TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF31c07.yg.2.1
(352 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL505646.1|AL505646 AL505646 Hordeum vulgare Barke roots... 73 4e-013
gb|BQ665544.1|BQ665544 HX04B12u HX Hordeum vulgare subsp. v... 66 7e-011
gb|BU974016.1|BU974016 HB26J20r BC Hordeum vulgare subsp. v... 66 7e-011
gb|BF624154.2|BF624154 HVSMEa0012B22f Hordeum vulgare seedl... 55 2e-007
gb|BF624155.2|BF624155 HVSMEa0012B24f Hordeum vulgare seedl... 55 2e-007
gb|AL501628.1|AL501628 AL501628 Hordeum vulgare Barke roots... 34 0.44
gb|AL502910.1|AL502910 AL502910 Hordeum vulgare Barke roots... 34 0.44
gb|BJ458625.1|BJ458625 BJ458625 K. Sato unpublished cDNA li... 34 0.44
gb|BF256571.3|BF256571 HVSMEf0010F23f Hordeum vulgare seedl... 30 4.1
gb|BE214061.3|BE214061 HV_CEb0002B11f Hordeum vulgare seedl... 29 7.7
>gb|AL505646.1|AL505646 AL505646 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09B15V 5', mRNA sequence
Length = 473
Score = 73.5 bits (154), Expect = 4e-013
Identities = 32/61 (52%), Positives = 36/61 (59%)
Frame = +1 / +1
Query: 151 SRIWNETXXAGXFYKPXXYELXXXQXLNMEMXIIMXRDARMDXIXXXXTDXXIXXXXXRE 330
SRIWNET AG FYKP YEL Q LNMEM I+M R++ MD I TD + E
Sbjct: 70 SRIWNETWLAGFFYKPCNYELFVKQSLNMEMAIVMARESGMDWIMHLDTDELLYPGGAPE 249
Query: 331 Y 333
Y
Sbjct: 250 Y 252
Score = 48.7 bits (100), Expect = 1e-005
Identities = 28/61 (45%), Positives = 31/61 (50%)
Frame = -3 / -1
Query: 332 YSRXXXXXXXSSVXXXMXXSILASLXIIXXISMFXXCXXXSS*XXGL*XXPAXQVSFQMR 153
YS SSV + S+ SL I ISM C SS* GL* PA QVSFQ+R
Sbjct: 251 YSGAPPGYSNSSVSRCIIQSMPDSLAITIAISMLSDCLTNSS*LQGL*KNPASQVSFQIR 72
Query: 152 L 150
L
Sbjct: 71 L 69
Score = 35.4 bits (71), Expect = 0.12
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -2 / -3
Query: 276 IHSSIPXHNNXHFHV*XLLXXXFVXXXLVKXTCXXSLIPNAA 151
IH+ P H + H HV LL FV LV+ + +P++A
Sbjct: 195 IHARFPGHYDRHLHVERLLDEQFVVARLVEESREPGFVPDSA 70
Score = 29.9 bits (59), Expect = 5.6
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +3 / +3
Query: 150 QPHLE*DLXSRXXLQAXXLRTXXXAXXKHGN 242
QP+LE +L LQA LRT A +HG+
Sbjct: 69 QPNLERNLARGILLQALQLRTVRQAVAQHGD 161
>gb|BQ665544.1|BQ665544 HX04B12u HX Hordeum vulgare subsp. vulgare cDNA clone HX04B12
3-PRIME, mRNA sequence
Length = 455
Score = 66.1 bits (138), Expect = 7e-011
Identities = 26/39 (66%), Positives = 32/39 (82%)
Frame = +3 / -3
Query: 33 LHQILPWLYYHNVIGVSHFFLLVEGEAAKPAXTSVLESI 149
L+QI+ WLY H V+GV+HF L VEG+A+KP TSVLESI
Sbjct: 432 LYQIMLWLY*HKVVGVAHFLLFVEGKASKPTVTSVLESI 316
Score = 47.8 bits (98), Expect = 2e-005
Identities = 21/39 (53%), Positives = 24/39 (61%)
Frame = -1 / +2
Query: 148 IDSRTEVXAGFAASPSTNRKKCETPMTL**YNHGRIWCR 32
+DSRT V G A PST +KC TP TL * +H IW R
Sbjct: 317 MDSRTLVTVGLEALPSTKSRKCATPTTLC**SHNMIWYR 433
>gb|BU974016.1|BU974016 HB26J20r BC Hordeum vulgare subsp. vulgare cDNA clone HB26J20
5-PRIME, mRNA sequence
Length = 481
Score = 66.1 bits (138), Expect = 7e-011
Identities = 26/39 (66%), Positives = 32/39 (82%)
Frame = +3 / +2
Query: 33 LHQILPWLYYHNVIGVSHFFLLVEGEAAKPAXTSVLESI 149
L+QI+ WLY H V+GV+HF L VEG+A+KP TSVLESI
Sbjct: 176 LYQIMLWLY*HKVVGVAHFLLFVEGKASKPTVTSVLESI 292
Score = 47.8 bits (98), Expect = 2e-005
Identities = 21/39 (53%), Positives = 24/39 (61%)
Frame = -1 / -2
Query: 148 IDSRTEVXAGFAASPSTNRKKCETPMTL**YNHGRIWCR 32
+DSRT V G A PST +KC TP TL * +H IW R
Sbjct: 291 MDSRTLVTVGLEALPSTKSRKCATPTTLC**SHNMIWYR 175
>gb|BF624154.2|BF624154 HVSMEa0012B22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012B22f, mRNA sequence
Length = 488
Score = 54.7 bits (113), Expect = 2e-007
Identities = 24/51 (47%), Positives = 28/51 (54%)
Frame = +1 / +3
Query: 181 GXFYKPXXYELXXXQXLNMEMXIIMXRDARMDXIXXXXTDXXIXXXXXREY 333
G FYKP YEL Q LNMEM I+M R++ MD I TD + EY
Sbjct: 3 GFFYKPCNYELFVKQSLNMEMAIVMARESGMDWIMHLDTDELLYPGGAPEY 155
Score = 31.3 bits (62), Expect = 2.2
Identities = 20/51 (39%), Positives = 22/51 (43%)
Frame = -3 / -2
Query: 332 YSRXXXXXXXSSVXXXMXXSILASLXIIXXISMFXXCXXXSS*XXGL*XXP 180
YS SSV + S+ SL I ISM C SS* GL* P
Sbjct: 154 YSGAPPGYSNSSVSRCIIQSMPDSLAITIAISMLSDCLTNSS*LQGL*KNP 2
Score = 30.9 bits (61), Expect = 3.0
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -2 / -1
Query: 276 IHSSIPXHNNXHFHV*XLLXXXFVXXXLVK 187
IH+ P H + H HV LL FV LV+
Sbjct: 98 IHARFPGHYDRHLHVERLLDEQFVVARLVE 9
>gb|BF624155.2|BF624155 HVSMEa0012B24f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012B24f, mRNA sequence
Length = 491
Score = 54.7 bits (113), Expect = 2e-007
Identities = 24/51 (47%), Positives = 28/51 (54%)
Frame = +1 / +3
Query: 181 GXFYKPXXYELXXXQXLNMEMXIIMXRDARMDXIXXXXTDXXIXXXXXREY 333
G FYKP YEL Q LNMEM I+M R++ MD I TD + EY
Sbjct: 3 GFFYKPCNYELFVKQSLNMEMAIVMARESGMDWIMHLDTDELLYPGGAPEY 155
Score = 31.3 bits (62), Expect = 2.2
Identities = 20/51 (39%), Positives = 22/51 (43%)
Frame = -3 / -2
Query: 332 YSRXXXXXXXSSVXXXMXXSILASLXIIXXISMFXXCXXXSS*XXGL*XXP 180
YS SSV + S+ SL I ISM C SS* GL* P
Sbjct: 154 YSGAPPGYSNSSVSRCIIQSMPDSLAITIAISMLSDCLTNSS*LQGL*KNP 2
Score = 30.9 bits (61), Expect = 3.0
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -2 / -1
Query: 276 IHSSIPXHNNXHFHV*XLLXXXFVXXXLVK 187
IH+ P H + H HV LL FV LV+
Sbjct: 98 IHARFPGHYDRHLHVERLLDEQFVVARLVE 9
>gb|AL501628.1|AL501628 AL501628 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04N12u 3', mRNA sequence
Length = 557
Score = 33.6 bits (67), Expect = 0.44
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3 / -2
Query: 33 LHQILPWLYYHNVIGVSHFFLLVEG 107
L + LPW+ YH V H F+LV+G
Sbjct: 412 LSKKLPWIQYHEVQEGGHMFMLVDG 338
>gb|AL502910.1|AL502910 AL502910 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09C03u 3', mRNA sequence
Length = 577
Score = 33.6 bits (67), Expect = 0.44
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3 / -1
Query: 33 LHQILPWLYYHNVIGVSHFFLLVEG 107
L + LPW+ YH V H F+LV+G
Sbjct: 412 LSKKLPWIQYHEVQEGGHMFMLVDG 338
>gb|BJ458625.1|BJ458625 BJ458625 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak32m09 3', mRNA sequence
Length = 634
Score = 33.6 bits (67), Expect = 0.44
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3 / -1
Query: 33 LHQILPWLYYHNVIGVSHFFLLVEG 107
L + LPW+ YH V H F+LV+G
Sbjct: 373 LSKKLPWIQYHEVQEGGHMFMLVDG 299
>gb|BF256571.3|BF256571 HVSMEf0010F23f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0010F23f, mRNA sequence
Length = 846
Score = 30.4 bits (60), Expect = 4.1
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +2 / -2
Query: 38 PDSSMVVLSQCHWC 79
P +S+V L QCHWC
Sbjct: 653 PSASLVGLCQCHWC 612
>gb|BE214061.3|BE214061 HV_CEb0002B11f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0002B11f, mRNA sequence
Length = 929
Score = 29.5 bits (58), Expect = 7.7
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = +2 / +3
Query: 11 HGKHICWPTPDSSMVVLSQCHW 76
H +H P PD S LS C W
Sbjct: 309 HSQHTYGPPPDFSDTTLSSCRW 374
Database: Hordeum_nucl_with_EST.fasta
Posted date: May 2, 2006 2:38 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 99,123,078
Number of Sequences: 312970
Number of extensions: 916570
Number of successful extensions: 61386
Number of sequences better than 10.0: 14
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 2883857483
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)