TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAJ1a04.yg.2.1
         (386 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BE194623.2|BE194623  HVSMEh0086E10f Hordeum vulgare 5-45 ...    77   5e-021

>gb|BE194623.2|BE194623 HVSMEh0086E10f Hordeum vulgare 5-45 DAP spike EST library
           HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
           cDNA clone HVSMEh0086E10f, mRNA sequence
          Length = 819

 Score = 76.7 bits (161), Expect(2) = 5e-021
 Identities = 30/78 (38%), Positives = 39/78 (50%)
 Frame = +3 / +3

                                                                       
Query: 150 YKNFAKXXXXNFXXKXXNWXXFNXPXXFTSFSYGTGVFXPGRCSPGLDWAIPTGNSLXXP 329
           Y +F       F  +  +W  FN P  ++++ Y TGVF PGRCSP +      G+S   P
Sbjct: 171 YVDFTDVCYNEFGDRVKHWTTFNEPWTYSTYGYSTGVFAPGRCSPHVSAFCGAGDSAREP 350

                             
Query: 330 YIGXXNILLAHXXXXXLY 383
           YI   NILL H     LY
Sbjct: 351 YIVTHNILLGHAATVALY 404




 Score = 42.8 bits (87), Expect(2) = 5e-021
 Identities = 16/38 (42%), Positives = 19/38 (50%)
 Frame = +3 / +3

                                                 
Query: 12  YKXXXNXXXXNGIEPYXTLFHWDTPQXLVDXXXXFLDK 125
           Y    N     G++PY TL HWDTP  L D    FL +
Sbjct: 42  YNSLINDVIAKGLKPYVTLHHWDTPLGLEDKYGGFLSE 155



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 96,814,063
Number of Sequences: 312970
Number of extensions: 686582
Number of successful extensions: 37955
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 3400369271
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)