAPSSMFLYGRMKGHQVF 312
Query: 66 EVGMT*NYTEFTQSV*RN 13
EVGM * T+F Q V +N
Sbjct: 311 EVGMN*RCTKFIQ*VLQN 258
Score = 27.6 bits (54), Expect(2) = 6e-040
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -1 / -1
Query: 277 PKFTNRPCSCSTKK 236
PKF NR CSC +K
Sbjct: 523 PKFMNRSCSCXQQK 482
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 260,197,833
Number of Sequences: 312970
Number of extensions: 3610426
Number of successful extensions: 29794
Number of sequences better than 1.0e-020: 9
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6786673440
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)