TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4790360.2.1
(667 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa... 214 5e-055
gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgar... 190 7e-048
gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa... 103 4e-041
>gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0003F21f, mRNA sequence
Length = 625
Score = 214 bits (461), Expect = 5e-055
Identities = 86/117 (73%), Positives = 90/117 (76%)
Frame = +2 / +3
Query: 41 LHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNHENTHALCEPDCTE 220
LHAYGPIY RNNSFRMF+NEDVTIGSWMLAMNVNHENTHALCEP+CT
Sbjct: 3 LHAYGPIYALSADVVASLVALRNNSFRMFNNEDVTIGSWMLAMNVNHENTHALCEPECTA 182
Query: 221 SSIAVWDIPKCSGLCHPEVKMLELHERKECTGGPTVAAEVSESED**FLGWGTNYGS 391
SSIAVWDIPKCSGLCHPEVKMLELH RKECTGGPT AAE ++* LG T S
Sbjct: 183 SSIAVWDIPKCSGLCHPEVKMLELHRRKECTGGPTEAAETETDDE*CLLGLETQGAS 353
Score = 186 bits (400), Expect = 1e-046
Identities = 83/113 (73%), Positives = 87/113 (76%)
Frame = -2 / -1
Query: 378 VPQPRNHQSSDSDTSAATVGPPVHXXXXXXXXXXXSGWHRPEHFGMSQTAIEDSVQSGSQ 199
V +P H SS SAA+VGPPVH SGWH PEHFGMSQTAIED+V SGSQ
Sbjct: 340 VSRPSKHHSSSVSVSAASVGPPVHSFRR*SSSIFTSGWHSPEHFGMSQTAIEDAVHSGSQ 161
Query: 198 SACVFSWLTFIASIHDPIVTSSLLNIRKLLFRNATKLATTSADKA*IGPYA*R 40
A VFSWLTFIASI DP+VTSSLLNIRKLLF ATKLATTSADKA*IGPYA*R
Sbjct: 160 RAWVFSWLTFIASIQDPMVTSSLLNIRKLLFLRATKLATTSADKA*IGPYA*R 2
Score = 177 bits (381), Expect = 5e-044
Identities = 71/113 (62%), Positives = 85/113 (75%)
Frame = +1 / +2
Query: 40 PSCIWANLCFIS*RGG*LGCIAKQQFPYVQQRGRYNWIVDACYERQP*EHTCTL*T*LHG 219
PSCIWA LCFIS* GG LGC KQQFP VQQRGRY+WI+DACYERQP EH C L +HG
Sbjct: 2 PSCIWAYLCFIS*CGGKLGCSEKQQFPNVQQRGRYHWILDACYERQPREHPCPLRARVHG 181
Query: 220 VFNCCLGHPKMLRSMPPRGKNARAP*KKRMHRWSNCGS*GVRV*GLMISWLGN 378
+ +C LGHPKM R+MPPRGK+A A +R+HRWS+ G * +M++W G+
Sbjct: 182 ILDCRLGHPKMFRAMPPRGKDAGASPAERVHRWSDGGGRNRNR*RVMLAWSGD 340
Score = 152 bits (326), Expect = 2e-036
Identities = 67/112 (59%), Positives = 78/112 (69%)
Frame = -3 / -2
Query: 374 PNQEIINPQTRTPQLPQLDHLCILFFHGALAFLPLGGIDLSILGCPKQQLKTPCSQVHKV 195
P+Q I + P DH C L A A LPLGGI L+ILGCP++Q + PC++ +
Sbjct: 336 PDQASITRHRFRFRPPPSDHRCTLSAGEAPASLPLGGIALNILGCPRRQSRMPCTRARRG 157
Query: 194 HVCSHG*RS*QASTIQL*RPRC*TYGNCCFAMQPS*PPRQLIKHKLAHMHEG 39
H CS G*RS*QAS IQ *RPRC*T+GNCCF+ QPS PP QLIKHK AHMHEG
Sbjct: 156 HGCSRG*RS*QASRIQW*RPRC*TFGNCCFSEQPSLPPHQLIKHK*AHMHEG 1
Score = 137 bits (293), Expect = 7e-032
Identities = 62/93 (66%), Positives = 70/93 (75%)
Frame = -1 / -3
Query: 319 TTCAFFSFMEL*HFYLWVA*T*AFWDVPNSN*RLRAVRFTKCMCVLMVDVHSKHPRSNCN 140
TT A F ++L H YLWVA* * FWDVP+ N RA+ + M VL+VDVHSKHP SN N
Sbjct: 281 TTGALFPPVKLQHLYLWVA*P*TFWDVPDGNRGCRALGLAEGMGVLVVDVHSKHPGSNGN 102
Query: 139 VLVAEHTETVVSQCNQASHHVS**SINWPICMK 41
VLV EH+ETVVSQ NQA HH+S**SIN PICMK
Sbjct: 101 VLVVEHSETVVSQSNQACHHIS**SINRPICMK 3
Score = 131 bits (281), Expect = 3e-030
Identities = 62/95 (65%), Positives = 68/95 (71%)
Frame = +3 / +1
Query: 39 SFMHMGQFMLYQLTWWLAWLHCETTVSVCSATRTLQLDRGCLL*TSTMRTHMHFVNLTAR 218
SFMHMG FMLYQL WW AWL ETTVS CS TRTL LD GCLL*TST RT M + +AR
Sbjct: 1 SFMHMGLFMLYQLMWWQAWLL*ETTVSECSTTRTLPLDPGCLL*TSTTRTPMPSASPSAR 180
Query: 219 SLQLLFGTSQNAQVYATQR*KC*SSMKEKNAQVVQ 323
+L GTSQN Q YATQR*+C S K+A VV+
Sbjct: 181 HPRLPSGTSQNVQGYATQR*RCWSFTGGKSAPVVR 285
>gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A185 5-PRIME, mRNA sequence
Length = 627
Score = 190 bits (409), Expect = 7e-048
Identities = 75/90 (83%), Positives = 79/90 (87%)
Frame = +2 / +1
Query: 110 NSFRMFSNEDVTIGSWMLAMNVNHENTHALCEPDCTESSIAVWDIPKCSGLCHPEVKMLE 289
NSFRMF+NEDVTIGSWMLAMNVNHENTHALCEP+CT SSIAVWDIPKCSGLCHPEVKMLE
Sbjct: 1 NSFRMFNNEDVTIGSWMLAMNVNHENTHALCEPECTASSIAVWDIPKCSGLCHPEVKMLE 180
Query: 290 LHERKECTGGPTVAAEVSESED**FLGWGT 379
LH RKECTGGPT AAE ++* LG T
Sbjct: 181 LHRRKECTGGPTEAAETETDDE*CLLGLET 270
Score = 141 bits (302), Expect = 4e-033
Identities = 61/89 (68%), Positives = 65/89 (73%)
Frame = -2 / -2
Query: 378 VPQPRNHQSSDSDTSAATVGPPVHXXXXXXXXXXXSGWHRPEHFGMSQTAIEDSVQSGSQ 199
V +P H SS SAA+VGPPVH SGWH PEHFGMSQTAIED+V SGSQ
Sbjct: 269 VSRPSKHHSSSVSVSAASVGPPVHSFRR*SSSIFTSGWHSPEHFGMSQTAIEDAVHSGSQ 90
Query: 198 SACVFSWLTFIASIHDPIVTSSLLNIRKL 112
A VFSWLTFIASI DP+VTSSLLNIRKL
Sbjct: 89 RAWVFSWLTFIASIQDPMVTSSLLNIRKL 3
Score = 137 bits (293), Expect = 7e-032
Identities = 54/89 (60%), Positives = 67/89 (75%)
Frame = +1 / +3
Query: 112 QFPYVQQRGRYNWIVDACYERQP*EHTCTL*T*LHGVFNCCLGHPKMLRSMPPRGKNARA 291
QFP VQQRGRY+WI+DACYERQP*EH C L T +HG+ +C LGHPKM R+MPPRGK+A A
Sbjct: 3 QFPNVQQRGRYHWILDACYERQP*EHPCPLRTRVHGILDCRLGHPKMFRAMPPRGKDAGA 182
Query: 292 P*KKRMHRWSNCGS*GVRV*GLMISWLGN 378
+R+HRWS+ G * +M +W G+
Sbjct: 183 SPAERVHRWSDGGGRNRNR*RVMFAWSGD 269
Score = 110 bits (234), Expect = 1e-023
Identities = 47/73 (64%), Positives = 55/73 (75%)
Frame = -3 / -3
Query: 329 PQLDHLCILFFHGALAFLPLGGIDLSILGCPKQQLKTPCSQVHKVHVCSHG*RS*QASTI 150
P DH C L A A LPLGGI L+ILGCP++Q + PC++V + H CSHG*RS*QAS I
Sbjct: 220 PPSDHRCTLSAGEAPASLPLGGIALNILGCPRRQSRMPCTRVRRGHGCSHG*RS*QASRI 41
Query: 149 QL*RPRC*TYGNC 111
Q *RPRC*T+GNC
Sbjct: 40 QW*RPRC*TFGNC 2
>gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009I22f, mRNA sequence
Length = 1066
Score = 103 bits (219), Expect(2) = 4e-041
Identities = 38/47 (80%), Positives = 41/47 (87%)
Frame = +2 / +3
Query: 140 VTIGSWMLAMNVNHENTHALCEPDCTESSIAVWDIPKCSGLCHPEVK 280
+TIGSWMLAMNVNHEN HALCEP+C SIA WDIPKCSGLCHP+ K
Sbjct: 216 LTIGSWMLAMNVNHENPHALCEPECNGFSIADWDIPKCSGLCHPKGK 356
Score = 91.3 bits (193), Expect(2) = 1e-036
Identities = 41/47 (87%), Positives = 43/47 (91%)
Frame = -2 / -2
Query: 144 VTSSLLNIRKLLFRNATKLATTSADKA*IGPYA*RKYCDPSKKDWGS 4
+TSSLLNIRKLLF ATKLATTSADKA*IGPYA*RKY DPS+KD GS
Sbjct: 219 LTSSLLNIRKLLFLRATKLATTSADKA*IGPYA*RKYSDPSRKDCGS 79
Score = 89.5 bits (189), Expect(2) = 2e-027
Identities = 36/47 (76%), Positives = 40/47 (85%)
Frame = -3 / -3
Query: 143 *RPRC*TYGNCCFAMQPS*PPRQLIKHKLAHMHEGSIVIRVKRIGAH 3
*RPRC*T+GNCCF+ QPS PP QLIKHK AHMHEGSI+I+V R AH
Sbjct: 218 *RPRC*TFGNCCFSEQPSLPPHQLIKHK*AHMHEGSILIQVGRTVAH 78
Score = 84.9 bits (179), Expect(2) = 4e-041
Identities = 34/49 (69%), Positives = 37/49 (75%)
Frame = +3 / +3
Query: 3 MSPNPFYSDHNTSFMHMGQFMLYQLTWWLAWLHCETTVSVCSATRTLQL 149
+S +P Y D NTSFMHMG FMLYQL WW AWL ETTVS CS TRTL +
Sbjct: 78 VSHSPSYLDQNTSFMHMGLFMLYQLMWWQAWLL*ETTVSECSTTRTLTI 224
Score = 81.7 bits (172), Expect(2) = 1e-036
Identities = 32/43 (74%), Positives = 36/43 (83%)
Frame = -2 / -1
Query: 270 GWHRPEHFGMSQTAIEDSVQSGSQSACVFSWLTFIASIHDPIV 142
GWH PEHFGMSQ+AIE+ + SGSQ A FSWLTFIASI DP+V
Sbjct: 346 GWHSPEHFGMSQSAIENPLHSGSQRAWGFSWLTFIASIQDPMV 218
Score = 79.4 bits (167), Expect(2) = 2e-024
Identities = 36/46 (78%), Positives = 40/46 (86%)
Frame = -1 / -1
Query: 142 NVLVAEHTETVVSQCNQASHHVS**SINWPICMKEVL*SE*KGLGL 5
NVLV EH+ETVVSQ NQA HH+S**SIN PICMKEV *S+*+GL L
Sbjct: 217 NVLVVEHSETVVSQSNQACHHIS**SINRPICMKEVF*SK*EGLWL 80
Score = 78.5 bits (165), Expect(2) = 5e-028
Identities = 31/46 (67%), Positives = 33/46 (71%)
Frame = +2 / +2
Query: 5 EPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDV 142
EPQSFLLGS+YFLHAYGPIY RNNSFRMF+NEDV
Sbjct: 80 EPQSFLLGSEYFLHAYGPIYALSADVVASLVALRNNSFRMFNNEDV 217
Score = 77.1 bits (162), Expect(2) = 1e-023
Identities = 35/46 (76%), Positives = 36/46 (78%)
Frame = +1 / +1
Query: 4 *APILFTRITILPSCIWANLCFIS*RGG*LGCIAKQQFPYVQQRGR 141
*A +L T I ILPSCIWA LCFIS* GG LGC KQQFP VQQRGR
Sbjct: 79 *ATVLPTWIRILPSCIWAYLCFIS*CGGKLGCSEKQQFPNVQQRGR 216
Score = 65.7 bits (137), Expect(2) = 5e-028
Identities = 24/43 (55%), Positives = 31/43 (72%)
Frame = +1 / +2
Query: 145 NWIVDACYERQP*EHTCTL*T*LHGVFNCCLGHPKMLRSMPPR 273
+WI+DACYERQP E C L + + +C LGHPKM R+MPP+
Sbjct: 221 HWILDACYERQPREPPCPLRARVQRILDCRLGHPKMFRAMPPQ 349
Score = 52.8 bits (109), Expect(2) = 2e-024
Identities = 24/41 (58%), Positives = 29/41 (70%)
Frame = -3 / -2
Query: 269 GGIDLSILGCPKQQLKTPCSQVHKVHVCSHG*RS*QASTIQ 147
GGI L+ILGCP +Q + C++ K H S G*RS*QAS IQ
Sbjct: 345 GGIALNILGCPSRQSRIRCTRARKGHGGSRG*RS*QASRIQ 223
Score = 52.8 bits (109), Expect(2) = 2e-027
Identities = 26/45 (57%), Positives = 28/45 (62%)
Frame = -1 / -3
Query: 280 FYLWVA*T*AFWDVPNSN*RLRAVRFTKCMCVLMVDVHSKHPRSN 146
F VA* * FWDVP N A+ K M VL+VDVHSKHP SN
Sbjct: 356 FTFGVA*P*TFWDVPVGNRESVALGLAKGMGVLVVDVHSKHPGSN 222
Score = 52.4 bits (108), Expect(2) = 1e-023
Identities = 26/45 (57%), Positives = 30/45 (66%)
Frame = +3 / +1
Query: 147 LDRGCLL*TSTMRTHMHFVNLTARSLQLLFGTSQNAQVYATQR*K 281
LD GCLL*TST RT M F + +A +L GTSQN Q YAT + K
Sbjct: 223 LDPGCLL*TSTTRTPMPFASPSATDSRLPTGTSQNVQGYATPKVK 357
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 321,917,320
Number of Sequences: 312970
Number of extensions: 5000207
Number of successful extensions: 39223
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7210840530
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)