TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE36e08.yg.2.1
(352 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF257531.2|BF257531 HVSMEf0013C16f Hordeum vulgare seedl... 174 1e-058
gb|BM370198.2|BM370198 EBro08_SQ003_E17_R root, 3 week, dro... 209 5e-054
gb|BE421314.1|BE421314 HWM008.A07 ITEC HWM Barley Leaf Libr... 175 8e-044
gb|BI956973.1|BI956973 HVSMEn0006I16f Hordeum vulgare rachi... 138 4e-038
gb|BM816903.1|BM816903 HC113F11_SK.ab1 HC Hordeum vulgare s... 93 5e-037
gb|BM375016.1|BM375016 EBem06_SQ002_B13_R embryo, 21 DPA, n... 134 2e-031
emb|AJ234679.1|HVU234679 Hordeum vulgare genomic DNA fragme... 116 5e-026
>gb|BF257531.2|BF257531 HVSMEf0013C16f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0013C16f, mRNA sequence
Length = 495
Score = 174 bits (374), Expect(2) = 1e-058
Identities = 72/88 (81%), Positives = 76/88 (86%)
Frame = -1 / +1
Query: 343 QGXXXXXXGASMXXVSFQLPDTVSLLQAHYHHIPGVFTDDFPALPPVFFDFTSQNVPRAL 164
QG GASM VSFQLP+TVSLLQAHYH IPGVFT+DFPA PPV FD+TSQNVPRAL
Sbjct: 1 QGPNNTRFGASMNNVSFQLPNTVSLLQAHYHRIPGVFTEDFPARPPVVFDYTSQNVPRAL 180
Query: 163 WQPVKGTRLYRVRYGAVVQIVFQDTGIF 80
WQPVKGTRLYRV+ GAVVQIVFQDTGIF
Sbjct: 181 WQPVKGTRLYRVKSGAVVQIVFQDTGIF 264
Score = 171 bits (368), Expect(2) = 1e-054
Identities = 71/93 (76%), Positives = 71/93 (76%)
Frame = +3 / -2
Query: 63 GCSSAAKMPVSWNTICTTAPYLTRYRRVPLTGCHSARGTFCEVKSKNTGGSAGKSSVNTP 242
G KMPVSWNTICTTAP LTRY RVPLTGC SARGTFCEV SK TGG AGKSSV TP
Sbjct: 281 GAPPPRKMPVSWNTICTTAPDLTRYSRVPLTGCQSARGTFCEV*SKTTGGRAGKSSVKTP 102
Query: 243 GMWW*CACSRETVSGSWNDTXXMLAPXXXXXXP 341
GM W*CACSRETV GSWNDT MLAP P
Sbjct: 101 GMRW*CACSRETVLGSWNDTLFMLAPNRVLLGP 3
Score = 86.8 bits (183), Expect(3) = 1e-035
Identities = 37/47 (78%), Positives = 39/47 (82%)
Frame = -3 / +3
Query: 215 APARVLRLHLAERPARAVAARQGHPPVPRQVRRRGADRVPGHGHLRR 75
+PA LRLHLAERP RA+AARQG VPRQVRRRGAD VPGHGHL R
Sbjct: 129 SPAGGLRLHLAERPPRALAARQGDAAVPRQVRRRGADCVPGHGHLSR 269
Score = 69.8 bits (146), Expect(2) = 1e-058
Identities = 24/27 (88%), Positives = 25/27 (92%)
Frame = -1 / +2
Query: 82 FAAEEHPMHIHGYHFYVLATGFGNYDP 2
FA EEHPMHIHGYHFYV ATGFGNY+P
Sbjct: 263 FAGEEHPMHIHGYHFYVFATGFGNYNP 343
Score = 61.1 bits (127), Expect(3) = 9e-022
Identities = 26/35 (74%), Positives = 29/35 (82%)
Frame = +2 / -1
Query: 80 EDARVLEHDLHHGAVPDAVQAGALDGLPQRARDVL 184
+DARVLEH+LHHGA DAVQ LDGLP+RA DVL
Sbjct: 264 KDARVLEHNLHHGAGLDAVQPRPLDGLPERAGDVL 160
Score = 59.3 bits (123), Expect(2) = 1e-054
Identities = 23/27 (85%), Positives = 24/27 (88%)
Frame = +3 / -1
Query: 3 GS*LPNPVAST*KW*PWMCMGCSSAAK 83
G *LP PVA+T*KW*PWMCMGCSS AK
Sbjct: 342 GL*LPKPVANT*KW*PWMCMGCSSPAK 262
Score = 50.6 bits (104), Expect(3) = 1e-035
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = -3 / +1
Query: 89 GHLRRRGAPHAHPRLPLLRAGHGVRQLRP 3
G R GAPHAHPRLPLLR H +RQL+P
Sbjct: 256 GIFRGGGAPHAHPRLPLLRVRHRLRQLQP 342
Score = 49.2 bits (101), Expect(3) = 9e-022
Identities = 21/28 (75%), Positives = 21/28 (75%)
Frame = +2 / -1
Query: 260 RLQQGDGVGELERHXXHARAXXGXXXAL 343
RLQQGDGVGELERH HARA G AL
Sbjct: 84 RLQQGDGVGELERHVVHARAEPGVVGAL 1
Score = 46.9 bits (96), Expect(3) = 1e-035
Identities = 19/28 (67%), Positives = 20/28 (71%)
Frame = -3 / +3
Query: 341 GPXXXXXXREHXXRVVPAPRHRLPAAGA 258
GP REH RVVPAP+HRLPAAGA
Sbjct: 3 GPQQHPVRREHEQRVVPAPQHRLPAAGA 86
Score = 26.7 bits (52), Expect(3) = 9e-022
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = +1 / -2
Query: 4 GRSCRTPWPARRSGS 48
G SCR+ W RRSGS
Sbjct: 341 GCSCRSLWRTRRSGS 297
>gb|BM370198.2|BM370198 EBro08_SQ003_E17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ003_E17 5', mRNA sequence
Length = 638
Score = 209 bits (451), Expect = 5e-054
Identities = 84/99 (84%), Positives = 88/99 (88%)
Frame = -1 / +2
Query: 352 PFCQGXXXXXXGASMXXVSFQLPDTVSLLQAHYHHIPGVFTDDFPALPPVFFDFTSQNVP 173
PFCQG GASM VSFQLP+TVSLLQAHYH IPGVFT+DFPA PPV FD+TSQNVP
Sbjct: 341 PFCQGPNNTRFGASMNNVSFQLPNTVSLLQAHYHRIPGVFTEDFPARPPVVFDYTSQNVP 520
Query: 172 RALWQPVKGTRLYRVRYGAVVQIVFQDTGIFAAEEHPMH 56
RALWQPVKGTRLYRV+YGAVVQIVFQDTGIFAAEEHPMH
Sbjct: 521 RALWQPVKGTRLYRVKYGAVVQIVFQDTGIFAAEEHPMH 637
Score = 205 bits (443), Expect = 6e-053
Identities = 82/98 (83%), Positives = 82/98 (83%)
Frame = +3 / -3
Query: 57 CMGCSSAAKMPVSWNTICTTAPYLTRYRRVPLTGCHSARGTFCEVKSKNTGGSAGKSSVN 236
CMGCSSAAKMPVSWNTICTTAPYLTRY RVPLTGC SARGTFCEV SK TGG AGKSSV
Sbjct: 636 CMGCSSAAKMPVSWNTICTTAPYLTRYSRVPLTGCQSARGTFCEV*SKTTGGRAGKSSVK 457
Query: 237 TPGMWW*CACSRETVSGSWNDTXXMLAPXXXXXXPWQN 350
TPGM W*CACSRETV GSWNDT MLAP PWQN
Sbjct: 456 TPGMRW*CACSRETVLGSWNDTLFMLAPNRVLLGPWQN 343
Score = 98.7 bits (209), Expect(2) = 4e-031
Identities = 42/53 (79%), Positives = 44/53 (83%)
Frame = -3 / +1
Query: 215 APARVLRLHLAERPARAVAARQGHPPVPRQVRRRGADRVPGHGHLRRRGAPHA 57
+PA LRLHLAERP RA+AARQG VPRQVRRRGAD VPGHGHLR GAPHA
Sbjct: 478 SPAGGLRLHLAERPPRALAARQGDAAVPRQVRRRGADCVPGHGHLRGGGAPHA 636
Score = 66.1 bits (138), Expect(2) = 6e-022
Identities = 28/35 (80%), Positives = 30/35 (85%)
Frame = +2 / -2
Query: 80 EDARVLEHDLHHGAVPDAVQAGALDGLPQRARDVL 184
EDARVLEH+LHHGAV DAVQ LDGLP+RA DVL
Sbjct: 613 EDARVLEHNLHHGAVLDAVQPRPLDGLPERAGDVL 509
Score = 55.6 bits (115), Expect(2) = 6e-022
Identities = 24/31 (77%), Positives = 24/31 (77%)
Frame = +2 / -2
Query: 260 RLQQGDGVGELERHXXHARAXXGXXXALAER 352
RLQQGDGVGELERH HARA G ALAER
Sbjct: 433 RLQQGDGVGELERHVVHARAEPGVVGALAER 341
Score = 53.8 bits (111), Expect(2) = 4e-031
Identities = 22/31 (70%), Positives = 23/31 (74%)
Frame = -3 / +1
Query: 350 VLPGPXXXXXXREHXXRVVPAPRHRLPAAGA 258
VLPGP REH RVVPAP+HRLPAAGA
Sbjct: 343 VLPGPQQHPVRREHEQRVVPAPQHRLPAAGA 435
>gb|BE421314.1|BE421314 HWM008.A07 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM008.A07, mRNA sequence
Length = 905
Score = 175 bits (377), Expect = 8e-044
Identities = 65/104 (62%), Positives = 81/104 (77%)
Frame = -1 / +2
Query: 316 ASMXXVSFQLPDTVSLLQAHYHHIPGVFTDDFPALPPVFFDFTSQNVPRALWQPVKGTRL 137
ASM VSF P SLL AHY+ +PGVFT DFPA PPV FD+T+QNV ++LWQP+ T+L
Sbjct: 137 ASMNNVSFVFPKATSLLHAHYYDMPGVFTTDFPAYPPVQFDYTAQNVSQSLWQPIPATKL 316
Query: 136 YRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYD 5
Y++R+G+VVQ+V QDT I E HP+H+HGY FY+LA GFGNYD
Sbjct: 317 YKLRFGSVVQVVLQDTSIVTPENHPIHLHGYDFYILAEGFGNYD 448
Score = 102 bits (218), Expect = 7e-022
Identities = 55/104 (52%), Positives = 57/104 (54%)
Frame = +3 / -3
Query: 6 S*LPNPVAST*KW*PWMCMGCSSAAKMPVSWNTICTTAPYLTRYRRVPLTGCHSARGTFC 185
S*LP P A *K PW MG S M VS T CTT P L+ Y V GCH TFC
Sbjct: 447 S*LPKPSARM*KSYPWRWMGWFSGVTMLVSCRTTCTTEPNLSLYSLVAGIGCHRLWLTFC 268
Query: 186 EVKSKNTGGSAGKSSVNTPGMWW*CACSRETVSGSWNDTXXMLA 317
V S TGG AGKS VNTPGM *CA RE G +T MLA
Sbjct: 267 AV*SNCTGG*AGKSVVNTPGMS**CAWRREVALGKTKETLFMLA 136
>gb|BI956973.1|BI956973 HVSMEn0006I16f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0006I16f, mRNA sequence
Length = 834
Score = 138 bits (297), Expect(2) = 4e-038
Identities = 52/79 (65%), Positives = 63/79 (79%)
Frame = -1 / +1
Query: 241 GVFTDDFPALPPVFFDFTSQNVPRALWQPVKGTRLYRVRYGAVVQIVFQDTGIFAAEEHP 62
GVFT DFPA P V FD+T+QNV RALWQPV GT++Y++ YG+VVQIV Q T IFA E HP
Sbjct: 424 GVFTTDFPANPAVQFDYTAQNVSRALWQPVPGTKVYKLTYGSVVQIVLQGTNIFAGENHP 603
Query: 61 MHIHGYHFYVLATGFGNYD 5
MH+HGY ++LA GFG Y+
Sbjct: 604 MHLHGYELFILAEGFGTYN 660
Score = 97.3 bits (206), Expect(2) = 5e-023
Identities = 44/78 (56%), Positives = 49/78 (62%)
Frame = +3 / -2
Query: 9 *LPNPVAST*KW*PWMCMGCSSAAKMPVSWNTICTTAPYLTRYRRVPLTGCHSARGTFCE 188
*+P P A *PW C+G S AKM V W+TICTT PY++ Y VP TGC AR TFC
Sbjct: 656 *VPKPSARINNS*PWRCIGWFSPAKMLVPWSTICTTEPYVSLYTLVPGTGCQRARLTFCA 477
Query: 189 VKSKNTGGSAGKSSVNTP 242
V S T G AGKS V TP
Sbjct: 476 V*SNCTAGLAGKSVVKTP 423
Score = 37.7 bits (76), Expect(2) = 4e-038
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = -1 / +1
Query: 346 CQGXXXXXXGASMXXVSFQLPDTVSLLQAHY 254
C G AS+ SF LP TVS+LQAHY
Sbjct: 310 CGGPNGTRFAASINNASFVLPSTVSILQAHY 402
Score = 28.6 bits (56), Expect(2) = 5e-023
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +3 / -2
Query: 252 W*CACSRETVSGSWNDTXXMLA 317
W*CA ETV GS + MLA
Sbjct: 404 W*CAWRMETVEGSTKEALLMLA 339
>gb|BM816903.1|BM816903 HC113F11_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC113F11_SK.ab1 similar to Laccase,Catechol
oxidase,L-ascorbate oxidase,, mRNA sequence
Length = 883
Score = 93.2 bits (197), Expect(5) = 5e-037
Identities = 37/49 (75%), Positives = 39/49 (79%)
Frame = -1 / +1
Query: 352 PFCQGXXXXXXGASMXXVSFQLPDTVSLLQAHYHHIPGVFTDDFPALPP 206
PFCQG GASM VSFQLP+TVSLLQAHYH IPGVFT+DFPA PP
Sbjct: 505 PFCQGPNNTRFGASMNNVSFQLPNTVSLLQAHYHRIPGVFTEDFPARPP 651
Score = 90.9 bits (192), Expect(5) = 2e-033
Identities = 36/48 (75%), Positives = 36/48 (75%)
Frame = +3 / -3
Query: 207 GGSAGKSSVNTPGMWW*CACSRETVSGSWNDTXXMLAPXXXXXXPWQN 350
GG AGKSSV TPGM W*CACSRETV GSWNDT MLAP PWQN
Sbjct: 650 GGRAGKSSVKTPGMRW*CACSRETVLGSWNDTLFMLAPNRVLLGPWQN 507
Score = 50.6 bits (104), Expect(5) = 5e-037
Identities = 20/27 (74%), Positives = 21/27 (77%)
Frame = -1 / +2
Query: 220 PALPPVFFDFTSQNVPRALWQPVKGTR 140
P V FD+TSQNVPRALWQPVKG R
Sbjct: 638 PLARRVVFDYTSQNVPRALWQPVKGGR 718
Score = 47.3 bits (97), Expect(5) = 2e-033
Identities = 19/28 (67%), Positives = 22/28 (78%)
Frame = +3 / -2
Query: 141 RVPLTGCHSARGTFCEVKSKNTGGSAGK 224
R PLTGC SARGTFCEV SK T ++G+
Sbjct: 717 RPPLTGCQSARGTFCEV*SKTTRRASGE 634
Score = 30.4 bits (60), Expect(5) = 5e-037
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -1 / +2
Query: 73 EEHPMHIHGY 44
EEHPMH+HGY
Sbjct: 791 EEHPMHMHGY 820
Score = 25.4 bits (49), Expect(5) = 5e-037
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = -1 / +1
Query: 109 QIVFQDTGIFAAEEHP 62
++ FQDTGIFAA P
Sbjct: 754 RLCFQDTGIFAAGGAP 801
Score = 24.9 bits (48), Expect(5) = 2e-033
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +3 / -3
Query: 63 GCSSAAKMPVSW 98
G AAKMPVSW
Sbjct: 800 GAPPAAKMPVSW 765
Score = 24.9 bits (48), Expect(5) = 2e-033
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +3 / -2
Query: 45 *PWMCMGCSS 74
*P MCMGCSS
Sbjct: 819 *PCMCMGCSS 790
Score = 24.0 bits (46), Expect(5) = 5e-037
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -1 / +3
Query: 43 HFYVLATGFGNYDP 2
H YVLATG G P
Sbjct: 822 HLYVLATGLG*LQP 863
Score = 23.1 bits (44), Expect(5) = 2e-033
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +3 / -1
Query: 15 PNPVAST*KW 44
P+PVAST KW
Sbjct: 850 PSPVASTYKW 821
>gb|BM375016.1|BM375016 EBem06_SQ002_B13_R embryo, 21 DPA, no treatment, cv Optic, EBem06
Hordeum vulgare subsp. vulgare cDNA clone
EBem06_SQ002_B13 5', mRNA sequence
Length = 389
Score = 134 bits (287), Expect = 2e-031
Identities = 49/73 (67%), Positives = 61/73 (83%)
Frame = -1 / -3
Query: 223 FPALPPVFFDFTSQNVPRALWQPVKGTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGY 44
FPA PPV FD+T+QNV RALWQPV GT++Y++ YG+VVQIV Q T IFA E HP+H+HGY
Sbjct: 387 FPANPPVQFDYTAQNVSRALWQPVPGTKVYKLTYGSVVQIVLQGTNIFAGENHPIHLHGY 208
Query: 43 HFYVLATGFGNYD 5
F++LA GFGN+D
Sbjct: 207 DFFILAEGFGNFD 169
>emb|AJ234679.1|HVU234679 Hordeum vulgare genomic DNA fragment; clone MWG0961.uni
Length = 510
Score = 116 bits (248), Expect = 5e-026
Identities = 44/72 (61%), Positives = 57/72 (79%)
Frame = -1 / -1
Query: 316 ASMXXVSFQLPDTVSLLQAHYHHIPGVFTDDFPALPPVFFDFTSQNVPRALWQPVKGTRL 137
ASM VSF P SLL AHY+ +PGVFT DFPA PPV FD+T+QNV ++LWQP+ T+L
Sbjct: 216 ASMNNVSFVFPKATSLLHAHYYDMPGVFTTDFPAYPPVQFDYTAQNVSQSLWQPIPATKL 37
Query: 136 YRVRYGAVVQIV 101
Y++R+G+VVQ+V
Sbjct: 36 YKLRFGSVVQVV 1
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 138,157,617
Number of Sequences: 312970
Number of extensions: 2449907
Number of successful extensions: 308517
Number of sequences better than 1.0e-020: 9
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 2883857483
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)