TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3923331.2.1
(496 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL500587.1|AL500587 AL500587 Hordeum vulgare Barke roots... 193 4e-049
gb|CA005005.1|CA005005 HU12K09u HU Hordeum vulgare subsp. v... 193 4e-049
gb|CA016096.1|CA016096 HV10C18u HV Hordeum vulgare subsp. v... 193 4e-049
gb|CA016336.1|CA016336 HV09G16u HV Hordeum vulgare subsp. v... 193 4e-049
gb|BM443222.2|BM443222 EBro02_SQ003_D03_R root, 3 week, hyd... 120 5e-027
>gb|AL500587.1|AL500587 AL500587 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW01C14u 3', mRNA sequence
Length = 526
Score = 193 bits (417), Expect = 4e-049
Identities = 80/113 (70%), Positives = 96/113 (84%)
Frame = -3 / -2
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+E+IY+RS QLTLGAL+SAV KF SL HVP+WKDERP SIRGGD+LK+Y+IY
Sbjct: 456 RERGRFKTENIYLRSDQLTLGALKSAVHDKFSSLKHVPIWKDERPSSIRGGDELKVYKIY 277
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*LLCPRLNVFPPILT 153
P+GLT+RQALY F+F DD+E+ +YIK HPCAKLEVIFV*L L+VF + T
Sbjct: 276 PIGLTERQALYKFQFSDDAEVARYIKGHPCAKLEVIFV*LQYCHLHVFLQLRT 118
Score = 127 bits (272), Expect = 4e-029
Identities = 67/132 (50%), Positives = 71/132 (53%)
Frame = +1 / +2
Query: 97 FATHNTLHYSTTETRTQSSVRIGGKTLSLGHSSYTKITSSFAQGWSLIYCXXXXXXLNLK 276
F TLH + T VR KT + SYTKITSSFA GW LIY LN
Sbjct: 62 FVLRLTLHKQESVTVHAELVRSCKKT*RWQYCSYTKITSSFAHGWPLIYLATSASSLN*N 241
Query: 277 PYKA*RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM 456
Y A R VRP G*ILY F FH GT LR+LNL TAD KAP+VN DL
Sbjct: 242 LYNACRSVRPIG*ILYTFNSSPPRILDGLSSFHIGTCLRELNLSCTADFKAPKVN*SDLR 421
Query: 457 *MLSDLNRPLSL 492
*M S LNRPLSL
Sbjct: 422 *MFSVLNRPLSL 457
Score = 109 bits (232), Expect = 1e-023
Identities = 55/108 (50%), Positives = 68/108 (62%)
Frame = +3 / +1
Query: 168 ENVKPRA**LHKNYFKLCTGMVFDILLEFRIIPKSKTVQSLTLR*TDWVNSV*FQVIPTS 347
++VK *L+KN+ KLCT M +I F I+ + K + L+ T W+N V Q IPTS
Sbjct: 133 KDVKMAIL*LYKNHLKLCTWMALNISSNFSIVTELKFI*RLSFCKTYWINFVHLQFIPTS 312
Query: 348 NARGPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLS 491
N PFILPYRN++EG KF H Q S S*L *S +N L LKPPSLS
Sbjct: 313 NT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPPSLS 456
>gb|CA005005.1|CA005005 HU12K09u HU Hordeum vulgare subsp. vulgare cDNA clone HU12K09
3-PRIME, mRNA sequence
Length = 543
Score = 193 bits (417), Expect = 4e-049
Identities = 80/113 (70%), Positives = 96/113 (84%)
Frame = -3 / -1
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+E+IY+RS QLTLGAL+SAV KF SL HVP+WKDERP SIRGGD+LK+Y+IY
Sbjct: 414 RERGRFKTENIYLRSDQLTLGALKSAVHDKFSSLKHVPIWKDERPSSIRGGDELKVYKIY 235
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*LLCPRLNVFPPILT 153
P+GLT+RQALY F+F DD+E+ +YIK HPCAKLEVIFV*L L+VF + T
Sbjct: 234 PIGLTERQALYKFQFSDDAEVARYIKGHPCAKLEVIFV*LQYCHLHVFLQLRT 76
Score = 127 bits (272), Expect = 4e-029
Identities = 67/132 (50%), Positives = 71/132 (53%)
Frame = +1 / +2
Query: 97 FATHNTLHYSTTETRTQSSVRIGGKTLSLGHSSYTKITSSFAQGWSLIYCXXXXXXLNLK 276
F TLH + T VR KT + SYTKITSSFA GW LIY LN
Sbjct: 20 FVLRLTLHKQESVTVHAELVRSCKKT*RWQYCSYTKITSSFAHGWPLIYLATSASSLN*N 199
Query: 277 PYKA*RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM 456
Y A R VRP G*ILY F FH GT LR+LNL TAD KAP+VN DL
Sbjct: 200 LYNACRSVRPIG*ILYTFNSSPPRILDGLSSFHIGTCLRELNLSCTADFKAPKVN*SDLR 379
Query: 457 *MLSDLNRPLSL 492
*M S LNRPLSL
Sbjct: 380 *MFSVLNRPLSL 415
Score = 109 bits (232), Expect = 1e-023
Identities = 55/108 (50%), Positives = 68/108 (62%)
Frame = +3 / +1
Query: 168 ENVKPRA**LHKNYFKLCTGMVFDILLEFRIIPKSKTVQSLTLR*TDWVNSV*FQVIPTS 347
++VK *L+KN+ KLCT M +I F I+ + K + L+ T W+N V Q IPTS
Sbjct: 91 KDVKMAIL*LYKNHLKLCTWMALNISSNFSIVTELKFI*RLSFCKTYWINFVHLQFIPTS 270
Query: 348 NARGPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLS 491
N PFILPYRN++EG KF H Q S S*L *S +N L LKPPSLS
Sbjct: 271 NT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPPSLS 414
>gb|CA016096.1|CA016096 HV10C18u HV Hordeum vulgare subsp. vulgare cDNA clone HV10C18
3-PRIME, mRNA sequence
Length = 556
Score = 193 bits (417), Expect = 4e-049
Identities = 80/113 (70%), Positives = 96/113 (84%)
Frame = -3 / -1
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+E+IY+RS QLTLGAL+SAV KF SL HVP+WKDERP SIRGGD+LK+Y+IY
Sbjct: 415 RERGRFKTENIYLRSDQLTLGALKSAVHDKFSSLKHVPIWKDERPSSIRGGDELKVYKIY 236
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*LLCPRLNVFPPILT 153
P+GLT+RQALY F+F DD+E+ +YIK HPCAKLEVIFV*L L+VF + T
Sbjct: 235 PIGLTERQALYKFQFSDDAEVARYIKGHPCAKLEVIFV*LQYCHLHVFLQLRT 77
Score = 127 bits (272), Expect = 4e-029
Identities = 67/132 (50%), Positives = 71/132 (53%)
Frame = +1 / +3
Query: 97 FATHNTLHYSTTETRTQSSVRIGGKTLSLGHSSYTKITSSFAQGWSLIYCXXXXXXLNLK 276
F TLH + T VR KT + SYTKITSSFA GW LIY LN
Sbjct: 21 FVLRLTLHKQESVTVHAELVRSCKKT*RWQYCSYTKITSSFAHGWPLIYLATSASSLN*N 200
Query: 277 PYKA*RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM 456
Y A R VRP G*ILY F FH GT LR+LNL TAD KAP+VN DL
Sbjct: 201 LYNACRSVRPIG*ILYTFNSSPPRILDGLSSFHIGTCLRELNLSCTADFKAPKVN*SDLR 380
Query: 457 *MLSDLNRPLSL 492
*M S LNRPLSL
Sbjct: 381 *MFSVLNRPLSL 416
Score = 109 bits (232), Expect = 1e-023
Identities = 55/108 (50%), Positives = 68/108 (62%)
Frame = +3 / +2
Query: 168 ENVKPRA**LHKNYFKLCTGMVFDILLEFRIIPKSKTVQSLTLR*TDWVNSV*FQVIPTS 347
++VK *L+KN+ KLCT M +I F I+ + K + L+ T W+N V Q IPTS
Sbjct: 92 KDVKMAIL*LYKNHLKLCTWMALNISSNFSIVTELKFI*RLSFCKTYWINFVHLQFIPTS 271
Query: 348 NARGPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLS 491
N PFILPYRN++EG KF H Q S S*L *S +N L LKPPSLS
Sbjct: 272 NT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPPSLS 415
>gb|CA016336.1|CA016336 HV09G16u HV Hordeum vulgare subsp. vulgare cDNA clone HV09G16
3-PRIME, mRNA sequence
Length = 540
Score = 193 bits (417), Expect = 4e-049
Identities = 80/113 (70%), Positives = 96/113 (84%)
Frame = -3 / -3
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+E+IY+RS QLTLGAL+SAV KF SL HVP+WKDERP SIRGGD+LK+Y+IY
Sbjct: 415 RERGRFKTENIYLRSDQLTLGALKSAVHDKFSSLKHVPIWKDERPSSIRGGDELKVYKIY 236
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*LLCPRLNVFPPILT 153
P+GLT+RQALY F+F DD+E+ +YIK HPCAKLEVIFV*L L+VF + T
Sbjct: 235 PIGLTERQALYKFQFSDDAEVARYIKGHPCAKLEVIFV*LQYCHLHVFLQLRT 77
Score = 127 bits (272), Expect = 4e-029
Identities = 67/132 (50%), Positives = 71/132 (53%)
Frame = +1 / +3
Query: 97 FATHNTLHYSTTETRTQSSVRIGGKTLSLGHSSYTKITSSFAQGWSLIYCXXXXXXLNLK 276
F TLH + T VR KT + SYTKITSSFA GW LIY LN
Sbjct: 21 FVLRLTLHKQESVTVHAELVRSCKKT*RWQYCSYTKITSSFAHGWPLIYLATSASSLN*N 200
Query: 277 PYKA*RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM 456
Y A R VRP G*ILY F FH GT LR+LNL TAD KAP+VN DL
Sbjct: 201 LYNACRSVRPIG*ILYTFNSSPPRILDGLSSFHIGTCLRELNLSCTADFKAPKVN*SDLR 380
Query: 457 *MLSDLNRPLSL 492
*M S LNRPLSL
Sbjct: 381 *MFSVLNRPLSL 416
Score = 109 bits (232), Expect = 1e-023
Identities = 55/108 (50%), Positives = 68/108 (62%)
Frame = +3 / +2
Query: 168 ENVKPRA**LHKNYFKLCTGMVFDILLEFRIIPKSKTVQSLTLR*TDWVNSV*FQVIPTS 347
++VK *L+KN+ KLCT M +I F I+ + K + L+ T W+N V Q IPTS
Sbjct: 92 KDVKMAIL*LYKNHLKLCTWMALNISSNFSIVTELKFI*RLSFCKTYWINFVHLQFIPTS 271
Query: 348 NARGPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLS 491
N PFILPYRN++EG KF H Q S S*L *S +N L LKPPSLS
Sbjct: 272 NT*WPFILPYRNMLEGAKFIVHSRLQSS*S*LI*S*INVLCLKPPSLS 415
>gb|BM443222.2|BM443222 EBro02_SQ003_D03_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ003_D03 5', mRNA sequence
Length = 383
Score = 120 bits (257), Expect = 5e-027
Identities = 47/62 (75%), Positives = 56/62 (90%)
Frame = -3 / +3
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+E+IY+RS QLTLGAL+SAV KF SL HVP+WKDERP SIRGGD+LK+Y+IY
Sbjct: 195 RERGRFKTENIYLRSDQLTLGALKSAVHDKFSSLKHVPIWKDERPSSIRGGDELKVYKIY 374
Query: 311 PV 306
P+
Sbjct: 375 PI 380
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 220,140,905
Number of Sequences: 312970
Number of extensions: 2913850
Number of successful extensions: 42142
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 4871183406
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)