TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5704661.2.1
(798 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-a... 392 e-108
gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA li... 299 e-107
gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA li... 371 e-102
gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. v... 369 e-101
gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-a... 337 5e-097
gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA li... 344 4e-094
gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. v... 325 1e-088
gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. v... 305 2e-082
gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgar... 299 1e-080
gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA li... 283 7e-076
gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA li... 283 7e-076
gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm librar... 283 9e-076
gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green... 259 1e-068
gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day ger... 242 2e-063
gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA li... 233 1e-060
gb|AV931472.1|AV931472 AV931472 K. Sato unpublished cDNA li... 231 4e-060
gb|BU968630.1|BU968630 HB08B19r BC Hordeum vulgare subsp. v... 197 4e-059
gb|BU979822.1|BU979822 HA17H16r HA Hordeum vulgare subsp. v... 224 4e-058
gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day ger... 221 5e-057
gb|BJ549963.1|BJ549963 BJ549963 K. Sato unpublished cDNA li... 220 7e-057
gb|BM444949.1|BM444949 EBem09_SQ006_P20_R embryo, 1 Day ger... 217 5e-056
gb|AV930935.1|AV930935 AV930935 K. Sato unpublished cDNA li... 214 6e-055
gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-a... 212 2e-054
gb|BG417087.1|BG417087 HVSMEk0016D06f Hordeum vulgare testa... 190 7e-048
gb|AV837092.1|AV837092 AV837092 K. Sato unpublished cDNA li... 183 1e-045
gb|BJ468235.1|BJ468235 BJ468235 K. Sato unpublished cDNA li... 178 4e-044
gb|BI777829.2|BI777829 EBro08_SQ002_D17_R root, 3 week, dro... 131 4e-043
gb|AV833832.1|AV833832 AV833832 K. Sato unpublished cDNA li... 158 2e-042
gb|CA018769.1|CA018769 HV09J15r HV Hordeum vulgare subsp. v... 171 3e-042
gb|BF266558.3|BF266558 HV_CEa0015H07f Hordeum vulgare seedl... 149 1e-041
gb|AV909171.1|AV909171 AV909171 K. Sato unpublished cDNA li... 168 4e-041
gb|AV911054.1|AV911054 AV911054 K. Sato unpublished cDNA li... 168 4e-041
gb|BJ544949.1|BJ544949 BJ544949 K. Sato unpublished cDNA li... 168 4e-041
gb|BJ546433.1|BJ546433 BJ546433 K. Sato unpublished cDNA li... 168 4e-041
gb|BM372595.2|BM372595 EBpi05_SQ003_G13_R pistil, 8 DPA, no... 163 9e-040
gb|BJ463761.1|BJ463761 BJ463761 K. Sato unpublished cDNA li... 162 2e-039
gb|AV833846.1|AV833846 AV833846 K. Sato unpublished cDNA li... 84 5e-038
gb|CK124684.1|CK124684 BES1824109p11 BES1824 Hordeum vulgar... 127 4e-037
gb|BJ467937.1|BJ467937 BJ467937 K. Sato unpublished cDNA li... 100 1e-035
gb|CB881764.1|CB881764 HM10N01w HM Hordeum vulgare subsp. v... 146 2e-034
gb|AJ432742.1|AJ432742 AJ432742 S00002 Hordeum vulgare subs... 127 5e-029
gb|AV834161.1|AV834161 AV834161 K. Sato unpublished cDNA li... 97 7e-027
gb|BU986456.1|BU986456 HF11O19r HF Hordeum vulgare subsp. v... 117 8e-026
gb|AV834211.1|AV834211 AV834211 K. Sato unpublished cDNA li... 112 2e-024
gb|BJ463762.1|BJ463762 BJ463762 K. Sato unpublished cDNA li... 111 5e-024
gb|BQ460226.1|BQ460226 HA06N23r HA Hordeum vulgare subsp. v... 107 9e-023
gb|AL510176.1|AL510176 AL510176 Hordeum vulgare Barke devel... 74 3e-021
>gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006H13f, mRNA sequence
Length = 1018
Score = 392 bits (850), Expect = e-108
Identities = 153/205 (74%), Positives = 172/205 (83%)
Frame = -2 / +1
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLPE++PKLDKMVFLD D+VV+KD+ GLWSI+MKGKVNGAVETCGE
Sbjct: 121 RNPKYLSMLNHLRFYLPEIYPKLDKMVFLDGDVVVRKDITGLWSIDMKGKVNGAVETCGE 300
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
SFHR+ RYLNFS+P+I K+FDP AC WAFGMNVFDLAEWRRQNITE+YH WQKLNED L
Sbjct: 301 SFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLL 480
Query: 428 WKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGL 249
WKLGTLP LVTFWNKTFPL RSWHVLGLGYNPHV+SRD+E AAVIHY G++KPWLE GL
Sbjct: 481 WKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYXGHLKPWLEXGL 660
Query: 248 PKFRSYWSKYLDYDQSFLRECNINP 174
P F + L L +INP
Sbjct: 661 PSFEATGQSTLTMIXPXLAGMHINP 735
Score = 280 bits (605), Expect(2) = 1e-076
Identities = 126/166 (75%), Positives = 131/166 (78%)
Frame = +1 / -3
Query: 244 LGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRPRTCHDRLRGNVLFQNVTKHKGRVPS 423
LGRPIS+QGF P *CMTAA S SLLLT GL*PRPRTCHDR GNVLFQNVT+ GRVPS
Sbjct: 665 LGRPISSQGFRCPX*CMTAARSRSLLLTCGL*PRPRTCHDRSXGNVLFQNVTRPGGRVPS 486
Query: 424 FQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSKLLVIMGFEKFRYRS*RW 603
F S SSLSFC + * SVMFCRLHSARSNTFIPKAQ A GSK I G EKF+ R RW
Sbjct: 485 FHSSPSSLSFCQKW*SSVMFCRLHSARSNTFIPKAQPHASGSKFFAITGLEKFK*RPKRW 306
Query: 604 KLSPQVSTAPFTFPFMLIDQRPARSFFTTMSSSRKTILSSFGCISG 741
KLSPQVSTAP TFPF+ IDQRP SF TT S SRKTILSSFG ISG
Sbjct: 305 KLSPQVSTAPLTFPFISIDQRPVMSFLTTTSPSRKTILSSFG*ISG 168
Score = 251 bits (542), Expect(2) = 3e-067
Identities = 107/173 (61%), Positives = 136/173 (78%)
Frame = +3 / -2
Query: 258 LQPGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP*PAEGKCLVPECDQTQRESTKFPE*S 437
L+PGL V VMYDC L VS M +VT*T+NMP* E +CLVPEC+QT RES KFP S
Sbjct: 651 LKPGLQVPXVMYDCGTLQVSTAYMWVVT*TENMP*SIEXECLVPECNQTWRESAKFP*QS 472
Query: 438 IFIKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSMWVKTLGDNGI*KIQVSIIAMETLST 617
+ +KLLP+V+ FSYVLPPPFSQ+KH+HSKSP T + VK L +N *+IQV+ AME LST
Sbjct: 471 VLVKLLPKVVEFSYVLPPPFSQIKHVHSKSPATCIGVKILCNNRT*EIQVTAKAMEALST 292
Query: 618 SLNSTVYLPLHVD*PKASQILLYYDVIIKKDHLVKLWMHLR*VKAEMIED*QV 776
SL ST+ LPLH+D*P+AS +L YY + IKKDHLV+LW++LR V+++MI+ *++
Sbjct: 291 SLYSTINLPLHID*PEASYVLSYYYIAIKKDHLVELWINLRQVESQMIKH*KI 133
Score = 222 bits (480), Expect = 1e-057
Identities = 107/178 (60%), Positives = 118/178 (66%)
Frame = +2 / -1
Query: 245 WVDQSPTRASCCHCNV*LQHAQCLCC*HGDCNLDQEHAMTG*GEMSCSRM*PNTKGEYQV 424
W S RAS CNV*L+HA L C H CNLD+EHAM MSCSRM*P+ +GE QV
Sbjct: 664 WGGXSQARASGAXCNV*LRHAPGLYCLHVGCNLDREHAMIDRXGMSCSRM*PDLEGECQV 485
Query: 425 SRVIYLH*ASATSDKFQLCSAASIQPGQTHSFQKPKHKHVGQNSW**WDLKNSGIDHSDG 604
S + *ASA S + QLCSAASIQP QT SFQKP H H GQNS * DL+NS SDG
Sbjct: 484 SIAVRPR*ASAKSGRVQLCSAASIQPDQTRSFQKPSHMHRGQNSLQ*QDLRNSSNGQSDG 305
Query: 605 NSLHKSQQHRLPSPSC*LTKGQPDPSLLRCHHQERPSCQALDASQVGKSGDD*GLTGT 778
+SLHKS QH PSPS LT+GQ P LL HQERPSC+ALD SQ + DD* L T
Sbjct: 304 SSLHKSLQHH*PSPSYRLTRGQLCPFLLLHRHQERPSCRALDKSQASRISDD*ALKDT 131
Score = 217 bits (468), Expect(2) = 4e-063
Identities = 108/181 (59%), Positives = 122/181 (67%)
Frame = -1 / +3
Query: 798 FERPPGQVPVNPQSSPLLPT*DASKA*QDGLS***HRSKEGSGWPLVNQHEGEGKRCC*D 619
FE +V N SS +L *D SKA QDGLS* SK+G WPLVN++EGEG CC D
Sbjct: 111 FEVQKPKVSFNA*SSEILLA*DLSKARQDGLS*WRCSSKKGHNWPLVNRYEGEG*WCCRD 290
Query: 618 LWREFPSL*SIPEFFKSHYHQEF*PTCLCLGFWNECV*PG*MEAAEHN*NLSLVAEA**R 439
LWRE PSL + EF KS Y +EF*P C+ LGFWNE V* G*MEAAEHN* L L+AEA*
Sbjct: 291 LWRELPSLWPLLEFLKSCYCKEF*PRCMWLGFWNERV*SG*MEAAEHN*TLPLLAEA*RG 470
Query: 438 *ITLETWYSPFVFGHILEQDISPQPVMACSWSRLQSPC*QQRH*ACCSHTLQWQHEALVG 259
+ETW+SP G+ILEQDI + +MACS SRLQ C Q R AC SHTL W EAL
Sbjct: 471 RTAMETWHSPSRSGYILEQDIPXRSIMACSRSRLQPTCKQ*RPGACRSHTLXWAPEALA* 650
Query: 258 D 256
D
Sbjct: 651 D 653
Score = 183 bits (395), Expect = 8e-046
Identities = 102/175 (58%), Positives = 107/175 (61%)
Frame = -3 / +2
Query: 769 QSSIISAFTYLRCIQSLTRWSFLMMTS**RRIWLAFGQST*RGXXXXXXXXXXXVSIAMI 590
Q II T LR IQS TRWSFLM ** R LA GQS *RG SIA+
Sbjct: 140 QCLII*DSTCLRFIQSSTRWSFLMAM***ERT*LASGQSI*RGRLMVL*RLVERASIALA 319
Query: 589 DT*IFQIPLSPRVLTHMLVFGLLE*MCLTWLNGGGRT*LKFITRGRSLMKIDHSGNLVLS 410
T*I Q+ L R+LT M V GLLE* CL WLNGGGRT*L T GRSL + D GNL LS
Sbjct: 320 VT*ISQVLLLQRILTPMHVAGLLE*TCLIWLNGGGRT*LNSTTFGRSLTRTDCYGNLALS 499
Query: 409 LCVWSHSGTRHFPSAGHGMFLV*VTIPMLTAETLSMLQSYITMAT*SPGWRLVYP 245
L VW HSGTRH S HGMF V*VT M ET S+ QSYIT T*SPG R P
Sbjct: 500 LQVWLHSGTRHSXSIDHGMFSV*VTTHM*AVETWSVPQSYITXGT*SPGLRXASP 664
Score = 44.6 bits (91), Expect(2) = 4e-063
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = -2 / +3
Query: 248 PKFRSYWSKYLDYDQSF 198
P+FRSYWSKYLDYDQ F
Sbjct: 660 PQFRSYWSKYLDYDQXF 710
Score = 26.7 bits (52), Expect(2) = 1e-076
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = +1 / -1
Query: 208 S*SRYLDQ*LRNLG 249
S*SRY DQ*LRN G
Sbjct: 700 S*SRYFDQ*LRNWG 659
Score = 24.4 bits (47), Expect(2) = 3e-067
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = +3 / -3
Query: 204 LVIIQILGPVAPKLG 248
L+I+++L PVA KLG
Sbjct: 704 LIIVKVL*PVASKLG 660
>gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 5', mRNA sequence
Length = 612
Score = 299 bits (647), Expect(2) = e-107
Identities = 114/138 (82%), Positives = 125/138 (90%)
Frame = -2 / +2
Query: 713 MVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFDPHAC 534
MVFLD D+VV+KD+ GLWSI+MKGKVNGAVETCGESFHR+ RYLNFS+P+I K+FDP AC
Sbjct: 29 MVFLDGDVVVRKDITGLWSIDMKGKVNGAVETCGESFHRFGRYLNFSSPVIAKNFDPDAC 208
Query: 533 VWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWH 354
WAFGMNVFDLAEWRRQNITE+YH WQKLNED LWKLGTLP LVTFWNKTFPL RSWH
Sbjct: 209 GWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWH 388
Query: 353 VLGLGYNPHVNSRDIEHA 300
VLGLGYNPHV+SRD+E A
Sbjct: 389 VLGLGYNPHVSSRDLERA 442
Score = 232 bits (500), Expect(2) = 1e-080
Identities = 104/139 (74%), Positives = 108/139 (77%)
Frame = +1 / -1
Query: 301 ACSMSLLLTWGL*PRPRTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVM 480
A S SLLLT GL*PRPRTCHDR RGNVLFQNVT+ GRVPSF S SSLSFC + * SVM
Sbjct: 441 ARSRSLLLTCGL*PRPRTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVM 262
Query: 481 FCRLHSARSNTFIPKAQTQACGSKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPFMLID 660
FCRLHSARSNTFIPKAQ A GSK I G EKF+ R RWKLSPQVSTAP TFPF+ ID
Sbjct: 261 FCRLHSARSNTFIPKAQPHASGSKFFAITGLEKFK*RPKRWKLSPQVSTAPLTFPFISID 82
Query: 661 QRPARSFFTTMSSSRKTIL 717
QRP SF TT S SRKT L
Sbjct: 81 QRPVMSFLTTTSPSRKTXL 25
Score = 205 bits (442), Expect(2) = 2e-067
Identities = 88/136 (64%), Positives = 107/136 (78%)
Frame = +3 / -3
Query: 306 LNVSAVNMGIVT*TKNMP*PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVL 485
L VS M +VT*T+NMP* EG+CLVPEC+QT RES KFP S+ +KLLP+V+ FSYVL
Sbjct: 436 LQVSTAYMWVVT*TENMP*SIEGECLVPECNQTWRESAKFP*QSVLVKLLPKVVEFSYVL 257
Query: 486 PPPFSQVKHIHSKSPNTSMWVKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD*PK 665
PPPFSQ+KH+HSKSP T + VK L +N *+IQV+ AME LSTSL ST+ LPLH+D*P+
Sbjct: 256 PPPFSQIKHVHSKSPATCIGVKILCNNRT*EIQVTAKAMEALSTSLYSTINLPLHID*PE 77
Query: 666 ASQILLYYDVIIKKDH 713
AS +L YY + IKKDH
Sbjct: 76 ASYVLSYYYIAIKKDH 29
Score = 182 bits (393), Expect(2) = 7e-059
Identities = 86/137 (62%), Positives = 98/137 (71%)
Frame = -1 / +1
Query: 711 GLS***HRSKEGSGWPLVNQHEGEGKRCC*DLWREFPSL*SIPEFFKSHYHQEF*PTCLC 532
GLS* SK+G WPLVN++EGEG CC DLWRE PSL + EF KS Y +EF*P C+
Sbjct: 31 GLS*WRCSSKKGHNWPLVNRYEGEG*WCCRDLWRELPSLWPLLEFLKSCYCKEF*PRCMW 210
Query: 531 LGFWNECV*PG*MEAAEHN*NLSLVAEA**R*ITLETWYSPFVFGHILEQDISPQPVMAC 352
LGFWNE V* G*MEAAEHN* L L+AEA* +ETW+SP G+ILEQDI P+ +MAC
Sbjct: 211 LGFWNERV*SG*MEAAEHN*TLPLLAEA*RGRTAMETWHSPSRSGYILEQDIPPRSIMAC 390
Query: 351 SWSRLQSPC*QQRH*AC 301
S SRLQ C Q R AC
Sbjct: 391 SRSRLQPTCKQ*RPGAC 441
Score = 180 bits (388), Expect(2) = 9e-058
Identities = 86/137 (62%), Positives = 94/137 (68%)
Frame = +2 / -2
Query: 302 HAQCLCC*HGDCNLDQEHAMTG*GEMSCSRM*PNTKGEYQVSRVIYLH*ASATSDKFQLC 481
HA L C H CNLD+EHAM G MSCSRM*P+ +GE QVS + *ASA S + QLC
Sbjct: 440 HAPGLYCLHVGCNLDREHAMIDRGGMSCSRM*PDLEGECQVSIAVRPR*ASAKSGRVQLC 261
Query: 482 SAASIQPGQTHSFQKPKHKHVGQNSW**WDLKNSGIDHSDGNSLHKSQQHRLPSPSC*LT 661
SAASIQP QT SFQKP H H GQNS * DL+NS SDG+SLHKS QH PSPS LT
Sbjct: 260 SAASIQPDQTRSFQKPSHMHRGQNSLQ*QDLRNSSNGQSDGSSLHKSLQHH*PSPSYRLT 81
Query: 662 KGQPDPSLLRCHHQERP 712
+GQ P LL HQERP
Sbjct: 80 RGQLCPFLLLHRHQERP 30
Score = 149 bits (320), Expect(2) = 4e-047
Identities = 80/137 (58%), Positives = 85/137 (62%)
Frame = -3 / +3
Query: 712 WSFLMMTS**RRIWLAFGQST*RGXXXXXXXXXXXVSIAMIDT*IFQIPLSPRVLTHMLV 533
WSFLM ** R LA GQS *RG SIA+ T*I Q+ L R+LT M V
Sbjct: 30 WSFLMAM***ERT*LASGQSI*RGRLMVL*RLVERASIALAVT*ISQVLLLQRILTPMHV 209
Query: 532 FGLLE*MCLTWLNGGGRT*LKFITRGRSLMKIDHSGNLVLSLCVWSHSGTRHFPSAGHGM 353
GLLE* CL WLNGGGRT*L T GRSL + D GNL LSL VW HSGTRH PS HGM
Sbjct: 210 AGLLE*TCLIWLNGGGRT*LNSTTFGRSLTRTDCYGNLALSLQVWLHSGTRHSPSIDHGM 389
Query: 352 FLV*VTIPMLTAETLSM 302
F V*VT M ET S+
Sbjct: 390 FSV*VTTHM*AVETWSV 440
Score = 110 bits (234), Expect(2) = e-107
Identities = 41/43 (95%), Positives = 42/43 (97%)
Frame = -2 / +3
Query: 299 AVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP* 171
AVIHYNGN+KPWLEIGLPKFRSYWSKYLDYDQ FLRECNINP*
Sbjct: 444 AVIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLRECNINP* 572
Score = 88.6 bits (187), Expect(2) = 1e-080
Identities = 39/44 (88%), Positives = 40/44 (90%)
Frame = +1 / -3
Query: 169 LHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTA 300
LHGLMLHSRRK S*SRY DQ*LRNLGRPIS+QGF LPL*CMTA
Sbjct: 574 LHGLMLHSRRKG*S*SRYFDQ*LRNLGRPISSQGFRLPL*CMTA 443
Score = 71.2 bits (149), Expect(2) = 2e-067
Identities = 29/43 (67%), Positives = 37/43 (86%)
Frame = +3 / -2
Query: 171 SWIDVAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDC 299
S IDVAFP++RL+I+++L PVA +LG NL+PGL VAIVMYDC
Sbjct: 572 SRIDVAFPQKRLIIVKVL*PVASELGEANLKPGLQVAIVMYDC 444
Score = 64.8 bits (135), Expect(2) = 7e-059
Identities = 28/44 (63%), Positives = 33/44 (75%)
Frame = -1 / +2
Query: 297 SHTLQWQHEALVGDWSTQVSELLVQVSGL*PIFPSGMQHQSMKE 166
SHTLQWQ EAL DW QV +LLV+V L* F +GMQHQS+K+
Sbjct: 446 SHTLQWQPEALA*DWPPQVPKLLVKVP*L*SAFSAGMQHQSVKQ 577
Score = 63.4 bits (132), Expect(2) = 9e-058
Identities = 27/44 (61%), Positives = 29/44 (65%)
Frame = +2 / -1
Query: 170 FMD*CCIPEGKIGHNPDTWTSSSETWVDQSPTRASCCHCNV*LQ 301
F D*CCIP K H+ T TSS TW QS RAS CHCNV*L+
Sbjct: 573 FTD*CCIPAEKADHSQGTLTSSFGTWGGQSQARASGCHCNV*LR 442
Score = 58.8 bits (122), Expect(2) = 4e-047
Identities = 29/43 (67%), Positives = 30/43 (69%)
Frame = -3 / +1
Query: 298 QSYITMAT*SPGWRLVYPSFGATGPSIWIMTNLSFGNATSIHE 170
QSYITMAT*SPG RL PS ATG S M +L GNATSI E
Sbjct: 445 QSYITMAT*SPGLRLASPSSEATGQSTLTMISLFCGNATSIRE 573
>gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 3', mRNA sequence
Length = 643
Score = 371 bits (804), Expect = e-102
Identities = 142/163 (87%), Positives = 151/163 (92%)
Frame = -2 / -3
Query: 659 SINMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQN 480
SI+MKGKVNGAVETCGESFHR+ RYLNFS+P+I K+FDP AC WAFGMNVFDLAEWRRQN
Sbjct: 641 SIDMKGKVNGAVETCGESFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQN 462
Query: 479 ITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHA 300
ITE+YH WQKLNED LWKLGTLP LVTFWNKTFPL RSWHVLGLGYNPHV+SRD+E A
Sbjct: 461 ITELYHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERA 282
Query: 299 AVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP* 171
AVIHYNGN+KPWLEIGLPKFRSYWSKYLDYDQ FLRECNINP*
Sbjct: 281 AVIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLRECNINP* 153
Score = 290 bits (627), Expect = 8e-078
Identities = 130/164 (79%), Positives = 135/164 (82%)
Frame = +1 / +1
Query: 169 LHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRP 348
LHGLMLHSRRK S*SRY DQ*LRNLGRPIS+QGF LPL*CMTAA S SLLLT GL*PRP
Sbjct: 151 LHGLMLHSRRKG*S*SRYFDQ*LRNLGRPISSQGFRLPL*CMTAARSRSLLLTCGL*PRP 330
Query: 349 RTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKA 528
RTCHDR RGNVLFQNVT+ GRVPSF S SSLSFC + * SVMFCRLHSARSNTFIPKA
Sbjct: 331 RTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFIPKA 510
Query: 529 QTQACGSKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPFMLID 660
Q A GSK I G EKF+ R RWKLSPQVSTAP TFPF+ ID
Sbjct: 511 QPHASGSKFFAITGLEKFK*RPKRWKLSPQVSTAPLTFPFISID 642
Score = 245 bits (529), Expect = 3e-064
Identities = 106/163 (65%), Positives = 130/163 (79%)
Frame = +3 / +3
Query: 171 SWIDVAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TK 350
S IDVAFP++RL+I+++L PVA +LG NL+PGL VAIVMYDC L VS M +VT*T+
Sbjct: 153 SRIDVAFPQKRLIIVKVL*PVASELGEANLKPGLQVAIVMYDCGTLQVSTAYMWVVT*TE 332
Query: 351 NMP*PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSP 530
NMP* EG+CLVPEC+QT RES KFP S+ +KLLP+V+ FSYVLPPPFSQ+KH+HSKSP
Sbjct: 333 NMP*SIEGECLVPECNQTWRESAKFP*QSVLVKLLPKVVEFSYVLPPPFSQIKHVHSKSP 512
Query: 531 NTSMWVKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD* 659
T + VK L +N *+IQV+ AME LSTSL ST+ LPLH+D*
Sbjct: 513 ATCIGVKILCNNRT*EIQVTAKAMEALSTSLYSTINLPLHID* 641
Score = 225 bits (485), Expect = 3e-058
Identities = 104/165 (63%), Positives = 120/165 (72%)
Frame = -1 / -2
Query: 660 VNQHEGEGKRCC*DLWREFPSL*SIPEFFKSHYHQEF*PTCLCLGFWNECV*PG*MEAAE 481
VN++EGEG CC DLWRE PSL + EF KS Y +EF*P C+ LGFWNE V* G*MEAAE
Sbjct: 642 VNRYEGEG*WCCRDLWRELPSLWPLLEFLKSCYCKEF*PRCMWLGFWNERV*SG*MEAAE 463
Query: 480 HN*NLSLVAEA**R*ITLETWYSPFVFGHILEQDISPQPVMACSWSRLQSPC*QQRH*AC 301
HN* L L+AEA* +ETW+SP G+ILEQDI P+ +MACS SRLQ C Q R AC
Sbjct: 462 HN*TLPLLAEA*RGRTAMETWHSPSRSGYILEQDIPPRSIMACSRSRLQPTCKQ*RPGAC 283
Query: 300 CSHTLQWQHEALVGDWSTQVSELLVQVSGL*PIFPSGMQHQSMKE 166
SHTLQWQ EAL DW QV +LLV+V L* F +GMQHQS+K+
Sbjct: 282 RSHTLQWQPEALA*DWPPQVPKLLVKVP*L*SAFSAGMQHQSVKQ 148
Score = 220 bits (475), Expect = 7e-057
Identities = 103/164 (62%), Positives = 112/164 (68%)
Frame = +2 / +2
Query: 170 FMD*CCIPEGKIGHNPDTWTSSSETWVDQSPTRASCCHCNV*LQHAQCLCC*HGDCNLDQ 349
F D*CCIP K H+ T TSS TW QS RAS CHCNV*L+HA L C H CNLD+
Sbjct: 152 FTD*CCIPAEKADHSQGTLTSSFGTWGGQSQARASGCHCNV*LRHAPGLYCLHVGCNLDR 331
Query: 350 EHAMTG*GEMSCSRM*PNTKGEYQVSRVIYLH*ASATSDKFQLCSAASIQPGQTHSFQKP 529
EHAM G MSCSRM*P+ +GE QVS + *ASA S + QLCSAASIQP QT SFQKP
Sbjct: 332 EHAMIDRGGMSCSRM*PDLEGECQVSIAVRPR*ASAKSGRVQLCSAASIQPDQTRSFQKP 511
Query: 530 KHKHVGQNSW**WDLKNSGIDHSDGNSLHKSQQHRLPSPSC*LT 661
H H GQNS * DL+NS SDG+SLHKS QH PSPS LT
Sbjct: 512 SHMHRGQNSLQ*QDLRNSSNGQSDGSSLHKSLQHH*PSPSYRLT 643
Score = 189 bits (407), Expect = 2e-047
Identities = 99/164 (60%), Positives = 105/164 (64%)
Frame = -3 / -1
Query: 661 GQST*RGXXXXXXXXXXXVSIAMIDT*IFQIPLSPRVLTHMLVFGLLE*MCLTWLNGGGR 482
GQS *RG SIA+ T*I Q+ L R+LT M V GLLE* CL WLNGGGR
Sbjct: 643 GQSI*RGRLMVL*RLVERASIALAVT*ISQVLLLQRILTPMHVAGLLE*TCLIWLNGGGR 464
Query: 481 T*LKFITRGRSLMKIDHSGNLVLSLCVWSHSGTRHFPSAGHGMFLV*VTIPMLTAETLSM 302
T*L T GRSL + D GNL LSL VW HSGTRH PS HGMF V*VT M ET S+
Sbjct: 463 T*LNSTTFGRSLTRTDCYGNLALSLQVWLHSGTRHSPSIDHGMFSV*VTTHM*AVETWSV 284
Query: 301 LQSYITMAT*SPGWRLVYPSFGATGPSIWIMTNLSFGNATSIHE 170
QSYITMAT*SPG RL PS ATG S M +L GNATSI E
Sbjct: 283 PQSYITMAT*SPGLRLASPSSEATGQSTLTMISLFCGNATSIRE 152
>gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. vulgare cDNA clone HX12J15
5-PRIME, mRNA sequence
Length = 653
Score = 369 bits (800), Expect = e-101
Identities = 141/162 (87%), Positives = 150/162 (92%)
Frame = -2 / -3
Query: 656 INMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNI 477
I+MKGKVNGAVETCGESFHR+ RYLNFS+P+I K+FDP AC WAFGMNVFDLAEWRRQNI
Sbjct: 651 IDMKGKVNGAVETCGESFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNI 472
Query: 476 TEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAA 297
TE+YH WQKLNED LWKLGTLP LVTFWNKTFPL RSWHVLGLGYNPHV+SRD+E AA
Sbjct: 471 TELYHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAA 292
Query: 296 VIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP* 171
VIHYNGN+KPWLEIGLPKFRSYWSKYLDYDQ FLRECNINP*
Sbjct: 291 VIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLRECNINP* 166
Score = 287 bits (621), Expect = 5e-077
Identities = 129/163 (79%), Positives = 134/163 (82%)
Frame = +1 / +2
Query: 169 LHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRP 348
LHGLMLHSRRK S*SRY DQ*LRNLGRPIS+QGF LPL*CMTAA S SLLLT GL*PRP
Sbjct: 164 LHGLMLHSRRKG*S*SRYFDQ*LRNLGRPISSQGFRLPL*CMTAARSRSLLLTCGL*PRP 343
Query: 349 RTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKA 528
RTCHDR RGNVLFQNVT+ GRVPSF S SSLSFC + * SVMFCRLHSARSNTFIPKA
Sbjct: 344 RTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFIPKA 523
Query: 529 QTQACGSKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPFMLI 657
Q A GSK I G EKF+ R RWKLSPQVSTAP TFPF+ I
Sbjct: 524 QPHASGSKFFAITGLEKFK*RPKRWKLSPQVSTAPLTFPFISI 652
Score = 244 bits (528), Expect = 4e-064
Identities = 105/162 (64%), Positives = 129/162 (79%)
Frame = +3 / +1
Query: 171 SWIDVAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TK 350
S IDVAFP++RL+I+++L PVA +LG NL+PGL VAIVMYDC L VS M +VT*T+
Sbjct: 166 SRIDVAFPQKRLIIVKVL*PVASELGEANLKPGLQVAIVMYDCGTLQVSTAYMWVVT*TE 345
Query: 351 NMP*PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSP 530
NMP* EG+CLVPEC+QT RES KFP S+ +KLLP+V+ FSYVLPPPFSQ+KH+HSKSP
Sbjct: 346 NMP*SIEGECLVPECNQTWRESAKFP*QSVLVKLLPKVVEFSYVLPPPFSQIKHVHSKSP 525
Query: 531 NTSMWVKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD 656
T + VK L +N *+IQV+ AME LSTSL ST+ LPLH+D
Sbjct: 526 ATCIGVKILCNNRT*EIQVTAKAMEALSTSLYSTINLPLHID 651
Score = 223 bits (481), Expect = 1e-057
Identities = 103/164 (62%), Positives = 119/164 (72%)
Frame = -1 / -2
Query: 657 NQHEGEGKRCC*DLWREFPSL*SIPEFFKSHYHQEF*PTCLCLGFWNECV*PG*MEAAEH 478
N++EGEG CC DLWRE PSL + EF KS Y +EF*P C+ LGFWNE V* G*MEAAEH
Sbjct: 652 NRYEGEG*WCCRDLWRELPSLWPLLEFLKSCYCKEF*PRCMWLGFWNERV*SG*MEAAEH 473
Query: 477 N*NLSLVAEA**R*ITLETWYSPFVFGHILEQDISPQPVMACSWSRLQSPC*QQRH*ACC 298
N* L L+AEA* +ETW+SP G+ILEQDI P+ +MACS SRLQ C Q R AC
Sbjct: 472 N*TLPLLAEA*RGRTAMETWHSPSRSGYILEQDIPPRSIMACSRSRLQPTCKQ*RPGACR 293
Query: 297 SHTLQWQHEALVGDWSTQVSELLVQVSGL*PIFPSGMQHQSMKE 166
SHTLQWQ EAL DW QV +LLV+V L* F +GMQHQS+K+
Sbjct: 292 SHTLQWQPEALA*DWPPQVPKLLVKVP*L*SAFSAGMQHQSVKQ 161
Score = 219 bits (472), Expect = 2e-056
Identities = 101/160 (63%), Positives = 110/160 (68%)
Frame = +2 / +3
Query: 170 FMD*CCIPEGKIGHNPDTWTSSSETWVDQSPTRASCCHCNV*LQHAQCLCC*HGDCNLDQ 349
F D*CCIP K H+ T TSS TW QS RAS CHCNV*L+HA L C H CNLD+
Sbjct: 165 FTD*CCIPAEKADHSQGTLTSSFGTWGGQSQARASGCHCNV*LRHAPGLYCLHVGCNLDR 344
Query: 350 EHAMTG*GEMSCSRM*PNTKGEYQVSRVIYLH*ASATSDKFQLCSAASIQPGQTHSFQKP 529
EHAM G MSCSRM*P+ +GE QVS + *ASA S + QLCSAASIQP QT SFQKP
Sbjct: 345 EHAMIDRGGMSCSRM*PDLEGECQVSIAVRPR*ASAKSGRVQLCSAASIQPDQTRSFQKP 524
Query: 530 KHKHVGQNSW**WDLKNSGIDHSDGNSLHKSQQHRLPSPS 649
H H GQNS * DL+NS SDG+SLHKS QH PSPS
Sbjct: 525 SHMHRGQNSLQ*QDLRNSSNGQSDGSSLHKSLQHH*PSPS 644
Score = 186 bits (401), Expect = 1e-046
Identities = 98/163 (60%), Positives = 104/163 (63%)
Frame = -3 / -1
Query: 658 QST*RGXXXXXXXXXXXVSIAMIDT*IFQIPLSPRVLTHMLVFGLLE*MCLTWLNGGGRT 479
QS *RG SIA+ T*I Q+ L R+LT M V GLLE* CL WLNGGGRT
Sbjct: 653 QSI*RGRLMVL*RLVERASIALAVT*ISQVLLLQRILTPMHVAGLLE*TCLIWLNGGGRT 474
Query: 478 *LKFITRGRSLMKIDHSGNLVLSLCVWSHSGTRHFPSAGHGMFLV*VTIPMLTAETLSML 299
*L T GRSL + D GNL LSL VW HSGTRH PS HGMF V*VT M ET S+
Sbjct: 473 *LNSTTFGRSLTRTDCYGNLALSLQVWLHSGTRHSPSIDHGMFSV*VTTHM*AVETWSVP 294
Query: 298 QSYITMAT*SPGWRLVYPSFGATGPSIWIMTNLSFGNATSIHE 170
QSYITMAT*SPG RL PS ATG S M +L GNATSI E
Sbjct: 293 QSYITMAT*SPGLRLASPSSEATGQSTLTMISLFCGNATSIRE 165
>gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0012C17f, mRNA sequence
Length = 785
Score = 337 bits (730), Expect(3) = 5e-097
Identities = 129/157 (82%), Positives = 143/157 (91%)
Frame = -2 / +1
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLPE++PKLDKMVFLD D+VV+KD+ GLWSI+MKGKVNGAVETCGE
Sbjct: 121 RNPKYLSMLNHLRFYLPEIYPKLDKMVFLDGDVVVRKDITGLWSIDMKGKVNGAVETCGE 300
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
SFHR+ RYLNFS+P+I K+FDP AC WAFGMNVFDLAEWRRQNITE+YH WQKLNED L
Sbjct: 301 SFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLL 480
Query: 428 WKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNS 318
WKLGTLP LVTFWNKTFPL RSWHVLGLGYNPHV+S
Sbjct: 481 WKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSS 591
Score = 242 bits (523), Expect(2) = 1e-065
Identities = 108/141 (76%), Positives = 112/141 (79%)
Frame = +1 / -1
Query: 319 LLTWGL*PRPRTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHS 498
LLT GL*PRPRTCHDR RGNVLFQNVT+ GRVPSF S SSLSFC + * SVMFCRLHS
Sbjct: 590 LLTCGL*PRPRTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHS 411
Query: 499 ARSNTFIPKAQTQACGSKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPFMLIDQRPARS 678
ARSNTFIPKAQ A GSK I G EKF+ R RWKLSPQVSTAP TFPF+ IDQRP S
Sbjct: 410 ARSNTFIPKAQPHASGSKFFAITGLEKFK*RPKRWKLSPQVSTAPLTFPFISIDQRPVMS 231
Query: 679 FFTTMSSSRKTILSSFGCISG 741
F TT S SRKTILSSFG ISG
Sbjct: 230 FLTTTSPSRKTILSSFG*ISG 168
Score = 227 bits (491), Expect = 5e-059
Identities = 96/151 (63%), Positives = 124/151 (82%)
Frame = +3 / -3
Query: 324 NMGIVT*TKNMP*PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQ 503
NM +VT*T+NMP* EG+CLVPEC+QT RES KFP S+ +KLLP+V+ FSYVLPPPFSQ
Sbjct: 585 NMWVVT*TENMP*SIEGECLVPECNQTWRESAKFP*QSVLVKLLPKVVEFSYVLPPPFSQ 406
Query: 504 VKHIHSKSPNTSMWVKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD*PKASQILL 683
+KH+HSKSP T + VK L +N *+IQV+ AME LSTSL ST+ LPLH+D*P+AS +L
Sbjct: 405 IKHVHSKSPATCIGVKILCNNRT*EIQVTAKAMEALSTSLYSTINLPLHID*PEASYVLS 226
Query: 684 YYDVIIKKDHLVKLWMHLR*VKAEMIED*QV 776
YY + IKKDHLV+LW++LR V+++MI+ *++
Sbjct: 225 YYYIAIKKDHLVELWINLRQVESQMIKH*KI 133
Score = 204 bits (439), Expect = 7e-052
Identities = 96/153 (62%), Positives = 106/153 (69%)
Frame = +2 / -2
Query: 320 C*HGDCNLDQEHAMTG*GEMSCSRM*PNTKGEYQVSRVIYLH*ASATSDKFQLCSAASIQ 499
C*H CNLD+EHAM G MSCSRM*P+ +GE QVS + *ASA S + QLCSAASIQ
Sbjct: 589 C*HVGCNLDREHAMIDRGGMSCSRM*PDLEGECQVSIAVRPR*ASAKSGRVQLCSAASIQ 410
Query: 500 PGQTHSFQKPKHKHVGQNSW**WDLKNSGIDHSDGNSLHKSQQHRLPSPSC*LTKGQPDP 679
P QT SFQKP H H GQNS * DL+NS SDG+SLHKS QH PSPS LT+GQ P
Sbjct: 409 PDQTRSFQKPSHMHRGQNSLQ*QDLRNSSNGQSDGSSLHKSLQHH*PSPSYRLTRGQLCP 230
Query: 680 SLLRCHHQERPSCQALDASQVGKSGDD*GLTGT 778
LL HQERPSC+ALD SQ + DD* L T
Sbjct: 229 FLLLHRHQERPSCRALDKSQASRISDD*ALKDT 131
Score = 201 bits (434), Expect = 3e-051
Identities = 98/160 (61%), Positives = 112/160 (70%)
Frame = -1 / +3
Query: 798 FERPPGQVPVNPQSSPLLPT*DASKA*QDGLS***HRSKEGSGWPLVNQHEGEGKRCC*D 619
FE +V N SS +L *D SKA QDGLS* SK+G WPLVN++EGEG CC D
Sbjct: 111 FEVQKPKVSFNA*SSEILLA*DLSKARQDGLS*WRCSSKKGHNWPLVNRYEGEG*WCCRD 290
Query: 618 LWREFPSL*SIPEFFKSHYHQEF*PTCLCLGFWNECV*PG*MEAAEHN*NLSLVAEA**R 439
LWRE PSL + EF KS Y +EF*P C+ LGFWNE V* G*MEAAEHN* L L+AEA*
Sbjct: 291 LWRELPSLWPLLEFLKSCYCKEF*PRCMWLGFWNERV*SG*MEAAEHN*TLPLLAEA*RG 470
Query: 438 *ITLETWYSPFVFGHILEQDISPQPVMACSWSRLQSPC*Q 319
+ETW+SP G+ILEQDI P+ +MACS SRLQ C*Q
Sbjct: 471 RTAMETWHSPSRSGYILEQDIPPRSIMACSRSRLQPTC*Q 590
Score = 164 bits (352), Expect = 7e-040
Identities = 90/154 (58%), Positives = 94/154 (61%)
Frame = -3 / +2
Query: 769 QSSIISAFTYLRCIQSLTRWSFLMMTS**RRIWLAFGQST*RGXXXXXXXXXXXVSIAMI 590
Q II T LR IQS TRWSFLM ** R LA GQS *RG SIA+
Sbjct: 140 QCLII*DSTCLRFIQSSTRWSFLMAM***ERT*LASGQSI*RGRLMVL*RLVERASIALA 319
Query: 589 DT*IFQIPLSPRVLTHMLVFGLLE*MCLTWLNGGGRT*LKFITRGRSLMKIDHSGNLVLS 410
T*I Q+ L R+LT M V GLLE* CL WLNGGGRT*L T GRSL + D GNL LS
Sbjct: 320 VT*ISQVLLLQRILTPMHVAGLLE*TCLIWLNGGGRT*LNSTTFGRSLTRTDCYGNLALS 499
Query: 409 LCVWSHSGTRHFPSAGHGMFLV*VTIPMLTAETL 308
L VW HSGTRH PS HGMF V*VT ML L
Sbjct: 500 LQVWLHSGTRHSPSIDHGMFSV*VTTHMLAVRNL 601
Score = 29.9 bits (59), Expect(3) = 5e-097
Identities = 9/14 (64%), Positives = 13/14 (92%)
Frame = -2 / +2
Query: 239 RSYWSKYLDYDQSF 198
++ WSKYLD+DQ+F
Sbjct: 674 KASWSKYLDFDQAF 715
Score = 27.6 bits (54), Expect(2) = 1e-065
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +1 / -2
Query: 241 NLGRPISNQGFMLPL 285
NLGRP +QGF +P+
Sbjct: 670 NLGRPFPSQGFRVPM 626
Score = 27.2 bits (53), Expect(3) = 5e-097
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -2 / +3
Query: 281 GNMKPWLEIGLPKF 240
G + PWL GLPKF
Sbjct: 630 GTLNPWLGNGLPKF 671
>gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 5', mRNA sequence
Length = 598
Score = 344 bits (745), Expect = 4e-094
Identities = 124/188 (65%), Positives = 159/188 (84%)
Frame = -2 / +2
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLP+++PKLDK++FLDDDIVV+KDL GLW +++ G VNGAV TCGE
Sbjct: 35 RNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNGAVFTCGE 214
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
SFHR+D+YLNFSNP I ++FDP+AC WA+GMN+FDL +W+ ++IT IYH WQ +NEDR L
Sbjct: 215 SFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVL 394
Query: 428 WKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGL 249
WKLGTLP L+TF+ T PL +SWHVLGLGYNP ++ +I++AAV HYNGNMKPWLE+ +
Sbjct: 395 WKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAM 574
Query: 248 PKFRSYWS 225
K+R YW+
Sbjct: 575 TKYRPYWT 598
Score = 189 bits (408), Expect = 1e-047
Identities = 93/161 (57%), Positives = 102/161 (63%)
Frame = +1 / -2
Query: 259 SNQGFMLPL*CMTAACSMSLLLTWGL*PRPRTCHDRLRGNVLFQNVTKHKGRVPSFQSDL 438
S+ GFM PL* TAA S+S GL P PRTCHD RG V NV + G VPSFQ L
Sbjct: 564 SSHGFMFPL**ATAALSISERSMLGLYPSPRTCHDLSRGCVSL*NVMRPGGSVPSFQRTL 385
Query: 439 SSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSKLLVIMGFEKFRYRS*RWKLSPQ 618
SS FCH *I V+ H RS FIP AQ QA GSK + GFEK RY S RWKLSPQ
Sbjct: 384 SSFMFCHLW*IPVISLFFHCFRSKMFIP*AQPQALGSKFRAMCGFEKLRYLSKRWKLSPQ 205
Query: 619 VSTAPFTFPFMLIDQRPARSFFTTMSSSRKTILSSFGCISG 741
V+TAP T PF P +SF TTMSSSRK+ILS+FG I G
Sbjct: 204 VNTAPLTIPFRSTSHNPVKSFCTTMSSSRKSILSNFG*ICG 82
Score = 120 bits (257), Expect = 9e-027
Identities = 61/183 (33%), Positives = 112/183 (61%)
Frame = +3 / -1
Query: 228 PVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP*PAEGKCLVPECDQTQ 407
PV LG LQP LHV IVM D S++ + AV+ +V+*+K+MP +G + E + +
Sbjct: 595 PVWSVLGHCQLQPWLHVPIVMSDSSIVYL*AVDAWVVS*SKDMPRFIKGVRQLVERHEAR 416
Query: 408 RESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSMWVKTLGDNGI*KIQV 587
+ + P+ +FI +LP V++ + P Q+K +H SP TS+ +K + I*+++V
Sbjct: 415 WKCPELPKNPVFIHVLPLVVDPGNIFVLPLLQIKDVHPISPTTSIRIKVPSNVWI*EVKV 236
Query: 588 SIIAMETLSTSLNSTVYLPLHVD*PKASQILLYYDVIIKKDHLVKLWMHLR*VKAEMIED 767
+ +ETLST + T+ + ++ P++ QI L+ VII++ +L++LW+ L +K+++++
Sbjct: 235 LVETVETLSTGKHCTIDHSIQINIPQSCQIFLHNYVIIEEKYLIQLWIDLWEIKSQVVQH 56
Query: 768 *QV 776
+V
Sbjct: 55 GEV 47
>gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10L09
5-PRIME, mRNA sequence
Length = 684
Score = 325 bits (705), Expect = 1e-088
Identities = 120/175 (68%), Positives = 151/175 (86%)
Frame = -2 / +3
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLP+++PKL+K++FLDDDIVV+KDL GLW +++ G VNGAVETCGE
Sbjct: 159 RNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQKDLTGLWEVDLNGNVNGAVETCGE 338
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
SFHR+D+YLNFSNP I+++FDP+AC WA+GMN+FDL EW++++IT IYH WQ +NE+R L
Sbjct: 339 SFHRFDKYLNFSNPNISQNFDPNACGWAYGMNMFDLEEWKKKDITGIYHKWQNMNENRLL 518
Query: 428 WKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPW 264
WKLGTLP L+TF+ T PL +SWHVLGLGYNP V +I+ AAVIHYNGNMKPW
Sbjct: 519 WKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPTVEHAEIDTAAVIHYNGNMKPW 683
Score = 188 bits (405), Expect = 3e-047
Identities = 91/154 (59%), Positives = 97/154 (62%)
Frame = +1 / -3
Query: 268 GFMLPL*CMTAACSMSLLLTWGL*PRPRTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSL 447
GFM PL* MTAA S+S T GL P P TCHD RG V V + G VPSF S L S
Sbjct: 679 GFMFPL*WMTAAVSISACSTVGLYPNPSTCHDLSRGCVSL*KVMRPGGSVPSFHSSLFSF 500
Query: 448 SFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSKLLVIMGFEKFRYRS*RWKLSPQVST 627
FCH *I VM HS+RSN FIP AQ A GSK I GFEK RY S RWKLSP VST
Sbjct: 499 MFCHLW*IPVMSFFFHSSRSNIFIP*AQPHALGSKFCEIFGFEKLRYLSNRWKLSPHVST 320
Query: 628 APFTFPFMLIDQRPARSFFTTMSSSRKTILSSFG 729
APFTFP P +SF+TTMSSSRK IL + G
Sbjct: 319 APFTFPLRSTSHSPVKSFWTTMSSSRKRILFNLG 218
Score = 134 bits (288), Expect = 5e-031
Identities = 68/171 (39%), Positives = 113/171 (66%)
Frame = +3 / -2
Query: 264 PGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP*PAEGKCLVPECDQTQRESTKFPE*SIF 443
P LHV++V+ D +++ ++ +V *+K+MP* +G + E +T RE +FP+ +F
Sbjct: 683 PWLHVSVVVDDGGGVDLRVLDSWVVP*SKHMP*FVQGVRQLVESHETWRECPQFPQQPVF 504
Query: 444 IKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSMWVKTLGDNGI*KIQVSIIAMETLSTSL 623
I +LP +++ S VL PF Q+KHIHS SP T + +K L + +*KI+V + ++ETL T
Sbjct: 503 IHVLPLMVDPSNVLLLPFFQIKHIHSISPTTCIRIKIL*NIWV*KIEVLVKSVETLPTCF 324
Query: 624 NSTVYLPLHVD*PKASQILLYYDVIIKKDHLVKLWMHLR*VKAEMIED*QV 776
STVY+P+ ++ P +SQILL ++VII++ ++L + L VK+ +IE Q+
Sbjct: 323 YSTVYIPIKINLP*SSQILLDHNVIIEEKDFIQLRIDLWEVKS*VIEHGQI 171
Score = 107 bits (228), Expect = 9e-023
Identities = 53/125 (42%), Positives = 69/125 (55%)
Frame = -1 / +2
Query: 699 **HRSKEGSGWPLVNQHEGEGKRCC*DLWREFPSL*SIPEFFKSHYHQEF*PTCLCLGFW 520
**H +G W + E KRCC ++W EFP +* +P+FFK Y EF* C+ LG W
Sbjct: 248 **HCGPKGFDWTMGG*S*WECKRCCRNMWGEFPPI*QVPQFFKPKYFTEF*S*CMWLGLW 427
Query: 519 NECV*PG*MEAAEHN*NLSLVAEA**R*ITLETWYSPFVFGHILEQDISPQPVMACSWSR 340
NE V* G ME H +L VAE *+ +ET +P L+ D P +MAC+W R
Sbjct: 428 NEYV*SGRMEEEGHYWDLP*VAEHE*KQAAVETGDTPARSHDFLQADAPPGQIMACAWIR 607
Query: 339 LQSPC 325
+Q C
Sbjct: 608 VQPNC 622
>gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. vulgare cDNA clone HX14D07
5-PRIME, mRNA sequence
Length = 529
Score = 305 bits (661), Expect = 2e-082
Identities = 111/176 (63%), Positives = 143/176 (81%)
Frame = -2 / +2
Query: 713 MVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFDPHAC 534
++FLDDD+VV++DL GLW I+M GKVNGAVETC SFHRY +Y+NFS+P+I F+P+AC
Sbjct: 2 ILFLDDDVVVQRDLTGLWKIDMDGKVNGAVETCFGSFHRYWQYMNFSHPLIKAKFNPNAC 181
Query: 533 VWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWH 354
WA+GMN FDL WRR+ TE YH WQ NE+R LWKLGTLP L+TF++ T PL +SWH
Sbjct: 182 GWAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWH 361
Query: 353 VLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLREC 186
VLGLGYNP ++ +I +AAV+H+NGNMKPWL+IG+ +FR W+KY+DYD SF+R+C
Sbjct: 362 VLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 529
Score = 144 bits (309), Expect = 6e-034
Identities = 84/176 (47%), Positives = 93/176 (52%)
Frame = +1 / -2
Query: 187 HSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRPRTCHDR 366
H R + S*S Y Q N PIS+QGF+ PL C TAA +S +L GL PRPRTC D
Sbjct: 528 H*RMNESS*STYFVQSCLNWFIPISSQGFIFPLKCTTAAFLISSMLMLGLYPRPRTCQDL 349
Query: 367 LRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACG 546
RG V+ NV G VP+F S S C SV F L RS F+P AQ A G
Sbjct: 348 SRGFVVE*NVINPGGNVPNFHSSRFSF*VCQ*WYCSVDFSLLQELRSKKFMP*AQPHALG 169
Query: 547 SKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPFMLIDQRPARSFFTTMSSSRKTI 714
+I G EKF Y R VSTAPFTFP M I P RS TT SSSRK I
Sbjct: 168 LNFALIRGCEKFIYCQYR*NDPKHVSTAPFTFPSMSIFHNPVRSRCTTTSSSRKRI 1
Score = 118 bits (252), Expect = 4e-026
Identities = 56/174 (32%), Positives = 100/174 (57%)
Frame = +3 / -1
Query: 186 AFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP*P 365
A ER+VII + P P+L TN+QP LH+ I M++ S+ N+ + I+ T++MP
Sbjct: 529 ALTNERVVIIDVFCPELPELVHTNIQPRLHIPIEMHNSSVSNLLHADAWIIPETQDMPRF 350
Query: 366 AEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSMW 545
+ C ECD + + ++ P * + I LP ++ LPPP ++K +H+ SP T +
Sbjct: 349 VQRLCGRVECD*SWGQCSQLP**PVLILSLPVMVLLGRFLPPPRIKIKEVHAISPATCIR 170
Query: 546 VKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD*PKASQILLYYDVIIKK 707
++ D G+ ++ + I M+ +++LP+HV+ P++ +I LY D+II++
Sbjct: 169 IELCFDKGVREVHILPIPMKRPKACFYCSIHLPIHVNLPQSCEIPLYDDIIIQE 8
>gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010D065 5-PRIME, mRNA sequence
Length = 786
Score = 299 bits (648), Expect = 1e-080
Identities = 104/168 (61%), Positives = 137/168 (81%)
Frame = -2 / +1
Query: 680 KDLAGLWSINMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDL 501
KDL GLW +++ G VNGAV TCGESFHR+D+YLNFSNP I ++FDP+AC WA+GMN+FDL
Sbjct: 16 KDLTGLWDVDLNGMVNGAVFTCGESFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDL 195
Query: 500 AEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVN 321
+W+ ++IT IYH WQ +NEDR LWKLGTLP L+TF+ T PL +SWHVLGLGYNP ++
Sbjct: 196 KQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSID 375
Query: 320 SRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNIN 177
+I++AAV HYNGNMKPWLE+ + K+R YW++Y+ YD ++R CN++
Sbjct: 376 RSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNLS 519
Score = 171 bits (368), Expect = 4e-042
Identities = 89/171 (52%), Positives = 98/171 (57%)
Frame = +1 / -2
Query: 169 LHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRP 348
++ L LH R WS YL Q R L S+ GFM PL* TAA S+S GL P P
Sbjct: 527 IYSLRLHPRM*GWSYFIYLVQYGRYLVIASSSHGFMFPL**ATAALSISERSMLGLYPSP 348
Query: 349 RTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKA 528
RTCHD RG V NV + G VPSFQ LSS FCH *I V+ H RS FIP A
Sbjct: 347 RTCHDLSRGCVSL*NVMRPGGSVPSFQRTLSSFMFCHLW*IPVISLFFHCFRSKMFIP*A 168
Query: 529 QTQACGSKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPFMLIDQRPARSF 681
Q QA GSK + GFEK RY S RWKLSPQV+TAP T PF P +SF
Sbjct: 167 QPQALGSKFRAMCGFEKLRYLSKRWKLSPQVNTAPLTIPFRSTSHNPVKSF 15
Score = 102 bits (218), Expect = 2e-021
Identities = 57/165 (34%), Positives = 96/165 (58%)
Frame = +3 / -1
Query: 183 VAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP* 362
VA R+VI+ I PV LG LQP LHV IVM D S++ + AV+ +V+*+K+MP
Sbjct: 513 VASTDVRVVILYIPCPVWSVLGHCQLQPWLHVPIVMSDSSIVYL*AVDAWVVS*SKDMPR 334
Query: 363 PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSM 542
+G + E + + + + P+ +FI +LP V++ + P Q+K +H SP TS+
Sbjct: 333 FIKGVRQLVERHEARWKCPELPKNPVFIHVLPLVVDPGNIFVLPLLQIKDVHPISPTTSI 154
Query: 543 WVKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD*PKASQI 677
+K + I*+++V + +ETLST + T+ + ++ P++ QI
Sbjct: 153 RIKVPSNVWI*EVKVLVETVETLSTGKHCTIDHSIQINIPQSCQI 19
>gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 5', mRNA sequence
Length = 630
Score = 283 bits (613), Expect = 7e-076
Identities = 102/157 (64%), Positives = 130/157 (82%)
Frame = -2 / +2
Query: 656 INMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNI 477
+++KG VNGAVETC ESFHR+D YLNFS+P I+++FDP AC WAFGMN+FDL EW+++NI
Sbjct: 11 VDLKGMVNGAVETCKESFHRFDTYLNFSHPKISENFDPRACGWAFGMNMFDLKEWKKRNI 190
Query: 476 TEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAA 297
T IYH WQ LNEDR LWKLGTLP L+TF+N T PL +WHVLGLGY+P V+ +IE+AA
Sbjct: 191 TGIYHYWQNLNEDRKLWKLGTLPPGLITFYNLTHPLDHTWHVLGLGYDPAVDIAEIENAA 370
Query: 296 VIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLREC 186
V+HYNGN KPWL++ + K+++YWSKY+D D S +R C
Sbjct: 371 VVHYNGNYKPWLDLAISKYKAYWSKYVDVDSSHIRHC 481
Score = 141 bits (303), Expect = 4e-033
Identities = 81/151 (53%), Positives = 88/151 (58%)
Frame = +1 / -1
Query: 193 RRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRPRTCHDRLR 372
R + S S YLDQ* L S+QG PL* TAA S+S + T G *PRP+TC
Sbjct: 474 RMCELSTSTYLDQ*ALYLEMARSSQGL*FPL**TTAAFSISAMSTAGS*PRPKTCQV*SN 295
Query: 373 GNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSK 552
G V NV G VPSF + SS FC I VMF HS RSN FIPKAQ QA GSK
Sbjct: 294 GCVKL*NVISPGGNVPSFHNLRSSFKFCQ*WYIPVMFRFFHSFRSNMFIPKAQPQARGSK 115
Query: 553 LLVIMGFEKFRYRS*RWKLSPQVSTAPFTFP 645
I+G EK R S RWKLS QVSTAPFT P
Sbjct: 114 FSEILGCEKLR*VSKRWKLSLQVSTAPFTIP 22
Score = 127 bits (272), Expect = 7e-029
Identities = 63/160 (39%), Positives = 94/160 (58%)
Frame = +3 / -3
Query: 183 VAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP* 362
VA P V I ILGPV LG +QPGL + IV+ +L+ S VN I+ ++MP
Sbjct: 484 VAMPNV*AVNIDILGPVGLVLGNGKVQPGLVIPIVVNYRCILDFSNVNSRIIAKAQDMPS 305
Query: 363 PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSM 542
+ C + EC+ + R + P+ +I I++LP ++ S V P QVKH+HSKSP TS
Sbjct: 304 VIQWVCQIVECN*SWR*CAQLPQLAILIQILPIMVYSSDVSLLPLLQVKHVHSKSPTTST 125
Query: 543 WVKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD*P 662
W+K L D +*++++S+ AMET +ST+Y P ++ P
Sbjct: 124 WIKVLRDLRM*EVEISVEAMETFFAGFDSTIYHPFKINIP 5
>gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 3', mRNA sequence
Length = 565
Score = 283 bits (613), Expect = 7e-076
Identities = 102/157 (64%), Positives = 130/157 (82%)
Frame = -2 / -1
Query: 656 INMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNI 477
+++KG VNGAVETC ESFHR+D YLNFS+P I+++FDP AC WAFGMN+FDL EW+++NI
Sbjct: 559 VDLKGMVNGAVETCKESFHRFDTYLNFSHPKISENFDPRACGWAFGMNMFDLKEWKKRNI 380
Query: 476 TEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAA 297
T IYH WQ LNEDR LWKLGTLP L+TF+N T PL +WHVLGLGY+P V+ +IE+AA
Sbjct: 379 TGIYHYWQNLNEDRKLWKLGTLPPGLITFYNLTHPLDHTWHVLGLGYDPAVDIAEIENAA 200
Query: 296 VIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLREC 186
V+HYNGN KPWL++ + K+++YWSKY+D D S +R C
Sbjct: 199 VVHYNGNYKPWLDLAISKYKAYWSKYVDVDSSHIRHC 89
Score = 141 bits (303), Expect = 4e-033
Identities = 81/151 (53%), Positives = 88/151 (58%)
Frame = +1 / +3
Query: 193 RRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRPRTCHDRLR 372
R + S S YLDQ* L S+QG PL* TAA S+S + T G *PRP+TC
Sbjct: 96 RMCELSTSTYLDQ*ALYLEMARSSQGL*FPL**TTAAFSISAMSTAGS*PRPKTCQV*SN 275
Query: 373 GNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSK 552
G V NV G VPSF + SS FC I VMF HS RSN FIPKAQ QA GSK
Sbjct: 276 GCVKL*NVISPGGNVPSFHNLRSSFKFCQ*WYIPVMFRFFHSFRSNMFIPKAQPQARGSK 455
Query: 553 LLVIMGFEKFRYRS*RWKLSPQVSTAPFTFP 645
I+G EK R S RWKLS QVSTAPFT P
Sbjct: 456 FSEILGCEKLR*VSKRWKLSLQVSTAPFTIP 548
Score = 127 bits (272), Expect = 7e-029
Identities = 63/160 (39%), Positives = 94/160 (58%)
Frame = +3 / +2
Query: 183 VAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP* 362
VA P V I ILGPV LG +QPGL + IV+ +L+ S VN I+ ++MP
Sbjct: 86 VAMPNV*AVNIDILGPVGLVLGNGKVQPGLVIPIVVNYRCILDFSNVNSRIIAKAQDMPS 265
Query: 363 PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSM 542
+ C + EC+ + R + P+ +I I++LP ++ S V P QVKH+HSKSP TS
Sbjct: 266 VIQWVCQIVECN*SWR*CAQLPQLAILIQILPIMVYSSDVSLLPLLQVKHVHSKSPTTST 445
Query: 543 WVKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD*P 662
W+K L D +*++++S+ AMET +ST+Y P ++ P
Sbjct: 446 WIKVLRDLRM*EVEISVEAMETFFAGFDSTIYHPFKINIP 565
>gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B7 5' similar to 68 kDa
protein, mRNA sequence
Length = 616
Score = 283 bits (612), Expect = 9e-076
Identities = 98/160 (61%), Positives = 131/160 (81%)
Frame = -2 / +1
Query: 656 INMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNI 477
+++ G VNGAV TCGESFHR+D+YLNFSNP I ++FDP+AC WA+GMN+FDL +W+ ++I
Sbjct: 1 VDLNGMVNGAVFTCGESFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDI 180
Query: 476 TEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAA 297
T IYH WQ +NEDR LWKLGTLP L+TF+ T PL +SWHVLGLGYNP ++ +I++AA
Sbjct: 181 TGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAA 360
Query: 296 VIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNIN 177
V HYNGNMKPWLE+ + K+R YW++Y+ YD ++R CN++
Sbjct: 361 VAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNLS 480
Score = 164 bits (353), Expect = 5e-040
Identities = 86/160 (53%), Positives = 94/160 (58%)
Frame = +1 / -3
Query: 169 LHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRP 348
++ L LH R WS YL Q R L S+ GFM PL* TAA S+S GL P P
Sbjct: 488 IYSLRLHPRM*GWSYFIYLVQYGRYLVIASSSHGFMFPL**ATAALSISERSMLGLYPSP 309
Query: 349 RTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKA 528
RTCHD RG V NV + G VPSFQ LSS FCH *I V+ H RS FIP A
Sbjct: 308 RTCHDLSRGCVSL*NVMRPGGSVPSFQRTLSSFMFCHLW*IPVISLFFHCFRSKMFIP*A 129
Query: 529 QTQACGSKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPF 648
Q QA GSK + GFEK RY S RWKLSPQV+TAP T PF
Sbjct: 128 QPQALGSKFRAMCGFEKLRYLSKRWKLSPQVNTAPLTIPF 9
>gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0025O15f, mRNA sequence
Length = 674
Score = 259 bits (560), Expect = 1e-068
Identities = 97/180 (53%), Positives = 129/180 (71%)
Frame = -2 / +1
Query: 728 PKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSF 549
P L+K+VFLDDD+VV+KDL L+SI + G V GAVETC ESFHRY ++ FS PII+
Sbjct: 25 PNLEKVVFLDDDVVVQKDLTQLFSIELHGNVIGAVETCLESFHRYHKWGGFSQPIISSKI 204
Query: 548 DPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPL 369
DP+ C WAFGMN+FDL WR+ N T +YH WQ+ N D+ LW+ GTLP L+TF+ PL
Sbjct: 205 DPYTCGWAFGMNIFDLIAWRKANATSLYHYWQEQNADQLLWRTGTLPAGLLTFYGLMEPL 384
Query: 368 SRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRE 189
R WH+LGLGY+ ++ R IE AAV+HYNGNMKPWL++ + ++S W +++ +RE
Sbjct: 385 DRRWHLLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIHPYKSIWGADVNFSHPHIRE 564
Score = 71.6 bits (150), Expect(3) = 2e-023
Identities = 37/80 (46%), Positives = 45/80 (56%)
Frame = +1 / -1
Query: 340 PRPRTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFI 519
PRP CH R RG++ NV + G VP QS+ S+ C ** V F L + RSN FI
Sbjct: 413 PRPNRCHLRSRGSIRP*NVRRPAGSVPVLQSN*SAFCSCQ***SDVAFAFLQAIRSNIFI 234
Query: 520 PKAQTQACGSKLLVIMGFEK 579
PKA Q GS +I+G EK
Sbjct: 233 PKAHPQVYGSIFELIIGCEK 174
Score = 44.1 bits (90), Expect(3) = 2e-023
Identities = 21/42 (50%), Positives = 25/42 (59%)
Frame = +1 / -1
Query: 610 SPQVSTAPFTFPFMLIDQRPARSFFTTMSSSRKTILSSFGCI 735
S VSTAP T P +++ +SF TT SSSRKT S G I
Sbjct: 143 SKHVSTAPITLPCNSMEKSCVKSFCTTTSSSRKTTFSRLGSI 18
Score = 30.9 bits (61), Expect(3) = 2e-023
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +1 / -1
Query: 262 NQGFMLPL*CMTAACSMS 315
+QGFM PL*C TAA S++
Sbjct: 491 SQGFMFPL*CTTAALSIN 438
>gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ001_M07 5', mRNA sequence
Length = 512
Score = 242 bits (523), Expect = 2e-063
Identities = 93/151 (61%), Positives = 118/151 (78%)
Frame = -2 / +3
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFY+PE++P+L K+VFLDDDIVV+KDL+ L++IN+ G V GAVETC E
Sbjct: 36 RNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETCME 215
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
+FHR+ +YLN S+P+I FDP AC WAFGMNV DL EWR +N+T IYH WQ+ N D +L
Sbjct: 216 TFHRFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDLVEWRNKNVTGIYHYWQERNADHTL 395
Query: 428 WKLGTLPLCLVTFWNKTFPLSRSWHVLGLGY 336
WKLG+LP L+ F+ L+ WHVLGLGY
Sbjct: 396 WKLGSLPPGLLAFYGLVEALNPKWHVLGLGY 488
Score = 109 bits (233), Expect = 2e-023
Identities = 61/111 (54%), Positives = 66/111 (59%)
Frame = +1 / -2
Query: 409 GRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSKLLVIMGFEKFRY 588
G+ PSF S+L C * I V F LHS RS+TFIP AQ QA GSK I G E RY
Sbjct: 415 GKDPSFHRV*SALRSCQ**YIPVTFLFLHSTRSSTFIPNAQPQASGSK*ARIRG*E*LRY 236
Query: 589 RS*RWKLSPQVSTAPFTFPFMLIDQRPARSFFTTMSSSRKTILSSFGCISG 741
W +S QVSTAP T P LI + RSF TTMSSSR T L S G ISG
Sbjct: 235 L*NLWNVSMQVSTAPITLPLRLIVNKSDRSF*TTMSSSRNTTLCSSG*ISG 83
>gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 5', mRNA sequence
Length = 606
Score = 233 bits (503), Expect = 1e-060
Identities = 89/135 (65%), Positives = 110/135 (81%)
Frame = -2 / +1
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLPEM+PKL K++FLDDD+VV++DL GLW I+M GKVNGAVETC
Sbjct: 202 RNPKYLSMLNHLRFYLPEMYPKLQKILFLDDDVVVQRDLTGLWKIDMDGKVNGAVETCFG 381
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
SFHRY +Y+NFS+P+I F+P+AC WA+GMN FDL WRR+ TE YH WQ NE+R L
Sbjct: 382 SFHRYWQYMNFSHPLIKAKFNPNACGWAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLL 561
Query: 428 WKLGTLPLCLVTFWN 384
WKLGTLP L+TF++
Sbjct: 562 WKLGTLPPGLITFYS 606
>gb|AV931472.1|AV931472 AV931472 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd19o15 3', mRNA sequence
Length = 635
Score = 231 bits (499), Expect = 4e-060
Identities = 88/99 (88%), Positives = 91/99 (91%)
Frame = -2 / -2
Query: 467 YHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIH 288
YH WQKLNED LWKLGTLP LVTFWNKTFPL RSWHVLGLGYNPHV+SRD+E AAVIH
Sbjct: 634 YHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIH 455
Query: 287 YNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP* 171
YNGN+KPWLEIGLPKFRSYWSKYLDYDQ FLRECNINP*
Sbjct: 454 YNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLRECNINP* 338
Score = 181 bits (390), Expect = 4e-045
Identities = 81/98 (82%), Positives = 84/98 (85%)
Frame = +1 / +3
Query: 169 LHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRP 348
LHGLMLHSRRK S*SRY DQ*LRNLGRPIS+QGF LPL*CMTAA S SLLLT GL*PRP
Sbjct: 336 LHGLMLHSRRKG*S*SRYFDQ*LRNLGRPISSQGFRLPL*CMTAARSRSLLLTCGL*PRP 515
Query: 349 RTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE 462
RTCHDR RGNVLFQNVT+ GRVPSF S SSLSFC +
Sbjct: 516 RTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSSLSFCQK 629
Score = 149 bits (319), Expect = 2e-035
Identities = 63/99 (63%), Positives = 79/99 (79%)
Frame = +3 / +2
Query: 171 SWIDVAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TK 350
S IDVAFP++RL+I+++L PVA +LG NL+PGL VAIVMYDC L VS M +VT*T+
Sbjct: 338 SRIDVAFPQKRLIIVKVL*PVASELGEANLKPGLQVAIVMYDCGTLQVSTAYMWVVT*TE 517
Query: 351 NMP*PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVI 467
NMP* EG+CLVPEC+QT RES KFP S+ +KLLP+V+
Sbjct: 518 NMP*SIEGECLVPECNQTWRESAKFP*QSVLVKLLPKVV 634
Score = 127 bits (271), Expect = 1e-028
Identities = 59/98 (60%), Positives = 65/98 (66%)
Frame = +2 / +1
Query: 170 FMD*CCIPEGKIGHNPDTWTSSSETWVDQSPTRASCCHCNV*LQHAQCLCC*HGDCNLDQ 349
F D*CCIP K H+ T TSS TW QS RAS CHCNV*L+HA L C H CNLD+
Sbjct: 337 FTD*CCIPAEKADHSQGTLTSSFGTWGGQSQARASGCHCNV*LRHAPGLYCLHVGCNLDR 516
Query: 350 EHAMTG*GEMSCSRM*PNTKGEYQVSRVIYLH*ASATS 463
EHAM G MSCSRM*P+ +GE QVS + *ASA S
Sbjct: 517 EHAMIDRGGMSCSRM*PDLEGECQVSIAVRPR*ASAKS 630
Score = 126 bits (270), Expect = 1e-028
Identities = 63/98 (64%), Positives = 66/98 (67%)
Frame = -3 / -3
Query: 463 TRGRSLMKIDHSGNLVLSLCVWSHSGTRHFPSAGHGMFLV*VTIPMLTAETLSMLQSYIT 284
T GRSL + D GNL LSL VW HSGTRH PS HGMF V*VT M ET S+ QSYIT
Sbjct: 630 TFGRSLTRTDCYGNLALSLQVWLHSGTRHSPSIDHGMFSV*VTTHM*AVETWSVPQSYIT 451
Query: 283 MAT*SPGWRLVYPSFGATGPSIWIMTNLSFGNATSIHE 170
MAT*SPG RL PS ATG S M +L GNATSI E
Sbjct: 450 MAT*SPGLRLASPSSEATGQSTLTMISLFCGNATSIRE 337
Score = 126 bits (270), Expect = 1e-028
Identities = 60/101 (59%), Positives = 71/101 (70%)
Frame = -1 / -1
Query: 468 LSLVAEA**R*ITLETWYSPFVFGHILEQDISPQPVMACSWSRLQSPC*QQRH*ACCSHT 289
L L+AEA* +ETW+SP G+ILEQDI P+ +MACS SRLQ C Q R AC SHT
Sbjct: 635 LPLLAEA*RGRTAMETWHSPSRSGYILEQDIPPRSIMACSRSRLQPTCKQ*RPGACRSHT 456
Query: 288 LQWQHEALVGDWSTQVSELLVQVSGL*PIFPSGMQHQSMKE 166
LQWQ EAL DW QV +LLV+V L* F +GMQHQS+K+
Sbjct: 455 LQWQPEALA*DWPPQVPKLLVKVP*L*SAFSAGMQHQSVKQ 333
>gb|BU968630.1|BU968630 HB08B19r BC Hordeum vulgare subsp. vulgare cDNA clone HB08B19
5-PRIME, mRNA sequence
Length = 521
Score = 197 bits (425), Expect(2) = 4e-059
Identities = 71/105 (67%), Positives = 93/105 (88%)
Frame = -2 / +2
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLP+++PKLDK++FLDDDIVV+KDL GLW +++ G VNGAV TCGE
Sbjct: 128 RNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNGAVFTCGE 307
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNIT 474
SFHR+D+YLNFSNP I ++FDP+AC WA+GMN+FDL +W+ ++IT
Sbjct: 308 SFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDIT 442
Score = 116 bits (248), Expect(2) = 3e-029
Identities = 52/83 (62%), Positives = 57/83 (68%)
Frame = +1 / -3
Query: 493 HSARSNTFIPKAQTQACGSKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPFMLIDQRPA 672
H RS FIP AQ QA GSK + GFEK RY S RWKLSPQV+TAP T PF P
Sbjct: 423 HCFRSKMFIP*AQPQALGSKFRAMCGFEKLRYLSKRWKLSPQVNTAPLTIPFRSTSHNPV 244
Query: 673 RSFFTTMSSSRKTILSSFGCISG 741
+SF TTMSSSRK+ILS+FG I G
Sbjct: 243 KSFCTTMSSSRKSILSNFG*ICG 175
Score = 51.0 bits (105), Expect(2) = 4e-059
Identities = 18/28 (64%), Positives = 21/28 (75%)
Frame = -2 / +3
Query: 491 RRQNITEIYHSWQKLNEDRSLWKLGTLP 408
R + + IYH WQ +NEDR LWKLGTLP
Sbjct: 426 RTKILPXIYHKWQNMNEDRVLWKLGTLP 509
Score = 31.8 bits (63), Expect(2) = 3e-029
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +1 / -2
Query: 409 GRVPSFQSDLSSLSFCH 459
G VPSFQ LSS FCH
Sbjct: 508 GSVPSFQRTLSSFMFCH 458
>gb|BU979822.1|BU979822 HA17H16r HA Hordeum vulgare subsp. vulgare cDNA clone HA17H16
5-PRIME, mRNA sequence
Length = 554
Score = 224 bits (484), Expect = 4e-058
Identities = 80/118 (67%), Positives = 106/118 (89%)
Frame = -2 / +3
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLP+++PKL+K++FLDDDIVV+KDL GLW +++ G VNGAVETCGE
Sbjct: 201 RNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQKDLTGLWEVDLNGNVNGAVETCGE 380
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDR 435
SFHR+D+YLNFSNP I+++FDP+AC WA+GMN+FDL EW++++IT IYH WQ +NE+R
Sbjct: 381 SFHRFDKYLNFSNPNISQNFDPNACGWAYGMNMFDLEEWKKKDITGIYHKWQNMNENR 554
Score = 128 bits (274), Expect = 4e-029
Identities = 60/98 (61%), Positives = 64/98 (65%)
Frame = +1 / -2
Query: 436 LSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSKLLVIMGFEKFRYRS*RWKLSP 615
L S FCH *I VM HS+RSN FIP AQ A GSK I GFEK RY S RWKLSP
Sbjct: 553 LFSFMFCHLW*IPVMSFFFHSSRSNIFIP*AQPHALGSKFCEIFGFEKLRYLSNRWKLSP 374
Query: 616 QVSTAPFTFPFMLIDQRPARSFFTTMSSSRKTILSSFG 729
VSTAPFTFP P +SF+TTMSSSRK IL + G
Sbjct: 373 HVSTAPFTFPLRSTSHSPVKSFWTTMSSSRKRILFNLG 260
>gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_D08 5', mRNA sequence
Length = 464
Score = 221 bits (476), Expect = 5e-057
Identities = 84/134 (62%), Positives = 108/134 (80%)
Frame = -2 / +3
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFY+PE++P+L K+VFLDDDIVV+KDL+ L++IN+ G V GAVETC E
Sbjct: 36 RNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETCME 215
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
+FHR+ +YLN S+P+I FDP AC WAFGMNV DL EWR +N+T IYH WQ+ N D +L
Sbjct: 216 TFHRFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDLVEWRNKNVTGIYHYWQERNADHTL 395
Query: 428 WKLGTLPLCLVTFW 387
WKLG+LP L+ F+
Sbjct: 396 WKLGSLPPGLLAFY 437
Score = 109 bits (233), Expect = 2e-023
Identities = 61/111 (54%), Positives = 66/111 (59%)
Frame = +1 / -2
Query: 409 GRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSKLLVIMGFEKFRY 588
G+ PSF S+L C * I V F LHS RS+TFIP AQ QA GSK I G E RY
Sbjct: 415 GKDPSFHRV*SALRSCQ**YIPVTFLFLHSTRSSTFIPNAQPQASGSK*ARIRG*E*LRY 236
Query: 589 RS*RWKLSPQVSTAPFTFPFMLIDQRPARSFFTTMSSSRKTILSSFGCISG 741
W +S QVSTAP T P LI + RSF TTMSSSR T L S G ISG
Sbjct: 235 L*NLWNVSMQVSTAPITLPLRLIVNKSDRSF*TTMSSSRNTTLCSSG*ISG 83
>gb|BJ549963.1|BJ549963 BJ549963 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 3', mRNA sequence
Length = 625
Score = 220 bits (475), Expect = 7e-057
Identities = 78/126 (61%), Positives = 100/126 (79%)
Frame = -2 / -3
Query: 551 FDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFP 372
F+P+AC WA+GMN FDL WRR+ TE YH WQ NE+R LWKLGTLP L+TF++ T P
Sbjct: 614 FNPNACGWAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLWKLGTLPPGLITFYSTTKP 435
Query: 371 LSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLR 192
L +SWHVLGLGYNP ++ +I +AAV+H+NGNMKPWL+IG+ +FR W+KY+DYD SF+R
Sbjct: 434 LDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIR 255
Query: 191 ECNINP 174
+CN P
Sbjct: 254 QCNFAP 237
>gb|BM444949.1|BM444949 EBem09_SQ006_P20_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ006_P20 5', mRNA sequence
Length = 417
Score = 217 bits (469), Expect = 5e-056
Identities = 82/127 (64%), Positives = 104/127 (81%)
Frame = -2 / +3
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFY+PE++P+L K+VFLDDDIVV+KDL+ L++IN+ G V GAVETC E
Sbjct: 36 RNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETCME 215
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
+FHR+ +YLN S+P+I FDP AC WAFGMNV DL EWR +N+T IYH WQ+ N D +L
Sbjct: 216 TFHRFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDLVEWRNKNVTGIYHYWQERNADHTL 395
Query: 428 WKLGTLP 408
WKLG+LP
Sbjct: 396 WKLGSLP 416
Score = 109 bits (233), Expect = 2e-023
Identities = 61/111 (54%), Positives = 66/111 (59%)
Frame = +1 / -3
Query: 409 GRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSKLLVIMGFEKFRY 588
G+ PSF S+L C * I V F LHS RS+TFIP AQ QA GSK I G E RY
Sbjct: 415 GKDPSFHRV*SALRSCQ**YIPVTFLFLHSTRSSTFIPNAQPQASGSK*ARIRG*E*LRY 236
Query: 589 RS*RWKLSPQVSTAPFTFPFMLIDQRPARSFFTTMSSSRKTILSSFGCISG 741
W +S QVSTAP T P LI + RSF TTMSSSR T L S G ISG
Sbjct: 235 L*NLWNVSMQVSTAPITLPLRLIVNKSDRSF*TTMSSSRNTTLCSSG*ISG 83
>gb|AV930935.1|AV930935 AV930935 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25o11 3', mRNA sequence
Length = 495
Score = 214 bits (461), Expect = 6e-055
Identities = 82/92 (89%), Positives = 85/92 (92%)
Frame = -2 / -1
Query: 446 NEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKP 267
NED LWKLGTLP LVTFWNKTFPL RSWHVLGLGYNPHV+SRD+E AAVIHYNGN+KP
Sbjct: 495 NEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYNGNLKP 316
Query: 266 WLEIGLPKFRSYWSKYLDYDQSFLRECNINP* 171
WLEIGLPKFRSYWSKYLDYDQ FLRECNINP*
Sbjct: 315 WLEIGLPKFRSYWSKYLDYDQPFLRECNINP* 220
Score = 170 bits (366), Expect = 8e-042
Identities = 77/92 (83%), Positives = 79/92 (85%)
Frame = +1 / +2
Query: 169 LHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRP 348
LHGLMLHSRRK S*SRY DQ*LRNLGRPIS+QGF LPL*CMTAA S SLLLT GL*PRP
Sbjct: 218 LHGLMLHSRRKG*S*SRYFDQ*LRNLGRPISSQGFRLPL*CMTAARSRSLLLTCGL*PRP 397
Query: 349 RTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSS 444
RTCHDR RGNVLFQNVT+ GRVPSF S SS
Sbjct: 398 RTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSS 493
Score = 135 bits (290), Expect = 2e-031
Identities = 58/92 (63%), Positives = 72/92 (78%)
Frame = +3 / +1
Query: 171 SWIDVAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TK 350
S IDVAFP++RL+I+++L PVA +LG NL+PGL VAIVMYDC L VS M +VT*T+
Sbjct: 220 SRIDVAFPQKRLIIVKVL*PVASELGEANLKPGLQVAIVMYDCGTLQVSTAYMWVVT*TE 399
Query: 351 NMP*PAEGKCLVPECDQTQRESTKFPE*SIFI 446
NMP* EG+CLVPEC+QT RES KFP S+ +
Sbjct: 400 NMP*SIEGECLVPECNQTWRESAKFP*QSVLV 495
Score = 122 bits (260), Expect = 3e-027
Identities = 54/86 (62%), Positives = 59/86 (68%)
Frame = +2 / +3
Query: 170 FMD*CCIPEGKIGHNPDTWTSSSETWVDQSPTRASCCHCNV*LQHAQCLCC*HGDCNLDQ 349
F D*CCIP K H+ T TSS TW QS RAS CHCNV*L+HA L C H CNLD+
Sbjct: 219 FTD*CCIPAEKADHSQGTLTSSFGTWGGQSQARASGCHCNV*LRHAPGLYCLHVGCNLDR 398
Query: 350 EHAMTG*GEMSCSRM*PNTKGEYQVS 427
EHAM G MSCSRM*P+ +GE QVS
Sbjct: 399 EHAMIDRGGMSCSRM*PDLEGECQVS 476
Score = 121 bits (259), Expect = 5e-027
Identities = 54/88 (61%), Positives = 64/88 (72%)
Frame = -1 / -3
Query: 429 LETWYSPFVFGHILEQDISPQPVMACSWSRLQSPC*QQRH*ACCSHTLQWQHEALVGDWS 250
+ETW+SP G+ILEQDI P+ +MACS SRLQ C Q R AC SHTLQWQ EAL DW
Sbjct: 478 METWHSPSRSGYILEQDIPPRSIMACSRSRLQPTCKQ*RPGACRSHTLQWQPEALA*DWP 299
Query: 249 TQVSELLVQVSGL*PIFPSGMQHQSMKE 166
QV +LLV+V L* F +GMQHQS+K+
Sbjct: 298 PQVPKLLVKVP*L*SAFSAGMQHQSVKQ 215
Score = 117 bits (250), Expect = 8e-026
Identities = 58/91 (63%), Positives = 61/91 (67%)
Frame = -3 / -2
Query: 442 KIDHSGNLVLSLCVWSHSGTRHFPSAGHGMFLV*VTIPMLTAETLSMLQSYITMAT*SPG 263
+ D GNL LSL VW HSGTRH PS HGMF V*VT M ET S+ QSYITMAT*SPG
Sbjct: 491 RTDCYGNLALSLQVWLHSGTRHSPSIDHGMFSV*VTTHM*AVETWSVPQSYITMAT*SPG 312
Query: 262 WRLVYPSFGATGPSIWIMTNLSFGNATSIHE 170
RL PS ATG S M +L GNATSI E
Sbjct: 311 LRLASPSSEATGQSTLTMISLFCGNATSIRE 219
>gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0018F09f, mRNA sequence
Length = 856
Score = 212 bits (457), Expect = 2e-054
Identities = 72/121 (59%), Positives = 97/121 (80%)
Frame = -2 / +1
Query: 539 ACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRS 360
AC WA+GMN+FDL +W+ ++IT IYH WQ +NEDR LWKLGTLP L+TF+ T PL +S
Sbjct: 1 ACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKS 180
Query: 359 WHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNI 180
WHVLGLGYNP ++ +I++AAV HYNGNMKPWLE+ + K+R YW++Y+ YD ++R CN+
Sbjct: 181 WHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNL 360
Query: 179 N 177
+
Sbjct: 361 S 363
Score = 105 bits (225), Expect = 2e-022
Identities = 61/123 (49%), Positives = 67/123 (54%)
Frame = +1 / -3
Query: 169 LHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRP 348
++ L LH R WS YL Q R L S+ GFM PL* TAA S+S GL P P
Sbjct: 371 IYSLRLHPRM*GWSYFIYLVQYGRYLVIASSSHGFMFPL**ATAALSISERSMLGLYPSP 192
Query: 349 RTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKA 528
RTCHD RG V NV + G VPSFQ LSS FCH *I V+ H RS FIP A
Sbjct: 191 RTCHDLSRGCVSL*NVMRPGGSVPSFQRTLSSFMFCHLW*IPVISLFFHCFRSKMFIP*A 12
Query: 529 QTQ 537
Q Q
Sbjct: 11 QPQ 3
>gb|BG417087.1|BG417087 HVSMEk0016D06f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0016D06f, mRNA sequence
Length = 490
Score = 190 bits (410), Expect = 7e-048
Identities = 65/128 (50%), Positives = 92/128 (71%)
Frame = -2 / +3
Query: 569 PIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTF 390
PII+ DP+ C WAFGMN+FDL WR+ N T +YH WQ+ N D+ LW+ GTLP L+TF
Sbjct: 6 PIISSKIDPYTCGWAFGMNIFDLIAWRKANATSLYHYWQEQNADQLLWRTGTLPAGLLTF 185
Query: 389 WNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDY 210
+ PL R WH+LGLGY+ ++ R IE AAV+HYNGNMKPWL++ + +++S W +++++
Sbjct: 186 YGLMEPLDRRWHLLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIHRYKSIWERHVNF 365
Query: 209 DQSFLREC 186
+REC
Sbjct: 366 SHPHIREC 389
>gb|AV837092.1|AV837092 AV837092 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd23f10, mRNA
sequence
Length = 643
Score = 183 bits (394), Expect = 1e-045
Identities = 62/107 (57%), Positives = 85/107 (79%)
Frame = -2 / -2
Query: 497 EWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNS 318
+W+ ++IT IYH WQ +NEDR LWKLGTLP L+TF+ T PL +SWHVLGLGYNP ++
Sbjct: 639 QWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDR 460
Query: 317 RDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNIN 177
+I++AAV HYNGNMKPWLE+ + K+R YW++Y+ YD ++R CN++
Sbjct: 459 SEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNLS 319
>gb|BJ468235.1|BJ468235 BJ468235 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 3', mRNA sequence
Length = 634
Score = 178 bits (383), Expect = 4e-044
Identities = 62/105 (59%), Positives = 83/105 (79%)
Frame = -2 / -1
Query: 491 RRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRD 312
R ++IT IYH WQ +NEDR LWKLGTLP L+TF+ T PL +SWHVLGLGYNP ++ +
Sbjct: 631 RTKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSE 452
Query: 311 IEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNIN 177
I++AAV HYNGNMKPWLE+ + K+R YW++Y+ YD ++R CN++
Sbjct: 451 IDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNLS 317
>gb|BI777829.2|BI777829 EBro08_SQ002_D17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ002_D17 5', mRNA sequence
Length = 562
Score = 131 bits (281), Expect(2) = 4e-043
Identities = 50/80 (62%), Positives = 66/80 (82%)
Frame = -2 / +2
Query: 779 RYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGESFH 600
+++S+LNHLRFY+P++ P L+K+VFLDDD+VV+KDL L+SI + G V GAVETC ESFH
Sbjct: 209 KFVSLLNHLRFYIPQILPNLEKVVFLDDDVVVQKDLTQLFSIELHGNVIGAVETCLESFH 388
Query: 599 RYDRYLNFSNPIITKSFDPH 540
RY +YLNFS PII+ DP+
Sbjct: 389 RYHKYLNFSQPIISSKIDPY 448
Score = 63.4 bits (132), Expect(2) = 4e-043
Identities = 21/36 (58%), Positives = 26/36 (72%)
Frame = -2 / +3
Query: 536 CVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSL 429
C WAFGMN+FDL WR+ N T +YH WQ+ N D+ L
Sbjct: 453 CGWAFGMNIFDLIAWRKANATSLYHYWQEQNADQLL 560
>gb|AV833832.1|AV833832 AV833832 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags20k03, mRNA sequence
Length = 526
Score = 158 bits (340), Expect(2) = 2e-042
Identities = 59/84 (70%), Positives = 75/84 (89%)
Frame = -2 / +2
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLP+++PKLDK++FLDDDIVV+KDL GLW +++ G VNGAV TCGE
Sbjct: 224 RNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNGAVFTCGE 403
Query: 608 SFHRYDRYLNFSNPIITKSFDPHA 537
SFHR+D+YLNFSNP I ++FDP+A
Sbjct: 404 SFHRFDKYLNFSNPHIARNFDPNA 475
Score = 101 bits (216), Expect = 4e-021
Identities = 45/73 (61%), Positives = 50/73 (68%)
Frame = +1 / -2
Query: 523 KAQTQACGSKLLVIMGFEKFRYRS*RWKLSPQVSTAPFTFPFMLIDQRPARSFFTTMSSS 702
K QA GSK + GFEK RY S RWKLSPQV+TAP T PF P +SF TTMSSS
Sbjct: 489 KPNHQALGSKFRAMCGFEKLRYLSKRWKLSPQVNTAPLTIPFRSTSHNPVKSFCTTMSSS 310
Query: 703 RKTILSSFGCISG 741
RK+ILS+FG I G
Sbjct: 309 RKSILSNFG*ICG 271
Score = 34.1 bits (68), Expect(2) = 2e-042
Identities = 9/14 (64%), Positives = 13/14 (92%)
Frame = -2 / +3
Query: 530 WAFGMNVFDLAEWR 489
WA+GMN+FDL +W+
Sbjct: 483 WAYGMNIFDLKQWK 524
>gb|CA018769.1|CA018769 HV09J15r HV Hordeum vulgare subsp. vulgare cDNA clone HV09J15
5-PRIME, mRNA sequence
Length = 586
Score = 171 bits (369), Expect = 3e-042
Identities = 64/102 (62%), Positives = 82/102 (80%)
Frame = -2 / +2
Query: 779 RYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGESFH 600
+++S+LNHLRFY+P++ P L+K+VFLDDD+VV+KDL L+SI + G V GAVETC ESFH
Sbjct: 278 KFVSLLNHLRFYIPQILPNLEKVVFLDDDVVVQKDLTQLFSIELHGNVIGAVETCLESFH 457
Query: 599 RYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNIT 474
RY +YLNFS PII+ DP+ C WAFGMN+FDL WR+ N T
Sbjct: 458 RYHKYLNFSQPIISSKIDPYTCGWAFGMNIFDLIAWRKANAT 583
>gb|BF266558.3|BF266558 HV_CEa0015H07f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0015H07f, mRNA sequence
Length = 767
Score = 149 bits (321), Expect(2) = 1e-041
Identities = 58/94 (61%), Positives = 73/94 (77%)
Frame = -2 / +3
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFYLPEM+PKL K++FLDDD+VV++DL GLW I+M GKVNGAVETC
Sbjct: 408 RNPKYLSMLNHLRFYLPEMYPKLQKILFLDDDVVVQRDLTGLWKIDMDGKVNGAVETCFG 587
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVF 507
SFHR +Y+NFS+P+I +P+AC W F
Sbjct: 588 SFHRDWQYMNFSHPLIKAKVNPNACGWGLWHETF 689
Score = 40.0 bits (81), Expect(2) = 1e-041
Identities = 16/31 (51%), Positives = 19/31 (61%)
Frame = -2 / +1
Query: 527 AFGMNVFDLAEWRRQNITEIYHSWQKLNEDR 435
A+GM +FDL RR T YH WQ NE+R
Sbjct: 670 AYGMKLFDLKS*RRGESTRQYHYWQTQNENR 762
>gb|AV909171.1|AV909171 AV909171 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11o03 5', mRNA sequence
Length = 586
Score = 168 bits (361), Expect = 4e-041
Identities = 59/95 (62%), Positives = 77/95 (81%)
Frame = -2 / +2
Query: 458 WQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNG 279
WQ NE+R LWKLGTLP L+TF++ T PL +SWHVLGLGYNP ++ +I +AAV+H+NG
Sbjct: 8 WQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNG 187
Query: 278 NMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP 174
NMKPWL+IG+ +FR W+KY+DYD SF+R+CN P
Sbjct: 188 NMKPWLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 292
>gb|AV911054.1|AV911054 AV911054 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11o03 3', mRNA sequence
Length = 527
Score = 168 bits (361), Expect = 4e-041
Identities = 59/95 (62%), Positives = 77/95 (81%)
Frame = -2 / -2
Query: 458 WQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNG 279
WQ NE+R LWKLGTLP L+TF++ T PL +SWHVLGLGYNP ++ +I +AAV+H+NG
Sbjct: 520 WQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNG 341
Query: 278 NMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP 174
NMKPWL+IG+ +FR W+KY+DYD SF+R+CN P
Sbjct: 340 NMKPWLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 236
>gb|BJ544949.1|BJ544949 BJ544949 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41d13 5', mRNA sequence
Length = 589
Score = 168 bits (361), Expect = 4e-041
Identities = 59/95 (62%), Positives = 77/95 (81%)
Frame = -2 / +2
Query: 458 WQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNG 279
WQ NE+R LWKLGTLP L+TF++ T PL +SWHVLGLGYNP ++ +I +AAV+H+NG
Sbjct: 11 WQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNG 190
Query: 278 NMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP 174
NMKPWL+IG+ +FR W+KY+DYD SF+R+CN P
Sbjct: 191 NMKPWLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 295
>gb|BJ546433.1|BJ546433 BJ546433 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41d13 3', mRNA sequence
Length = 525
Score = 168 bits (361), Expect = 4e-041
Identities = 59/95 (62%), Positives = 77/95 (81%)
Frame = -2 / -1
Query: 458 WQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNG 279
WQ NE+R LWKLGTLP L+TF++ T PL +SWHVLGLGYNP ++ +I +AAV+H+NG
Sbjct: 519 WQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNG 340
Query: 278 NMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP 174
NMKPWL+IG+ +FR W+KY+DYD SF+R+CN P
Sbjct: 339 NMKPWLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 235
>gb|BM372595.2|BM372595 EBpi05_SQ003_G13_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_G13 5', mRNA sequence
Length = 523
Score = 163 bits (351), Expect = 9e-040
Identities = 56/94 (59%), Positives = 75/94 (79%)
Frame = -2 / +2
Query: 458 WQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNG 279
WQ +NEDR LWKLGTLP L+TF+ T PL +SWHVLGLGYNP ++ +I++AAV HYNG
Sbjct: 2 WQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNG 181
Query: 278 NMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNIN 177
NMKPWLE+ + K+R YW++Y+ YD ++R CN++
Sbjct: 182 NMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNLS 283
>gb|BJ463761.1|BJ463761 BJ463761 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g13 5', mRNA sequence
Length = 596
Score = 162 bits (349), Expect = 2e-039
Identities = 63/96 (65%), Positives = 80/96 (83%)
Frame = -2 / +3
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFY+PE++P+L K+VFLDDDIVV+KDL+ L++IN+ G V GAVETC E
Sbjct: 306 RNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETCME 485
Query: 608 SFHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDL 501
+FHR+ +YLN S+P+I FDP AC WAFGMNV DL
Sbjct: 486 TFHRFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDL 593
>gb|AV833846.1|AV833846 AV833846 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags20p21, mRNA sequence
Length = 494
Score = 84.5 bits (178), Expect(3) = 5e-038
Identities = 29/52 (55%), Positives = 36/52 (69%)
Frame = -2 / +3
Query: 599 RYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLN 444
R+ Y NFS+P+I +S DP C WA+GMNVFDL WR+ NI + YH W K N
Sbjct: 33 RFRTYFNFSHPVIDRSLDPDECAWAYGMNVFDLEAWRKTNIRDTYHFWLKEN 188
Score = 82.2 bits (173), Expect(3) = 5e-038
Identities = 30/67 (44%), Positives = 40/67 (59%)
Frame = -2 / +3
Query: 455 QKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGN 276
+ L +LWK GTLP L+ F + SWH+LGLGY + ++ AAV+HYNG
Sbjct: 183 ENLKAGLTLWKFGTLPPALIAFRGHVHGIDPSWHMLGLGYQESTDIESVKKAAVVHYNGQ 362
Query: 275 MKPWLEI 255
KPWL+I
Sbjct: 363 CKPWLDI 383
Score = 29.5 bits (58), Expect(3) = 5e-038
Identities = 7/18 (38%), Positives = 14/18 (77%)
Frame = -2 / +1
Query: 233 YWSKYLDYDQSFLRECNI 180
+W+K+++Y F+R C+I
Sbjct: 406 FWTKHVNYSNDFIRNCHI 459
>gb|CK124684.1|CK124684 BES1824109p11 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P119 5-PRIME, mRNA sequence
Length = 787
Score = 127 bits (272), Expect(2) = 4e-037
Identities = 49/80 (61%), Positives = 67/80 (83%)
Frame = -2 / +1
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFY+PE++P+L K++FLDDDIVV+KDL+ L++IN+ G V GAVETC E
Sbjct: 451 RNPKYLSMLNHLRFYIPEIYPELHKVLFLDDDIVVQKDLSDLFTINLNGNVMGAVETCME 630
Query: 608 SFHRYDRYLNFSNPIITKSF 549
+FHR+ +YLN S+P+I F
Sbjct: 631 TFHRFHKYLNHSHPLIRAHF 690
Score = 47.3 bits (97), Expect(2) = 4e-037
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = -2 / +2
Query: 551 FDPHACVWAFGMNVFDLAEWRRQNI 477
FDP AC WAFGMNV DL EW + +
Sbjct: 689 FDPDACGWAFGMNVLDLXEWXTEMV 763
>gb|BJ467937.1|BJ467937 BJ467937 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g13 3', mRNA sequence
Length = 703
Score = 99.6 bits (211), Expect(2) = 1e-035
Identities = 37/63 (58%), Positives = 45/63 (71%)
Frame = -2 / -1
Query: 524 FGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLG 345
FGMNV DL EWR +N+T IYH WQ+ N D +LWKLG+LP L+ F+ L+ WHVLG
Sbjct: 703 FGMNVLDLVEWRNKNVTGIYHYWQERNADHTLWKLGSLPPGLLAFYGLVEALNPKWHVLG 524
Query: 344 LGY 336
LGY
Sbjct: 523 LGY 515
Score = 70.7 bits (148), Expect(2) = 1e-035
Identities = 22/47 (46%), Positives = 33/47 (70%)
Frame = -2 / -1
Query: 326 VNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLREC 186
V+ I+ AV+HYNGN+KPWL+IG+ K++ +W Y+DY L+ C
Sbjct: 508 VDPDTIKEGAVLHYNGNLKPWLKIGMEKYKGFWDNYVDYSHPLLQRC 368
>gb|CB881764.1|CB881764 HM10N01w HM Hordeum vulgare subsp. vulgare cDNA clone HM10N01
3-PRIME, mRNA sequence
Length = 535
Score = 146 bits (313), Expect = 2e-034
Identities = 51/83 (61%), Positives = 68/83 (81%)
Frame = -2 / -3
Query: 422 LGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPK 243
LGTLP L+TF++ T PL +SWHVLGLGYNP ++ +I +AAV+H+NGNMKPWL+IG+ +
Sbjct: 533 LGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQ 354
Query: 242 FRSYWSKYLDYDQSFLRECNINP 174
FR W+KY+DYD SF+R+CN P
Sbjct: 353 FRQLWTKYVDYDDSFIRQCNFAP 285
>gb|AJ432742.1|AJ432742 AJ432742 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200078D03F1, mRNA sequence
Length = 447
Score = 127 bits (273), Expect = 5e-029
Identities = 42/75 (56%), Positives = 60/75 (80%)
Frame = -2 / +1
Query: 401 LVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSK 222
L+TF+ T PL +SWHVLGLGYNP ++ +I++AAV HYNGNMKPWLE+ + K+R YW++
Sbjct: 7 LMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTR 186
Query: 221 YLDYDQSFLRECNIN 177
Y+ YD ++R CN++
Sbjct: 187 YIKYDHPYIRGCNLS 231
>gb|AV834161.1|AV834161 AV834161 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags5h22, mRNA sequence
Length = 714
Score = 96.8 bits (205), Expect(2) = 7e-027
Identities = 34/79 (43%), Positives = 53/79 (67%)
Frame = -2 / -1
Query: 407 LCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYW 228
L L++F N +PL + GLGY+ ++ + +A +HYNGNMKPWLE+G+P ++ YW
Sbjct: 555 LSLLSFQNLIYPLDGRSTLSGLGYDYGIDPEVAQSSAALHYNGNMKPWLELGIPDYKKYW 376
Query: 227 SKYLDYDQSFLRECNINP* 171
++L + F+ ECN+NP*
Sbjct: 375 RRFLTREDRFMDECNVNP* 319
Score = 43.7 bits (89), Expect(2) = 7e-027
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -2 / -1
Query: 551 FDPHACVWAFGMNVFDLAEWRRQNITEIY 465
+DP +C W G+NV +L +WR+ +TE Y
Sbjct: 705 YDPKSCAWMSGVNVINLDKWRKHKVTENY 619
>gb|BU986456.1|BU986456 HF11O19r HF Hordeum vulgare subsp. vulgare cDNA clone HF11O19
5-PRIME, mRNA sequence
Length = 478
Score = 117 bits (250), Expect = 8e-026
Identities = 43/90 (47%), Positives = 61/90 (67%)
Frame = -2 / +3
Query: 434 SLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEI 255
+L LP L+TF+ T PL +SWHV+GLGYNP ++ I AAVIH+NGNMKPWL++
Sbjct: 24 ALGSTSVLPAGLMTFYGNTKPLDKSWHVMGLGYNPSISPEVIRGAAVIHFNGNMKPWLDV 203
Query: 254 GLPKFRSYWSKYLDYDQSFLRECNINP*RN 165
++++ W+K++D + FL CN *RN
Sbjct: 204 AFNQYKALWTKHVDTEMEFLTLCNFGL*RN 293
>gb|AV834211.1|AV834211 AV834211 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags7a24, mRNA sequence
Length = 663
Score = 112 bits (240), Expect = 2e-024
Identities = 41/97 (42%), Positives = 62/97 (63%)
Frame = -2 / -1
Query: 446 NEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKP 267
N+D + + LP+ L++F N +PL + GLGY+ + I +A +HYNGNMKP
Sbjct: 648 NDDEASLRAAALPISLLSFQNLVYPLDERLTLSGLGYHYGIEEEVIRTSASLHYNGNMKP 469
Query: 266 WLEIGLPKFRSYWSKYLDYDQSFLRECNINP*RNLVL 156
WLE+G+P +R YW ++L D+ F+ ECN++P* L L
Sbjct: 468 WLELGIPNYRKYWKRFLARDERFMDECNVSP*HTLDL 358
>gb|BJ463762.1|BJ463762 BJ463762 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g14 5', mRNA sequence
Length = 500
Score = 111 bits (237), Expect = 5e-024
Identities = 43/65 (66%), Positives = 57/65 (87%)
Frame = -2 / +2
Query: 788 RPGRYLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGE 609
R +YLS+LNHLRFY+PE++P+L K+VFLDDDIVV+KDL+ L++IN+ G V GAVETC E
Sbjct: 305 RNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETCME 484
Query: 608 SFHRY 594
+FHR+
Sbjct: 485 TFHRF 499
>gb|BQ460226.1|BQ460226 HA06N23r HA Hordeum vulgare subsp. vulgare cDNA clone HA06N23
5-PRIME, mRNA sequence
Length = 567
Score = 107 bits (228), Expect = 9e-023
Identities = 36/85 (42%), Positives = 53/85 (62%)
Frame = -2 / +2
Query: 434 SLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEI 255
+LWK GTLP L+ F + SWH+LGLGY + ++ AAV+HYNG KPWL+I
Sbjct: 20 TLWKFGTLPPALIAFRGHVHGIDPSWHMLGLGYQESTDIESVKKAAVVHYNGQCKPWLDI 199
Query: 254 GLPKFRSYWSKYLDYDQSFLRECNI 180
+ +W+K+++Y F+R C+I
Sbjct: 200 AFKNLQPFWTKHVNYSNDFIRNCHI 274
>gb|AL510176.1|AL510176 AL510176 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04C08u 3',
mRNA sequence
Length = 548
Score = 73.9 bits (155), Expect(2) = 3e-021
Identities = 22/40 (55%), Positives = 32/40 (80%)
Frame = -2 / -3
Query: 296 VIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLRECNIN 177
V HYNGNMKPWLE+ + K+R YW++Y+ YD ++R CN++
Sbjct: 432 VAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNLS 313
Score = 47.8 bits (98), Expect(2) = 3e-021
Identities = 16/26 (61%), Positives = 22/26 (84%)
Frame = -2 / -2
Query: 374 PLSRSWHVLGLGYNPHVNSRDIEHAA 297
PL +SWHVLGLGYNP ++ +I++AA
Sbjct: 511 PLDKSWHVLGLGYNPSIDRSEIDNAA 434
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 407,785,783
Number of Sequences: 312970
Number of extensions: 6905105
Number of successful extensions: 43958
Number of sequences better than 1.0e-020: 59
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 8968096407
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)