TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3748389.2.1
(663 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG300526.1|BG300526 HVSMEb0017F22f Hordeum vulgare seedl... 144 3e-045
gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedl... 96 4e-029
gb|BM101456.2|BM101456 EBpi01_SQ003_O13_R pistil, 1 DPA, no... 90 5e-028
gb|BF623637.2|BF623637 HVSMEa0003E04f Hordeum vulgare seedl... 95 2e-026
gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedl... 84 9e-026
gb|CA025121.1|CA025121 HZ51E09r HZ Hordeum vulgare subsp. v... 90 1e-024
gb|BU998386.1|BU998386 HI10O06r HI Hordeum vulgare subsp. v... 79 4e-023
gb|BM443110.2|BM443110 EBro02_SQ002_J16_R root, 3 week, hyd... 83 5e-023
emb|X15694.1|HVBRNZ1H Barley bronze 1 homologue for UDPgluc... 68 1e-021
gb|CA028126.1|CA028126 HZ61B05r HZ Hordeum vulgare subsp. v... 81 1e-021
>gb|BG300526.1|BG300526 HVSMEb0017F22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0017F22f, mRNA sequence
Length = 949
Score = 144 bits (309), Expect(3) = 3e-045
Identities = 57/99 (57%), Positives = 74/99 (74%)
Frame = +3 / -2
Query: 168 EERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLPLMFDQGLN 347
++R++GRG VCR W PQ + LAHESVGGFLTHAGW SI +GL GV + LLP++F+QGL
Sbjct: 525 KKRLSGRGFVCRSWEPQDKFLAHESVGGFLTHAGWKSIPQGLGHGVRLGLLPMVFEQGLK 346
Query: 348 ARLLMDKKIGIEVERNEDDGTFAPKDIADALRTAMAENQ 464
AR ++ KKIG++V R+E DG F IA ALR M E++
Sbjct: 345 ARNIVGKKIGVKVARDEQDGLFVAGKIAAALRRVMVEDE 229
Score = 100 bits (214), Expect(2) = 5e-025
Identities = 51/108 (47%), Positives = 63/108 (58%)
Frame = -3 / +2
Query: 457 SAMAVLRASAMSLGANVPSSSFLSTSIPIFLSIRSLALRP*SNISGNSTIVTPRERPSAM 278
S M +L+A+A+ N PS S +T PIFL LALRP SN G+ +TP RP +
Sbjct: 236 STMTLLKAAAILPATNNPSCSSRATLTPIFLPTMFLALRPCSNTIGSRPSLTPCPRPWGI 415
Query: 277 EFQPAWVRNPPTDS*ANSLG*GTQPRQTNPRPLTRSSKPSGNPPVPCP 134
+FQPA VRNPPTDS A +L G+Q R TNPRP + K P P
Sbjct: 416 DFQPACVRNPPTDSWARNLSCGSQLRHTNPRPESLFFKAPWQTPRKMP 559
Score = 95.0 bits (201), Expect(3) = 3e-024
Identities = 42/93 (45%), Positives = 57/93 (61%)
Frame = -2 / +1
Query: 461 VLRHGRPQSIRDVFGRERAVVFVPLHLNPDLLIHKKPGVEALIEHQRQQHHRDAPREAFG 282
V H PQS R+ G +++V+ VP HLNPD H PG+EAL++H R+ + +A G
Sbjct: 232 VFHHDPPQSRRNFAGHKQSVLLVPRHLNPDFFAHYVPGLEALLKHHRK*TQPHSVPQALG 411
Query: 281 DGVPAGVGQKPTNRFMSQQPGLRHPAAADQSPA 183
D P GV Q+P N FM Q+ LR P +A +SPA
Sbjct: 412 DRFPTGVRQEPPN*FMGQKLILRLPTSAHKSPA 510
Score = 52.4 bits (108), Expect(3) = 3e-045
Identities = 19/37 (51%), Positives = 30/37 (81%)
Frame = +3 / -2
Query: 468 GTRVKELAEVFGNDEANDQCLRDFLRYLSEYIRQHQG 578
G +VKELA+VFG+D+ NDQC++ F ++L I+++QG
Sbjct: 216 GAKVKELAKVFGDDKVNDQCVKKFFKHL*GRIKKNQG 106
Score = 33.1 bits (66), Expect(2) = 5e-025
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = -2 / +1
Query: 581 LTLMLPDVF*QIPEEISQALIIGLVISKHLGQFLDPSP 468
L+L+ D Q+ E++ ALII LV+ +H GQ L P
Sbjct: 103 LSLVFFDAALQVLEKLFHALIIHLVVPEHFGQLLYLGP 216
Score = 29.5 bits (58), Expect(3) = 3e-024
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = -3 / +2
Query: 526 H*SLASSFPNTSASSLTLVPS 464
H*S S PNT ASS TL P+
Sbjct: 158 H*SFTLSSPNTLASSFTLAPN 220
Score = 24.4 bits (47), Expect(3) = 3e-024
Identities = 13/35 (37%), Positives = 14/35 (40%)
Frame = -3 / +3
Query: 172 SSKPSGNPPVPCPGAASAGGLNAQRNGRPEASKPS 68
S KP G PP CP G N R +PS
Sbjct: 522 SLKPLGKPPGRCPRFLVNIGALNSPNKRGSPGRPS 626
Score = 23.5 bits (45), Expect(3) = 3e-045
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +3 / -1
Query: 135 GQGTGGLPEGFEERVNGRGL 194
G GGLP GF+E+ G+
Sbjct: 559 GHLPGGLPRGFKEKAFRPGI 500
>gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001A18f, mRNA sequence
Length = 612
Score = 95.9 bits (203), Expect(2) = 4e-029
Identities = 38/69 (55%), Positives = 47/69 (68%)
Frame = +3 / +3
Query: 159 EGFEERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLPLMFDQ 338
EGFEERV RGL+ RGW PQ +L+H +VGGFLTH GWN+ E +S GV + P DQ
Sbjct: 207 EGFEERVKDRGLLVRGWAPQVTILSHPAVGGFLTHCGWNATLEAISHGVPALTWPNFADQ 386
Query: 339 GLNARLLMD 365
+ RLL+D
Sbjct: 387 FCSERLLVD 413
Score = 51.9 bits (107), Expect(2) = 4e-029
Identities = 21/37 (56%), Positives = 28/37 (75%)
Frame = +3 / +3
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFLWAFR 113
SV+Y +FGS AKL + Q+ +A GLEASG PF+WA +
Sbjct: 54 SVLYISFGSIAKLPAKQVAELARGLEASGRPFVWAIK 164
>gb|BM101456.2|BM101456 EBpi01_SQ003_O13_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ003_O13 5', mRNA sequence
Length = 355
Score = 90.0 bits (190), Expect(2) = 5e-028
Identities = 36/59 (61%), Positives = 44/59 (74%)
Frame = +3 / +1
Query: 159 EGFEERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLPLMFD 335
+GF ERV GRG+V GWVPQ R+LAH +VGGFLTHAGW+S+ E G +V+LPL D
Sbjct: 178 DGFAERVAGRGVVRAGWVPQVRVLAHGAVGGFLTHAGWSSLMESFLFGHPLVMLPLFAD 354
Score = 53.8 bits (111), Expect(2) = 5e-028
Identities = 22/37 (59%), Positives = 27/37 (72%)
Frame = +3 / +1
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFLWAFR 113
SV+Y AFGSEA LT+ ++ +A GLE SG FLWA R
Sbjct: 40 SVLYVAFGSEAPLTAENIRELAAGLELSGARFLWALR 150
>gb|BF623637.2|BF623637 HVSMEa0003E04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0003E04f, mRNA sequence
Length = 485
Score = 94.5 bits (200), Expect(2) = 2e-026
Identities = 34/71 (47%), Positives = 49/71 (69%)
Frame = +3 / +1
Query: 153 LPEGFEERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLPLMF 332
L +GFE RV RG++ RGW PQ +L H+++GGF+TH GWNS EG+ GV M+ P
Sbjct: 268 LADGFEARVKDRGMILRGWAPQVMILWHQAIGGFVTHCGWNSTIEGICAGVPMITWPHFS 447
Query: 333 DQGLNARLLMD 365
+Q +N +L++D
Sbjct: 448 EQFVNEKLVVD 480
Score = 44.1 bits (90), Expect(2) = 2e-026
Identities = 18/37 (48%), Positives = 24/37 (64%)
Frame = +3 / +1
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFLWAFR 113
SV++ +FGS A T QL + LGLEAS PF+W +
Sbjct: 124 SVIFVSFGSLACTTPQQLVELGLGLEASQKPFIWVIK 234
>gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0017B13f, mRNA sequence
Length = 875
Score = 84.5 bits (178), Expect(2) = 9e-026
Identities = 34/65 (52%), Positives = 42/65 (64%)
Frame = +3 / +3
Query: 153 LPEGFEERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLPLMF 332
LP GF R RGLV + W PQ +LAH +VGGF+TH GWNS+ E + GV M+ PL
Sbjct: 198 LPPGFLARTQERGLVVKTWAPQRDVLAHGAVGGFVTHCGWNSVLEAVMAGVPMLAWPLYA 377
Query: 333 DQGLN 347
+Q LN
Sbjct: 378 EQRLN 392
Score = 51.9 bits (107), Expect(2) = 9e-026
Identities = 24/53 (45%), Positives = 32/53 (60%)
Frame = +3 / +3
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFLWAFRPPADAAPGQGTGGLPE 161
SVV+ FGS ++AQ++ IA+GLEASG FLW R P + P + PE
Sbjct: 21 SVVFLCFGSLGVFSAAQIREIAVGLEASGNRFLWVVRNPPNEDPARRFEEPPE 179
>gb|CA025121.1|CA025121 HZ51E09r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ51E09
5-PRIME, mRNA sequence
Length = 526
Score = 90.0 bits (190), Expect(3) = 1e-024
Identities = 38/67 (56%), Positives = 45/67 (67%)
Frame = +3 / +3
Query: 156 PEGFEERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLPLMFD 335
P GFEER GRG+V GWV Q +L H SVG F+ HAG +S+ EGL G +VLLP+ D
Sbjct: 168 PPGFEERTKGRGVVHTGWVQQQHILRHRSVGCFVNHAGLSSVVEGLVAGCRLVLLPMKGD 347
Query: 336 QGLNARL 356
Q LNA L
Sbjct: 348 QYLNAAL 368
Score = 36.3 bits (73), Expect(3) = 1e-024
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = +3 / +3
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFL 101
+VV+A+FGSE L + + LGLEA+ PFL
Sbjct: 12 AVVFASFGSETFLPADAATELLLGLEATNRPFL 110
Score = 24.0 bits (46), Expect(3) = 1e-024
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +1 / +1
Query: 373 SGLRWRGTKTTARSRPKTSRML*GRPWR 456
SG RWRG T S T R WR
Sbjct: 388 SGRRWRGATATGGSGAGT*TTPWKRQWR 471
>gb|BU998386.1|BU998386 HI10O06r HI Hordeum vulgare subsp. vulgare cDNA clone HI10O06
5-PRIME, mRNA sequence
Length = 635
Score = 79.0 bits (166), Expect(2) = 4e-023
Identities = 28/58 (48%), Positives = 38/58 (65%)
Frame = +3 / +3
Query: 153 LPEGFEERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLPL 326
+PEGF ER RGLV + W PQ +L H + G F+TH GWNS EG++ G+ ++ PL
Sbjct: 453 MPEGFLERTKDRGLVVKSWAPQVDVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPL 626
Score = 48.7 bits (100), Expect(2) = 4e-023
Identities = 21/39 (53%), Positives = 26/39 (66%)
Frame = +3 / +3
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFLWAFRPP 119
SVV+ +FGS +S QL+ IALGL+ SG FLW R P
Sbjct: 279 SVVFLSFGSLGTFSSKQLEEIALGLDKSGERFLWVVRSP 395
>gb|BM443110.2|BM443110 EBro02_SQ002_J16_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ002_J16 5', mRNA sequence
Length = 427
Score = 83.1 bits (175), Expect(2) = 5e-023
Identities = 29/57 (50%), Positives = 39/57 (68%)
Frame = +3 / +3
Query: 153 LPEGFEERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLP 323
L +GFE RV RG++ R W PQ +L H+++GGF+TH GWNS EG+ GV M+ P
Sbjct: 255 LADGFEARVKDRGMILRSWAPQVMILWHQAIGGFVTHCGWNSTIEGICAGVPMITWP 425
Score = 44.1 bits (90), Expect(2) = 5e-023
Identities = 18/37 (48%), Positives = 24/37 (64%)
Frame = +3 / +3
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFLWAFR 113
SV++ +FGS A T QL + LGLEAS PF+W +
Sbjct: 111 SVIFVSFGSLACTTPQQLVELGLGLEASQKPFIWVIK 221
>emb|X15694.1|HVBRNZ1H Barley bronze 1 homologue for UDPglucose flavonol 3,0 glucosyl
transferase
Length = 2326
Score = 68.0 bits (142), Expect(2) = 1e-021
Identities = 26/49 (53%), Positives = 32/49 (65%)
Frame = +3 / +2
Query: 207 WVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGVTMVLLPLMFDQGLNAR 353
W PQ +L H +VG F+THAGW S+ EG+S GV M P DQ +NAR
Sbjct: 1664 WAPQVGVLRHAAVGAFVTHAGWASVMEGVSSGVPMACRPFFGDQTMNAR 1810
Score = 54.7 bits (113), Expect(2) = 1e-021
Identities = 23/44 (52%), Positives = 28/44 (63%)
Frame = +3 / +2
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFLWAFRPPADAAP 134
SV Y +FG+ A +LQ +A GLEASG PFLW+ R AAP
Sbjct: 1493 SVAYVSFGTNATARPDELQELAAGLEASGAPFLWSLRGVVAAAP 1624
>gb|CA028126.1|CA028126 HZ61B05r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ61B05
5-PRIME, mRNA sequence
Length = 565
Score = 80.8 bits (170), Expect(2) = 1e-021
Identities = 29/49 (59%), Positives = 36/49 (73%)
Frame = +3 / +3
Query: 159 EGFEERVNGRGLVCRGWVPQPRLLAHESVGGFLTHAGWNSIAEGLSRGV 305
E E R +GRGLV RGW PQ +L+H SVGGF+TH GWNS+ E ++ GV
Sbjct: 411 ENLEARTSGRGLVVRGWAPQLAVLSHRSVGGFVTHCGWNSVLESIAHGV 557
Score = 41.8 bits (85), Expect(2) = 1e-021
Identities = 20/44 (45%), Positives = 24/44 (54%)
Frame = +3 / +3
Query: 3 SVVYAAFGSEAKLTSAQLQTIALGLEASGLPFLWAFRPPADAAP 134
SVVY +FGS A+ Q I GLE G PFLW + A+P
Sbjct: 261 SVVYVSFGSVARKLPKQQFEIGHGLEHCGRPFLWVVKESELASP 392
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 345,442,673
Number of Sequences: 312970
Number of extensions: 7370140
Number of successful extensions: 144474
Number of sequences better than 1.0e-020: 11
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7168423821
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)