TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAT2e04.yg.2.1
         (424 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BU973703.1|BU973703  HB25L04r BC Hordeum vulgare subsp. v...   218   2e-056
gb|BE215908.1|BE215908  HV_CEb0008N01f Hordeum vulgare seedl...   146   7e-035

>gb|BU973703.1|BU973703 HB25L04r BC Hordeum vulgare subsp. vulgare cDNA clone HB25L04
           5-PRIME, mRNA sequence
          Length = 464

 Score =  218 bits (470), Expect = 2e-056
 Identities = 87/116 (75%), Positives = 104/116 (89%)
 Frame = +3 / +2

                                                                       
Query: 3   ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
           ATALP +HPSFSLQQVLEMS++W+++ +P +P++LFIGILSA+NHFAERMA+RKTWMQ  
Sbjct: 116 ATALPMSHPSFSLQQVLEMSDKWRSQPLPRDPVYLFIGILSASNHFAERMAVRKTWMQTS 295

                                                                   
Query: 183 AIQSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQ 350
            I+S   VARFFVAL+ RKE+N  LKKEAEYFGDIVILPFIDRYELVVLKT+AIC+
Sbjct: 296 EIKSSKVVARFFVALNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICE 463




 Score =  130 bits (279), Expect = 3e-030
 Identities = 65/114 (57%), Positives = 73/114 (64%)
 Frame = -1 / -3

                                                                       
Query: 343 IATVFRTTSSYRSMNGRMTMSPKYXXXXXXXXXXXXXXXXATKNRATAFPDWIAGNCIQV 164
           IA V RTTSS RS+NG+MT+SPKY                ATKNRAT   D I+  CIQV
Sbjct: 456 IAIVLRTTSS*RSINGKMTISPKYSASFFSITLTSFLEFSATKNRATTLEDLISEVCIQV 277

                                                                 
Query: 163 FLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHLSDISKTCWREKLGCAFGSAVA 2
           FL AMRSAK F AE +P+KR  GS G+    HLSDIS+TC REKLG   G AVA
Sbjct: 276 FLTAMRSAK*FDAESIPIKR*TGSLGSGWDLHLSDISRTC*REKLGWDIGRAVA 115



>gb|BE215908.1|BE215908 HV_CEb0008N01f Hordeum vulgare seedling green leaf EST library
           HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
           vulgare cDNA clone HV_CEb0008N01f, mRNA sequence
          Length = 520

 Score =  146 bits (313), Expect = 7e-035
 Identities = 57/119 (47%), Positives = 79/119 (66%)
 Frame = +3 / +3

                                                                       
Query: 63  ERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFPAIQSGNAVARFFVALSHRKE 242
           E  KA  VP++ I L +G+ S  N+F  RMA+R+TWMQ+ +++SG    RFF  L    +
Sbjct: 111 ELLKAPPVPKKRIFLLVGVFSTGNNFKRRMALRRTWMQYESVRSGEVAVRFFTGLHKNDQ 290

                                                                      
Query: 243 INAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVHNVTADYIMKCDDDTFVRLD 419
           +N  L +EA+ +GDI  +PF+D Y L+ LKTVAIC +G   + A YIMK DDD FVR+D
Sbjct: 291 VNMELWREAQLYGDIQFMPFVDYYTLITLKTVAICMFGTKIMPAKYIMKTDDDAFVRID 467



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 222,214,424
Number of Sequences: 312970
Number of extensions: 3468595
Number of successful extensions: 139735
Number of sequences better than 1.0e-020: 2
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 3916881059
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)