TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3829259.2.1
(1019 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE558753.3|BE558753 HV_CEb0020E08f Hordeum vulgare seedl... 240 e-111
gb|BJ463662.1|BJ463662 BJ463662 K. Sato unpublished cDNA li... 211 3e-092
gb|CA013313.1|CA013313 HT08A11r HT Hordeum vulgare subsp. v... 200 1e-081
gb|BE420919.1|BE420919 HWM003.H08 ITEC HWM Barley Leaf Libr... 177 5e-081
gb|CA013080.1|CA013080 HT07F09r HT Hordeum vulgare subsp. v... 153 1e-075
gb|BU990717.1|BU990717 HF25N16r HF Hordeum vulgare subsp. v... 200 6e-071
gb|BU971800.1|BU971800 HB19L11r BC Hordeum vulgare subsp. v... 152 4e-063
gb|BU995681.1|BU995681 HM11A08r HM Hordeum vulgare subsp. v... 200 8e-063
gb|BM371846.2|BM371846 EBma08_SQ004_C16_R maternal, 28 DPA,... 133 9e-062
gb|BJ449925.1|BJ449925 BJ449925 K. Sato unpublished cDNA li... 197 7e-060
gb|AV936911.1|AV936911 AV936911 K. Sato unpublished cDNA li... 198 1e-056
gb|BQ766274.1|BQ766274 EBro08_SQ005_C17_R root, 3 week, dro... 180 5e-055
gb|BJ466608.1|BJ466608 BJ466608 K. Sato unpublished cDNA li... 171 3e-052
gb|BJ457343.1|BJ457343 BJ457343 K. Sato unpublished cDNA li... 160 3e-049
gb|CA021085.1|CA021085 HZ39B01r HZ Hordeum vulgare subsp. v... 186 2e-046
gb|BI952869.1|BI952869 HVSMEm0008B04f Hordeum vulgare green... 127 4e-041
gb|CA000191.1|CA000191 HS09J09u HS Hordeum vulgare subsp. v... 153 8e-041
gb|BU975384.1|BU975384 HB31F04r BC Hordeum vulgare subsp. v... 152 4e-040
gb|AJ462109.1|AJ462109 AJ462109 S00002 Hordeum vulgare subs... 150 1e-035
gb|CA013755.1|CA013755 HT09G07r HT Hordeum vulgare subsp. v... 136 2e-034
gb|BJ459872.1|BJ459872 BJ459872 K. Sato unpublished cDNA li... 89 4e-028
gb|AV911022.1|AV911022 AV911022 K. Sato unpublished cDNA li... 89 4e-028
gb|BM816328.1|BM816328 HC108D12_SK.ab1 HC Hordeum vulgare s... 122 3e-027
gb|BJ452323.1|BJ452323 BJ452323 K. Sato unpublished cDNA li... 80 1e-025
gb|AV909139.1|AV909139 AV909139 K. Sato unpublished cDNA li... 79 4e-025
gb|AV928242.1|AV928242 AV928242 K. Sato unpublished cDNA li... 68 2e-021
gb|BJ467826.1|BJ467826 BJ467826 K. Sato unpublished cDNA li... 68 2e-021
gb|BG368552.1|BG368552 HVSMEi0019L05f Hordeum vulgare 20 DA... 68 2e-021
gb|CA001439.1|CA001439 HS17O15u HS Hordeum vulgare subsp. v... 102 3e-021
gb|CA010057.1|CA010057 HT11K20u HT Hordeum vulgare subsp. v... 102 3e-021
>gb|BE558753.3|BE558753 HV_CEb0020E08f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0020E08f, mRNA sequence
Length = 790
Score = 240 bits (519), Expect(4) = e-111
Identities = 100/132 (75%), Positives = 110/132 (83%)
Frame = -3 / +1
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HV+DLAEGH+ ALRKLF+SSSNIGCE YNLGTGKGTSVLEIVNAFEKASGKK
Sbjct: 199 GTGVRDYIHVLDLAEGHIAALRKLFDSSSNIGCEPYNLGTGKGTSVLEIVNAFEKASGKK 378
Query: 627 IPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSPYGYAASESPK 448
IPLV+GQRRPGDAEILF+ KAEREL+WKAKYGITEMC D WNWASK+PYGY + +S K
Sbjct: 379 IPLVVGQRRPGDAEILFAGTGKAERELNWKAKYGITEMCRDQWNWASKNPYGYGSPDSTK 558
Query: 447 LNGSSR*ATPIG 412
NG+S * G
Sbjct: 559 QNGNSH*TEQRG 594
Score = 206 bits (445), Expect(4) = 2e-080
Identities = 95/129 (73%), Positives = 98/129 (75%)
Frame = +2 / -3
Query: 428 QRELPFSFGDSDAAYP*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRR 607
Q ELPF +S YP*G LLAQFH S HISVMPYFAFQ SSLSA KRISASPGRR
Sbjct: 578 QCELPFCLVESGDPYP*GFLLAQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR 399
Query: 608 WPMTKGIFFPEAFSNALTISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCPSARSTT*D 787
P T GIFFPEAFSNA TISNTDVPFPVPRLY SHPMLDELSKSF +A CPSARS+T*
Sbjct: 398 *PTTSGIFFPEAFSNAFTISNTDVPFPVPRLYGSHPMLDELSKSFRSAAICPSARSST*I 219
Query: 788 IITNPCPIL 814
P P L
Sbjct: 218 *SRTPVPSL 192
Score = 161 bits (346), Expect(4) = e-111
Identities = 63/69 (91%), Positives = 65/69 (94%)
Frame = -2 / +1
Query: 991 SDPEWNIILLRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATK 812
SD EW IILLRYFNPVGAHPSGYLGEDP G PNNLMPFVQQ+AVGRRPS+TIFGNNYATK
Sbjct: 16 SDSEWRIILLRYFNPVGAHPSGYLGEDPRGVPNNLMPFVQQVAVGRRPSVTIFGNNYATK 195
Query: 811 DGTGVRDYI 785
DGTGVRDYI
Sbjct: 196 DGTGVRDYI 222
Score = 155 bits (332), Expect(3) = 8e-074
Identities = 72/114 (63%), Positives = 78/114 (68%)
Frame = +1 / -2
Query: 442 IQLRRLRCRVSIRALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQ 621
+ L R+ V +R LASPVP V AH DAI FPVE SLCF KENL +TR L DQ
Sbjct: 564 VLLGRVG*PVPVRVLASPVPLVPAHLRDAILCFPVEFSLCFTSSCKENLRVTRAALTDDQ 385
Query: 622 RDFLSRSLLKCIDYLQH*CSFSGSKVVCFTSYVR*AFKKLP*CEKMSFSKVNHM 783
RDFLSRSLL+CI YLQH* FS SKVV FTSYVR KKLP*C M SK+ HM
Sbjct: 384 RDFLSRSLLECIYYLQH*RPFSSSKVVWFTSYVRRTIKKLP*CSNMPLSKIKHM 223
Score = 144 bits (310), Expect(3) = 1e-064
Identities = 67/110 (60%), Positives = 75/110 (68%)
Frame = -1 / +2
Query: 791 LYLMWLTLLKDIFSH*GSFLKAHLT*DVKHTTLEPEKEHQCWR*SMHLRRLLERKSLWSW 612
+ M L LL+ I H GSFL LT*DV HTTLE EK QCWR* MH RRLLERKS WS
Sbjct: 215 IIFMCLILLRGILLHYGSFLIVRLT*DVNHTTLELEKGRQCWR**MHSRRLLERKSRWSS 394
Query: 611 ANGVLVMLRFSFHRRQKQRESSTGKRNMASQKCAETCGTGLARALMDTRH 462
+ LV RFS KQRE+STGK++MAS++CA T GTGLAR L T H
Sbjct: 395 VSAALVTRRFSLQELVKQRENSTGKQSMASRRCAGTSGTGLARTLTGTGH 544
Score = 143 bits (306), Expect = 2e-033
Identities = 68/120 (56%), Positives = 81/120 (67%)
Frame = -2 / +3
Query: 784 SCG*PC*RTSFRIKEAF*KLI*HRM*SIQPWNRKRNISVGDSQCI*EGFWKENPFGHGPT 605
SC * C* EAF* +*HRM*+IQPWN KR++SVGDS+CI EGFWKENP G
Sbjct: 222 SCA*SC*GAYCCTTEAF**FV*HRM*TIQPWNWKRDVSVGDSKCIREGFWKENPAGRRSA 401
Query: 604 ASW*C*DSLFIAGKSRERAPLESEIWHHRNVRRPVELG*QEPLWIRGI*VSEAEW*FPLS 425
W* DSL SRER LES++WHH +V+ PVELG*QEPL +R + +AE F L+
Sbjct: 402 PPW*RGDSLCRNW*SRERTQLESKVWHHGDVQGPVELG*QEPLRVRVTRLDQAERQFTLN 581
Score = 135 bits (290), Expect(3) = 8e-074
Identities = 59/69 (85%), Positives = 61/69 (88%)
Frame = +3 / -3
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSPRYPLG*APTGLKYLS 965
I*SRTPVPS VA*LFP IV+DGLLPTA C*TKGIRL G P GSSPRYPLG*APTGLKYLS
Sbjct: 221 I*SRTPVPSLVA*LFPKIVTDGLLPTATC*TKGIRLFGTPRGSSPRYPLG*APTGLKYLS 42
Query: 966 KMIFHSGSE 992
KMI HS S+
Sbjct: 41 KMILHSESD 15
Score = 130 bits (279), Expect(3) = 1e-057
Identities = 61/119 (51%), Positives = 75/119 (63%)
Frame = +3 / -1
Query: 429 SGNYHSASETQMPRIHKGSC*PSSTGLRTFL*CHIXXXXXXXXXXXPAMKRESQHHQDAV 608
S N SA +++ R KGSC*PSSTG T *CH ++RES HQ
Sbjct: 577 SVNCRSAWSSRVTRTRKGSC*PSSTGPCTSP*CHTLLSS*VLSLLYQFLQRESPRHQGGA 398
Query: 609 GP*PKGFSFQKPSQMH*LSPTLMFLFRFQGCMLHILC*MSFQKASLMRKDVLQQGQPHE 785
P GFSFQKPS+MH LSPTL LF+FQGCM+HILC* ++QKAS++++ QQ Q HE
Sbjct: 397 DRRPAGFSFQKPSRMHLLSPTLTSLFQFQGCMVHILC*TNYQKASVVQQYAPQQDQAHE 221
Score = 113 bits (241), Expect(3) = 1e-064
Identities = 44/68 (64%), Positives = 53/68 (77%)
Frame = -1 / +3
Query: 989 RS*MEYHFA*VL*SSWGSPQWIPW*RPMWNS*QPYALRSANCSWQEAIANNIWK*LRNKG 810
R *+E HF *V WGSPQWIPW*RP W+S QPYALRSA+C W+EA+++N+WK*L N
Sbjct: 18 RF*VEDHFT*VFQPGWGSPQWIPW*RPTWSSKQPYALRSASCCWEEAVSDNLWK*LCN*R 197
Query: 809 WDRGS*LY 786
WD G+ LY
Sbjct: 198 WDWGTRLY 221
Score = 110 bits (234), Expect(3) = 1e-057
Identities = 47/69 (68%), Positives = 54/69 (78%)
Frame = +2 / -2
Query: 785 DIITNPCPILCCVVISKYC*RWPPANCNLLNEGHKVVRSSTWVFTKVSTGVSPNWIKVPK 964
+II+ P PI C+VISK C PP N NLLNEGHKVV +STWVFTKVSTGVSPN +++PK
Sbjct: 222 NIISYPSPIFSCIVISKDCH*RPPPNSNLLNEGHKVVWNSTWVFTKVSTGVSPNRVEIPK 43
Query: 965 QNDIPFRI* 991
ND P RI*
Sbjct: 42 *NDPPLRI* 16
Score = 106 bits (226), Expect = 2e-022
Identities = 51/79 (64%), Positives = 57/79 (72%)
Frame = -3 / +2
Query: 990 QILNGISFCLGTLIQLGLTPVDTLVKTHVELLTTLCPSFSKLQLAGGHR*QYLEITTQQR 811
QIL+G SF LG +LGLTPVDTLVKTHVE TTLCPSFSKL L GG +*Q LEIT Q +
Sbjct: 17 QILSGGSFYLGISTRLGLTPVDTLVKTHVEFQTTLCPSFSKLLLGGGRQ*QSLEITMQLK 196
Query: 810 MGQGFVIISHVVDLAEGHL 754
MG G+ II + L G L
Sbjct: 197 MGLGYEIIFMCLILLRGIL 253
Score = 95.0 bits (201), Expect(4) = 2e-080
Identities = 45/68 (66%), Positives = 48/68 (70%)
Frame = +1 / -1
Query: 787 YNHEPLSHPLLRSYFQILLAMASCQLQFAERRA*GC*EFHMGLHQGIHWGEPQLD*ST*A 966
YN P SH L SYFQ L AS Q Q AERRA*GC E H+GLHQGIHWGEPQ *+T*
Sbjct: 220 YNLVPQSHL*LHSYFQRLSLTASSQQQLAERRA*GCLELHVGLHQGIHWGEPQPG*NT*V 41
Query: 967 K*YSIQDL 990
K* S Q+L
Sbjct: 40 K*SSTQNL 17
Score = 29.5 bits (58), Expect(4) = e-111
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -1 / +3
Query: 383 VVPTNQDKCRSSRSVI 336
+VPT+ D+CRSSR VI
Sbjct: 627 LVPTSWDRCRSSRPVI 674
Score = 29.5 bits (58), Expect(4) = e-111
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / +2
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 581 LNNADRSVWTHLYMQL 628
Score = 28.1 bits (55), Expect(4) = 2e-080
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2 / -1
Query: 389 HVHASPHGPIGVAQ 430
HV SPHGPI V Q
Sbjct: 622 HVQVSPHGPIRVVQ 581
Score = 28.1 bits (55), Expect(4) = 2e-080
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1 / -1
Query: 337 MTDRLDLHLSWLVGT 381
MT RLDLH S LVGT
Sbjct: 673 MTGRLDLHRSQLVGT 629
Score = 27.6 bits (54), Expect(3) = 1e-064
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / +3
Query: 429 *ATPIGPCGLACT 391
* T IGPCGL CT
Sbjct: 582 *TTRIGPCGLTCT 620
Score = 26.3 bits (51), Expect(3) = 8e-074
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +1 / -3
Query: 385 VSCTCESTRPNRRCS 429
V+CT ESTR + RCS
Sbjct: 626 VACTGESTRTDPRCS 582
Score = 22.1 bits (42), Expect(3) = 1e-057
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +3 / -2
Query: 384 SLMYMRVHTAQSALLS 431
S MY VHT +SAL S
Sbjct: 627 SCMYR*VHTDRSALFS 580
>gb|BJ463662.1|BJ463662 BJ463662 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32p11 5', mRNA sequence
Length = 540
Score = 211 bits (455), Expect(2) = 3e-092
Identities = 87/106 (82%), Positives = 93/106 (87%)
Frame = -3 / +1
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HV+DLAEGH+ ALRKLF+SSSNIGCE YNLGTGKGTSVLEIVNAFEKASGKK
Sbjct: 223 GTGVRDYIHVLDLAEGHIAALRKLFDSSSNIGCEPYNLGTGKGTSVLEIVNAFEKASGKK 402
Query: 627 IPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWA 490
IPLV+GQRRPGDAEILF+ KAEREL+WKAKYGITEMC D WNWA
Sbjct: 403 IPLVVGQRRPGDAEILFAGTGKAERELNWKAKYGITEMCRDQWNWA 540
Score = 183 bits (394), Expect = 1e-045
Identities = 83/108 (76%), Positives = 85/108 (78%)
Frame = +2 / -2
Query: 491 AQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSNALTISN 670
AQFH S HISVMPYFAFQ SSLSA KRISASPGRR P T GIFFPEAFSNA TISN
Sbjct: 539 AQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR*PTTSGIFFPEAFSNAFTISN 360
Query: 671 TDVPFPVPRLYASHPMLDELSKSFLNAKRCPSARSTT*DIITNPCPIL 814
TDVPFPVPRLY SHPMLDELSKSF +A CPSARS+T* P P L
Sbjct: 359 TDVPFPVPRLYGSHPMLDELSKSFRSAAICPSARSST*I*SRTPVPSL 216
Score = 148 bits (317), Expect(2) = 3e-092
Identities = 58/62 (93%), Positives = 60/62 (96%)
Frame = -2 / +1
Query: 970 ILLRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATKDGTGVRD 791
ILLRYFNPVGAHPSGYLGEDP G PNNLMPFVQQ+AVGRRPS+TIFGNNYATKDGTGVRD
Sbjct: 61 ILLRYFNPVGAHPSGYLGEDPRGVPNNLMPFVQQVAVGRRPSVTIFGNNYATKDGTGVRD 240
Query: 790 YI 785
YI
Sbjct: 241 YI 246
Score = 145 bits (312), Expect(2) = 2e-066
Identities = 66/98 (67%), Positives = 69/98 (70%)
Frame = +1 / -1
Query: 490 SPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQRDFLSRSLLKCIDYLQ 669
SPVP V AH DAI FPVE SLCF KENL +TR L DQRDFLSRSLL+CI YLQ
Sbjct: 540 SPVPLVPAHLRDAILCFPVEFSLCFTSSCKENLRVTRAALTDDQRDFLSRSLLECIYYLQ 361
Query: 670 H*CSFSGSKVVCFTSYVR*AFKKLP*CEKMSFSKVNHM 783
H* FS SKVV FTSYVR KKLP*C M SK+ HM
Sbjct: 360 H*RPFSSSKVVWFTSYVRRTIKKLP*CSNMPLSKIKHM 247
Score = 133 bits (284), Expect(2) = 2e-056
Identities = 61/100 (61%), Positives = 69/100 (69%)
Frame = -1 / +2
Query: 791 LYLMWLTLLKDIFSH*GSFLKAHLT*DVKHTTLEPEKEHQCWR*SMHLRRLLERKSLWSW 612
+ M L LL+ I H GSFL LT*DV HTTLE EK QCWR* MH RRLLERKS WS
Sbjct: 239 IIFMCLILLRGILLHYGSFLIVRLT*DVNHTTLELEKGRQCWR**MHSRRLLERKSRWSS 418
Query: 611 ANGVLVMLRFSFHRRQKQRESSTGKRNMASQKCAETCGTG 492
+ LV RFS KQRE+STGK++MAS++CA T GTG
Sbjct: 419 VSAALVTRRFSLQELVKQRENSTGKQSMASRRCAGTSGTG 538
Score = 127 bits (272), Expect(2) = 2e-066
Identities = 57/74 (77%), Positives = 59/74 (79%)
Frame = +3 / -2
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSPRYPLG*APTGLKYLS 965
I*SRTPVPS VA*LFP IV+DGLLPTA C*TKGIRL G P GSSPRYPLG*APTGLKYLS
Sbjct: 245 I*SRTPVPSLVA*LFPKIVTDGLLPTATC*TKGIRLFGTPRGSSPRYPLG*APTGLKYLS 66
Query: 966 KMIFHSGSECRYHG 1007
K+ YHG
Sbjct: 65 KISSTQNLTGIYHG 24
Score = 127 bits (272), Expect = 1e-028
Identities = 58/98 (59%), Positives = 67/98 (68%)
Frame = -2 / +3
Query: 784 SCG*PC*RTSFRIKEAF*KLI*HRM*SIQPWNRKRNISVGDSQCI*EGFWKENPFGHGPT 605
SC * C* EAF* +*HRM*+IQPWN KR++SVGDS+CI EGFWKENP G
Sbjct: 246 SCA*SC*GAYCCTTEAF**FV*HRM*TIQPWNWKRDVSVGDSKCIREGFWKENPAGRRSA 425
Query: 604 ASW*C*DSLFIAGKSRERAPLESEIWHHRNVRRPVELG 491
W* DSL SRER LES++WHH +V+ PVELG
Sbjct: 426 PPW*RGDSLCRNW*SRERTQLESKVWHHGDVQGPVELG 539
Score = 111 bits (238), Expect(2) = 3e-048
Identities = 51/98 (52%), Positives = 62/98 (63%)
Frame = +3 / -3
Query: 492 PSSTGLRTFL*CHIXXXXXXXXXXXPAMKRESQHHQDAVGP*PKGFSFQKPSQMH*LSPT 671
PSSTG T *CH ++RES HQ P GFSFQKPS+MH LSPT
Sbjct: 538 PSSTGPCTSP*CHTLLSS*VLSLLYQFLQRESPRHQGGADRRPAGFSFQKPSRMHLLSPT 359
Query: 672 LMFLFRFQGCMLHILC*MSFQKASLMRKDVLQQGQPHE 785
L LF+FQGCM+HILC* ++QKAS++++ QQ Q HE
Sbjct: 358 LTSLFQFQGCMVHILC*TNYQKASVVQQYAPQQDQAHE 245
Score = 107 bits (228), Expect(2) = 2e-056
Identities = 40/62 (64%), Positives = 49/62 (79%)
Frame = -1 / +3
Query: 971 HFA*VL*SSWGSPQWIPW*RPMWNS*QPYALRSANCSWQEAIANNIWK*LRNKGWDRGS* 792
+F *V WGSPQWIPW*RP W+S QPYALRSA+C W+EA+++N+WK*L N WD G+
Sbjct: 60 YFT*VFQPGWGSPQWIPW*RPTWSSKQPYALRSASCCWEEAVSDNLWK*LCN*RWDWGTR 239
Query: 791 LY 786
LY
Sbjct: 240 LY 245
Score = 100 bits (214), Expect(2) = 3e-048
Identities = 42/62 (67%), Positives = 49/62 (79%)
Frame = +2 / -1
Query: 785 DIITNPCPILCCVVISKYC*RWPPANCNLLNEGHKVVRSSTWVFTKVSTGVSPNWIKVPK 964
+II+ P PI C+VISK C PP N NLLNEGHKVV +STWVFTKVSTGVSPN +++PK
Sbjct: 246 NIISYPSPIFSCIVISKDCH*RPPPNSNLLNEGHKVVWNSTWVFTKVSTGVSPNRVEIPK 67
Query: 965 QN 970
N
Sbjct: 66 *N 61
>gb|CA013313.1|CA013313 HT08A11r HT Hordeum vulgare subsp. vulgare cDNA clone HT08A11
5-PRIME, mRNA sequence
Length = 646
Score = 200 bits (432), Expect(2) = 1e-081
Identities = 80/118 (67%), Positives = 95/118 (80%)
Frame = -3 / +3
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HVVDLA+GH+ ALRKL+E S IGCE YNLGTGKGTSVLE+V AFEKA GKK
Sbjct: 201 GTGVRDYIHVVDLADGHIAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKK 380
Query: 627 IPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSPYGYAASES 454
IPLV RR GDAE ++++ AKAE+EL WKAK+G+ EMC DLWNWASK+PYGY +S++
Sbjct: 381 IPLVYAGRRAGDAETVYAATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSSDN 554
Score = 144 bits (310), Expect(2) = 2e-041
Identities = 71/114 (62%), Positives = 76/114 (66%)
Frame = +2 / -1
Query: 473 P*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSN 652
P*G LLAQFHRS HIS P AF SS SA A +SASP T+GIFFP+AFSN
Sbjct: 535 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 356
Query: 653 ALTISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCPSARSTT*DIITNPCPIL 814
A TIS+TDVPFPVPRLY SHP+L E S SFL A PSARSTT P P L
Sbjct: 355 AATISSTDVPFPVPRLYTSHPILSESS*SFLKAAMWPSARSTTWM*SRTPVPSL 194
Score = 123 bits (263), Expect(2) = 1e-081
Identities = 45/56 (80%), Positives = 52/56 (92%)
Frame = -2 / +3
Query: 952 NPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATKDGTGVRDYI 785
NPVGAHPSGY+GEDP G PNNLMP+VQQ+AVGRRP+LT++G +Y TKDGTGVRDYI
Sbjct: 57 NPVGAHPSGYIGEDPLGVPNNLMPYVQQVAVGRRPALTVYGTDYNTKDGTGVRDYI 224
Score = 84.5 bits (178), Expect(2) = 2e-034
Identities = 44/105 (41%), Positives = 63/105 (60%)
Frame = +1 / -3
Query: 469 VSIRALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQRDFLSRSLL 648
V++ LA PVPQVSAH P+EL LCF +L I+ + + +QRDFLS SLL
Sbjct: 539 VAVGVLACPVPQVSAHLLHPKLGLPLELLLCFGSGSVHSLGISSSSSSINQRDFLSASLL 360
Query: 649 KCIDYLQH*CSFSGSKVVCFTSYVR*AFKKLP*CEKMSFSKVNHM 783
+C ++LQH S S +++V F Y F +LP ++ S+VN+M
Sbjct: 359 ECGNHLQHGRSLSSAQIVHFAPYSVRIFVELPQGCDVAVSQVNNM 225
Score = 82.2 bits (173), Expect(2) = 1e-024
Identities = 45/100 (45%), Positives = 52/100 (52%)
Frame = -1 / +1
Query: 782 MWLTLLKDIFSH*GSFLKAHLT*DVKHTTLEPEKEHQCWR*SMHLRRLLERKSLWSWANG 603
M LT L *GS K * K T E+E CWR H RRL ERKSLW
Sbjct: 226 MLLTWLTATSQP*GSSTKILTE*GAKCTIWALERERPCWRWLPHSRRLAERKSLWFMLEE 405
Query: 602 VLVMLRFSFHRRQKQRESSTGKRNMASQKCAETCGTGLAR 483
L M R KQR SS+G+ ++ ++CAETCGTG AR
Sbjct: 406 ELEMPRLCTLPLPKQRRSSSGRPSLGWRRCAETCGTGQAR 525
Score = 81.7 bits (172), Expect(2) = 2e-034
Identities = 40/56 (71%), Positives = 41/56 (73%)
Frame = +3 / -1
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSPRYPLG*APTGL 953
+*SRTPVPS V * P VS G LPTA C T GIRLLG P GSSP PLG*APTGL
Sbjct: 223 M*SRTPVPSLVL*SVP*TVSAGRLPTATCWT*GIRLLGTPRGSSPM*PLG*APTGL 56
Score = 79.0 bits (166), Expect(2) = 4e-023
Identities = 44/105 (41%), Positives = 60/105 (57%)
Frame = -2 / +2
Query: 781 CG*PC*RTSFRIKEAF*KLI*HRM*SIQPWNRKRNISVGDSQCI*EGFWKENPFGHGPTA 602
C *P *R ++EA + +R+ S+Q + K N+ GD I EG KENP G
Sbjct: 227 CC*PG*RPHRSLEEALRRF*QNRVRSVQSGHWKGNVRAGDGCRIREGLRKENPSGLCWKK 406
Query: 601 SW*C*DSLFIAGKSRERAPLESEIWHHRNVRRPVELG*QEPLWIR 467
SW C D + +SRE A +E ++W +V+RPVELG QEPL +R
Sbjct: 407 SWRCRDCVRCHCQSREGAQVEGQVWGGGDVQRPVELGKQEPLRLR 541
Score = 51.0 bits (105), Expect(2) = 1e-024
Identities = 22/54 (40%), Positives = 35/54 (64%)
Frame = -1 / +2
Query: 947 SWGSPQWIPW*RPMWNS*QPYALRSANCSWQEAIANNIWK*LRNKGWDRGS*LY 786
SW SP+W+ RP W+ QP AL A+ + A A+ + L+++GW+RG+*L+
Sbjct: 62 SWRSPEWLHRGRPPWSPQQPDALCPASRCREAACADGLRDRLQHQGWNRGT*LH 223
Score = 49.2 bits (101), Expect(2) = 4e-023
Identities = 28/57 (49%), Positives = 32/57 (56%)
Frame = -3 / +1
Query: 954 LIQLGLTPVDTLVKTHVELLTTLCPSFSKLQLAGGHR*QYLEITTQQRMGQGFVIIS 784
L QL LT V T KT +E TT CP SK GG R*++ TT RM QG+V S
Sbjct: 55 LTQLALTRVATSGKTPLESPTT*CPMSSKSLSGGGLR*RFTGPTTTPRMEQGYVTTS 225
Score = 45.1 bits (92), Expect(2) = 2e-041
Identities = 19/37 (51%), Positives = 25/37 (67%)
Frame = +2 / -3
Query: 845 RWPPANCNLLNEGHKVVRSSTWVFTKVSTGVSPNWIK 955
R PP + +LL+ GH+VV S VF V+T VS NW+K
Sbjct: 164 RRPPPDSDLLDIGHQVVGDSKGVFPDVATRVSANWVK 54
>gb|BE420919.1|BE420919 HWM003.H08 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM003.H08, mRNA sequence
Length = 1212
Score = 177 bits (382), Expect(3) = 5e-081
Identities = 74/110 (67%), Positives = 86/110 (78%)
Frame = -3 / +2
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HVVDLA+GH+ ALRKL+E S IGCE YNLGTGKGTSVLE+V AFEKA GKK
Sbjct: 314 GTGVRDYIHVVDLADGHIAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKK 493
Query: 627 IPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSP 478
IPLV RR GDAE ++++ AKAE+EL WKAK+ + EMC DLWN ASK+P
Sbjct: 494 IPLVYAGRRAGDAETVYAATAKAEKELKWKAKFRLEEMCRDLWNLASKNP 643
Score = 135 bits (289), Expect(3) = 4e-044
Identities = 67/112 (59%), Positives = 73/112 (65%)
Frame = +2 / -1
Query: 479 GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSNAL 658
G LLA+FHRS HIS AF SS SA A +SASP T+GIFFP+AFSNA
Sbjct: 642 GFLLAKFHRSLHISSSRNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSNAA 463
Query: 659 TISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCPSARSTT*DIITNPCPIL 814
TIS+TDVPFPVPRLY SHP+L E S SFL A PSARSTT P P L
Sbjct: 462 TISSTDVPFPVPRLYTSHPILSESS*SFLKAAMWPSARSTTWM*SRTPVPSL 307
Score = 90.0 bits (190), Expect(3) = 5e-081
Identities = 33/45 (73%), Positives = 40/45 (88%)
Frame = -2 / +2
Query: 919 GEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATKDGTGVRDYI 785
G+ P G PNNLMP+VQQ+AVGRRP+LT++G +Y TKDGTGVRDYI
Sbjct: 203 GKTPFGVPNNLMPYVQQVAVGRRPALTVYGTHYNTKDGTGVRDYI 337
Score = 80.8 bits (170), Expect(2) = 3e-026
Identities = 43/102 (42%), Positives = 60/102 (58%)
Frame = +1 / -3
Query: 478 RALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQRDFLSRSLLKCI 657
R LA VPQVSAH P+EL LCF +L I+ + + +QRDFLS SLL+C
Sbjct: 643 RVLACQVPQVSAHLLQPKLGLPLELLLCFGSGSVHSLGISSSSSSINQRDFLSASLLECG 464
Query: 658 DYLQH*CSFSGSKVVCFTSYVR*AFKKLP*CEKMSFSKVNHM 783
++LQH S S +++V F Y F +LP ++ S+VN+M
Sbjct: 463 NHLQHGRSLSSAQIVHFAPYSVRIFVELPQGCDVAVSQVNNM 338
Score = 80.3 bits (169), Expect(3) = 1e-022
Identities = 45/103 (43%), Positives = 52/103 (50%)
Frame = -1 / +3
Query: 782 MWLTLLKDIFSH*GSFLKAHLT*DVKHTTLEPEKEHQCWR*SMHLRRLLERKSLWSWANG 603
M LT L *GS K * K T E+E CWR H RRL ERKSLW
Sbjct: 339 MLLTWLTATSQP*GSSTKILTE*GAKCTIWALERERPCWRWLPHSRRLAERKSLWFMLEE 518
Query: 602 VLVMLRFSFHRRQKQRESSTGKRNMASQKCAETCGTGLARALM 474
L M R KQR SS+G+ + ++CAETCGT AR L+
Sbjct: 519 ELEMPRLCTLPLPKQRRSSSGRPSFGWRRCAETCGTWQARTLL 647
Score = 73.5 bits (154), Expect(3) = 5e-081
Identities = 26/32 (81%), Positives = 29/32 (90%)
Frame = -2 / +1
Query: 991 SDPEWNIILLRYFNPVGAHPSGYLGEDPCGTP 896
SDP+W IILLRYFNPVGAHPSGY+GEDP +P
Sbjct: 130 SDPDWKIILLRYFNPVGAHPSGYIGEDPLRSP 225
Score = 57.9 bits (120), Expect(2) = 3e-026
Identities = 29/45 (64%), Positives = 30/45 (66%)
Frame = +3 / -1
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSP 920
+*SRTPVPS V * P VS G LPTA C T GIRLLG P G P
Sbjct: 336 M*SRTPVPSLVL*WVP*TVSAGRLPTATCWT*GIRLLGTPKGVFP 202
Score = 57.4 bits (119), Expect(3) = 4e-044
Identities = 25/32 (78%), Positives = 26/32 (81%)
Frame = +3 / -2
Query: 897 GVPHGSSPRYPLG*APTGLKYLSKMIFHSGSE 992
G+ GSSP PLG*APTGLKYLS MIF SGSE
Sbjct: 224 GLRRGSSPM*PLG*APTGLKYLSSMIFQSGSE 129
Score = 35.4 bits (71), Expect(3) = 1e-022
Identities = 18/37 (48%), Positives = 21/37 (56%)
Frame = -3 / +3
Query: 894 TTLCPSFSKLQLAGGHR*QYLEITTQQRMGQGFVIIS 784
TT CP SK GG R*++ TT RM QG+V S
Sbjct: 228 TT*CPMSSKSLSGGGLR*RFTGPTTTPRMEQGYVTTS 338
Score = 28.1 bits (55), Expect(3) = 1e-022
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -1 / +3
Query: 980 MEYHFA*VL*SSWGSPQWIPW*RP 909
+E H A VL SW SP+W+ RP
Sbjct: 141 LEDHTAEVLQPSWRSPEWLHRGRP 212
Score = 24.4 bits (47), Expect(3) = 4e-044
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +1 / -2
Query: 790 NHEPLSHPLLRSYFQILLAMASCQLQFAERRA*GC 894
+H PL HP S + A A+ + + A RA GC
Sbjct: 332 SHVPLFHPWCCSGSRKPSAQAASRQRLAGHRASGC 228
>gb|CA013080.1|CA013080 HT07F09r HT Hordeum vulgare subsp. vulgare cDNA clone HT07F09
5-PRIME, mRNA sequence
Length = 637
Score = 153 bits (328), Expect(3) = 1e-075
Identities = 59/87 (67%), Positives = 74/87 (85%)
Frame = -3 / +1
Query: 729 SSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERE 550
++ +IGC AYNLGTG+GT+VLE+V+AFEKA GKKIP+ M RRPGD+E +++S AKAE+E
Sbjct: 286 ATPDIGCVAYNLGTGRGTTVLEMVSAFEKAYGKKIPVKMCPRRPGDSEQVYASTAKAEQE 465
Query: 549 LHWKAKYGITEMCGDLWNWASKSPYGY 469
L W+AKYGI EMC D WNWA K+PYGY
Sbjct: 466 LGWRAKYGIEEMCRDQWNWARKNPYGY 546
Score = 135 bits (289), Expect(3) = 1e-075
Identities = 49/78 (62%), Positives = 63/78 (80%)
Frame = -2 / +1
Query: 1018 RPRRP*YLHSDPEWNIILLRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLT 839
R R + H++PEW ILLRYFNP+GAH SG +GEDP G PNNL+P++QQ+AVGRRP L
Sbjct: 1 RTRAARHPHANPEWRTILLRYFNPIGAHQSGDIGEDPKGVPNNLLPYIQQVAVGRRPELN 180
Query: 838 IFGNNYATKDGTGVRDYI 785
++G++Y T+DGT VRDYI
Sbjct: 181 VYGHDYRTRDGTAVRDYI 234
Score = 103 bits (219), Expect(3) = 3e-039
Identities = 50/82 (60%), Positives = 54/82 (65%)
Frame = +2 / -3
Query: 473 P*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSN 652
P G LAQFH S HIS +PY A Q SS SA A D S SPG + GIF P AFSN
Sbjct: 542 PYGFFLAQFHWSLHISSIPYLALQPSSCSALAVDAYTCSESPGLLGHIFTGIFLPYAFSN 363
Query: 653 ALTISNTDVPFPVPRLYASHPM 718
ALTIS+T VP PVPRLYA+ PM
Sbjct: 362 ALTISSTVVPRPVPRLYATQPM 297
Score = 71.6 bits (150), Expect(3) = 3e-039
Identities = 37/70 (52%), Positives = 43/70 (61%)
Frame = +3 / -3
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSPRYPLG*APTGLKYLS 965
+*S T VPS V * +P +S G PTA C G RLLG P GSSP PL AP GLK S
Sbjct: 233 M*SLTAVPSRVR*SWP*TLSSGRRPTATCWM*GSRLLGTPLGSSPMSPLWWAPMGLK*RS 54
Query: 966 KMIFHSGSEC 995
+++ HSG C
Sbjct: 53 RIVLHSGFAC 24
Score = 34.5 bits (69), Expect(3) = 1e-075
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = -3 / +1
Query: 783 HVVDLAEGHLFALRKLF 733
HVVDLA+GH+ AL KLF
Sbjct: 235 HVVDLADGHIAALEKLF 285
Score = 25.8 bits (50), Expect(3) = 3e-039
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2 / -3
Query: 728 LSKSFLNAKRCPSARSTT 781
++KSF +A PSARSTT
Sbjct: 290 VAKSFSSAAMWPSARSTT 237
>gb|BU990717.1|BU990717 HF25N16r HF Hordeum vulgare subsp. vulgare cDNA clone HF25N16
5-PRIME, mRNA sequence
Length = 633
Score = 200 bits (432), Expect(2) = 6e-071
Identities = 80/118 (67%), Positives = 95/118 (80%)
Frame = -3 / +3
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HVVDLA+GH+ ALRKL+E S IGCE YNLGTGKGTSVLE+V AFEKA GKK
Sbjct: 114 GTGVRDYIHVVDLADGHIAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKK 293
Query: 627 IPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSPYGYAASES 454
IPLV RR GDAE ++++ AKAE+EL WKAK+G+ EMC DLWNWASK+PYGY +S++
Sbjct: 294 IPLVYAGRRAGDAETVYAATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSSDN 467
Score = 144 bits (310), Expect(2) = 1e-035
Identities = 71/114 (62%), Positives = 76/114 (66%)
Frame = +2 / -3
Query: 473 P*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSN 652
P*G LLAQFHRS HIS P AF SS SA A +SASP T+GIFFP+AFSN
Sbjct: 448 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 269
Query: 653 ALTISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCPSARSTT*DIITNPCPIL 814
A TIS+TDVPFPVPRLY SHP+L E S SFL A PSARSTT P P L
Sbjct: 268 AATISSTDVPFPVPRLYTSHPILSESS*SFLKAAMWPSARSTTWM*SRTPVPSL 107
Score = 87.7 bits (185), Expect(2) = 6e-071
Identities = 32/42 (76%), Positives = 38/42 (90%)
Frame = -2 / +3
Query: 910 PCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATKDGTGVRDYI 785
P G PNNLMP+VQQ+AVGRRP+LT++G +Y TKDGTGVRDYI
Sbjct: 12 PLGVPNNLMPYVQQVAVGRRPALTVYGTDYNTKDGTGVRDYI 137
Score = 84.5 bits (178), Expect(2) = 5e-027
Identities = 44/105 (41%), Positives = 63/105 (60%)
Frame = +1 / -2
Query: 469 VSIRALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQRDFLSRSLL 648
V++ LA PVPQVSAH P+EL LCF +L I+ + + +QRDFLS SLL
Sbjct: 452 VAVGVLACPVPQVSAHLLHPKLGLPLELLLCFGSGSVHSLGISSSSSSINQRDFLSASLL 273
Query: 649 KCIDYLQH*CSFSGSKVVCFTSYVR*AFKKLP*CEKMSFSKVNHM 783
+C ++LQH S S +++V F Y F +LP ++ S+VN+M
Sbjct: 272 ECGNHLQHGRSLSSAQIVHFAPYSVRIFVELPQGCDVAVSQVNNM 138
Score = 57.0 bits (118), Expect(2) = 5e-027
Identities = 28/42 (66%), Positives = 29/42 (69%)
Frame = +3 / -3
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHG 911
+*SRTPVPS V * P VS G LPTA C T GIRLLG P G
Sbjct: 136 M*SRTPVPSLVL*SVP*TVSAGRLPTATCWT*GIRLLGTPRG 11
Score = 25.4 bits (49), Expect(2) = 1e-035
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = +1 / -1
Query: 790 NHEPLSHPLLRSYFQILLAMASCQLQFAERRA*GC*EFHMGL 915
+H PL HP S + A A+ + + A RA GC GL
Sbjct: 132 SHVPLFHPWCCSRSRKPSAQAASRQRLAGHRASGCWGLQGGL 7
>gb|BU971800.1|BU971800 HB19L11r BC Hordeum vulgare subsp. vulgare cDNA clone HB19L11
5-PRIME, mRNA sequence
Length = 569
Score = 152 bits (327), Expect(2) = 4e-063
Identities = 56/69 (81%), Positives = 64/69 (92%)
Frame = -2 / +1
Query: 991 SDPEWNIILLRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATK 812
SDP+W IILLRYFNPVGAHPSGY+GEDP G PNNLMP+VQQ+AVGRRP+LT++G +Y TK
Sbjct: 187 SDPDWKIILLRYFNPVGAHPSGYIGEDPLGVPNNLMPYVQQVAVGRRPALTVYGTDYNTK 366
Query: 811 DGTGVRDYI 785
DGTGVRDYI
Sbjct: 367 DGTGVRDYI 393
Score = 109 bits (233), Expect(2) = 4e-063
Identities = 47/64 (73%), Positives = 51/64 (79%)
Frame = -3 / +1
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HVVDLA+GH+ ALRKL+E S IGCE YNLGTGKGTSVLE+V AFEKA GKK
Sbjct: 370 GTGVRDYIHVVDLADGHIAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKK 549
Query: 627 IPLV 616
IPLV
Sbjct: 550 IPLV 561
Score = 106 bits (226), Expect(2) = 2e-031
Identities = 51/69 (73%), Positives = 52/69 (75%)
Frame = +3 / -1
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSPRYPLG*APTGLKYLS 965
+*SRTPVPS V * P VS G LPTA C T GIRLLG P GSSP PLG*APTGLKYLS
Sbjct: 392 M*SRTPVPSLVL*SVP*TVSAGRLPTATCWT*GIRLLGTPRGSSPM*PLG*APTGLKYLS 213
Query: 966 KMIFHSGSE 992
MIF SGSE
Sbjct: 212 SMIFQSGSE 186
Score = 100 bits (213), Expect(2) = 9e-032
Identities = 45/66 (68%), Positives = 49/66 (74%)
Frame = +2 / -1
Query: 617 TKGIFFPEAFSNALTISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCPSARSTT*DIIT 796
T+GIFFP+AFSNA TIS+TDVPFPVPRLY SHP+L E S SFL A PSARSTT
Sbjct: 560 TRGIFFPQAFSNAATISSTDVPFPVPRLYTSHPILSESS*SFLKAAMWPSARSTTWM*SR 381
Query: 797 NPCPIL 814
P P L
Sbjct: 380 TPVPSL 363
Score = 57.0 bits (118), Expect(2) = 9e-032
Identities = 23/45 (51%), Positives = 32/45 (71%)
Frame = +2 / -3
Query: 845 RWPPANCNLLNEGHKVVRSSTWVFTKVSTGVSPNWIKVPKQNDIP 979
R PP + +LL+ GH+VV S VF V+T VS NW++VP+Q D+P
Sbjct: 333 RRPPPDSDLLDIGHQVVGDSKGVFPDVATRVSANWVEVPQQYDLP 199
Score = 50.1 bits (103), Expect(2) = 2e-031
Identities = 25/56 (44%), Positives = 37/56 (66%)
Frame = +1 / -3
Query: 616 DQRDFLSRSLLKCIDYLQH*CSFSGSKVVCFTSYVR*AFKKLP*CEKMSFSKVNHM 783
+QRDFLS SLL+C ++LQH S S +++V F Y F +LP ++ S+VN+M
Sbjct: 561 NQRDFLSASLLECGNHLQHGRSLSSAQIVHFAPYSVRIFVELPQGCDVAVSQVNNM 394
>gb|BU995681.1|BU995681 HM11A08r HM Hordeum vulgare subsp. vulgare cDNA clone HM11A08
5-PRIME, mRNA sequence
Length = 642
Score = 200 bits (432), Expect(2) = 8e-063
Identities = 80/118 (67%), Positives = 95/118 (80%)
Frame = -3 / +3
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HVVDLA+GH+ ALRKL+E S IGCE YNLGTGKGTSVLE+V AFEKA GKK
Sbjct: 108 GTGVRDYIHVVDLADGHIAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKK 287
Query: 627 IPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSPYGYAASES 454
IPLV RR GDAE ++++ AKAE+EL WKAK+G+ EMC DLWNWASK+PYGY +S++
Sbjct: 288 IPLVYAGRRAGDAETVYAATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSSDN 461
Score = 144 bits (310), Expect = 6e-034
Identities = 71/114 (62%), Positives = 76/114 (66%)
Frame = +2 / -3
Query: 473 P*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSN 652
P*G LLAQFHRS HIS P AF SS SA A +SASP T+GIFFP+AFSN
Sbjct: 442 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 263
Query: 653 ALTISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCPSARSTT*DIITNPCPIL 814
A TIS+TDVPFPVPRLY SHP+L E S SFL A PSARSTT P P L
Sbjct: 262 AATISSTDVPFPVPRLYTSHPILSESS*SFLKAAMWPSARSTTWM*SRTPVPSL 101
Score = 84.5 bits (178), Expect(2) = 1e-021
Identities = 44/105 (41%), Positives = 63/105 (60%)
Frame = +1 / -2
Query: 469 VSIRALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQRDFLSRSLL 648
V++ LA PVPQVSAH P+EL LCF +L I+ + + +QRDFLS SLL
Sbjct: 446 VAVGVLACPVPQVSAHLLHPKLGLPLELLLCFGSGSVHSLGISSSSSSINQRDFLSASLL 267
Query: 649 KCIDYLQH*CSFSGSKVVCFTSYVR*AFKKLP*CEKMSFSKVNHM 783
+C ++LQH S S +++V F Y F +LP ++ S+VN+M
Sbjct: 266 ECGNHLQHGRSLSSAQIVHFAPYSVRIFVELPQGCDVAVSQVNNM 132
Score = 60.6 bits (126), Expect(2) = 8e-063
Identities = 22/29 (75%), Positives = 27/29 (93%)
Frame = -2 / +3
Query: 871 QIAVGRRPSLTIFGNNYATKDGTGVRDYI 785
Q+AVGRRP+LT++G +Y TKDGTGVRDYI
Sbjct: 45 QVAVGRRPALTVYGTDYNTKDGTGVRDYI 131
Score = 39.1 bits (79), Expect(2) = 1e-021
Identities = 19/29 (65%), Positives = 20/29 (68%)
Frame = +3 / -3
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC 872
+*SRTPVPS V * P VS G LPTA C
Sbjct: 130 M*SRTPVPSLVL*SVP*TVSAGRLPTATC 44
>gb|BM371846.2|BM371846 EBma08_SQ004_C16_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ004_C16 5', mRNA sequence
Length = 398
Score = 133 bits (285), Expect(2) = 9e-062
Identities = 49/60 (81%), Positives = 56/60 (93%)
Frame = -2 / +1
Query: 964 LRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATKDGTGVRDYI 785
LRYFNPVGAHPSGY+GEDP G PNNLMP+VQQ+AVGRRP+LT++G +Y TKDGTGVRDYI
Sbjct: 1 LRYFNPVGAHPSGYIGEDPLGVPNNLMPYVQQVAVGRRPALTVYGTDYNTKDGTGVRDYI 180
Score = 124 bits (265), Expect(2) = 9e-062
Identities = 53/79 (67%), Positives = 61/79 (77%)
Frame = -3 / +1
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HVVDLA+GH+ ALRKL+E S IGCE YNLGTGKGTSVLE+V AFEKA GKK
Sbjct: 157 GTGVRDYIHVVDLADGHIAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKK 336
Query: 627 IPLVMGQRRPGDAEILFSS 571
IPLV RR GDAE ++++
Sbjct: 337 IPLVYAGRRAGDAETVYAA 393
Score = 104 bits (222), Expect(2) = 1e-028
Identities = 49/77 (63%), Positives = 54/77 (70%)
Frame = +2 / -1
Query: 584 ISASPGRRWPMTKGIFFPEAFSNALTISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCP 763
+SASP T+GIFFP+AFSNA TIS+TDVPFPVPRLY SHP+L E S SFL A P
Sbjct: 380 VSASPALLPA*TRGIFFPQAFSNAATISSTDVPFPVPRLYTSHPILSESS*SFLKAAMWP 201
Query: 764 SARSTT*DIITNPCPIL 814
SARSTT P P L
Sbjct: 200 SARSTTWM*SRTPVPSL 150
Score = 89.0 bits (188), Expect(2) = 5e-027
Identities = 43/59 (72%), Positives = 44/59 (74%)
Frame = +3 / -1
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSPRYPLG*APTGLKYL 962
+*SRTPVPS V * P VS G LPTA C T GIRLLG P GSSP PLG*APTGLKYL
Sbjct: 179 M*SRTPVPSLVL*SVP*TVSAGRLPTATCWT*GIRLLGTPRGSSPM*PLG*APTGLKYL 3
Score = 52.4 bits (108), Expect(2) = 5e-027
Identities = 27/67 (40%), Positives = 43/67 (64%)
Frame = +1 / -3
Query: 583 NLSITRTPLAHDQRDFLSRSLLKCIDYLQH*CSFSGSKVVCFTSYVR*AFKKLP*CEKMS 762
+L I+ + + +QRDFLS SLL+C ++LQH S S +++V F Y F +LP ++
Sbjct: 381 SLGISSSSSSINQRDFLSASLLECGNHLQHGRSLSSAQIVHFAPYSVRIFVELPQGCDVA 202
Query: 763 FSKVNHM 783
S+VN+M
Sbjct: 201 VSQVNNM 181
Score = 42.3 bits (86), Expect(2) = 1e-028
Identities = 19/40 (47%), Positives = 27/40 (67%)
Frame = +2 / -3
Query: 845 RWPPANCNLLNEGHKVVRSSTWVFTKVSTGVSPNWIKVPK 964
R PP + +LL+ GH+VV S VF V+T VS N ++VP+
Sbjct: 120 RRPPPDSDLLDIGHQVVGDSKGVFPDVATRVSANRVEVPQ 1
>gb|BJ449925.1|BJ449925 BJ449925 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak27e10 5', mRNA sequence
Length = 588
Score = 197 bits (424), Expect(4) = 7e-060
Identities = 80/102 (78%), Positives = 87/102 (85%)
Frame = -3 / +3
Query: 717 IGCEAYNLGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWK 538
IGCE YNLGTGKGTSVLEIVNAFEKASGKKIPLV+GQRRPGDAEILF+ KAEREL+WK
Sbjct: 3 IGCEPYNLGTGKGTSVLEIVNAFEKASGKKIPLVVGQRRPGDAEILFAGTGKAERELNWK 182
Query: 537 AKYGITEMCGDLWNWASKSPYGYAASESPKLNGSSR*ATPIG 412
AKYGITEMC D WNWASK+PYGY + +S K NG+S * G
Sbjct: 183 AKYGITEMCRDQWNWASKNPYGYGSPDSTKQNGNSH*TEQRG 308
Score = 169 bits (363), Expect(4) = 2e-048
Identities = 75/97 (77%), Positives = 76/97 (78%)
Frame = +2 / -3
Query: 428 QRELPFSFGDSDAAYP*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRR 607
Q ELPF +S YP*G LLAQFH S HISVMPYFAFQ SSLSA KRISASPGRR
Sbjct: 292 QCELPFCLVESGDPYP*GFLLAQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR 113
Query: 608 WPMTKGIFFPEAFSNALTISNTDVPFPVPRLYASHPM 718
P T GIFFPEAFSNA TISNTDVPFPVPRLY SHPM
Sbjct: 112 *PTTSGIFFPEAFSNAFTISNTDVPFPVPRLYGSHPM 2
Score = 130 bits (278), Expect(4) = 5e-038
Identities = 58/98 (59%), Positives = 70/98 (71%)
Frame = -2 / +2
Query: 718 HRM*SIQPWNRKRNISVGDSQCI*EGFWKENPFGHGPTASW*C*DSLFIAGKSRERAPLE 539
HRM*+IQPWN KR++SVGDS+CI EGFWKENP G W* DSL SRER LE
Sbjct: 2 HRM*TIQPWNWKRDVSVGDSKCIREGFWKENPAGRRSAPPW*RRDSLCRNW*SRERTQLE 181
Query: 538 SEIWHHRNVRRPVELG*QEPLWIRGI*VSEAEW*FPLS 425
S++WHH +V+ PVELG*QEPL +R + +AE F L+
Sbjct: 182 SKVWHHGDVQGPVELG*QEPLRVRVTRLDQAERQFTLN 295
Score = 125 bits (268), Expect(3) = 2e-030
Identities = 59/92 (64%), Positives = 64/92 (69%)
Frame = +1 / -2
Query: 442 IQLRRLRCRVSIRALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQ 621
+ L R+ V +R LASPVP V AH DAI FPVE SLCF KENL +TR L DQ
Sbjct: 278 VLLGRVG*PVPVRVLASPVPLVPAHLRDAILCFPVEFSLCFTSSCKENLGVTRAALTDDQ 99
Query: 622 RDFLSRSLLKCIDYLQH*CSFSGSKVVCFTSY 717
RDFLSRSLL+CI YLQH* FS SKVV FTSY
Sbjct: 98 RDFLSRSLLECIYYLQH*RPFSSSKVVWFTSY 3
Score = 122 bits (261), Expect = 3e-027
Identities = 55/85 (64%), Positives = 61/85 (71%)
Frame = -1 / +1
Query: 716 *DVKHTTLEPEKEHQCWR*SMHLRRLLERKSLWSWANGVLVMLRFSFHRRQKQRESSTGK 537
*DV HTTLE EK QCWR* MH RRLLERKS WS + LV RFS KQRE+STGK
Sbjct: 4 *DVNHTTLELEKGRQCWR**MHSRRLLERKSRWSSVSAALVTPRFSLQELVKQRENSTGK 183
Query: 536 RNMASQKCAETCGTGLARALMDTRH 462
++MAS++CA T GTGLAR L T H
Sbjct: 184 QSMASRRCAGTSGTGLARTLTGTGH 258
Score = 110 bits (235), Expect(3) = 1e-024
Identities = 51/97 (52%), Positives = 60/97 (61%)
Frame = +3 / -1
Query: 429 SGNYHSASETQMPRIHKGSC*PSSTGLRTFL*CHIXXXXXXXXXXXPAMKRESQHHQDAV 608
S N SA +++ R KGSC*PSSTG T *CH ++RES+ HQ
Sbjct: 291 SVNCRSAWSSRVTRTRKGSC*PSSTGPCTSP*CHTLLSS*VLSLLYQFLQRESRRHQGGA 112
Query: 609 GP*PKGFSFQKPSQMH*LSPTLMFLFRFQGCMLHILC 719
P GFSFQKPS+MH LSPTL LF+FQGCM+HILC
Sbjct: 111 DRRPAGFSFQKPSRMHLLSPTLTSLFQFQGCMVHILC 1
Score = 32.2 bits (64), Expect(4) = 7e-060
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -2 / +3
Query: 382 *CQPTRINVDLVGRSYKEKFYRPRKEMLSCSS 287
*C I VDLVG+S + Y R+ +L CSS
Sbjct: 342 *CLRAGIGVDLVGQSSYRESYEIRERLLCCSS 437
Score = 29.5 bits (58), Expect(4) = 7e-060
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / +3
Query: 223 CYSQPANSILFL 188
CYSQPANSIL L
Sbjct: 513 CYSQPANSILLL 548
Score = 29.5 bits (58), Expect(4) = 5e-038
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -1 / +2
Query: 383 VVPTNQDKCRSSRSVI 336
+VPT+ D+CRSSR VI
Sbjct: 341 LVPTSWDRCRSSRPVI 388
Score = 29.5 bits (58), Expect(4) = 7e-060
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / +1
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 295 LNNADRSVWTHLYMQL 342
Score = 28.1 bits (55), Expect(4) = 2e-048
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2 / -1
Query: 389 HVHASPHGPIGVAQ 430
HV SPHGPI V Q
Sbjct: 336 HVQVSPHGPIRVVQ 295
Score = 28.1 bits (55), Expect(4) = 2e-048
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1 / -1
Query: 337 MTDRLDLHLSWLVGT 381
MT RLDLH S LVGT
Sbjct: 387 MTGRLDLHRSQLVGT 343
Score = 27.6 bits (54), Expect(4) = 5e-038
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / +2
Query: 429 *ATPIGPCGLACT 391
* T IGPCGL CT
Sbjct: 296 *TTRIGPCGLTCT 334
Score = 26.7 bits (52), Expect(4) = 5e-038
Identities = 10/12 (83%), Positives = 12/12 (100%)
Frame = -3 / +1
Query: 225 LAIVNQLIQFYF 190
LAIVNQLIQF++
Sbjct: 511 LAIVNQLIQFFY 546
Score = 26.3 bits (51), Expect(3) = 2e-030
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +1 / -3
Query: 385 VSCTCESTRPNRRCS 429
V+CT ESTR + RCS
Sbjct: 340 VACTGESTRTDPRCS 296
Score = 24.0 bits (46), Expect(4) = 2e-048
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1 / -1
Query: 196 ELN*LVDYSK 225
ELN*LVDYSK
Sbjct: 540 ELN*LVDYSK 511
Score = 22.6 bits (43), Expect(3) = 2e-030
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2 / -2
Query: 197 N*ISWLTIA 223
N*ISWLTIA
Sbjct: 539 N*ISWLTIA 513
Score = 22.1 bits (42), Expect(3) = 1e-024
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +3 / -2
Query: 384 SLMYMRVHTAQSALLS 431
S MY VHT +SAL S
Sbjct: 341 SCMYR*VHTDRSALFS 294
Score = 22.1 bits (42), Expect(3) = 1e-024
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = +3 / -3
Query: 186 SRNRIELAG*L*Q 224
S RIELAG*L*Q
Sbjct: 550 SSKRIELAG*L*Q 512
>gb|AV936911.1|AV936911 AV936911 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal8e17 3', mRNA sequence
Length = 719
Score = 198 bits (426), Expect(2) = 1e-056
Identities = 79/118 (66%), Positives = 94/118 (79%)
Frame = -3 / -2
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKK 628
G G HVVDLA+GH+ AL KL+E S IGCE YNLGTGKGTSVLE+V AFEKA GKK
Sbjct: 679 GTGVRDYIHVVDLADGHIAALXKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKK 500
Query: 627 IPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSPYGYAASES 454
IPLV RR GDAE ++++ AKAE+EL WKAK+G+ EMC DLWNWASK+PYGY +S++
Sbjct: 499 IPLVYAGRRAGDAETVYAATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSSDN 326
Score = 141 bits (303), Expect = 5e-033
Identities = 70/114 (61%), Positives = 75/114 (65%)
Frame = +2 / +3
Query: 473 P*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSN 652
P*G LLAQFHRS HIS P AF SS SA A +SASP T+GIFFP+AFSN
Sbjct: 345 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 524
Query: 653 ALTISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCPSARSTT*DIITNPCPIL 814
A TIS+TDVPFPVPRLY SHP+L E S S L A PSARSTT P P L
Sbjct: 525 AATISSTDVPFPVPRLYTSHPILSESS*SXLKAAMWPSARSTTWM*SRTPVPSL 686
Score = 42.8 bits (87), Expect(2) = 1e-056
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = -2 / -2
Query: 847 SLTIFGNNYATKDGTGVRDYI 785
+LT++G +Y TKDGTGVRDYI
Sbjct: 718 ALTVYGTDYNTKDGTGVRDYI 656
>gb|BQ766274.1|BQ766274 EBro08_SQ005_C17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ005_C17 5', mRNA sequence
Length = 639
Score = 180 bits (388), Expect(4) = 5e-055
Identities = 74/95 (77%), Positives = 81/95 (85%)
Frame = -3 / +1
Query: 696 LGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITE 517
LGTGKGTSVLEIVNAFEKASGKKIPLV+GQRRPGDAEILF+ KAEREL+WKAKYGITE
Sbjct: 1 LGTGKGTSVLEIVNAFEKASGKKIPLVVGQRRPGDAEILFAGTGKAERELNWKAKYGITE 180
Query: 516 MCGDLWNWASKSPYGYAASESPKLNGSSR*ATPIG 412
MC D WNWASK+PYGY + +S K NG+S * G
Sbjct: 181 MCRDQWNWASKNPYGYGSPDSTKQNGNSH*TEQRG 285
Score = 150 bits (323), Expect(4) = 5e-043
Identities = 68/89 (76%), Positives = 69/89 (77%)
Frame = +2 / -2
Query: 428 QRELPFSFGDSDAAYP*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRR 607
Q ELPF +S YP*G LLAQFH S HISVMPYFAFQ SSLSA KRISASPGRR
Sbjct: 269 QCELPFCLVESGDPYP*GFLLAQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR 90
Query: 608 WPMTKGIFFPEAFSNALTISNTDVPFPVP 694
P T GIFFPEAFSNA TISNTDVPFPVP
Sbjct: 89 *PTTSGIFFPEAFSNAFTISNTDVPFPVP 3
Score = 113 bits (242), Expect(4) = 3e-033
Identities = 51/90 (56%), Positives = 62/90 (68%)
Frame = -2 / +3
Query: 694 WNRKRNISVGDSQCI*EGFWKENPFGHGPTASW*C*DSLFIAGKSRERAPLESEIWHHRN 515
WN KR++SVGDS+CI EGFWKENP G W* DSL SRER LES++WHH +
Sbjct: 3 WNWKRDVSVGDSKCIREGFWKENPAGRRSAPPW*RGDSLCRNW*SRERTQLESKVWHHGD 182
Query: 514 VRRPVELG*QEPLWIRGI*VSEAEW*FPLS 425
V+ PVELG*QEPL +R + +AE F L+
Sbjct: 183 VQGPVELG*QEPLRVRVTRLDQAERQFTLN 272
Score = 112 bits (239), Expect(3) = 2e-026
Identities = 53/85 (62%), Positives = 58/85 (68%)
Frame = +1 / -1
Query: 442 IQLRRLRCRVSIRALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQ 621
+ L R+ V +R LASPVP V AH DAI FPVE SLCF KENL +TR L DQ
Sbjct: 255 VLLGRVG*PVPVRVLASPVPLVPAHLRDAILCFPVEFSLCFTSSCKENLRVTRAALTDDQ 76
Query: 622 RDFLSRSLLKCIDYLQH*CSFSGSK 696
RDFLSRSLL+CI YLQH* FS SK
Sbjct: 75 RDFLSRSLLECIYYLQH*RPFSSSK 1
Score = 109 bits (233), Expect = 2e-023
Identities = 49/78 (62%), Positives = 55/78 (70%)
Frame = -1 / +2
Query: 695 LEPEKEHQCWR*SMHLRRLLERKSLWSWANGVLVMLRFSFHRRQKQRESSTGKRNMASQK 516
LE EK QCWR* MH RRLLERKS WS + LV RFS KQRE+STGK++MAS++
Sbjct: 2 LELEKGRQCWR**MHSRRLLERKSRWSSVSAALVTRRFSLQELVKQRENSTGKQSMASRR 181
Query: 515 CAETCGTGLARALMDTRH 462
CA T GTGLAR L T H
Sbjct: 182 CAGTSGTGLARTLTGTGH 235
Score = 32.2 bits (64), Expect(4) = 5e-055
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -2 / +1
Query: 382 *CQPTRINVDLVGRSYKEKFYRPRKEMLSCSS 287
*C I VDLVG+S + Y R+ +L CSS
Sbjct: 319 *CLRAGIGVDLVGQSSYRESYEIRERLLCCSS 414
Score = 29.5 bits (58), Expect(4) = 5e-055
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / +1
Query: 223 CYSQPANSILFL 188
CYSQPANSIL L
Sbjct: 490 CYSQPANSILLL 525
Score = 29.5 bits (58), Expect(4) = 3e-033
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -1 / +3
Query: 383 VVPTNQDKCRSSRSVI 336
+VPT+ D+CRSSR VI
Sbjct: 318 LVPTSWDRCRSSRPVI 365
Score = 29.5 bits (58), Expect(4) = 5e-055
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / +2
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 272 LNNADRSVWTHLYMQL 319
Score = 28.1 bits (55), Expect(4) = 5e-043
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2 / -3
Query: 389 HVHASPHGPIGVAQ 430
HV SPHGPI V Q
Sbjct: 313 HVQVSPHGPIRVVQ 272
Score = 28.1 bits (55), Expect(4) = 5e-043
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1 / -3
Query: 337 MTDRLDLHLSWLVGT 381
MT RLDLH S LVGT
Sbjct: 364 MTGRLDLHRSQLVGT 320
Score = 27.6 bits (54), Expect(4) = 3e-033
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / +3
Query: 429 *ATPIGPCGLACT 391
* T IGPCGL CT
Sbjct: 273 *TTRIGPCGLTCT 311
Score = 26.7 bits (52), Expect(4) = 3e-033
Identities = 10/12 (83%), Positives = 12/12 (100%)
Frame = -3 / +2
Query: 225 LAIVNQLIQFYF 190
LAIVNQLIQF++
Sbjct: 488 LAIVNQLIQFFY 523
Score = 26.3 bits (51), Expect(3) = 2e-026
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +1 / -2
Query: 385 VSCTCESTRPNRRCS 429
V+CT ESTR + RCS
Sbjct: 317 VACTGESTRTDPRCS 273
Score = 24.0 bits (46), Expect(4) = 5e-043
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1 / -3
Query: 196 ELN*LVDYSK 225
ELN*LVDYSK
Sbjct: 517 ELN*LVDYSK 488
Score = 22.6 bits (43), Expect(3) = 2e-026
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2 / -1
Query: 197 N*ISWLTIA 223
N*ISWLTIA
Sbjct: 516 N*ISWLTIA 490
>gb|BJ466608.1|BJ466608 BJ466608 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32p11 3', mRNA sequence
Length = 667
Score = 171 bits (367), Expect(4) = 3e-052
Identities = 70/91 (76%), Positives = 77/91 (84%)
Frame = -3 / -3
Query: 684 KGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGD 505
KGTSVLEIVNAFEKASGKKIPLV+GQRRPGDAEILF+ KAEREL+WKAKYGITEMC D
Sbjct: 665 KGTSVLEIVNAFEKASGKKIPLVVGQRRPGDAEILFAGTGKAERELNWKAKYGITEMCRD 486
Query: 504 LWNWASKSPYGYAASESPKLNGSSR*ATPIG 412
WNWASK+PYGY + +S K NG+S * G
Sbjct: 485 QWNWASKNPYGYGSPDSTKQNGNSH*TEQRG 393
Score = 142 bits (305), Expect(4) = 1e-040
Identities = 65/86 (75%), Positives = 66/86 (76%)
Frame = +2 / +1
Query: 428 QRELPFSFGDSDAAYP*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRR 607
Q ELPF +S YP*G LLAQFH S HISVMPYFAFQ SSLSA KRISASPGRR
Sbjct: 409 QCELPFCLVESGDPYP*GFLLAQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR 588
Query: 608 WPMTKGIFFPEAFSNALTISNTDVPF 685
P T GIFFPEAFSNA TISNTDVPF
Sbjct: 589 *PTTSGIFFPEAFSNAFTISNTDVPF 666
Score = 107 bits (228), Expect(4) = 2e-031
Identities = 49/87 (56%), Positives = 60/87 (68%)
Frame = -2 / -2
Query: 685 KRNISVGDSQCI*EGFWKENPFGHGPTASW*C*DSLFIAGKSRERAPLESEIWHHRNVRR 506
KR++SVGDS+CI EGFWKENP G W* DSL SRER LES++WHH +V+
Sbjct: 666 KRDVSVGDSKCIREGFWKENPAGRRSAPPW*RGDSLCRNW*SRERTQLESKVWHHGDVQG 487
Query: 505 PVELG*QEPLWIRGI*VSEAEW*FPLS 425
PVELG*QEPL +R + +AE F L+
Sbjct: 486 PVELG*QEPLRVRVTRLDQAERQFTLN 406
Score = 106 bits (227), Expect = 2e-022
Identities = 47/75 (62%), Positives = 53/75 (70%)
Frame = -1 / -1
Query: 686 EKEHQCWR*SMHLRRLLERKSLWSWANGVLVMLRFSFHRRQKQRESSTGKRNMASQKCAE 507
EK QCWR* MH RRLLERKS WS + LV RFS KQRE+STGK++MAS++CA
Sbjct: 667 EKGRQCWR**MHSRRLLERKSRWSSVSAALVTRRFSLQELVKQRENSTGKQSMASRRCAG 488
Query: 506 TCGTGLARALMDTRH 462
T GTGLAR L T H
Sbjct: 487 TSGTGLARTLTGTGH 443
Score = 106 bits (226), Expect(3) = 9e-025
Identities = 50/81 (61%), Positives = 55/81 (67%)
Frame = +1 / +3
Query: 442 IQLRRLRCRVSIRALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQ 621
+ L R+ V +R LASPVP V AH DAI FPVE SLCF KENL +TR L DQ
Sbjct: 423 VLLGRVG*PVPVRVLASPVPLVPAHLRDAILCFPVEFSLCFTSSCKENLRVTRAALTDDQ 602
Query: 622 RDFLSRSLLKCIDYLQH*CSF 684
RDFLSRSLL+CI YLQH* F
Sbjct: 603 RDFLSRSLLECIYYLQH*RPF 665
Score = 32.2 bits (64), Expect(4) = 3e-052
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -2 / -3
Query: 382 *CQPTRINVDLVGRSYKEKFYRPRKEMLSCSS 287
*C I VDLVG+S + Y R+ +L CSS
Sbjct: 359 *CLRAGIGVDLVGQSSYRESYEIRERLLCCSS 264
Score = 29.5 bits (58), Expect(4) = 3e-052
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / -3
Query: 223 CYSQPANSILFL 188
CYSQPANSIL L
Sbjct: 188 CYSQPANSILLL 153
Score = 29.5 bits (58), Expect(4) = 2e-031
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -1 / -2
Query: 383 VVPTNQDKCRSSRSVI 336
+VPT+ D+CRSSR VI
Sbjct: 360 LVPTSWDRCRSSRPVI 313
Score = 29.5 bits (58), Expect(4) = 3e-052
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / -1
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 406 LNNADRSVWTHLYMQL 359
Score = 28.1 bits (55), Expect(4) = 1e-040
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2 / +2
Query: 389 HVHASPHGPIGVAQ 430
HV SPHGPI V Q
Sbjct: 365 HVQVSPHGPIRVVQ 406
Score = 28.1 bits (55), Expect(4) = 1e-040
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1 / +2
Query: 337 MTDRLDLHLSWLVGT 381
MT RLDLH S LVGT
Sbjct: 314 MTGRLDLHRSQLVGT 358
Score = 27.6 bits (54), Expect(4) = 2e-031
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -2
Query: 429 *ATPIGPCGLACT 391
* T IGPCGL CT
Sbjct: 405 *TTRIGPCGLTCT 367
Score = 26.7 bits (52), Expect(4) = 2e-031
Identities = 10/12 (83%), Positives = 12/12 (100%)
Frame = -3 / -1
Query: 225 LAIVNQLIQFYF 190
LAIVNQLIQF++
Sbjct: 190 LAIVNQLIQFFY 155
Score = 26.3 bits (51), Expect(3) = 9e-025
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +1 / +1
Query: 385 VSCTCESTRPNRRCS 429
V+CT ESTR + RCS
Sbjct: 361 VACTGESTRTDPRCS 405
Score = 24.0 bits (46), Expect(4) = 1e-040
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1 / +2
Query: 196 ELN*LVDYSK 225
ELN*LVDYSK
Sbjct: 161 ELN*LVDYSK 190
Score = 22.6 bits (43), Expect(3) = 9e-025
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2 / +3
Query: 197 N*ISWLTIA 223
N*ISWLTIA
Sbjct: 162 N*ISWLTIA 188
>gb|BJ457343.1|BJ457343 BJ457343 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak27e10 3', mRNA sequence
Length = 579
Score = 160 bits (345), Expect(4) = 3e-049
Identities = 66/88 (75%), Positives = 73/88 (82%)
Frame = -3 / -2
Query: 675 SVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWN 496
SVL IVNAFEKASGKKIPLV+GQRRPGDAEILF+ KAEREL+WKAKYGITEMC D WN
Sbjct: 578 SVLXIVNAFEKASGKKIPLVVGQRRPGDAEILFAGTGKAERELNWKAKYGITEMCRDQWN 399
Query: 495 WASKSPYGYAASESPKLNGSSR*ATPIG 412
WASK+PYGY + +S K NG+S * G
Sbjct: 398 WASKNPYGYGSPDSTKQNGNSH*TEQRG 315
Score = 133 bits (284), Expect(4) = 9e-038
Identities = 61/83 (73%), Positives = 62/83 (74%)
Frame = +2 / +1
Query: 428 QRELPFSFGDSDAAYP*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRR 607
Q ELPF +S YP*G LLAQFH S HISVMPYFAFQ SSLSA KRISASPGRR
Sbjct: 331 QCELPFCLVESGDPYP*GFLLAQFHWSLHISVMPYFAFQLSSLSALPVPAKRISASPGRR 510
Query: 608 WPMTKGIFFPEAFSNALTISNTD 676
P T GIFFPEAFSNA TI NTD
Sbjct: 511 *PTTSGIFFPEAFSNAFTIXNTD 579
Score = 105 bits (225), Expect(3) = 1e-024
Identities = 49/78 (62%), Positives = 54/78 (69%)
Frame = +1 / +3
Query: 442 IQLRRLRCRVSIRALASPVPQVSAHFCDAIFRFPVELSLCFCRR*KENLSITRTPLAHDQ 621
+ L R+ V +R LASPVP V AH DAI FPVE SLCF KENL +TR L DQ
Sbjct: 345 VLLGRVG*PVPVRVLASPVPLVPAHLRDAILCFPVEFSLCFTSSCKENLGVTRAALTDDQ 524
Query: 622 RDFLSRSLLKCIDYLQH* 675
RDFLSRSLL+CI YLQH*
Sbjct: 525 RDFLSRSLLECIYYLQH* 578
Score = 99.1 bits (210), Expect(4) = 6e-029
Identities = 46/84 (54%), Positives = 56/84 (66%)
Frame = -2 / -1
Query: 676 ISVGDSQCI*EGFWKENPFGHGPTASW*C*DSLFIAGKSRERAPLESEIWHHRNVRRPVE 497
+SV DS+CI EGFWKENP G W* DSL SRER LES++WHH +V+ PVE
Sbjct: 579 VSVXDSKCIREGFWKENPAGRRSAPPW*RRDSLCRNW*SRERTQLESKVWHHGDVQGPVE 400
Query: 496 LG*QEPLWIRGI*VSEAEW*FPLS 425
LG*QEPL +R + +AE F L+
Sbjct: 399 LG*QEPLRVRVTRLDQAERQFTLN 328
Score = 32.2 bits (64), Expect(4) = 3e-049
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -2 / -2
Query: 382 *CQPTRINVDLVGRSYKEKFYRPRKEMLSCSS 287
*C I VDLVG+S + Y R+ +L CSS
Sbjct: 281 *CLRAGIGVDLVGQSSYRESYEIRERLLCCSS 186
Score = 29.5 bits (58), Expect(4) = 3e-049
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / -2
Query: 223 CYSQPANSILFL 188
CYSQPANSIL L
Sbjct: 110 CYSQPANSILLL 75
Score = 29.5 bits (58), Expect(4) = 6e-029
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -1 / -1
Query: 383 VVPTNQDKCRSSRSVI 336
+VPT+ D+CRSSR VI
Sbjct: 282 LVPTSWDRCRSSRPVI 235
Score = 29.5 bits (58), Expect(4) = 3e-049
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / -3
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 328 LNNADRSVWTHLYMQL 281
Score = 28.1 bits (55), Expect(4) = 9e-038
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2 / +2
Query: 389 HVHASPHGPIGVAQ 430
HV SPHGPI V Q
Sbjct: 287 HVQVSPHGPIRVVQ 328
Score = 28.1 bits (55), Expect(4) = 9e-038
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1 / +2
Query: 337 MTDRLDLHLSWLVGT 381
MT RLDLH S LVGT
Sbjct: 236 MTGRLDLHRSQLVGT 280
Score = 27.6 bits (54), Expect(4) = 6e-029
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -1
Query: 429 *ATPIGPCGLACT 391
* T IGPCGL CT
Sbjct: 327 *TTRIGPCGLTCT 289
Score = 26.7 bits (52), Expect(4) = 6e-029
Identities = 10/12 (83%), Positives = 12/12 (100%)
Frame = -3 / -3
Query: 225 LAIVNQLIQFYF 190
LAIVNQLIQF++
Sbjct: 112 LAIVNQLIQFFY 77
Score = 26.3 bits (51), Expect(3) = 1e-024
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +1 / +1
Query: 385 VSCTCESTRPNRRCS 429
V+CT ESTR + RCS
Sbjct: 283 VACTGESTRTDPRCS 327
Score = 24.0 bits (46), Expect(4) = 9e-038
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1 / +2
Query: 196 ELN*LVDYSK 225
ELN*LVDYSK
Sbjct: 83 ELN*LVDYSK 112
Score = 22.6 bits (43), Expect(3) = 1e-024
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2 / +3
Query: 197 N*ISWLTIA 223
N*ISWLTIA
Sbjct: 84 N*ISWLTIA 110
>gb|CA021085.1|CA021085 HZ39B01r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ39B01
5-PRIME, mRNA sequence
Length = 539
Score = 186 bits (401), Expect = 2e-046
Identities = 72/104 (69%), Positives = 87/104 (83%)
Frame = -3 / +1
Query: 765 EGHLFALRKLFESSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAE 586
+GH+ ALRKL+E S IGCE YNLGTGKGTSVLE+V AFEKA GKKIPLV RR GDAE
Sbjct: 1 DGHIAALRKLYEDSDRIGCEVYNLGTGKGTSVLEMVAAFEKACGKKIPLVYAGRRAGDAE 180
Query: 585 ILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSPYGYAASES 454
++++ AKAE+EL WKAK+G+ EMC DLWNWASK+PYGY +S++
Sbjct: 181 TVYAATAKAEKELKWKAKFGVEEMCRDLWNWASKNPYGYGSSDN 312
Score = 132 bits (282), Expect = 4e-030
Identities = 62/97 (63%), Positives = 67/97 (69%)
Frame = +2 / -1
Query: 473 P*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSN 652
P*G LLAQFHRS HIS P AF SS SA A +SASP T+GIFFP+AFSN
Sbjct: 293 P*GFLLAQFHRSLHISSTPNLAFHLSSFSALAVAAYTVSASPALLPA*TRGIFFPQAFSN 114
Query: 653 ALTISNTDVPFPVPRLYASHPMLDELSKSFLNAKRCP 763
A TIS+TDVPFPVPRLY SHP+L E S SFL A P
Sbjct: 113 AATISSTDVPFPVPRLYTSHPILSESS*SFLKAAMWP 3
>gb|BI952869.1|BI952869 HVSMEm0008B04f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0008B04f, mRNA sequence
Length = 744
Score = 127 bits (273), Expect(3) = 4e-041
Identities = 48/62 (77%), Positives = 56/62 (90%)
Frame = -2 / +1
Query: 970 ILLRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATKDGTGVRD 791
IL+R FNPVGAHPSGY+GEDP G PNNLMP+VQQ+AVGRRP+LT++G +Y TKDGTGV D
Sbjct: 7 ILVRDFNPVGAHPSGYIGEDPLGVPNNLMPYVQQVAVGRRPALTVYGTDYNTKDGTGVPD 186
Query: 790 YI 785
YI
Sbjct: 187 YI 192
Score = 84.5 bits (178), Expect(3) = 9e-024
Identities = 42/62 (67%), Positives = 44/62 (70%)
Frame = +3 / -2
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSPRYPLG*APTGLKYLS 965
+*S TPVPS V * P VS G LPTA C T GIRLLG P GSSP PLG*APTGLK L+
Sbjct: 191 M*SGTPVPSLVL*SVP*TVSAGRLPTATCWT*GIRLLGTPRGSSPM*PLG*APTGLKSLT 12
Query: 966 KM 971
M
Sbjct: 11 SM 6
Score = 41.8 bits (85), Expect(3) = 4e-041
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = -3 / +1
Query: 807 GQGFVIISHVVDLAEGHLFALRKLFESSSNIG 712
G G HVVDL +GH+ ALRKL+++S IG
Sbjct: 169 GTGVPDYIHVVDLPDGHIAALRKLYQNSDRIG 264
Score = 37.3 bits (75), Expect(3) = 4e-041
Identities = 17/34 (50%), Positives = 20/34 (58%)
Frame = -3 / +1
Query: 693 GTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGD 592
G KGT VLE V F K GKKIP V ++ G+
Sbjct: 280 GPWKGTXVLEKVAPFNKGCGKKIPWVFPGKKTGN 381
Score = 35.9 bits (72), Expect(3) = 9e-024
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = +2 / -2
Query: 617 TKGIFFPEAFSNALTISNTDVPFPVP 694
T+GIFFP+ N T S T VPF P
Sbjct: 356 TQGIFFPQPLLNGATFSRTXVPFQGP 279
Score = 27.6 bits (54), Expect(3) = 9e-024
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = +2 / -2
Query: 713 PMLDELSKSFLNAKRCPSARSTT 781
P+L E SFL A PS RSTT
Sbjct: 263 PILSEFW*SFLKAAMWPSGRSTT 195
>gb|CA000191.1|CA000191 HS09J09u HS Hordeum vulgare subsp. vulgare cDNA clone HS09J09
3-PRIME, mRNA sequence
Length = 549
Score = 153 bits (328), Expect(2) = 8e-041
Identities = 59/87 (67%), Positives = 74/87 (85%)
Frame = -3 / -2
Query: 729 SSSNIGCEAYNLGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERE 550
++ +IGC AYNLGTG+GT+VLE+V+AFEKA GKKIP+ M RRPGD+E +++S AKAE+E
Sbjct: 485 ATPDIGCVAYNLGTGRGTTVLEMVSAFEKAYGKKIPVKMCPRRPGDSEQVYASTAKAEQE 306
Query: 549 LHWKAKYGITEMCGDLWNWASKSPYGY 469
L W+AKYGI EMC D WNWA K+PYGY
Sbjct: 305 LGWRAKYGIEEMCRDQWNWARKNPYGY 225
Score = 103 bits (219), Expect(2) = 2e-023
Identities = 50/82 (60%), Positives = 54/82 (65%)
Frame = +2 / +1
Query: 473 P*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSN 652
P G LAQFH S HIS +PY A Q SS SA A D S SPG + GIF P AFSN
Sbjct: 229 PYGFFLAQFHWSLHISSIPYLALQPSSCSALAVDAYTCSESPGLLGHIFTGIFLPYAFSN 408
Query: 653 ALTISNTDVPFPVPRLYASHPM 718
ALTIS+T VP PVPRLYA+ PM
Sbjct: 409 ALTISSTVVPRPVPRLYATQPM 474
Score = 34.5 bits (69), Expect(2) = 8e-041
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = -3 / -2
Query: 783 HVVDLAEGHLFALRKLF 733
HVVDLA+GH+ AL KLF
Sbjct: 536 HVVDLADGHIAALEKLF 486
Score = 25.8 bits (50), Expect(2) = 2e-023
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2 / +1
Query: 728 LSKSFLNAKRCPSARSTT 781
++KSF +A PSARSTT
Sbjct: 481 VAKSFSSAAMWPSARSTT 534
>gb|BU975384.1|BU975384 HB31F04r BC Hordeum vulgare subsp. vulgare cDNA clone HB31F04
5-PRIME, mRNA sequence
Length = 501
Score = 152 bits (327), Expect(2) = 4e-040
Identities = 56/69 (81%), Positives = 64/69 (92%)
Frame = -2 / +1
Query: 991 SDPEWNIILLRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNYATK 812
SDP+W IILLRYFNPVGAHPSGY+GEDP G PNNLMP+VQQ+AVGRRP+LT++G +Y TK
Sbjct: 250 SDPDWKIILLRYFNPVGAHPSGYIGEDPLGVPNNLMPYVQQVAVGRRPALTVYGTDYNTK 429
Query: 811 DGTGVRDYI 785
DGTGVRDYI
Sbjct: 430 DGTGVRDYI 456
Score = 106 bits (226), Expect = 2e-022
Identities = 51/69 (73%), Positives = 52/69 (75%)
Frame = +3 / -2
Query: 786 I*SRTPVPSFVA*LFPNIVSDGLLPTAIC*TKGIRLLGVPHGSSPRYPLG*APTGLKYLS 965
+*SRTPVPS V * P VS G LPTA C T GIRLLG P GSSP PLG*APTGLKYLS
Sbjct: 455 M*SRTPVPSLVL*SVP*TVSAGRLPTATCWT*GIRLLGTPRGSSPM*PLG*APTGLKYLS 276
Query: 966 KMIFHSGSE 992
MIF SGSE
Sbjct: 275 SMIFQSGSE 249
Score = 32.7 bits (65), Expect(2) = 4e-040
Identities = 14/23 (60%), Positives = 16/23 (69%)
Frame = -3 / +1
Query: 807 GQGFVIISHVVDLAEGHLFALRK 739
G G HVVDLA+GH+ ALRK
Sbjct: 433 GTGVRDYIHVVDLADGHIAALRK 501
>gb|AJ462109.1|AJ462109 AJ462109 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200070B09F1, mRNA sequence
Length = 360
Score = 150 bits (322), Expect = 1e-035
Identities = 58/82 (70%), Positives = 70/82 (85%)
Frame = -3 / +1
Query: 714 GCEAYNLGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKA 535
GC AYNLGTG+GT+VLE+V+AFEKA GKKIP+ M RRPGD+E +++S AKAE+EL W+A
Sbjct: 13 GCVAYNLGTGRGTTVLEMVSAFEKAYGKKIPVKMCPRRPGDSEQVYASTAKAEQELGWRA 192
Query: 534 KYGITEMCGDLWNWASKSPYGY 469
KYGI EMC D WNWA K+PYGY
Sbjct: 193 KYGIEEMCRDQWNWARKNPYGY 258
Score = 103 bits (219), Expect = 2e-021
Identities = 50/81 (61%), Positives = 54/81 (66%)
Frame = +2 / -2
Query: 473 P*GLLLAQFHRSPHISVMPYFAFQWSSLSAFAGDEKRISASPGRRWPMTKGIFFPEAFSN 652
P*G LAQFH S HIS +PY A Q SS SA A D S SPG + GIF P AFSN
Sbjct: 254 P*GFFLAQFHWSLHISSIPYLALQPSSCSALAVDAYTCSESPGLLGHIFTGIFLPYAFSN 75
Query: 653 ALTISNTDVPFPVPRLYASHP 715
ALTIS+T VP PVPRLYA+ P
Sbjct: 74 ALTISSTVVPRPVPRLYATQP 12
>gb|CA013755.1|CA013755 HT09G07r HT Hordeum vulgare subsp. vulgare cDNA clone HT09G07
5-PRIME, mRNA sequence
Length = 530
Score = 136 bits (292), Expect(2) = 2e-034
Identities = 48/72 (66%), Positives = 61/72 (84%)
Frame = -2 / +2
Query: 1000 YLHSDPEWNIILLRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNY 821
Y H++PEW ILLRYFNP+GAH SG +GEDP G PNNL+P++QQ+AVGRRP L ++G++Y
Sbjct: 269 YQHANPEWRTILLRYFNPIGAHQSGDIGEDPKGVPNNLLPYIQQVAVGRRPELNVYGHDY 448
Query: 820 ATKDGTGVRDYI 785
T+DGT VRDYI
Sbjct: 449 RTRDGTAVRDYI 484
Score = 29.9 bits (59), Expect(2) = 2e-034
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = -3 / +2
Query: 783 HVVDLAEGHLFALRK 739
HVVDLA+GH+ AL K
Sbjct: 485 HVVDLADGHIAALEK 529
>gb|BJ459872.1|BJ459872 BJ459872 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38j12 3', mRNA sequence
Length = 469
Score = 89.0 bits (188), Expect(4) = 4e-028
Identities = 34/50 (68%), Positives = 39/50 (78%)
Frame = -3 / -1
Query: 561 AERELHWKAKYGITEMCGDLWNWASKSPYGYAASESPKLNGSSR*ATPIG 412
AEREL+WKAKYGITEMC D WNWASK+PYGY + +S K NG+S * G
Sbjct: 469 AERELNWKAKYGITEMCRDQWNWASKNPYGYGSPDSTKQNGNSH*TEQRG 320
Score = 32.2 bits (64), Expect(4) = 4e-028
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -2 / -1
Query: 382 *CQPTRINVDLVGRSYKEKFYRPRKEMLSCSS 287
*C I VDLVG+S + Y R+ +L CSS
Sbjct: 286 *CLRAGIGVDLVGQSSYRESYEIRERLLCCSS 191
Score = 29.5 bits (58), Expect(4) = 4e-028
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / -1
Query: 223 CYSQPANSILFL 188
CYSQPANSIL L
Sbjct: 115 CYSQPANSILLL 80
Score = 29.5 bits (58), Expect(4) = 4e-028
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / -2
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 333 LNNADRSVWTHLYMQL 286
>gb|AV911022.1|AV911022 AV911022 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11l22 3', mRNA sequence
Length = 468
Score = 89.0 bits (188), Expect(4) = 4e-028
Identities = 34/50 (68%), Positives = 39/50 (78%)
Frame = -3 / -1
Query: 561 AERELHWKAKYGITEMCGDLWNWASKSPYGYAASESPKLNGSSR*ATPIG 412
AEREL+WKAKYGITEMC D WNWASK+PYGY + +S K NG+S * G
Sbjct: 468 AERELNWKAKYGITEMCRDQWNWASKNPYGYGSPDSTKQNGNSH*TEQRG 319
Score = 32.2 bits (64), Expect(4) = 4e-028
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -2 / -1
Query: 382 *CQPTRINVDLVGRSYKEKFYRPRKEMLSCSS 287
*C I VDLVG+S + Y R+ +L CSS
Sbjct: 285 *CLRAGIGVDLVGQSSYRESYEIRERLLCCSS 190
Score = 29.5 bits (58), Expect(4) = 4e-028
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / -1
Query: 223 CYSQPANSILFL 188
CYSQPANSIL L
Sbjct: 114 CYSQPANSILLL 79
Score = 29.5 bits (58), Expect(4) = 4e-028
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / -2
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 332 LNNADRSVWTHLYMQL 285
>gb|BM816328.1|BM816328 HC108D12_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC108D12_SK.ab1 similar to UDP-glucose
4-epimerase,Aldose 1-epimerase,, mRNA sequence
Length = 860
Score = 122 bits (261), Expect = 3e-027
Identities = 43/57 (75%), Positives = 52/57 (91%)
Frame = -2 / +3
Query: 991 SDPEWNIILLRYFNPVGAHPSGYLGEDPCGTPNNLMPFVQQIAVGRRPSLTIFGNNY 821
SDP+W IILLRYFNPVGAHPSGY+GEDP G PNNLMP+VQQ+AVG+ P+LT++G +Y
Sbjct: 690 SDPDWEIILLRYFNPVGAHPSGYIGEDPLGVPNNLMPYVQQVAVGKXPALTVYGTDY 860
>gb|BJ452323.1|BJ452323 BJ452323 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38j12 5', mRNA sequence
Length = 492
Score = 80.3 bits (169), Expect(4) = 1e-025
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = -3 / +3
Query: 549 LHWKAKYGITEMCGDLWNWASKSPYGYAASESPKLNGSSR*ATPIG 412
L+WKAKYGITEMC D WNWASK+PYGY + +S K NG+S * G
Sbjct: 3 LNWKAKYGITEMCRDQWNWASKNPYGYGSPDSTKQNGNSH*TEQRG 140
Score = 32.2 bits (64), Expect(4) = 1e-025
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -2 / +3
Query: 382 *CQPTRINVDLVGRSYKEKFYRPRKEMLSCSS 287
*C I VDLVG+S + Y R+ +L CSS
Sbjct: 174 *CLRAGIGVDLVGQSSYRESYEIRERLLCCSS 269
Score = 29.5 bits (58), Expect(4) = 1e-025
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / +3
Query: 223 CYSQPANSILFL 188
CYSQPANSIL L
Sbjct: 345 CYSQPANSILLL 380
Score = 29.5 bits (58), Expect(4) = 1e-025
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / +1
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 127 LNNADRSVWTHLYMQL 174
>gb|AV909139.1|AV909139 AV909139 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11l22 5', mRNA sequence
Length = 488
Score = 78.5 bits (165), Expect(4) = 4e-025
Identities = 29/45 (64%), Positives = 34/45 (75%)
Frame = -3 / +2
Query: 546 HWKAKYGITEMCGDLWNWASKSPYGYAASESPKLNGSSR*ATPIG 412
+WKAKYGITEMC D WNWASK+PYGY + +S K NG+S * G
Sbjct: 2 NWKAKYGITEMCRDQWNWASKNPYGYGSPDSTKQNGNSH*TEQRG 136
Score = 32.2 bits (64), Expect(4) = 4e-025
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = -2 / +2
Query: 382 *CQPTRINVDLVGRSYKEKFYRPRKEMLSCSS 287
*C I VDLVG+S + Y R+ +L CSS
Sbjct: 170 *CLRAGIGVDLVGQSSYRESYEIRERLLCCSS 265
Score = 29.5 bits (58), Expect(4) = 4e-025
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / +2
Query: 223 CYSQPANSILFL 188
CYSQPANSIL L
Sbjct: 341 CYSQPANSILLL 376
Score = 29.5 bits (58), Expect(4) = 4e-025
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = -2 / +3
Query: 430 LSNADWAVWTRMYMRL 383
L+NAD +VWT +YM+L
Sbjct: 123 LNNADRSVWTHLYMQL 170
>gb|AV928242.1|AV928242 AV928242 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd27i15 3', mRNA sequence
Length = 678
Score = 67.5 bits (141), Expect(3) = 2e-021
Identities = 28/58 (48%), Positives = 40/58 (68%)
Frame = -3 / -1
Query: 702 YNLGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKAKY 529
YN+GTG+G SV E V A +KA+G I + RRPGD ++S+PA+ REL+W A++
Sbjct: 360 YNVGTGRGRSVKEFVEACKKATGVDIKVHYFPRRPGDYAEVYSNPARINRELNWTAQH 187
Score = 36.8 bits (74), Expect(3) = 2e-021
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = -2 / -1
Query: 982 EWNIILLRYFNPVGAHPSGYLGEDP 908
+ ++++LRYFN +G+ P G LGE P
Sbjct: 636 DMSVMILRYFNVIGSDPEGRLGEAP 562
Score = 35.9 bits (72), Expect(3) = 2e-021
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -2 / -1
Query: 865 AVGRRPSLTIFGNNYATKDGTGVRDYI 785
A+G P L + G +Y T DGT VRDYI
Sbjct: 510 ALGIIPGLKVKGTDYETPDGTCVRDYI 430
>gb|BJ467826.1|BJ467826 BJ467826 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29p06 3', mRNA sequence
Length = 658
Score = 67.5 bits (141), Expect(3) = 2e-021
Identities = 28/58 (48%), Positives = 40/58 (68%)
Frame = -3 / -2
Query: 702 YNLGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKAKY 529
YN+GTG+G SV E V A +KA+G I + RRPGD ++S+PA+ REL+W A++
Sbjct: 363 YNVGTGRGRSVKEFVEACKKATGVDIKVHYFPRRPGDYAEVYSNPARINRELNWTAQH 190
Score = 36.8 bits (74), Expect(3) = 2e-021
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = -2 / -2
Query: 982 EWNIILLRYFNPVGAHPSGYLGEDP 908
+ ++++LRYFN +G+ P G LGE P
Sbjct: 639 DMSVMILRYFNVIGSDPEGRLGEAP 565
Score = 35.9 bits (72), Expect(3) = 2e-021
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -2 / -2
Query: 865 AVGRRPSLTIFGNNYATKDGTGVRDYI 785
A+G P L + G +Y T DGT VRDYI
Sbjct: 513 ALGIIPGLKVKGTDYETPDGTCVRDYI 433
>gb|BG368552.1|BG368552 HVSMEi0019L05f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0019L05f, mRNA sequence
Length = 618
Score = 67.5 bits (141), Expect(3) = 2e-021
Identities = 28/58 (48%), Positives = 40/58 (68%)
Frame = -3 / +2
Query: 702 YNLGTGKGTSVLEIVNAFEKASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKAKY 529
YN+GTG+G SV E V A +KA+G I + RRPGD ++S+PA+ REL+W A++
Sbjct: 335 YNVGTGRGRSVKEFVEACKKATGVDIKVDYFPRRPGDYAEVYSNPARINRELNWTAQH 508
Score = 36.3 bits (73), Expect(3) = 2e-021
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = -2 / +2
Query: 976 NIILLRYFNPVGAHPSGYLGEDP 908
++++LRYFN +G+ P G LGE P
Sbjct: 65 SVMILRYFNVIGSDPEGRLGEAP 133
Score = 35.9 bits (72), Expect(3) = 2e-021
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -2 / +2
Query: 865 AVGRRPSLTIFGNNYATKDGTGVRDYI 785
A+G P L + G +Y T DGT VRDYI
Sbjct: 185 ALGIIPGLKVKGTDYETPDGTCVRDYI 265
>gb|CA001439.1|CA001439 HS17O15u HS Hordeum vulgare subsp. vulgare cDNA clone HS17O15
3-PRIME, mRNA sequence
Length = 550
Score = 102 bits (218), Expect = 3e-021
Identities = 39/58 (67%), Positives = 47/58 (81%)
Frame = -3 / -2
Query: 642 ASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSPYGY 469
A GKKIP+ M RRPGD+E +++S AKAE+EL W+AKYGI EMC D WNWA K+PYGY
Sbjct: 549 AYGKKIPVKMCPRRPGDSEQVYASTAKAEQELGWRAKYGIEEMCRDQWNWARKNPYGY 376
>gb|CA010057.1|CA010057 HT11K20u HT Hordeum vulgare subsp. vulgare cDNA clone HT11K20
3-PRIME, mRNA sequence
Length = 356
Score = 102 bits (218), Expect = 3e-021
Identities = 39/58 (67%), Positives = 47/58 (81%)
Frame = -3 / -1
Query: 642 ASGKKIPLVMGQRRPGDAEILFSSPAKAERELHWKAKYGITEMCGDLWNWASKSPYGY 469
A GKKIP+ M RRPGD+E +++S AKAE+EL W+AKYGI EMC D WNWA K+PYGY
Sbjct: 356 AYGKKIPVKMCPRRPGDSEQVYASTAKAEQELGWRAKYGIEEMCRDQWNWARKNPYGY 183
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 461,637,349
Number of Sequences: 312970
Number of extensions: 7483021
Number of successful extensions: 39934
Number of sequences better than 1.0e-020: 41
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 12083773093
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)