TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 5704580.2.1
         (721 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BU974788.1|BU974788  HB29B20r BC Hordeum vulgare subsp. v...   123   1e-027

>gb|BU974788.1|BU974788 HB29B20r BC Hordeum vulgare subsp. vulgare cDNA clone HB29B20
           5-PRIME, mRNA sequence
          Length = 457

 Score =  123 bits (263), Expect = 1e-027
 Identities = 53/86 (61%), Positives = 61/86 (70%)
 Frame = -3 / -2

                                                                       
Query: 392 GIPKKEAKEVVQECKEIYENSLVDGNPQKSGFVFGKLKCLTAKKGIWRKLGQ*VWT*AEW 213
           GIP+KEAKEVVQECKEIYENSL+DGNP KSGFVFGKLKCLT KKGIW KLGQ* +   E 
Sbjct: 456 GIPRKEAKEVVQECKEIYENSLIDGNPPKSGFVFGKLKCLTVKKGIWGKLGQ*EYASTEH 277

                                     
Query: 212 TNVPPRQRLYVSLLYDHSFVLLSMLL 135
                +        +    +LLS+L+
Sbjct: 276 CKCTHKDGFVFYFAHSFVHLLLSLLV 199




 Score =  115 bits (246), Expect = 2e-025
 Identities = 45/59 (76%), Positives = 49/59 (83%)
 Frame = +1 / +2

                                                                      
Query: 217 SAQVHTYCPSFLHMPFFAVKHFNLPNTKPDF*GLPSTSEFSYISLHSCTTSLASFFGMP 393
           S   +++CPSF HMPFF V+HFNLPNT PDF GLPS  EFSYISLHSCTTSLASF GMP
Sbjct: 281 SVLAYSHCPSFPHMPFFTVRHFNLPNTNPDFGGLPSMREFSYISLHSCTTSLASFRGMP 457



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 315,838,279
Number of Sequences: 312970
Number of extensions: 4528808
Number of successful extensions: 39932
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7974341292
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)