TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8636621.2.1
(691 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL500488.1|AL500488 AL500488 Hordeum vulgare Barke etiol... 166 2e-040
gb|CA030254.1|CA030254 HX06I12r HX Hordeum vulgare subsp. v... 154 6e-037
gb|BI957153.1|BI957153 HVSMEn0007M22f Hordeum vulgare rachi... 148 4e-035
gb|BE060220.1|BE060220 HVSMEg0011K01f Hordeum vulgare pre-a... 133 1e-031
gb|CB874858.1|CB874858 HX06I12w HX Hordeum vulgare subsp. v... 121 4e-027
>gb|AL500488.1|AL500488 AL500488 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK06N15r 3', mRNA sequence
Length = 339
Score = 166 bits (358), Expect(2) = 2e-040
Identities = 73/100 (73%), Positives = 79/100 (79%)
Frame = +1 / +1
Query: 337 CAS*SCSRFLAPFFSALLPHSTK*RPMTISTAPMIPPV*SWSPRMAEAMTATATVCTGWK 516
C + S S +L FFS+L PHSTK P+TISTAPMIPPV SWSPRM AMTATATV TGWK
Sbjct: 37 CLAISGSCWLLVFFSSLFPHSTKYSPITISTAPMIPPVXSWSPRMTAAMTATATVWTGWK 216
Query: 517 TAVKSGPPLSMHQICRANATPDATRPAYRMVKSSPPERTF 636
TAVK GPP SMH I A ATP+AT PA RMVKSSPP+RTF
Sbjct: 217 TAVKRGPPRSMHXIWSAKATPEATSPA*RMVKSSPPDRTF 336
Score = 164 bits (352), Expect = 6e-040
Identities = 72/88 (81%), Positives = 73/88 (82%)
Frame = -3 / -2
Query: 635 KVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQPVQTVAVAVMASAILGDQ 456
KVRSGGELFTILYAGLVASGVAFALQ WCIDRGGPLFTAVFQPVQTVAVAVMA+ ILGDQ
Sbjct: 335 KVRSGGELFTILYAGLVASGVAFALQXWCIDRGGPLFTAVFQPVQTVAVAVMAAVILGDQ 156
Query: 455 LYTXXXXXXXXXXXXLYFVLWGKSAEKK 372
LYT LYFVLWGKS EKK
Sbjct: 155 LYTGGIIGAVLIVIGLYFVLWGKSEEKK 72
Score = 18.9 bits (35), Expect(2) = 2e-040
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +3 / +3
Query: 297 SFSAEQVPRHV 329
S +A QVPRH+
Sbjct: 18 SRAAXQVPRHL 50
>gb|CA030254.1|CA030254 HX06I12r HX Hordeum vulgare subsp. vulgare cDNA clone HX06I12
5-PRIME, mRNA sequence
Length = 651
Score = 154 bits (330), Expect = 6e-037
Identities = 66/102 (64%), Positives = 77/102 (75%)
Frame = +1 / -2
Query: 379 SALLPHSTK*RPMTISTAPMIPPV*SWSPRMAEAMTATATVCTGWKTAVKSGPPLSMHQI 558
S+LLP ST+* P+ +S APMIPP *++SPR+ EAM AT +VCTGW TA +GPP SMHQI
Sbjct: 647 SSLLPQSTR*SPVMMSAAPMIPPR*NFSPRVREAMMATTSVCTGWYTATNTGPPRSMHQI 468
Query: 559 CRANATPDATRPAYRMVKSSPPERTFHLLRSSSVKAAMARNW 684
C ANATPDA +PA RMVK+S PE H S S KAAM RNW
Sbjct: 467 CTANATPDAMKPA*RMVKTSEPEWKTHAPASLSKKAAMMRNW 342
Score = 149 bits (320), Expect = 1e-035
Identities = 61/105 (58%), Positives = 74/105 (70%)
Frame = -3 / +1
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQ 510
++QFL IAAF E D W SG E+FTILYAG +ASGVAFA+QIWCI+RGGP+F AV+Q
Sbjct: 337 VIQFLIIAAFFERDAGAWVFHSGSEVFTILYAGFIASGVAFAVQIWCIERGGPVFVAVYQ 516
Query: 509 PVQTVAVAVMASAILGDQLYTXXXXXXXXXXXXLYFVLWGKSAEK 375
PVQT+ VA+MAS LG++ Y LY VLWGKS E+
Sbjct: 517 PVQTLVVAIMASLTLGEKFYLGGIIGAALIITGLYLVLWGKSEER 651
>gb|BI957153.1|BI957153 HVSMEn0007M22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007M22f, mRNA sequence
Length = 1133
Score = 148 bits (317), Expect = 4e-035
Identities = 64/102 (62%), Positives = 76/102 (74%)
Frame = +1 / -2
Query: 379 SALLPHSTK*RPMTISTAPMIPPV*SWSPRMAEAMTATATVCTGWKTAVKSGPPLSMHQI 558
S+LLP ST+* P+ +S APMIP *++SPR+ +AM AT +VCTGW TA +GPP SMHQI
Sbjct: 526 SSLLPQSTR*SPVMMSAAPMIPLR*NFSPRVRQAMMATTSVCTGWYTATNTGPPRSMHQI 347
Query: 559 CRANATPDATRPAYRMVKSSPPERTFHLLRSSSVKAAMARNW 684
C ANATPDA +PA RMVK+S PE H S S KAAM RNW
Sbjct: 346 CTANATPDAMKPA*RMVKTSEPEWKTHAPASLSKKAAMMRNW 221
Score = 147 bits (316), Expect = 5e-035
Identities = 60/105 (57%), Positives = 73/105 (69%)
Frame = -3 / +3
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQ 510
++QFL IAAF E D W SG E+FTILYAG +ASGVAFA+QIWCI+RGGP+F AV+Q
Sbjct: 216 VIQFLIIAAFFERDAGAWVFHSGSEVFTILYAGFIASGVAFAVQIWCIERGGPVFVAVYQ 395
Query: 509 PVQTVAVAVMASAILGDQLYTXXXXXXXXXXXXLYFVLWGKSAEK 375
PVQT+ VA+MA LG++ Y LY VLWGKS E+
Sbjct: 396 PVQTLVVAIMACLTLGEKFYLSGIIGAALIITGLYLVLWGKSEER 530
>gb|BE060220.1|BE060220 HVSMEg0011K01f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011K01f, mRNA sequence
Length = 645
Score = 133 bits (284), Expect(2) = 1e-031
Identities = 52/80 (65%), Positives = 64/80 (80%)
Frame = -3 / +1
Query: 689 LLQFLAIAAFTEEDLSRWKVRSGGELFTILYAGLVASGVAFALQIWCIDRGGPLFTAVFQ 510
++QFL IAAF E D W SG E+FTILYAG +ASGVAFA+QIWCI+RGGP+F AV+Q
Sbjct: 166 VIQFLIIAAFFERDAGAWVFHSGSEVFTILYAGFIASGVAFAVQIWCIERGGPVFVAVYQ 345
Query: 509 PVQTVAVAVMASAILGDQLY 450
PVQT+ VA+MAS LG++ Y
Sbjct: 346 PVQTLVVAIMASLTLGEKFY 405
Score = 121 bits (258), Expect(2) = 7e-028
Identities = 52/81 (64%), Positives = 59/81 (72%)
Frame = +1 / -2
Query: 442 PPV*SWSPRMAEAMTATATVCTGWKTAVKSGPPLSMHQICRANATPDATRPAYRMVKSSP 621
P *++SPR+ EAM AT +VCTGW TA +GPP SMHQIC ANATPDA +PA RMVK+S
Sbjct: 413 PRR*NFSPRVREAMMATTSVCTGWYTATNTGPPRSMHQICTANATPDAMKPA*RMVKTSE 234
Query: 622 PERTFHLLRSSSVKAAMARNW 684
PE H S S KAAM RNW
Sbjct: 233 PEWKTHAPASLSKKAAMMRNW 171
Score = 23.1 bits (44), Expect(2) = 1e-031
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = -3 / +2
Query: 407 YFVLWGKSAEK 375
Y VLWGKS ++
Sbjct: 449 YLVLWGKSQDR 481
Score = 22.6 bits (43), Expect(2) = 7e-028
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +1 / -1
Query: 379 SALLPHSTK*RPMTISTAPMIP 444
S LLP ST+* + + PMIP
Sbjct: 477 SWLLPQSTR*SRLY*ARLPMIP 412
>gb|CB874858.1|CB874858 HX06I12w HX Hordeum vulgare subsp. vulgare cDNA clone HX06I12
3-PRIME, mRNA sequence
Length = 564
Score = 121 bits (259), Expect = 4e-027
Identities = 49/73 (67%), Positives = 59/73 (80%)
Frame = +1 / +3
Query: 379 SALLPHSTK*RPMTISTAPMIPPV*SWSPRMAEAMTATATVCTGWKTAVKSGPPLSMHQI 558
S+LLP ST+* P+ +S APMIPP *++SPR+ EAM AT +VCTGW TA +GPP SMHQI
Sbjct: 345 SSLLPQSTR*SPVMMSAAPMIPPR*NFSPRVREAMMATTSVCTGWYTATNTGPPRSMHQI 524
Query: 559 CRANATPDATRPA 597
C ANATPDA +PA
Sbjct: 525 CTANATPDAMKPA 563
Score = 107 bits (228), Expect = 7e-023
Identities = 43/74 (58%), Positives = 53/74 (71%)
Frame = -3 / -3
Query: 596 AGLVASGVAFALQIWCIDRGGPLFTAVFQPVQTVAVAVMASAILGDQLYTXXXXXXXXXX 417
AG +ASGVAFA+QIWCI+RGGP+F AV+QPVQT+ VA+MAS LG++ Y
Sbjct: 562 AGFIASGVAFAVQIWCIERGGPVFVAVYQPVQTLVVAIMASLTLGEKFYLGGIIGAALII 383
Query: 416 XXLYFVLWGKSAEK 375
LY VLWGKS E+
Sbjct: 382 TGLYLVLWGKSEER 341
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 332,377,625
Number of Sequences: 312970
Number of extensions: 5589238
Number of successful extensions: 105867
Number of sequences better than 1.0e-020: 8
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7550174202
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)