TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCH13e05.yg.2.1
         (577 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BJ467229.1|BJ467229  BJ467229 K. Sato unpublished cDNA li...   146   1e-034
gb|CD663377.1|CD663377  UCRHV18_05bb12_b1 Drought-stressed D...   144   3e-034
gb|AJ461072.1|AJ461072  AJ461072 S00002 Hordeum vulgare subs...   111   3e-024
gb|AV833885.1|AV833885  AV833885 K. Sato unpublished cDNA li...   110   6e-024

>gb|BJ467229.1|BJ467229 BJ467229 K. Sato unpublished cDNA library, cv. Haruna Nijo
           germination shoots Hordeum vulgare subsp. vulgare cDNA
           clone bags26d08 3', mRNA sequence
          Length = 568

 Score =  146 bits (313), Expect = 1e-034
 Identities = 53/62 (85%), Positives = 58/62 (93%)
 Frame = -1 / -2

                                                                       
Query: 409 GIWINEMKKDGLDVKYENDGRILVEGCEDGYVIAHYQEPRDMMCLWDQFQKTKRGTCCKE 230
           GIWINEMKK+G DV Y+NDGRILVEGCEDGYV+AHYQEPR MMCLWD+FQKTKRGTCC E
Sbjct: 567 GIWINEMKKEGFDVTYQNDGRILVEGCEDGYVVAHYQEPRQMMCLWDKFQKTKRGTCCNE 388

             
Query: 229 *Q 224
           *+
Sbjct: 387 *E 382




 Score =  115 bits (245), Expect = 3e-025
 Identities = 51/63 (80%), Positives = 53/63 (84%)
 Frame = +3 / +2

                                                                       
Query: 222 FCYSLQHVPRFVF*N*SQRHIISLGSW**AIT*PSSHPSTKILPSFSYLTSKPSFFISLI 401
           + YSLQ VPRFVF N SQRHII LGSW* A T PSS PSTKILPSF Y+TSKPSFFISLI
Sbjct: 380 YSYSLQQVPRFVFWNLSQRHIICLGSW*WATTYPSSQPSTKILPSFWYVTSKPSFFISLI 559

              
Query: 402 HIP 410
           HIP
Sbjct: 560 HIP 568



>gb|CD663377.1|CD663377 UCRHV18_05bb12_b1 Drought-stressed Dicktoo barley epidermis cDNA
           library Hordeum vulgare subsp. vulgare cDNA clone
           UCRHV18_05bb12, mRNA sequence
          Length = 844

 Score =  144 bits (310), Expect = 3e-034
 Identities = 54/94 (57%), Positives = 68/94 (72%)
 Frame = -1 / -2

                                                                       
Query: 514 AHGPGYIVSKDIAKEVYRKHKSGELKMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVE 335
           AHGPGYI+S+DIAK V R H    L++FKLEDVAMGIWI + K+ G +V Y ND R   E
Sbjct: 561 AHGPGYIISRDIAKFVVRGHLERTLQLFKLEDVAMGIWIQQYKESGQEVNYANDERFYNE 382

                                             
Query: 334 GCEDGYVIAHYQEPRDMMCLWDQFQKTKRGTCCK 233
           GC+  YV+AHYQ PR M+CLW++ QK  +  CC+
Sbjct: 381 GCDSDYVLAHYQSPRLMLCLWEKLQKESQAVCCE 280



>gb|AJ461072.1|AJ461072 AJ461072 S00002 Hordeum vulgare subsp. vulgare cDNA clone
           S0000200064A07F1, mRNA sequence
          Length = 240

 Score =  111 bits (237), Expect = 3e-024
 Identities = 40/75 (53%), Positives = 52/75 (69%)
 Frame = -1 / +2

                                                                       
Query: 457 HKSGELKMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGYVIAHYQEPRDMMC 278
           H    L++FKLEDVAMGIWI + K+ G +V Y ND R   EGC+  YV+AHYQ PR M+C
Sbjct: 5   HLERTLQLFKLEDVAMGIWIQQYKESGQEVNYANDERFYNEGCDSDYVLAHYQSPRLMLC 184

                          
Query: 277 LWDQFQKTKRGTCCK 233
           LW++ QK  +  CC+
Sbjct: 185 LWEKLQKESQAVCCE 229



>gb|AV833885.1|AV833885 AV833885 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
           vulgare shoots germination Hordeum vulgare subsp.
           vulgare cDNA clone bags10a02, mRNA sequence
          Length = 681

 Score =  110 bits (235), Expect = 6e-024
 Identities = 41/109 (37%), Positives = 63/109 (57%)
 Frame = -1 / -3

                                                                       
Query: 565 KWYITXXXXXXXXXXXWAHGPGYIVSKDIAKEVYRKHKSGELKMFKLEDVAMGIWINEMK 386
           KW +T           +A+GPGY++S DIA+ +  +  +  L++FK+EDV+MG+W+ +  
Sbjct: 643 KWAVTYEEWPEEAYPSYANGPGYVISSDIARYIVSEFDTQTLRLFKMEDVSMGMWVEKFN 464

                                                            
Query: 385 KDGLDVKYENDGRILVEGCEDGYVIAHYQEPRDMMCLWDQFQKTKRGTC 239
                V+Y +D R    GC DGY+ AHYQ P+ M+CLW + Q      C
Sbjct: 463 ITRRPVEYRHDVRFYQAGCFDGYITAHYQSPQHMICLWRKLQSGSTHCC 317



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 268,302,786
Number of Sequences: 312970
Number of extensions: 3784952
Number of successful extensions: 43088
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 5938339260
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)