TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL6b07.xg.2.1
(644 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ464898.1|BQ464898 HU02B07r HU Hordeum vulgare subsp. v... 259 1e-075
gb|BQ466575.1|BQ466575 HT02O23r HT Hordeum vulgare subsp. v... 241 9e-063
gb|AV836884.1|AV836884 AV836884 K. Sato unpublished cDNA li... 199 4e-050
gb|AV929541.1|AV929541 AV929541 K. Sato unpublished cDNA li... 133 1e-040
gb|AV929540.1|AV929540 AV929540 K. Sato unpublished cDNA li... 126 2e-038
gb|BU983256.1|BU983256 HA29B10r HA Hordeum vulgare subsp. v... 142 1e-035
gb|CD662706.1|CD662706 UCRHV18_02dd10_b1 Drought-stressed D... 133 6e-035
gb|CB879642.1|CB879642 HP03C03w HP Hordeum vulgare subsp. v... 89 8e-030
gb|BQ470048.1|BQ470048 HX01J23T HX Hordeum vulgare subsp. v... 117 7e-029
gb|BM372335.2|BM372335 EBro03_SQ004_K20_R root, 3 week, wat... 110 4e-028
gb|BF266777.2|BF266777 HV_CEa0016A13f Hordeum vulgare seedl... 100 4e-028
gb|BG414636.2|BG414636 HVSMEk0003C21f Hordeum vulgare testa... 111 7e-028
gb|BI952328.1|BI952328 HVSMEm0005P11f Hordeum vulgare green... 124 7e-028
gb|AV917322.1|AV917322 AV917322 K. Sato unpublished cDNA li... 112 1e-027
gb|AV916932.1|AV916932 AV916932 K. Sato unpublished cDNA li... 112 1e-027
gb|AW982539.2|AW982539 HVSMEg0003I16f Hordeum vulgare pre-a... 110 2e-027
gb|BI780740.2|BI780740 EBes01_SQ002_A07_R embryo sac, 4-6 D... 92 8e-027
gb|BU967283.1|BU967283 HB03N04r BC Hordeum vulgare subsp. v... 92 8e-027
gb|BU966755.1|BU966755 HB02E05r BC Hordeum vulgare subsp. v... 92 8e-027
gb|BF266815.2|BF266815 HV_CEa0016C07f Hordeum vulgare seedl... 93 1e-026
gb|BF261904.2|BF261904 HV_CEa0002I16f Hordeum vulgare seedl... 109 1e-026
gb|BJ450289.1|BJ450289 BJ450289 K. Sato unpublished cDNA li... 112 1e-026
gb|AV833754.1|AV833754 AV833754 K. Sato unpublished cDNA li... 113 1e-026
gb|CK123771.1|CK123771 BES1824105f02 BES1824 Hordeum vulgar... 72 8e-026
gb|BI952975.1|BI952975 HVSMEm0008M21f Hordeum vulgare green... 101 9e-026
gb|AW983189.3|AW983189 HVSMEg0008J16f Hordeum vulgare pre-a... 88 1e-025
gb|CB878070.1|CB878070 HP07D18T HP Hordeum vulgare subsp. v... 117 1e-025
gb|BE060160.1|BE060160 HVSMEg0011B12f Hordeum vulgare pre-a... 102 1e-025
gb|BG367446.2|BG367446 HVSMEi0012E18f Hordeum vulgare 20 DA... 116 2e-025
gb|BU986512.1|BU986512 HF12B10r HF Hordeum vulgare subsp. v... 76 2e-025
gb|BJ464341.1|BJ464341 BJ464341 K. Sato unpublished cDNA li... 77 2e-025
gb|BQ768749.1|BQ768749 EBro08_SQ012_G15_R root, 3 week, dro... 67 3e-025
gb|AV913572.1|AV913572 AV913572 K. Sato unpublished cDNA li... 107 3e-025
gb|CA032771.1|CA032771 HX14C15r HX Hordeum vulgare subsp. v... 105 6e-025
gb|BU972150.1|BU972150 HB20M05r BC Hordeum vulgare subsp. v... 105 8e-025
gb|AV916201.1|AV916201 AV916201 K. Sato unpublished cDNA li... 84 8e-025
gb|BU971791.1|BU971791 HB19L01r BC Hordeum vulgare subsp. v... 105 8e-025
gb|CA021794.1|CA021794 HZ41C07r HZ Hordeum vulgare subsp. v... 101 8e-025
gb|BI777999.2|BI777999 EBro07_SQ001_M06_R root, 3 week, red... 100 1e-024
gb|AV833368.1|AV833368 AV833368 K. Sato unpublished cDNA li... 72 1e-024
gb|AV932936.1|AV932936 AV932936 K. Sato unpublished cDNA li... 89 3e-024
gb|BQ760957.1|BQ760957 EBro03_SQ003_P10_R root, 3 week, wat... 100 3e-024
gb|BU971391.1|BU971391 HB17H15r BC Hordeum vulgare subsp. v... 107 3e-024
gb|BJ471888.1|BJ471888 BJ471888 K. Sato unpublished cDNA li... 89 3e-024
gb|BQ764108.1|BQ764108 EBro03_SQ006_E12_R root, 3 week, wat... 83 3e-024
gb|BF261155.2|BF261155 HVSMEf0023O11f Hordeum vulgare seedl... 72 4e-024
gb|BF256987.2|BF256987 HVSMEf0011I21f Hordeum vulgare seedl... 111 7e-024
gb|BE196273.3|BE196273 HVSMEh0091N12f Hordeum vulgare 5-45 ... 95 7e-024
gb|BJ457712.1|BJ457712 BJ457712 K. Sato unpublished cDNA li... 111 9e-024
gb|CA014211.1|CA014211 HT10L08r HT Hordeum vulgare subsp. v... 111 9e-024
gb|BQ758677.1|BQ758677 EBma07_SQ002_B13_R maternal, 21 DPA,... 100 1e-023
gb|AV927561.1|AV927561 AV927561 K. Sato unpublished cDNA li... 69 1e-023
gb|BE558923.1|BE558923 HV_CEb0020M17f Hordeum vulgare seedl... 100 2e-023
gb|CA029588.1|CA029588 HZ65K08r HZ Hordeum vulgare subsp. v... 95 4e-023
gb|AV836793.1|AV836793 AV836793 K. Sato unpublished cDNA li... 108 4e-023
gb|BM816134.1|BM816134 HC05B11_T3.ab1 HC Hordeum vulgare su... 100 5e-023
gb|BI954047.1|BI954047 HVSMEm0015P18f Hordeum vulgare green... 73 1e-022
gb|BJ447136.1|BJ447136 BJ447136 K. Sato unpublished cDNA li... 72 2e-022
gb|BJ446879.1|BJ446879 BJ446879 K. Sato unpublished cDNA li... 72 2e-022
gb|BF258327.3|BF258327 HVSMEf0015G22f Hordeum vulgare seedl... 95 2e-022
gb|BM816135.1|BM816135 HC05B12_T3.ab1 HC Hordeum vulgare su... 100 2e-022
gb|AL450670.1|AL450670 AL450670 Hordeum vulgare Barke etiol... 89 3e-022
gb|BU980696.1|BU980696 HA21G18r HA Hordeum vulgare subsp. v... 105 3e-022
gb|BU980799.1|BU980799 HA21L17r HA Hordeum vulgare subsp. v... 105 3e-022
gb|BU985927.1|BU985927 HF09B02r HF Hordeum vulgare subsp. v... 98 3e-022
gb|BE558792.2|BE558792 HV_CEb0020G08f Hordeum vulgare seedl... 58 4e-022
gb|BU989313.1|BU989313 HF21G08r HF Hordeum vulgare subsp. v... 105 6e-022
gb|BJ462905.1|BJ462905 BJ462905 K. Sato unpublished cDNA li... 70 6e-022
gb|BJ469098.1|BJ469098 BJ469098 K. Sato unpublished cDNA li... 61 6e-022
dbj|AB109213.1| Hordeum vulgare BRI1 gene for putative bras... 65 7e-022
dbj|AB109215.1| Hordeum vulgare BRI1 gene for putative bras... 65 7e-022
gb|BG414953.2|BG414953 HVSMEk0004G13f Hordeum vulgare testa... 95 8e-022
gb|BF620215.2|BF620215 HVSMEc0019A20f Hordeum vulgare seedl... 63 8e-022
gb|BQ764974.1|BQ764974 EBca01_SQ005_M12_R carpel, pre-anthe... 63 8e-022
gb|BU987697.1|BU987697 HF15I03r HF Hordeum vulgare subsp. v... 63 8e-022
gb|CB877039.1|CB877039 HP03F15T HP Hordeum vulgare subsp. v... 63 8e-022
gb|AV917399.1|AV917399 AV917399 K. Sato unpublished cDNA li... 63 8e-022
gb|BU974897.1|BU974897 HB29H02r BC Hordeum vulgare subsp. v... 64 1e-021
gb|AV924450.1|AV924450 AV924450 K. Sato unpublished cDNA li... 103 1e-021
gb|BI953661.1|BI953661 HVSMEm0013M10f Hordeum vulgare green... 66 1e-021
gb|BJ462781.1|BJ462781 BJ462781 K. Sato unpublished cDNA li... 69 1e-021
gb|BI953631.1|BI953631 HVSMEm0013K08f Hordeum vulgare green... 65 2e-021
gb|BJ452111.1|BJ452111 BJ452111 K. Sato unpublished cDNA li... 69 2e-021
gb|BQ470244.1|BQ470244 HX02D24r HX Hordeum vulgare subsp. v... 66 2e-021
gb|BF618611.2|BF618611 HVSMEc0006K17f Hordeum vulgare seedl... 62 2e-021
gb|AV833420.1|AV833420 AV833420 K. Sato unpublished cDNA li... 68 3e-021
gb|BU986612.1|BU986612 HF12G11r HF Hordeum vulgare subsp. v... 53 5e-021
gb|BG344214.1|BG344214 HVSMEg0008C18f Hordeum vulgare pre-a... 90 5e-021
gb|BE603119.2|BE603119 HVSMEh0102B06f Hordeum vulgare 5-45 ... 60 7e-021
gb|BF260175.2|BF260175 HVSMEf0021E22f Hordeum vulgare seedl... 70 7e-021
gb|BJ470520.1|BJ470520 BJ470520 K. Sato unpublished cDNA li... 89 7e-021
gb|BU971659.1|BU971659 HB19E12r BC Hordeum vulgare subsp. v... 57 9e-021
gb|AV916577.1|AV916577 AV916577 K. Sato unpublished cDNA li... 87 9e-021
>gb|BQ464898.1|BQ464898 HU02B07r HU Hordeum vulgare subsp. vulgare cDNA clone HU02B07
5-PRIME, mRNA sequence
Length = 613
Score = 259 bits (561), Expect(2) = 1e-075
Identities = 106/156 (67%), Positives = 128/156 (82%)
Frame = +2 / +2
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
I+HRDVKTNNILLD FHVKVADFGLSRL+P + THVST PQGTPGYVDP YHQCY+LT+
Sbjct: 131 IVHRDVKTNNILLDTDFHVKVADFGLSRLFPLDATHVSTAPQGTPGYVDPEYHQCYQLTD 310
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYETDGE 448
KSDVYSFGVVLVEL+SSKPAVD+ R +EINLA MA+N+IQ ++ +LVD ELG+E+D
Sbjct: 311 KSDVYSFGVVLVELISSKPAVDITRQRNEINLAGMAINKIQKCQIEELVDLELGFESDPA 490
Query: 449 TKKSIDLVAELAFRCLQLERDSRPSMXXVVEALNRI 556
T+K + +VAELAFRCLQ + RP + V++ L I
Sbjct: 491 TRKMMTMVAELAFRCLQQNGEMRPPIREVLDVLRAI 598
Score = 159 bits (342), Expect(2) = 4e-042
Identities = 71/113 (62%), Positives = 81/113 (71%)
Frame = -3 / -2
Query: 387 ILLRAMLARLISLWLLPMSTAGLEDTSSTSTTPKLYTSLFSVSL*HWWYTGST*PGVPCG 208
ILL AM ARLIS MSTAGL++ SSTSTTPKLYTSL SVS *HWWY+GST PGVP G
Sbjct: 429 ILLMAMPARLISFLCRVMSTAGLDEMSSTSTTPKLYTSLLSVSW*HWWYSGSTYPGVPWG 250
Query: 207 TVDTWVTSGGYRRDSPKSATFTWKLLSSSMLLVFTSRCMISTAWRYASASAVS 49
V+TWV S G R+ PKSAT TWK +S MLLV TSRC + + + SA +
Sbjct: 249 AVETWVASSGKSRERPKSATLTWKSVSRRMLLVLTSRCTMGGSMAWR*VSAAA 91
Score = 105 bits (223), Expect(2) = 6e-025
Identities = 63/158 (39%), Positives = 77/158 (48%)
Frame = -2 / -1
Query: 562 VXDTVQGLHHXXHRRP*IPF*LQAPERQLSDEIYGFLRLTVSLVPELRVDQLAHLVVLDS 383
+ D + + H RR + LQAPE QL + R V L EL+V QL L +LD
Sbjct: 604 ILDGPEHVQHLPDRRSHLAVLLQAPEGQLRHHGHHLPRRRVRLEAELQVHQLLDLALLDL 425
Query: 382 VESHVGQVDLAVAPAHVDGWLRGHELDQHDAEXXXXXXXXXXXXXXVHRVDVARRALWDG 203
V+ H GQVDL P V G L EL QH AE V RV V AL G
Sbjct: 424 VDGHAGQVDLVPLPGDVHGGLGRDELHQHHAEAVHVALVRQLVALVVLRVHVPWGALGGG 245
Query: 202 RHVGDFRRVQARQPKVGDLHVEAVVQQYVVGLHVTVYD 89
VG R + + +VGDL VE V++ VVGL V V+D
Sbjct: 244 GDVGGVEREEPGEAEVGDLDVEVGVEEDVVGLDVAVHD 131
Score = 78.5 bits (165), Expect(3) = 4e-024
Identities = 32/62 (51%), Positives = 40/62 (64%)
Frame = +1 / +1
Query: 79 RGRDHTP*REDQQHTAGQQLPREGRRLWAVAPVPS*SHPRVDRPTGHAGLRRPGVPPVLQ 258
R P R+DQQH +LPR+GRRL + +P+ HPR+ RP GH +R PGVPPVL
Sbjct: 121 RAAHRAPRRQDQQHPPRHRLPRQGRRLRPLPALPARRHPRLHRPPGHPRVRGPGVPPVLP 300
Query: 259 AD 264
AD
Sbjct: 301 AD 306
Score = 46.9 bits (96), Expect(3) = 4e-024
Identities = 20/37 (54%), Positives = 22/37 (59%)
Frame = +3 / +3
Query: 342 GATARSTWPTWLSTESRTTRWASWSTLSSGTRLTVRR 452
G RSTWP W ST SR+ R SW T SS + T RR
Sbjct: 384 GRGTRSTWPAWPSTRSRSARSRSWWTWSSASSRTRRR 494
Score = 45.5 bits (93), Expect(2) = 1e-075
Identities = 15/28 (53%), Positives = 23/28 (82%)
Frame = +2 / +2
Query: 5 SERGLTWPRRMSIAIETAEALAYLHAVE 88
++R L WP R+++A+E+A AL YLHA+E
Sbjct: 41 ADRALPWPLRLNVAVESAAALTYLHAIE 124
Score = 31.8 bits (63), Expect(2) = 4e-042
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = -3 / -2
Query: 540 STTXXIDGRESLSSCKHRNASSAT 469
S+T I GR S C+HR ASSAT
Sbjct: 582 SSTSLIGGRISPFCCRHRKASSAT 511
Score = 28.6 bits (56), Expect(2) = 6e-025
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -2 / -1
Query: 88 LDRVEVRQRLSCFYGDAHPSRPREAALR 5
LD VEV +R GD RPR+ A+R
Sbjct: 124 LDGVEVGERRRGLDGDIQSERPRQGAVR 41
Score = 23.1 bits (44), Expect(3) = 4e-024
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = +3 / +3
Query: 477 SWRSGACS*KGIHGRR*XKWW 539
SW SGACS RR + W
Sbjct: 519 SWPSGACSRTARCDRRSGRCW 581
>gb|BQ466575.1|BQ466575 HT02O23r HT Hordeum vulgare subsp. vulgare cDNA clone HT02O23
5-PRIME, mRNA sequence
Length = 598
Score = 241 bits (521), Expect = 9e-063
Identities = 99/148 (66%), Positives = 120/148 (81%)
Frame = +2 / +1
Query: 113 NNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFG 292
NNILLD FHVKVADFGLSRL+P + THVST PQGTPGYVDP YHQCY+LT+KSDVYSFG
Sbjct: 10 NNILLDTDFHVKVADFGLSRLFPLDATHVSTAPQGTPGYVDPEYHQCYQLTDKSDVYSFG 189
Query: 293 VVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYETDGETKKSIDLV 472
VVLVEL+SSKPAVD+ R +EINLA MA+N+IQ ++ +LVD ELG+E+D T+K + +V
Sbjct: 190 VVLVELISSKPAVDITRQRNEINLAGMAINKIQKCQIEELVDLELGFESDPATRKMMTMV 369
Query: 473 AELAFRCLQLERDSRPSMXXVVEALNRI 556
AELAFRCLQ + RP + V++ L I
Sbjct: 370 AELAFRCLQQNGEMRPPIREVLDVLRAI 453
Score = 144 bits (309), Expect(2) = 1e-037
Identities = 63/93 (67%), Positives = 70/93 (75%)
Frame = -3 / -3
Query: 387 ILLRAMLARLISLWLLPMSTAGLEDTSSTSTTPKLYTSLFSVSL*HWWYTGST*PGVPCG 208
ILL AM ARLIS MSTAGL++ SSTSTTPKLYTSL SVS *HWWY+GST PGVP G
Sbjct: 284 ILLMAMPARLISFLCRVMSTAGLDEMSSTSTTPKLYTSLLSVSW*HWWYSGSTYPGVPWG 105
Query: 207 TVDTWVTSGGYRRDSPKSATFTWKLLSSSMLLV 109
V+TWV S G R+ PKSAT TWK +S ML +
Sbjct: 104 AVETWVASSGKSRERPKSATLTWKSVSRRMLFL 6
Score = 70.3 bits (147), Expect(3) = 1e-021
Identities = 27/53 (50%), Positives = 37/53 (69%)
Frame = +1 / +3
Query: 106 EDQQHTAGQQLPREGRRLWAVAPVPS*SHPRVDRPTGHAGLRRPGVPPVLQAD 264
+++QH +LPR+GRRL + +P+ HPR+ RP GH +R PGVPPVL AD
Sbjct: 3 DEEQHPPRHRLPRQGRRLRPLPALPARRHPRLHRPPGHPRVRGPGVPPVLPAD 161
Score = 46.9 bits (96), Expect(3) = 1e-021
Identities = 20/37 (54%), Positives = 22/37 (59%)
Frame = +3 / +2
Query: 342 GATARSTWPTWLSTESRTTRWASWSTLSSGTRLTVRR 452
G RSTWP W ST SR+ R SW T SS + T RR
Sbjct: 239 GRGTRSTWPAWPSTRSRSARSRSWWTWSSASSRTRRR 349
Score = 31.8 bits (63), Expect(2) = 1e-037
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = -3 / -3
Query: 540 STTXXIDGRESLSSCKHRNASSAT 469
S+T I GR S C+HR ASSAT
Sbjct: 437 SSTSLIGGRISPFCCRHRKASSAT 366
Score = 23.1 bits (44), Expect(3) = 1e-021
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = +3 / +2
Query: 477 SWRSGACS*KGIHGRR*XKWW 539
SW SGACS RR + W
Sbjct: 374 SWPSGACSRTARCDRRSGRCW 436
>gb|AV836884.1|AV836884 AV836884 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd15l22, mRNA
sequence
Length = 687
Score = 199 bits (429), Expect = 4e-050
Identities = 83/106 (78%), Positives = 95/106 (89%)
Frame = +2 / -3
Query: 251 CYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELG 430
CYKLT+KSDVYSFGVVLVEL+SSKPAVDM RSHSEINLANMALNRIQNHEV QLVDPELG
Sbjct: 685 CYKLTDKSDVYSFGVVLVELISSKPAVDMNRSHSEINLANMALNRIQNHEVVQLVDPELG 506
Query: 431 YETDGETKKSIDLVAELAFRCLQLERDSRPSMXXVVEALNRIXNGD 568
Y+TD ETK++ID VAE+AF+CLQ+ER+ RPS+ VVE L + +GD
Sbjct: 505 YDTDPETKRTIDRVAEVAFQCLQMEREMRPSIKEVVEILTYVRDGD 368
Score = 87.7 bits (185), Expect(2) = 3e-024
Identities = 40/46 (86%), Positives = 42/46 (91%)
Frame = -3 / +3
Query: 387 ILLRAMLARLISLWLLPMSTAGLEDTSSTSTTPKLYTSLFSVSL*H 250
ILLRAMLARLISLWLL MSTAG E+ SST+TTPKLYTSL SVSL*H
Sbjct: 549 ILLRAMLARLISLWLLFMSTAGFEEISSTNTTPKLYTSLLSVSL*H 686
Score = 43.7 bits (89), Expect(2) = 3e-024
Identities = 19/29 (65%), Positives = 22/29 (75%)
Frame = -3 / +3
Query: 546 RASTTXXIDGRESLSSCKHRNASSATRSM 460
R STT IDGR SLS C+H A+SATRS+
Sbjct: 390 RISTTSLIDGRISLSICRHWKATSATRSI 476
>gb|AV929541.1|AV929541 AV929541 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd14j24 3', mRNA sequence
Length = 673
Score = 133 bits (285), Expect(2) = 1e-040
Identities = 54/80 (67%), Positives = 65/80 (81%)
Frame = +2 / -2
Query: 194 HVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANM 373
HVST PQGTPGYVDP Y Y+LT+KSDVYSFGVVL+ELVSS+PAVDM R +++NL +M
Sbjct: 630 HVSTAPQGTPGYVDPAYQHRYQLTDKSDVYSFGVVLMELVSSRPAVDMARPGTDVNLGSM 451
Query: 374 ALNRIQNHEVGQLVDPELGY 433
A+ IQ E+ +LVDP LGY
Sbjct: 450 AVRMIQCCEIDRLVDPRLGY 391
Score = 90.0 bits (190), Expect(2) = 4e-025
Identities = 43/64 (67%), Positives = 45/64 (70%)
Frame = -3 / +2
Query: 387 ILLRAMLARLISLWLLPMSTAGLEDTSSTSTTPKLYTSLFSVSL*HWWYTGST*PGVPCG 208
I+ AML R S+ MSTAGLEDTSS STTPKL TSL SVS WY GST PGVPCG
Sbjct: 437 IMRTAMLPRFTSVPGRAMSTAGLEDTSSMSTTPKL*TSLLSVSWYRCWYAGSTYPGVPCG 616
Query: 207 TVDT 196
VDT
Sbjct: 617 AVDT 628
Score = 54.2 bits (112), Expect(2) = 1e-040
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = +2 / -2
Query: 428 GYETDGETKKSIDLVAELAFRCLQLERDSRPSMXXVVEALNRIXNGDSLENNXT 589
G + ETK +IDLVAE+AFRCLQ E+D RPS+ V++ L + + E +
Sbjct: 390 GSSSASETKCTIDLVAEVAFRCLQPEQDVRPSIGEVLDVLRQAHQSVTAEKGAS 229
Score = 44.1 bits (90), Expect(2) = 4e-025
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = -3 / +2
Query: 540 STTXXIDGRESLSSCKHRNASSATRSM 460
S+T +DGR S S C+HRNA+SATRS+
Sbjct: 278 SSTSPMDGRTSCSGCRHRNATSATRSI 358
>gb|AV929540.1|AV929540 AV929540 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd14j23 3', mRNA sequence
Length = 656
Score = 126 bits (269), Expect(2) = 2e-038
Identities = 51/77 (66%), Positives = 62/77 (80%)
Frame = +2 / -2
Query: 203 TVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALN 382
T PQGTPGYVDP Y Y+LT+KSDVYSFGVVL+ELVSS+PAVDM R +++NL +MA+
Sbjct: 622 TAPQGTPGYVDPAYQHRYQLTDKSDVYSFGVVLMELVSSRPAVDMARPGTDVNLGSMAVR 443
Query: 383 RIQNHEVGQLVDPELGY 433
IQ E+ +LVDP LGY
Sbjct: 442 MIQCCEIDRLVDPRLGY 392
Score = 84.9 bits (179), Expect(2) = 1e-023
Identities = 41/62 (66%), Positives = 43/62 (69%)
Frame = -3 / +3
Query: 387 ILLRAMLARLISLWLLPMSTAGLEDTSSTSTTPKLYTSLFSVSL*HWWYTGST*PGVPCG 208
I+ AML R S+ MSTAGLEDTSS STTPKL TSL SVS WY GST PGVPCG
Sbjct: 438 IMRTAMLPRFTSVPGRAMSTAGLEDTSSMSTTPKL*TSLLSVSWYRCWYAGSTYPGVPCG 617
Query: 207 TV 202
V
Sbjct: 618 AV 623
Score = 54.2 bits (112), Expect(2) = 2e-038
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = +2 / -2
Query: 428 GYETDGETKKSIDLVAELAFRCLQLERDSRPSMXXVVEALNRIXNGDSLENNXT 589
G + ETK +IDLVAE+AFRCLQ E+D RPS+ V++ L + + E +
Sbjct: 391 GSSSASETKCTIDLVAEVAFRCLQPEQDVRPSIGEVLDVLRQAHQSVTAEKGAS 230
Score = 44.1 bits (90), Expect(2) = 1e-023
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = -3 / +3
Query: 540 STTXXIDGRESLSSCKHRNASSATRSM 460
S+T +DGR S S C+HRNA+SATRS+
Sbjct: 279 SSTSPMDGRTSCSGCRHRNATSATRSI 359
>gb|BU983256.1|BU983256 HA29B10r HA Hordeum vulgare subsp. vulgare cDNA clone HA29B10
5-PRIME, mRNA sequence
Length = 569
Score = 142 bits (304), Expect(2) = 1e-035
Identities = 59/113 (52%), Positives = 80/113 (70%)
Frame = +2 / +3
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
I HRD+K++NILLD KV+DFGLSRL P ++HVST QGT GY+DP Y++ Y+LT+
Sbjct: 42 IYHRDIKSSNILLDEQLDGKVSDFGLSRLAEPGLSHVSTCAQGTLGYLDPEYYRNYQLTD 221
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPEL 427
KSDVYSFGVVL+EL+++K A+D GR ++NLA + +VDP +
Sbjct: 222 KSDVYSFGVVLLELLTAKRAIDFGRGEDDVNLAVHVQRAADEERLLDVVDPAM 380
Score = 28.6 bits (56), Expect(2) = 1e-035
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2 / +3
Query: 479 LAFRCLQLERDSRPSMXXVVEALNRIXN 562
LA CL+ R +RPSM V + + I N
Sbjct: 432 LALGCLEDRRQNRPSMKEVADEIEYIIN 515
>gb|CD662706.1|CD662706 UCRHV18_02dd10_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_02dd10, mRNA sequence
Length = 851
Score = 133 bits (285), Expect(2) = 6e-035
Identities = 61/137 (44%), Positives = 89/137 (64%)
Frame = +2 / -1
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYK 259
A+ I HRDVK++NILLD+SF KV+DFG SR + THV T+ QGT GY+DP Y+ +
Sbjct: 728 AIPIFHRDVKSSNILLDSSFATKVSDFGASRSLSLDETHVVTIVQGTFGYLDPEYYHTGQ 549
Query: 260 LTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYET 439
LTEKSDVYSFGV+LVEL++ + + + + NL++ + +Q V +++DPE+ +
Sbjct: 548 LTEKSDVYSFGVILVELLTRRKPIFINDLGGKQNLSHYFVEGLQEGVVMEIMDPEVVKQA 369
Query: 440 DGETKKSIDLVAELAFR 490
+ E I +AE R
Sbjct: 368 NPEEIDDICSLAEACLR 318
Score = 35.0 bits (70), Expect(2) = 6e-035
Identities = 14/26 (53%), Positives = 18/26 (69%)
Frame = +2 / -1
Query: 17 LTWPRRMSIAIETAEALAYLHAVEII 94
L+W R+ IA+E A ALAYLH+ I
Sbjct: 800 LSWDDRIRIAVEAAGALAYLHSAAAI 723
>gb|CB879642.1|CB879642 HP03C03w HP Hordeum vulgare subsp. vulgare cDNA clone HP03C03
3-PRIME, mRNA sequence
Length = 544
Score = 88.6 bits (187), Expect(3) = 8e-030
Identities = 38/81 (46%), Positives = 57/81 (70%)
Frame = +2 / -2
Query: 185 EVTHVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINL 364
+ THVSTV +G+ GY+DP Y + +L+EKSDVYSFGVVL E++ ++PA+ +I+L
Sbjct: 351 DATHVSTVVKGSFGYLDPEYFRRQQLSEKSDVYSFGVVLFEVLCARPALSPSLPKEQISL 172
Query: 365 ANMALNRIQNHEVGQLVDPEL 427
A+ AL + +GQ++DP L
Sbjct: 171 ADWALRCQKQGVLGQIIDPML 109
Score = 51.0 bits (105), Expect(3) = 8e-030
Identities = 21/31 (67%), Positives = 25/31 (80%)
Frame = +2 / -2
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
IIHRDVKT NILLD+ + KV+DFGLS+ P
Sbjct: 450 IIHRDVKTTNILLDDKWVAKVSDFGLSKTGP 358
Score = 29.5 bits (58), Expect(3) = 8e-030
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +2 / -2
Query: 11 RGLTWPRRMSIAIETAEALAYLH 79
R L+W +R+ I I A L YLH
Sbjct: 537 RPLSWKKRLEICIGAARGLYYLH 469
>gb|BQ470048.1|BQ470048 HX01J23T HX Hordeum vulgare subsp. vulgare cDNA clone HX01J23
5-PRIME, mRNA sequence
Length = 567
Score = 117 bits (251), Expect(2) = 7e-029
Identities = 51/130 (39%), Positives = 81/130 (62%)
Frame = +2 / +2
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+++HRD+K++NILLD ++ KV+DFGL++L + +V+T GT GYV P Y L
Sbjct: 80 KVVHRDIKSSNILLDKRWNSKVSDFGLAKLLGSDSNYVTTRVMGTFGYVAPEYASTGMLN 259
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYETDG 445
E+SDVYSFGV+++E++S + VD R +E+NL N + ++DP+L +
Sbjct: 260 ERSDVYSFGVLIMEIISGRCPVDYARPPAEVNLVEWLKKMASNRDYEAVLDPKLPEKPTS 439
Query: 446 ETKKSIDLVA 475
+ K+ LVA
Sbjct: 440 KALKNALLVA 469
Score = 29.9 bits (59), Expect(2) = 7e-029
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L+W RM I + TA+ + YLH
Sbjct: 2 LSWDARMDIVLGTAKGITYLH 64
>gb|BM372335.2|BM372335 EBro03_SQ004_K20_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ004_K20 5', mRNA sequence
Length = 602
Score = 110 bits (234), Expect(2) = 4e-028
Identities = 44/81 (54%), Positives = 60/81 (74%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
I+HRDVKT NILLD + ++DFGLSR + TH+STV GT GY+DP YH ++LT
Sbjct: 253 IVHRDVKTPNILLDKNLVGVISDFGLSRAFNDAHTHISTVAAGTLGYLDPEYHATFQLTV 432
Query: 269 KSDVYSFGVVLVELVSSKPAV 331
K+DVYSFG+VL+E+++ + V
Sbjct: 433 KTDVYSFGIVLLEIITGQSPV 495
Score = 35.4 bits (71), Expect(2) = 4e-028
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +2 / +1
Query: 2 GSERGLTWPRRMSIAIETAEALAYLH 79
G + LTW +R+ IA++ A+ L YLH
Sbjct: 157 GDDYSLTWEQRLYIALDAAQGLEYLH 234
>gb|BF266777.2|BF266777 HV_CEa0016A13f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0016A13f, mRNA sequence
Length = 812
Score = 100 bits (212), Expect(3) = 4e-028
Identities = 44/78 (56%), Positives = 55/78 (70%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
I+H DVK++NILLD KVADFG S L P + + T+ GT GY+DP Y Q LT+
Sbjct: 163 ILHGDVKSSNILLDRDHKAKVADFGASILAPTDKSQFITLVHGTCGYLDPEYMQTNLLTD 342
Query: 269 KSDVYSFGVVLVELVSSK 322
KSDVYSFGVVL+EL++ K
Sbjct: 343 KSDVYSFGVVLLELLTGK 396
Score = 37.7 bits (76), Expect(3) = 4e-028
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = +2 / +1
Query: 446 ETKKSIDLVAELAFRCLQLERDSRPSMXXVVEALNRI 556
E+ + I+ AELA RCL++ D+RPS+ V E L+ +
Sbjct: 517 ESTEIIEEAAELAMRCLEMCGDNRPSIKEVAEKLDSL 627
Score = 28.6 bits (56), Expect(3) = 4e-028
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = +2 / +1
Query: 32 RMSIAIETAEALAYLHA 82
R+ IA E+A+ALAYLH+
Sbjct: 97 RLGIAHESADALAYLHS 147
>gb|BG414636.2|BG414636 HVSMEk0003C21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0003C21f, mRNA sequence
Length = 868
Score = 111 bits (236), Expect(2) = 7e-028
Identities = 49/130 (37%), Positives = 78/130 (60%)
Frame = +2 / +1
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+++HRD+K++NIL+D F+ K++DFGL+++ +H++T GT GYV P Y L
Sbjct: 307 KVVHRDIKSSNILIDEDFNGKLSDFGLAKMLGAGKSHITTRVMGTFGYVAPEYANTGLLN 486
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYETDG 445
EKSDVYSFGV+L+E V+ + VD GR +E++L + ++VD ++ +
Sbjct: 487 EKSDVYSFGVLLLEAVTGRDPVDYGRPANEVHLVEWLKMMVGTKRADEVVDRDI*VKPTI 666
Query: 446 ETKKSIDLVA 475
K LVA
Sbjct: 667 RASKRALLVA 696
Score = 33.6 bits (67), Expect(2) = 7e-028
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLH 79
LTW RM I + A+ALAYLH
Sbjct: 229 LTWEARMKIVLGIAKALAYLH 291
>gb|BI952328.1|BI952328 HVSMEm0005P11f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0005P11f, mRNA sequence
Length = 1207
Score = 124 bits (266), Expect = 7e-028
Identities = 63/142 (44%), Positives = 82/142 (57%)
Frame = +2 / +1
Query: 83 VEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKL 262
V I HRDVK+ NILLD ++ KV+DFG SRL P + THV T QGT GY+DP Y+ L
Sbjct: 73 VSIFHRDVKSTNILLDGNYTAKVSDFGASRLVPIDQTHVVTNIQGTFGYLDPEYYHTGML 252
Query: 263 TEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYETD 442
EKSDVYSFGVVLVEL+ K + S NL+N L ++ + ++V ++ E
Sbjct: 253 NEKSDVYSFGVVLVELLLPKKPIFTSDSGLTQNLSNYFLWEMREKPLAEIVATQVLEEAT 432
Query: 443 GETKKSIDLVAELAFRCLQLER 508
E I +AE + ER
Sbjct: 433 NEEINDIATLAETCLQLRGEER 498
>gb|AV917322.1|AV917322 AV917322 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18g23 5', mRNA sequence
Length = 578
Score = 112 bits (239), Expect(2) = 1e-027
Identities = 49/97 (50%), Positives = 68/97 (70%)
Frame = +2 / +3
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+IIHRD+K++NILLD F KVADFGL++ THVST GT GY+ P Y KLT
Sbjct: 81 KIIHRDIKSSNILLDFKFEAKVADFGLAKFTSDNNTHVSTRVMGTFGYLAPEYASSGKLT 260
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMA 376
EKSDV+SFGV+L+EL++ + VD +++ + +L + A
Sbjct: 261 EKSDVFSFGVMLLELITGRRPVDSTQTYMDDSLVDWA 371
Score = 31.3 bits (62), Expect(2) = 1e-027
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L WP R+ IA+ A+ LAY+H
Sbjct: 3 LEWPIRLRIALGAAKGLAYIH 65
>gb|AV916932.1|AV916932 AV916932 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20c08 5', mRNA sequence
Length = 446
Score = 112 bits (239), Expect(2) = 1e-027
Identities = 49/97 (50%), Positives = 68/97 (70%)
Frame = +2 / +2
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+IIHRD+K++NILLD F KVADFGL++ THVST GT GY+ P Y KLT
Sbjct: 134 KIIHRDIKSSNILLDFKFEAKVADFGLAKFTSDNNTHVSTRVMGTFGYLAPEYASSGKLT 313
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMA 376
EKSDV+SFGV+L+EL++ + VD +++ + +L + A
Sbjct: 314 EKSDVFSFGVMLLELITGRRPVDSTQTYMDDSLVDWA 424
Score = 31.3 bits (62), Expect(2) = 1e-027
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L WP R+ IA+ A+ LAY+H
Sbjct: 56 LEWPIRLRIALGAAKGLAYIH 118
>gb|AW982539.2|AW982539 HVSMEg0003I16f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003I16f, mRNA sequence
Length = 933
Score = 110 bits (234), Expect(2) = 2e-027
Identities = 49/97 (50%), Positives = 66/97 (68%)
Frame = +2 / +3
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+IIHRD+K +NILLD KVADFGL++L THVST GT GY+ P Y KLT
Sbjct: 270 KIIHRDIKASNILLDYRCEAKVADFGLAKLTSDNNTHVSTRVMGTFGYLAPEYASSGKLT 449
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMA 376
EKSDV+SFGV+L+EL++ + V ++H + +L + A
Sbjct: 450 EKSDVFSFGVMLLELITERRPVSSKQAHMDDSLXDWA 560
Score = 32.7 bits (65), Expect(2) = 2e-027
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
+ WP R+ IA+ +A+ LAYLH
Sbjct: 192 MDWPSRLRIALGSAKGLAYLH 254
>gb|BI780740.2|BI780740 EBes01_SQ002_A07_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ002_A07 5', mRNA sequence
Length = 601
Score = 91.8 bits (194), Expect(2) = 8e-027
Identities = 38/79 (48%), Positives = 55/79 (69%)
Frame = +2 / +2
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST +G+ GY+DP Y++ KLT+KSDVYSFGVVL+E++ ++P +D INLA
Sbjct: 134 THVSTAVKGSFGYLDPEYYRRQKLTDKSDVYSFGVVLLEVICARPVIDPTLPRDMINLAE 313
Query: 371 MALNRIQNHEVGQLVDPEL 427
A+ + E+GQ+VD +
Sbjct: 314 WAIKWQKRGELGQIVDQRI 370
Score = 49.2 bits (101), Expect(2) = 8e-027
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = +2 / +2
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
A IIHRDVK+ NILLD + KV+DFGLS+ P
Sbjct: 20 AKSIIHRDVKSANILLDENLMAKVSDFGLSKTGP 121
>gb|BU967283.1|BU967283 HB03N04r BC Hordeum vulgare subsp. vulgare cDNA clone HB03N04
5-PRIME, mRNA sequence
Length = 585
Score = 91.8 bits (194), Expect(2) = 8e-027
Identities = 38/79 (48%), Positives = 55/79 (69%)
Frame = +2 / +2
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST +G+ GY+DP Y++ KLT+KSDVYSFGVVL+E++ ++P +D INLA
Sbjct: 161 THVSTAVKGSFGYLDPEYYRRQKLTDKSDVYSFGVVLLEVICARPVIDPTLPRDMINLAE 340
Query: 371 MALNRIQNHEVGQLVDPEL 427
A+ + E+GQ+VD +
Sbjct: 341 WAIKWQKRGELGQIVDQRI 397
Score = 49.2 bits (101), Expect(2) = 8e-027
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = +2 / +2
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
A IIHRDVK+ NILLD + KV+DFGLS+ P
Sbjct: 47 AKSIIHRDVKSANILLDENLMAKVSDFGLSKTGP 148
>gb|BU966755.1|BU966755 HB02E05r BC Hordeum vulgare subsp. vulgare cDNA clone HB02E05
5-PRIME, mRNA sequence
Length = 531
Score = 91.8 bits (194), Expect(2) = 8e-027
Identities = 38/79 (48%), Positives = 55/79 (69%)
Frame = +2 / +1
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST +G+ GY+DP Y++ KLT+KSDVYSFGVVL+E++ ++P +D INLA
Sbjct: 160 THVSTAVKGSFGYLDPEYYRRQKLTDKSDVYSFGVVLLEVICARPVIDPTLPRDMINLAE 339
Query: 371 MALNRIQNHEVGQLVDPEL 427
A+ + E+GQ+VD +
Sbjct: 340 WAIKWQKRGELGQIVDQRI 396
Score = 49.2 bits (101), Expect(2) = 8e-027
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = +2 / +1
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
A IIHRDVK+ NILLD + KV+DFGLS+ P
Sbjct: 46 AKSIIHRDVKSANILLDENLMAKVSDFGLSKTGP 147
>gb|BF266815.2|BF266815 HV_CEa0016C07f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0016C07f, mRNA sequence
Length = 847
Score = 93.2 bits (197), Expect(4) = 1e-026
Identities = 42/136 (30%), Positives = 74/136 (54%)
Frame = +2 / +3
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST +G+ GY+DP Y + +LT++SDVYSFGVVL+E++ ++P +D +IN+A
Sbjct: 267 THVSTAVKGSFGYLDPGYFKTQQLTDRSDVYSFGVVLLEVLCARPVIDQSLERGQINIAE 446
Query: 371 MALNRIQNHEVGQLVDPELGYETDGETKKSIDLVAELAFRCLQLERDSRPSMXXVVEALN 550
A+ + ++ DP + E D E+ + A+ ++R S + +E
Sbjct: 447 WAVRMRGEGGLDKIADPRIAGEADDESLRKFAETADKCLADCWVDRPSMGDVLWNLEFWL 626
Query: 551 RIXNGDSLENNXTDRS 598
++ D ++ +D S
Sbjct: 627 QLHEADVTGDDASDNS 674
Score = 29.9 bits (59), Expect(4) = 1e-026
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +2 / +3
Query: 77 HAVEIIHRDVKTNNILL 127
+A IIHRDVK+ NILL
Sbjct: 126 YAENIIHRDVKSTNILL 176
Score = 27.2 bits (53), Expect(4) = 1e-026
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L+W +R+ I I A L YLH
Sbjct: 57 LSWRQRLEICIGAARGLHYLH 119
Score = 25.4 bits (49), Expect(4) = 1e-026
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +2 / +3
Query: 146 KVADFGLSRLYP 181
KVADFGLSR+ P
Sbjct: 219 KVADFGLSRVGP 254
>gb|BF261904.2|BF261904 HV_CEa0002I16f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0002I16f, mRNA sequence
Length = 849
Score = 109 bits (233), Expect(2) = 1e-026
Identities = 53/156 (33%), Positives = 89/156 (57%)
Frame = +2 / +3
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYK 259
+V I+HRD+K+ N+LL++S+ KV+DFG SRL + TH+ T QGT GY+DP Y+ +
Sbjct: 255 SVLILHRDIKSMNVLLNDSYTDKVSDFGASRLIRIDQTHLGTAVQGTYGYLDPEYYYTAQ 434
Query: 260 LTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYET 439
L EKSDV++FGV+ L++ + + + NLAN ++ + + VD ++ +
Sbjct: 435 LNEKSDVHTFGVIPGVLLTRRTPIIQNEHGEKQNLANYFSRAMRERPLKETVDAQIQRDG 614
Query: 440 DGETKKSIDLVAELAFRCLQLERDSRPSMXXVVEAL 547
E S+ VAE + E+ + M +++ L
Sbjct: 615 PDEGGLSMAPVAEECLNLTREEKPTMNDMQIMLQLL 722
Score = 30.9 bits (61), Expect(2) = 1e-026
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLHA 82
L+W R+ I IE A AL YLH+
Sbjct: 183 LSWEERLRIYIEIAGALTYLHS 248
>gb|BJ450289.1|BJ450289 BJ450289 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak28p18 5', mRNA sequence
Length = 609
Score = 112 bits (240), Expect(2) = 1e-026
Identities = 55/165 (33%), Positives = 96/165 (58%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
I+H DVK++NILLD++ KV+DFG S L P + + T+ QGT GY+DP Y Q +LT+
Sbjct: 91 ILHGDVKSSNILLDDNHKAKVSDFGASILAPTDESQFVTLVQGTCGYLDPEYMQTCQLTD 270
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYETDGE 448
KSDVYSFGVVL+EL++ K ++ + +L+ M ++ ++ + + ++D + E + E
Sbjct: 271 KSDVYSFGVVLLELLTCKKPFNLDAIGQDKSLSMMFMSAMKENRLEVILDDGIKDEDNME 450
Query: 449 TKKSIDLVAELAFRCLQLERDSRPSMXXVVEALNRIXNGDSLENN 583
+ I +A+ R S + ++ L ++ + ++ N
Sbjct: 451 ILEEIAELAKHCLEMSGENRPSMKEVAEKLDRLRKVMHHPWVQRN 585
Score = 27.2 bits (53), Expect(2) = 1e-026
Identities = 10/17 (58%), Positives = 15/17 (88%)
Frame = +2 / +1
Query: 32 RMSIAIETAEALAYLHA 82
R+ IA ++AEAL+YLH+
Sbjct: 25 RLGIAHDSAEALSYLHS 75
>gb|AV833754.1|AV833754 AV833754 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags17h16, mRNA sequence
Length = 549
Score = 113 bits (241), Expect(2) = 1e-026
Identities = 52/111 (46%), Positives = 72/111 (64%)
Frame = +2 / +2
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
IIHRD++ NNIL+ + F V DFGL+R P V T GT GY+ P Y Q ++TE
Sbjct: 209 IIHRDMRPNNILVTHDFEPLVGDFGLARWQPDGDMGVETRVIGTFGYLAPEYAQSGQITE 388
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDP 421
K+DVYSFGVVLVELV+ + AVD+ R + L A + ++++ V +L+DP
Sbjct: 389 KADVYSFGVVLVELVTGRKAVDINRPKGQQFLTEWARHLLEDNAVDELIDP 541
Score = 26.7 bits (52), Expect(2) = 1e-026
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L W R IA+ A L YLH
Sbjct: 125 LEWAARQKIAVGAARGLRYLH 187
>gb|CK123771.1|CK123771 BES1824105f02 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010F025 5-PRIME, mRNA sequence
Length = 808
Score = 72.1 bits (151), Expect(3) = 8e-026
Identities = 36/69 (52%), Positives = 43/69 (62%)
Frame = +2 / +1
Query: 194 HVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANM 373
HVST GT GYV P Y LT KSDVYS+GVVL+EL++ + VDM RS E L N
Sbjct: 334 HVSTRVLGTQGYVAPEYALTGHLTTKSDVYSYGVVLLELLTGRVPVDMKRSPGEGVLVNW 513
Query: 374 ALNRIQNHE 400
AL + + E
Sbjct: 514 ALPMLTDRE 540
Score = 51.5 bits (106), Expect(3) = 8e-026
Identities = 18/29 (62%), Positives = 25/29 (86%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRL 175
+IHRD K++NILLD FH +V+DFGL++L
Sbjct: 226 VIHRDFKSSNILLDKDFHARVSDFGLAKL 312
Score = 31.8 bits (63), Expect(3) = 8e-026
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L W RM IA+E A+ L YLH
Sbjct: 145 LDWSTRMRIALEAAKGLEYLH 207
>gb|BI952975.1|BI952975 HVSMEm0008M21f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0008M21f, mRNA sequence
Length = 1290
Score = 101 bits (216), Expect(2) = 9e-026
Identities = 45/95 (47%), Positives = 65/95 (68%)
Frame = +2 / +2
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYK 259
A+ I HRDVK++NILLD+SF KV+DFG SR + THV T+ Q T GY++P Y+ +
Sbjct: 221 AIPIFHRDVKSSNILLDSSFATKVSDFGASRSLSLDETHVVTIVQRTFGYLNPEYYHTGQ 400
Query: 260 LTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINL 364
LTEKSDV + G++LVEL++ + + ++NL
Sbjct: 401 LTEKSDVSNSGIILVELLTRTTPMFIHDLRGKLNL 505
Score = 35.4 bits (71), Expect(2) = 9e-026
Identities = 14/26 (53%), Positives = 18/26 (69%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLHAVEII 94
L+W R+ IA+E A ALAYLH+ I
Sbjct: 149 LSWDDRIXIAVEAAGALAYLHSAAAI 226
>gb|AW983189.3|AW983189 HVSMEg0008J16f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0008J16f, mRNA sequence
Length = 877
Score = 87.7 bits (185), Expect(2) = 1e-025
Identities = 42/127 (33%), Positives = 70/127 (55%)
Frame = +2 / +3
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST +G+ GY+DP Y + +LTEKSDVYSFGVV++E++ ++P +D +NLA
Sbjct: 126 THVSTAVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVMLEVLCARPVIDPSLPREMVNLAE 305
Query: 371 MALNRIQNHEVGQLVDPELGYETDGETKKSIDLVAELAFRCLQLERDSRPSMXXVVEALN 550
+ + E+ Q+VD +L ++ + E +ER S + +E +
Sbjct: 306 WGMKWQKRGELHQIVDQKLSGAIRPDSLRKFGETVEKCLADYGVERPSMGDVLWNLEYVL 485
Query: 551 RIXNGDS 571
++ + DS
Sbjct: 486 QLQDADS 506
Score = 49.6 bits (102), Expect(2) = 1e-025
Identities = 22/34 (64%), Positives = 25/34 (73%)
Frame = +2 / +3
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
A IIHRDVK+ NILLD + KVADFGLS+ P
Sbjct: 12 AKAIIHRDVKSANILLDENLLAKVADFGLSKTGP 113
>gb|CB878070.1|CB878070 HP07D18T HP Hordeum vulgare subsp. vulgare cDNA clone HP07D18
5-PRIME, mRNA sequence
Length = 355
Score = 117 bits (250), Expect = 1e-025
Identities = 55/100 (55%), Positives = 66/100 (66%)
Frame = +2 / +1
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYK 259
+V I HRDVK+ NILLD ++ KV+DFG SRL P + THV T QGT GY+DP Y+
Sbjct: 37 SVSIFHRDVKSTNILLDGNYTAKVSDFGASRLVPIDQTHVVTNIQGTFGYLDPEYYHTGM 216
Query: 260 LTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMAL 379
L EKSDVYSFGVVLVEL+ K + S NL+N L
Sbjct: 217 LNEKSDVYSFGVVLVELLLRKKPIFTSDSGLTQNLSNYFL 336
>gb|BE060160.1|BE060160 HVSMEg0011B12f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011B12f, mRNA sequence
Length = 553
Score = 102 bits (218), Expect(2) = 1e-025
Identities = 41/85 (48%), Positives = 60/85 (70%)
Frame = +2 / +2
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+++HRD+K++NIL+D+ F KV+DFGL++L +HV+T GT GYV P Y L
Sbjct: 278 KVVHRDIKSSNILIDDDFDAKVSDFGLAKLLGAGKSHVTTRVMGTFGYVAPEYANTGLLN 457
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMG 340
EKSD+YSFGVV++E ++ + VD G
Sbjct: 458 EKSDIYSFGVVILEAITGEDPVDYG 532
Score = 34.1 bits (68), Expect(2) = 1e-025
Identities = 13/21 (61%), Positives = 16/21 (76%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLH 79
LTW R+ I + TA+ALAYLH
Sbjct: 200 LTWEARIKILLGTAKALAYLH 262
>gb|BG367446.2|BG367446 HVSMEi0012E18f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0012E18f, mRNA sequence
Length = 689
Score = 116 bits (249), Expect = 2e-025
Identities = 48/114 (42%), Positives = 73/114 (64%)
Frame = +2 / +2
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+++HRD+K++NIL+D+ F KV+DFGL++L +HV+T GT GYV P Y L
Sbjct: 29 KVVHRDIKSSNILIDDDFDAKVSDFGLAKLLGAGKSHVTTRVMGTFGYVAPEYANTGLLN 208
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPEL 427
EKSD+YSFGVV++E ++ + VD GR E+NL + + ++VDP +
Sbjct: 209 EKSDIYSFGVVILEAITGRDPVDYGRPTKEVNLVDWLKMMVAIRRSEEVVDPTI 370
>gb|BU986512.1|BU986512 HF12B10r HF Hordeum vulgare subsp. vulgare cDNA clone HF12B10
5-PRIME, mRNA sequence
Length = 651
Score = 75.8 bits (159), Expect(3) = 2e-025
Identities = 29/57 (50%), Positives = 44/57 (77%)
Frame = +2 / +2
Query: 185 EVTHVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSE 355
E TH+ST +GT GY+DP Y + Y+LT KSDV+SFG++LVE++S++ V++ R+ E
Sbjct: 206 EKTHISTKVKGTAGYLDPEYLRTYQLTPKSDVFSFGILLVEIISARRPVELKRATEE 376
Score = 48.7 bits (100), Expect(3) = 2e-025
Identities = 19/33 (57%), Positives = 26/33 (78%)
Frame = +2 / +2
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPE 187
IIHRDVK++NILL +S+ KV+DFG +R P +
Sbjct: 104 IIHRDVKSSNILLTDSYRAKVSDFGFARSGPSD 202
Score = 29.5 bits (58), Expect(3) = 2e-025
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +2 / +2
Query: 11 RGLTWPRRMSIAIETAEALAYLH 79
R L + +R+ IAI+ A AL YLH
Sbjct: 17 RTLDFNQRLEIAIDVAHALTYLH 85
>gb|BJ464341.1|BJ464341 BJ464341 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags33o06 5', mRNA sequence
Length = 650
Score = 77.1 bits (162), Expect(3) = 2e-025
Identities = 32/73 (43%), Positives = 48/73 (65%)
Frame = +2 / +3
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST +G+ GY+DP Y + +LTEKSDVYSFGVV++E++ ++P +D +NLA
Sbjct: 432 THVSTAVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVMLEVLCARPVIDPSLPREMVNLAE 611
Query: 371 MALNRIQNHEVGQ 409
+ + E+ Q
Sbjct: 612 WGMKWQKRGELHQ 650
Score = 49.6 bits (102), Expect(3) = 2e-025
Identities = 22/34 (64%), Positives = 25/34 (73%)
Frame = +2 / +3
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
A IIHRDVK+ NILLD + KVADFGLS+ P
Sbjct: 318 AKAIIHRDVKSANILLDENLLAKVADFGLSKTGP 419
Score = 27.2 bits (53), Expect(3) = 2e-025
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L W +R+ I I A L YLH
Sbjct: 246 LNWKQRLEICIGAARGLHYLH 308
>gb|BQ768749.1|BQ768749 EBro08_SQ012_G15_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ012_G15 5', mRNA sequence
Length = 635
Score = 67.0 bits (140), Expect(3) = 3e-025
Identities = 24/60 (40%), Positives = 42/60 (70%)
Frame = +2 / +1
Query: 2 GSERGLTWPRRMSIAIETAEALAYLHAVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
GS+ L W R+++A++ A +A+LH+ ++I RD+K++N+LL K++DFGL+R P
Sbjct: 91 GSQPPLPWSTRVAVAVDVARGIAFLHSRDVIFRDLKSSNVLLGPDHRAKLSDFGLARAGP 270
Score = 59.7 bits (124), Expect(3) = 3e-025
Identities = 29/67 (43%), Positives = 40/67 (59%)
Frame = +2 / +1
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
+HVST GT GY P Y L+ KSDVY FGVVL+EL++ + A+D R + L +
Sbjct: 283 SHVSTRVVGTRGYAAPEYVATGHLSAKSDVYGFGVVLLELMTGRRALDESRGLASELLVD 462
Query: 371 MALNRIQ 391
A+ +Q
Sbjct: 463 WAMPMLQ 483
Score = 26.7 bits (52), Expect(3) = 3e-025
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2 / +1
Query: 452 KKSIDLVAELAFRCLQLERDSRPSM 526
K+ +A LA RCLQ + SRP M
Sbjct: 541 KRQAQDMAALALRCLQNDPKSRPFM 615
>gb|AV913572.1|AV913572 AV913572 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags22i21 5', mRNA sequence
Length = 634
Score = 107 bits (229), Expect(2) = 3e-025
Identities = 43/92 (46%), Positives = 65/92 (70%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
IIHRD+K++NILLD +F +V+DFGL++L E +H++T+ GT GY+ P Y Q + TE
Sbjct: 325 IIHRDIKSSNILLDGNFESRVSDFGLAKLLEDEESHITTIVAGTFGYLAPEYMQSGRATE 504
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINL 364
K+DVYSFGV+++E++S K D +N+
Sbjct: 505 KTDVYSFGVLVLEILSGKRPTDASFIEKGLNI 600
Score = 27.6 bits (54), Expect(2) = 3e-025
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L W R++I + A+ LAYLH
Sbjct: 244 LEWEARINIILGAAKGLAYLH 306
>gb|CA032771.1|CA032771 HX14C15r HX Hordeum vulgare subsp. vulgare cDNA clone HX14C15
5-PRIME, mRNA sequence
Length = 638
Score = 105 bits (224), Expect(2) = 6e-025
Identities = 38/78 (48%), Positives = 60/78 (76%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
I+HRD+K +NIL+D ++ K+ DFGL++L+P +TH+ST GT GY+ P Y +LT+
Sbjct: 229 IVHRDIKASNILMDKNYVPKIGDFGLAKLFPDNITHISTRVAGTTGYLAPEYAWHGQLTK 408
Query: 269 KSDVYSFGVVLVELVSSK 322
K+D+YSFGV+++E++S K
Sbjct: 409 KADIYSFGVLVIEVISGK 462
Score = 29.0 bits (57), Expect(2) = 6e-025
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = +2 / +1
Query: 20 TWPRRMSIAIETAEALAYLH 79
TW R SI I A LAYLH
Sbjct: 151 TWSVRSSICIGVARGLAYLH 210
>gb|BU972150.1|BU972150 HB20M05r BC Hordeum vulgare subsp. vulgare cDNA clone HB20M05
5-PRIME, mRNA sequence
Length = 617
Score = 105 bits (223), Expect(2) = 8e-025
Identities = 43/93 (46%), Positives = 65/93 (69%)
Frame = +2 / +3
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+++HRDVK++NILLD ++ K++DFGL++L E ++V+T GT GYV P Y L
Sbjct: 201 KVVHRDVKSSNILLDKHWNAKLSDFGLAKLLGSERSYVTTRVMGTFGYVAPEYAGTGMLN 380
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINL 364
E SDVYSFG++++E++S + VD R E+NL
Sbjct: 381 ETSDVYSFGILIMEIISGRVPVDYNRPPGEVNL 479
Score = 29.0 bits (57), Expect(2) = 8e-025
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
LTW RM I + +A+ L YLH
Sbjct: 123 LTWDIRMKIILGSAKGLMYLH 185
>gb|AV916201.1|AV916201 AV916201 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags12b05 5', mRNA sequence
Length = 593
Score = 84.5 bits (178), Expect(2) = 8e-025
Identities = 35/79 (44%), Positives = 53/79 (67%)
Frame = +2 / +2
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST +G+ GY+DP Y + +LTEKSDVYSFGVV++E++ ++P +D +NLA
Sbjct: 317 THVSTAVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVMLEVLCARPVIDPSLPREMVNLAE 496
Query: 371 MALNRIQNHEVGQLVDPEL 427
+ + E+ Q+VD +L
Sbjct: 497 WGMKWQKRGELHQIVDQKL 553
Score = 49.6 bits (102), Expect(2) = 8e-025
Identities = 22/34 (64%), Positives = 25/34 (73%)
Frame = +2 / +2
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
A IIHRDVK+ NILLD + KVADFGLS+ P
Sbjct: 203 AKAIIHRDVKSANILLDENLLAKVADFGLSKTGP 304
>gb|BU971791.1|BU971791 HB19L01r BC Hordeum vulgare subsp. vulgare cDNA clone HB19L01
5-PRIME, mRNA sequence
Length = 510
Score = 105 bits (223), Expect(2) = 8e-025
Identities = 43/93 (46%), Positives = 65/93 (69%)
Frame = +2 / +2
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+++HRDVK++NILLD ++ K++DFGL++L E ++V+T GT GYV P Y L
Sbjct: 200 KVVHRDVKSSNILLDKHWNAKLSDFGLAKLLGSERSYVTTRVMGTFGYVAPEYAGTGMLN 379
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINL 364
E SDVYSFG++++E++S + VD R E+NL
Sbjct: 380 ETSDVYSFGILIMEIISGRVPVDYNRPPGEVNL 478
Score = 29.0 bits (57), Expect(2) = 8e-025
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLH 79
LTW RM I + +A+ L YLH
Sbjct: 122 LTWDIRMKIILGSAKGLMYLH 184
>gb|CA021794.1|CA021794 HZ41C07r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ41C07
5-PRIME, mRNA sequence
Length = 452
Score = 101 bits (215), Expect(2) = 8e-025
Identities = 46/100 (46%), Positives = 63/100 (63%)
Frame = +2 / +3
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
IIHRDVK+ NILLD +ADFG+++ TH ST GT GY+DP Y + +L E
Sbjct: 108 IIHRDVKSKNILLDKDNVAHLADFGIAKSVCISKTHTSTYVMGTIGYIDPEYARTSRLNE 287
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRI 388
KSDVYS+G+VL+EL++ K VD + + L+ A N +
Sbjct: 288 KSDVYSYGIVLLELLTGKKPVDNECNLHHLILSKAADNTV 407
Score = 32.7 bits (65), Expect(2) = 8e-025
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L W R+ IA+ TA+ LAYLH
Sbjct: 27 LDWEARLQIALGTAQGLAYLH 89
>gb|BI777999.2|BI777999 EBro07_SQ001_M06_R root, 3 week, reduced light, cv Optic, EBro07
Hordeum vulgare subsp. vulgare cDNA clone
EBro07_SQ001_M06 5', mRNA sequence
Length = 450
Score = 100 bits (212), Expect(2) = 1e-024
Identities = 40/85 (47%), Positives = 60/85 (70%)
Frame = +2 / +1
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+++HRD+K++NIL+D F+ K++DFGL+++ +H++T GT GYV P Y L
Sbjct: 196 KVVHRDIKSSNILIDEDFNGKLSDFGLAKMLGAGKSHITTRVMGTFGYVAPEYANTGLLN 375
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMG 340
EKSDVYSFGV+L+E V+ + VD G
Sbjct: 376 EKSDVYSFGVLLLEAVTGRDPVDYG 450
Score = 33.6 bits (67), Expect(2) = 1e-024
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLH 79
LTW RM I + A+ALAYLH
Sbjct: 118 LTWEARMKIVLGIAKALAYLH 180
>gb|AV833368.1|AV833368 AV833368 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags7e10, mRNA sequence
Length = 523
Score = 71.6 bits (150), Expect(3) = 1e-024
Identities = 28/49 (57%), Positives = 39/49 (79%)
Frame = +2 / +2
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDM 337
T +S +P GT GY+DP Y++ + LT KSDVYSFGVVL+E++S + A+DM
Sbjct: 224 TPLSELPAGTLGYLDPEYYRLHYLTTKSDVYSFGVVLLEILSGRKAIDM 370
Score = 47.3 bits (97), Expect(3) = 1e-024
Identities = 17/31 (54%), Positives = 25/31 (80%)
Frame = +2 / +2
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
+IHRD+K++NIL+D + +VADFGLS + P
Sbjct: 119 VIHRDIKSSNILIDEDHNARVADFGLSIMGP 211
Score = 32.2 bits (64), Expect(3) = 1e-024
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +2 / +2
Query: 8 ERGLTWPRRMSIAIETAEALAYLH 79
++ L W RR++IA++ A + YLH
Sbjct: 29 KKQLNWTRRVTIAVQAARGIEYLH 100
>gb|AV932936.1|AV932936 AV932936 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal4l17 5', mRNA sequence
Length = 681
Score = 88.6 bits (187), Expect(2) = 3e-024
Identities = 38/81 (46%), Positives = 57/81 (70%)
Frame = +2 / +1
Query: 185 EVTHVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINL 364
+ THVSTV +G+ GY+DP Y + +L+EKSDVYSFGVVL E++ ++PA+ +I+L
Sbjct: 91 DATHVSTVVKGSFGYLDPEYFRRQQLSEKSDVYSFGVVLFEVLCARPALSPSLPKEQISL 270
Query: 365 ANMALNRIQNHEVGQLVDPEL 427
A+ AL + +GQ++DP L
Sbjct: 271 ADWALRCQKQGVLGQIIDPML 333
Score = 43.7 bits (89), Expect(2) = 3e-024
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +2 / +1
Query: 98 RDVKTNNILLDNSFHVKVADFGLSRLYP 181
RDVKT NILLD+ + KV+DFGLS+ P
Sbjct: 1 RDVKTTNILLDDKWVAKVSDFGLSKTGP 84
>gb|BQ760957.1|BQ760957 EBro03_SQ003_P10_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ003_P10 5', mRNA sequence
Length = 592
Score = 100 bits (213), Expect(2) = 3e-024
Identities = 39/81 (48%), Positives = 59/81 (72%)
Frame = +2 / +1
Query: 83 VEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKL 262
+ ++HRD+K +NILLD + + K++DFGL++LY + THVST GT GY+ P Y +
Sbjct: 280 IPVVHRDIKASNILLDANLNPKISDFGLAKLYDDQKTHVSTKVAGTFGYLAPEYAMRGHM 459
Query: 263 TEKSDVYSFGVVLVELVSSKP 325
TEK DV++FGVV++E +S +P
Sbjct: 460 TEKIDVFAFGVVVLETLSGRP 522
Score = 31.8 bits (63), Expect(2) = 3e-024
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLHAVEII 94
L WP R I + A L+YLH II
Sbjct: 205 LYWPTRFEICLSVARGLSYLHEESII 282
>gb|BU971391.1|BU971391 HB17H15r BC Hordeum vulgare subsp. vulgare cDNA clone HB17H15
5-PRIME, mRNA sequence
Length = 552
Score = 107 bits (229), Expect(2) = 3e-024
Identities = 49/127 (38%), Positives = 75/127 (59%)
Frame = +2 / +3
Query: 95 HRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTEKS 274
HRD+K++NIL+D F+ K++DFGL+++ +H++T GT GYV P Y L EKS
Sbjct: 12 HRDIKSSNILIDEDFNGKLSDFGLAKMLGAGKSHITTRVMGTFGYVAPEYANTGLLNEKS 191
Query: 275 DVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYETDGETK 454
DVYSFGV+L+E V+ + VD GR +E++L + ++VD ++ +
Sbjct: 192 DVYSFGVLLLEAVTGRDPVDYGRPANEVHLVEWLKMMVGTKRADEVVDRDMEVKPTIRAL 371
Query: 455 KSIDLVA 475
K LVA
Sbjct: 372 KRALLVA 392
Score = 24.4 bits (47), Expect(2) = 3e-024
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +2 / +3
Query: 479 LAFRCLQLERDSRPSMXXVVEAL 547
+A RC+ + + RP+M VV L
Sbjct: 387 VALRCVDPDSEKRPTMGHVVRML 455
>gb|BJ471888.1|BJ471888 BJ471888 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal28e24 5', mRNA sequence
Length = 520
Score = 88.6 bits (187), Expect(2) = 3e-024
Identities = 38/81 (46%), Positives = 57/81 (70%)
Frame = +2 / +1
Query: 185 EVTHVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINL 364
+ THVSTV +G+ GY+DP Y + +L+EKSDVYSFGVVL E++ ++PA+ +I+L
Sbjct: 91 DATHVSTVVKGSFGYLDPEYFRRQQLSEKSDVYSFGVVLFEVLCARPALSPSLPKEQISL 270
Query: 365 ANMALNRIQNHEVGQLVDPEL 427
A+ AL + +GQ++DP L
Sbjct: 271 ADWALRCQKQGVLGQIIDPML 333
Score = 43.7 bits (89), Expect(2) = 3e-024
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +2 / +1
Query: 98 RDVKTNNILLDNSFHVKVADFGLSRLYP 181
RDVKT NILLD+ + KV+DFGLS+ P
Sbjct: 1 RDVKTTNILLDDKWVAKVSDFGLSKTGP 84
>gb|BQ764108.1|BQ764108 EBro03_SQ006_E12_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ006_E12 5', mRNA sequence
Length = 632
Score = 82.6 bits (174), Expect(4) = 3e-024
Identities = 34/96 (35%), Positives = 57/96 (59%)
Frame = +2 / +2
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST +G+ GY+DP Y + +LT++SDVYSFGVVL E++ ++P +D +IN+A
Sbjct: 305 THVSTAVKGSFGYLDPEYFKTQQLTDRSDVYSFGVVLFEVLCARPVIDQSLDRDQINIAE 484
Query: 371 MALNRIQNHEVGQLVDPELGYETDGETKKSIDLVAE 478
A+ ++ ++ D + E + + + AE
Sbjct: 485 WAVRMHGEGKLDKIADARIAGEVNENSLRKFAETAE 592
Score = 30.4 bits (60), Expect(4) = 3e-024
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / +2
Query: 77 HAVEIIHRDVKTNNILLDNSFH 142
++ IIHRDVK+ NILL H
Sbjct: 152 YSENIIHRDVKSTNILLGTDDH 217
Score = 28.1 bits (55), Expect(4) = 3e-024
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2 / +2
Query: 8 ERGLTWPRRMSIAIETAEALAYLH 79
E L+W +R+ I I A L YLH
Sbjct: 74 EPTLSWKQRLEICIGAARGLHYLH 145
Score = 25.8 bits (50), Expect(4) = 3e-024
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +2 / +2
Query: 146 KVADFGLSRLYP 181
KVADFGLSR+ P
Sbjct: 257 KVADFGLSRIGP 292
>gb|BF261155.2|BF261155 HVSMEf0023O11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023O11f, mRNA sequence
Length = 748
Score = 71.6 bits (150), Expect(3) = 4e-024
Identities = 29/48 (60%), Positives = 40/48 (83%)
Frame = +2 / +3
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVD 334
THVSTV +G+ GY+DP Y + +LTEKSDVYSFGVVL E++ ++PA++
Sbjct: 588 THVSTVVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLFEVLCARPALN 731
Score = 50.6 bits (104), Expect(3) = 4e-024
Identities = 21/31 (67%), Positives = 24/31 (77%)
Frame = +2 / +3
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
IIHRDVKT NILLD + KV+DFGLS+ P
Sbjct: 483 IIHRDVKTTNILLDEKWVAKVSDFGLSKTGP 575
Score = 27.2 bits (53), Expect(3) = 4e-024
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L+W +R+ I I A L YLH
Sbjct: 402 LSWRQRLEICIGAARGLHYLH 464
>gb|BF256987.2|BF256987 HVSMEf0011I21f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0011I21f, mRNA sequence
Length = 580
Score = 111 bits (237), Expect = 7e-024
Identities = 49/96 (51%), Positives = 66/96 (68%)
Frame = +2 / +2
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
IIHRD+K++NILLDN+F +V+DFGL+RL THV+T GT GY+ P Y KLT
Sbjct: 35 IIHRDIKSSNILLDNNFEAQVSDFGLARLAADSNTHVTTRVMGTFGYLAPEYALSGKLTA 214
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMA 376
KSD+YSFGVVL+EL++ + VD + + +L A
Sbjct: 215 KSDLYSFGVVLLELITGRKPVDSSQPLGDESLVEWA 322
>gb|BE196273.3|BE196273 HVSMEh0091N12f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0091N12f, mRNA sequence
Length = 951
Score = 95.5 bits (202), Expect(2) = 7e-024
Identities = 38/75 (50%), Positives = 56/75 (74%)
Frame = +2 / +2
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+++HRD+K++NIL+D+ F+ KV+DFGL+++ + +H++T GT GYV P Y L
Sbjct: 383 KVVHRDIKSSNILIDDEFNGKVSDFGLAKMLGSDKSHITTRVMGTFGYVAPEYANTGMLN 562
Query: 266 EKSDVYSFGVVLVEL 310
EKSDVYSFGV+L EL
Sbjct: 563 EKSDVYSFGVLLWEL 607
Score = 35.4 bits (71), Expect(2) = 7e-024
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +2 / +2
Query: 20 TWPRRMSIAIETAEALAYLH 79
TW RM + I TA+ALAYLH
Sbjct: 308 TWDNRMKVVIGTAKALAYLH 367
>gb|BJ457712.1|BJ457712 BJ457712 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak28p18 3', mRNA sequence
Length = 708
Score = 111 bits (236), Expect = 9e-024
Identities = 54/164 (32%), Positives = 95/164 (57%)
Frame = +2 / -1
Query: 92 IHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTEK 271
+H DVK++NILLD++ KV+DFG S L P + + T+ QGT GY+DP Y Q +LT+K
Sbjct: 708 LHGDVKSSNILLDDNHKAKVSDFGASILAPTDESQFVTLVQGTCGYLDPEYMQTCQLTDK 529
Query: 272 SDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYETDGET 451
SDVYSFGVVL+EL++ K ++ + +L+ M ++ ++ + + ++D + E + E
Sbjct: 528 SDVYSFGVVLLELLTCKKPFNLDAIGQDKSLSMMFMSAMKENRLEVILDDGIKDEDNMEI 349
Query: 452 KKSIDLVAELAFRCLQLERDSRPSMXXVVEALNRIXNGDSLENN 583
+ I +A+ R S + ++ L ++ + ++ N
Sbjct: 348 LEEIAELAKHCLEMSGENRPSMKEVAEKLDRLRKVMHHPWVQRN 217
>gb|CA014211.1|CA014211 HT10L08r HT Hordeum vulgare subsp. vulgare cDNA clone HT10L08
5-PRIME, mRNA sequence
Length = 640
Score = 111 bits (236), Expect = 9e-024
Identities = 49/97 (50%), Positives = 67/97 (69%)
Frame = +2 / +1
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
E IHRD+K++NILLD F KVADFGL++ THVST GT GY+ P Y KLT
Sbjct: 28 EDIHRDIKSSNILLDFKFEAKVADFGLAKFTSDNNTHVSTRVMGTFGYLAPEYASSGKLT 207
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMA 376
EKSDV+SFGV+L+EL++ + VD +++ + +L + A
Sbjct: 208 EKSDVFSFGVMLLELITGRRPVDSTQTYMDDSLVDWA 318
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 291,691,722
Number of Sequences: 312970
Number of extensions: 5097034
Number of successful extensions: 95908
Number of sequences better than 1.0e-020: 119
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6913923567
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)