TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3802297.2.1
         (606 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BE422260.1|BE422260  HWM022cC.01r ITEC HWM Barley Leaf Li...   130   6e-030
gb|BI950935.1|BI950935  HVSMEl0023L12f Hordeum vulgare spike...   120   6e-027
gb|BQ765469.1|BQ765469  EBro03_SQ007_C09_R root, 3 week, wat...   120   8e-027
gb|BJ468665.1|BJ468665  BJ468665 K. Sato unpublished cDNA li...   111   3e-024
gb|BM374246.2|BM374246  EBpi03_SQ003_B10_R pistil, 4 DPA, no...   109   2e-023

>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
           vulgare cDNA clone HWM022cC.01, mRNA sequence
          Length = 1239

 Score =  130 bits (279), Expect = 6e-030
 Identities = 51/109 (46%), Positives = 67/109 (61%)
 Frame = +2 / +3

                                                                       
Query: 101 RTFLGRPWKEHSRTLYIQSEIGGFIDPQGWLPWLGDFGLSTCYYAKVENHGPGANMSERV 280
           R++LGRPWK++SRT+++QSE+   ++P GWL W G F L T YY + +N GPGA  S RV
Sbjct: 114 RSYLGRPWKQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRV 293

                                                            
Query: 281 KWRGIKNITYQHAMQKYTVESFIQGQHWLPQLGVPFIPGLLPQQQSGRI 427
            W+G + IT       +TV SFI G  WL    +PF  GL P   S  I
Sbjct: 294 TWKGYRVITSASEASTFTVGSFIDGDVWLAGTSIPFSAGL*PTNSSSII 440



>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
           (Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
           clone HVSMEl0023L12f, mRNA sequence
          Length = 536

 Score =  120 bits (257), Expect = 6e-027
 Identities = 48/106 (45%), Positives = 64/106 (60%)
 Frame = +2 / +2

                                                                       
Query: 2   AQGRKERRSVGGTVIHNCTVAPHPEFEKSVGRFRTFLGRPWKEHSRTLYIQSEIGGFIDP 181
           A GR +     G VI NC + P  +      +  ++LGRPWKE SR + ++S+I  FI P
Sbjct: 212 AHGRTDPNMKSGLVIQNCRLVPDQKLFVDRFKIPSYLGRPWKEFSRLVIMESQIADFIKP 391

                                                         
Query: 182 QGWLPWLGDFGLSTCYYAKVENHGPGANMSERVKWRGIKNITYQHA 319
           +G++PW GDFGL T YYA+  N GPGA  S+RV W G + I  Q A
Sbjct: 392 EGYMPWNGDFGLKTLYYAEYGNRGPGAGTSKRVTWPGFRVIARQEA 529



>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
           Hordeum vulgare subsp. vulgare cDNA clone
           EBro03_SQ007_C09 5', mRNA sequence
          Length = 417

 Score =  120 bits (256), Expect = 8e-027
 Identities = 44/93 (47%), Positives = 59/93 (63%)
 Frame = +2 / +2

                                                                       
Query: 122 WKEHSRTLYIQSEIGGFIDPQGWLPWLGDFGLSTCYYAKVENHGPGANMSERVKWRGIKN 301
           WK +SRT+ +QS++ G +DP GW+PW GDF LST YYA+ +N G G++ S RV W G   
Sbjct: 2   WKMYSRTVIMQSDVAGIVDPAGWMPWSGDFALSTLYYAEYDNSGAGSDTSRRVNWPGYHV 181

                                            
Query: 302 ITYQHAMQKYTVESFIQGQHWLPQLGVPFIPGL 400
           +        +TV + + G  WLPQ GVPF  GL
Sbjct: 182 LNSTVDAGNFTVANMVLGDFWLPQTGVPFTVGL 280



>gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA library, cv. Haruna Nijo
           germination shoots Hordeum vulgare subsp. vulgare cDNA
           clone bags39m02 3', mRNA sequence
          Length = 606

 Score =  111 bits (237), Expect = 3e-024
 Identities = 45/101 (44%), Positives = 59/101 (58%)
 Frame = +2 / -3

                                                                       
Query: 125 KEHSRTLYIQSEIGGFIDPQGWLPWLGDFGLSTCYYAKVENHGPGANMSERVKWRGIKNI 304
           K++SRT+++QSE+   ++P GWL W G F L T YY + +N GPGA  S RV W+G + I
Sbjct: 604 KQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVI 425

                                                    
Query: 305 TYQHAMQKYTVESFIQGQHWLPQLGVPFIPGLLPQQQSGRI 427
           T       +TV SFI G  WL    +PF  GL P   S  I
Sbjct: 424 TSASEASTFTVGSFIDGDVWLAGTSIPFSAGL*PTNSSSII 302



>gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
           Hordeum vulgare subsp. vulgare cDNA clone
           EBpi03_SQ003_B10 5', mRNA sequence
          Length = 449

 Score =  109 bits (232), Expect = 2e-023
 Identities = 44/100 (44%), Positives = 58/100 (58%)
 Frame = +2 / +2

                                                                       
Query: 128 EHSRTLYIQSEIGGFIDPQGWLPWLGDFGLSTCYYAKVENHGPGANMSERVKWRGIKNIT 307
           ++SRT+++QSE+   ++P GWL W G F L T YY + +N GPGA  S RV W+G + IT
Sbjct: 2   QYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVIT 181

                                                   
Query: 308 YQHAMQKYTVESFIQGQHWLPQLGVPFIPGLLPQQQSGRI 427
                  +TV SFI G  WL    +PF  GL P   S  I
Sbjct: 182 SASEASTFTVGSFIDGDVWLAGTSIPFSAGL*PTNSSSII 301



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 277,218,621
Number of Sequences: 312970
Number of extensions: 4873860
Number of successful extensions: 90343
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6362506350
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)