TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN21e06.xg.2.1
(704 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA li... 308 2e-083
gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. v... 299 1e-080
gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. v... 273 9e-073
gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedl... 243 8e-064
gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Li... 180 3e-058
gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, n... 177 4e-048
gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Le... 168 2e-041
gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA li... 155 2e-037
gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA li... 149 2e-035
gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subs... 114 2e-029
gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. v... 108 2e-027
gb|AL499972.1|AL499972 AL499972 Hordeum vulgare Barke etiol... 84 3e-027
gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachi... 116 2e-025
gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA li... 109 2e-023
>gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 3', mRNA sequence
Length = 659
Score = 308 bits (668), Expect = 2e-083
Identities = 117/151 (77%), Positives = 135/151 (89%)
Frame = +3 / -2
Query: 33 GSPEAATLVRWICQSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFKEKPVFTFG 212
G PEAATL RWICQSYR+HLG +L +D+AGESLL M WDH+DA+LCCSFKEKPVFTF
Sbjct: 658 GFPEAATLARWICQSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFA 479
Query: 213 NQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHV 392
N+MG++MLET+ VALQDL+LDKI DE GRKAL++E+PKLMEQG+ YLP GVCLSGMGRHV
Sbjct: 478 NEMGINMLETSFVALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHV 299
Query: 393 SYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
S+E A+AWKV+GED NVHCLAFCFVNWSFV*
Sbjct: 298 SFENAIAWKVVGEDNNVHCLAFCFVNWSFV* 206
Score = 221 bits (477), Expect = 3e-057
Identities = 104/150 (69%), Positives = 110/150 (73%)
Frame = -2 / +3
Query: 481 TKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACSA 302
TKDQLTKQKARQCTL SSP TF A A S ETWRPIPD+HTP GR PCSI+FG S SA
Sbjct: 210 TKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYSA 389
Query: 301 FLPGSSEILSRVRSCRATKVVSNMSTPIWLPKVNTGFSLKEQHKTAS*WSQNIVKSDSPA 122
FLP SS ILS RS RATK VSNM PI VNTGFSLKEQHK AS SQ+I+ DSPA
Sbjct: 390 FLPASSNILSNERS*RATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSPA 569
Query: 121 *SEWLTKSAPK*YR*LWQIQRTSVAASGEP 32
S L S P+ R*LW IQR SVAASG+P
Sbjct: 570 SSACLNNSTPRWNR*LWHIQRASVAASGKP 659
Score = 195 bits (421), Expect = 2e-049
Identities = 87/151 (57%), Positives = 110/151 (72%)
Frame = -1 / +2
Query: 485 SDEGPVDETEGEAVHVAVLTENFPSNSLLVGDMAAHPRQAHPSRQVRIALFHQFRNLRM* 306
S E PVDE EG+AVH+ VLT+ FP S+L GDMAAHPRQAH + QV AL HQ +LR+*
Sbjct: 206 SHERPVDEAEGKAVHIVVLTDYFPCYSILEGDMAAHPRQAHTTWQVNKALLHQLWDLRV* 385
Query: 305 CLSTRLVRDLVKGEILQSD*SRF*HVNSHLVAESKHRLFLERAAQDSVLMVPEHCQK*FP 126
CLST ++ LV+ E+L+ + F HVN HLV + K+RLFLER AQ+S+ M+PEH Q FP
Sbjct: 386 CLSTSFIKYLVQREVLKGNERCFQHVNPHLVCKRKYRLFLERTAQNSIFMIPEHSQ*RFP 565
Query: 125 SLIRVADQIRPQVIPVALANPANKCSSFRGA 33
S I ++ + PQ+ PVALA PA+KC SFR A
Sbjct: 566 SFICLSK*LDPQMEPVALAYPASKCCSFREA 658
Score = 176 bits (378), Expect = 2e-043
Identities = 81/139 (58%), Positives = 95/139 (68%)
Frame = +1 / -3
Query: 34 APLKLLHLFAGFAKATGITWGRIWSATRIKLGNHF*QCSGTIKTLSCAALSRKSLCLLSA 213
A LKL HL AG+AKATG WG + ++KLGN +*+CSG +K L CA LSRKSL L
Sbjct: 657 ASLKLQHLLAGYAKATGSIWGSSYLDRQMKLGNLY*ECSGIMKMLFCAVLSRKSLYLRLQ 478
Query: 214 TRWELTC*KRL*SLCKISPLTRSLTSLVERHYMRRFRN*WNRAMRTCRLGCACLGWAAMS 393
TRW LTC K L K S TR L LVERHY R ++*W+RA+ TC++ CACLGWAAMS
Sbjct: 477 TRWGLTCWKHLSLPFKTSRWTRYLMKLVERHYTLRSQS*WSRALFTCQVVCACLGWAAMS 298
Query: 394 PTSKLLLGKFSVRTATCTA 450
P+ L GK SVRT CTA
Sbjct: 297 PSRML*HGK*SVRTTMCTA 241
Score = 155 bits (332), Expect(2) = 9e-046
Identities = 74/141 (52%), Positives = 90/141 (63%)
Frame = +2 / -1
Query: 122 SWGITFDNVLGPSRRCLVLLFQGKACVYFRQPDGS*HVRNDFSRSARSHP*QDL*RAW*K 301
SWGI +N LG R VL FQGKAC+Y + DG *HV N F +R QD+* +W*K
Sbjct: 569 SWGIFIENALGS*RCYFVLFFQGKACIYVCKRDGD*HVGNIFRCPSRPLVGQDI**SW*K 390
Query: 302 GTTCGGSETDGTGLCVPAGWGVPVWDGPPCLLRASCCLESSR*GRQRALPRLLFRQLVLR 481
GT + DG GLC+PA W V VWDGPPCLLR +ESSR*G+Q ALP LL RQLV R
Sbjct: 389 GTIL*DPKVDGAGLCLPARWCVLVWDGPPCLLRECYSMESSR*GQQCALPCLLLRQLVFR 210
Query: 482 LM*SKCTAETLRSASIFAFSV 544
++ + + +SIFA S+
Sbjct: 209 VIILPRQSVPVARSSIFALSL 147
Score = 48.7 bits (100), Expect(2) = 9e-046
Identities = 18/22 (81%), Positives = 19/22 (86%)
Frame = +2 / -1
Query: 32 WLP*SCYTCSLDLPKLPVSLGG 97
WLP*SC TCSLD+PKLPV GG
Sbjct: 659 WLP*SCNTCSLDMPKLPVPSGG 594
>gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. vulgare cDNA clone HI06I11
3-PRIME, mRNA sequence
Length = 638
Score = 299 bits (647), Expect = 1e-080
Identities = 113/147 (76%), Positives = 131/147 (89%)
Frame = +3 / -1
Query: 45 AATLVRWICQSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFKEKPVFTFGNQMG 224
AATL RWIC SYR+HLG +L +D+AGESLL M WDH+DA+LCCSFKEKPVFTF N+MG
Sbjct: 638 AATLARWICHSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFANEMG 459
Query: 225 VDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQ 404
++MLET+ VALQDL+LDKI DE GRKAL++E+PKLMEQG+ YLP GVCLSGMGRHVS+E
Sbjct: 458 INMLETSFVALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFEN 279
Query: 405 AVAWKVLGEDGNVHCLAFCFVNWSFV* 485
A+AWKV+GED NVHCLAFCFVNWSFV*
Sbjct: 278 AIAWKVVGEDNNVHCLAFCFVNWSFV* 198
Score = 211 bits (455), Expect = 4e-054
Identities = 100/145 (68%), Positives = 105/145 (72%)
Frame = -2 / +1
Query: 481 TKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACSA 302
TKDQLTKQKARQCTL SSP TF A A S ETWRPIPD+HTP GR PCSI+FG S SA
Sbjct: 202 TKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYSA 381
Query: 301 FLPGSSEILSRVRSCRATKVVSNMSTPIWLPKVNTGFSLKEQHKTAS*WSQNIVKSDSPA 122
FLP SS ILS RS RATK VSNM PI VNTGFSLKEQHK AS SQ+I+ DSPA
Sbjct: 382 FLPASSNILSNERS*RATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSPA 561
Query: 121 *SEWLTKSAPK*YR*LWQIQRTSVA 47
S L S P+ R*LW IQR SVA
Sbjct: 562 SSACLNNSTPRWNR*LWHIQRASVA 636
Score = 188 bits (405), Expect = 3e-047
Identities = 83/147 (56%), Positives = 107/147 (72%)
Frame = -1 / +3
Query: 485 SDEGPVDETEGEAVHVAVLTENFPSNSLLVGDMAAHPRQAHPSRQVRIALFHQFRNLRM* 306
S E PVDE EG+AVH+ VLT+ FP S+L GDMAAHPRQAH + QV AL HQ +LR+*
Sbjct: 198 SHERPVDEAEGKAVHIVVLTDYFPCYSILEGDMAAHPRQAHTTWQVNKALLHQLWDLRV* 377
Query: 305 CLSTRLVRDLVKGEILQSD*SRF*HVNSHLVAESKHRLFLERAAQDSVLMVPEHCQK*FP 126
CLST ++ LV+ E+L+ + F HVN HLV + K+RLFLER AQ+S+ M+PEH Q FP
Sbjct: 378 CLSTSFIKYLVQREVLKGNERCFQHVNPHLVCKRKYRLFLERTAQNSIFMIPEHSQ*RFP 557
Query: 125 SLIRVADQIRPQVIPVALANPANKCSS 45
S I ++ + PQ+ PVA+A PA+KC S
Sbjct: 558 SFICLSK*LDPQMEPVAVAYPASKCCS 638
Score = 167 bits (360), Expect = 5e-041
Identities = 77/135 (57%), Positives = 91/135 (67%)
Frame = +1 / -2
Query: 46 LLHLFAGFAKATGITWGRIWSATRIKLGNHF*QCSGTIKTLSCAALSRKSLCLLSATRWE 225
L HL AG+A ATG WG + ++KLGN +*+CSG +K L CA LSRKSL L TRW
Sbjct: 637 LQHLLAGYATATGSIWGSSYLDRQMKLGNLY*ECSGIMKMLFCAVLSRKSLYLRLQTRWG 458
Query: 226 LTC*KRL*SLCKISPLTRSLTSLVERHYMRRFRN*WNRAMRTCRLGCACLGWAAMSPTSK 405
LTC K L K S TR L LVERHY R ++*W+RA+ TC++ CACLGWAAMSP+
Sbjct: 457 LTCWKHLSLPFKTSRWTRYLMKLVERHYTLRSQS*WSRALFTCQVVCACLGWAAMSPSRM 278
Query: 406 LLLGKFSVRTATCTA 450
L GK SVRT CTA
Sbjct: 277 L*HGK*SVRTTMCTA 233
Score = 155 bits (332), Expect(2) = 2e-041
Identities = 74/141 (52%), Positives = 90/141 (63%)
Frame = +2 / -3
Query: 122 SWGITFDNVLGPSRRCLVLLFQGKACVYFRQPDGS*HVRNDFSRSARSHP*QDL*RAW*K 301
SWGI +N LG R VL FQGKAC+Y + DG *HV N F +R QD+* +W*K
Sbjct: 561 SWGIFIENALGS*RCYFVLFFQGKACIYVCKRDGD*HVGNIFRCPSRPLVGQDI**SW*K 382
Query: 302 GTTCGGSETDGTGLCVPAGWGVPVWDGPPCLLRASCCLESSR*GRQRALPRLLFRQLVLR 481
GT + DG GLC+PA W V VWDGPPCLLR +ESSR*G+Q ALP LL RQLV R
Sbjct: 381 GTIL*DPKVDGAGLCLPARWCVLVWDGPPCLLRECYSMESSR*GQQCALPCLLLRQLVFR 202
Query: 482 LM*SKCTAETLRSASIFAFSV 544
++ + + +SIFA S+
Sbjct: 201 VIILPRQSVPVARSSIFALSL 139
Score = 34.5 bits (69), Expect(2) = 2e-041
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +2 / -3
Query: 47 CYTCSLDLPKLPVSLGG 97
C TCSLD+P+LPV GG
Sbjct: 636 CNTCSLDMPQLPVPSGG 586
>gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. vulgare cDNA clone HX01K23
5-PRIME, mRNA sequence
Length = 633
Score = 273 bits (590), Expect = 9e-073
Identities = 102/136 (75%), Positives = 120/136 (88%)
Frame = +3 / +1
Query: 72 QSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFKEKPVFTFGNQMGVDMLETTLV 251
QSYR+HLG +L +D+AGESLL M WDH+DA+LCCSFKEKPVFTF N+MG++MLET+ V
Sbjct: 10 QSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFANEMGINMLETSFV 189
Query: 252 ALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGE 431
A QDL+LDKI DE GRKAL++E+PKLMEQG+ YLP GVCLSGMGRHVS+E A+AWKV+GE
Sbjct: 190 ATQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGE 369
Query: 432 DGNVHCLAFCFVNWSF 479
D NVHCLAFC VNWSF
Sbjct: 370 DNNVHCLAFCLVNWSF 417
Score = 188 bits (406), Expect = 2e-047
Identities = 91/136 (66%), Positives = 97/136 (71%)
Frame = -2 / -2
Query: 481 TKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACSA 302
+KDQLTKQKARQCTL SSP TF A A S ETWRPIPD+HTP GR PCSI+FG S SA
Sbjct: 419 SKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYSA 240
Query: 301 FLPGSSEILSRVRSCRATKVVSNMSTPIWLPKVNTGFSLKEQHKTAS*WSQNIVKSDSPA 122
FLP SS ILS RS ATK VSNM PI VNTGFSLKEQHK AS SQ+I+ DSPA
Sbjct: 239 FLPASSNILSNERS*VATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSPA 60
Query: 121 *SEWLTKSAPK*YR*L 74
S L S P+ R*L
Sbjct: 59 SSACLNNSTPRWNR*L 12
Score = 169 bits (364), Expect = 1e-041
Identities = 76/136 (55%), Positives = 97/136 (71%)
Frame = -1 / -1
Query: 479 EGPVDETEGEAVHVAVLTENFPSNSLLVGDMAAHPRQAHPSRQVRIALFHQFRNLRM*CL 300
E PVD EG+AVH+ VLT+ FP S+L GDMAAHPRQAH + QV AL HQ +LR+*CL
Sbjct: 417 ERPVD*AEGKAVHIVVLTDYFPCYSILEGDMAAHPRQAHTTWQVNKALLHQLWDLRV*CL 238
Query: 299 STRLVRDLVKGEILQSD*SRF*HVNSHLVAESKHRLFLERAAQDSVLMVPEHCQK*FPSL 120
ST ++ LV+ E+L + F HVN HLV + K+RLFLER AQ+S+ M+PEH Q FPS
Sbjct: 237 STSFIKYLVQREVLSGNERCFQHVNPHLVCKRKYRLFLERTAQNSIFMIPEHSQ*RFPSF 58
Query: 119 IRVADQIRPQVIPVAL 72
I ++ + PQ+ PVAL
Sbjct: 57 ICLSK*LDPQMEPVAL 10
Score = 156 bits (336), Expect = 9e-038
Identities = 72/126 (57%), Positives = 85/126 (67%)
Frame = +1 / +2
Query: 73 KATGITWGRIWSATRIKLGNHF*QCSGTIKTLSCAALSRKSLCLLSATRWELTC*KRL*S 252
KATG WG + ++KLGN +*+CSG +K L CA LSRKSL L TRW LTC K L
Sbjct: 11 KATGSIWGSSYLDRQMKLGNLY*ECSGIMKMLFCAVLSRKSLYLRLQTRWGLTCWKHLSL 190
Query: 253 LCKISPLTRSLTSLVERHYMRRFRN*WNRAMRTCRLGCACLGWAAMSPTSKLLLGKFSVR 432
K S TR L LVERHY R ++*W+RA+ TC++ CACLGWAAMSP+ L GK SVR
Sbjct: 191 PLKTSRWTRYLMKLVERHYTLRSQS*WSRALFTCQVVCACLGWAAMSPSRML*HGK*SVR 370
Query: 433 TATCTA 450
T CTA
Sbjct: 371 TTMCTA 388
Score = 154 bits (330), Expect = 6e-037
Identities = 74/144 (51%), Positives = 91/144 (63%)
Frame = +2 / +3
Query: 122 SWGITFDNVLGPSRRCLVLLFQGKACVYFRQPDGS*HVRNDFSRSARSHP*QDL*RAW*K 301
SWGI +N LG R VL FQGKAC+Y + DG *HV N F +R QD+* +W*K
Sbjct: 60 SWGIFIENALGS*RCYFVLFFQGKACIYVCKRDGD*HVGNIFRCHSRPLVGQDI**SW*K 239
Query: 302 GTTCGGSETDGTGLCVPAGWGVPVWDGPPCLLRASCCLESSR*GRQRALPRLLFRQLVLR 481
GT + DG GLC+PA W V VWDGPPCLLR +ESSR*G+Q ALP LL QLV R
Sbjct: 240 GTIL*DPKVDGAGLCLPARWCVLVWDGPPCLLRECYSMESSR*GQQCALPCLLLSQLVFR 419
Query: 482 LM*SKCTAETLRSASIFAFSVDEN 553
++ + + +SIFA S+ +N
Sbjct: 420 VIILPRQSVPVARSSIFALSLCKN 491
>gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001C22f, mRNA sequence
Length = 834
Score = 243 bits (525), Expect = 8e-064
Identities = 96/151 (63%), Positives = 118/151 (78%)
Frame = +3 / -3
Query: 33 GSPEAATLVRWICQSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFKEKPVFTFG 212
GS EA TL RWI +SYR+H GA+L+ Q+ ++ L W H D+++CCS K PVFTF
Sbjct: 724 GSLEAHTLARWIGRSYRFHTGAELLCTESQSADASLKALWQHSDSIMCCSLKAAPVFTFA 545
Query: 213 NQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHV 392
NQ G+DMLETTL+ALQD++L+KI D+ GRKAL +E PK+M+QG+AYLP GVC+S M R V
Sbjct: 544 NQAGLDMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFAYLPGGVCVSSMRRPV 365
Query: 393 SYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
SYEQAVAWKVLG+D HCLAF FVNWSFV*
Sbjct: 364 SYEQAVAWKVLGDDDAPHCLAFMFVNWSFV* 272
Score = 133 bits (284), Expect = 1e-030
Identities = 61/107 (57%), Positives = 67/107 (62%)
Frame = -2 / +3
Query: 481 TKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACSA 302
TKDQLT KARQC SSP TF ATACS +T R + D HTP GRYA PC + G S SA
Sbjct: 276 TKDQLTNMKARQCGASSSPSTFHATACSYDTGRLMLDTHTPPGRYAKPCCMILGNSEHSA 455
Query: 301 FLPGSSEILSRVRSCRATKVVSNMSTPIWLPKVNTGFSLKEQHKTAS 161
P SS I S S A VVS+MS P WL KVNTG + +EQH S
Sbjct: 456 LRPSSSRIFSSEMSWSAISVVSSMSRPAWLAKVNTGAAFREQHMIES 596
Score = 109 bits (233), Expect = 2e-023
Identities = 57/149 (38%), Positives = 83/149 (55%)
Frame = -1 / +2
Query: 479 EGPVDETEGEAVHVAVLTENFPSNSLLVGDMAAHPRQAHPSRQVRIALFHQFRNLRM*CL 300
EGPVDE EGEAV V+ ++ P + LLV H R AH + +VR L H F L L
Sbjct: 278 EGPVDEHEGEAVRRVVVAQHLPRHRLLVRHRPPHARHAHAAWEVRETLLHDFGELGAQRL 457
Query: 299 STRLVRDLVKGEILQSD*SRF*HVNSHLVAESKHRLFLERAAQDSVLMVPEHCQK*FPSL 120
+ +V DL++ ++L+ + R HV + LV E +HR L+ A D + ++P+ Q +L
Sbjct: 458 AAIVVEDLLQRDVLERNQRRLQHVQAGLVGEGEHRSCLQGTAHD*IRVLPQRFQGSVRAL 637
Query: 119 IRVADQIRPQVIPVALANPANKCSSFRGA 33
A + RP V PVA A+P K S +G+
Sbjct: 638 *LRAKEFRPGVKPVAPADPPRKRVSLKGS 724
>gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM011cD.08, mRNA sequence
Length = 1501
Score = 180 bits (388), Expect(2) = 3e-058
Identities = 68/117 (58%), Positives = 90/117 (76%)
Frame = +3 / +2
Query: 33 GSPEAATLVRWICQSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFKEKPVFTFG 212
GSPEA TL RWI +SYR+H GA+L+ Q+ ++ L W H D+++CCS K PVFTF
Sbjct: 296 GSPEAHTLARWIGRSYRFHTGAELLCTESQSADASLKALWQHSDSIMCCSLKAAPVFTFA 475
Query: 213 NQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMG 383
NQ G+DMLETTL+ALQD++L+KI D+ GRKAL +E P +M+QG+AYLP GVC+S +G
Sbjct: 476 NQAGLDMLETTLIALQDISLEKILDDDGRKALCSEFPXIMQQGFAYLPXGVCVSSIG 646
Score = 86.8 bits (183), Expect(2) = 5e-026
Identities = 39/74 (52%), Positives = 44/74 (59%)
Frame = -2 / -2
Query: 382 PIPDRHTPAGRYA*PCSISFGTSACSAFLPGSSEILSRVRSCRATKVVSNMSTPIWLPKV 203
P+ D HTP GRYA PC + G S SA P SS I S S A VVS+MS P WL KV
Sbjct: 645 PMLDTHTPXGRYAKPCCMIXGNSEHSALRPSSSRIFSSEMSWSAISVVSSMSRPAWLAKV 466
Query: 202 NTGFSLKEQHKTAS 161
NTG + +EQH S
Sbjct: 465 NTGAAFREQHMIES 424
Score = 64.8 bits (135), Expect(2) = 3e-058
Identities = 27/35 (77%), Positives = 28/35 (80%)
Frame = +3 / +3
Query: 381 GRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
GR VSYEQAVAWKVL +D HCLAF VNWSFV*
Sbjct: 645 GRPVSYEQAVAWKVLXDDDAPHCLAFHVVNWSFV* 749
Score = 50.6 bits (104), Expect(2) = 5e-026
Identities = 23/34 (67%), Positives = 24/34 (70%)
Frame = -2 / -1
Query: 481 TKDQLTKQKARQCTLPSSPRTFQATACS*ETWRP 380
TKDQLT KARQC SS TF ATACS +T RP
Sbjct: 745 TKDQLTT*KARQCGASSSXSTFHATACSYDTGRP 644
>gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ004_N18 5', mRNA sequence
Length = 540
Score = 177 bits (381), Expect(2) = 4e-048
Identities = 70/121 (57%), Positives = 89/121 (73%)
Frame = +3 / +3
Query: 72 QSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFKEKPVFTFGNQMGVDMLETTLV 251
+SYR H G ++ + +S L + W+H DA+LCCS K P+FTFGN +DMLETTLV
Sbjct: 3 RSYRAHTGEEIRWSDTEEADSPLKLLWNHSDAILCCSLKPAPMFTFGNNAALDMLETTLV 182
Query: 252 ALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGE 431
LQD++L+ I D+ GRKAL AE K+M+QG+AYLP GVC S MGR SYEQAVAWKV+G+
Sbjct: 183 NLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAVAWKVVGD 362
Query: 432 D 434
D
Sbjct: 363 D 365
Score = 111 bits (238), Expect(2) = 6e-027
Identities = 52/102 (50%), Positives = 60/102 (58%)
Frame = -2 / -3
Query: 433 SSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACSAFLPGSSEILSRVRSCR 254
SSP TF ATACS + RP+ D HTP GRYA PC ++ SA SA P SS I S SCR
Sbjct: 364 SSPTTFHATACSYDACRPMLDLHTPPGRYAKPCCMTLENSAHSALRPSSSRIASSEMSCR 185
Query: 253 ATKVVSNMSTPIWLPKVNTGFSLKEQHKTAS*WSQNIVKSDS 128
T VVS+MS LPKVN G +EQ AS W + +S
Sbjct: 184 FTSVVSSMSRAALLPKVNIGAGFREQQSMASLWFHRSFRGES 59
Score = 34.1 bits (68), Expect(2) = 4e-048
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +3 / +3
Query: 435 GNVHCLAFCFVNWSFV* 485
G HCLAF FVNW+F+*
Sbjct: 372 GAPHCLAFMFVNWTFL* 422
Score = 28.6 bits (56), Expect(2) = 6e-027
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -1 / -2
Query: 485 SDEGPVDETEGEAVHVA 435
S EGPVDE EG+AV A
Sbjct: 422 SQEGPVDEHEGKAVRRA 372
>gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 523
Score = 168 bits (362), Expect = 2e-041
Identities = 65/79 (82%), Positives = 73/79 (92%)
Frame = +3 / +3
Query: 249 VALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLG 428
VALQDL+LDKI DE GRKAL++E+PKLMEQG+ YLP GVCLSGMGRHVS+E A+AWKV+G
Sbjct: 6 VALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVG 185
Query: 429 EDGNVHCLAFCFVNWSFV* 485
ED NVHCLAFCFVNWSFV*
Sbjct: 186 EDNNVHCLAFCFVNWSFV* 242
Score = 132 bits (282), Expect = 3e-030
Identities = 59/79 (74%), Positives = 61/79 (77%)
Frame = -2 / -1
Query: 481 TKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACSA 302
TKDQLTKQKARQCTL SSP TF A A S ETWRPIPD+HTP GR PCSI+FG S SA
Sbjct: 238 TKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYSA 59
Query: 301 FLPGSSEILSRVRSCRATK 245
FLP SS ILS RS RATK
Sbjct: 58 FLPASSNILSNERS*RATK 2
Score = 107 bits (229), Expect = 5e-023
Identities = 46/79 (58%), Positives = 59/79 (74%)
Frame = -1 / -3
Query: 485 SDEGPVDETEGEAVHVAVLTENFPSNSLLVGDMAAHPRQAHPSRQVRIALFHQFRNLRM* 306
S E PVDE EG+AVH+ VLT+ FP S+L GDMAAHPRQAH + QV AL HQ +LR+*
Sbjct: 242 SHERPVDEAEGKAVHIVVLTDYFPCYSILEGDMAAHPRQAHTTWQVNKALLHQLWDLRV* 63
Query: 305 CLSTRLVRDLVKGEILQSD 249
CLST ++ LV+ E+L+ +
Sbjct: 62 CLSTSFIKYLVQREVLKGN 6
Score = 107 bits (228), Expect = 7e-023
Identities = 51/95 (53%), Positives = 63/95 (66%)
Frame = +2 / +2
Query: 275 QDL*RAW*KGTTCGGSETDGTGLCVPAGWGVPVWDGPPCLLRASCCLESSR*GRQRALPR 454
QD+* +W*KGT + DG GLC+PA W V VWDGPPCLLR +ESSR*G+Q ALP
Sbjct: 32 QDI**SW*KGTIL*DPKVDGAGLCLPARWCVLVWDGPPCLLRECYSMESSR*GQQCALPC 211
Query: 455 LLFRQLVLRLM*SKCTAETLRSASIFAFSVDENGA 559
LL RQLV R++ + + +SIFA S+ E A
Sbjct: 212 LLLRQLVFRVIILPRQSVPVARSSIFALSL*ERTA 316
>gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 3', mRNA sequence
Length = 420
Score = 155 bits (334), Expect = 2e-037
Identities = 59/72 (81%), Positives = 66/72 (91%)
Frame = +3 / -3
Query: 270 LDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGEDGNVHC 449
LDKI DE GRKAL++E+PKLMEQG+ YLP GVCLSGMGRHVS+E A+AWKV+GED NVHC
Sbjct: 418 LDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGEDNNVHC 239
Query: 450 LAFCFVNWSFV* 485
LAFCFVNWSFV*
Sbjct: 238 LAFCFVNWSFV* 203
Score = 122 bits (260), Expect = 3e-027
Identities = 53/70 (75%), Positives = 55/70 (78%)
Frame = -2 / +3
Query: 481 TKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACSA 302
TKDQLTKQKARQCTL SSP TF A A S ETWRPIPD+HTP GR PCSI+FG S SA
Sbjct: 207 TKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYSA 386
Query: 301 FLPGSSEILS 272
FLP SS ILS
Sbjct: 387 FLPASSNILS 416
Score = 105 bits (225), Expect = 2e-022
Identities = 49/90 (54%), Positives = 61/90 (67%)
Frame = +2 / -2
Query: 275 QDL*RAW*KGTTCGGSETDGTGLCVPAGWGVPVWDGPPCLLRASCCLESSR*GRQRALPR 454
QD+* +W*KGT + DG GLC+PA W V VWDGPPCLLR +ESSR*G+Q ALP
Sbjct: 413 QDI**SW*KGTIL*DPKVDGAGLCLPARWCVLVWDGPPCLLRECYSMESSR*GQQCALPC 234
Query: 455 LLFRQLVLRLM*SKCTAETLRSASIFAFSV 544
LL RQLV R++ + + +SIFA S+
Sbjct: 233 LLLRQLVFRVIILPRQSVPVARSSIFALSL 144
Score = 102 bits (217), Expect = 2e-021
Identities = 44/72 (61%), Positives = 54/72 (75%)
Frame = -1 / +2
Query: 485 SDEGPVDETEGEAVHVAVLTENFPSNSLLVGDMAAHPRQAHPSRQVRIALFHQFRNLRM* 306
S E PVDE EG+AVH+ VLT+ FP S+L GDMAAHPRQAH + QV AL HQ +LR+*
Sbjct: 203 SHERPVDEAEGKAVHIVVLTDYFPCYSILEGDMAAHPRQAHTTWQVNKALLHQLWDLRV* 382
Query: 305 CLSTRLVRDLVK 270
CLST ++ LV+
Sbjct: 383 CLSTSFIKYLVQ 418
>gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 5', mRNA sequence
Length = 447
Score = 149 bits (319), Expect = 2e-035
Identities = 56/69 (81%), Positives = 63/69 (91%)
Frame = +3 / +1
Query: 279 ISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGEDGNVHCLAF 458
I DE GRKAL++E+PKLMEQG+ YLP GVCLSGMGRHVS+E A+AWKV+GED NVHCLAF
Sbjct: 13 IFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGEDNNVHCLAF 192
Query: 459 CFVNWSFV* 485
CFVNWSFV*
Sbjct: 193 CFVNWSFV* 219
Score = 117 bits (251), Expect = 5e-026
Identities = 51/69 (73%), Positives = 53/69 (76%)
Frame = -2 / -2
Query: 481 TKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACSA 302
TKDQLTKQKARQCTL SSP TF A A S ETWRPIPD+HTP GR PCSI+FG S SA
Sbjct: 215 TKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYSA 36
Query: 301 FLPGSSEIL 275
FLP SS L
Sbjct: 35 FLPASSNXL 9
Score = 101 bits (215), Expect = 5e-021
Identities = 46/85 (54%), Positives = 57/85 (67%)
Frame = +2 / +3
Query: 290 AW*KGTTCGGSETDGTGLCVPAGWGVPVWDGPPCLLRASCCLESSR*GRQRALPRLLFRQ 469
+W*KGT + DG GLC+PA W V VWDGPPCLLR +ESSR*G+Q ALP LL RQ
Sbjct: 24 SW*KGTIL*DPKVDGAGLCLPARWCVLVWDGPPCLLRECYSMESSR*GQQCALPCLLLRQ 203
Query: 470 LVLRLM*SKCTAETLRSASIFAFSV 544
LV R++ + + +SIFA S+
Sbjct: 204 LVFRVIILPRQSVPVARSSIFALSL 278
>gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200065F09F1, mRNA sequence
Length = 360
Score = 114 bits (244), Expect(2) = 2e-029
Identities = 47/68 (69%), Positives = 56/68 (82%)
Frame = +3 / +3
Query: 231 MLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAV 410
MLETTLV LQD++L+ I D+ GRKAL AE K+M+QG+AYLP GVC S MGR SYEQAV
Sbjct: 3 MLETTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAV 182
Query: 411 AWKVLGED 434
AWKV+G+D
Sbjct: 183 AWKVVGDD 206
Score = 34.1 bits (68), Expect(2) = 2e-029
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +3 / +3
Query: 435 GNVHCLAFCFVNWSFV* 485
G HCLAF FVNW+F+*
Sbjct: 213 GAPHCLAFMFVNWTFL* 263
>gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. vulgare cDNA clone HX01B05
3-PRIME, mRNA sequence
Length = 489
Score = 108 bits (230), Expect(2) = 2e-027
Identities = 44/65 (67%), Positives = 53/65 (81%)
Frame = +3 / -1
Query: 240 TTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAVAWK 419
TTLV LQD++L+ I D+ GRKAL AE K+M+QG+AYLP GVC S MGR SYEQAVAWK
Sbjct: 489 TTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAVAWK 310
Query: 420 VLGED 434
V+G+D
Sbjct: 309 VVGDD 295
Score = 34.1 bits (68), Expect(2) = 2e-027
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +3 / -1
Query: 435 GNVHCLAFCFVNWSFV* 485
G HCLAF FVNW+F+*
Sbjct: 288 GAPHCLAFMFVNWTFL* 238
>gb|AL499972.1|AL499972 AL499972 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK04F09r 3', mRNA sequence
Length = 573
Score = 84.5 bits (178), Expect(3) = 3e-027
Identities = 31/37 (83%), Positives = 34/37 (91%)
Frame = +3 / -3
Query: 375 GMGRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
GMGRHVS+E A+AWKV+GED NV CLAFCFVNWSFV*
Sbjct: 364 GMGRHVSFENAIAWKVVGEDNNVXCLAFCFVNWSFV* 254
Score = 46.0 bits (94), Expect(3) = 3e-027
Identities = 17/23 (73%), Positives = 19/23 (82%)
Frame = +3 / -2
Query: 315 EVPKLMEQGYAYLPAGVCLSGMG 383
E+PKLMEQG+ YLP GVCLS G
Sbjct: 425 EIPKLMEQGFVYLPGGVCLSWDG 357
Score = 29.0 bits (57), Expect(3) = 3e-027
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +3 / -2
Query: 222 GVDMLETTLVALQDLTLDKISDE 290
G+ + VALQDL+LDKI DE
Sbjct: 521 GLTCWKHLFVALQDLSLDKIFDE 453
>gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009M03f, mRNA sequence
Length = 718
Score = 116 bits (247), Expect = 2e-025
Identities = 48/68 (70%), Positives = 58/68 (85%)
Frame = +3 / +1
Query: 228 DMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQA 407
DMLETTL+ALQD++L+KI D+ GRKAL +E PK+M+QG+AYLP GVC+S MGR VSYE A
Sbjct: 25 DMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFAYLPGGVCVSSMGRPVSYEXA 204
Query: 408 VAWKVLGE 431
VA KVL E
Sbjct: 205 VAXKVLAE 228
>gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 5', mRNA sequence
Length = 633
Score = 109 bits (232), Expect = 2e-023
Identities = 40/56 (71%), Positives = 47/56 (83%)
Frame = +3 / +1
Query: 12 AGQKLISGSPEAATLVRWICQSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCC 179
A QK+ISG PEAATL RWICQSYR+HLG +L +D+AGESLL M WDH+DA+LCC
Sbjct: 466 AEQKIISGFPEAATLARWICQSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCC 633
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 353,381,665
Number of Sequences: 312970
Number of extensions: 6063675
Number of successful extensions: 56072
Number of sequences better than 1.0e-020: 20
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7762257747
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)