TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8635646.2.1
(573 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW982540.3|AW982540 HVSMEg0003I17f Hordeum vulgare pre-a... 147 6e-035
gb|BQ465733.1|BQ465733 HU04I14r HU Hordeum vulgare subsp. v... 147 6e-035
gb|BQ663904.1|BQ663904 HU04I14u HU Hordeum vulgare subsp. v... 147 6e-035
gb|BG343189.2|BG343189 HVSMEg0005A17f Hordeum vulgare pre-a... 143 1e-033
gb|BQ467236.1|BQ467236 HS03B22r HS Hordeum vulgare subsp. v... 143 1e-033
gb|BF259589.2|BF259589 HVSMEf0019L18f Hordeum vulgare seedl... 140 7e-033
gb|BQ765873.1|BQ765873 EBro03_SQ008_L11_R root, 3 week, wat... 111 4e-024
gb|CV063762.1|CV063762 BNEL92h4 Barley EST endosperm librar... 108 3e-023
gb|CK124817.1|CK124817 BES1824110h18 BES1824 Hordeum vulgar... 108 3e-023
>gb|AW982540.3|AW982540 HVSMEg0003I17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003I17f, mRNA sequence
Length = 534
Score = 147 bits (315), Expect = 6e-035
Identities = 59/89 (66%), Positives = 69/89 (77%)
Frame = +2 / +2
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 65 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 244
Query: 182 EGSLVCPDTSRCFPINKGIPNMMLHEDEV 268
EG+LVCPD+ R FPI KG+PNM+LHEDEV
Sbjct: 245 EGTLVCPDSGRSFPILKGVPNMLLHEDEV 331
Score = 122 bits (260), Expect = 2e-027
Identities = 57/95 (60%), Positives = 62/95 (65%)
Frame = -1 / -1
Query: 285 LTRQNQTSSSWSIMLGMPLLMGKQRLVSGQTREPSWT*TSRSAWWMRRMALPSTSAAPSA 106
L Q +TSSSW +LG P MGK+R +SGQTR PS T SRSAWW RR+ALPSTSAAPSA
Sbjct: 348 LASQCRTSSSWRSILGTPFRMGKERPLSGQTRVPSSTWISRSAWWRRRIALPSTSAAPSA 169
Query: 105 KIXXXXXXXXXXXXXRPTARVAATSARQSILGRRP 1
K P A VAA SARQSILGR P
Sbjct: 168 KSSASGGCSAASSSGNPAALVAAKSARQSILGRIP 64
>gb|BQ465733.1|BQ465733 HU04I14r HU Hordeum vulgare subsp. vulgare cDNA clone HU04I14
5-PRIME, mRNA sequence
Length = 507
Score = 147 bits (315), Expect = 6e-035
Identities = 59/89 (66%), Positives = 69/89 (77%)
Frame = +2 / +2
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 212 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 391
Query: 182 EGSLVCPDTSRCFPINKGIPNMMLHEDEV 268
EG+LVCPD+ R FPI KG+PNM+LHEDEV
Sbjct: 392 EGTLVCPDSGRSFPILKGVPNMLLHEDEV 478
Score = 122 bits (260), Expect = 2e-027
Identities = 57/95 (60%), Positives = 62/95 (65%)
Frame = -1 / -1
Query: 285 LTRQNQTSSSWSIMLGMPLLMGKQRLVSGQTREPSWT*TSRSAWWMRRMALPSTSAAPSA 106
L Q +TSSSW +LG P MGK+R +SGQTR PS T SRSAWW RR+ALPSTSAAPSA
Sbjct: 495 LASQCRTSSSWRSILGTPFRMGKERPLSGQTRVPSSTWISRSAWWRRRIALPSTSAAPSA 316
Query: 105 KIXXXXXXXXXXXXXRPTARVAATSARQSILGRRP 1
K P A VAA SARQSILGR P
Sbjct: 315 KSSASGGCSAASSSGNPAALVAAKSARQSILGRIP 211
>gb|BQ663904.1|BQ663904 HU04I14u HU Hordeum vulgare subsp. vulgare cDNA clone HU04I14
3-PRIME, mRNA sequence
Length = 499
Score = 147 bits (315), Expect = 6e-035
Identities = 59/89 (66%), Positives = 69/89 (77%)
Frame = +2 / -2
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 417 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 238
Query: 182 EGSLVCPDTSRCFPINKGIPNMMLHEDEV 268
EG+LVCPD+ R FPI KG+PNM+LHEDEV
Sbjct: 237 EGTLVCPDSGRSFPILKGVPNMLLHEDEV 151
Score = 122 bits (260), Expect = 2e-027
Identities = 57/95 (60%), Positives = 62/95 (65%)
Frame = -1 / +2
Query: 285 LTRQNQTSSSWSIMLGMPLLMGKQRLVSGQTREPSWT*TSRSAWWMRRMALPSTSAAPSA 106
L Q +TSSSW +LG P MGK+R +SGQTR PS T SRSAWW RR+ALPSTSAAPSA
Sbjct: 134 LASQCRTSSSWRSILGTPFRMGKERPLSGQTRVPSSTWISRSAWWRRRIALPSTSAAPSA 313
Query: 105 KIXXXXXXXXXXXXXRPTARVAATSARQSILGRRP 1
K P A VAA SARQSILGR P
Sbjct: 314 KSSASGGCSAASSSGNPAALVAAKSARQSILGRIP 418
>gb|BG343189.2|BG343189 HVSMEg0005A17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0005A17f, mRNA sequence
Length = 958
Score = 143 bits (306), Expect = 1e-033
Identities = 58/89 (65%), Positives = 68/89 (76%)
Frame = +2 / +2
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 227 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 406
Query: 182 EGSLVCPDTSRCFPINKGIPNMMLHEDEV 268
EG+LVCPD+ R FPI KG+ NM+LHEDEV
Sbjct: 407 EGTLVCPDSGRSFPILKGVXNMLLHEDEV 493
Score = 118 bits (253), Expect = 2e-026
Identities = 56/95 (58%), Positives = 61/95 (64%)
Frame = -1 / -2
Query: 285 LTRQNQTSSSWSIMLGMPLLMGKQRLVSGQTREPSWT*TSRSAWWMRRMALPSTSAAPSA 106
L Q +TSSSW +L P MGK+R +SGQTR PS T SRSAWW RR+ALPSTSAAPSA
Sbjct: 510 LASQCRTSSSWRSILXTPFRMGKERPLSGQTRVPSSTWISRSAWWRRRIALPSTSAAPSA 331
Query: 105 KIXXXXXXXXXXXXXRPTARVAATSARQSILGRRP 1
K P A VAA SARQSILGR P
Sbjct: 330 KSSASGGCSAASSSGNPAALVAAKSARQSILGRIP 226
>gb|BQ467236.1|BQ467236 HS03B22r HS Hordeum vulgare subsp. vulgare cDNA clone HS03B22
5-PRIME, mRNA sequence
Length = 539
Score = 143 bits (306), Expect = 1e-033
Identities = 57/87 (65%), Positives = 67/87 (77%)
Frame = +2 / +2
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 278 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 457
Query: 182 EGSLVCPDTSRCFPINKGIPNMMLHED 262
EG+LVCPD+ R FPI KG+PNM+LHED
Sbjct: 458 EGTLVCPDSGRSFPILKGVPNMLLHED 538
Score = 115 bits (245), Expect = 3e-025
Identities = 53/87 (60%), Positives = 57/87 (65%)
Frame = -1 / -3
Query: 261 SSWSIMLGMPLLMGKQRLVSGQTREPSWT*TSRSAWWMRRMALPSTSAAPSAKIXXXXXX 82
SSW +LG P MGK+R +SGQTR PS T SRSAWW RR+ALPSTSAAPSAK
Sbjct: 537 SSWRSILGTPFRMGKERPLSGQTRVPSSTWISRSAWWRRRIALPSTSAAPSAKSSASGGC 358
Query: 81 XXXXXXXRPTARVAATSARQSILGRRP 1
P A VAA SARQSILGR P
Sbjct: 357 SAASSSGNPAALVAAKSARQSILGRIP 277
>gb|BF259589.2|BF259589 HVSMEf0019L18f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0019L18f, mRNA sequence
Length = 572
Score = 140 bits (300), Expect = 7e-033
Identities = 57/89 (64%), Positives = 67/89 (75%)
Frame = +2 / +3
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 186 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 365
Query: 182 EGSLVCPDTSRCFPINKGIPNMMLHEDEV 268
EG+LVCPD+ FPI KG+PNM+L EDEV
Sbjct: 366 EGTLVCPDSGPSFPILKGVPNMLLQEDEV 452
Score = 109 bits (232), Expect = 2e-023
Identities = 55/95 (57%), Positives = 58/95 (61%)
Frame = -1 / -2
Query: 285 LTRQNQTSSSWSIMLGMPLLMGKQRLVSGQTREPSWT*TSRSAWWMRRMALPSTSAAPSA 106
L Q TSSS +LG P MGK +SGQTR PS T SRSAWW RR+ALPSTSAAPSA
Sbjct: 469 LASQCCTSSS*RSILGTPFRMGKDGPLSGQTRVPSSTWISRSAWWRRRIALPSTSAAPSA 290
Query: 105 KIXXXXXXXXXXXXXRPTARVAATSARQSILGRRP 1
K P A VAA SARQSILGR P
Sbjct: 289 KSSASGGCSAASSSGNPAALVAAKSARQSILGRIP 185
>gb|BQ765873.1|BQ765873 EBro03_SQ008_L11_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_L11 5', mRNA sequence
Length = 433
Score = 111 bits (236), Expect = 4e-024
Identities = 45/71 (63%), Positives = 53/71 (74%)
Frame = +2 / +3
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 219 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 398
Query: 182 EGSLVCPDTSR 214
EG+LVCPD+ R
Sbjct: 399 EGTLVCPDSGR 431
>gb|CV063762.1|CV063762 BNEL92h4 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL92h4 5' similar to unknown
protein, mRNA sequence
Length = 470
Score = 108 bits (230), Expect = 3e-023
Identities = 44/68 (64%), Positives = 51/68 (75%)
Frame = +2 / +2
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 266 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 445
Query: 182 EGSLVCPD 205
EG+LVCPD
Sbjct: 446 EGTLVCPD 469
>gb|CK124817.1|CK124817 BES1824110h18 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010H1810 5-PRIME, mRNA sequence
Length = 432
Score = 108 bits (230), Expect = 3e-023
Identities = 44/68 (64%), Positives = 51/68 (75%)
Frame = +2 / +3
Query: 2 GLLPKIDWRALVAATRAVGXXXXXXXXXXXXXXIFADGAADVEGSAIRRIHHALLEVHVQ 181
G+LPKIDWRAL AAT A G +FA+GAADVEGSAIRR+HHALLE+HV+
Sbjct: 228 GILPKIDWRALFAATSAAGFPELLAAEQPPEAELFAEGAADVEGSAIRRLHHALLEIHVE 407
Query: 182 EGSLVCPD 205
EG+LVCPD
Sbjct: 408 EGTLVCPD 431
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 194,539,379
Number of Sequences: 312970
Number of extensions: 2897961
Number of successful extensions: 42785
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 5895922551
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)