TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF18e02.yg.3.1
(315 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU989082.1|BU989082 HF19L09r HF Hordeum vulgare subsp. v... 150 1e-039
gb|BE437521.1|BE437521 SFR004.F04F990621 ITEC SFR Barley Le... 122 5e-028
gb|BU974965.1|BU974965 HB29K23r BC Hordeum vulgare subsp. v... 95 5e-028
gb|AV836624.1|AV836624 AV836624 K. Sato unpublished cDNA li... 32 9e-005
gb|AY943294.1| Hordeum vulgare subsp. vulgare clone BAC 673... 32 1.3
gb|BJ451277.1|BJ451277 BJ451277 K. Sato unpublished cDNA li... 31 2.5
gb|BJ458775.1|BJ458775 BJ458775 K. Sato unpublished cDNA li... 31 2.5
gb|AV929327.1|AV929327 AV929327 K. Sato unpublished cDNA li... 26 2.9
gb|AV937130.1|AV937130 AV937130 K. Sato unpublished cDNA li... 26 3.0
gb|BJ474819.1|BJ474819 BJ474819 K. Sato unpublished cDNA li... 26 3.2
gb|BJ477407.1|BJ477407 BJ477407 K. Sato unpublished cDNA li... 22 3.3
gb|BQ466025.1|BQ466025 HT01F20T HT Hordeum vulgare subsp. v... 30 3.4
gb|AF427791.1| Hordeum vulgare Mla locus, complete sequence 30 3.4
gb|AV935408.1|AV935408 AV935408 K. Sato unpublished cDNA li... 26 3.9
gb|BJ474370.1|BJ474370 BJ474370 K. Sato unpublished cDNA li... 22 4.2
gb|BJ469163.1|BJ469163 BJ469163 K. Sato unpublished cDNA li... 22 5.4
gb|BJ471887.1|BJ471887 BJ471887 K. Sato unpublished cDNA li... 22 5.4
gb|BJ473228.1|BJ473228 BJ473228 K. Sato unpublished cDNA li... 22 5.7
gb|BQ471000.1|BQ471000 HX04K20r HX Hordeum vulgare subsp. v... 24 6.6
gb|CA030427.1|CA030427 HX07A15r HX Hordeum vulgare subsp. v... 24 7.1
gb|CA030735.1|CA030735 HX07P10r HX Hordeum vulgare subsp. v... 24 8.2
gb|CB875019.1|CB875019 HX07A15w HX Hordeum vulgare subsp. v... 24 8.5
gb|CB875325.1|CB875325 HX07P10w HX Hordeum vulgare subsp. v... 24 8.5
>gb|BU989082.1|BU989082 HF19L09r HF Hordeum vulgare subsp. vulgare cDNA clone HF19L09
5-PRIME, mRNA sequence
Length = 614
Score = 150 bits (322), Expect(2) = 1e-039
Identities = 62/85 (72%), Positives = 71/85 (83%)
Frame = +3 / +1
Query: 60 HISYFSIDSYAKLVVMVLKYSSVEITPNKGSILSKIISVTVRTIQKDAEEKKASFNPRPY 239
HISYF IDSY+KLV VLK SVEI PNKGS+L KI+ +T+R IQK+AEEKKASFNPRPY
Sbjct: 157 HISYFPIDSYSKLVSAVLKNCSVEIGPNKGSLLPKILLLTIRIIQKEAEEKKASFNPRPY 336
Query: 240 FRLXINWLYDLTTTDGHHDGSNFQV 314
FRL INWL DL T++ HHDG+N QV
Sbjct: 337 FRLFINWLNDLITSEPHHDGTNLQV 411
Score = 109 bits (233), Expect(2) = 1e-023
Identities = 48/85 (56%), Positives = 61/85 (71%)
Frame = -2 / -3
Query: 314 NLKIRTIMMAISSSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFIWC 135
NL+I +IMM +KVI+PIY Q++VWSG+K*SFL S FL+ P+ NLGK AF+W
Sbjct: 411 NLQISSIMMRFRCNKVIQPIYKQTKVWSGVK*SFLLFSFFLDDPNCQ*QNLGKKAAFVWS 232
Query: 134 YLH*RVLENHNHELCI*INGKI*NM 60
Y H* VL + H+L I*ING+I* M
Sbjct: 231 YFH*TVL*HRRHKLRI*INGEI*YM 157
Score = 104 bits (222), Expect = 2e-022
Identities = 52/84 (61%), Positives = 57/84 (67%)
Frame = -3 / -1
Query: 313 T*KLEPS*WPXXXXXXXSQFIXNRKYGRGLNEAFFSSASF*MVLTVTEIILESILPLFGV 134
T KL PS* SQFI RKYGRGLNEAFFSSASF M+L V+ IL LPLFG
Sbjct: 410 TCKLVPS**GSDVIRSFSQFINRRKYGRGLNEAFFSSASFWMILIVSNRILGRRLPLFGP 231
Query: 133 ISTEEYLRTITTSFAYESMEKYEI 62
ISTE++ T TSF YESM KY+I
Sbjct: 230 ISTEQFFSTADTSFEYESMGKYDI 159
Score = 91.3 bits (193), Expect(2) = 2e-020
Identities = 50/90 (55%), Positives = 58/90 (64%)
Frame = +2 / +3
Query: 44 ITTATPYFIFFH*FICKARGYGSQVLFSGDNTK*RQYTFQDYFCYCEDHSERC*GKESFI 223
+ T Y IF H*FI +A G++ LFSG+ TK RQ + QD DH ER * +ESFI
Sbjct: 141 VPTVITYIIFPH*FIFEACVCGAKELFSGNRTKQRQPSSQDSVTDN*DHPERS*REESFI 320
Query: 224 *SPTILPIVYKLAL*PYYY*WPS*WF*FSG 313
* PTIL VYKLA *PYY * S*W *F+G
Sbjct: 321 *PPTILSSVYKLAE*PYYI*TSS*WN*FAG 410
Score = 48.7 bits (100), Expect(2) = 3e-012
Identities = 28/41 (68%), Positives = 28/41 (68%)
Frame = -1 / -2
Query: 315 KPEN*NHHDGHQ***GHRANLXTIGSMVGD*MKLSFPQHLS 193
KP N* HHD Q **GH ANL T SMVG *MKLS Q LS
Sbjct: 412 KPAN*FHHDEVQM**GHSANL*TDESMVGG*MKLSSLQLLS 290
Score = 42.8 bits (87), Expect(2) = 4e-010
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = +1 / +2
Query: 49 HSNPIFHIFPLIHMQSSWLWFSSTLQWR*HQIKAVY 156
HS+ I+HI PLIH++S L T+QW+* Q KA +
Sbjct: 146 HSHHIYHISPLIHIRSLCLRC*RTVQWK*DQTKAAF 253
Score = 39.6 bits (80), Expect(2) = 3e-012
Identities = 18/34 (52%), Positives = 22/34 (64%)
Frame = -1 / -2
Query: 150 CLYLVLSPLKST*EP*PRALHMNQWKNMKYGVAV 49
CL LVL PL S+ P +A +MNQW NM Y + V
Sbjct: 247 CLCLVLFPLNSSLAPQTQASNMNQWGNMIYVMTV 146
Score = 38.2 bits (77), Expect(2) = 4e-010
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = +1 / +2
Query: 196 KMLRKRKLHLIPDHTSDCX*IG 261
K L++RKLHL PDHT C *IG
Sbjct: 293 KKLKRRKLHLTPDHTFVCL*IG 358
Score = 30.4 bits (60), Expect(2) = 1e-039
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / +3
Query: 2 SLRANCCSWWIISAITT 52
SL AN CSWWI+ + T
Sbjct: 108 SL*ANYCSWWIVPTVIT 158
Score = 24.4 bits (47), Expect(2) = 2e-020
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +1 / +2
Query: 1 *SPSKLLLLVDHLSNHH 51
*S SKLLLLVD +HH
Sbjct: 107 *SLSKLLLLVDCPHSHH 157
Score = 17.1 bits (31), Expect(2) = 1e-023
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = -3 / -1
Query: 22 ATICSET 2
A ICSET
Sbjct: 128 AVICSET 108
>gb|BE437521.1|BE437521 SFR004.F04F990621 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 839
Score = 122 bits (262), Expect = 5e-028
Identities = 50/69 (72%), Positives = 58/69 (84%)
Frame = +3 / +2
Query: 108 VLKYSSVEITPNKGSILSKIISVTVRTIQKDAEEKKASFNPRPYFRLXINWLYDLTTTDG 287
VLK SVEI PNKGS+L KI+ +T+R IQK+AEEKKASFNPRPYFRL INWL DL T++
Sbjct: 2 VLKNCSVEIGPNKGSLLPKILLLTIRIIQKEAEEKKASFNPRPYFRLFINWLNDLITSEP 181
Query: 288 HHDGSNFQV 314
HHDG+N QV
Sbjct: 182 HHDGTNLQV 208
Score = 92.7 bits (196), Expect = 6e-019
Identities = 38/67 (56%), Positives = 48/67 (71%)
Frame = -2 / -2
Query: 314 NLKIRTIMMAISSSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFIWC 135
NL+I +IMM +KVI+PIY Q++VWSG+K*SFL S FL+ P+ NLGK AF+W
Sbjct: 208 NLQISSIMMRFRCNKVIQPIYKQTKVWSGVK*SFLLFSFFLDDPNCQ*QNLGKKAAFVWS 29
Query: 134 YLH*RVL 114
Y H* VL
Sbjct: 28 YFH*TVL 8
Score = 82.2 bits (173), Expect = 9e-016
Identities = 42/69 (60%), Positives = 46/69 (66%)
Frame = -3 / -3
Query: 313 T*KLEPS*WPXXXXXXXSQFIXNRKYGRGLNEAFFSSASF*MVLTVTEIILESILPLFGV 134
T KL PS* SQFI RKYGRGLNEAFFSSASF M+L V+ IL LPLFG
Sbjct: 207 TCKLVPS**GSDVIRSFSQFINRRKYGRGLNEAFFSSASFWMILIVSNRILGRRLPLFGP 28
Query: 133 ISTEEYLRT 107
ISTE++ T
Sbjct: 27 ISTEQFFST 1
Score = 76.7 bits (161), Expect = 4e-014
Identities = 41/69 (59%), Positives = 47/69 (68%)
Frame = +2 / +1
Query: 107 GSQVLFSGDNTK*RQYTFQDYFCYCEDHSERC*GKESFI*SPTILPIVYKLAL*PYYY*W 286
G++ LFSG+ TK RQ + QD DH ER * +ESFI* PTIL VYKLA *PYY *
Sbjct: 1 GAKELFSGNRTKQRQPSSQDSVTDN*DHPERS*REESFI*PPTILSSVYKLAE*PYYI*T 180
Query: 287 PS*WF*FSG 313
S*W *F+G
Sbjct: 181 SS*WN*FAG 207
Score = 48.7 bits (100), Expect = 1e-005
Identities = 28/41 (68%), Positives = 28/41 (68%)
Frame = -1 / -1
Query: 315 KPEN*NHHDGHQ***GHRANLXTIGSMVGD*MKLSFPQHLS 193
KP N* HHD Q **GH ANL T SMVG *MKLS Q LS
Sbjct: 209 KPAN*FHHDEVQM**GHSANL*TDESMVGG*MKLSSLQLLS 87
Score = 38.2 bits (77), Expect = 0.016
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = +1 / +3
Query: 196 KMLRKRKLHLIPDHTSDCX*IG 261
K L++RKLHL PDHT C *IG
Sbjct: 90 KKLKRRKLHLTPDHTFVCL*IG 155
>gb|BU974965.1|BU974965 HB29K23r BC Hordeum vulgare subsp. vulgare cDNA clone HB29K23
5-PRIME, mRNA sequence
Length = 611
Score = 94.5 bits (200), Expect(3) = 5e-028
Identities = 39/59 (66%), Positives = 46/59 (77%)
Frame = +3 / +3
Query: 126 VEITPNKGSILSKIISVTVRTIQKDAEEKKASFNPRPYFRLXINWLYDLTTTDGHHDGS 302
V++ PNKGS+L KI+S+T R IQKDAEEKK SFNPRPYFRL IN L +L+T D H S
Sbjct: 123 VDLGPNKGSLLPKILSITTRIIQKDAEEKKVSFNPRPYFRLFINLLSELSTADLHDGAS 299
Score = 63.8 bits (133), Expect(3) = 4e-019
Identities = 30/45 (66%), Positives = 32/45 (71%)
Frame = -3 / -2
Query: 259 QFIXNRKYGRGLNEAFFSSASF*MVLTVTEIILESILPLFGVIST 125
+FI N KYGRGL E FFSSASF*M+L V E I LPLFG ST
Sbjct: 256 KFINNLKYGRGLKETFFSSASF*MILVVMERIFGRRLPLFGPKST 122
Score = 57.9 bits (120), Expect(2) = 3e-013
Identities = 24/56 (42%), Positives = 35/56 (62%)
Frame = -2 / -1
Query: 293 MMAISSSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFIWCYLH 126
+M +SS+K+ IY S++W ++ +FLF SIFLN P NL K AF+W +H
Sbjct: 290 IMKVSSAKLT*QIYK*SKIWPRVERNFLFFSIFLNDPSCYGENLRKKAAFVWS*IH 123
Score = 43.2 bits (88), Expect(3) = 5e-028
Identities = 19/37 (51%), Positives = 23/37 (62%)
Frame = +2 / +2
Query: 8 RANCCSWWIISAITTATPYFIFFH*FICKARGYGSQV 118
RA+ SWW +SA+ TP FIF *FI KA + QV
Sbjct: 8 RASYRSWWDVSAVGPTTPDFIFLS*FILKACDFNVQV 118
Score = 40.9 bits (83), Expect(3) = 5e-011
Identities = 22/48 (45%), Positives = 27/48 (56%)
Frame = +2 / +2
Query: 119 LFSGDNTK*RQYTFQDYFCYCEDHSERC*GKESFI*SPTILPIVYKLA 262
++ G TK RQ + +D DHSERC* KESF I I+YK A
Sbjct: 116 VWRGFRTKQRQPSSEDSLHNN*DHSERC*RKESFFQPSAIF*IIYKFA 259
Score = 39.1 bits (79), Expect(3) = 5e-011
Identities = 15/36 (41%), Positives = 21/36 (58%)
Frame = +3 / +3
Query: 12 QIVAXXXXXXXXXXXXHISYFSIDSYAKLVVMVLKY 119
Q++A ISYFS+DSY+KLV ++ KY
Sbjct: 12 QVIAPGGMSQQSAQQLQISYFSVDSYSKLVTLMFKY 119
Score = 36.3 bits (73), Expect(3) = 4e-019
Identities = 14/20 (70%), Positives = 14/20 (70%)
Frame = -3 / -2
Query: 118 YLRTITTSFAYESMEKYEIW 59
YL TSF YES EKYEIW
Sbjct: 118 YLNIKVTSFEYESTEKYEIW 59
Score = 34.5 bits (69), Expect(2) = 0.004
Identities = 20/36 (55%), Positives = 21/36 (58%)
Frame = -1 / -3
Query: 297 HHDGHQ***GHRANLXTIGSMVGD*MKLSFPQHLSE 190
HH Q * H ANL I +M * KLSF QHLSE
Sbjct: 294 HHHEGQQC*AHLANL*II*NMAEG*KKLSFLQHLSE 187
Score = 33.6 bits (67), Expect(2) = 3e-013
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -1 / -3
Query: 99 RALHMNQWKNMKYGVAVVIAEMIHQEQ 19
+AL MNQ +NMK GV AE HQE+
Sbjct: 99 QALSMNQLRNMKSGVVGPTAETSHQER 19
Score = 32.2 bits (64), Expect(2) = 0.003
Identities = 16/28 (57%), Positives = 18/28 (64%)
Frame = +1 / +1
Query: 13 KLLLLVDHLSNHHSNPIFHIFPLIHMQS 96
KL LLV LS+ +N FHI LIH QS
Sbjct: 13 KLSLLVGCLSSRPNNSRFHISQLIHTQS 96
Score = 27.6 bits (54), Expect(3) = 4e-019
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = -1 / -3
Query: 315 KPEN*NHHDGHQ 280
KPEN*+HH+G Q
Sbjct: 309 KPEN*HHHEGQQ 274
Score = 25.4 bits (49), Expect(2) = 0.003
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = +1 / +1
Query: 115 STLQWR*HQIKAVYXXXXXXXXXXXXXKMLRKRKLHLIPDHTSDCX*I 258
S++ W * Q KA + KML+KRK H D *I
Sbjct: 112 SSMAWI*DQTKAAFFRRFSP*QLGSFRKMLKKRKFLSTLGHILDYL*I 255
Score = 22.6 bits (43), Expect(2) = 0.004
Identities = 10/20 (50%), Positives = 17/20 (85%)
Frame = -2 / -1
Query: 119 VLENHNHELCI*INGKI*NM 60
+LE+ +H+L +*IN +I*N+
Sbjct: 119 ILEH*SHKL*V*IN*EI*NL 60
Score = 20.3 bits (38), Expect(3) = 5e-028
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +2 / +2
Query: 287 PS*WF*FSG 313
PS*W *FSG
Sbjct: 281 PS*WC*FSG 307
Score = 19.9 bits (37), Expect(3) = 5e-011
Identities = 6/8 (75%), Positives = 8/8 (100%)
Frame = +3 / +3
Query: 291 HDGSNFQV 314
HDG++FQV
Sbjct: 285 HDGASFQV 308
>gb|AV836624.1|AV836624 AV836624 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19c09, mRNA
sequence
Length = 620
Score = 32.2 bits (64), Expect(2) = 9e-005
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = +3 / +3
Query: 60 HISYFSIDSYAKLVVMV 110
HISYF IDSY+KLV V
Sbjct: 570 HISYFPIDSYSKLVSAV 620
Score = 30.4 bits (60), Expect(2) = 9e-005
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / +2
Query: 2 SLRANCCSWWIISAITT 52
SL AN CSWWI+ + T
Sbjct: 521 SL*ANYCSWWIVPTVIT 571
Score = 29.0 bits (57), Expect(2) = 0.040
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = +1 / +1
Query: 49 HSNPIFHIFPLIHMQS 96
HS+ I+HI PLIH++S
Sbjct: 559 HSHHIYHISPLIHIRS 606
Score = 24.4 bits (47), Expect(2) = 0.040
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +1 / +1
Query: 1 *SPSKLLLLVDHLSNHH 51
*S SKLLLLVD +HH
Sbjct: 520 *SLSKLLLLVDCPHSHH 570
>gb|AY943294.1| Hordeum vulgare subsp. vulgare clone BAC 673I14, complete sequence
Length = 108608
Score = 31.8 bits (63), Expect = 1.3
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +3 / -1
Query: 78 IDSYAKLVVMVLKYSSVEITPNKGSILSKIISVTVRTIQKDAEEKKASFNP 230
ID +A+LV +V Y + + S+ K + ++ ++KD+ E+ FNP
Sbjct: 15323 IDMWAQLVKVVFLYPGTRLKSHF*SLSKKQEGLFLKRLEKDSIERTFFFNP 15171
>gb|BJ451277.1|BJ451277 BJ451277 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak33g07 5', mRNA sequence
Length = 609
Score = 30.9 bits (61), Expect = 2.5
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3 / -3
Query: 243 RLXINWLYDLTTTDGHHDGSNFQ 311
R+ INW+ DL DGH+ F+
Sbjct: 523 RISINWIEDLGNADGHNKNFTFK 455
>gb|BJ458775.1|BJ458775 BJ458775 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak33g07 3', mRNA sequence
Length = 555
Score = 30.9 bits (61), Expect = 2.5
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3 / +1
Query: 243 RLXINWLYDLTTTDGHHDGSNFQ 311
R+ INW+ DL DGH+ F+
Sbjct: 328 RISINWIEDLGNADGHNKNFTFK 396
>gb|AV929327.1|AV929327 AV929327 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd11n02 3', mRNA sequence
Length = 496
Score = 26.3 bits (51), Expect(2) = 2.9
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1 / -2
Query: 37 LSNHHSNPIFHIFPLIHMQSSWL 105
LSN H + HI P+IH+ + +L
Sbjct: 378 LSNGHGSFCIHIPPVIHLTAQFL 310
Score = 20.3 bits (38), Expect(2) = 2.9
Identities = 5/7 (71%), Positives = 5/7 (71%)
Frame = +2 / -2
Query: 14 NCCSWWI 34
NC WWI
Sbjct: 486 NCTIWWI 466
>gb|AV937130.1|AV937130 AV937130 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal9f18 3', mRNA sequence
Length = 512
Score = 26.3 bits (51), Expect(2) = 3.0
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1 / -3
Query: 37 LSNHHSNPIFHIFPLIHMQSSWL 105
LSN H + HI P+IH+ + +L
Sbjct: 360 LSNGHGSFCIHIPPVIHLTAQFL 292
Score = 20.3 bits (38), Expect(2) = 3.0
Identities = 5/7 (71%), Positives = 5/7 (71%)
Frame = +2 / -3
Query: 14 NCCSWWI 34
NC WWI
Sbjct: 468 NCTIWWI 448
>gb|BJ474819.1|BJ474819 BJ474819 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal22j14 3', mRNA sequence
Length = 550
Score = 26.3 bits (51), Expect(2) = 3.2
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1 / -2
Query: 37 LSNHHSNPIFHIFPLIHMQSSWL 105
LSN H + HI P+IH+ + +L
Sbjct: 369 LSNGHGSFCIHIPPVIHLTAQFL 301
Score = 20.3 bits (38), Expect(2) = 3.2
Identities = 5/7 (71%), Positives = 5/7 (71%)
Frame = +2 / -2
Query: 14 NCCSWWI 34
NC WWI
Sbjct: 477 NCTIWWI 457
>gb|BJ477407.1|BJ477407 BJ477407 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal41d09 3', mRNA sequence
Length = 318
Score = 21.7 bits (41), Expect(3) = 3.3
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -2 / -1
Query: 296 IMMAISSSKVIEPIYXQSEVW 234
++ A++SSK P+Y + VW
Sbjct: 246 MIAALASSKDGWPLYAAAAVW 184
Score = 20.8 bits (39), Expect(3) = 3.3
Identities = 6/8 (75%), Positives = 7/8 (87%)
Frame = -1 / -3
Query: 99 RALHMNQW 76
RALH+ QW
Sbjct: 136 RALHLRQW 113
Score = 16.6 bits (30), Expect(3) = 3.3
Identities = 4/6 (66%), Positives = 5/6 (83%)
Frame = -1 / -3
Query: 300 NHHDGH 283
+HH GH
Sbjct: 280 HHHHGH 263
>gb|BQ466025.1|BQ466025 HT01F20T HT Hordeum vulgare subsp. vulgare cDNA clone HT01F20
5-PRIME, mRNA sequence
Length = 395
Score = 30.4 bits (60), Expect = 3.4
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +1 / -1
Query: 7 PSKLLLLVDHLSNHHSNPIFHIFPLIHMQSSW 102
P+ LL L H+ HH NP+ IH +S W
Sbjct: 386 PALLL*LSQHMVWHHGNPMKQWLGPIHRRSIW 291
>gb|AF427791.1| Hordeum vulgare Mla locus, complete sequence
Length = 261265
Score = 30.4 bits (60), Expect = 3.4
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = -3 / -1
Query: 214 FFSSASF*MVLTVTEIILESILPLFGVISTEEYLRTITTSFAYES 80
FFSS+ L+ + L+ IL +I EYLRT + + +
Sbjct: 70540 FFSSSMLLSALSFRGVELQGILAHIAIIQISEYLRTKDVRYCFNT 70406
Score = 30.4 bits (60), Expect = 3.4
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = -3 / -3
Query: 214 FFSSASF*MVLTVTEIILESILPLFGVISTEEYLRTITTSFAYES 80
FFSS+ L+ + L+ IL +I EYLRT + + +
Sbjct: 110255 FFSSSMLLSALSFRGVELQGILAHIAIIQISEYLRTKDVRYCFNT 110121
>gb|AV935408.1|AV935408 AV935408 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal4a13 3', mRNA sequence
Length = 687
Score = 26.3 bits (51), Expect(2) = 3.9
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +1 / -3
Query: 37 LSNHHSNPIFHIFPLIHMQSSWL 105
LSN H + HI P+IH+ + +L
Sbjct: 361 LSNGHGSFCIHIPPVIHLTAQFL 293
Score = 20.3 bits (38), Expect(2) = 3.9
Identities = 5/7 (71%), Positives = 5/7 (71%)
Frame = +2 / -3
Query: 14 NCCSWWI 34
NC WWI
Sbjct: 469 NCTIWWI 449
>gb|BJ474370.1|BJ474370 BJ474370 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal13h12 3', mRNA sequence
Length = 364
Score = 21.7 bits (41), Expect(3) = 4.2
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -2 / -3
Query: 296 IMMAISSSKVIEPIYXQSEVW 234
++ A++SSK P+Y + VW
Sbjct: 248 MIAALASSKDGWPLYAAAAVW 186
Score = 20.8 bits (39), Expect(3) = 4.2
Identities = 6/8 (75%), Positives = 7/8 (87%)
Frame = -1 / -2
Query: 99 RALHMNQW 76
RALH+ QW
Sbjct: 138 RALHLRQW 115
Score = 16.6 bits (30), Expect(3) = 4.2
Identities = 4/6 (66%), Positives = 5/6 (83%)
Frame = -1 / -2
Query: 300 NHHDGH 283
+HH GH
Sbjct: 282 HHHHGH 265
>gb|BJ469163.1|BJ469163 BJ469163 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal13h12 5', mRNA sequence
Length = 414
Score = 21.7 bits (41), Expect(3) = 5.4
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -2 / +3
Query: 296 IMMAISSSKVIEPIYXQSEVW 234
++ A++SSK P+Y + VW
Sbjct: 117 MIAALASSKDGWPLYAAAAVW 179
Score = 20.8 bits (39), Expect(3) = 5.4
Identities = 6/8 (75%), Positives = 7/8 (87%)
Frame = -1 / +2
Query: 99 RALHMNQW 76
RALH+ QW
Sbjct: 227 RALHLRQW 250
Score = 16.6 bits (30), Expect(3) = 5.4
Identities = 4/6 (66%), Positives = 5/6 (83%)
Frame = -1 / +2
Query: 300 NHHDGH 283
+HH GH
Sbjct: 83 HHHHGH 100
>gb|BJ471887.1|BJ471887 BJ471887 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal28e19 5', mRNA sequence
Length = 414
Score = 21.7 bits (41), Expect(3) = 5.4
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -2 / +3
Query: 296 IMMAISSSKVIEPIYXQSEVW 234
++ A++SSK P+Y + VW
Sbjct: 117 MIAALASSKDGWPLYAAAAVW 179
Score = 20.8 bits (39), Expect(3) = 5.4
Identities = 6/8 (75%), Positives = 7/8 (87%)
Frame = -1 / +2
Query: 99 RALHMNQW 76
RALH+ QW
Sbjct: 227 RALHLRQW 250
Score = 16.6 bits (30), Expect(3) = 5.4
Identities = 4/6 (66%), Positives = 5/6 (83%)
Frame = -1 / +2
Query: 300 NHHDGH 283
+HH GH
Sbjct: 83 HHHHGH 100
>gb|BJ473228.1|BJ473228 BJ473228 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal41d09 5', mRNA sequence
Length = 428
Score = 21.7 bits (41), Expect(3) = 5.7
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -2 / +2
Query: 296 IMMAISSSKVIEPIYXQSEVW 234
++ A++SSK P+Y + VW
Sbjct: 131 MIAALASSKDGWPLYAAAAVW 193
Score = 20.8 bits (39), Expect(3) = 5.7
Identities = 6/8 (75%), Positives = 7/8 (87%)
Frame = -1 / +1
Query: 99 RALHMNQW 76
RALH+ QW
Sbjct: 241 RALHLRQW 264
Score = 16.6 bits (30), Expect(3) = 5.7
Identities = 4/6 (66%), Positives = 5/6 (83%)
Frame = -1 / +1
Query: 300 NHHDGH 283
+HH GH
Sbjct: 97 HHHHGH 114
>gb|BQ471000.1|BQ471000 HX04K20r HX Hordeum vulgare subsp. vulgare cDNA clone HX04K20
5-PRIME, mRNA sequence
Length = 473
Score = 24.0 bits (46), Expect(2) = 6.6
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = +2 / +3
Query: 2 SLRANCCSWWIISAITTATP 61
S R+ CSWW ++TP
Sbjct: 156 SRRSPACSWWTPRRSRSSTP 215
Score = 21.2 bits (40), Expect(2) = 6.6
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +3 / +3
Query: 228 PRPYFRLXINWLYD 269
PRP RL W YD
Sbjct: 393 PRPPPRLTSPWTYD 434
>gb|CA030427.1|CA030427 HX07A15r HX Hordeum vulgare subsp. vulgare cDNA clone HX07A15
5-PRIME, mRNA sequence
Length = 504
Score = 24.0 bits (46), Expect(2) = 7.1
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = +2 / +2
Query: 2 SLRANCCSWWIISAITTATP 61
S R+ CSWW ++TP
Sbjct: 143 SRRSPACSWWTPRRSRSSTP 202
Score = 21.2 bits (40), Expect(2) = 7.1
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +3 / +2
Query: 228 PRPYFRLXINWLYD 269
PRP RL W YD
Sbjct: 380 PRPPPRLTSPWTYD 421
>gb|CA030735.1|CA030735 HX07P10r HX Hordeum vulgare subsp. vulgare cDNA clone HX07P10
5-PRIME, mRNA sequence
Length = 588
Score = 24.0 bits (46), Expect(2) = 8.2
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = +2 / +3
Query: 2 SLRANCCSWWIISAITTATP 61
S R+ CSWW ++TP
Sbjct: 150 SRRSPACSWWTPRRSRSSTP 209
Score = 21.2 bits (40), Expect(2) = 8.2
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +3 / +3
Query: 228 PRPYFRLXINWLYD 269
PRP RL W YD
Sbjct: 387 PRPPPRLTSPWTYD 428
>gb|CB875019.1|CB875019 HX07A15w HX Hordeum vulgare subsp. vulgare cDNA clone HX07A15
3-PRIME, mRNA sequence
Length = 613
Score = 24.0 bits (46), Expect(2) = 8.5
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = +2 / -3
Query: 2 SLRANCCSWWIISAITTATP 61
S R+ CSWW ++TP
Sbjct: 611 SRRSPACSWWTPRRSRSSTP 552
Score = 21.2 bits (40), Expect(2) = 8.5
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +3 / -3
Query: 228 PRPYFRLXINWLYD 269
PRP RL W YD
Sbjct: 374 PRPPPRLTSPWTYD 333
>gb|CB875325.1|CB875325 HX07P10w HX Hordeum vulgare subsp. vulgare cDNA clone HX07P10
3-PRIME, mRNA sequence
Length = 613
Score = 24.0 bits (46), Expect(2) = 8.5
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = +2 / -3
Query: 2 SLRANCCSWWIISAITTATP 61
S R+ CSWW ++TP
Sbjct: 611 SRRSPACSWWTPRRSRSSTP 552
Score = 21.2 bits (40), Expect(2) = 8.5
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +3 / -3
Query: 228 PRPYFRLXINWLYD 269
PRP RL W YD
Sbjct: 374 PRPPPRLTSPWTYD 333
Database: Hordeum_nucl_with_EST.fasta
Posted date: May 2, 2006 2:38 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 110,266,831
Number of Sequences: 312970
Number of extensions: 1128627
Number of successful extensions: 84123
Number of sequences better than 10.0: 30
length of database: 58,378,179
effective HSP length: 48
effective length of database: 43,355,619
effective search space used: 2427914664
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)