TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBB9c12.pg.2.1
(545 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF622661.2|BF622661 HVSMEa0007K11f Hordeum vulgare seedl... 283 8e-076
gb|BF621553.2|BF621553 HVSMEa0011G05f Hordeum vulgare seedl... 262 1e-069
gb|CB859283.1|CB859283 HI09P17w HI Hordeum vulgare subsp. v... 193 1e-048
gb|BI947779.1|BI947779 HVSMEl0006O13f Hordeum vulgare spike... 161 3e-047
gb|AV929760.1|AV929760 AV929760 K. Sato unpublished cDNA li... 111 5e-029
gb|AV927784.1|AV927784 AV927784 K. Sato unpublished cDNA li... 111 5e-029
gb|AV833284.1|AV833284 AV833284 K. Sato unpublished cDNA li... 97 1e-026
gb|CA013515.1|CA013515 HT08L06r HT Hordeum vulgare subsp. v... 111 1e-025
gb|BQ464840.1|BQ464840 HU01M16T HU Hordeum vulgare subsp. v... 98 1e-024
gb|BQ663266.1|BQ663266 HU01M16w HU Hordeum vulgare subsp. v... 98 1e-024
gb|CA015437.1|CA015437 HT14F01r HT Hordeum vulgare subsp. v... 98 1e-024
gb|CA018416.1|CA018416 HV08I24r HV Hordeum vulgare subsp. v... 96 1e-024
gb|BF255286.3|BF255286 HVSMEf0006H07f Hordeum vulgare seedl... 98 5e-024
gb|AV927070.1|AV927070 AV927070 K. Sato unpublished cDNA li... 109 1e-023
gb|AV931900.1|AV931900 AV931900 K. Sato unpublished cDNA li... 109 1e-023
gb|BQ660136.1|BQ660136 HI01C06w HI Hordeum vulgare subsp. v... 91 1e-023
gb|BQ763397.1|BQ763397 EBro02_SQ008_B12_R root, 3 week, hyd... 109 2e-023
gb|BM816261.1|BM816261 HC113F04_SK.ab1 HC Hordeum vulgare s... 93 2e-023
gb|BQ662175.1|BQ662175 HR01O04u HR Hordeum vulgare subsp. v... 108 3e-023
gb|BF260942.2|BF260942 HVSMEf0023F14f Hordeum vulgare seedl... 98 2e-022
gb|BJ450621.1|BJ450621 BJ450621 K. Sato unpublished cDNA li... 104 4e-022
gb|BJ458043.1|BJ458043 BJ458043 K. Sato unpublished cDNA li... 104 4e-022
gb|CA001229.1|CA001229 HS18N07u HS Hordeum vulgare subsp. v... 104 4e-022
gb|CA000677.1|CA000677 HS07F12u HS Hordeum vulgare subsp. v... 103 7e-022
gb|BM816260.1|BM816260 HC113E04_SK.ab1 HC Hordeum vulgare s... 93 9e-022
gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedl... 79 2e-021
gb|BI957863.1|BI957863 HVSMEn0011M19f Hordeum vulgare rachi... 82 5e-021
gb|BJ463253.1|BJ463253 BJ463253 K. Sato unpublished cDNA li... 97 6e-021
>gb|BF622661.2|BF622661 HVSMEa0007K11f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0007K11f, mRNA sequence
Length = 697
Score = 283 bits (612), Expect = 8e-076
Identities = 106/150 (70%), Positives = 127/150 (84%)
Frame = +2 / +2
Query: 5 ELGNGLYNSGKPFIWVVRSNEEHKLSNELRDKCKERGLIVSWCPQLEVLAHKATGCFFTH 184
ELGNGL NS KPF+WVVRSNEEHKLS EL++KC + GLI SWCPQLEVLAH+A GCF TH
Sbjct: 122 ELGNGLCNSSKPFLWVVRSNEEHKLSEELKEKCGKIGLIGSWCPQLEVLAHRAIGCFITH 301
Query: 185 CGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWGLGVRVRKDEKGLVTRDEVERCIK 364
CGWNSTLEA+VNGVP IPHWADQPTI+KY+ES WG+GVR RK++ G + ++EV+RC+
Sbjct: 302 CGWNSTLEALVNGVPFVGIPHWADQPTIAKYVESAWGMGVRARKNKIGCLKKEEVDRCLI 481
Query: 365 DVMDGDSKDKYRKSATMWMQKAKAAMQNGG 454
+VMDG+ KDKY+K+A WMQK + AMQ GG
Sbjct: 482 EVMDGERKDKYKKNAMNWMQKGQEAMQEGG 571
Score = 168 bits (361), Expect(2) = 5e-044
Identities = 71/108 (65%), Positives = 74/108 (68%)
Frame = -3 / -2
Query: 327 PFSSLRTRTPKPHMLSIYFDMVGWSAQCGIAAIGTPLTIASNVEFHPQCVKKQPVALCAR 148
P R RTP PH LS YF MVGWSAQCGI GTPLT AS+VEFHPQ V KQP+ALCAR
Sbjct: 444 PILFFRARTPIPHALSTYFAMVGWSAQCGIPTKGTPLTSASSVEFHPQWVMKQPIALCAR 265
Query: 147 TSSWGHQETIXXXXXXXXXXXLDNLCSSFDLTTQMNGLPELYNPLPSS 4
TSSWGH E I DNLCSS DLTTQ GL EL+ PLPSS
Sbjct: 264 TSSWGHHEPINPIFPHFSLSSSDNLCSSLDLTTQRKGLLELHKPLPSS 121
Score = 98.2 bits (208), Expect(2) = 4e-021
Identities = 50/118 (42%), Positives = 64/118 (54%)
Frame = -2 / -1
Query: 358 APFHLVPRYQAFLILAHSDTQAPHALHIF*YGRLVCPVWYCRHWHTINNCFQCRVPSAMC 179
AP +L+ + L+ + T P LHI G LVCP+W TIN C C VPS +
Sbjct: 475 APINLLLL*TSNLVFPCTHTHTPCTLHILCNGGLVCPMWNTHKRDTINKCL*C*VPSTVG 296
Query: 178 EETTCSFMC*NFQLGAPRNNKATFLALVTKFIGQLVFFV*PHNPNEWFARIVQSIAKL 5
E T S MC N +LGAP + F +F G LVF + +NP + FARI Q+IAKL
Sbjct: 295 NEATYSPMCKNLKLGAP*AY*SNFPTFFFEFFG*LVFLIGSYNPKKRFARIAQTIAKL 122
Score = 94.5 bits (200), Expect(2) = 3e-025
Identities = 48/108 (44%), Positives = 60/108 (55%)
Frame = +3 / +3
Query: 129 GAPSWKF*HIKLQVVSSHIADGTRHWKQLLMVCQWRQYHTGQTSRPYQNIWRACGAWVSE 308
GAPS +F HI L V S DGT+H + LLMV +H GQT+ P Q++WR G WV
Sbjct: 246 GAPSLRFLHIGL*VASLPTVDGTQH*RHLLMVSLLWVFHIGQTNPPLQSMWRVHGVWVCV 425
Query: 309 CARMRKAW*RGTRWKGASRMSWMVIVRISIGRVPLCGCKRPRQLCRME 452
+ R *R R GA WM RIS ++P GCKR R+ C+ E
Sbjct: 426 HGKTRLDV*RRRRLIGAL*K*WMGRERISTKKMP*TGCKRARRQCKKE 569
Score = 73.9 bits (155), Expect(3) = 1e-022
Identities = 32/64 (50%), Positives = 38/64 (59%)
Frame = +1 / +1
Query: 112 WPYCFLVPPAGSSST*SYRLFLHTLRMELDIGSNC*WCANGGNTTLGRPADHIKIYGEHV 291
W +VPPA S T* YRL + L MEL+ C*WC G +TLGRP H K+ GE +
Sbjct: 229 WINRLMVPPA*GSCT*GYRLLHYPLWMELNTRGTC*WCPFCGYSTLGRPTHHCKVCGECM 408
Query: 292 GLGC 303
G GC
Sbjct: 409 GYGC 420
Score = 40.0 bits (81), Expect(2) = 3e-025
Identities = 13/20 (65%), Positives = 16/20 (80%)
Frame = +1 / +1
Query: 4 GAWQWIVQFWQTIHLGCEVK 63
GAWQW VQF QT LGC+++
Sbjct: 121 GAWQWFVQF*QTFSLGCKIQ 180
Score = 38.2 bits (77), Expect(3) = 1e-022
Identities = 18/28 (64%), Positives = 19/28 (67%)
Frame = +3 / +3
Query: 6 SLAMDCTILANHSFGL*GQTKNTSCPMN 89
SLAM C ILAN FGL* +NTS P N
Sbjct: 123 SLAMVCAILANLFFGL*DPMRNTSYPKN 206
Score = 31.3 bits (62), Expect(3) = 1e-022
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = +1 / +1
Query: 373 GW***G*V*EECHYVDAKGQGSYAEWREL 459
GW G*V ++CH +DAKG G A R++
Sbjct: 490 GWGEKG*VQKKCHELDAKGPGGNARRRKV 576
Score = 29.0 bits (57), Expect(2) = 5e-044
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3 / -2
Query: 453 PPFCIAALAFCIHIVALF 400
PP CIA+ FCI +A F
Sbjct: 570 PPSCIASWPFCIQFMAFF 517
Score = 22.1 bits (42), Expect(2) = 4e-021
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = -1 / -3
Query: 434 PWPFAST*WH 405
P PFAS+ WH
Sbjct: 551 PGPFASSSWH 522
>gb|BF621553.2|BF621553 HVSMEa0011G05f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011G05f, mRNA sequence
Length = 797
Score = 262 bits (567), Expect = 1e-069
Identities = 99/139 (71%), Positives = 119/139 (85%)
Frame = +2 / +3
Query: 68 EHKLSNELRDKCKERGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPH 247
EHKLS EL++KC + GLIVSWCPQLEVLAH+A GCF THCGWNSTLEA+VNGVP IPH
Sbjct: 3 EHKLSEELKEKCGKIGLIVSWCPQLEVLAHRAIGCFVTHCGWNSTLEALVNGVPFVGIPH 182
Query: 248 WADQPTISKYMESMWGLGVRVRKDEKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQK 427
WADQPTI+KY+ES WG+GVR RK++ G + ++EVERCI +VMDG+ KD+Y+K+A WMQK
Sbjct: 183 WADQPTIAKYVESAWGMGVRARKNKNGCLKKEEVERCIIEVMDGERKDEYKKNAMNWMQK 362
Query: 428 AKAAMQNGGSSAKNITEFA 484
AK AMQ GGSS K++TEFA
Sbjct: 363 AKEAMQEGGSSDKHVTEFA 419
Score = 184 bits (396), Expect = 4e-046
Identities = 79/145 (54%), Positives = 85/145 (58%)
Frame = -3 / -2
Query: 501 LKEYFAANSVIFLAELPPFCIAALAFCIHIVALFXXXXXXXXXXXXXXXXXXXXLVTKPF 322
+ EY ANSV L+ELPP CIA+LAFCI +A F PF
Sbjct: 436 IAEYLVANSVTCLSELPPSCIASLAFCIQFMAFFLYSSFLSPSITSIMHLSTSSFFRHPF 257
Query: 321 SSLRTRTPKPHMLSIYFDMVGWSAQCGIAAIGTPLTIASNVEFHPQCVKKQPVALCARTS 142
R RTP PH LS YF MVGWSAQCGI GTPLT AS+VEFHPQ V KQP+ALCARTS
Sbjct: 256 LFFRARTPIPHALSTYFAMVGWSAQCGIPTKGTPLTSASSVEFHPQWVTKQPIALCARTS 77
Query: 141 SWGHQETIXXXXXXXXXXXLDNLCS 67
SWGH ETI DNLCS
Sbjct: 76 SWGHHETINPIFPHFSLSSSDNLCS 2
Score = 107 bits (229), Expect = 4e-023
Identities = 54/128 (42%), Positives = 67/128 (52%)
Frame = +1 / +2
Query: 112 WPYCFLVPPAGSSST*SYRLFLHTLRMELDIGSNC*WCANGGNTTLGRPADHIKIYGEHV 291
W +VPPA S T* YRL + L MEL+ C*WC G +TLGRP H K+ GE +
Sbjct: 47 WINSLMVPPA*GSCT*GYRLLRYPLWMELNTRGTC*WCPFCGYSTLGRPTHHCKVCGECM 226
Query: 292 GLGCPSAQXXXXXXXXXXXXKVHQGCHGW***G*V*EECHYVDAKGQGSYAEWRELGQEY 471
G GC + +VH GW G*V ++CH +DAKGQG A R+ Q
Sbjct: 227 GYGCACTEKQEWMSKEGGG*EVHYRGDGWGEKG*VQKKCHELDAKGQGGNARRRKFRQAC 406
Query: 472 YRIRREVF 495
IR +VF
Sbjct: 407 N*IRYQVF 430
Score = 101 bits (216), Expect = 2e-021
Identities = 57/151 (37%), Positives = 72/151 (47%)
Frame = -2 / -1
Query: 520 EIFEKLT*RILRGEFCNILGRAPSILHSCLGLLHPHSGXXXXXXXXXXXXXXLDAPFHLV 341
+I + L RIL EF +L S LH LGLLHP G +AP +L+
Sbjct: 455 DIIQVLYCRILGSEFSYMLV*TSSFLHCLLGLLHPVHGIFFVLILSLPIHHLYNAPLNLL 276
Query: 340 PRYQAFLILAHSDTQAPHALHIF*YGRLVCPVWYCRHWHTINNCFQCRVPSAMCEETTCS 161
+ L+ + T P LHI G LVCP+W TIN C C VPS + E T S
Sbjct: 275 LL*TSILVFPCTHTHTPCTLHILCNGGLVCPMWNTHKRDTINKCL*C*VPSTVGNEATYS 96
Query: 160 FMC*NFQLGAPRNNKATFLALVTKFIGQLVF 68
MC N +LGAP + + F +F G LVF
Sbjct: 95 PMCKNLKLGAP*DY*SNFPTFFFEFFG*LVF 3
Score = 101 bits (216), Expect = 2e-021
Identities = 52/122 (42%), Positives = 68/122 (55%)
Frame = +3 / +1
Query: 129 GAPSWKF*HIKLQVVSSHIADGTRHWKQLLMVCQWRQYHTGQTSRPYQNIWRACGAWVSE 308
GAPS +F HI L V S DGT+H + LLMV +H GQT+ P Q++WR G WV
Sbjct: 64 GAPSLRFLHIGL*VASLPTVDGTQH*RHLLMVSLLWVFHIGQTNPPLQSMWRVHGVWVCV 243
Query: 309 CARMRKAW*RGTRWKGASRMSWMVIVRISIGRVPLCGCKRPRQLCRMEGARPRILQNSPR 488
+ R *R R +GA WM R+S ++P GCKRPR+ C+ E + + NS
Sbjct: 244 HGKTRMDV*RRRRLRGAL*R*WMGRERMSTKKMP*TGCKRPRRQCKKEEVQTSM*LNSLP 423
Query: 489 SI 494
SI
Sbjct: 424 SI 429
>gb|CB859283.1|CB859283 HI09P17w HI Hordeum vulgare subsp. vulgare cDNA clone HI09P17
3-PRIME, mRNA sequence
Length = 560
Score = 193 bits (415), Expect = 1e-048
Identities = 76/107 (71%), Positives = 87/107 (81%)
Frame = +2 / +1
Query: 167 GCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWGLGVRVRKDEKGLVTRDE 346
GCF THCGWNST EA+V GVPM A+P ADQPT +KY+ES WG GVR+R DEKGLV R+E
Sbjct: 142 GCFLTHCGWNSTTEALVAGVPMVAMPRSADQPTTAKYVESAWGTGVRIRTDEKGLVRREE 321
Query: 347 VERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNITEFAA 487
VERCI+ VMDG+ K +YRK+AT WM+ AK AMQ GGSS KNI EFAA
Sbjct: 322 VERCIRKVMDGEEKVEYRKNATKWMRMAKEAMQEGGSSDKNIAEFAA 462
Score = 142 bits (304), Expect = 2e-033
Identities = 64/112 (57%), Positives = 70/112 (62%)
Frame = -3 / -1
Query: 501 LKEYFAANSVIFLAELPPFCIAALAFCIHIVALFXXXXXXXXXXXXXXXXXXXXLVTKPF 322
L +Y AANS IFL+ELPP CIA+LA IH VA L+T PF
Sbjct: 476 LDKYLAANSAIFLSELPPSCIASLAILIHFVAFLRYSTFSSPSITFLMHLSTSSLLTNPF 297
Query: 321 SSLRTRTPKPHMLSIYFDMVGWSAQCGIAAIGTPLTIASNVEFHPQCVKKQP 166
SS+R RTP PH LS YF +VGWSA GIA IGTP T AS VEFHPQ VKKQP
Sbjct: 296 SSVRIRTPVPHALSTYFAVVGWSADRGIATIGTPATSASVVEFHPQ*VKKQP 141
>gb|BI947779.1|BI947779 HVSMEl0006O13f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0006O13f, mRNA sequence
Length = 1003
Score = 161 bits (347), Expect(2) = 3e-047
Identities = 57/88 (64%), Positives = 75/88 (85%)
Frame = +2 / +1
Query: 146 VLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWGLGVRVRKDEK 325
VL+HKATGCF THCGWNST EAIV GVP+ A+P W DQPT +KY+ES WG+GVRV +D++
Sbjct: 1 VLSHKATGCFLTHCGWNSTTEAIVTGVPLLAMPQWTDQPTTAKYIESAWGIGVRVHRDKE 180
Query: 326 GLVTRDEVERCIKDVMDGDSKDKYRKSA 409
G+V ++EVERCIK+V+DG+ K +Y K++
Sbjct: 181 GIVRKEEVERCIKEVLDGERKQEYMKNS 264
Score = 84.0 bits (177), Expect(2) = 4e-028
Identities = 40/59 (67%), Positives = 43/59 (72%)
Frame = -3 / -3
Query: 333 TKPFSSLRTRTPKPHMLSIYFDMVGWSAQCGIAAIGTPLTIASNVEFHPQCVKKQPVAL 157
T P S TRTP PH LS+YF +VG S GIA GTP+TIAS VEFHPQ VKKQPVAL
Sbjct: 188 TIPSLSRCTRTPIPHALSMYFAVVG*SVH*GIAKSGTPVTIASVVEFHPQ*VKKQPVAL 12
Score = 60.2 bits (125), Expect(2) = 4e-028
Identities = 23/32 (71%), Positives = 27/32 (84%)
Frame = -3 / -2
Query: 495 EYFAANSVIFLAELPPFCIAALAFCIHIVALF 400
EY AANS IFL+ELPP CIA+LAF IHI+ +F
Sbjct: 351 EYLAANSAIFLSELPPLCIASLAFVIHIIRIF 256
Score = 46.4 bits (95), Expect(2) = 3e-047
Identities = 20/29 (68%), Positives = 20/29 (68%)
Frame = +2 / +2
Query: 401 KSATMWMQKAKAAMQNGGSSAKNITEFAA 487
K MWM KAK AM GGSS KNI EFAA
Sbjct: 257 KILIMWMTKAKEAMHKGGSSDKNIAEFAA 343
>gb|AV929760.1|AV929760 AV929760 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd16h10 3', mRNA sequence
Length = 669
Score = 111 bits (238), Expect(2) = 5e-029
Identities = 37/88 (42%), Positives = 61/88 (69%)
Frame = +2 / -3
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESM 289
+GL+V W PQ VL+H + CF +HCGWNST+E + +GVP P++ADQ Y+ ++
Sbjct: 373 KGLVVGWAPQQRVLSHPSVACFVSHCGWNSTMEGLRHGVPFLCWPYFADQFCNQSYICNV 194
Query: 290 WGLGVRVRKDEKGLVTRDEVERCIKDVM 373
WG GV++ DE+G+VT++E++ + ++
Sbjct: 193 WGTGVKIHADERGVVTKEEIKNKVAQLL 110
Score = 35.4 bits (71), Expect(2) = 5e-029
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = +2 / -3
Query: 5 ELGNGLYNSGKPFIWVVRSNEEHKLSNELRDKCKER 112
EL +GL +G+PF+W VR+N + + D K R
Sbjct: 490 ELADGLELTGRPFLWTVRTNFTTGIGEDWLDAFKRR 383
>gb|AV927784.1|AV927784 AV927784 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3k12 3', mRNA sequence
Length = 651
Score = 111 bits (238), Expect(2) = 5e-029
Identities = 37/88 (42%), Positives = 61/88 (69%)
Frame = +2 / -1
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESM 289
+GL+V W PQ VL+H + CF +HCGWNST+E + +GVP P++ADQ Y+ ++
Sbjct: 360 KGLVVGWAPQQRVLSHPSVACFVSHCGWNSTMEGLRHGVPFLCWPYFADQFCNQSYICNV 181
Query: 290 WGLGVRVRKDEKGLVTRDEVERCIKDVM 373
WG GV++ DE+G+VT++E++ + ++
Sbjct: 180 WGTGVKIHADERGVVTKEEIKNKVAQLL 97
Score = 35.4 bits (71), Expect(2) = 5e-029
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = +2 / -1
Query: 5 ELGNGLYNSGKPFIWVVRSNEEHKLSNELRDKCKER 112
EL +GL +G+PF+W VR+N + + D K R
Sbjct: 477 ELADGLELTGRPFLWTVRTNFTTGIGEDWLDAFKRR 370
>gb|AV833284.1|AV833284 AV833284 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags5b03, mRNA sequence
Length = 671
Score = 96.8 bits (205), Expect(3) = 1e-026
Identities = 38/92 (41%), Positives = 55/92 (59%)
Frame = +2 / +1
Query: 50 VVRSNEEHKLSNELRDKCKERGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVP 229
+V+ ++E L E + R ++ +WCPQ +VL +A G F TH GWNS+LE I GVP
Sbjct: 250 LVKGDDEAALPPEFSTATEGRSMLSTWCPQEKVLEQEAVGVFLTHSGWNSSLEGICGGVP 429
Query: 230 MAAIPHWADQPTISKYMESMWGLGVRVRKDEK 325
M P +ADQ T +Y + WG+G+ + D K
Sbjct: 430 MVCWPFFADQQTNCRYKCTEWGIGMEIGDDVK 525
Score = 36.8 bits (74), Expect(3) = 1e-026
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +2 / +1
Query: 332 VTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNI 472
V R EVE I++ M+GD + R+ + A A+ + GG S +N+
Sbjct: 520 VKRTEVEALIREAMEGDKGREMRRRVLELWESAVASARPGGRSMRNV 660
Score = 23.1 bits (44), Expect(3) = 1e-026
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = +2 / +1
Query: 5 ELGNGLYNSGKPFIWVVR 58
E GL N+G F+W VR
Sbjct: 190 EFAWGLANTGYAFLWNVR 243
>gb|CA013515.1|CA013515 HT08L06r HT Hordeum vulgare subsp. vulgare cDNA clone HT08L06
5-PRIME, mRNA sequence
Length = 634
Score = 111 bits (237), Expect(2) = 1e-025
Identities = 37/88 (42%), Positives = 61/88 (69%)
Frame = +2 / +1
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESM 289
+GL+V W PQ VL+H + CF +HCGWNST+E + +GVP P++ADQ Y+ ++
Sbjct: 253 KGLVVGWAPQQRVLSHPSIACFVSHCGWNSTMEGLRHGVPFLCWPYFADQFCNQSYICNV 432
Query: 290 WGLGVRVRKDEKGLVTRDEVERCIKDVM 373
WG GV++ DE+G+VT++E++ + ++
Sbjct: 433 WGTGVKIHADERGVVTKEEIKNKVXQLL 516
Score = 24.4 bits (47), Expect(2) = 1e-025
Identities = 9/26 (34%), Positives = 13/26 (50%)
Frame = +2 / +1
Query: 35 KPFIWVVRSNEEHKLSNELRDKCKER 112
+PF+W VR N + + D K R
Sbjct: 166 RPFLWAVRPNITTGIGEDWLDAFKRR 243
>gb|BQ464840.1|BQ464840 HU01M16T HU Hordeum vulgare subsp. vulgare cDNA clone HU01M16
5-PRIME, mRNA sequence
Length = 571
Score = 97.7 bits (207), Expect(2) = 1e-024
Identities = 32/52 (61%), Positives = 40/52 (76%)
Frame = +2 / +1
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPT 265
RG++V WC Q+ VL+H A GCF THCGWNSTLEA+ G P+ A+P W+DQ T
Sbjct: 118 RGMVVGWCDQVRVLSHPAVGCFVTHCGWNSTLEAVACGAPVVAVPQWSDQDT 273
Score = 35.0 bits (70), Expect(2) = 1e-024
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2 / +1
Query: 290 WGLGVRVRKDEKGLVTRDEVERCIKDVMDG 379
WG+GVR D L+ +E+ RC++ +M G
Sbjct: 295 WGVGVRAAADVDRLLVAEELARCLEMIMGG 384
>gb|BQ663266.1|BQ663266 HU01M16w HU Hordeum vulgare subsp. vulgare cDNA clone HU01M16
3-PRIME, mRNA sequence
Length = 570
Score = 97.7 bits (207), Expect(2) = 1e-024
Identities = 32/52 (61%), Positives = 40/52 (76%)
Frame = +2 / -1
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPT 265
RG++V WC Q+ VL+H A GCF THCGWNSTLEA+ G P+ A+P W+DQ T
Sbjct: 453 RGMVVGWCDQVRVLSHPAVGCFVTHCGWNSTLEAVACGAPVVAVPQWSDQDT 298
Score = 35.0 bits (70), Expect(2) = 1e-024
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2 / -1
Query: 290 WGLGVRVRKDEKGLVTRDEVERCIKDVMDG 379
WG+GVR D L+ +E+ RC++ +M G
Sbjct: 276 WGVGVRAAADVDRLLVAEELARCLEMIMGG 187
>gb|CA015437.1|CA015437 HT14F01r HT Hordeum vulgare subsp. vulgare cDNA clone HT14F01
5-PRIME, mRNA sequence
Length = 510
Score = 97.7 bits (207), Expect(2) = 1e-024
Identities = 32/52 (61%), Positives = 40/52 (76%)
Frame = +2 / +1
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPT 265
RG++V WC Q+ VL+H A GCF THCGWNSTLEA+ G P+ A+P W+DQ T
Sbjct: 157 RGMVVGWCDQVRVLSHPAVGCFVTHCGWNSTLEAVACGAPVVAVPQWSDQDT 312
Score = 35.0 bits (70), Expect(2) = 1e-024
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2 / +1
Query: 290 WGLGVRVRKDEKGLVTRDEVERCIKDVMDG 379
WG+GVR D L+ +E+ RC++ +M G
Sbjct: 334 WGVGVRAAADVDRLLVAEELARCLEMIMGG 423
>gb|CA018416.1|CA018416 HV08I24r HV Hordeum vulgare subsp. vulgare cDNA clone HV08I24
5-PRIME, mRNA sequence
Length = 647
Score = 96.4 bits (204), Expect(3) = 1e-024
Identities = 37/78 (47%), Positives = 49/78 (62%)
Frame = +2 / +3
Query: 77 LSNELRDKCKERGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWAD 256
L E + RGL+ SWCPQ EVL H A G F TH GWNS LE++ GVP+ + P +AD
Sbjct: 75 LPEEFLAETAGRGLMASWCPQQEVLNHPAVGAFLTHSGWNSALESLFGGVPVISWPFFAD 254
Query: 257 QPTISKYMESMWGLGVRV 310
Q T +Y + WG+G+ +
Sbjct: 255 QQTNCRYQCNEWGVGMEI 308
Score = 30.4 bits (60), Expect(3) = 1e-024
Identities = 14/36 (38%), Positives = 17/36 (47%)
Frame = +2 / +1
Query: 371 MDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNITE 478
M+G+ RK A W + A A GGSS N E
Sbjct: 358 MEGEKGKSMRKRAVEWKESAVKAAMPGGSSHINFHE 465
Score = 23.1 bits (44), Expect(3) = 1e-024
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +2 / +3
Query: 29 SGKPFIWVVR 58
SGK F+W+VR
Sbjct: 15 SGKQFMWIVR 44
>gb|BF255286.3|BF255286 HVSMEf0006H07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0006H07f, mRNA sequence
Length = 505
Score = 97.7 bits (207), Expect(2) = 5e-024
Identities = 32/52 (61%), Positives = 40/52 (76%)
Frame = +2 / +2
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPT 265
RG++V WC Q+ VL+H A GCF THCGWNSTLEA+ G P+ A+P W+DQ T
Sbjct: 38 RGMVVGWCDQVRVLSHPAVGCFVTHCGWNSTLEAVACGAPVVAVPQWSDQDT 193
Score = 32.7 bits (65), Expect(2) = 5e-024
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +2 / +2
Query: 290 WGLGVRVRKDEKGLVTRDEVERCIKDVM 373
WG+GVR D L+ +E+ RC++ +M
Sbjct: 215 WGVGVRAATDVDRLLVAEELARCLEMIM 298
>gb|AV927070.1|AV927070 AV927070 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26k01 5', mRNA sequence
Length = 676
Score = 109 bits (233), Expect = 1e-023
Identities = 47/122 (38%), Positives = 69/122 (56%)
Frame = +2 / +3
Query: 116 LIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWG 295
++ SW PQ++VL H+ATG F THCGWNS LEA+ GVPM +P A+Q M G
Sbjct: 138 VVKSWAPQVDVLRHRATGAFVTHCGWNSVLEAVAAGVPMLCLPLEAEQKMNKVCMTEDMG 317
Query: 296 LGVRVRKDEKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNIT 475
+ V + G V +EVE ++ V++G + R ++AKAA++ GSS +
Sbjct: 318 VAVELEGYMAGFVEAEEVEAKVRLVIEGGDGRQLRARVAARREEAKAALEEDGSSRTSFA 497
Query: 476 EF 481
+F
Sbjct: 498 QF 503
>gb|AV931900.1|AV931900 AV931900 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26k01 3', mRNA sequence
Length = 631
Score = 109 bits (233), Expect = 1e-023
Identities = 47/122 (38%), Positives = 69/122 (56%)
Frame = +2 / -1
Query: 116 LIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWG 295
++ SW PQ++VL H+ATG F THCGWNS LEA+ GVPM +P A+Q M G
Sbjct: 505 VVKSWAPQVDVLRHRATGAFVTHCGWNSVLEAVAAGVPMLCLPLEAEQKMNKVCMTEDMG 326
Query: 296 LGVRVRKDEKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNIT 475
+ V + G V +EVE ++ V++G + R ++AKAA++ GSS +
Sbjct: 325 VAVELEGYMAGFVEAEEVEAKVRLVIEGGDGRQLRARVAARREEAKAALEEDGSSRTSFA 146
Query: 476 EF 481
+F
Sbjct: 145 QF 140
>gb|BQ660136.1|BQ660136 HI01C06w HI Hordeum vulgare subsp. vulgare cDNA clone HI01C06
3-PRIME, mRNA sequence
Length = 668
Score = 90.9 bits (192), Expect(2) = 1e-023
Identities = 36/70 (51%), Positives = 44/70 (62%)
Frame = +2 / -3
Query: 101 CKERGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYM 280
C RGL+V WC Q+EVLAH A G F THCGWNS LE++ GVPM P DQ T + +
Sbjct: 639 CAGRGLVVPWCCQVEVLAHAALGGFLTHCGWNSVLESVWAGVPMLCFPLLTDQFTNRRLV 460
Query: 281 ESMWGLGVRV 310
W +GV +
Sbjct: 459 VREWRVGVPI 430
Score = 38.2 bits (77), Expect(2) = 1e-023
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = +2 / -3
Query: 320 EKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNITEF 481
++G V DEV I+ V+ G+ + R++ K KAA+ GGSS ++ +F
Sbjct: 426 DRGKVFADEVAARIQGVISGEEGQQLRQALKKVRAKLKAAVAPGGSSQRSFDDF 265
>gb|BQ763397.1|BQ763397 EBro02_SQ008_B12_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_B12 5', mRNA sequence
Length = 552
Score = 109 bits (232), Expect = 2e-023
Identities = 45/115 (39%), Positives = 70/115 (60%)
Frame = +2 / +3
Query: 116 LIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWG 295
++ SW PQ +VLAH A GCF THCGWNS LE+++ GVPM A P +A+Q + ++E
Sbjct: 135 VVKSWAPQRDVLAHDAVGCFVTHCGWNSVLESVMAGVPMLAWPLYAEQRMNAVFLEKEME 314
Query: 296 LGVRVRKDEKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSS 460
L V + ++ +V +EV + ++ +MD D R+ M++A+ A+ GG S
Sbjct: 315 LAVAMEGYDREMVEAEEVAKKVRWMMDSDGGRVLRERTLTVMRRAEEALLEGGES 479
>gb|BM816261.1|BM816261 HC113F04_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC113F04_SK.ab1 similar to
Galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase, mRNA sequence
Length = 935
Score = 93.2 bits (197), Expect(2) = 2e-023
Identities = 36/101 (35%), Positives = 59/101 (58%)
Frame = +2 / +3
Query: 17 GLYNSGKPFIWVVRSNEEHKLSNELRDKCKERGLIVSWCPQLEVLAHKATGCFFTHCGWN 196
G+ + G +V+ ++ L E ++R ++ +WCPQ +VL H+A G F TH GWN
Sbjct: 12 GIRHEGNVRPDLVKGHDNAVLPPEFSAATEDRSMLSTWCPQEKVLEHEAIGVFLTHSGWN 191
Query: 197 STLEAIVNGVPMAAIPHWADQPTISKYMESMWGLGVRVRKD 319
S+LE I GVPM P + +Q T +Y + WG+G+ + ++
Sbjct: 192 SSLEGICGGVPMVCWPFFTEQQTNCRYKCTEWGIGMEIGEE 314
Score = 35.4 bits (71), Expect(2) = 2e-023
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +2 / +3
Query: 332 VTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNI 472
VTR EVE +++ M+G + R+ + A A+ ++ G S +N+
Sbjct: 315 VTRTEVEAILREAMEGGKGQEMRRRVLKLQENAVASARHDGRSMRNV 455
>gb|BQ662175.1|BQ662175 HR01O04u HR Hordeum vulgare subsp. vulgare cDNA clone HR01O04
3-PRIME, mRNA sequence
Length = 532
Score = 108 bits (230), Expect = 3e-023
Identities = 42/88 (47%), Positives = 57/88 (64%)
Frame = +2 / -2
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESM 289
+G++V WC Q EVL+H + G F THCGWNSTLEA+ GVP+ A P DQPT + +E
Sbjct: 531 KGMVVEWCDQPEVLSHPSVG*FVTHCGWNSTLEAMALGVPVVAAPSMFDQPTNAMLIEEE 352
Query: 290 WGLGVRVRKDEKGLVTRDEVERCIKDVM 373
W GVR ++ G+ E+ RC++ VM
Sbjct: 351 WAAGVRGDRNGDGIFAGAELARCVELVM 268
>gb|BF260942.2|BF260942 HVSMEf0023F14f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023F14f, mRNA sequence
Length = 816
Score = 97.7 bits (207), Expect(2) = 2e-022
Identities = 35/88 (39%), Positives = 55/88 (62%)
Frame = +2 / +3
Query: 50 VVRSNEEHKLSNELRDKCKERGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVP 229
+V +++ L + +R K + +V W PQ +VL H+A GCF TH GWNSTLE++ GVP
Sbjct: 135 MVGASQNAVLQDAIRTAGKSKARVVEWAPQRDVLRHRAVGCFLTHAGWNSTLESVAEGVP 314
Query: 230 MAAIPHWADQPTISKYMESMWGLGVRVR 313
P + DQ S++M ++WG G+ ++
Sbjct: 315 TVCWPFFVDQQINSRFMGAVWGSGLDMK 398
Score = 27.6 bits (54), Expect(2) = 2e-022
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +2 / +3
Query: 5 ELGNGLYNSGKPFIWVVR 58
E +GL ++G PF+WV+R
Sbjct: 75 EFLSGLVSAGHPFLWVLR 128
>gb|BJ450621.1|BJ450621 BJ450621 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak30g17 5', mRNA sequence
Length = 616
Score = 104 bits (222), Expect = 4e-022
Identities = 48/122 (39%), Positives = 68/122 (55%)
Frame = +2 / +1
Query: 116 LIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWG 295
++ SW PQ+EVL H+ATG F THCGWNSTLE I G+P+ P +A+Q ++
Sbjct: 88 VVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPLYAEQRVNKVHIVEGMK 267
Query: 296 LGVRVRKDEKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNIT 475
LGV +R + LV EVE ++ VM + + R+ T A A++ GGSS
Sbjct: 268 LGVEMRGYNEELVKGVEVEEKVRWVMASEGGNALRERVTAAKVAAAEALKEGGSSYLAFV 447
Query: 476 EF 481
+F
Sbjct: 448 QF 453
>gb|BJ458043.1|BJ458043 BJ458043 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak30g17 3', mRNA sequence
Length = 551
Score = 104 bits (222), Expect = 4e-022
Identities = 48/122 (39%), Positives = 68/122 (55%)
Frame = +2 / -3
Query: 116 LIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWG 295
++ SW PQ+EVL H+ATG F THCGWNSTLE I G+P+ P +A+Q ++
Sbjct: 486 VVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPLYAEQRVNKVHIVEGMK 307
Query: 296 LGVRVRKDEKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNIT 475
LGV +R + LV EVE ++ VM + + R+ T A A++ GGSS
Sbjct: 306 LGVEMRGYNEELVKGVEVEEKVRWVMASEGGNALRERVTAAKVAAAEALKEGGSSYLAFV 127
Query: 476 EF 481
+F
Sbjct: 126 QF 121
>gb|CA001229.1|CA001229 HS18N07u HS Hordeum vulgare subsp. vulgare cDNA clone HS18N07
3-PRIME, mRNA sequence
Length = 544
Score = 104 bits (222), Expect = 4e-022
Identities = 48/122 (39%), Positives = 68/122 (55%)
Frame = +2 / -2
Query: 116 LIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWG 295
++ SW PQ+EVL H+ATG F THCGWNSTLE I G+P+ P +A+Q ++
Sbjct: 432 VVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPLYAEQRVNKVHIVEGMK 253
Query: 296 LGVRVRKDEKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNIT 475
LGV +R + LV EVE ++ VM + + R+ T A A++ GGSS
Sbjct: 252 LGVEMRGYNEELVKGVEVEEKVRWVMASEGGNALRERVTAAKVAAAEALKEGGSSYLAFV 73
Query: 476 EF 481
+F
Sbjct: 72 QF 67
>gb|CA000677.1|CA000677 HS07F12u HS Hordeum vulgare subsp. vulgare cDNA clone HS07F12
3-PRIME, mRNA sequence
Length = 556
Score = 103 bits (220), Expect = 7e-022
Identities = 46/122 (37%), Positives = 68/122 (55%)
Frame = +2 / -2
Query: 116 LIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWG 295
++ SW PQ++VL H+ATG F THCGWNSTLE I G+P+ P +A+Q ++
Sbjct: 531 VVKSWAPQVDVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPLYAEQRVNKVHIVDGMK 352
Query: 296 LGVRVRKDEKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNIT 475
LGV +R + +V +EVE ++ VM + R+ T A A++ GGSS
Sbjct: 351 LGVEMRGYNEEVVKAEEVEEKVRWVMASEGGKALRERVTAAKDAAADALKEGGSSYLAFD 172
Query: 476 EF 481
+F
Sbjct: 171 QF 166
>gb|BM816260.1|BM816260 HC113E04_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC113E04_SK.ab1 similar to
Galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase, mRNA sequence
Length = 842
Score = 92.7 bits (196), Expect(3) = 9e-022
Identities = 34/90 (37%), Positives = 55/90 (61%)
Frame = +2 / +2
Query: 50 VVRSNEEHKLSNELRDKCKERGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVP 229
+V+ ++ L E ++R ++ +WCPQ +VL H+A G F TH GWNS+LE I GVP
Sbjct: 218 LVKGHDNAVLPPEFSAATEDRSMLSTWCPQEKVLEHEAIGVFLTHSGWNSSLEGICGGVP 397
Query: 230 MAAIPHWADQPTISKYMESMWGLGVRVRKD 319
M P + +Q T +Y + WG+G+ + ++
Sbjct: 398 MVCWPFFTEQQTNCRYKCTEWGIGMEIGEE 487
Score = 24.4 bits (47), Expect(3) = 9e-022
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +2 / +2
Query: 5 ELGNGLYNSGKPFIWVVR 58
E GL NSG F+W VR
Sbjct: 158 EFAWGLANSGYTFLWNVR 211
Score = 23.1 bits (44), Expect(3) = 9e-022
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +2 / +2
Query: 332 VTRDEVERCIKDVMDG 379
VTR EVE +++ M+G
Sbjct: 488 VTRTEVEAILREAMEG 535
>gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001A18f, mRNA sequence
Length = 612
Score = 78.5 bits (165), Expect(3) = 2e-021
Identities = 30/64 (46%), Positives = 42/64 (65%)
Frame = +2 / +3
Query: 116 LIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESMWG 295
L+ W PQ+ +L+H A G F THCGWN+TLEAI +GVP P++ADQ + + +
Sbjct: 243 LVRGWAPQVTILSHPAVGGFLTHCGWNATLEAISHGVPALTWPNFADQFCSERLLVDVLR 422
Query: 296 LGVR 307
+GVR
Sbjct: 423 VGVR 434
Score = 30.4 bits (60), Expect(3) = 2e-021
Identities = 13/41 (31%), Positives = 23/41 (56%)
Frame = +2 / +3
Query: 332 VTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGG 454
VTR +VER + ++MDG + R+S + + +A+ G
Sbjct: 489 VTRADVERVVAELMDGRHEGAARRSRAKKLAEEASAVMEDG 611
Score = 30.4 bits (60), Expect(3) = 2e-021
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +2 / +3
Query: 5 ELGNGLYNSGKPFIWVVRSNEEHKLSNELRDK 100
EL GL SG+PF+W ++ + L D+
Sbjct: 111 ELARGLEASGRPFVWAIKEAKADAAVQALLDE 206
>gb|BI957863.1|BI957863 HVSMEn0011M19f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0011M19f, mRNA sequence
Length = 1178
Score = 82.2 bits (173), Expect(2) = 5e-021
Identities = 33/67 (49%), Positives = 41/67 (61%)
Frame = +2 / -3
Query: 110 RGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVPMAAIPHWADQPTISKYMESM 289
RGL+V WC Q+EVLA G F THCGWNS LE++ GVPM P DQ T + +
Sbjct: 609 RGLVVPWCCQVEVLAPPRLGGFLTHCGWNSVLESVWAGVPMLCFPXLTDQFTNRRLVVRE 430
Query: 290 WGLGVRV 310
W +GV +
Sbjct: 429 WRVGVPI 409
Score = 38.2 bits (77), Expect(2) = 5e-021
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = +2 / -3
Query: 320 EKGLVTRDEVERCIKDVMDGDSKDKYRKSATMWMQKAKAAMQNGGSSAKNITEF 481
++G V DEV I+ V+ G+ + R++ K KAA+ GGSS ++ +F
Sbjct: 405 DRGKVFADEVAARIQGVISGEEGQQLRQALKKVRAKLKAAVAPGGSSQRSFDDF 244
>gb|BJ463253.1|BJ463253 BJ463253 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26i01 5', mRNA sequence
Length = 518
Score = 96.8 bits (205), Expect(2) = 6e-021
Identities = 38/92 (41%), Positives = 55/92 (59%)
Frame = +2 / +3
Query: 50 VVRSNEEHKLSNELRDKCKERGLIVSWCPQLEVLAHKATGCFFTHCGWNSTLEAIVNGVP 229
+V+ ++E L E + R ++ +WCPQ +VL +A G F TH GWNS+LE I GVP
Sbjct: 213 LVKGDDEAALPPEFSTATEGRSMLSTWCPQEKVLEQEAVGVFLTHSGWNSSLEGICGGVP 392
Query: 230 MAAIPHWADQPTISKYMESMWGLGVRVRKDEK 325
M P +ADQ T +Y + WG+G+ + D K
Sbjct: 393 MVCWPFFADQQTNCRYKCTEWGIGMEIGDDVK 488
Score = 23.1 bits (44), Expect(2) = 6e-021
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = +2 / +3
Query: 5 ELGNGLYNSGKPFIWVVR 58
E GL N+G F+W VR
Sbjct: 153 EFAWGLANTGYAFLWNVR 206
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 279,456,225
Number of Sequences: 312970
Number of extensions: 4495453
Number of successful extensions: 71086
Number of sequences better than 1.0e-020: 42
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5597587949
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)