TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3995470.2.1
(744 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ463250.1|BQ463250 HI04I09r HI Hordeum vulgare subsp. v... 95 1e-025
gb|BG310215.2|BG310215 HVSMEc0016H22f Hordeum vulgare seedl... 87 9e-024
gb|CA028410.1|CA028410 HZ61O22r HZ Hordeum vulgare subsp. v... 87 9e-024
gb|AV915714.1|AV915714 AV915714 K. Sato unpublished cDNA li... 87 9e-024
gb|BJ463901.1|BJ463901 BJ463901 K. Sato unpublished cDNA li... 87 9e-024
>gb|BQ463250.1|BQ463250 HI04I09r HI Hordeum vulgare subsp. vulgare cDNA clone HI04I09
5-PRIME, mRNA sequence
Length = 596
Score = 95.5 bits (202), Expect(2) = 1e-025
Identities = 40/48 (83%), Positives = 46/48 (95%)
Frame = +2 / +1
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR*L 340
K+LKETC+Y+KSLHREVDDLS+RLS LM+TMDN+SP AEIIRSLLR*L
Sbjct: 241 KMLKETCSYIKSLHREVDDLSDRLSDLMSTMDNNSPAAEIIRSLLR*L 384
Score = 40.9 bits (83), Expect(2) = 1e-025
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = +2 / +1
Query: 74 MSSGGRRGRISDDEINELISKLQALLP 154
MS RG +S++EINELIS+LQ LLP
Sbjct: 109 MSGRRSRGSVSEEEINELISRLQTLLP 189
>gb|BG310215.2|BG310215 HVSMEc0016H22f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0016H22f, mRNA sequence
Length = 845
Score = 86.8 bits (183), Expect(2) = 9e-024
Identities = 36/46 (78%), Positives = 43/46 (93%)
Frame = +2 / +1
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++S AEIIRS+LR
Sbjct: 331 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSAGAEIIRSILR 468
Score = 43.2 bits (88), Expect(2) = 9e-024
Identities = 18/21 (85%), Positives = 20/21 (95%)
Frame = +2 / +1
Query: 92 RGRISDDEINELISKLQALLP 154
RG ISD+EINELISKLQ+LLP
Sbjct: 229 RGAISDEEINELISKLQSLLP 291
>gb|CA028410.1|CA028410 HZ61O22r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ61O22
5-PRIME, mRNA sequence
Length = 616
Score = 86.8 bits (183), Expect(2) = 9e-024
Identities = 36/46 (78%), Positives = 43/46 (93%)
Frame = +2 / +2
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++S AEIIRS+LR
Sbjct: 338 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSAGAEIIRSILR 475
Score = 43.2 bits (88), Expect(2) = 9e-024
Identities = 18/21 (85%), Positives = 20/21 (95%)
Frame = +2 / +2
Query: 92 RGRISDDEINELISKLQALLP 154
RG ISD+EINELISKLQ+LLP
Sbjct: 236 RGAISDEEINELISKLQSLLP 298
>gb|AV915714.1|AV915714 AV915714 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15l14 5', mRNA sequence
Length = 603
Score = 86.8 bits (183), Expect(2) = 9e-024
Identities = 36/46 (78%), Positives = 43/46 (93%)
Frame = +2 / +1
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++S AEIIRS+LR
Sbjct: 376 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSAGAEIIRSILR 513
Score = 43.2 bits (88), Expect(2) = 9e-024
Identities = 18/21 (85%), Positives = 20/21 (95%)
Frame = +2 / +1
Query: 92 RGRISDDEINELISKLQALLP 154
RG ISD+EINELISKLQ+LLP
Sbjct: 274 RGAISDEEINELISKLQSLLP 336
>gb|BJ463901.1|BJ463901 BJ463901 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30p15 5', mRNA sequence
Length = 578
Score = 86.8 bits (183), Expect(2) = 9e-024
Identities = 36/46 (78%), Positives = 43/46 (93%)
Frame = +2 / +1
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++S AEIIRS+LR
Sbjct: 352 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSAGAEIIRSILR 489
Score = 43.2 bits (88), Expect(2) = 9e-024
Identities = 18/21 (85%), Positives = 20/21 (95%)
Frame = +2 / +1
Query: 92 RGRISDDEINELISKLQALLP 154
RG ISD+EINELISKLQ+LLP
Sbjct: 250 RGAISDEEINELISKLQSLLP 312
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 314,815,850
Number of Sequences: 312970
Number of extensions: 4290955
Number of successful extensions: 71929
Number of sequences better than 1.0e-020: 7
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8313674964
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)