TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF11h01.xg.2.1
(328 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA li... 95 5e-032
gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. v... 95 5e-032
gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA li... 89 7e-032
gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm librar... 89 7e-032
gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-a... 95 9e-032
gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgar... 89 1e-031
gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. v... 89 4e-031
gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA li... 95 6e-031
gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-a... 95 2e-030
gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA li... 83 3e-027
gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA li... 83 3e-027
gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-a... 66 1e-023
gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. v... 93 8e-023
gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day ger... 82 1e-022
gb|AV833846.1|AV833846 AV833846 K. Sato unpublished cDNA li... 73 2e-021
>gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 3', mRNA sequence
Length = 643
Score = 95.5 bits (202), Expect(2) = 5e-032
Identities = 31/58 (53%), Positives = 44/58 (75%)
Frame = -3 / -3
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ +YLNF+ P++ F+P+AC WA+G+NVFDL WRR TEL+H + ++NEDG LW
Sbjct: 581 RFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLW 408
Score = 59.7 bits (124), Expect(2) = 5e-032
Identities = 22/40 (55%), Positives = 29/40 (72%)
Frame = -3 / -3
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++F+ T PLD+SWHV+GLGYNP +S + AAV
Sbjct: 395 LPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAV 276
>gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. vulgare cDNA clone HX12J15
5-PRIME, mRNA sequence
Length = 653
Score = 95.5 bits (202), Expect(2) = 5e-032
Identities = 31/58 (53%), Positives = 44/58 (75%)
Frame = -3 / -3
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ +YLNF+ P++ F+P+AC WA+G+NVFDL WRR TEL+H + ++NEDG LW
Sbjct: 594 RFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLW 421
Score = 59.7 bits (124), Expect(2) = 5e-032
Identities = 22/40 (55%), Positives = 29/40 (72%)
Frame = -3 / -3
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++F+ T PLD+SWHV+GLGYNP +S + AAV
Sbjct: 408 LPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAV 289
>gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 5', mRNA sequence
Length = 598
Score = 89.0 bits (188), Expect(2) = 7e-032
Identities = 29/58 (50%), Positives = 41/58 (70%)
Frame = -3 / +2
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ KYLNF+ P + F+PNAC WAYG+N+FDL+ W+ T ++H++ MNED LW
Sbjct: 224 RFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLW 397
Score = 65.7 bits (137), Expect(2) = 7e-032
Identities = 27/40 (67%), Positives = 30/40 (75%)
Frame = -3 / +2
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GLM+FY T PLDKSWHV+GLGYNPSI I +AAV
Sbjct: 410 LPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAV 529
>gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B7 5' similar to 68 kDa
protein, mRNA sequence
Length = 616
Score = 89.0 bits (188), Expect(2) = 7e-032
Identities = 29/58 (50%), Positives = 41/58 (70%)
Frame = -3 / +1
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ KYLNF+ P + F+PNAC WAYG+N+FDL+ W+ T ++H++ MNED LW
Sbjct: 58 RFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLW 231
Score = 65.7 bits (137), Expect(2) = 7e-032
Identities = 27/40 (67%), Positives = 30/40 (75%)
Frame = -3 / +1
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GLM+FY T PLDKSWHV+GLGYNPSI I +AAV
Sbjct: 244 LPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAV 363
>gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006H13f, mRNA sequence
Length = 1018
Score = 95.5 bits (202), Expect(2) = 9e-032
Identities = 31/58 (53%), Positives = 44/58 (75%)
Frame = -3 / +1
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ +YLNF+ P++ F+P+AC WA+G+NVFDL WRR TEL+H + ++NEDG LW
Sbjct: 310 RFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLW 483
Score = 59.7 bits (124), Expect(2) = 9e-032
Identities = 22/40 (55%), Positives = 29/40 (72%)
Frame = -3 / +1
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++F+ T PLD+SWHV+GLGYNP +S + AAV
Sbjct: 496 LPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAV 615
>gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010D065 5-PRIME, mRNA sequence
Length = 786
Score = 89.0 bits (188), Expect(2) = 1e-031
Identities = 29/58 (50%), Positives = 41/58 (70%)
Frame = -3 / +1
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ KYLNF+ P + F+PNAC WAYG+N+FDL+ W+ T ++H++ MNED LW
Sbjct: 97 RFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLW 270
Score = 65.7 bits (137), Expect(2) = 1e-031
Identities = 27/40 (67%), Positives = 30/40 (75%)
Frame = -3 / +1
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GLM+FY T PLDKSWHV+GLGYNPSI I +AAV
Sbjct: 283 LPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAV 402
>gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10L09
5-PRIME, mRNA sequence
Length = 684
Score = 89.0 bits (188), Expect(2) = 4e-031
Identities = 29/58 (50%), Positives = 42/58 (72%)
Frame = -3 / +3
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ KYLNF+ P + F+PNAC WAYG+N+FDLE W++ T ++H++ MNE+ LW
Sbjct: 348 RFDKYLNFSNPNISQNFDPNACGWAYGMNMFDLEEWKKKDITGIYHKWQNMNENRLLW 521
Score = 63.4 bits (132), Expect(2) = 4e-031
Identities = 25/40 (62%), Positives = 30/40 (75%)
Frame = -3 / +3
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GLM+FY T PLDKSWHV+GLGYNP++ I +AAV
Sbjct: 534 LPPGLMTFYKLTHPLDKSWHVLGLGYNPTVEHAEIDTAAV 653
>gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 5', mRNA sequence
Length = 612
Score = 95.5 bits (202), Expect(2) = 6e-031
Identities = 31/58 (53%), Positives = 44/58 (75%)
Frame = -3 / +2
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ +YLNF+ P++ F+P+AC WA+G+NVFDL WRR TEL+H + ++NEDG LW
Sbjct: 143 RFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLW 316
Score = 56.1 bits (116), Expect(2) = 6e-031
Identities = 20/38 (52%), Positives = 27/38 (71%)
Frame = -3 / +2
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSA 9
L GL++F+ T PLD+SWHV+GLGYNP +S + A
Sbjct: 329 LPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERA 442
>gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0012C17f, mRNA sequence
Length = 785
Score = 95.5 bits (202), Expect(2) = 2e-030
Identities = 31/58 (53%), Positives = 44/58 (75%)
Frame = -3 / +1
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ +YLNF+ P++ F+P+AC WA+G+NVFDL WRR TEL+H + ++NEDG LW
Sbjct: 310 RFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLW 483
Score = 55.1 bits (114), Expect(2) = 2e-030
Identities = 19/31 (61%), Positives = 25/31 (80%)
Frame = -3 / +1
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSIS 30
L GL++F+ T PLD+SWHV+GLGYNP +S
Sbjct: 496 LPPGLVTFWNKTFPLDRSWHVLGLGYNPHVS 588
>gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 3', mRNA sequence
Length = 565
Score = 82.6 bits (174), Expect(2) = 3e-027
Identities = 25/58 (43%), Positives = 41/58 (70%)
Frame = -3 / -1
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ YLNF+ P + + F+P AC WA+G+N+FDL+ W++ T ++H + +NED +LW
Sbjct: 502 RFDTYLNFSHPKISENFDPRACGWAFGMNMFDLKEWKKRNITGIYHYWQNLNEDRKLW 329
Score = 56.5 bits (117), Expect(2) = 3e-027
Identities = 21/40 (52%), Positives = 29/40 (72%)
Frame = -3 / -1
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++FY T PLD +WHV+GLGY+P++ I +AAV
Sbjct: 316 LPPGLITFYNLTHPLDHTWHVLGLGYDPAVDIAEIENAAV 197
>gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 5', mRNA sequence
Length = 630
Score = 82.6 bits (174), Expect(2) = 3e-027
Identities = 25/58 (43%), Positives = 41/58 (70%)
Frame = -3 / +2
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ YLNF+ P + + F+P AC WA+G+N+FDL+ W++ T ++H + +NED +LW
Sbjct: 68 RFDTYLNFSHPKISENFDPRACGWAFGMNMFDLKEWKKRNITGIYHYWQNLNEDRKLW 241
Score = 56.5 bits (117), Expect(2) = 3e-027
Identities = 21/40 (52%), Positives = 29/40 (72%)
Frame = -3 / +2
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++FY T PLD +WHV+GLGY+P++ I +AAV
Sbjct: 254 LPPGLITFYNLTHPLDHTWHVLGLGYDPAVDIAEIENAAV 373
>gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0018F09f, mRNA sequence
Length = 856
Score = 65.7 bits (137), Expect(2) = 1e-023
Identities = 27/40 (67%), Positives = 30/40 (75%)
Frame = -3 / +1
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GLM+FY T PLDKSWHV+GLGYNPSI I +AAV
Sbjct: 127 LPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAV 246
Score = 62.0 bits (129), Expect(2) = 1e-023
Identities = 19/38 (50%), Positives = 27/38 (71%)
Frame = -3 / +1
Query: 251 ACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
AC WAYG+N+FDL+ W+ T ++H++ MNED LW
Sbjct: 1 ACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLW 114
>gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. vulgare cDNA clone HX14D07
5-PRIME, mRNA sequence
Length = 529
Score = 92.7 bits (196), Expect(2) = 8e-023
Identities = 30/58 (51%), Positives = 43/58 (74%)
Frame = -3 / +2
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
RY +Y+NF+ P+++ +FNPNAC WAYG+N FDL +WRR+ TE +H + NE+ LW
Sbjct: 116 RYWQYMNFSHPLIKAKFNPNACGWAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLW 289
Score = 31.3 bits (62), Expect(2) = 8e-023
Identities = 13/28 (46%), Positives = 21/28 (75%)
Frame = -2 / +1
Query: 102 LLRQYQATG*VMACNGSWL*SKHQP*GN 19
+L ++A+G ++AC GS +*SKHQ G+
Sbjct: 322 ILLDHKASGQILACPGSRV*SKHQHGGD 405
>gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ001_M07 5', mRNA sequence
Length = 512
Score = 82.2 bits (173), Expect(2) = 1e-022
Identities = 27/58 (46%), Positives = 38/58 (65%)
Frame = -3 / +3
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
R+ KYLN + P++R F+P+AC WA+G+NV DL WR T ++H + E N D LW
Sbjct: 225 RFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDLVEWRNKNVTGIYHYWQERNADHTLW 398
Score = 40.9 bits (83), Expect(2) = 1e-022
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = -3 / +3
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGY 45
L GL++FYG + L+ WHV+GLGY
Sbjct: 411 LPPGLLAFYGLVEALNPKWHVLGLGY 488
>gb|AV833846.1|AV833846 AV833846 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags20p21, mRNA sequence
Length = 494
Score = 73.5 bits (154), Expect(2) = 2e-021
Identities = 23/52 (44%), Positives = 36/52 (69%)
Frame = -3 / +3
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMN 156
R+R Y NF+ P++ +P+ CAWAYG+NVFDLE WR+ + +H +++ N
Sbjct: 33 RFRTYFNFSHPVIDRSLDPDECAWAYGMNVFDLEAWRKTNIRDTYHFWLKEN 188
Score = 46.0 bits (94), Expect(2) = 2e-021
Identities = 17/40 (42%), Positives = 26/40 (65%)
Frame = -3 / +3
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L L++F G+ +D SWH++GLGY S E+++ AAV
Sbjct: 225 LPPALIAFRGHVHGIDPSWHMLGLGYQESTDIESVKKAAV 344
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 194,693,929
Number of Sequences: 312970
Number of extensions: 3446940
Number of successful extensions: 224090
Number of sequences better than 1.0e-020: 18
length of database: 58,378,179
effective HSP length: 48
effective length of database: 43,355,619
effective search space used: 2601337140
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)