TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.068P15F020924.3.1
(625 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF623053.2|BF623053 HVSMEa0012M22f Hordeum vulgare seedl... 275 2e-073
gb|BF628008.2|BF628008 HVSMEb0002J11f Hordeum vulgare seedl... 267 3e-071
gb|BI953372.1|BI953372 HVSMEm0012F09f Hordeum vulgare green... 226 1e-058
gb|BF621150.3|BF621150 HVSMEa0004J06f Hordeum vulgare seedl... 222 1e-057
gb|BQ763476.1|BQ763476 EBro02_SQ008_B17_R root, 3 week, hyd... 192 8e-050
gb|BI957958.1|BI957958 HVSMEn0012H20f Hordeum vulgare rachi... 177 7e-044
gb|CA014589.1|CA014589 HT11M09r HT Hordeum vulgare subsp. v... 142 2e-033
gb|BM373233.2|BM373233 EBma04_SQ003_K04_R maternal, 10 DPA,... 120 6e-027
gb|BM371229.2|BM371229 EBro04_SQ003_O11_R root, 3 week, sal... 119 1e-026
gb|BQ767981.1|BQ767981 EBro08_SQ010_J07_R root, 3 week, dro... 116 8e-026
gb|AL507983.1|AL507983 AL507983 Hordeum vulgare Barke devel... 114 4e-025
gb|BF259970.1|BF259970 HVSMEf0020M03f Hordeum vulgare seedl... 110 7e-024
gb|BF258425.1|BF258425 HVSMEf0015L01f Hordeum vulgare seedl... 103 1e-021
gb|AV923752.1|AV923752 AV923752 K. Sato unpublished cDNA li... 101 3e-021
gb|AV927277.1|AV927277 AV927277 K. Sato unpublished cDNA li... 101 3e-021
>gb|BF623053.2|BF623053 HVSMEa0012M22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012M22f, mRNA sequence
Length = 833
Score = 275 bits (595), Expect = 2e-073
Identities = 118/131 (90%), Positives = 126/131 (96%)
Frame = +1 / +2
Query: 199 HIVSRAALNMGISEIVFMVYRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGI 378
HIVSRAALNMGIS++VF+VYRN+ISLALLAPFAYFLEKKDRPPLT SLL EFF+LAL GI
Sbjct: 176 HIVSRAALNMGISKLVFIVYRNVISLALLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGI 355
Query: 379 TANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSI 558
TANQGFYLLGLYHLSPTYASAIQN VPAITFV+AAVLRLEQVDL RRHG+AKVVGTVVS+
Sbjct: 356 TANQGFYLLGLYHLSPTYASAIQNMVPAITFVLAAVLRLEQVDLGRRHGVAKVVGTVVSV 535
Query: 559 GGATVITLYKG 591
GGATVITLYKG
Sbjct: 536 GGATVITLYKG 568
Score = 214 bits (462), Expect = 3e-055
Identities = 103/158 (65%), Positives = 111/158 (70%)
Frame = -3 / -3
Query: 590 PLYRVMTVAPPMLTTVPTTLAXXXXXXXXXXXXXXTAAMTKVIAGTVFCIAEA*VGDR*Y 411
PL V+TVAPP LTTVPTTLA TAA TKV+AGT+F IA+A*VGDR Y
Sbjct: 567 PL*SVITVAPPTLTTVPTTLATPCRRPRSTCSSRSTAASTKVMAGTMFWIADA*VGDRWY 388
Query: 410 RPRR*KPWFAVIPHSASRKNSTSKEKVSGGLSFFSRK*ANGASKAREMRFLYTMNTISLM 231
P R* PWFAVIP SAS KNS S +KVSGGLSFFSRK*A GA +AREM TM T LM
Sbjct: 387 SPSR*NPWFAVIPMSASTKNSASSDKVSGGLSFFSRK*AKGARRAREMTLR*TMKTSLLM 208
Query: 230 PMLSAALETMWNPARRNCRASTPTSSFSFSGAIVAAAA 117
PMLSAA TMW PA RNC ASTP +SF+FS AA A
Sbjct: 207 PMLSAARLTMWKPASRNCSASTPMNSFTFSRCAAAAPA 94
Score = 110 bits (234), Expect(2) = 7e-033
Identities = 52/102 (50%), Positives = 63/102 (61%)
Frame = +3 / +1
Query: 177 AVPSGRVPHRLQGGAQHGHQRDRVHGVQEPHLPRLARSVRLLPREERQAATHLLFAGGVL 356
AVP+ R+PHR GGAQHGHQ+ R+H + + HLPR R +RLLPREE QAA L+ AGGVL
Sbjct: 154 AVPARRLPHRQPGGAQHGHQQARLHRLPQRHLPRPPRPLRLLPREEGQAAADLVAAGGVL 333
Query: 357 PASAVWDNCEXXXXXXXXXXXXTNLRLCDTEHRPRDHLRHGR 482
A A D+ E +LR+ D EH HLR GR
Sbjct: 334 RAGAHRDHGEPGVLPAGAVPPVADLRVRDPEHGAGHHLRAGR 459
Score = 95.5 bits (202), Expect(2) = 5e-041
Identities = 44/74 (59%), Positives = 50/74 (67%)
Frame = -2 / -2
Query: 594 RALVQGYDGGPSDAHHRAHHLGQPMPPAQVHLL*PEDSGHDEGDRGDGVLYRRGVGW*QV 415
+ALV+ G +DAHHRAHHLG P+PPAQVHLL PE G EGD VL R VG QV
Sbjct: 571 QALVERDHRGAADAHHRAHHLGHPVPPAQVHLLQPEHRGQHEGDGRHHVLDRGRVGRRQV 392
Query: 414 IQAQEVEALVRSYP 373
+Q Q+VE LVR P
Sbjct: 391 VQPQQVEPLVRRDP 350
Score = 92.3 bits (195), Expect(2) = 5e-041
Identities = 39/57 (68%), Positives = 44/57 (77%)
Frame = -2 / -2
Query: 324 RPVFLLEEVGERSEQGEGDEVPVHHEHDLADAHVERRPGDDVEPCQKELQGQHSDEQ 154
RPV LLEEVGE E+GEGD+V V E +LADAHVERRP DDVE ++ELQ QH EQ
Sbjct: 301 RPVLLLEEVGEGGEEGEGDDVAVDDEDELADAHVERRPADDVEAGEQELQRQHPHEQ 131
Score = 50.1 bits (103), Expect(2) = 7e-033
Identities = 20/29 (68%), Positives = 20/29 (68%)
Frame = +2 / +3
Query: 506 T*AGGMGWPRWWARW*ASEGPPS*PCTRA 592
T AGG GWPRWWARW*AS P TRA
Sbjct: 483 TWAGGTGWPRWWARW*ASAAPR*SRSTRA 569
>gb|BF628008.2|BF628008 HVSMEb0002J11f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0002J11f, mRNA sequence
Length = 665
Score = 267 bits (578), Expect = 3e-071
Identities = 115/131 (87%), Positives = 123/131 (93%)
Frame = +1 / +3
Query: 199 HIVSRAALNMGISEIVFMVYRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGI 378
HIVSRAALNMGIS++VF+VYRN+ISLALLAPFAYFLEKKDRPPLT SLL EFF+LAL GI
Sbjct: 96 HIVSRAALNMGISKLVFIVYRNVISLALLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGI 275
Query: 379 TANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSI 558
TANQGFYLLGLYHLSPTYASAIQN PAITFV+AA LRLEQVDL RRHG+A VVGTVVS+
Sbjct: 276 TANQGFYLLGLYHLSPTYASAIQNMEPAITFVLAAELRLEQVDLGRRHGVANVVGTVVSV 455
Query: 559 GGATVITLYKG 591
GGATVITLYKG
Sbjct: 456 GGATVITLYKG 488
Score = 217 bits (469), Expect = 4e-056
Identities = 102/158 (64%), Positives = 112/158 (70%)
Frame = -3 / -2
Query: 590 PLYRVMTVAPPMLTTVPTTLAXXXXXXXXXXXXXXTAAMTKVIAGTVFCIAEA*VGDR*Y 411
PL V+TVAPP LTTVPTTLA +AA TKV+AG++F IA+A*VGDR Y
Sbjct: 487 PL*SVITVAPPTLTTVPTTLATPCRRPRSTCSSRSSAASTKVMAGSMFWIADA*VGDRWY 308
Query: 410 RPRR*KPWFAVIPHSASRKNSTSKEKVSGGLSFFSRK*ANGASKAREMRFLYTMNTISLM 231
P R* PWFAVIP SAS KNS S +KVSGGLSFFSRK*A GA +AREM YTM T LM
Sbjct: 307 SPSR*NPWFAVIPMSASTKNSASSDKVSGGLSFFSRK*AKGARRAREMTLRYTMKTSLLM 128
Query: 230 PMLSAALETMWNPARRNCRASTPTSSFSFSGAIVAAAA 117
PMLSAA TMW PA RNC ASTP +SF+FS AA A
Sbjct: 127 PMLSAARLTMWKPASRNCSASTPMNSFTFSRCAAAAPA 14
Score = 108 bits (230), Expect(2) = 2e-031
Identities = 51/102 (50%), Positives = 62/102 (60%)
Frame = +3 / +2
Query: 177 AVPSGRVPHRLQGGAQHGHQRDRVHGVQEPHLPRLARSVRLLPREERQAATHLLFAGGVL 356
AVP+ R+PHR GGAQHGHQ+ R+H + + HLPR +RLLPREE QAA L+ AGGVL
Sbjct: 74 AVPARRLPHRQPGGAQHGHQQARLHRIPQRHLPRPPSPLRLLPREEGQAAADLVAAGGVL 253
Query: 357 PASAVWDNCEXXXXXXXXXXXXTNLRLCDTEHRPRDHLRHGR 482
A A D+ E +LR+ D EH HLR GR
Sbjct: 254 RAGAHRDHGEPGVLPAGAVPPVADLRVRDPEHGAGHHLRAGR 379
Score = 91.8 bits (194), Expect(2) = 4e-039
Identities = 43/74 (58%), Positives = 50/74 (67%)
Frame = -2 / -1
Query: 594 RALVQGYDGGPSDAHHRAHHLGQPMPPAQVHLL*PEDSGHDEGDRGDGVLYRRGVGW*QV 415
+ALV+ G +DAHHRAHH+G P+PPAQVHLL PE G EGD VL R VG QV
Sbjct: 491 QALVERDHRGAADAHHRAHHIGHPVPPAQVHLLQPELCGQHEGDGRLHVLDRGRVGRRQV 312
Query: 414 IQAQEVEALVRSYP 373
+Q Q+VE LVR P
Sbjct: 311 VQPQQVEPLVRRDP 270
Score = 89.5 bits (189), Expect(2) = 4e-039
Identities = 38/57 (66%), Positives = 44/57 (77%)
Frame = -2 / -1
Query: 324 RPVFLLEEVGERSEQGEGDEVPVHHEHDLADAHVERRPGDDVEPCQKELQGQHSDEQ 154
RPV LLEEVGE +GEGD+V V+ E +LADAHVERRP DDVE ++ELQ QH EQ
Sbjct: 221 RPVLLLEEVGEGG*EGEGDDVAVYDEDELADAHVERRPADDVEAGEQELQRQHPHEQ 51
Score = 47.3 bits (97), Expect(2) = 2e-031
Identities = 19/29 (65%), Positives = 19/29 (65%)
Frame = +2 / +1
Query: 506 T*AGGMGWPRWWARW*ASEGPPS*PCTRA 592
T AGG GWP WWARW*AS P TRA
Sbjct: 403 TWAGGTGWPMWWARW*ASAAPR*SRSTRA 489
>gb|BI953372.1|BI953372 HVSMEm0012F09f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0012F09f, mRNA sequence
Length = 831
Score = 226 bits (487), Expect = 1e-058
Identities = 98/111 (88%), Positives = 104/111 (93%)
Frame = +1 / +1
Query: 259 RNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGITANQGFYLLGLYHLSPTYAS 438
RN+ISLALLAPFAYFLEKKDRPPLT SLL EFF+LAL GITANQGFYLLG+ HLSPTYAS
Sbjct: 4 RNVISLALLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGITANQGFYLLGVCHLSPTYAS 183
Query: 439 AIQNTVPAITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSIGGATVITLYKG 591
AIQN VPAITFV+AAVLRLEQVDL RRHG+AKVVGTVVS+ GATVITLYKG
Sbjct: 184 AIQNMVPAITFVLAAVLRLEQVDLGRRHGVAKVVGTVVSVCGATVITLYKG 336
Score = 146 bits (314), Expect = 9e-035
Identities = 71/108 (65%), Positives = 78/108 (72%)
Frame = -3 / -2
Query: 590 PLYRVMTVAPPMLTTVPTTLAXXXXXXXXXXXXXXTAAMTKVIAGTVFCIAEA*VGDR*Y 411
PL V+TVAP LTTVPTTLA TAA TKV+AGT+F IA+A*VGDR +
Sbjct: 335 PL*SVITVAPQTLTTVPTTLATPCRRPRSTCSSRSTAASTKVMAGTMFWIADA*VGDRWH 156
Query: 410 RPRR*KPWFAVIPHSASRKNSTSKEKVSGGLSFFSRK*ANGASKAREM 267
P R* PWFAVIP SAS KNS S +KVSGGLSFFSRK*A GA +AREM
Sbjct: 155 TPSR*NPWFAVIPMSASTKNSASSDKVSGGLSFFSRK*AKGARRAREM 12
Score = 90.9 bits (192), Expect(2) = 2e-024
Identities = 43/74 (58%), Positives = 48/74 (64%)
Frame = -2 / -1
Query: 594 RALVQGYDGGPSDAHHRAHHLGQPMPPAQVHLL*PEDSGHDEGDRGDGVLYRRGVGW*QV 415
+ALV+ G +DAHHRAHHLG P+PPAQVHLL PE G EGD VL R VG QV
Sbjct: 339 QALVERDHCGAADAHHRAHHLGHPVPPAQVHLLQPEHRGQHEGDGRHHVLDRGRVGRRQV 160
Query: 414 IQAQEVEALVRSYP 373
Q+VE LVR P
Sbjct: 159 AHPQQVEPLVRRDP 118
Score = 40.9 bits (83), Expect(2) = 2e-024
Identities = 16/21 (76%), Positives = 18/21 (85%)
Frame = -2 / -1
Query: 324 RPVFLLEEVGERSEQGEGDEV 262
RPV LLEEVGE E+GEGD+V
Sbjct: 69 RPVLLLEEVGEGGEEGEGDDV 7
>gb|BF621150.3|BF621150 HVSMEa0004J06f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0004J06f, mRNA sequence
Length = 735
Score = 222 bits (480), Expect = 1e-057
Identities = 95/104 (91%), Positives = 99/104 (95%)
Frame = +1 / +3
Query: 280 LLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGITANQGFYLLGLYHLSPTYASAIQNTVP 459
LLAPFAYFLEKKDRPPLT SLL EFF+LAL GITANQGFYLLGLYHLSPTYASAIQN VP
Sbjct: 3 LLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGITANQGFYLLGLYHLSPTYASAIQNMVP 182
Query: 460 AITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSIGGATVITLYKG 591
AITFV+AAVLRLEQVDL RRHG+AKVVGTVVS+GGATVITLYKG
Sbjct: 183 AITFVLAAVLRLEQVDLGRRHGVAKVVGTVVSVGGATVITLYKG 314
Score = 144 bits (308), Expect = 6e-034
Identities = 69/104 (66%), Positives = 75/104 (72%)
Frame = -3 / -3
Query: 590 PLYRVMTVAPPMLTTVPTTLAXXXXXXXXXXXXXXTAAMTKVIAGTVFCIAEA*VGDR*Y 411
PL V+TVAPP LTTVPTTLA TAA TKV+AGT+F IA+A*VGDR Y
Sbjct: 313 PL*SVITVAPPTLTTVPTTLATPCRRPRSTCSSRSTAASTKVMAGTMFWIADA*VGDRWY 134
Query: 410 RPRR*KPWFAVIPHSASRKNSTSKEKVSGGLSFFSRK*ANGASK 279
P R* PWFAVIP SAS KNS S +KVSGGLSFFSRK*A GA +
Sbjct: 133 SPSR*NPWFAVIPMSASTKNSASSDKVSGGLSFFSRK*AKGARR 2
Score = 95.5 bits (202), Expect(2) = 8e-022
Identities = 44/74 (59%), Positives = 50/74 (67%)
Frame = -2 / -2
Query: 594 RALVQGYDGGPSDAHHRAHHLGQPMPPAQVHLL*PEDSGHDEGDRGDGVLYRRGVGW*QV 415
+ALV+ G +DAHHRAHHLG P+PPAQVHLL PE G EGD VL R VG QV
Sbjct: 317 QALVERDHRGAADAHHRAHHLGHPVPPAQVHLLQPEHRGQHEGDGRHHVLDRGRVGRRQV 138
Query: 414 IQAQEVEALVRSYP 373
+Q Q+VE LVR P
Sbjct: 137 VQPQQVEPLVRRDP 96
Score = 27.6 bits (54), Expect(2) = 8e-022
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = -2 / -2
Query: 324 RPVFLLEEVGERSEQ 280
RPV LLEEVGE E+
Sbjct: 47 RPVLLLEEVGEGGEE 3
>gb|BQ763476.1|BQ763476 EBro02_SQ008_B17_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_B17 5', mRNA sequence
Length = 536
Score = 192 bits (413), Expect(2) = 8e-050
Identities = 88/152 (57%), Positives = 103/152 (67%)
Frame = +1 / +3
Query: 76 RELAS*QLVIETRSAAAATMAPEXXXXXXXXXXXXXXXXXXHIVSRAALNMGISEIVFMV 255
R+LA + + A PE H+VSR ALNMGIS++VF V
Sbjct: 39 RQLALAAAMADAVDARRVCGMPEKAQLHVAMLALQFGYAGFHVVSRLALNMGISKLVFPV 218
Query: 256 YRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGITANQGFYLLGLYHLSPTYA 435
YRN+I+L LL PFAYFLEKKDRP LT V+FF LALCGITANQGFYLLGL + SPT+A
Sbjct: 219 YRNIIALFLLVPFAYFLEKKDRPRLTLGFAVQFFFLALCGITANQGFYLLGLDNTSPTFA 398
Query: 436 SAIQNTVPAITFVMAAVLRLEQVDLSRRHGLA 531
SAIQN+VPAITF MAA LR+E+V L RR G+A
Sbjct: 399 SAIQNSVPAITFGMAAALRIEKVRLDRRDGVA 494
Score = 144 bits (310), Expect = 3e-034
Identities = 74/137 (54%), Positives = 86/137 (62%)
Frame = -3 / -2
Query: 482 AAMTKVIAGTVFCIAEA*VGDR*YRPRR*KPWFAVIPHSASRKNSTSKEKVSGGLSFFSR 303
AAM KV+AGT F +AEA VGD RPRR*KPW AV+P SA +KN T+K +VS G SFFSR
Sbjct: 445 AAMPKVMAGTEFWMAEAKVGDVLSRPRR*KPWLAVMPQSARKKNWTAKPRVSLGRSFFSR 266
Query: 302 K*ANGASKAREMRFLYTMNTISLMPMLSAALETMWNPARRNCRASTPTSSFSFSGAIVAA 123
K*A G + M YT T LMPMLSA+ +T WNPA NC AS T S +FSG
Sbjct: 265 K*AKGTRRKSAMMLRYTGKTSLLMPMLSASRDTTWNPAYPNCSASIATWSCAFSGIPHTR 86
Query: 122 AADLVSITSC*LASSRT 72
A S + AS R+
Sbjct: 85 RASTASAMAAARAS*RS 35
Score = 75.8 bits (159), Expect(3) = 2e-025
Identities = 34/53 (64%), Positives = 37/53 (69%)
Frame = -2 / -1
Query: 324 RPVFLLEEVGERSEQGEGDEVPVHHEHDLADAHVERRPGDDVEPCQKELQGQH 166
RPV LLEEVGE E+ E D+V VH E LADAHVER+P VEP ELQ QH
Sbjct: 287 RPVLLLEEVGEGDEEEERDDVAVHGEDQLADAHVERQPRHHVEPGVPELQRQH 129
Score = 50.1 bits (103), Expect(3) = 2e-025
Identities = 24/44 (54%), Positives = 28/44 (63%)
Frame = -2 / -1
Query: 516 PAQVHLL*PEDSGHDEGDRGDGVLYRRGVGW*QVIQAQEVEALV 385
P + HLL PE GH EGD GVL G +V++AQEVEALV
Sbjct: 479 PVEPHLLDPERGGHAEGDGRHGVLDGGSEGGRRVVEAQEVEALV 348
Score = 27.2 bits (53), Expect(3) = 2e-025
Identities = 14/30 (46%), Positives = 16/30 (53%)
Frame = -1 / -3
Query: 409 GPGGRSPGSQLSHTALAGRTPPAKRR*VAA 320
GPGGRSPG + A RT R * +A
Sbjct: 372 GPGGRSPGWR*CRRARGRRTGRRSRG*ASA 283
Score = 24.9 bits (48), Expect(2) = 8e-050
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = +2 / +3
Query: 524 GWPRWWARW*ASEGPPS 574
G WWA W A G PS
Sbjct: 486 GVAMWWAPWRAWRGRPS 536
>gb|BI957958.1|BI957958 HVSMEn0012H20f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0012H20f, mRNA sequence
Length = 834
Score = 177 bits (380), Expect = 7e-044
Identities = 76/103 (73%), Positives = 86/103 (83%)
Frame = +1 / +1
Query: 208 SRAALNMGISEIVFMVYRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGITAN 387
S AAL GI +++F+VYRN+ISLALLAPFAYFLEKKDRPPLT SLL EFF+LAL GITAN
Sbjct: 172 SLAALGRGIXKLIFIVYRNVISLALLAPFAYFLEKKDRPPLTLSLLAEFFVLALIGITAN 351
Query: 388 QGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLRLEQVDLSR 516
QGFYLLGLYHL TYA IQN VP I FV+AAVLR+++ D R
Sbjct: 352 QGFYLLGLYHLVATYACGIQNMVPGIAFVLAAVLRVKRGDRGR 480
Score = 147 bits (316), Expect = 5e-035
Identities = 72/122 (59%), Positives = 80/122 (65%)
Frame = -3 / -2
Query: 482 AAMTKVIAGTVFCIAEA*VGDR*YRPRR*KPWFAVIPHSASRKNSTSKEKVSGGLSFFSR 303
AA TK + GT+F I A*V R Y P R* PWFAVIP SAS KNS S +KVSGGLSFFSR
Sbjct: 446 AASTKAMPGTMFWIPHA*VATRWYSPSR*NPWFAVIPMSASTKNSASSDKVSGGLSFFSR 267
Query: 302 K*ANGASKAREMRFLYTMNTISLMPMLSAALETMWNPARRNCRASTPTSSFSFSGAIVAA 123
K*A GA +AREM TM MP+ SAA + P RNC ASTP +SF+FS AA
Sbjct: 266 K*AKGARRAREMTLR*TMKMSLXMPLPSAARLPLCPPXSRNCSASTPMNSFTFSRCAAAA 87
Query: 122 AA 117
A
Sbjct: 86 PA 81
>gb|CA014589.1|CA014589 HT11M09r HT Hordeum vulgare subsp. vulgare cDNA clone HT11M09
5-PRIME, mRNA sequence
Length = 671
Score = 142 bits (305), Expect = 2e-033
Identities = 60/132 (45%), Positives = 89/132 (67%)
Frame = +1 / +1
Query: 199 HIVSRAALNMGISEIVFMVYRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGI 378
+++S+ AL+ G+S V + YRNLI+ LAP AY+ E++ +T +L++ F ++ G
Sbjct: 193 NVLSKLALDTGMSPYVLIAYRNLIAGMFLAPGAYYFERRSGMVITKQVLIQIFFSSIFGA 372
Query: 379 TANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSI 558
T NQ Y +GL +PT A A+ NT+PA+TFVMAA+L++E V L G AKV+GTVV +
Sbjct: 373 TLNQVLYFVGLKSTTPTVACALSNTLPALTFVMAAILKMETVRLKTSSGKAKVLGTVVCV 552
Query: 559 GGATVITLYKGP 594
GG+ V+ YKGP
Sbjct: 553 GGSMVMPFYKGP 588
>gb|BM373233.2|BM373233 EBma04_SQ003_K04_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ003_K04 5', mRNA sequence
Length = 577
Score = 120 bits (257), Expect = 6e-027
Identities = 52/132 (39%), Positives = 82/132 (62%)
Frame = +1 / +1
Query: 199 HIVSRAALNMGISEIVFMVYRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGI 378
+IVS A+L G+S V + YRNL++ ++ PFA EK RP +T + ++ LAL
Sbjct: 61 YIVSVASLKRGMSHYVLVTYRNLVATLVMLPFALVFEKGVRPKMTLRIFIKILGLALLEP 240
Query: 379 TANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSI 558
+Q Y +G S +++SA+ N +PA+TF+MA VLR+E++ L H AKV GT+ ++
Sbjct: 241 VLDQNLYYVGNKLTSASFSSALVNILPAVTFIMAIVLRMEKLRLRSSHSQAKVAGTICTV 420
Query: 559 GGATVITLYKGP 594
GA ++ +Y GP
Sbjct: 421 IGAVLMIMYHGP 456
>gb|BM371229.2|BM371229 EBro04_SQ003_O11_R root, 3 week, salt-stressed, cv Optic, EBro04
Hordeum vulgare subsp. vulgare cDNA clone
EBro04_SQ003_O11 5', mRNA sequence
Length = 597
Score = 119 bits (255), Expect = 1e-026
Identities = 49/128 (38%), Positives = 83/128 (64%)
Frame = +1 / +1
Query: 199 HIVSRAALNMGISEIVFMVYRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGI 378
+I++ A+L G+S V +VYRNL++ A++APFA + E+ RP +T ++ ++ LA
Sbjct: 214 YIIAVASLKRGMSHFVLVVYRNLVATAVMAPFALYFERGLRPKMTITIFIKIMGLAFLEP 393
Query: 379 TANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSI 558
+Q Y +G S +A+A+ N +PA+TFV+A +LR+E+V L H AK+ GTV+++
Sbjct: 394 VLDQNLYYMGANLTSAGFATALVNILPAVTFVLALILRMEKVRLRSLHSQAKIAGTVLTV 573
Query: 559 GGATVITL 582
GA ++ L
Sbjct: 574 AGAVLMVL 597
>gb|BQ767981.1|BQ767981 EBro08_SQ010_J07_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ010_J07 5', mRNA sequence
Length = 611
Score = 116 bits (249), Expect = 8e-026
Identities = 47/128 (36%), Positives = 82/128 (64%)
Frame = +1 / +3
Query: 199 HIVSRAALNMGISEIVFMVYRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGI 378
+I++ A+L G+S V +VYRNL++ A++ PFA + E+ RP +T ++ ++ LA
Sbjct: 228 YIIAVASLKAGMSHFVLVVYRNLVATAVMMPFAIYFERGLRPKMTITIFIKIMGLAFLEP 407
Query: 379 TANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSI 558
+Q Y +G S +A+A+ N +PA+TFV+A +LR+E+V L H AK+ GT++++
Sbjct: 408 VIDQNLYFMGAKMTSAGFATALVNILPAVTFVLALILRMEKVRLRSLHSQAKIAGTLLTV 587
Query: 559 GGATVITL 582
GA ++ L
Sbjct: 588 AGAVLMVL 611
>gb|AL507983.1|AL507983 AL507983 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY07G20V 5',
mRNA sequence
Length = 700
Score = 114 bits (244), Expect = 4e-025
Identities = 53/135 (39%), Positives = 76/135 (56%)
Frame = +1 / +3
Query: 202 IVSRAALNMGISEIVFMVYRNLISLALLAPFAYFLEKKDRPPLTFSLLVEFFLLALCGIT 381
+VS+AA N G + VF+ YR + LL P A E+K+ ++F LL++ FL AL G T
Sbjct: 141 VVSKAAFNQGFNTYVFIFYRQAAASLLLLPLAIAFERKNARAMSFRLLLKLFLFALIGTT 320
Query: 382 ANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLRLEQVDLSRRHGLAKVVGTVVSIG 561
+ Y + L S T SA N++P +TF +A +LR+E V L G+AKV G + +
Sbjct: 321 FSLNLYNVSLKLTSATVGSATSNSMPVVTFFIALLLRMEVVKLRSPSGMAKVAGVGLCLA 500
Query: 562 GATVITLYKGPAAAP 606
G VI Y GP+ P
Sbjct: 501 GVLVIAFYVGPSLNP 545
>gb|BF259970.1|BF259970 HVSMEf0020M03f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020M03f, mRNA sequence
Length = 549
Score = 110 bits (235), Expect = 7e-024
Identities = 47/95 (49%), Positives = 66/95 (69%)
Frame = +1 / +2
Query: 313 KDRPPLTFSLLVEFFLLALCGITANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLR 492
K RP +T + + FLLAL G +Q FY GL + SPT++ A+ N +PA+TFVMA + R
Sbjct: 23 KVRPRMTPLVFLNIFLLALMGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFR 202
Query: 493 LEQVDLSRRHGLAKVVGTVVSIGGATVITLYKGPA 597
+E+VDL + LAKV GT+V++ GA ++TLYKG A
Sbjct: 203 MEKVDLKKARCLAKVAGTLVTVAGAMLMTLYKGRA 307
>gb|BF258425.1|BF258425 HVSMEf0015L01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0015L01f, mRNA sequence
Length = 620
Score = 103 bits (219), Expect = 1e-021
Identities = 44/95 (46%), Positives = 64/95 (67%)
Frame = +1 / +1
Query: 313 KDRPPLTFSLLVEFFLLALCGITANQGFYLLGLYHLSPTYASAIQNTVPAITFVMAAVLR 492
K RP +T + + FLL L G +Q FY GL + PT++ A+ N +PA+TFVMA + R
Sbjct: 25 KVRPRMTHLVFLNIFLLDLMGPVIHQNFYYAGLMYTYPTFSCAMSNMLPAMTFVMADIFR 204
Query: 493 LEQVDLSRRHGLAKVVGTVVSIGGATVITLYKGPA 597
+E+VDL++ LA V GT+V++ GA ++TLYKG A
Sbjct: 205 MEKVDLTKAMCLANVAGTLVTVAGAMLMTLYKGRA 309
>gb|AV923752.1|AV923752 AV923752 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1f03 5', mRNA sequence
Length = 525
Score = 101 bits (216), Expect = 3e-021
Identities = 47/70 (67%), Positives = 50/70 (71%)
Frame = -3 / -2
Query: 326 GGLSFFSRK*ANGASKAREMRFLYTMNTISLMPMLSAALETMWNPARRNCRASTPTSSFS 147
GGLSFFSRK*A GA +AREM TM T LMPMLSAA TMW PA RNC ASTP +SF+
Sbjct: 296 GGLSFFSRK*AKGARRAREMTLR*TMKTSLLMPMLSAARLTMWKPASRNCSASTPMNSFT 117
Query: 146 FSGAIVAAAA 117
FS AA A
Sbjct: 116 FSRCAAAAPA 87
>gb|AV927277.1|AV927277 AV927277 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1f03 3', mRNA sequence
Length = 647
Score = 101 bits (216), Expect = 3e-021
Identities = 47/70 (67%), Positives = 50/70 (71%)
Frame = -3 / +3
Query: 326 GGLSFFSRK*ANGASKAREMRFLYTMNTISLMPMLSAALETMWNPARRNCRASTPTSSFS 147
GGLSFFSRK*A GA +AREM TM T LMPMLSAA TMW PA RNC ASTP +SF+
Sbjct: 414 GGLSFFSRK*AKGARRAREMTLR*TMKTSLLMPMLSAARLTMWKPASRNCSASTPMNSFT 593
Query: 146 FSGAIVAAAA 117
FS AA A
Sbjct: 594 FSRCAAAAPA 623
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 279,466,804
Number of Sequences: 312970
Number of extensions: 4239983
Number of successful extensions: 113877
Number of sequences better than 1.0e-020: 19
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6617006604
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)