TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN10e11.xg.2.4
(664 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU990099.1|BU990099 HF24B10r HF Hordeum vulgare subsp. v... 94 9e-037
gb|BQ472834.1|BQ472834 HB01F10r BC Hordeum vulgare subsp. v... 94 9e-037
gb|CA028533.1|CA028533 HZ62F08r HZ Hordeum vulgare subsp. v... 92 3e-036
gb|BU975772.1|BU975772 HB32E04r BC Hordeum vulgare subsp. v... 94 4e-035
gb|BU971669.1|BU971669 HB19E23r BC Hordeum vulgare subsp. v... 94 3e-033
gb|BU974041.1|BU974041 HB26L16r BC Hordeum vulgare subsp. v... 94 2e-032
gb|AV932909.1|AV932909 AV932909 K. Sato unpublished cDNA li... 68 3e-029
gb|BU989178.1|BU989178 HF21A09r HF Hordeum vulgare subsp. v... 94 2e-027
gb|AV932668.1|AV932668 AV932668 K. Sato unpublished cDNA li... 68 3e-025
gb|BJ472039.1|BJ472039 BJ472039 K. Sato unpublished cDNA li... 68 2e-024
gb|AV917169.1|AV917169 AV917169 K. Sato unpublished cDNA li... 68 6e-022
>gb|BU990099.1|BU990099 HF24B10r HF Hordeum vulgare subsp. vulgare cDNA clone HF24B10
5-PRIME, mRNA sequence
Length = 592
Score = 94.1 bits (199), Expect(3) = 9e-037
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 314 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 466
Score = 67.5 bits (141), Expect(3) = 9e-037
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +2
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 191 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 313
Score = 29.9 bits (59), Expect(3) = 9e-037
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +2
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 122 SSTSVTGLNNNDYQITRGK 178
>gb|BQ472834.1|BQ472834 HB01F10r BC Hordeum vulgare subsp. vulgare cDNA clone HB01F10
5-PRIME, mRNA sequence
Length = 404
Score = 94.1 bits (199), Expect(3) = 9e-037
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 218 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 370
Score = 67.5 bits (141), Expect(3) = 9e-037
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +2
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 95 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 217
Score = 29.9 bits (59), Expect(3) = 9e-037
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +2
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 26 SSTSVTGLNNNDYQITRGK 82
>gb|CA028533.1|CA028533 HZ62F08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ62F08
5-PRIME, mRNA sequence
Length = 344
Score = 91.8 bits (194), Expect(3) = 3e-036
Identities = 35/50 (70%), Positives = 40/50 (80%)
Frame = -2 / +1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDI 4
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDI
Sbjct: 193 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDI 342
Score = 67.5 bits (141), Expect(3) = 3e-036
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +1
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 70 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 192
Score = 29.9 bits (59), Expect(3) = 3e-036
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +1
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 1 SSTSVTGLNNNDYQITRGK 57
>gb|BU975772.1|BU975772 HB32E04r BC Hordeum vulgare subsp. vulgare cDNA clone HB32E04
5-PRIME, mRNA sequence
Length = 521
Score = 94.1 bits (199), Expect(3) = 4e-035
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 187 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 339
Score = 67.5 bits (141), Expect(3) = 4e-035
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +1
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 64 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 186
Score = 24.4 bits (47), Expect(3) = 4e-035
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2 / +1
Query: 495 GLXXNDYHVSRGK 457
GL NDY ++RGK
Sbjct: 13 GLNNNDYQITRGK 51
>gb|BU971669.1|BU971669 HB19E23r BC Hordeum vulgare subsp. vulgare cDNA clone HB19E23
5-PRIME, mRNA sequence
Length = 520
Score = 94.1 bits (199), Expect(2) = 3e-033
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 152 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 304
Score = 67.5 bits (141), Expect(2) = 3e-033
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +2
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 29 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 151
>gb|BU974041.1|BU974041 HB26L16r BC Hordeum vulgare subsp. vulgare cDNA clone HB26L16
5-PRIME, mRNA sequence
Length = 318
Score = 94.1 bits (199), Expect(2) = 2e-032
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +3
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 129 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 281
Score = 64.3 bits (134), Expect(2) = 2e-032
Identities = 24/39 (61%), Positives = 30/39 (76%)
Frame = -2 / +3
Query: 360 GLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
GL+ C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 12 GLTGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 128
>gb|AV932909.1|AV932909 AV932909 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal4j24 5', mRNA sequence
Length = 677
Score = 68.4 bits (143), Expect(3) = 3e-029
Identities = 24/34 (70%), Positives = 28/34 (82%)
Frame = -2 / +3
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKH 52
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H
Sbjct: 576 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATH 677
Score = 67.5 bits (141), Expect(3) = 3e-029
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +3
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 453 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 575
Score = 29.9 bits (59), Expect(3) = 3e-029
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +3
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 384 SSTSVTGLNNNDYQITRGK 440
>gb|BU989178.1|BU989178 HF21A09r HF Hordeum vulgare subsp. vulgare cDNA clone HF21A09
5-PRIME, mRNA sequence
Length = 576
Score = 94.1 bits (199), Expect(2) = 2e-027
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +3
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 87 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 239
Score = 48.3 bits (99), Expect(2) = 2e-027
Identities = 17/24 (70%), Positives = 21/24 (87%)
Frame = -2 / +3
Query: 315 MPIADNTFDAAYAIQATCYAPEAV 244
MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 15 MPFSDNTFDAVYAIEATCHAPDPV 86
>gb|AV932668.1|AV932668 AV932668 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal1j14 5', mRNA sequence
Length = 580
Score = 67.5 bits (141), Expect(3) = 3e-025
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +2
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 374 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 496
Score = 54.7 bits (113), Expect(3) = 3e-025
Identities = 19/28 (67%), Positives = 23/28 (82%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFD 70
G Y E+YRVLKPGQ FA+ EWC+TD +D
Sbjct: 497 GCYKEIYRVLKPGQCFAVYEWCITDHYD 580
Score = 29.9 bits (59), Expect(3) = 3e-025
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +2
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 305 SSTSVTGLNNNDYQITRGK 361
>gb|BJ472039.1|BJ472039 BJ472039 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal31k11 5', mRNA sequence
Length = 628
Score = 67.5 bits (141), Expect(3) = 2e-024
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +1
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 427 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 549
Score = 51.9 bits (107), Expect(3) = 2e-024
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = -2 / +1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTD 79
G Y E+YRVLKPGQ FA+ EWC+TD
Sbjct: 550 GCYKEIYRVLKPGQCFAVYEWCITD 624
Score = 29.9 bits (59), Expect(3) = 2e-024
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +1
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 358 SSTSVTGLNNNDYQITRGK 414
>gb|AV917169.1|AV917169 AV917169 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8c03 5', mRNA sequence
Length = 503
Score = 67.5 bits (141), Expect(2) = 6e-022
Identities = 26/41 (63%), Positives = 31/41 (75%)
Frame = -2 / +3
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GLS C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 276 SVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 398
Score = 56.1 bits (116), Expect(2) = 6e-022
Identities = 20/27 (74%), Positives = 23/27 (85%)
Frame = -2 / +1
Query: 132 RVLKPGQYFALDEWCLTDLFDPNNAKH 52
RVLKPGQ FA+ EWC+TD +DPNNA H
Sbjct: 421 RVLKPGQCFAVYEWCITDHYDPNNATH 501
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 243,108,236
Number of Sequences: 312970
Number of extensions: 3496182
Number of successful extensions: 29814
Number of sequences better than 1.0e-020: 12
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7168423821
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)