TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCG16f05.yg.2.1
         (585 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CA031681.1|CA031681  HX10N04r HX Hordeum vulgare subsp. v...   191   2e-048

>gb|CA031681.1|CA031681 HX10N04r HX Hordeum vulgare subsp. vulgare cDNA clone HX10N04
           5-PRIME, mRNA sequence
          Length = 518

 Score =  191 bits (412), Expect = 2e-048
 Identities = 74/124 (59%), Positives = 90/124 (72%)
 Frame = -1 / +2

                                                                       
Query: 387 SYNPRATASEYIIPYAKFLKSLNHPVCIGARINFQCHNEDVSERRSGVVVRISEIDPMKW 208
           S   RA ASE+I+PY KFLKSLN P CIG R   Q  +EDV+ERRSG++  I+E+DP++W
Sbjct: 50  SVQSRAAASEFIVPYWKFLKSLNRPFCIGMRFKIQYGSEDVNERRSGMITGINEVDPIRW 229

                                                                       
Query: 207 PGSKWRSLLVRWEDGAECNGQDRVSPWEIEIXXXXXXXXXXXXXXXSKRTKLCPQGNLDV 28
            GSKW+SLLVRWEDG +CN Q+R+SPWEIEI               SKRTKLCPQGNLDV
Sbjct: 230 TGSKWKSLLVRWEDGIDCNNQNRLSPWEIEIVGGSVSIAQSLSASSSKRTKLCPQGNLDV 409

               
Query: 27  PAMW 16
           P ++
Sbjct: 410 PTLY 421




 Score =  133 bits (285), Expect(2) = 1e-031
 Identities = 61/90 (67%), Positives = 65/90 (72%)
 Frame = +2 / -3

                                                                       
Query: 107 PPAISISHGDTLSWPLHSAPSSHLTSRLLHFEPGHFMGSISLMRTTTPDRLSLTSSLWH* 286
           PP ISISHGD+L W L S PSSHLTSRL HFEP H MGS SL+    PDRLSLTSSL +*
Sbjct: 330 PPTISISHGDSLFWLLQSMPSSHLTSRLFHFEPVHLMGSTSLIPVIIPDRLSLTSSLPY* 151

                                         
Query: 287 KLILAPIQTG*LRLFRNLAYGIIYSEAVAL 376
            L L PIQ G  RLFRN  YG I S+A AL
Sbjct: 150 ILNLIPIQNGRFRLFRNFQYGTINSDAAAL 61




 Score =  131 bits (281), Expect = 3e-030
 Identities = 56/105 (53%), Positives = 68/105 (64%)
 Frame = +1 / -2

                                                                       
Query: 25  WNVQISLRTQLGSFRTRCRKGMRNRDRTTSYLDFPWRYSILAITFSTVLPSYQQASPLRA 204
           W+VQI+LR Q  SFRT C + + N D   +YL+ PWR SIL IT + +LP YQQA P   
Sbjct: 412 WDVQIALRAQFSSFRTGC*QRLSNGD*AANYLNLPWR*SILVITVNAILPPYQQAFPF*T 233

                                                        
Query: 205 RPFHGIYFTNANNHSRSPFTNIFIMALKVDPCSNTDWMIEALQEL 339
            P +G+ F N  NHSRSP  NIF   L + P SNT W I+ALQEL
Sbjct: 232 SPPNGVNFVNTCNHSRSPLINIFTPILNLKPHSNTKWTIQALQEL 98




 Score =  127 bits (273), Expect = 4e-029
 Identities = 66/126 (52%), Positives = 78/126 (61%)
 Frame = -2 / +3

                                                                       
Query: 395 FTFLTIQELLLQNILFHTLSS*RASIIQSVLEQGSTFSAIMKMLVKGDLEWLFALVK*IP 216
           F  L  Q LL  N+LF T SS*RA I+  VLE G  FS  +KML++GDLE L  L K  P
Sbjct: 42  FLHLFNQGLLHPNLLFRTGSS*RA*IVHFVLE*GLRFSMGVKMLMRGDLE*LQVLTKLTP 221

                                                                       
Query: 215 *NGLARSGEACW*DGRTVLNVMAKIEYLHGKSR*LVVLSLFLIPFLHLVLKEPSCVLREI 36
             GL ++G+ACW* GR  L V+ KI+YLHG+ R*L   S  L    H VL E +C LR I
Sbjct: 222 LGGLVQNGKACW*GGRMALTVITKIDYLHGRLR*LAAQSPLLSLCQHPVLNELNCALRAI 401

                 
Query: 35  WTFQQC 18
           WT Q C
Sbjct: 402 WTSQLC 419




 Score =  116 bits (247), Expect = 1e-025
 Identities = 61/120 (50%), Positives = 80/120 (66%)
 Frame = -3 / +1

                                                                       
Query: 376 KSYCFRIYYSIR*VPEEPQSSSLYWSKDQLSVP**RC**KAIWSGCSH**NRSHEMAWLE 197
           K  C RIY S+  VPEEP+SS LYW++   SV  *RC**+AIW+   +* +  H + W +
Sbjct: 61  KGCCIRIYCSVLEVPEEPESSILYWNEV*DSVWE*RC**EAIWNDYRY*RS*PH*VDWFK 240

                                                                       
Query: 196 VEKPAGKMGGRC*M*WPR*SISMGNRDSWWFCLCFSFPFCI*F*KNQVVSSGKFGRSSNV 17
           +EKPAGK+GG  *+* P+*+ISMG+ DSW   L  S    I F* N  V SG+FGR ++V
Sbjct: 241 MEKPAGKVGGWH*L**PK*TISMGD*DSWRLSLHCSVSVSIQF*TN*TVPSGQFGRPNSV 420




 Score =  115 bits (245), Expect = 3e-025
 Identities = 57/107 (53%), Positives = 67/107 (62%)
 Frame = +3 / -1

                                                                       
Query: 18  TLLERPNFPEDTTWFF*N*MQKGNEKQRQNHQLSRFPMEILYLGHYIQHRPPILPAGFST 197
           T L RPN PE T  F *N M    E+ R + QLS+ PMEI+Y G+Y Q  PP LPAGFS 
Sbjct: 419 TELGRPNCPEGTV*FV*NWMLTETEQWRLSRQLSQSPMEIVYFGYYSQCHPPTLPAGFSI 240

                                                          
Query: 198 SSQAISWDLFH*CEQPLQIAFH*HLHYGTES*SLLQYRLDD*GSSGT 338
            +Q+  W    *     QIA H*HLH  TES*+  QY++DD GSSGT
Sbjct: 239 LNQST*WGQLR*YL*SFQIASH*HLHSHTES*TSFQYKMDDSGSSGT 99




 Score = 22.6 bits (43), Expect(2) = 1e-031
 Identities = 8/10 (80%), Positives = 9/10 (90%)
 Frame = +2 / -3

                     
Query: 26  GTSKFP*GHN 55
           GTSK P*GH+
Sbjct: 411 GTSKLP*GHS 382



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 226,598,055
Number of Sequences: 312970
Number of extensions: 3154380
Number of successful extensions: 35097
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6065589387
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)