TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3848939.2.1
(952 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF265428.3|BF265428 HV_CEa0012D19f Hordeum vulgare seedl... 85 8e-035
gb|BE421032.1|BE421032 HWM005.B01 ITEC HWM Barley Leaf Libr... 85 2e-034
gb|BJ455149.1|BJ455149 BJ455149 K. Sato unpublished cDNA li... 83 4e-031
gb|BJ447398.1|BJ447398 BJ447398 K. Sato unpublished cDNA li... 83 4e-031
gb|AV911012.1|AV911012 AV911012 K. Sato unpublished cDNA li... 83 2e-029
gb|BJ452082.1|BJ452082 BJ452082 K. Sato unpublished cDNA li... 83 2e-029
gb|AV924239.1|AV924239 AV924239 K. Sato unpublished cDNA li... 83 3e-029
gb|BJ454629.1|BJ454629 BJ454629 K. Sato unpublished cDNA li... 83 3e-029
gb|AV909129.1|AV909129 AV909129 K. Sato unpublished cDNA li... 83 3e-029
gb|BJ463507.1|BJ463507 BJ463507 K. Sato unpublished cDNA li... 83 1e-028
gb|BJ466456.1|BJ466456 BJ466456 K. Sato unpublished cDNA li... 80 2e-028
gb|AV930309.1|AV930309 AV930309 K. Sato unpublished cDNA li... 79 3e-028
gb|BF623113.2|BF623113 HVSMEa0014H10f Hordeum vulgare seedl... 80 3e-027
gb|AV930310.1|AV930310 AV930310 K. Sato unpublished cDNA li... 80 2e-026
gb|BJ456207.1|BJ456207 BJ456207 K. Sato unpublished cDNA li... 83 1e-025
gb|BJ447126.1|BJ447126 BJ447126 K. Sato unpublished cDNA li... 68 2e-024
gb|AV925157.1|AV925157 AV925157 K. Sato unpublished cDNA li... 63 2e-022
gb|BJ455637.1|BJ455637 BJ455637 K. Sato unpublished cDNA li... 83 5e-022
gb|BJ460586.1|BJ460586 BJ460586 K. Sato unpublished cDNA li... 83 5e-022
gb|BG299400.1|BG299400 HVSMEa0019M24f Hordeum vulgare seedl... 64 3e-021
gb|BJ448486.1|BJ448486 BJ448486 K. Sato unpublished cDNA li... 83 4e-021
gb|BF264237.3|BF264237 HV_CEa0008O05f Hordeum vulgare seedl... 82 6e-021
>gb|BF265428.3|BF265428 HV_CEa0012D19f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0012D19f, mRNA sequence
Length = 827
Score = 84.9 bits (179), Expect(2) = 8e-035
Identities = 32/38 (84%), Positives = 35/38 (92%)
Frame = +2 / +1
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCPVD LKL+ACVDALNG+ HAVVG NAS+TCCPLLS
Sbjct: 277 GKCPVDVLKLVACVDALNGVVHAVVGANASETCCPLLS 390
Score = 82.6 bits (174), Expect(2) = 8e-035
Identities = 34/36 (94%), Positives = 36/36 (100%)
Frame = +1 / +1
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIKAKAL+VSLVLPVAISVLVN+CGKHVPSSFQCP
Sbjct: 427 TTIKAKALNVSLVLPVAISVLVNQCGKHVPSSFQCP 534
Score = 75.3 bits (158), Expect(3) = 2e-025
Identities = 33/38 (86%), Positives = 34/38 (89%)
Frame = -3 / -1
Query: 506 YYGHWKLEGTCLPHSLTSTEMATGSTRLTLSALALMVV 393
Y GH K +GTCLPH LTSTEMATGSTRLTLSALALMVV
Sbjct: 539 YEGH*KEDGTCLPHWLTSTEMATGSTRLTLSALALMVV 426
Score = 54.7 bits (113), Expect(3) = 2e-025
Identities = 23/38 (60%), Positives = 23/38 (60%)
Frame = -2 / -1
Query: 357 DSSGQQVSLALVPTTACARPLXXXXXXXXXXXXTGHLP 244
DSSGQQVSLAL PTTAC PL TGHLP
Sbjct: 389 DSSGQQVSLALAPTTACTTPLSASTHATSFSTSTGHLP 276
Score = 24.0 bits (46), Expect(3) = 2e-025
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = -3 / -2
Query: 254 GTCPSAWTAS 225
GTCPSA TAS
Sbjct: 286 GTCPSATTAS 257
>gb|BE421032.1|BE421032 HWM005.B01 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM005.B01, mRNA sequence
Length = 1148
Score = 84.9 bits (179), Expect(2) = 2e-034
Identities = 32/38 (84%), Positives = 35/38 (92%)
Frame = +2 / +1
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCPVD LKL+ACVDALNG+ HAVVG NAS+TCCPLLS
Sbjct: 145 GKCPVDVLKLVACVDALNGVVHAVVGANASETCCPLLS 258
Score = 81.3 bits (171), Expect(2) = 2e-034
Identities = 33/36 (91%), Positives = 36/36 (100%)
Frame = +1 / +1
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIKAKAL+VSLVLPVAISVLVN+CGKHVP+SFQCP
Sbjct: 295 TTIKAKALNVSLVLPVAISVLVNQCGKHVPASFQCP 402
Score = 73.9 bits (155), Expect(2) = 3e-023
Identities = 33/38 (86%), Positives = 33/38 (86%)
Frame = -3 / -1
Query: 506 YYGHWKLEGTCLPHSLTSTEMATGSTRLTLSALALMVV 393
Y GH K GTCLPH LTSTEMATGSTRLTLSALALMVV
Sbjct: 407 YEGH*KEAGTCLPHWLTSTEMATGSTRLTLSALALMVV 294
Score = 54.7 bits (113), Expect(2) = 3e-023
Identities = 23/38 (60%), Positives = 23/38 (60%)
Frame = -2 / -1
Query: 357 DSSGQQVSLALVPTTACARPLXXXXXXXXXXXXTGHLP 244
DSSGQQVSLAL PTTAC PL TGHLP
Sbjct: 257 DSSGQQVSLALAPTTACTTPLSASTHATSFSTSTGHLP 144
>gb|BJ455149.1|BJ455149 BJ455149 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak35f14 3', mRNA sequence
Length = 564
Score = 82.6 bits (174), Expect(2) = 4e-031
Identities = 34/36 (94%), Positives = 36/36 (100%)
Frame = +1 / -3
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIKAKAL+VSLVLPVAISVLVN+CGKHVPSSFQCP
Sbjct: 424 TTIKAKALNVSLVLPVAISVLVNQCGKHVPSSFQCP 317
Score = 82.2 bits (173), Expect(3) = 3e-025
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = -3 / +3
Query: 506 YYGHWKLEGTCLPHSLTSTEMATGSTRLTLSALALMVV 393
Y GHWK +GTCLPH LTSTEMATGSTRLTLSALALMVV
Sbjct: 312 YEGHWKEDGTCLPHWLTSTEMATGSTRLTLSALALMVV 425
Score = 72.5 bits (152), Expect(2) = 4e-031
Identities = 28/34 (82%), Positives = 31/34 (91%)
Frame = +2 / -3
Query: 257 VDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
VD LKL+ACVDALNG+ HAVVG NAS+TCCPLLS
Sbjct: 562 VDVLKLVACVDALNGVVHAVVGANASETCCPLLS 461
Score = 44.1 bits (90), Expect(3) = 3e-025
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -2 / +3
Query: 357 DSSGQQVSLALVPTTACARPL 295
DSSGQQVSLAL PTTAC PL
Sbjct: 462 DSSGQQVSLALAPTTACTTPL 524
Score = 26.7 bits (52), Expect(3) = 3e-025
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = -1 / +2
Query: 310 VREAVERVHARQQLQ 266
V VERVHAR QLQ
Sbjct: 509 VHHPVERVHARHQLQ 553
>gb|BJ447398.1|BJ447398 BJ447398 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak35f14 5', mRNA sequence
Length = 540
Score = 82.6 bits (174), Expect(2) = 4e-031
Identities = 34/36 (94%), Positives = 36/36 (100%)
Frame = +1 / +1
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIKAKAL+VSLVLPVAISVLVN+CGKHVPSSFQCP
Sbjct: 151 TTIKAKALNVSLVLPVAISVLVNQCGKHVPSSFQCP 258
Score = 82.2 bits (173), Expect(3) = 3e-025
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = -3 / -2
Query: 506 YYGHWKLEGTCLPHSLTSTEMATGSTRLTLSALALMVV 393
Y GHWK +GTCLPH LTSTEMATGSTRLTLSALALMVV
Sbjct: 263 YEGHWKEDGTCLPHWLTSTEMATGSTRLTLSALALMVV 150
Score = 72.5 bits (152), Expect(2) = 4e-031
Identities = 28/34 (82%), Positives = 31/34 (91%)
Frame = +2 / +1
Query: 257 VDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
VD LKL+ACVDALNG+ HAVVG NAS+TCCPLLS
Sbjct: 13 VDVLKLVACVDALNGVVHAVVGANASETCCPLLS 114
Score = 44.1 bits (90), Expect(3) = 3e-025
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -2 / -2
Query: 357 DSSGQQVSLALVPTTACARPL 295
DSSGQQVSLAL PTTAC PL
Sbjct: 113 DSSGQQVSLALAPTTACTTPL 51
Score = 26.7 bits (52), Expect(3) = 3e-025
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = -1 / -1
Query: 310 VREAVERVHARQQLQ 266
V VERVHAR QLQ
Sbjct: 66 VHHPVERVHARHQLQ 22
>gb|AV911012.1|AV911012 AV911012 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11l03 3', mRNA sequence
Length = 580
Score = 82.6 bits (174), Expect(3) = 2e-029
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +2 / -3
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCP+DTLKLL CVDAL+GL HA++G+NASD CCPLLS
Sbjct: 527 GKCPIDTLKLLGCVDALDGLLHALIGSNASDKCCPLLS 414
Score = 66.1 bits (138), Expect(3) = 2e-029
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / -3
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL+++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 377 TTIKLKALNINLVLPIAIEVLVNQCGKTVPDDFKCP 270
Score = 21.7 bits (41), Expect(3) = 2e-029
Identities = 6/9 (66%), Positives = 6/9 (66%)
Frame = +2 / -1
Query: 719 WSCGGCAFF 745
WSCG C F
Sbjct: 136 WSCGTCMHF 110
>gb|BJ452082.1|BJ452082 BJ452082 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak37l03 5', mRNA sequence
Length = 637
Score = 82.6 bits (174), Expect(3) = 2e-029
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +2 / +1
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCP+DTLKLL CVDAL+GL HA++G+NASD CCPLLS
Sbjct: 220 GKCPIDTLKLLGCVDALDGLLHALIGSNASDKCCPLLS 333
Score = 66.1 bits (138), Expect(3) = 2e-029
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / +1
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL+++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 370 TTIKLKALNINLVLPIAIEVLVNQCGKTVPDDFKCP 477
Score = 21.7 bits (41), Expect(3) = 2e-029
Identities = 6/9 (66%), Positives = 6/9 (66%)
Frame = +2 / +2
Query: 719 WSCGGCAFF 745
WSCG C F
Sbjct: 611 WSCGTCMHF 637
>gb|AV924239.1|AV924239 AV924239 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd11n13 5', mRNA sequence
Length = 685
Score = 82.6 bits (174), Expect(3) = 3e-029
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +2 / +3
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCP+DTLKLL CVDAL+GL HA++G+NASD CCPLLS
Sbjct: 213 GKCPIDTLKLLGCVDALDGLLHALIGSNASDKCCPLLS 326
Score = 66.1 bits (138), Expect(3) = 3e-029
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / +3
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL+++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 363 TTIKLKALNINLVLPIAIEVLVNQCGKTVPDDFKCP 470
Score = 21.7 bits (41), Expect(3) = 3e-029
Identities = 6/9 (66%), Positives = 6/9 (66%)
Frame = +2 / +1
Query: 719 WSCGGCAFF 745
WSCG C F
Sbjct: 604 WSCGTCMHF 630
>gb|BJ454629.1|BJ454629 BJ454629 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak14c15 3', mRNA sequence
Length = 866
Score = 82.6 bits (174), Expect(2) = 3e-029
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +2 / -1
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCP+DTLKLL CVDAL+GL HA++G+NASD CCPLLS
Sbjct: 452 GKCPIDTLKLLGCVDALDGLLHALIGSNASDKCCPLLS 339
Score = 66.1 bits (138), Expect(2) = 3e-029
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / -1
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL+++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 302 TTIKLKALNINLVLPIAIEVLVNQCGKTVPDDFKCP 195
>gb|AV909129.1|AV909129 AV909129 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11l03 5', mRNA sequence
Length = 629
Score = 82.6 bits (174), Expect(2) = 3e-029
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +2 / +3
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCP+DTLKLL CVDAL+GL HA++G+NASD CCPLLS
Sbjct: 213 GKCPIDTLKLLGCVDALDGLLHALIGSNASDKCCPLLS 326
Score = 66.1 bits (138), Expect(2) = 3e-029
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / +3
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL+++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 363 TTIKLKALNINLVLPIAIEVLVNQCGKTVPDDFKCP 470
>gb|BJ463507.1|BJ463507 BJ463507 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32d05 5', mRNA sequence
Length = 734
Score = 82.6 bits (174), Expect(2) = 1e-028
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +2 / +1
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCPVDTL+LL CVDALNGL H V+G++ASD+CCPLLS
Sbjct: 466 GKCPVDTLRLLGCVDALNGLVHTVIGSSASDSCCPLLS 579
Score = 64.3 bits (134), Expect(2) = 1e-028
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / +1
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTI+ KAL+++LVL +AI VLVN+CGK VPS FQCP
Sbjct: 616 TTIELKALNINLVLSIAIQVLVNQCGKTVPSDFQCP 723
>gb|BJ466456.1|BJ466456 BJ466456 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32d05 3', mRNA sequence
Length = 866
Score = 79.9 bits (168), Expect(2) = 2e-028
Identities = 30/38 (78%), Positives = 34/38 (89%)
Frame = +2 / -2
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCP DTLKLL CVDAL+GL HA++G+NASD CCPLLS
Sbjct: 520 GKCPHDTLKLLGCVDALDGLLHALIGSNASDKCCPLLS 407
Score = 66.1 bits (138), Expect(2) = 2e-028
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / -2
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL+++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 370 TTIKLKALNINLVLPIAIEVLVNQCGKTVPDDFKCP 263
>gb|AV930309.1|AV930309 AV930309 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd21p03 3', mRNA sequence
Length = 748
Score = 79.4 bits (167), Expect(2) = 3e-028
Identities = 29/38 (76%), Positives = 34/38 (89%)
Frame = +2 / -3
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCP+DTLKLL CV AL+GL HA++G+NASD CCPLLS
Sbjct: 374 GKCPIDTLKLLGCVXALDGLLHALIGSNASDKCCPLLS 261
Score = 66.1 bits (138), Expect(2) = 3e-028
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / -3
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL+++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 224 TTIKLKALNINLVLPIAIEVLVNQCGKTVPDDFKCP 117
>gb|BF623113.2|BF623113 HVSMEa0014H10f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0014H10f, mRNA sequence
Length = 878
Score = 80.3 bits (169), Expect(2) = 3e-027
Identities = 30/38 (78%), Positives = 34/38 (89%)
Frame = +2 / -2
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCPV TLKLL CVDALNGL H V+G++ASDTCCPLL+
Sbjct: 271 GKCPVYTLKLLGCVDALNGLVHTVIGSSASDTCCPLLA 158
Score = 61.6 bits (128), Expect(2) = 3e-027
Identities = 25/35 (71%), Positives = 30/35 (85%)
Frame = +1 / -2
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQC 498
TTIK KAL+++LVLP+AI VLVN+ GK VPS FQC
Sbjct: 121 TTIKLKALNINLVLPIAIQVLVNQYGKTVPSDFQC 17
>gb|AV930310.1|AV930310 AV930310 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd21p04 3', mRNA sequence
Length = 685
Score = 79.9 bits (168), Expect(2) = 2e-026
Identities = 29/38 (76%), Positives = 34/38 (89%)
Frame = +2 / -2
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCP+DTL LL CVDAL+GL HA++G+NASD CCPLLS
Sbjct: 375 GKCPIDTLXLLGCVDALDGLLHALIGSNASDKCCPLLS 262
Score = 59.3 bits (123), Expect(2) = 2e-026
Identities = 25/37 (67%), Positives = 29/37 (78%)
Frame = +1 / -2
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP* 504
TTIK KAL+++LVLP AI VLVN+CGK VP F P*
Sbjct: 225 TTIKLKALNINLVLPXAIEVLVNQCGKTVPDDFTVP* 115
>gb|BJ456207.1|BJ456207 BJ456207 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak20c05 3', mRNA sequence
Length = 488
Score = 82.6 bits (174), Expect(2) = 1e-025
Identities = 34/36 (94%), Positives = 36/36 (100%)
Frame = +1 / -1
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIKAKAL+VSLVLPVAISVLVN+CGKHVPSSFQCP
Sbjct: 380 TTIKAKALNVSLVLPVAISVLVNQCGKHVPSSFQCP 273
Score = 82.2 bits (173), Expect(2) = 1e-022
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = -3 / +1
Query: 506 YYGHWKLEGTCLPHSLTSTEMATGSTRLTLSALALMVV 393
Y GHWK +GTCLPH LTSTEMATGSTRLTLSALALMVV
Sbjct: 268 YEGHWKEDGTCLPHWLTSTEMATGSTRLTLSALALMVV 381
Score = 53.8 bits (111), Expect(2) = 1e-025
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = +2 / -1
Query: 287 DALNGLAHAVVGTNASDTCCPLLS 358
DALNG+ HAVVG NAS+TCCPLLS
Sbjct: 488 DALNGVVHAVVGANASETCCPLLS 417
Score = 44.1 bits (90), Expect(2) = 1e-022
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -2 / +1
Query: 357 DSSGQQVSLALVPTTACARPL 295
DSSGQQVSLAL PTTAC PL
Sbjct: 418 DSSGQQVSLALAPTTACTTPL 480
>gb|BJ447126.1|BJ447126 BJ447126 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak14c15 5', mRNA sequence
Length = 682
Score = 68.0 bits (142), Expect(2) = 2e-024
Identities = 26/36 (72%), Positives = 32/36 (88%)
Frame = +1 / +1
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTI+ KAL+++LVLP+AI VLVN+CGK VPS FQCP
Sbjct: 133 TTIELKALNINLVLPIAIQVLVNQCGKTVPSDFQCP 240
Score = 66.1 bits (138), Expect(3) = 2e-024
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +1 / +2
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL+++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 575 TTIKLKALNINLVLPIAIEVLVNQCGKTVPDDFKCP 682
Score = 64.8 bits (135), Expect(2) = 2e-024
Identities = 24/31 (77%), Positives = 28/31 (90%)
Frame = +2 / +1
Query: 266 LKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
LKLL CVDALNGL H V+G++ASD+CCPLLS
Sbjct: 4 LKLLGCVDALNGLVHTVIGSSASDSCCPLLS 96
Score = 62.0 bits (129), Expect(3) = 2e-024
Identities = 23/35 (65%), Positives = 28/35 (80%)
Frame = +2 / +1
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCP 349
GKCP+DTLKLL CVDAL+GL HA++G+NA P
Sbjct: 424 GKCPIDTLKLLGCVDALDGLLHALIGSNARRQMLP 528
Score = 22.1 bits (42), Expect(3) = 2e-024
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = +2 / +2
Query: 335 DTCCPLLS 358
D CCPLLS
Sbjct: 515 DKCCPLLS 538
>gb|AV925157.1|AV925157 AV925157 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd21p04 5', mRNA sequence
Length = 563
Score = 62.9 bits (131), Expect(3) = 2e-022
Identities = 24/36 (66%), Positives = 29/36 (80%)
Frame = +1 / +3
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KAL ++LVLP+AI VLVN+CGK P F+CP
Sbjct: 456 TTIKLKALXINLVLPIAIEVLVNQCGKTEPDDFKCP 563
Score = 50.6 bits (104), Expect(3) = 2e-022
Identities = 18/25 (72%), Positives = 22/25 (88%)
Frame = +2 / +2
Query: 245 GKCPVDTLKLLACVDALNGLAHAVV 319
GKCP+ TLKLL CVDAL+GL HA++
Sbjct: 305 GKCPIXTLKLLGCVDALDGLLHALI 379
Score = 29.9 bits (59), Expect(3) = 2e-022
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +2 / +3
Query: 320 GTNASDTCCPLL 355
G+NASD CCPLL
Sbjct: 381 GSNASDKCCPLL 416
>gb|BJ455637.1|BJ455637 BJ455637 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17k09 3', mRNA sequence
Length = 477
Score = 82.6 bits (174), Expect(2) = 5e-022
Identities = 34/36 (94%), Positives = 36/36 (100%)
Frame = +1 / -2
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIKAKAL+VSLVLPVAISVLVN+CGKHVPSSFQCP
Sbjct: 383 TTIKAKALNVSLVLPVAISVLVNQCGKHVPSSFQCP 276
Score = 82.2 bits (173), Expect(2) = 3e-021
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = -3 / +1
Query: 506 YYGHWKLEGTCLPHSLTSTEMATGSTRLTLSALALMVV 393
Y GHWK +GTCLPH LTSTEMATGSTRLTLSALALMVV
Sbjct: 271 YEGHWKEDGTCLPHWLTSTEMATGSTRLTLSALALMVV 384
Score = 41.8 bits (85), Expect(2) = 5e-022
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +2 / -2
Query: 302 LAHAVVGTNASDTCCPLLS 358
+ HAVVG NAS+TCCPLLS
Sbjct: 476 VVHAVVGANASETCCPLLS 420
Score = 39.6 bits (80), Expect(2) = 3e-021
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = -2 / +1
Query: 357 DSSGQQVSLALVPTTAC 307
DSSGQQVSLAL PTTAC
Sbjct: 421 DSSGQQVSLALAPTTAC 471
>gb|BJ460586.1|BJ460586 BJ460586 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41g13 3', mRNA sequence
Length = 477
Score = 82.6 bits (174), Expect(2) = 5e-022
Identities = 34/36 (94%), Positives = 36/36 (100%)
Frame = +1 / -2
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIKAKAL+VSLVLPVAISVLVN+CGKHVPSSFQCP
Sbjct: 383 TTIKAKALNVSLVLPVAISVLVNQCGKHVPSSFQCP 276
Score = 82.2 bits (173), Expect(2) = 3e-021
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = -3 / +1
Query: 506 YYGHWKLEGTCLPHSLTSTEMATGSTRLTLSALALMVV 393
Y GHWK +GTCLPH LTSTEMATGSTRLTLSALALMVV
Sbjct: 271 YEGHWKEDGTCLPHWLTSTEMATGSTRLTLSALALMVV 384
Score = 41.8 bits (85), Expect(2) = 5e-022
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +2 / -2
Query: 302 LAHAVVGTNASDTCCPLLS 358
+ HAVVG NAS+TCCPLLS
Sbjct: 476 VVHAVVGANASETCCPLLS 420
Score = 39.6 bits (80), Expect(2) = 3e-021
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = -2 / +1
Query: 357 DSSGQQVSLALVPTTAC 307
DSSGQQVSLAL PTTAC
Sbjct: 421 DSSGQQVSLALAPTTAC 471
>gb|BG299400.1|BG299400 HVSMEa0019M24f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0019M24f, mRNA sequence
Length = 633
Score = 64.3 bits (134), Expect(3) = 3e-021
Identities = 24/36 (66%), Positives = 30/36 (83%)
Frame = +1 / -2
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIK KA +++LVLP+AI VLVN+CGK VP F+CP
Sbjct: 419 TTIKLKAFNINLVLPIAIEVLVNQCGKTVPDDFKCP 312
Score = 57.0 bits (118), Expect(3) = 3e-021
Identities = 22/35 (62%), Positives = 26/35 (74%)
Frame = +2 / -1
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCP 349
GKCP+DTLKLL CVDAL L HA++ NA+D P
Sbjct: 570 GKCPIDTLKLLGCVDALYELRHALMANNATDKWLP 466
Score = 21.7 bits (41), Expect(3) = 3e-021
Identities = 6/9 (66%), Positives = 6/9 (66%)
Frame = +2 / -3
Query: 719 WSCGGCAFF 745
WSCG C F
Sbjct: 178 WSCGTCMHF 152
>gb|BJ448486.1|BJ448486 BJ448486 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak20c05 5', mRNA sequence
Length = 504
Score = 82.6 bits (174), Expect(3) = 4e-021
Identities = 34/36 (94%), Positives = 36/36 (100%)
Frame = +1 / +3
Query: 394 TTIKAKALSVSLVLPVAISVLVNECGKHVPSSFQCP 501
TTIKAKAL+VSLVLPVAISVLVN+CGKHVPSSFQCP
Sbjct: 111 TTIKAKALNVSLVLPVAISVLVNQCGKHVPSSFQCP 218
Score = 34.1 bits (68), Expect(3) = 4e-021
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +2 / +2
Query: 290 ALNGLAHAVVGTNASDTCCP 349
ALNG+ HAVVG NAS+ P
Sbjct: 5 ALNGVVHAVVGANASEXLLP 64
Score = 22.1 bits (42), Expect(3) = 4e-021
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +2 / +3
Query: 338 TCCPLLS 358
TCCPLLS
Sbjct: 54 TCCPLLS 74
>gb|BF264237.3|BF264237 HV_CEa0008O05f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0008O05f, mRNA sequence
Length = 611
Score = 81.7 bits (172), Expect(3) = 6e-021
Identities = 31/38 (81%), Positives = 34/38 (89%)
Frame = +2 / +2
Query: 245 GKCPVDTLKLLACVDALNGLAHAVVGTNASDTCCPLLS 358
GKCPVD LKL+ACVDALNG+ HA VG NAS+TCCPLLS
Sbjct: 272 GKCPVDVLKLVACVDALNGVVHAGVGANASETCCPLLS 385
Score = 28.6 bits (56), Expect(3) = 6e-021
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +1 / +1
Query: 400 IKAKALSVSLVLPVA 444
+K KAL+VSLVLPVA
Sbjct: 427 LKGKALNVSLVLPVA 471
Score = 28.1 bits (55), Expect(3) = 6e-021
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1 / +3
Query: 436 PVAISVLVNECGKHVPSSFQ 495
P ++ VLVN+ KHVP SF+
Sbjct: 462 PRSLLVLVNQWEKHVPCSFR 521
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 311,421,197
Number of Sequences: 312970
Number of extensions: 3925551
Number of successful extensions: 35144
Number of sequences better than 1.0e-020: 35
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 11115387096
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)