TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3748535.2.1
(619 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV932909.1|AV932909 AV932909 K. Sato unpublished cDNA li... 226 5e-075
gb|BJ472039.1|BJ472039 BJ472039 K. Sato unpublished cDNA li... 226 4e-071
gb|CA028274.1|CA028274 HZ61I02r HZ Hordeum vulgare subsp. v... 205 9e-069
gb|AV932668.1|AV932668 AV932668 K. Sato unpublished cDNA li... 226 4e-067
gb|CK122610.1|CK122610 BES1824104j23 BES1824 Hordeum vulgar... 172 8e-064
gb|BU990099.1|BU990099 HF24B10r HF Hordeum vulgare subsp. v... 221 2e-057
gb|BG300712.2|BG300712 HVSMEb0018B21f Hordeum vulgare seedl... 161 1e-055
gb|AL507698.1|AL507698 AL507698 Hordeum vulgare Barke devel... 147 4e-055
gb|BU984788.1|BU984788 HF05A10r HF Hordeum vulgare subsp. v... 143 4e-050
gb|CA012169.1|CA012169 HT04K11r HT Hordeum vulgare subsp. v... 143 4e-050
gb|AV932284.1|AV932284 AV932284 K. Sato unpublished cDNA li... 143 4e-050
gb|AV917169.1|AV917169 AV917169 K. Sato unpublished cDNA li... 91 8e-045
gb|BE215659.3|BE215659 HV_CEb0007L14f Hordeum vulgare seedl... 124 1e-044
gb|BQ472834.1|BQ472834 HB01F10r BC Hordeum vulgare subsp. v... 154 4e-037
gb|BF257939.2|BF257939 HVSMEf0014E17f Hordeum vulgare seedl... 139 1e-032
gb|CA028533.1|CA028533 HZ62F08r HZ Hordeum vulgare subsp. v... 139 1e-032
gb|BQ468511.1|BQ468511 HM01H13T HM Hordeum vulgare subsp. v... 75 5e-030
gb|BU975772.1|BU975772 HB32E04r BC Hordeum vulgare subsp. v... 128 3e-029
gb|BF619597.3|BF619597 HVSMEc0004K03f Hordeum vulgare seedl... 115 2e-025
gb|BF266844.2|BF266844 HV_CEa0016D13f Hordeum vulgare seedl... 110 7e-024
gb|BU971669.1|BU971669 HB19E23r BC Hordeum vulgare subsp. v... 107 5e-023
>gb|AV932909.1|AV932909 AV932909 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal4j24 5', mRNA sequence
Length = 677
Score = 226 bits (489), Expect(3) = 5e-075
Identities = 93/122 (76%), Positives = 102/122 (83%)
Frame = +2 / +3
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 246 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 425
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I+RGKEL S GLS C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YRVL
Sbjct: 426 ITRGKELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVL 605
Query: 614 KP 619
KP
Sbjct: 606 KP 611
Score = 148 bits (317), Expect(3) = 6e-042
Identities = 67/120 (55%), Positives = 81/120 (67%)
Frame = -2 / -2
Query: 618 GFSTR*TSL*TPCASGXXXXXXXXXXASKVLSDIGMSMKSPFKKQHCSLKPAEEMSSLPL 439
GF+TR* SL* P SG ASKVLS+ G+ MKS K L P + +SS PL
Sbjct: 610 GFNTR*ISL*QPTGSGAWHVASMA*TASKVLSENGIFMKSALTKSQVPLSPTDRLSSFPL 431
Query: 438 EMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMASREVSP 259
+W*SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP + +C A+KC CRL+ S + SP
Sbjct: 430 VIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCRARKCSCRLILSLKDSP 251
Score = 56.5 bits (117), Expect(3) = 5e-075
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +3
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 21 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 119
Score = 36.8 bits (74), Expect(3) = 5e-075
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +3
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 126 YGGKEESRKSNYTDMVN 176
Score = 35.9 bits (72), Expect(3) = 6e-042
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -2
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 115 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 26
Score = 24.9 bits (48), Expect(3) = 6e-042
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -2
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 166 SV*LDFLDSSFPP 128
>gb|BJ472039.1|BJ472039 BJ472039 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal31k11 5', mRNA sequence
Length = 628
Score = 226 bits (489), Expect(3) = 4e-071
Identities = 93/122 (76%), Positives = 102/122 (83%)
Frame = +2 / +1
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 220 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 399
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I+RGKEL S GLS C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YRVL
Sbjct: 400 ITRGKELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVL 579
Query: 614 KP 619
KP
Sbjct: 580 KP 585
Score = 148 bits (317), Expect(3) = 4e-040
Identities = 67/120 (55%), Positives = 81/120 (67%)
Frame = -2 / -3
Query: 618 GFSTR*TSL*TPCASGXXXXXXXXXXASKVLSDIGMSMKSPFKKQHCSLKPAEEMSSLPL 439
GF+TR* SL* P SG ASKVLS+ G+ MKS K L P + +SS PL
Sbjct: 584 GFNTR*ISL*QPTGSGAWHVASMA*TASKVLSENGIFMKSALTKSQVPLSPTDRLSSFPL 405
Query: 438 EMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMASREVSP 259
+W*SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP + +C A+KC CRL+ S + SP
Sbjct: 404 VIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCRARKCSCRLILSLKDSP 225
Score = 43.2 bits (88), Expect(3) = 4e-071
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = +3 / +1
Query: 126 LARSTGGSISKDQVKSAVDQYVKYH 200
LA GG I K+QVKSAVD+Y KYH
Sbjct: 19 LASGLGGKIDKEQVKSAVDEYEKYH 93
Score = 36.8 bits (74), Expect(3) = 4e-071
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 100 YGGKEESRKSNYTDMVN 150
Score = 29.5 bits (58), Expect(3) = 4e-040
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -1 / -3
Query: 196 YLTY*STADLTWSLLIDPP 140
Y +Y STADLT SL I PP
Sbjct: 89 YFSYSSTADLTCSLSIFPP 33
Score = 24.9 bits (48), Expect(3) = 4e-040
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -3
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 140 SV*LDFLDSSFPP 102
>gb|CA028274.1|CA028274 HZ61I02r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ61I02
5-PRIME, mRNA sequence
Length = 678
Score = 205 bits (443), Expect(3) = 9e-069
Identities = 85/111 (76%), Positives = 93/111 (83%)
Frame = +2 / +1
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 346 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 525
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQG 586
I+RGKEL S GLS C F+K DFM MP SDNTFDA YAI+ATCHAPD G
Sbjct: 526 ITRGKELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVG 678
Score = 133 bits (286), Expect(3) = 9e-038
Identities = 59/109 (54%), Positives = 72/109 (66%)
Frame = -2 / -2
Query: 585 PCASGXXXXXXXXXXASKVLSDIGMSMKSPFKKQHCSLKPAEEMSSLPLEMW*SLLFSPV 406
P SG ASKVLS+ G+ MKS K L P + +SS PL +W*SLLF+PV
Sbjct: 677 PTGSGAWHVASMA*TASKVLSENGIFMKSALTKSQVPLSPTDRLSSFPLVIW*SLLFNPV 498
Query: 405 IDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMASREVSP 259
+VELNLAIS+ GPPIP PTSKTFIP + +C A+KC CRL+ S + SP
Sbjct: 497 TEVELNLAISLNGPPIPQPTSKTFIPGFNSSCRARKCSCRLILSLKDSP 351
Score = 56.5 bits (117), Expect(3) = 9e-069
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +1
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 121 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 219
Score = 36.8 bits (74), Expect(3) = 9e-069
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 226 YGGKEESRKSNYTDMVN 276
Score = 35.9 bits (72), Expect(3) = 9e-038
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -2
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 215 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 126
Score = 24.9 bits (48), Expect(3) = 9e-038
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -2
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 266 SV*LDFLDSSFPP 228
>gb|AV932668.1|AV932668 AV932668 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal1j14 5', mRNA sequence
Length = 580
Score = 226 bits (489), Expect(3) = 4e-067
Identities = 93/122 (76%), Positives = 102/122 (83%)
Frame = +2 / +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 167 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 346
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVL 613
I+RGKEL S GLS C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YRVL
Sbjct: 347 ITRGKELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVL 526
Query: 614 KP 619
KP
Sbjct: 527 KP 532
Score = 148 bits (317), Expect(2) = 1e-036
Identities = 67/120 (55%), Positives = 81/120 (67%)
Frame = -2 / -2
Query: 618 GFSTR*TSL*TPCASGXXXXXXXXXXASKVLSDIGMSMKSPFKKQHCSLKPAEEMSSLPL 439
GF+TR* SL* P SG ASKVLS+ G+ MKS K L P + +SS PL
Sbjct: 531 GFNTR*ISL*QPTGSGAWHVASMA*TASKVLSENGIFMKSALTKSQVPLSPTDRLSSFPL 352
Query: 438 EMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMASREVSP 259
+W*SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP + +C A+KC CRL+ S + SP
Sbjct: 351 VIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCRARKCSCRLILSLKDSP 172
Score = 36.8 bits (74), Expect(3) = 4e-067
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +2
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 47 YGGKEESRKSNYTDMVN 97
Score = 29.9 bits (59), Expect(3) = 4e-067
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +3 / +2
Query: 162 QVKSAVDQYVKYH 200
QVKSAVD+Y KYH
Sbjct: 2 QVKSAVDEYEKYH 40
Score = 24.9 bits (48), Expect(2) = 1e-036
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -2
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 87 SV*LDFLDSSFPP 49
>gb|CK122610.1|CK122610 BES1824104j23 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010J234 5-PRIME, mRNA sequence
Length = 730
Score = 172 bits (370), Expect(4) = 8e-064
Identities = 72/94 (76%), Positives = 79/94 (84%)
Frame = +2 / +1
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 343 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 522
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISDNTF 535
I+RGKEL S GLS C F+K DFM MP SDNTF
Sbjct: 523 ITRGKELNRSVGLSGTCDFVKADFMKMPFSDNTF 624
Score = 128 bits (275), Expect(3) = 3e-036
Identities = 54/92 (58%), Positives = 67/92 (72%)
Frame = -2 / -3
Query: 534 KVLSDIGMSMKSPFKKQHCSLKPAEEMSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIP 355
KVLS+ G+ MKS K L P + +SS PL +W*SLLF+PV +VELNLAIS+ GPPIP
Sbjct: 623 KVLSENGIFMKSALTKSQVPLSPTDRLSSFPLVIW*SLLFNPVTEVELNLAISLNGPPIP 444
Query: 354 HPTSKTFIPFLSPNCSAKKCWCRLMASREVSP 259
PTSKTFIP + +C A+KC CRL+ S + SP
Sbjct: 443 QPTSKTFIPGFNSSCRARKCSCRLILSLKDSP 348
Score = 56.5 bits (117), Expect(4) = 8e-064
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +1
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 118 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 216
Score = 36.8 bits (74), Expect(4) = 8e-064
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 223 YGGKEESRKSNYTDMVN 273
Score = 35.9 bits (72), Expect(3) = 3e-036
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -3
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 212 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 123
Score = 35.0 bits (70), Expect(4) = 8e-064
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +2 / +2
Query: 533 FDAAYAIQATCHAP 574
FDA YAI+ATCHAP
Sbjct: 623 FDAXYAIEATCHAP 664
Score = 24.9 bits (48), Expect(3) = 3e-036
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -3
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 263 SV*LDFLDSSFPP 225
>gb|BU990099.1|BU990099 HF24B10r HF Hordeum vulgare subsp. vulgare cDNA clone HF24B10
5-PRIME, mRNA sequence
Length = 592
Score = 221 bits (478), Expect = 2e-057
Identities = 91/116 (78%), Positives = 98/116 (84%)
Frame = +2 / +2
Query: 272 REAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHISRGKE 451
RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY I+RGKE
Sbjct: 2 RESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQITRGKE 181
Query: 452 LISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
L S GLS C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YRVLKP
Sbjct: 182 LNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKP 349
Score = 144 bits (310), Expect = 3e-034
Identities = 65/115 (56%), Positives = 78/115 (67%)
Frame = -2 / -2
Query: 618 GFSTR*TSL*TPCASGXXXXXXXXXXASKVLSDIGMSMKSPFKKQHCSLKPAEEMSSLPL 439
GF+TR* SL* P SG ASKVLS+ G+ MKS K L P + +SS PL
Sbjct: 348 GFNTR*ISL*QPTGSGAWHVASMA*TASKVLSENGIFMKSALTKSQVPLSPTDRLSSFPL 169
Query: 438 EMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMAS 274
+W*SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP + +C A+KC CRL+ S
Sbjct: 168 VIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCRARKCSCRLILS 4
>gb|BG300712.2|BG300712 HVSMEb0018B21f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0018B21f, mRNA sequence
Length = 601
Score = 161 bits (346), Expect(3) = 1e-055
Identities = 68/91 (74%), Positives = 75/91 (82%)
Frame = +2 / +1
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 328 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 507
Query: 434 ISRGKELISSAGLSEQCCFLKGDFMDMPISD 526
I+RGKEL S GLS C +K DFM MP SD
Sbjct: 508 ITRGKELNRSVGLSGTCDXVKADFMKMPFSD 600
Score = 120 bits (256), Expect(3) = 1e-033
Identities = 50/89 (56%), Positives = 63/89 (70%)
Frame = -2 / -3
Query: 525 SDIGMSMKSPFKKQHCSLKPAEEMSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIPHPT 346
S+ G+ MKS L P + +SS PL +W*SLLF+PV +VELNLAIS+ GPPIP PT
Sbjct: 599 SENGIFMKSALTXSQVPLSPTDRLSSFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPT 420
Query: 345 SKTFIPFLSPNCSAKKCWCRLMASREVSP 259
SKTFIP + +C A+KC CRL+ S + SP
Sbjct: 419 SKTFIPGFNSSCRARKCSCRLILSLKDSP 333
Score = 56.5 bits (117), Expect(3) = 1e-055
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +1
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 103 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 201
Score = 36.8 bits (74), Expect(3) = 1e-055
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 208 YGGKEESRKSNYTDMVN 258
Score = 35.9 bits (72), Expect(3) = 1e-033
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -3
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 197 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 108
Score = 24.9 bits (48), Expect(3) = 1e-033
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -3
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 248 SV*LDFLDSSFPP 210
>gb|AL507698.1|AL507698 AL507698 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY06J16V 5',
mRNA sequence
Length = 700
Score = 147 bits (315), Expect(4) = 4e-055
Identities = 62/82 (75%), Positives = 69/82 (84%)
Frame = +2 / +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 326 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 505
Query: 434 ISRGKELISSAGLSEQCCFLKG 499
I+RGKEL S GLS C F +G
Sbjct: 506 ITRGKELNRSVGLSGTCDFCQG 571
Score = 115 bits (246), Expect(4) = 3e-040
Identities = 47/80 (58%), Positives = 59/80 (73%)
Frame = -2 / -2
Query: 498 PFKKQHCSLKPAEEMSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLS 319
P +K L P + +SS PL +W*SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP +
Sbjct: 570 P*QKSQVPLSPTDRLSSFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFN 391
Query: 318 PNCSAKKCWCRLMASREVSP 259
+C A+KC CRL+ S + SP
Sbjct: 390 SSCRARKCSCRLILSLKDSP 331
Score = 83.5 bits (176), Expect(2) = 1e-024
Identities = 34/57 (59%), Positives = 39/57 (68%)
Frame = +2 / +3
Query: 446 KELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+ LI L E F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YR +K
Sbjct: 519 RSLIGL*DLVELVIFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRGIK 689
Score = 56.5 bits (117), Expect(4) = 4e-055
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +2
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 101 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 199
Score = 48.7 bits (100), Expect(2) = 1e-024
Identities = 22/50 (44%), Positives = 28/50 (56%)
Frame = +1 / +1
Query: 304 GTTIGAQEGYEGFGCGMWNWGTFNRNRQIQLNIDNWTEQQ*LPHLKGQGA 453
G T + EGFGC +WN RN +I L++ W EQQ LP G+GA
Sbjct: 376 GPTA*VETRDEGFGCRLWNRRAVKRNCEI*LHLSYWIEQQRLPDN*GKGA 525
Score = 44.1 bits (90), Expect(4) = 3e-040
Identities = 22/43 (51%), Positives = 25/43 (58%)
Frame = -1 / -3
Query: 616 FQHTVDLTIDALCIWCMTCSLYCIGRIKGVIRYWHVHEVPLQE 488
F TVDL I A I + C L I IK VIR WH+HEV L +
Sbjct: 689 FNTTVDLLIAANRIRRVACCLNGINSIKSVIREWHLHEVCLDK 561
Score = 36.8 bits (74), Expect(4) = 4e-055
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +2
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 206 YGGKEESRKSNYTDMVN 256
Score = 35.9 bits (72), Expect(4) = 3e-040
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -2
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 195 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 106
Score = 30.9 bits (61), Expect(4) = 4e-055
Identities = 18/38 (47%), Positives = 20/38 (52%)
Frame = +3 / +1
Query: 495 RGTSWTCQYLITPLMRPMQYRLHVMHQMHKASIVRSTV 608
R TS C LIT LM M R H + A+I RSTV
Sbjct: 568 RQTS*RCHSLITLLMLFMPLRQHATRLIRLAAIRRSTV 681
Score = 24.9 bits (48), Expect(4) = 3e-040
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -2
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 246 SV*LDFLDSSFPP 208
>gb|BU984788.1|BU984788 HF05A10r HF Hordeum vulgare subsp. vulgare cDNA clone HF05A10
5-PRIME, mRNA sequence
Length = 567
Score = 143 bits (306), Expect(3) = 4e-050
Identities = 60/77 (77%), Positives = 66/77 (85%)
Frame = +2 / +3
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 336 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 515
Query: 434 ISRGKELISSAGLSEQC 484
I+RGKEL S GLS C
Sbjct: 516 ITRGKELNRSVGLSGTC 566
Score = 112 bits (239), Expect(3) = 2e-031
Identities = 45/72 (62%), Positives = 56/72 (77%)
Frame = -2 / -3
Query: 474 LKPAEEMSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKC 295
L P + +SS PL +W*SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP + +C A+KC
Sbjct: 556 LSPTDRLSSFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCRARKC 377
Query: 294 WCRLMASREVSP 259
CRL+ S + SP
Sbjct: 376 SCRLILSLKDSP 341
Score = 56.5 bits (117), Expect(3) = 4e-050
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +3
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 111 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 209
Score = 36.8 bits (74), Expect(3) = 4e-050
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +3
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 216 YGGKEESRKSNYTDMVN 266
Score = 35.9 bits (72), Expect(3) = 2e-031
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -3
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 205 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 116
Score = 24.9 bits (48), Expect(3) = 2e-031
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -3
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 256 SV*LDFLDSSFPP 218
>gb|CA012169.1|CA012169 HT04K11r HT Hordeum vulgare subsp. vulgare cDNA clone HT04K11
5-PRIME, mRNA sequence
Length = 507
Score = 143 bits (306), Expect(3) = 4e-050
Identities = 60/77 (77%), Positives = 66/77 (85%)
Frame = +2 / +3
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 276 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 455
Query: 434 ISRGKELISSAGLSEQC 484
I+RGKEL S GLS C
Sbjct: 456 ITRGKELNRSVGLSGTC 506
Score = 112 bits (239), Expect(3) = 2e-031
Identities = 45/72 (62%), Positives = 56/72 (77%)
Frame = -2 / -3
Query: 474 LKPAEEMSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKC 295
L P + +SS PL +W*SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP + +C A+KC
Sbjct: 496 LSPTDRLSSFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCRARKC 317
Query: 294 WCRLMASREVSP 259
CRL+ S + SP
Sbjct: 316 SCRLILSLKDSP 281
Score = 56.5 bits (117), Expect(3) = 4e-050
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +3
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 51 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 149
Score = 36.8 bits (74), Expect(3) = 4e-050
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +3
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 156 YGGKEESRKSNYTDMVN 206
Score = 35.9 bits (72), Expect(3) = 2e-031
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -3
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 145 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 56
Score = 24.9 bits (48), Expect(3) = 2e-031
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -3
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 196 SV*LDFLDSSFPP 158
>gb|AV932284.1|AV932284 AV932284 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal2p18 5', mRNA sequence
Length = 469
Score = 143 bits (306), Expect(3) = 4e-050
Identities = 60/77 (77%), Positives = 66/77 (85%)
Frame = +2 / +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY
Sbjct: 236 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQ 415
Query: 434 ISRGKELISSAGLSEQC 484
I+RGKEL S GLS C
Sbjct: 416 ITRGKELNRSVGLSGTC 466
Score = 112 bits (239), Expect(3) = 2e-031
Identities = 45/72 (62%), Positives = 56/72 (77%)
Frame = -2 / -2
Query: 474 LKPAEEMSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKC 295
L P + +SS PL +W*SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP + +C A+KC
Sbjct: 456 LSPTDRLSSFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCRARKC 277
Query: 294 WCRLMASREVSP 259
CRL+ S + SP
Sbjct: 276 SCRLILSLKDSP 241
Score = 56.5 bits (117), Expect(3) = 4e-050
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +2
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 11 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 109
Score = 36.8 bits (74), Expect(3) = 4e-050
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +2
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 116 YGGKEESRKSNYTDMVN 166
Score = 35.9 bits (72), Expect(3) = 2e-031
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -2
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 105 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 16
Score = 24.9 bits (48), Expect(3) = 2e-031
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -2
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 156 SV*LDFLDSSFPP 118
>gb|AV917169.1|AV917169 AV917169 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8c03 5', mRNA sequence
Length = 503
Score = 91.3 bits (193), Expect(3) = 8e-045
Identities = 38/48 (79%), Positives = 42/48 (87%)
Frame = +2 / +1
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSS 397
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARF S
Sbjct: 124 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFRS 267
Score = 90.4 bits (191), Expect(3) = 8e-045
Identities = 36/56 (64%), Positives = 40/56 (71%)
Frame = +2 / +3
Query: 449 ELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
EL S GLS C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+Y +K
Sbjct: 264 ELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYPCIK 431
Score = 73.5 bits (154), Expect(3) = 1e-021
Identities = 29/45 (64%), Positives = 35/45 (77%)
Frame = -2 / -1
Query: 393 LNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMASREVSP 259
LNLAIS+ GPPIP PTSKTFIP + +C A+KC CRL+ S + SP
Sbjct: 263 LNLAISLNGPPIPQPTSKTFIPGFNSSCRARKCSCRLILSLKDSP 129
Score = 41.4 bits (84), Expect(3) = 1e-021
Identities = 21/43 (48%), Positives = 24/43 (55%)
Frame = -1 / -1
Query: 616 FQHTVDLTIDALCIWCMTCSLYCIGRIKGVIRYWHVHEVPLQE 488
F VDL I A I + C L I IK VIR WH+HEV L +
Sbjct: 431 FNTRVDLLIAANRIRRVACCLNGINSIKSVIREWHLHEVCLDK 303
Score = 36.8 bits (74), Expect(3) = 8e-045
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 4 YGGKEESRKSNYTDMVN 54
Score = 24.9 bits (48), Expect(3) = 1e-021
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -1
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 44 SV*LDFLDSSFPP 6
>gb|BE215659.3|BE215659 HV_CEb0007L14f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0007L14f, mRNA sequence
Length = 523
Score = 124 bits (266), Expect(3) = 1e-044
Identities = 51/67 (76%), Positives = 57/67 (85%)
Frame = +2 / +1
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYH 433
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL E SSTS+TGLNNNDY
Sbjct: 316 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREXCEISSTSVTGLNNNDYQ 495
Query: 434 ISRGKEL 454
I+RGKEL
Sbjct: 496 ITRGKEL 516
Score = 95.0 bits (201), Expect(3) = 3e-026
Identities = 39/66 (59%), Positives = 49/66 (74%)
Frame = -2 / -3
Query: 456 MSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMA 277
+SS PL +W*SLLF+PV +VEL S+ GPPIP PTSKTFIP + +C A+KC CRL+
Sbjct: 518 LSSFPLVIW*SLLFNPVTEVELISQXSLNGPPIPQPTSKTFIPGFNSSCRARKCSCRLIL 339
Query: 276 SREVSP 259
S + SP
Sbjct: 338 SLKDSP 321
Score = 56.5 bits (117), Expect(3) = 1e-044
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +1
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 91 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 189
Score = 36.8 bits (74), Expect(3) = 1e-044
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 196 YGGKEESRKSNYTDMVN 246
Score = 35.9 bits (72), Expect(3) = 3e-026
Identities = 18/30 (60%), Positives = 19/30 (63%)
Frame = -1 / -3
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDL 107
Y +Y STADLT SL I PP A APDL
Sbjct: 185 YFSYSSTADLTCSLSIFPPRPDARSRAPDL 96
Score = 24.9 bits (48), Expect(3) = 3e-026
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -3 / -3
Query: 248 SV*FDFLDSCLPP 210
SV* DFLDS PP
Sbjct: 236 SV*LDFLDSSFPP 198
>gb|BQ472834.1|BQ472834 HB01F10r BC Hordeum vulgare subsp. vulgare cDNA clone HB01F10
5-PRIME, mRNA sequence
Length = 404
Score = 154 bits (331), Expect = 4e-037
Identities = 63/84 (75%), Positives = 68/84 (80%)
Frame = +2 / +2
Query: 368 PLIEIARFSSTSITGLNNNDYHISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAY 547
PL EIARFSSTS+TGLNNNDY I+RGKEL S GLS C F+K DFM MP SDNTFDA Y
Sbjct: 2 PLREIARFSSTSVTGLNNNDYQITRGKELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVY 181
Query: 548 AIQATCHAPDAQGVYSEVYRVLKP 619
AI+ATCHAPD G Y E+YRVLKP
Sbjct: 182 AIEATCHAPDPVGCYKEIYRVLKP 253
>gb|BF257939.2|BF257939 HVSMEf0014E17f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0014E17f, mRNA sequence
Length = 571
Score = 139 bits (299), Expect = 1e-032
Identities = 56/121 (46%), Positives = 79/121 (65%)
Frame = +2 / +2
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG + REA + H+ +A L + G ++LDVGCG+GGP+ IA S + + G+ N+Y +
Sbjct: 134 PGRSHREATRVHEERVADLLEARPGKRLLDVGCGVGGPMRAIAAHSGSDVVGITINEYQV 313
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+R + AGL QC + G+FM MP+ D +FD AY+I+ATCHAP VY EV+RVLK
Sbjct: 314 NRARSHNRMAGLDNQCEVVCGNFMAMPVPDASFDGAYSIEATCHAPRLADVYGEVFRVLK 493
Query: 617 P 619
P
Sbjct: 494 P 496
>gb|CA028533.1|CA028533 HZ62F08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ62F08
5-PRIME, mRNA sequence
Length = 344
Score = 139 bits (299), Expect = 1e-032
Identities = 56/76 (73%), Positives = 61/76 (80%)
Frame = +2 / +1
Query: 392 SSTSITGLNNNDYHISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHA 571
SSTS+TGLNNNDY I+RGKEL S GLS C F+K DFM MP SDNTFDA YAI+ATCHA
Sbjct: 1 SSTSVTGLNNNDYQITRGKELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHA 180
Query: 572 PDAQGVYSEVYRVLKP 619
PD G Y E+YRVLKP
Sbjct: 181 PDPVGCYKEIYRVLKP 228
>gb|BQ468511.1|BQ468511 HM01H13T HM Hordeum vulgare subsp. vulgare cDNA clone HM01H13
5-PRIME, mRNA sequence
Length = 525
Score = 75.3 bits (158), Expect(3) = 5e-030
Identities = 30/38 (78%), Positives = 34/38 (89%)
Frame = +2 / +2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGG 367
+ GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGG
Sbjct: 410 WSGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGG 523
Score = 56.5 bits (117), Expect(3) = 5e-030
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +3 / +2
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+KSGAL LA GG I K+QVKSAVD+Y KYH
Sbjct: 185 MSKSGALDLASGLGGKIDKEQVKSAVDEYEKYH 283
Score = 36.8 bits (74), Expect(3) = 5e-030
Identities = 12/17 (70%), Positives = 17/17 (100%)
Frame = +1 / +2
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+ESRKSNYTD+++
Sbjct: 290 YGGKEESRKSNYTDMVN 340
>gb|BU975772.1|BU975772 HB32E04r BC Hordeum vulgare subsp. vulgare cDNA clone HB32E04
5-PRIME, mRNA sequence
Length = 521
Score = 128 bits (274), Expect = 3e-029
Identities = 51/70 (72%), Positives = 55/70 (78%)
Frame = +2 / +1
Query: 410 GLNNNDYHISRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGV 589
GLNNNDY I+RGKEL S GLS C F+K DFM MP SDNTFDA YAI+ATCHAPD G
Sbjct: 13 GLNNNDYQITRGKELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGC 192
Query: 590 YSEVYRVLKP 619
Y E+YRVLKP
Sbjct: 193 YKEIYRVLKP 222
>gb|BF619597.3|BF619597 HVSMEc0004K03f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0004K03f, mRNA sequence
Length = 588
Score = 115 bits (246), Expect = 2e-025
Identities = 46/91 (50%), Positives = 60/91 (65%)
Frame = +2 / +2
Query: 347 VGCGIGGPLIEIARFSSTSITGLNNNDYHISRGKELISSAGLSEQCCFLKGDFMDMPISD 526
VGCG+GGP+ IA S + G+ N+Y ++R + A L QC + G+FM MP D
Sbjct: 2 VGCGVGGPMRAIAAHSGAHVVGITINEYQVNRARAHNRKARLDAQCEVVCGNFMAMPFDD 181
Query: 527 NTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
+FD AY+I+ATCHAP Q VY+EVYRVLKP
Sbjct: 182 ASFDGAYSIEATCHAPRLQDVYAEVYRVLKP 274
>gb|BF266844.2|BF266844 HV_CEa0016D13f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0016D13f, mRNA sequence
Length = 571
Score = 110 bits (235), Expect = 7e-024
Identities = 45/121 (37%), Positives = 68/121 (56%)
Frame = +2 / +3
Query: 257 PGETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHI 436
PG + R+A + H+ + L + G +VLDVGCG+GGP+ I S + G N Y +
Sbjct: 102 PGRSHRDATRVHEERVXDLLAARPGHRVLDVGCGVGGPMRAIGAHSGAHVVGFTINKYQV 281
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+R + A + C G+FM MP + F AY+I+ TCH P + ++++VYRVLK
Sbjct: 282 NRARAHNRKASVDTHCKGGCGNFMAMPFDNTFFYGAYSIEGTCHTPLLEDLFTQVYRVLK 461
Query: 617 P 619
P
Sbjct: 462 P 464
>gb|BU971669.1|BU971669 HB19E23r BC Hordeum vulgare subsp. vulgare cDNA clone HB19E23
5-PRIME, mRNA sequence
Length = 520
Score = 107 bits (229), Expect = 5e-023
Identities = 43/61 (70%), Positives = 47/61 (77%)
Frame = +2 / +2
Query: 437 SRGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLK 616
+RGKEL S GLS C F+K DFM MP SDNTFDA YAI+ATCHAPD G Y E+YRVLK
Sbjct: 5 TRGKELNRSVGLSGTCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVLK 184
Query: 617 P 619
P
Sbjct: 185 P 187
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 308,842,757
Number of Sequences: 312970
Number of extensions: 4777439
Number of successful extensions: 51005
Number of sequences better than 1.0e-020: 27
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6532173186
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)