TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3977955.2.1
(867 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 127 3e-056
gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA,... 127 4e-047
gb|BI956815.1|BI956815 HVSMEn0005H07f Hordeum vulgare rachi... 80 6e-029
gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthe... 122 3e-027
gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Li... 121 5e-027
gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA li... 118 5e-026
gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, wat... 118 5e-026
gb|BI956859.1|BI956859 HVSMEn0005L01f Hordeum vulgare rachi... 80 6e-024
gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA li... 103 2e-021
gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no... 100 8e-021
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 127 bits (271), Expect(2) = 3e-056
Identities = 48/82 (58%), Positives = 58/82 (70%)
Frame = -2 / +2
Query: 866 YQCTFDGYQDTLYTHAQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIIT 687
+ C FD +QDTLY HA+RQFFR+C ++GTIDFIFGNS V QNCLI R+PM NQ N +T
Sbjct: 32 FNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSVT 211
Query: 686 AQGRRDKRSVGGTVLHNCTIEP 621
A GR D G V+ NC + P
Sbjct: 212 AHGRTDPNMKSGLVIQNCRLVP 277
Score = 111 bits (238), Expect(2) = 3e-056
Identities = 40/76 (52%), Positives = 55/76 (72%)
Frame = -2 / +2
Query: 590 KIRTYLARPWKEYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFYAEVDNRGDGADMSK 411
KI +YL RPWKE+SR + +++ I FI P+G++ WNGDFGL+TL+YAE NRG GA SK
Sbjct: 305 KIPSYLGRPWKEFSRLVIMESQIADFIKPEGYMPWNGDFGLKTLYYAEYGNRGPGAGTSK 484
Query: 410 RAKWGGIKTVTYEEAQ 363
R W G + + +EA+
Sbjct: 485 RVTWPGFRVIARQEAE 532
Score = 90.0 bits (190), Expect(2) = 1e-028
Identities = 41/74 (55%), Positives = 45/74 (60%)
Frame = +1 / -3
Query: 622 GSMVQLCSTVPPTERLSRRPCAVMMLAWFAMGFRGWIRQFCSTTWELPKMKSMVPVTRQS 801
G+ Q T P S RPCAV W +MG R +RQF +T ELPKMKSMVP Q
Sbjct: 276 GTRRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPEMTQL 97
Query: 802 RKNCRCACVYSVSW 843
RKNCR AC YSVSW
Sbjct: 96 RKNCRRACTYSVSW 55
Score = 56.5 bits (117), Expect(2) = 1e-028
Identities = 30/61 (49%), Positives = 33/61 (54%)
Frame = +1 / -3
Query: 394 PPHLARLLMSAPSPRLSTSA*KSVSRPKSPFHSSQPLGSMKPPMSFWM*IVREYSFQGRA 573
P + LL+ AP PRL SA* SV PKSPFH P G MK + M RE SF G
Sbjct: 501 PGQVTLLLVPAPGPRLPYSA**SVLSPKSPFHGMYPSGLMKSAIWLSMMTSRENSFHGLP 322
Query: 574 R 576
R
Sbjct: 321 R 319
>gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_C24 5', mRNA sequence
Length = 602
Score = 127 bits (271), Expect(2) = 4e-047
Identities = 48/82 (58%), Positives = 58/82 (70%)
Frame = -2 / +3
Query: 866 YQCTFDGYQDTLYTHAQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIIT 687
+ C FD +QDTLY HA+RQFFR+C ++GTIDFIFGNS V QNCLI R+PM NQ N +T
Sbjct: 183 FNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIITRRPMDNQQNSVT 362
Query: 686 AQGRRDKRSVGGTVLHNCTIEP 621
A GR D G V+ NC + P
Sbjct: 363 AHGRTDPNMKSGLVIQNCRLVP 428
Score = 90.0 bits (190), Expect(2) = 1e-023
Identities = 41/74 (55%), Positives = 45/74 (60%)
Frame = +1 / -2
Query: 622 GSMVQLCSTVPPTERLSRRPCAVMMLAWFAMGFRGWIRQFCSTTWELPKMKSMVPVTRQS 801
G+ Q T P S RPCAV W +MG R +RQF +T ELPKMKSMVP Q
Sbjct: 427 GTRRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPEMTQL 248
Query: 802 RKNCRCACVYSVSW 843
RKNCR AC YSVSW
Sbjct: 247 RKNCRRACTYSVSW 206
Score = 81.7 bits (172), Expect(2) = 4e-047
Identities = 28/48 (58%), Positives = 39/48 (81%)
Frame = -2 / +3
Query: 590 KIRTYLARPWKEYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFYAE 447
KI +YL RPWKE+SR + +++ I FI P+G++ WNGDFGL+TL+YAE
Sbjct: 456 KIPSYLGRPWKEFSRLVIMESQIADFIKPEGYMPWNGDFGLKTLYYAE 599
Score = 40.0 bits (81), Expect(2) = 1e-023
Identities = 22/43 (51%), Positives = 23/43 (53%)
Frame = +1 / -2
Query: 448 SA*KSVSRPKSPFHSSQPLGSMKPPMSFWM*IVREYSFQGRAR 576
SA* SV PKSPFH P G MK + M RE SF G R
Sbjct: 598 SA**SVLSPKSPFHGMYPSGLMKSAIWLSMMTSRENSFHGLPR 470
>gb|BI956815.1|BI956815 HVSMEn0005H07f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005H07f, mRNA sequence
Length = 877
Score = 80.3 bits (169), Expect(3) = 6e-029
Identities = 29/70 (41%), Positives = 44/70 (62%)
Frame = -2 / +3
Query: 821 AQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIITAQGRRDKRSVGGTVL 642
+ RQF+R+C ++GT+DFI+GN++ V Q+CL+ R P+ + N+ITAQGR G
Sbjct: 6 SNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSF 185
Query: 641 HNCTIEPHPD 612
NC + D
Sbjct: 186 QNCRVTSQED 215
Score = 50.6 bits (104), Expect(3) = 6e-029
Identities = 14/38 (36%), Positives = 23/38 (60%)
Frame = -2 / +3
Query: 596 GGKIRTYLARPWKEYSRTIYIQNDIGGFIDPKGWLEWN 483
G + TY RPWK +S +++Q+ + + P GW+ WN
Sbjct: 222 GSETETYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAWN 335
Score = 34.5 bits (69), Expect(3) = 6e-029
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -2 / +3
Query: 464 TLFYAEVDNRGDGADMSKRAKW 399
T+FY E N G GA++SKR W
Sbjct: 360 TVFYGEYGNTGPGANLSKRVPW 425
>gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_L07 5', mRNA sequence
Length = 372
Score = 122 bits (261), Expect = 3e-027
Identities = 45/92 (48%), Positives = 59/92 (64%)
Frame = -2 / +2
Query: 866 YQCTFDGYQDTLYTHAQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIIT 687
Y+C F+G+QDTLY H+ RQF+RDCRV GT+DF+FGN+ V QNCL+ R P+ Q N +T
Sbjct: 89 YRCAFEGHQDTLYAHSLRQFYRDCRVAGTVDFVFGNAAAVFQNCLLLARAPLPGQKNSVT 268
Query: 686 AQGRRDKRSVGGTVLHNCTIEPHPDFKEEAGG 591
AQGR + G C + H D +A G
Sbjct: 269 AQGRFNASMNSGFRFQFCNVSAHDDLLRQANG 364
>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM022cC.01, mRNA sequence
Length = 1239
Score = 121 bits (259), Expect = 5e-027
Identities = 44/102 (43%), Positives = 63/102 (61%)
Frame = -2 / +3
Query: 584 RTYLARPWKEYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFYAEVDNRGDGADMSKRA 405
R+YL RPWK+YSRT+++Q+++ ++P GWL W+G F L+TL+Y E N G GA S R
Sbjct: 114 RSYLGRPWKQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRV 293
Query: 404 KWGGIKTVTYEEAQKEFTVETFIQGQQFIPKFGVPFIPGLLP 279
W G + +T FTV +FI G ++ +PF GL P
Sbjct: 294 TWKGYRVITSASEASTFTVGSFIDGDVWLAGTSIPFSAGL*P 419
>gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 5', mRNA sequence
Length = 528
Score = 118 bits (252), Expect = 5e-026
Identities = 44/80 (55%), Positives = 57/80 (71%)
Frame = -2 / +1
Query: 866 YQCTFDGYQDTLYTHAQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIIT 687
Y+C+F GYQDTLY H+ RQFFR+C + GTIDF+FGNS VLQ+C + R+P+ NQ+NI T
Sbjct: 283 YRCSFVGYQDTLYVHSLRQFFRECDIYGTIDFVFGNSAAVLQSCNLYARRPLPNQSNIYT 462
Query: 686 AQGRRDKRSVGGTVLHNCTI 627
AQGR D G + C +
Sbjct: 463 AQGRTDPNQNTGISIQKCKV 522
>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_C09 5', mRNA sequence
Length = 417
Score = 118 bits (252), Expect = 5e-026
Identities = 43/93 (46%), Positives = 58/93 (62%)
Frame = -2 / +2
Query: 563 WKEYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFYAEVDNRGDGADMSKRAKWGGIKT 384
WK YSRT+ +Q+D+ G +DP GW+ W+GDF L TL+YAE DN G G+D S+R W G
Sbjct: 2 WKMYSRTVIMQSDVAGIVDPAGWMPWSGDFALSTLYYAEYDNSGAGSDTSRRVNWPGYHV 181
Query: 383 VTYEEAQKEFTVETFIQGQQFIPKFGVPFIPGL 285
+ FTV + G ++P+ GVPF GL
Sbjct: 182 LNSTVDAGNFTVANMVLGDFWLPQTGVPFTVGL 280
>gb|BI956859.1|BI956859 HVSMEn0005L01f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005L01f, mRNA sequence
Length = 867
Score = 80.3 bits (169), Expect(2) = 6e-024
Identities = 29/70 (41%), Positives = 44/70 (62%)
Frame = -2 / +3
Query: 821 AQRQFFRDCRVTGTIDFIFGNSQVVLQNCLIQPRKPMANQANIITAQGRRDKRSVGGTVL 642
+ RQF+R+C ++GT+DFI+GN++ V Q+CL+ R P+ + N+ITAQGR G
Sbjct: 6 SNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSF 185
Query: 641 HNCTIEPHPD 612
NC + D
Sbjct: 186 QNCRVTSQED 215
Score = 50.6 bits (104), Expect(2) = 6e-024
Identities = 14/38 (36%), Positives = 23/38 (60%)
Frame = -2 / +3
Query: 596 GGKIRTYLARPWKEYSRTIYIQNDIGGFIDPKGWLEWN 483
G + TY RPWK +S +++Q+ + + P GW+ WN
Sbjct: 222 GSETETYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAWN 335
>gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 3', mRNA sequence
Length = 606
Score = 103 bits (219), Expect = 2e-021
Identities = 38/94 (40%), Positives = 56/94 (59%)
Frame = -2 / -3
Query: 560 KEYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFYAEVDNRGDGADMSKRAKWGGIKTV 381
K+YSRT+++Q+++ ++P GWL W+G F L+TL+Y E N G GA S R W G + +
Sbjct: 604 KQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVI 425
Query: 380 TYEEAQKEFTVETFIQGQQFIPKFGVPFIPGLLP 279
T FTV +FI G ++ +PF GL P
Sbjct: 424 TSASEASTFTVGSFIDGDVWLAGTSIPFSAGL*P 323
>gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ003_B10 5', mRNA sequence
Length = 449
Score = 100 bits (214), Expect = 8e-021
Identities = 37/93 (39%), Positives = 55/93 (59%)
Frame = -2 / +2
Query: 557 EYSRTIYIQNDIGGFIDPKGWLEWNGDFGLETLFYAEVDNRGDGADMSKRAKWGGIKTVT 378
+YSRT+++Q+++ ++P GWL W+G F L+TL+Y E N G GA S R W G + +T
Sbjct: 2 QYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVIT 181
Query: 377 YEEAQKEFTVETFIQGQQFIPKFGVPFIPGLLP 279
FTV +FI G ++ +PF GL P
Sbjct: 182 SASEASTFTVGSFIDGDVWLAGTSIPFSAGL*P 280
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 335,997,268
Number of Sequences: 312970
Number of extensions: 5857802
Number of successful extensions: 84238
Number of sequences better than 1.0e-020: 14
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 9936482404
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)