TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF12e06.yg.2.1
(561 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA li... 83 5e-036
gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. v... 73 9e-034
gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-a... 71 2e-033
gb|AV834161.1|AV834161 AV834161 K. Sato unpublished cDNA li... 138 2e-032
gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green... 80 5e-032
gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA li... 53 1e-030
gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. v... 83 2e-028
gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgar... 88 1e-027
gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA li... 91 3e-027
gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. v... 91 4e-027
gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm librar... 88 1e-023
gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA li... 94 3e-023
gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA li... 94 3e-023
gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-a... 69 4e-023
gb|AV834211.1|AV834211 AV834211 K. Sato unpublished cDNA li... 103 7e-022
gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day ger... 66 3e-021
gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-a... 88 8e-021
>gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 5', mRNA sequence
Length = 598
Score = 83.1 bits (175), Expect(3) = 5e-036
Identities = 31/66 (46%), Positives = 42/66 (63%)
Frame = +3 / +2
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
+L LP GL+ F L +PL+ SW GLG++ I +I+ AA HYNG MKPWL+L +
Sbjct: 398 KLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMT 577
Query: 519 DYKNYW 536
Y+ YW
Sbjct: 578 KYRPYW 595
Score = 68.9 bits (144), Expect(3) = 5e-036
Identities = 26/64 (40%), Positives = 41/64 (64%)
Frame = +3 / +2
Query: 3 ISIFGLSHFLLPDLLPSLNRVVVLDDDLIVQKDLSPLWNLDMGGKVIGAVQFCGVRLGQL 182
+S+ F LP + P L++++ LDDD++VQKDL+ LW++D+ G V GAV CG +
Sbjct: 50 LSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNGAVFTCGESFHRF 229
Query: 183 KPYI 194
Y+
Sbjct: 230 DKYL 241
Score = 36.8 bits (74), Expect(3) = 5e-036
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = +3 / +2
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D ++C W G+N+ +L +W++ IT ++
Sbjct: 269 NFDPNACGWAYGMNIFDLKQWKNKDITGIY 358
>gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10L09
5-PRIME, mRNA sequence
Length = 684
Score = 73.0 bits (153), Expect(3) = 9e-034
Identities = 27/64 (42%), Positives = 41/64 (64%)
Frame = +3 / +3
Query: 3 ISIFGLSHFLLPDLLPSLNRVVVLDDDLIVQKDLSPLWNLDMGGKVIGAVQFCGVRLGQL 182
+S+ F LP + P LN+++ LDDD++VQKDL+ LW +D+ G V GAV+ CG +
Sbjct: 174 LSMLNHLRFYLPQVYPKLNKILFLDDDIVVQKDLTGLWEVDLNGNVNGAVETCGESFHRF 353
Query: 183 KPYI 194
Y+
Sbjct: 354 DKYL 365
Score = 71.6 bits (150), Expect(3) = 9e-034
Identities = 26/54 (48%), Positives = 36/54 (66%)
Frame = +3 / +3
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPW 500
+L LP GL+ F L +PL+ SW GLG++ + H +I+ AA +HYNG MKPW
Sbjct: 522 KLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPTVEHAEIDTAAVIHYNGNMKPW 683
Score = 36.3 bits (73), Expect(3) = 9e-034
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = +3 / +3
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D ++C W G+N+ +L++W+ IT ++
Sbjct: 393 NFDPNACGWAYGMNMFDLEEWKKKDITGIY 482
>gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006H13f, mRNA sequence
Length = 1018
Score = 70.7 bits (148), Expect(3) = 2e-033
Identities = 26/63 (41%), Positives = 42/63 (66%)
Frame = +3 / +1
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
+L LP GL+ F + +PL+ SW GLG++ +S D+E+AA +HY G +KPWL+ G+
Sbjct: 484 KLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYXGHLKPWLEXGLP 663
Query: 519 DYK 527
++
Sbjct: 664 SFE 672
Score = 68.9 bits (144), Expect(3) = 2e-033
Identities = 26/64 (40%), Positives = 43/64 (67%)
Frame = +3 / +1
Query: 3 ISIFGLSHFLLPDLLPSLNRVVVLDDDLIVQKDLSPLWNLDMGGKVIGAVQFCGVRLGQL 182
+S+ F LP++ P L+++V LD D++V+KD++ LW++DM GKV GAV+ CG +
Sbjct: 136 LSMLNHLRFYLPEIYPKLDKMVFLDGDVVVRKDITGLWSIDMKGKVNGAVETCGESFHRF 315
Query: 183 KPYI 194
Y+
Sbjct: 316 GRYL 327
Score = 40.5 bits (82), Expect(3) = 2e-033
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3 / +1
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D D+C W G+NV +L +WR IT L+
Sbjct: 355 NFDPDACGWAFGMNVFDLAEWRRQNITELY 444
>gb|AV834161.1|AV834161 AV834161 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags5h22, mRNA sequence
Length = 714
Score = 138 bits (297), Expect = 2e-032
Identities = 56/113 (49%), Positives = 75/113 (66%)
Frame = +3 / -1
Query: 210 DDDSCVWLSGLNVIELDKWRDTGITSLHDQSVQKLRKDSLKSQRLQALPAGLLAFQDLVY 389
D SC W+SG+NVI LDKWR +T + +++++K S R A LL+FQ+L+Y
Sbjct: 702 DPKSCAWMSGVNVINLDKWRKHKVTENYLLLLKQVKKKDEVSLREAAFSLSLLSFQNLIY 523
Query: 390 PLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGILDYKNYWRKYL 548
PL+ SGLG+DYGI + +A LHYNG MKPWL+LGI DYK YWR++L
Sbjct: 522 PLDGRSTLSGLGYDYGIDPEVAQSSAALHYNGNMKPWLELGIPDYKKYWRRFL 364
>gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0025O15f, mRNA sequence
Length = 674
Score = 80.3 bits (169), Expect(3) = 5e-032
Identities = 35/66 (53%), Positives = 40/66 (60%)
Frame = +3 / +1
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
R LPAGLL F L+ PL+ W GLG+D I IE AA +HYNG MKPWL L I
Sbjct: 328 RTGTLPAGLLTFYGLMEPLDRRWHLLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIH 507
Query: 519 DYKNYW 536
YK+ W
Sbjct: 508 PYKSIW 525
Score = 59.7 bits (124), Expect(3) = 5e-032
Identities = 22/40 (55%), Positives = 34/40 (85%)
Frame = +3 / +1
Query: 42 LLPSLNRVVVLDDDLIVQKDLSPLWNLDMGGKVIGAVQFC 161
+LP+L +VV LDDD++VQKDL+ L+++++ G VIGAV+ C
Sbjct: 19 ILPNLEKVVFLDDDVVVQKDLTQLFSIELHGNVIGAVETC 138
Score = 35.0 bits (70), Expect(3) = 5e-032
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3 / +1
Query: 192 IADHNVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
I +D +C W G+N+ +L WR TSL+
Sbjct: 187 IISSKIDPYTCGWAFGMNIFDLIAWRKANATSLY 288
>gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 5', mRNA sequence
Length = 612
Score = 53.3 bits (110), Expect(4) = 1e-030
Identities = 21/48 (43%), Positives = 32/48 (66%)
Frame = +3 / +2
Query: 51 SLNRVVVLDDDLIVQKDLSPLWNLDMGGKVIGAVQFCGVRLGQLKPYI 194
S +V LD D++V+KD++ LW++DM GKV GAV+ CG + Y+
Sbjct: 17 SSTXMVFLDGDVVVRKDITGLWSIDMKGKVNGAVETCGESFHRFGRYL 160
Score = 51.5 bits (106), Expect(4) = 1e-030
Identities = 15/28 (53%), Positives = 23/28 (82%)
Frame = +3 / +3
Query: 465 ATLHYNGVMKPWLDLGILDYKNYWRKYL 548
A +HYNG +KPWL++G+ +++YW KYL
Sbjct: 444 AVIHYNGNLKPWLEIGLPKFRSYWSKYL 527
Score = 42.3 bits (86), Expect(4) = 1e-030
Identities = 17/42 (40%), Positives = 27/42 (64%)
Frame = +3 / +2
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKA 464
+L LP GL+ F + +PL+ SW GLG++ +S D+E+A
Sbjct: 317 KLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERA 442
Score = 40.5 bits (82), Expect(4) = 1e-030
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3 / +2
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D D+C W G+NV +L +WR IT L+
Sbjct: 188 NFDPDACGWAFGMNVFDLAEWRRQNITELY 277
>gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. vulgare cDNA clone HX14D07
5-PRIME, mRNA sequence
Length = 529
Score = 82.6 bits (174), Expect(3) = 2e-028
Identities = 31/70 (44%), Positives = 44/70 (62%)
Frame = +3 / +2
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
+L LP GL+ F PL+ SW GLG++ IS +I AA +H+NG MKPWLD+G+
Sbjct: 290 KLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMN 469
Query: 519 DYKNYWRKYL 548
++ W KY+
Sbjct: 470 QFRQLWTKYV 499
Score = 53.8 bits (111), Expect(3) = 2e-028
Identities = 19/33 (57%), Positives = 27/33 (81%)
Frame = +3 / +2
Query: 63 VVVLDDDLIVQKDLSPLWNLDMGGKVIGAVQFC 161
++ LDDD++VQ+DL+ LW +DM GKV GAV+ C
Sbjct: 2 ILFLDDDVVVQRDLTGLWKIDMDGKVNGAVETC 100
Score = 26.7 bits (52), Expect(3) = 2e-028
Identities = 7/18 (38%), Positives = 12/18 (66%)
Frame = +3 / +2
Query: 216 DSCVWLSGLNVIELDKWR 269
++C W G+N +L+ WR
Sbjct: 173 NACGWAYGMNFFDLNSWR 226
>gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010D065 5-PRIME, mRNA sequence
Length = 786
Score = 87.7 bits (185), Expect(3) = 1e-027
Identities = 32/70 (45%), Positives = 45/70 (64%)
Frame = +3 / +1
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
+L LP GL+ F L +PL+ SW GLG++ I +I+ AA HYNG MKPWL+L +
Sbjct: 271 KLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMT 450
Query: 519 DYKNYWRKYL 548
Y+ YW +Y+
Sbjct: 451 KYRPYWTRYI 480
Score = 36.8 bits (74), Expect(3) = 1e-027
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = +3 / +1
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D ++C W G+N+ +L +W++ IT ++
Sbjct: 142 NFDPNACGWAYGMNIFDLKQWKNKDITGIY 231
Score = 35.9 bits (72), Expect(3) = 1e-027
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +3 / +1
Query: 96 KDLSPLWNLDMGGKVIGAVQFCGVRLGQLKPYI 194
KDL+ LW++D+ G V GAV CG + Y+
Sbjct: 16 KDLTGLWDVDLNGMVNGAVFTCGESFHRFDKYL 114
>gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 3', mRNA sequence
Length = 643
Score = 90.9 bits (192), Expect(3) = 3e-027
Identities = 32/70 (45%), Positives = 50/70 (71%)
Frame = +3 / -3
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
+L LP GL+ F + +PL+ SW GLG++ +S D+E+AA +HYNG +KPWL++G+
Sbjct: 407 KLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYNGNLKPWLEIGLP 228
Query: 519 DYKNYWRKYL 548
+++YW KYL
Sbjct: 227 KFRSYWSKYL 198
Score = 40.5 bits (82), Expect(3) = 3e-027
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3 / -3
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D D+C W G+NV +L +WR IT L+
Sbjct: 536 NFDPDACGWAFGMNVFDLAEWRRQNITELY 447
Score = 27.6 bits (54), Expect(3) = 3e-027
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +3 / -3
Query: 117 NLDMGGKVIGAVQFCGVRLGQLKPYI 194
++DM GKV GAV+ CG + Y+
Sbjct: 641 SIDMKGKVNGAVETCGESFHRFGRYL 564
>gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. vulgare cDNA clone HX12J15
5-PRIME, mRNA sequence
Length = 653
Score = 90.9 bits (192), Expect(3) = 4e-027
Identities = 32/70 (45%), Positives = 50/70 (71%)
Frame = +3 / -3
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
+L LP GL+ F + +PL+ SW GLG++ +S D+E+AA +HYNG +KPWL++G+
Sbjct: 420 KLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYNGNLKPWLEIGLP 241
Query: 519 DYKNYWRKYL 548
+++YW KYL
Sbjct: 240 KFRSYWSKYL 211
Score = 40.5 bits (82), Expect(3) = 4e-027
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3 / -3
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D D+C W G+NV +L +WR IT L+
Sbjct: 549 NFDPDACGWAFGMNVFDLAEWRRQNITELY 460
Score = 27.2 bits (53), Expect(3) = 4e-027
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3 / -3
Query: 120 LDMGGKVIGAVQFCGVRLGQLKPYI 194
+DM GKV GAV+ CG + Y+
Sbjct: 651 IDMKGKVNGAVETCGESFHRFGRYL 577
>gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B7 5' similar to 68 kDa
protein, mRNA sequence
Length = 616
Score = 87.7 bits (185), Expect(3) = 1e-023
Identities = 32/70 (45%), Positives = 45/70 (64%)
Frame = +3 / +1
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
+L LP GL+ F L +PL+ SW GLG++ I +I+ AA HYNG MKPWL+L +
Sbjct: 232 KLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMT 411
Query: 519 DYKNYWRKYL 548
Y+ YW +Y+
Sbjct: 412 KYRPYWTRYI 441
Score = 36.8 bits (74), Expect(3) = 1e-023
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = +3 / +1
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D ++C W G+N+ +L +W++ IT ++
Sbjct: 103 NFDPNACGWAYGMNIFDLKQWKNKDITGIY 192
Score = 22.1 bits (42), Expect(3) = 1e-023
Identities = 9/25 (36%), Positives = 13/25 (52%)
Frame = +3 / +1
Query: 120 LDMGGKVIGAVQFCGVRLGQLKPYI 194
+D+ G V GAV CG + Y+
Sbjct: 1 VDLNGMVNGAVFTCGESFHRFDKYL 75
>gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 5', mRNA sequence
Length = 630
Score = 93.6 bits (198), Expect(2) = 3e-023
Identities = 36/73 (49%), Positives = 47/73 (64%)
Frame = +3 / +2
Query: 330 KSQRLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDL 509
K +L LP GL+ F +L +PL+ +W GLG+D + +IE AA +HYNG KPWLDL
Sbjct: 233 KLWKLGTLPPGLITFYNLTHPLDHTWHVLGLGYDPAVDIAEIENAAVVHYNGNYKPWLDL 412
Query: 510 GILDYKNYWRKYL 548
I YK YW KY+
Sbjct: 413 AISKYKAYWSKYV 451
Score = 34.1 bits (68), Expect(2) = 3e-023
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +3 / +2
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D +C W G+N+ +L +W+ IT ++
Sbjct: 113 NFDPRACGWAFGMNMFDLKEWKKRNITGIY 202
>gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 3', mRNA sequence
Length = 565
Score = 93.6 bits (198), Expect(2) = 3e-023
Identities = 36/73 (49%), Positives = 47/73 (64%)
Frame = +3 / -1
Query: 330 KSQRLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDL 509
K +L LP GL+ F +L +PL+ +W GLG+D + +IE AA +HYNG KPWLDL
Sbjct: 337 KLWKLGTLPPGLITFYNLTHPLDHTWHVLGLGYDPAVDIAEIENAAVVHYNGNYKPWLDL 158
Query: 510 GILDYKNYWRKYL 548
I YK YW KY+
Sbjct: 157 AISKYKAYWSKYV 119
Score = 34.1 bits (68), Expect(2) = 3e-023
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +3 / -1
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D +C W G+N+ +L +W+ IT ++
Sbjct: 457 NFDPRACGWAFGMNMFDLKEWKKRNITGIY 368
>gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0012C17f, mRNA sequence
Length = 785
Score = 68.9 bits (144), Expect(3) = 4e-023
Identities = 26/64 (40%), Positives = 43/64 (67%)
Frame = +3 / +1
Query: 3 ISIFGLSHFLLPDLLPSLNRVVVLDDDLIVQKDLSPLWNLDMGGKVIGAVQFCGVRLGQL 182
+S+ F LP++ P L+++V LD D++V+KD++ LW++DM GKV GAV+ CG +
Sbjct: 136 LSMLNHLRFYLPEIYPKLDKMVFLDGDVVVRKDITGLWSIDMKGKVNGAVETCGESFHRF 315
Query: 183 KPYI 194
Y+
Sbjct: 316 GRYL 327
Score = 40.5 bits (82), Expect(3) = 4e-023
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3 / +1
Query: 204 NVDDDSCVWLSGLNVIELDKWRDTGITSLH 293
N D D+C W G+NV +L +WR IT L+
Sbjct: 355 NFDPDACGWAFGMNVFDLAEWRRQNITELY 444
Score = 35.4 bits (71), Expect(3) = 4e-023
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +3 / +1
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGIS 443
+L LP GL+ F + +PL+ SW GLG++ +S
Sbjct: 484 KLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVS 588
>gb|AV834211.1|AV834211 AV834211 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags7a24, mRNA sequence
Length = 663
Score = 103 bits (220), Expect = 7e-022
Identities = 41/76 (53%), Positives = 54/76 (71%)
Frame = +3 / -1
Query: 321 DSLKSQRLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPW 500
D S R ALP LL+FQ+LVYPL++ SGLG+ YGI I +A+LHYNG MKPW
Sbjct: 645 DDEASLRAAALPISLLSFQNLVYPLDERLTLSGLGYHYGIEEEVIRTSASLHYNGNMKPW 466
Query: 501 LDLGILDYKNYWRKYL 548
L+LGI +Y+ YW+++L
Sbjct: 465 LELGIPNYRKYWKRFL 418
>gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ001_M07 5', mRNA sequence
Length = 512
Score = 66.1 bits (138), Expect(3) = 3e-021
Identities = 24/53 (45%), Positives = 39/53 (73%)
Frame = +3 / +3
Query: 3 ISIFGLSHFLLPDLLPSLNRVVVLDDDLIVQKDLSPLWNLDMGGKVIGAVQFC 161
+S+ F +P++ P L++VV LDDD++VQKDLS L+ +++ G V+GAV+ C
Sbjct: 51 LSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETC 209
Score = 38.6 bits (78), Expect(3) = 3e-021
Identities = 11/28 (39%), Positives = 20/28 (71%)
Frame = +3 / +3
Query: 210 DDDSCVWLSGLNVIELDKWRDTGITSLH 293
D D+C W G+NV++L +WR+ +T ++
Sbjct: 276 DPDACGWAFGMNVLDLVEWRNKNVTGIY 359
Score = 33.6 bits (67), Expect(3) = 3e-021
Identities = 15/30 (50%), Positives = 18/30 (60%)
Frame = +3 / +3
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGH 428
+L +LP GLLAF LV L W GLG+
Sbjct: 399 KLGSLPPGLLAFYGLVEALNPKWHVLGLGY 488
>gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0018F09f, mRNA sequence
Length = 856
Score = 87.7 bits (185), Expect(2) = 8e-021
Identities = 32/70 (45%), Positives = 45/70 (64%)
Frame = +3 / +1
Query: 339 RLQALPAGLLAFQDLVYPLEDSWVESGLGHDYGISHVDIEKAATLHYNGVMKPWLDLGIL 518
+L LP GL+ F L +PL+ SW GLG++ I +I+ AA HYNG MKPWL+L +
Sbjct: 115 KLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMT 294
Query: 519 DYKNYWRKYL 548
Y+ YW +Y+
Sbjct: 295 KYRPYWTRYI 324
Score = 31.8 bits (63), Expect(2) = 8e-021
Identities = 8/25 (32%), Positives = 17/25 (68%)
Frame = +3 / +1
Query: 219 SCVWLSGLNVIELDKWRDTGITSLH 293
+C W G+N+ +L +W++ IT ++
Sbjct: 1 ACGWAYGMNIFDLKQWKNKDITGIY 75
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 268,977,418
Number of Sequences: 312970
Number of extensions: 4186213
Number of successful extensions: 47700
Number of sequences better than 1.0e-020: 20
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5811236344
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)