TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3742446.2.1
(598 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU976210.1|BU976210 HA03F10r HA Hordeum vulgare subsp. v... 288 9e-083
gb|AV916601.1|AV916601 AV916601 K. Sato unpublished cDNA li... 249 4e-069
gb|BU970797.1|BU970797 HB15L12r BC Hordeum vulgare subsp. v... 253 6e-067
gb|BJ465257.1|BJ465257 BJ465257 K. Sato unpublished cDNA li... 250 4e-066
gb|BQ764926.1|BQ764926 EBca01_SQ005_E07_R carpel, pre-anthe... 238 2e-062
gb|AV916609.1|AV916609 AV916609 K. Sato unpublished cDNA li... 229 8e-061
gb|BJ464778.1|BJ464778 BJ464778 K. Sato unpublished cDNA li... 226 6e-059
gb|BU997130.1|BU997130 HI07A02r HI Hordeum vulgare subsp. v... 220 7e-059
gb|BJ463364.1|BJ463364 BJ463364 K. Sato unpublished cDNA li... 226 8e-059
gb|AV917009.1|AV917009 AV917009 K. Sato unpublished cDNA li... 220 5e-057
gb|AV917011.1|AV917011 AV917011 K. Sato unpublished cDNA li... 218 2e-056
gb|BU988606.1|BU988606 HF18C11r HF Hordeum vulgare subsp. v... 202 7e-055
gb|BQ468641.1|BQ468641 HM01N16T HM Hordeum vulgare subsp. v... 204 4e-054
gb|BQ464272.1|BQ464272 HF01M24T HF Hordeum vulgare subsp. v... 197 4e-050
gb|CA026968.1|CA026968 HZ57H22r HZ Hordeum vulgare subsp. v... 180 2e-048
gb|CK122337.1|CK122337 BES1824102p02 BES1824 Hordeum vulgar... 131 1e-046
gb|BI948619.1|BI948619 HVSMEl0010E10f Hordeum vulgare spike... 185 2e-046
gb|BU978697.1|BU978697 HA14A09r HA Hordeum vulgare subsp. v... 177 5e-046
gb|BU994352.1|BU994352 HM06L21r HM Hordeum vulgare subsp. v... 181 4e-045
gb|AV916387.1|AV916387 AV916387 K. Sato unpublished cDNA li... 168 6e-045
gb|BJ462621.1|BJ462621 BJ462621 K. Sato unpublished cDNA li... 168 6e-045
gb|AV913558.1|AV913558 AV913558 K. Sato unpublished cDNA li... 178 2e-044
gb|BE601754.2|BE601754 HVSMEh0099G19f Hordeum vulgare 5-45 ... 158 8e-044
gb|AV924652.1|AV924652 AV924652 K. Sato unpublished cDNA li... 105 5e-042
gb|BQ472678.1|BQ472678 HB01N04r BC Hordeum vulgare subsp. v... 116 5e-042
gb|BJ462547.1|BJ462547 BJ462547 K. Sato unpublished cDNA li... 170 5e-042
gb|AV932210.1|AV932210 AV932210 K. Sato unpublished cDNA li... 118 3e-041
gb|BJ547617.1|BJ547617 BJ547617 K. Sato unpublished cDNA li... 118 3e-041
gb|BJ547606.1|BJ547606 BJ547606 K. Sato unpublished cDNA li... 118 3e-041
gb|BG367505.2|BG367505 HVSMEi0012I04f Hordeum vulgare 20 DA... 167 4e-041
gb|BI947961.1|BI947961 HVSMEl0007J23f Hordeum vulgare spike... 112 6e-040
gb|BI952746.1|BI952746 HVSMEm0007J23f Hordeum vulgare green... 112 6e-040
gb|BQ464194.1|BQ464194 HF01J17T HF Hordeum vulgare subsp. v... 108 1e-039
gb|AV915505.1|AV915505 AV915505 K. Sato unpublished cDNA li... 161 2e-039
gb|BU986160.1|BU986160 HF09N16r HF Hordeum vulgare subsp. v... 160 4e-039
gb|BU986567.1|BU986567 HF12E04r HF Hordeum vulgare subsp. v... 157 5e-038
gb|BE231065.1|BE231065 HVSMEg0016D19f Hordeum vulgare pre-a... 156 8e-038
gb|BU998469.1|BU998469 HI11C15r HI Hordeum vulgare subsp. v... 110 1e-037
gb|BF261097.2|BF261097 HVSMEf0023M01f Hordeum vulgare seedl... 155 2e-037
gb|BU984656.1|BU984656 HF04J11r HF Hordeum vulgare subsp. v... 100 4e-037
gb|BJ547686.1|BJ547686 BJ547686 K. Sato unpublished cDNA li... 118 1e-034
gb|BF253874.2|BF253874 HVSMEf0002F19f Hordeum vulgare seedl... 145 2e-034
gb|AV912902.1|AV912902 AV912902 K. Sato unpublished cDNA li... 68 7e-034
gb|BQ472621.1|BQ472621 HB01P17r BC Hordeum vulgare subsp. v... 89 7e-034
gb|BU997151.1|BU997151 HI07B06r HI Hordeum vulgare subsp. v... 135 1e-033
gb|BQ471321.1|BQ471321 HV02B13r HV Hordeum vulgare subsp. v... 115 2e-033
gb|BM441924.2|BM441924 EBed07_SQ001_O24_R endosperm, 28 DPA... 141 3e-033
gb|CA026718.1|CA026718 HZ56L20r HZ Hordeum vulgare subsp. v... 125 3e-032
gb|AV913799.1|AV913799 AV913799 K. Sato unpublished cDNA li... 125 4e-032
gb|AV916990.1|AV916990 AV916990 K. Sato unpublished cDNA li... 125 4e-032
gb|AV916192.1|AV916192 AV916192 K. Sato unpublished cDNA li... 125 4e-032
gb|AV912838.1|AV912838 AV912838 K. Sato unpublished cDNA li... 134 4e-031
gb|BJ464115.1|BJ464115 BJ464115 K. Sato unpublished cDNA li... 131 4e-030
gb|CA008162.1|CA008162 HU10B07r HU Hordeum vulgare subsp. v... 75 8e-030
gb|BG301295.1|BG301295 HVSMEb0020I16f Hordeum vulgare seedl... 108 2e-029
gb|BI955975.1|BI955975 HVSMEm0025E04f Hordeum vulgare green... 90 4e-029
gb|BJ451325.1|BJ451325 BJ451325 K. Sato unpublished cDNA li... 73 2e-028
gb|BF263416.3|BF263416 HV_CEa0006L01f Hordeum vulgare seedl... 93 3e-028
gb|BG368358.1|BG368358 HVSMEi0017P04f Hordeum vulgare 20 DA... 123 6e-028
gb|AV914116.1|AV914116 AV914116 K. Sato unpublished cDNA li... 123 6e-028
gb|BG300683.1|BG300683 HVSMEb0017P19f Hordeum vulgare seedl... 72 7e-027
gb|BI951286.1|BI951286 HVSMEl0025F23f Hordeum vulgare spike... 79 2e-026
gb|BU995874.1|BU995874 HM11K09r HM Hordeum vulgare subsp. v... 70 3e-026
gb|BG417583.2|BG417583 HVSMEk0018E23f Hordeum vulgare testa... 118 3e-026
gb|BQ766937.1|BQ766937 EBro08_SQ007_D17_R root, 3 week, dro... 118 3e-026
gb|BI947104.1|BI947104 HVSMEl0003L04f Hordeum vulgare spike... 70 2e-025
gb|BE194623.2|BE194623 HVSMEh0086E10f Hordeum vulgare 5-45 ... 81 1e-024
gb|BJ452216.1|BJ452216 BJ452216 K. Sato unpublished cDNA li... 112 1e-024
gb|BF257586.2|BF257586 HVSMEf0013E13f Hordeum vulgare seedl... 96 2e-024
gb|BJ465335.1|BJ465335 BJ465335 K. Sato unpublished cDNA li... 110 6e-024
gb|BU986367.1|BU986367 HF11J16r HF Hordeum vulgare subsp. v... 110 6e-024
gb|BF620280.1|BF620280 HVSMEc0019E21f Hordeum vulgare seedl... 82 8e-023
gb|BJ462504.1|BJ462504 BJ462504 K. Sato unpublished cDNA li... 92 4e-022
gb|BM373123.2|BM373123 EBma04_SQ003_D03_R maternal, 10 DPA,... 88 5e-022
gb|AV914656.1|AV914656 AV914656 K. Sato unpublished cDNA li... 104 5e-022
gb|BQ758950.1|BQ758950 EBma07_SQ003_G09_R maternal, 21 DPA,... 102 1e-021
gb|BF630973.2|BF630973 HVSMEb0014J12f Hordeum vulgare seedl... 79 5e-021
gb|AV913514.1|AV913514 AV913514 K. Sato unpublished cDNA li... 68 6e-021
>gb|BU976210.1|BU976210 HA03F10r HA Hordeum vulgare subsp. vulgare cDNA clone HA03F10
5-PRIME, mRNA sequence
Length = 571
Score = 288 bits (623), Expect(2) = 9e-083
Identities = 114/166 (68%), Positives = 124/166 (74%)
Frame = +2 / +2
Query: 59 LGWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAG 238
LGWLSPKIV AFADYAEFCF FGDRVKNWFTFNEPR VAALGYDNG HAPGRCS CPAG
Sbjct: 74 LGWLSPKIVGAFADYAEFCFKVFGDRVKNWFTFNEPRVVAALGYDNGFHAPGRCSKCPAG 253
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
G+S TEPY +RYR+KYQ HQKG+IGILLDFVWYEP SDSN
Sbjct: 254 GDSRTEPYIVTHNIILSHAAAVQRYREKYQPHQKGRIGILLDFVWYEPHSDSNADQAAAQ 433
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSI 556
FH+GWFLDPI +GRYP SM +I +RLP FS +E+RM KGSI
Sbjct: 434 RARDFHIGWFLDPITNGRYPSSMLKIVGNRLPGFSADESRMVKGSI 571
Score = 198 bits (426), Expect(2) = 1e-052
Identities = 98/163 (60%), Positives = 114/163 (69%)
Frame = -1 / -1
Query: 550 AFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLVRIAVAERFVP 371
AF H G AE R PV NL HR + ++ +GV+EP+ VEVP L SL+ IAVA RFVP
Sbjct: 565 AFHHSGLIGAEARQPVPDNLEHRRWIATIGDGVEEPSNVEVPRPLRGSLIGIAVAVRFVP 386
Query: 370 HEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPGTPSRCVQAIV 191
HEIQEN N PLLV LIL+ VS R MRKD++V D VR R GVAS A G P R ++AIV
Sbjct: 385 HEIQENPNPPLLVWLILLPVSLHRCGMRKDDIVRDNVRLRPGVASCRALGAPPRRMEAIV 206
Query: 190 VAQSSDAPRLVEGKPVLHPVSERVEAELGVVCKRLHNLWAKPA 62
V QS D P LVEG+PVLHPV E +EAELG+V K H+LWA PA
Sbjct: 205 VPQSGDDPWLVEGEPVLHPVPEHLEAELGIVRKCAHDLWA*PA 77
Score = 197 bits (425), Expect(2) = 5e-052
Identities = 98/164 (59%), Positives = 104/164 (63%)
Frame = +3 / +3
Query: 57 TWAGLAQRLWRRLQTTPSSASTRSETG*RTGLPSTSRGASLLWATTMACTHREGVPGAPP 236
T AG AQR W L+T PSSAS S TG*RTG PSTS+G+S LW TTMA G P A
Sbjct: 72 T*AG*AQRSWAHLRTMPSSASRCSGTG*RTGSPSTSQGSSPLWGTTMASMRLGGAPSALQ 251
Query: 237 EATPPRNRTLSHTISSFLMQLRPGDTATSISFTRRGRLEFSWISCGTNLSATAMRTRLQH 416
EATP R+RTLS TISSF M R DT SIS TRRG L FSWISCGTNL+ATAM +L
Sbjct: 252 EATPGRSRTLSRTISSFRMPQRCNDTGRSISHTRRGGLGFSWISCGTNLTATAMPIKLPR 431
Query: 417 SEPGTST*AGSLTPLYMDGTRTRCKRLPKTGYRCSAMKKPGWRK 548
GTST GS TP M RC RL TG R SA P W K
Sbjct: 432 RGRGTSTLDGSSTPSPMVAIHRRCSRLSGTGCRASAPMSPEW*K 563
Score = 186 bits (400), Expect = 1e-046
Identities = 77/141 (54%), Positives = 98/141 (69%)
Frame = +1 / +1
Query: 133 QGEELVYLQRAEVRRCSGLRQWLARTGKVFRVPRRRQLHHGTVPCRTPSHPFSCSCGQAI 312
QGEELV+LQRA+ RR SG+RQWL +V +VP RR+L G V C HPF+C G I
Sbjct: 148 QGEELVHLQRAKGRRRSGVRQWLPCAWEVLQVPCRRRLQDGAVHCHAQYHPFACRSGATI 327
Query: 313 PRQVSASPEGEDWNSPGFRVVRTFQRQQCGPGCSTASQGLPPRLVP*PHCTWTVPVLDAR 492
P +VSA+PEGEDW+S GFRVVRT QRQQC C +GLP +VP PH W++ ++DA+
Sbjct: 328 PGEVSATPEGEDWDSLGFRVVRTSQRQQCRSSCRAEGEGLPHWMVPRPHHQWSLSIVDAQ 507
Query: 493 DCQRQATVVQR*RSQDGERLY 555
DC+ Q +QR Q+GERL+
Sbjct: 508 DCREQVAGLQRR*VQNGERLH 570
Score = 144 bits (310), Expect(3) = 3e-060
Identities = 64/77 (83%), Positives = 65/77 (84%)
Frame = -2 / -2
Query: 288 ERMRWCATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQN 109
ERM C T YGSV+E PPAGH EHLPGA KPLS PRAAT LGSLKVNQFFTLSPN KQN
Sbjct: 303 ERMILCVTMYGSVLESPPAGHLEHLPGAWKPLSYPRAATTLGSLKVNQFFTLSPNTLKQN 124
Query: 108 SA*SANASTIFGLSQPR 58
SA*SANA TIFGLSQPR
Sbjct: 123 SA*SANAPTIFGLSQPR 73
Score = 127 bits (272), Expect(2) = 4e-031
Identities = 70/156 (44%), Positives = 81/156 (51%)
Frame = -3 / -3
Query: 524 R*TTVACLWQSLASSTGTVHVQWGQGTSLGGSPWLAVLQPGPHCCR*KVRTTRNPGEFQS 345
R*+ C QS AS+ + H WG+GT GSP + Q HCCR +VRTTRNP E QS
Sbjct: 539 R*SPATCSRQS*ASTMDSDHW*WGRGTIQCGSPSPSARQLDRHCCRCEVRTTRNPRESQS 360
Query: 344 SPSGEADTCRGIAWPQLHEKG*DGVRQGTVPWWSCLRRGTRNTFPVRASHCRSPEQXXXX 165
SPSG ADT GI P H KG* Q T P WS L +GT +T SHCR+PE+
Sbjct: 359 SPSGVADTSPGIVAPLRHAKG*YCA*QCTAPSWSRLLQGTWSTSQAHGSHCRTPERRRPL 180
Query: 164 XXXXXXXXSPCXXXXXXXXXXXLQTPPQSLG*ASPG 57
SPC Q P+SLG AS G
Sbjct: 179 AR*R*TSSSPCPRTP*SRTRHSPQMRPRSLGLASLG 72
Score = 92.7 bits (196), Expect(3) = 3e-060
Identities = 39/51 (76%), Positives = 41/51 (80%)
Frame = -2 / -2
Query: 486 IEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPFW 334
I+ G RP MGSRN P WKSLA CAAA SALLSL GSYHTKS+RIPI PFW
Sbjct: 501 IDDG*RPLVMGSRNHPMWKSLALCAAA*SALLSL*GSYHTKSKRIPILPFW 349
Score = 38.6 bits (78), Expect(2) = 9e-083
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +3 / +2
Query: 15 NLYHYDLPLALHEQTWAGLAQRL 83
NLYHYDLPLALH+Q L+ ++
Sbjct: 29 NLYHYDLPLALHQQYLGWLSPKI 97
Score = 32.7 bits (65), Expect(3) = 3e-060
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = -1 / -2
Query: 55 CSWSANGRS*W*RF 14
C WSA+GRS*W*RF
Sbjct: 69 CWWSASGRS*W*RF 28
Score = 28.6 bits (56), Expect(2) = 1e-052
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -2 / -3
Query: 75 GLSQPRSVHGVPMGGHNGRDLST 7
GL+ + G P GGH+GR L T
Sbjct: 89 GLASLGTAGGAPAGGHSGRGLHT 21
Score = 26.7 bits (52), Expect(2) = 4e-031
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -3 / -1
Query: 56 LFMECQWEVIMVEICRRG 3
L +E Q EVI+VE+C RG
Sbjct: 70 LLVERQREVIVVEVCIRG 17
Score = 26.7 bits (52), Expect(2) = 5e-052
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +1 / +3
Query: 4 PRRQISTIMTSHWHS 48
PR Q ST MTS W S
Sbjct: 18 PRMQTSTTMTSRWRS 62
>gb|AV916601.1|AV916601 AV916601 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17p13 5', mRNA sequence
Length = 640
Score = 249 bits (539), Expect(2) = 4e-069
Identities = 96/175 (54%), Positives = 116/175 (66%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 113 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 292
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 293 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQR 472
Query: 422 XXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
FH+GWF+DP+++G YP MQ++ K+RLP F+ EA+M GS DY+GIN YT+
Sbjct: 473 ARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAEAKMVMGSADYIGINQYTA 637
Score = 121 bits (259), Expect = 3e-027
Identities = 67/175 (38%), Positives = 99/175 (56%)
Frame = -1 / -1
Query: 580 VVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLV 401
V++D NIV A H F +E P+F +LH + V +Y V +PA +EVPGSL C LV
Sbjct: 631 VLIDPNIVRGAHDHFSFSRSELGQPLFHKVLHYLWVMPVYQRVDKPANMEVPGSLSCCLV 452
Query: 400 RIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPG 221
V + VP EIQ +NLPLL LIL VS + +++V + VRF V +G A
Sbjct: 451 FS*VGKSLVPVEIQNYTNLPLLSCLILGPVSCNCITVC*EKIVSNNVRFCC*VPTGSAFA 272
Query: 220 TPSRCVQAIVVAQSSDAPRLVEGKPVLHPVSERVEAELGVVCKRLHNLWAKPAQV 56
P R ++ VV + + P +E KPVL+ +++ ++ ++ +VCK H L PA +
Sbjct: 271 APLRRIRPYVVTKKRNYPWFIECKPVLYTIAKGLKTKVSIVCK*SHRLCI*PASI 107
Score = 90.0 bits (190), Expect(3) = 6e-035
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -2
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 321 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 142
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 141 SPTVFAFNQP 112
Score = 73.0 bits (153), Expect(3) = 6e-035
Identities = 36/79 (45%), Positives = 41/79 (51%)
Frame = -2 / -2
Query: 573 LMPT*SIEPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCAAAWSAL 394
L+P *S EP SCI G P GS NQP WKSLA AAAWS++
Sbjct: 624 LIPI*SAEPMTILASAGVNLGNLSFTRSCIICG*CPFISGSTNQPTWKSLAL*AAAWSSV 445
Query: 393 LSLKGSYHTKSRRIPIFPF 337
++ SY KSR IP FPF
Sbjct: 444 ELVRASYQLKSRTIPTFPF 388
Score = 31.8 bits (63), Expect(2) = 4e-069
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +2
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 65 NLYHYDLPLALEKK 106
Score = 22.1 bits (42), Expect(3) = 6e-035
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -2
Query: 46 SANGRS*W*RFV 11
SA GRS*W*R +
Sbjct: 96 SARGRS*W*RLM 61
>gb|BU970797.1|BU970797 HB15L12r BC Hordeum vulgare subsp. vulgare cDNA clone HB15L12
5-PRIME, mRNA sequence
Length = 587
Score = 253 bits (547), Expect = 6e-067
Identities = 100/152 (65%), Positives = 111/152 (73%)
Frame = +2 / +3
Query: 134 RVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGGNSTTEPYXXXXXXXXXXXXXXRRY 313
R KNWFTFNEPR VAALGYDNG HAPGRCS CPAGG+S TEPY +RY
Sbjct: 6 RGKNWFTFNEPRVVAALGYDNGFHAPGRCSKCPAGGDSRTEPYIVTHNIILSHAAAVQRY 185
Query: 314 RDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQE 493
R+KYQ HQKG+IGILLDFVWYEP SDSN FH+GWFLDPI +GRYP SM +
Sbjct: 186 REKYQPHQKGRIGILLDFVWYEPHSDSNADQAAAQRARDFHIGWFLDPITNGRYPSSMLK 365
Query: 494 IAKDRLPLFSDEEARMAKGSIDYVGINHYTSF 589
I +RLP FS +E+RM KGSIDYVGIN YTS+
Sbjct: 366 IVGNRLPGFSADESRMVKGSIDYVGINQYTSY 461
Score = 203 bits (438), Expect = 6e-052
Identities = 82/149 (55%), Positives = 105/149 (70%)
Frame = +1 / +2
Query: 139 EELVYLQRAEVRRCSGLRQWLARTGKVFRVPRRRQLHHGTVPCRTPSHPFSCSCGQAIPR 318
EELV+LQRA+ RR SG+RQWL +V +VP RR+L G V C HPF+C G IP
Sbjct: 11 EELVHLQRAKGRRRSGVRQWLPCAWEVLQVPCRRRLQDGAVHCHAQYHPFACRSGATIPG 190
Query: 319 QVSASPEGEDWNSPGFRVVRTFQRQQCGPGCSTASQGLPPRLVP*PHCTWTVPVLDARDC 498
+VSA+PEGEDW+S GFRVVRT QRQQC C +GLP +VP PH W++ ++DA+DC
Sbjct: 191 EVSATPEGEDWDSLGFRVVRTSQRQQCRSSCRAEGEGLPHWMVPRPHHQWSLSIVDAQDC 370
Query: 499 QRQATVVQR*RSQDGERLYRLCWHQPLHF 585
+ Q +QR Q+GERL+ LCW+QP+HF
Sbjct: 371 REQVAGLQRR*VQNGERLH*LCWYQPVHF 457
Score = 173 bits (372), Expect = 8e-043
Identities = 89/149 (59%), Positives = 103/149 (69%)
Frame = -1 / -1
Query: 586 RSVVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCS 407
RSV+VD NIV AF H G AE R PV NL HR + ++ +GV+EP+ VEVP L S
Sbjct: 458 RSVLVDTNIVNGAFHHSGLIGAEARQPVPDNLEHRRWIATIGDGVEEPSNVEVPRPLRGS 279
Query: 406 LVRIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGA 227
L+ IAVA RFVPHEIQEN N PLLV LIL+ VS R MRKD++V D VR R GVAS A
Sbjct: 278 LIGIAVAVRFVPHEIQENPNPPLLVWLILLPVSLHRCGMRKDDIVRDNVRLRPGVASCRA 99
Query: 226 PGTPSRCVQAIVVAQSSDAPRLVEGKPVL 140
G P R ++AIVV QS D P LVEG+PVL
Sbjct: 98 LGAPPRRMEAIVVPQSGDDPWLVEGEPVL 12
Score = 165 bits (354), Expect = 2e-040
Identities = 80/136 (58%), Positives = 85/136 (62%)
Frame = +3 / +1
Query: 141 RTGLPSTSRGASLLWATTMACTHREGVPGAPPEATPPRNRTLSHTISSFLMQLRPGDTAT 320
RTG PSTS+G+S LW TTMA G P A EATP R+RTLS TISSF M R DT
Sbjct: 13 RTGSPSTSQGSSPLWGTTMASMRLGGAPSALQEATPGRSRTLSRTISSFRMPQRCNDTGR 192
Query: 321 SISFTRRGRLEFSWISCGTNLSATAMRTRLQHSEPGTST*AGSLTPLYMDGTRTRCKRLP 500
SIS TRRG L FSWISCGTNL+ATAM +L GTST GS TP M RC RL
Sbjct: 193 SISHTRRGGLGFSWISCGTNLTATAMPIKLPRRGRGTSTLDGSSTPSPMVAIHRRCSRLS 372
Query: 501 KTGYRCSAMKKPGWRK 548
TG R SA P W K
Sbjct: 373 GTGCRASAPMSPEW*K 420
Score = 132 bits (282), Expect = 2e-030
Identities = 66/137 (48%), Positives = 77/137 (56%)
Frame = -3 / -3
Query: 587 KKCSG*CQHXXXXXXXXXXLHR*TTVACLWQSLASSTGTVHVQWGQGTSLGGSPWLAVLQ 408
KKC+G* QH R*+ C QS AS+ + H WG+GT GSP + Q
Sbjct: 459 KKCTG*YQHSQWSLSPFWTHRR*SPATCSRQS*ASTMDSDHW*WGRGTIQCGSPSPSARQ 280
Query: 407 PGPHCCR*KVRTTRNPGEFQSSPSGEADTCRGIAWPQLHEKG*DGVRQGTVPWWSCLRRG 228
HCCR +VRTTRNP E QSSPSG ADT GI P H KG* Q T P WS L +G
Sbjct: 279 LDRHCCRCEVRTTRNPRESQSSPSGVADTSPGIVAPLRHAKG*YCA*QCTAPSWSRLLQG 100
Query: 227 TRNTFPVRASHCRSPEQ 177
T +T SHCR+PE+
Sbjct: 99 TWSTSQAHGSHCRTPER 49
Score = 93.6 bits (198), Expect(3) = 2e-043
Identities = 40/50 (80%), Positives = 41/50 (82%)
Frame = -2 / -2
Query: 288 ERMRWCATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFF 139
ERM C T YGSV+E PPAGH EHLPGA KPLS PRAAT LGSLKVNQFF
Sbjct: 160 ERMILCVTMYGSVLESPPAGHLEHLPGAWKPLSYPRAATTLGSLKVNQFF 11
Score = 92.7 bits (196), Expect(3) = 2e-043
Identities = 39/51 (76%), Positives = 41/51 (80%)
Frame = -2 / -2
Query: 486 IEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPFW 334
I+ G RP MGSRN P WKSLA CAAA SALLSL GSYHTKS+RIPI PFW
Sbjct: 358 IDDG*RPLVMGSRNHPMWKSLALCAAA*SALLSL*GSYHTKSKRIPILPFW 206
Score = 29.0 bits (57), Expect(3) = 2e-043
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / -2
Query: 585 EV*WLMPT*SIEPF 544
EV WL+PT*S+EPF
Sbjct: 457 EVYWLIPT*SMEPF 416
>gb|BJ465257.1|BJ465257 BJ465257 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39b11 5', mRNA sequence
Length = 661
Score = 250 bits (541), Expect = 4e-066
Identities = 97/177 (54%), Positives = 117/177 (66%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 20 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 199
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 200 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQR 379
Query: 422 XXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTSFL 592
FH+GWF+DP+++G YP MQ++ K+RLP F+ EA+M GS DY+GIN YT+ L
Sbjct: 380 ARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAEAKMVMGSADYIGINQYTASL 550
Score = 121 bits (259), Expect = 3e-027
Identities = 67/175 (38%), Positives = 99/175 (56%)
Frame = -1 / -1
Query: 580 VVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLV 401
V++D NIV A H F +E P+F +LH + V +Y V +PA +EVPGSL C LV
Sbjct: 538 VLIDPNIVRGAHDHFSFSRSELGQPLFHKVLHYLWVMPVYQRVDKPANMEVPGSLSCCLV 359
Query: 400 RIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPG 221
V + VP EIQ +NLPLL LIL VS + +++V + VRF V +G A
Sbjct: 358 FS*VGKSLVPVEIQNYTNLPLLSCLILGPVSCNCITVC*EKIVSNNVRFCC*VPTGSAFA 179
Query: 220 TPSRCVQAIVVAQSSDAPRLVEGKPVLHPVSERVEAELGVVCKRLHNLWAKPAQV 56
P R ++ VV + + P +E KPVL+ +++ ++ ++ +VCK H L PA +
Sbjct: 178 APLRRIRPYVVTKKRNYPWFIECKPVLYTIAKGLKTKVSIVCK*SHRLCI*PASI 14
Score = 90.0 bits (190), Expect(2) = 1e-033
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -2
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 228 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 49
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 48 SPTVFAFNQP 19
Score = 73.0 bits (153), Expect(2) = 1e-033
Identities = 36/79 (45%), Positives = 41/79 (51%)
Frame = -2 / -2
Query: 573 LMPT*SIEPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCAAAWSAL 394
L+P *S EP SCI G P GS NQP WKSLA AAAWS++
Sbjct: 531 LIPI*SAEPMTILASAGVNLGNLSFTRSCIICG*CPFISGSTNQPTWKSLAL*AAAWSSV 352
Query: 393 LSLKGSYHTKSRRIPIFPF 337
++ SY KSR IP FPF
Sbjct: 351 ELVRASYQLKSRTIPTFPF 295
>gb|BQ764926.1|BQ764926 EBca01_SQ005_E07_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ005_E07 5', mRNA sequence
Length = 715
Score = 238 bits (515), Expect = 2e-062
Identities = 92/167 (55%), Positives = 111/167 (66%)
Frame = +2 / +1
Query: 92 FADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGGNSTTEPYXXX 271
FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGGNS TEPY
Sbjct: 1 FADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGGNSATEPYIVA 180
Query: 272 XXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFL 451
RYR KYQ QKGK+GI+LDF WYE ++S FH+GWF+
Sbjct: 181 HNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQRARDFHVGWFV 360
Query: 452 DPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTSFL 592
DP+++G YP MQ++ K+RLP F+ EA+M GS DY+GIN YT+ L
Sbjct: 361 DPLINGHYPQIMQDLVKERLPKFTPAEAKMVMGSADYIGINQYTASL 501
Score = 115 bits (246), Expect = 2e-025
Identities = 63/163 (38%), Positives = 94/163 (57%)
Frame = -1 / -2
Query: 580 VVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLV 401
V++D NIV A H F +E P+F +LH + V +Y V +PA +EVPGSL C LV
Sbjct: 489 VLIDPNIVRGAHDHFSFSRSELGQPLFHKVLHYLWVMPVYQRVDKPANMEVPGSLSCCLV 310
Query: 400 RIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPG 221
V + VP EIQ +NLPLL LIL VS + +++V + VRF V +G A
Sbjct: 309 FS*VGKSLVPVEIQNYTNLPLLSCLILGPVSCNCITVC*EKIVSNNVRFCC*VPTGSAFA 130
Query: 220 TPSRCVQAIVVAQSSDAPRLVEGKPVLHPVSERVEAELGVVCK 92
P R ++ VV + + P +E KPVL+ +++ ++ ++ +VCK
Sbjct: 129 APLRRIRPYVVTKKRNYPWFIECKPVLYTIAKGLKTKVSIVCK 1
Score = 74.8 bits (157), Expect(2) = 3e-029
Identities = 38/59 (64%), Positives = 39/59 (66%)
Frame = -2 / -3
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SA 94
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SA
Sbjct: 179 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SA 3
Score = 73.0 bits (153), Expect(2) = 3e-029
Identities = 36/79 (45%), Positives = 41/79 (51%)
Frame = -2 / -3
Query: 573 LMPT*SIEPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCAAAWSAL 394
L+P *S EP SCI G P GS NQP WKSLA AAAWS++
Sbjct: 482 LIPI*SAEPMTILASAGVNLGNLSFTRSCIICG*CPFISGSTNQPTWKSLAL*AAAWSSV 303
Query: 393 LSLKGSYHTKSRRIPIFPF 337
++ SY KSR IP FPF
Sbjct: 302 ELVRASYQLKSRTIPTFPF 246
>gb|AV916609.1|AV916609 AV916609 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19a05 5', mRNA sequence
Length = 535
Score = 229 bits (495), Expect(2) = 8e-061
Identities = 89/164 (54%), Positives = 107/164 (65%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 41 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 220
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 221 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQR 400
Query: 422 XXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGS 553
FH+GWF+DP+++G YP MQ++ K+RLP F+ EA+M GS
Sbjct: 401 ARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAEAKMVMGS 532
Score = 111 bits (236), Expect = 5e-024
Identities = 62/165 (37%), Positives = 92/165 (55%)
Frame = -1 / -1
Query: 550 AFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLVRIAVAERFVP 371
A H F +E P+F +LH + V +Y V +PA +EVPGSL C LV V + VP
Sbjct: 529 AHDHFSFSRSELGQPLFHKVLHYLWVMPVYQRVDKPANMEVPGSLSCCLVFS*VGKSLVP 350
Query: 370 HEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPGTPSRCVQAIV 191
EIQ +NLPLL LIL VS + +++V + VRF V +G A P R ++ V
Sbjct: 349 VEIQNYTNLPLLSCLILGPVSCNCITVC*EKIVSNNVRFCC*VPTGSAFAAPLRRIRPYV 170
Query: 190 VAQSSDAPRLVEGKPVLHPVSERVEAELGVVCKRLHNLWAKPAQV 56
V + + P +E KPVL+ +++ ++ ++ +VCK H L PA +
Sbjct: 169 VTKKRNYPWFIECKPVLYTIAKGLKTKVSIVCK*SHRLCI*PASI 35
Score = 90.0 bits (190), Expect(2) = 1e-031
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -2
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 249 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 70
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 69 SPTVFAFNQP 40
Score = 66.1 bits (138), Expect(2) = 1e-031
Identities = 32/72 (44%), Positives = 36/72 (50%)
Frame = -2 / -2
Query: 552 EPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSY 373
EP SCI G P GS NQP WKSLA AAAWS++ ++ SY
Sbjct: 531 EPMTILASAGVNLGNLSFTRSCIICG*CPFISGSTNQPTWKSLAL*AAAWSSVELVRASY 352
Query: 372 HTKSRRIPIFPF 337
KSR IP FPF
Sbjct: 351 QLKSRTIPTFPF 316
Score = 24.0 bits (46), Expect(2) = 8e-061
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +3 / +2
Query: 24 HYDLPLALHEQ 56
HYDLPLAL ++
Sbjct: 2 HYDLPLALEKK 34
>gb|BJ464778.1|BJ464778 BJ464778 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37p13 5', mRNA sequence
Length = 493
Score = 226 bits (489), Expect = 6e-059
Identities = 88/164 (53%), Positives = 106/164 (64%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 2 GWLNAXTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 181
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 182 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQR 361
Query: 422 XXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGS 553
FH+GWF+DP+++G YP MQ++ K+RLP F+ EA+M GS
Sbjct: 362 ARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAEAKMVMGS 493
Score = 107 bits (228), Expect = 6e-023
Identities = 61/163 (37%), Positives = 90/163 (55%)
Frame = -1 / -1
Query: 550 AFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLVRIAVAERFVP 371
A H F +E P+F +LH + V +Y V +PA +EVPGSL C LV V + VP
Sbjct: 490 AHDHFSFSRSELGQPLFHKVLHYLWVMPVYQRVDKPANMEVPGSLSCCLVFS*VGKSLVP 311
Query: 370 HEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPGTPSRCVQAIV 191
EIQ +NLPLL LIL VS + +++V + VRF V +G A P R ++ V
Sbjct: 310 VEIQNYTNLPLLSCLILGPVSCNCITVC*EKIVSNNVRFCC*VPTGSAFAAPLRRIRPYV 131
Query: 190 VAQSSDAPRLVEGKPVLHPVSERVEAELGVVCKRLHNLWAKPA 62
V + + P +E KPVL+ +++ ++ ++ +VCK H PA
Sbjct: 130 VTKKRNYPWFIECKPVLYTIAKGLKTKVSIVCK*SHRXCI*PA 2
Score = 90.0 bits (190), Expect(2) = 1e-031
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -2
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 210 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 31
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 30 SPTVFAFNQP 1
Score = 66.1 bits (138), Expect(2) = 1e-031
Identities = 32/72 (44%), Positives = 36/72 (50%)
Frame = -2 / -2
Query: 552 EPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSY 373
EP SCI G P GS NQP WKSLA AAAWS++ ++ SY
Sbjct: 492 EPMTILASAGVNLGNLSFTRSCIICG*CPFISGSTNQPTWKSLAL*AAAWSSVELVRASY 313
Query: 372 HTKSRRIPIFPF 337
KSR IP FPF
Sbjct: 312 QLKSRTIPTFPF 277
>gb|BU997130.1|BU997130 HI07A02r HI Hordeum vulgare subsp. vulgare cDNA clone HI07A02
5-PRIME, mRNA sequence
Length = 632
Score = 220 bits (475), Expect(2) = 7e-059
Identities = 83/148 (56%), Positives = 102/148 (68%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
G+LSPKIV FADYAEFCF +GDR+KNWFTFNEPR VAALG+D G + P RC+ C AGG
Sbjct: 189 GFLSPKIVNIFADYAEFCFKTYGDRIKNWFTFNEPRIVAALGFDTGTNPPNRCTKCAAGG 368
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY RYR+KYQ QKGK+GI+LDF WY+ +DS
Sbjct: 369 NSATEPYTVVHNILLCHATAVARYRNKYQASQKGKVGIVLDFNWYDAATDSPADKAAAQR 548
Query: 422 XXXFHLGWFLDPIVHGRYPYSMQEIAKD 505
FH+GWFLDP+++G+YP +MQ+I K+
Sbjct: 549 ARDFHVGWFLDPLLNGQYPKTMQDIVKE 632
Score = 100 bits (212), Expect = 1e-020
Identities = 51/145 (35%), Positives = 82/145 (56%)
Frame = -1 / -1
Query: 505 VFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLVRIAVAERFVPHEIQENSNLPLLVKL 326
+F +LH +RV ++ +K+P +EVPG L C V V +P E+Q N L L
Sbjct: 632 LFYYILHGLRVLTI**WIKKPTNMEVPGPLGCCFVGR*VGSSIIPVEVQNYPNFAFLTCL 453
Query: 325 ILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPGTPSRCVQAIVVAQSSDAPRLVEGKP 146
+L+AVS S M + ++ + VR V +G A G P R V++ + A+ P L+E +P
Sbjct: 452 VLIAVSRNCSSMT*ENVMDNSVRLCC*VPTGSAFGAPVRGVRSSIKAKCCYYPWLIECEP 273
Query: 145 VLHPVSERVEAELGVVCKRLHNLWA 71
VL+ ++ +E + +VCK +H+LWA
Sbjct: 272 VLNSIAISLETKFSIVCKYVHDLWA 198
Score = 87.7 bits (185), Expect(2) = 4e-031
Identities = 42/66 (63%), Positives = 44/66 (66%)
Frame = -2 / -2
Query: 258 GSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SANASTI 79
GSV E PPA H HL G P+S P AAT LGSL VNQF SP KQNSA*SAN TI
Sbjct: 385 GSVAEFPPAAHLVHLLGGFVPVSKPSAATILGSLNVNQFLIRSP*VLKQNSA*SANMFTI 206
Query: 78 FGLSQP 61
FGL +P
Sbjct: 205 FGLKKP 188
Score = 66.6 bits (139), Expect(2) = 4e-031
Identities = 29/51 (56%), Positives = 34/51 (66%)
Frame = -2 / -2
Query: 489 CIEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPF 337
C+ +GY P + GSRNQP WKSLA AAA SA S+ SY KS+ IP PF
Sbjct: 616 CMVFGY*PFSSGSRNQPTWKSLALWAAALSAGESVAASYQLKSKTIPTLPF 464
Score = 26.7 bits (52), Expect(2) = 7e-059
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +3 / +3
Query: 15 NLYHYDLPLALHEQ 56
NL HYD+PLAL ++
Sbjct: 141 NLNHYDIPLALQKK 182
>gb|BJ463364.1|BJ463364 BJ463364 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags27d04 5', mRNA sequence
Length = 649
Score = 226 bits (488), Expect = 8e-059
Identities = 87/162 (53%), Positives = 106/162 (65%)
Frame = +2 / +3
Query: 107 EFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGGNSTTEPYXXXXXXXX 286
+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGGNS TEPY
Sbjct: 3 DFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGGNSATEPYIVAHNFLL 182
Query: 287 XXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVH 466
RYR KYQ QKGK+GI+LDF WYE ++S FH+GWF+DP+++
Sbjct: 183 AHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQRARDFHVGWFVDPLIN 362
Query: 467 GRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTSFL 592
G YP MQ++ K+RLP F+ EA+M GS DY+GIN YT+ L
Sbjct: 363 GHYPQIMQDLVKERLPKFTPAEAKMVMGSADYIGINQYTASL 488
Score = 105 bits (225), Expect = 2e-022
Identities = 60/158 (37%), Positives = 90/158 (56%)
Frame = -1 / -3
Query: 580 VVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLV 401
V++D NIV A H F +E P+F +LH + V +Y V +PA +EVPGSL C LV
Sbjct: 476 VLIDPNIVRGAHDHFSFSRSELGQPLFHKVLHYLWVMPVYQRVDKPANMEVPGSLSCCLV 297
Query: 400 RIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPG 221
V + VP EIQ +NLPLL LIL VS + +++V + VRF V +G A
Sbjct: 296 FS*VGKSLVPVEIQNYTNLPLLSCLILGPVSCNCITVC*EKIVSNNVRFCC*VPTGSAFA 117
Query: 220 TPSRCVQAIVVAQSSDAPRLVEGKPVLHPVSERVEAEL 107
P R ++ VV + + P +E KPVL+ +++ ++ ++
Sbjct: 116 APLRRIRPYVVTKKRNYPWFIECKPVLYTIAKGLKTKV 3
Score = 73.0 bits (153), Expect(2) = 2e-027
Identities = 36/79 (45%), Positives = 41/79 (51%)
Frame = -2 / -1
Query: 573 LMPT*SIEPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCAAAWSAL 394
L+P *S EP SCI G P GS NQP WKSLA AAAWS++
Sbjct: 469 LIPI*SAEPMTILASAGVNLGNLSFTRSCIICG*CPFISGSTNQPTWKSLAL*AAAWSSV 290
Query: 393 LSLKGSYHTKSRRIPIFPF 337
++ SY KSR IP FPF
Sbjct: 289 ELVRASYQLKSRTIPTFPF 233
Score = 68.9 bits (144), Expect(2) = 2e-027
Identities = 34/55 (61%), Positives = 35/55 (63%)
Frame = -2 / -1
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNS 106
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ S
Sbjct: 166 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKS 2
>gb|AV917009.1|AV917009 AV917009 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20h02 5', mRNA sequence
Length = 496
Score = 220 bits (475), Expect = 5e-057
Identities = 85/157 (54%), Positives = 102/157 (64%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 25 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 204
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 205 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQR 384
Query: 422 XXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEE 532
FH+GWF+DP+++G YP MQ++ K+RLP F+ E
Sbjct: 385 ARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAE 495
Score = 109 bits (232), Expect = 2e-023
Identities = 58/151 (38%), Positives = 87/151 (57%)
Frame = -1 / -2
Query: 508 PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLVRIAVAERFVPHEIQENSNLPLLVK 329
P+F +LH + V +Y V +PA +EVPGSL C LV V + VP EIQ +NLPLL
Sbjct: 471 PLFHKVLHYLWVMPVYQRVDKPANMEVPGSLSCCLVFS*VGKSLVPVEIQNYTNLPLLSC 292
Query: 328 LILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPGTPSRCVQAIVVAQSSDAPRLVEGK 149
LIL VS + +++V + VRF V +G A P R ++ VV + + P +E K
Sbjct: 291 LILGPVSCNCITVC*EKIVSNNVRFCC*VPTGSAFAAPLRRIRPYVVTKKRNYPWFIECK 112
Query: 148 PVLHPVSERVEAELGVVCKRLHNLWAKPAQV 56
PVL+ +++ ++ ++ +VCK H L PA +
Sbjct: 111 PVLYTIAKGLKTKVSIVCK*SHRLCI*PASI 19
Score = 90.0 bits (190), Expect(2) = 2e-031
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -3
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 233 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 54
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 53 SPTVFAFNQP 24
Score = 65.7 bits (137), Expect(2) = 2e-031
Identities = 29/51 (56%), Positives = 33/51 (64%)
Frame = -2 / -3
Query: 489 CIEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPF 337
CI G P GS NQP WKSLA AAAWS++ ++ SY KSR IP FPF
Sbjct: 452 CIICG*CPFISGSTNQPTWKSLAL*AAAWSSVELVRASYQLKSRTIPTFPF 300
>gb|AV917011.1|AV917011 AV917011 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20h04 5', mRNA sequence
Length = 597
Score = 218 bits (471), Expect = 2e-056
Identities = 85/159 (53%), Positives = 103/159 (64%)
Frame = +2 / +2
Query: 116 FHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGGNSTTEPYXXXXXXXXXXX 295
F FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGGNS TEPY
Sbjct: 26 FKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGGNSATEPYIVAHNFLLAHG 205
Query: 296 XXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRY 475
RYR KYQ QKGK+GI+LDF WYE ++S FH+GWF+DP+++G Y
Sbjct: 206 YAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQRARDFHVGWFVDPLINGHY 385
Query: 476 PYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTSFL 592
P MQ++ K+RLP F+ EA+M GS DY+GIN YT+ L
Sbjct: 386 PQIMQDLVKERLPKFTPAEAKMVMGSADYIGINQYTASL 502
Score = 105 bits (223), Expect = 3e-022
Identities = 60/152 (39%), Positives = 86/152 (56%)
Frame = -1 / -3
Query: 580 VVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCSLV 401
V++D NIV A H F +E P+F +LH + V +Y V +PA +EVPGSL C LV
Sbjct: 490 VLIDPNIVRGAHDHFSFSRSELGQPLFHKVLHYLWVMPVYQRVDKPANMEVPGSLSCCLV 311
Query: 400 RIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGAPG 221
V + VP EIQ +NLPLL LIL VS + +++V + VRF V +G A
Sbjct: 310 FS*VGKSLVPVEIQNYTNLPLLSCLILGPVSCNCITVC*EKIVSNNVRFCC*VPTGSAFA 131
Query: 220 TPSRCVQAIVVAQSSDAPRLVEGKPVLHPVSE 125
P R ++ VV + + P +E KPVL+ +++
Sbjct: 130 APLRRIRPYVVTKKRNYPWFIECKPVLYTIAK 35
Score = 73.0 bits (153), Expect(2) = 8e-026
Identities = 36/79 (45%), Positives = 41/79 (51%)
Frame = -2 / -1
Query: 573 LMPT*SIEPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCAAAWSAL 394
L+P *S EP SCI G P GS NQP WKSLA AAAWS++
Sbjct: 483 LIPI*SAEPMTILASAGVNLGNLSFTRSCIICG*CPFISGSTNQPTWKSLAL*AAAWSSV 304
Query: 393 LSLKGSYHTKSRRIPIFPF 337
++ SY KSR IP FPF
Sbjct: 303 ELVRASYQLKSRTIPTFPF 247
Score = 63.4 bits (132), Expect(2) = 8e-026
Identities = 31/48 (64%), Positives = 32/48 (66%)
Frame = -2 / -1
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSP 127
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP
Sbjct: 180 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSP 37
>gb|BU988606.1|BU988606 HF18C11r HF Hordeum vulgare subsp. vulgare cDNA clone HF18C11
5-PRIME, mRNA sequence
Length = 636
Score = 202 bits (435), Expect(2) = 7e-055
Identities = 78/142 (54%), Positives = 91/142 (64%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 211 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 390
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 391 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQR 570
Query: 422 XXXFHLGWFLDPIVHGRYPYSM 487
FH+GWF+DP+++G YP M
Sbjct: 571 ARDFHVGWFVDPLINGHYPQIM 636
Score = 90.0 bits (190), Expect(3) = 8e-032
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -2
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 419 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 240
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 239 SPTVFAFNQP 210
Score = 62.5 bits (130), Expect(3) = 8e-032
Identities = 26/44 (59%), Positives = 30/44 (68%)
Frame = -2 / -2
Query: 468 PCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPF 337
P GS NQP WKSLA AAAWS++ ++ SY KSR IP FPF
Sbjct: 617 PFISGSTNQPTWKSLAL*AAAWSSVELVRASYQLKSRTIPTFPF 486
Score = 31.8 bits (63), Expect(2) = 7e-055
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +1
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 163 NLYHYDLPLALEKK 204
Score = 22.1 bits (42), Expect(3) = 8e-032
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -2
Query: 46 SANGRS*W*RFV 11
SA GRS*W*R +
Sbjct: 194 SARGRS*W*RLM 159
>gb|BQ468641.1|BQ468641 HM01N16T HM Hordeum vulgare subsp. vulgare cDNA clone HM01N16
5-PRIME, mRNA sequence
Length = 632
Score = 204 bits (440), Expect(2) = 4e-054
Identities = 79/154 (51%), Positives = 97/154 (62%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWLSP++V FA +A+FCF +GDRVKNWFT NEPR +A GY +G APGRC+GC GG
Sbjct: 171 GWLSPRVVPDFAAFADFCFKTYGDRVKNWFTINEPRMMAWHGYGDGFFAPGRCTGCRFGG 350
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY + YRDKYQ QKG IGILLDFVWYEP + +
Sbjct: 351 NSATEPYIAGHHLILAHAAAVKVYRDKYQPAQKGTIGILLDFVWYEPLTYTEEDEYAAHR 530
Query: 422 XXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFS 523
F LGW++ P+ G YP +MQ++ RLP F+
Sbjct: 531 AREFTLGWYMHPLTFGHYPETMQKLVGTRLPNFT 632
Score = 146 bits (314), Expect = 8e-035
Identities = 72/143 (50%), Positives = 79/143 (55%)
Frame = -2 / -2
Query: 489 CIEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPFWXXXXXXXX 310
CI G P G QPR SLA CAA S+ + + GSYHTKSRR+P+ PFW
Sbjct: 598 CIVSG*WPKVSGCMYQPRVNSLALCAAYSSSSV*VSGSYHTKSRRMPMVPFWAGWYLSRY 419
Query: 309 XXXXXX*ERMRWCATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLS 130
RMRW GSV ELPP P HLPGA KP *P A GSL VNQFFT S
Sbjct: 418 TLTAAACARMRWWPAM*GSVAELPPKRQPVHLPGAKKPSP*PCQAIIRGSLMVNQFFTRS 239
Query: 129 PNAWKQNSA*SANASTIFGLSQP 61
P KQ SA +A + T GLSQP
Sbjct: 238 PYVLKQKSAKAAKSGTTLGLSQP 170
Score = 111 bits (238), Expect = 2e-024
Identities = 57/129 (44%), Positives = 81/129 (62%)
Frame = -1 / -1
Query: 493 LLHRVRVPSMYNGVKEPA*VEVPGSLCCSLVRIAVAERFVPHEIQENSNLPLLVKLILVA 314
LLHR+RV + V PA E+P + LV + V +R VPHE+QE+++ LL L+LV+
Sbjct: 602 LLHRLRVVAEGKRVHVPAKGELPRPVRGVLVLLGVGQRLVPHEVQEDADGALLGGLVLVS 423
Query: 313 VSPGRSCMRKDEMVCDKVRFRGGVASGGAPGTPSRCVQAIVVAQSSDAPRLVEGKPVLHP 134
V +R+DE+V VR RG VA+ A P+ +A+ VA PRLV+G+PVLHP
Sbjct: 422 VYLDGGGVRQDEVVAGDVRLRGRVAAEAAACAPAGREEAVAVAVPGHHPRLVDGEPVLHP 243
Query: 133 VSERVEAEL 107
V+ R+EAE+
Sbjct: 242 VAVRLEAEV 216
Score = 26.7 bits (52), Expect(2) = 4e-054
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +3 / +3
Query: 18 LYHYDLPLALHEQTWAGLAQRL 83
LYHYDLP L+ Q L+ R+
Sbjct: 126 LYHYDLPEVLNNQYNGWLSPRV 191
>gb|BQ464272.1|BQ464272 HF01M24T HF Hordeum vulgare subsp. vulgare cDNA clone HF01M24
5-PRIME, mRNA sequence
Length = 678
Score = 197 bits (425), Expect = 4e-050
Identities = 78/127 (61%), Positives = 89/127 (70%)
Frame = +2 / +1
Query: 209 PGRCSGCPAGGNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFS 388
PGRCS CPAGG+S TEPY +RYR+KYQ HQKG+IGILLDFVWYEP S
Sbjct: 1 PGRCSKCPAGGDSRTEPYIVTHNIILSHAAAVQRYREKYQPHQKGRIGILLDFVWYEPHS 180
Query: 389 DSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVG 568
DSN FH+GWFLDPI +GRYP SM +I +RLP FS +E+RM KGSIDYVG
Sbjct: 181 DSNADQAAAQRARDFHIGWFLDPITNGRYPSSMLKIVGNRLPGFSADESRMVKGSIDYVG 360
Query: 569 INHYTSF 589
IN YTS+
Sbjct: 361 INQYTSY 381
Score = 170 bits (365), Expect = 8e-042
Identities = 66/124 (53%), Positives = 86/124 (69%)
Frame = +1 / +3
Query: 214 KVFRVPRRRQLHHGTVPCRTPSHPFSCSCGQAIPRQVSASPEGEDWNSPGFRVVRTFQRQ 393
+V +VP RR+L G V C HPF+C G IP +VSA+PEGEDW+S GFRVVRT QRQ
Sbjct: 6 EVLQVPCRRRLQDGAVHCHAQYHPFACRSGATIPGEVSATPEGEDWDSLGFRVVRTSQRQ 185
Query: 394 QCGPGCSTASQGLPPRLVP*PHCTWTVPVLDARDCQRQATVVQR*RSQDGERLYRLCWHQ 573
QC C +GLP +VP PH W++ ++DA+DC+ Q +QR Q+GERL+ LCW+Q
Sbjct: 186 QCRSSCRAEGEGLPHWMVPRPHHQWSLSIVDAQDCREQVAGLQRR*VQNGERLH*LCWYQ 365
Query: 574 PLHF 585
P+HF
Sbjct: 366 PVHF 377
Score = 142 bits (305), Expect = 1e-033
Identities = 74/126 (58%), Positives = 85/126 (67%)
Frame = -1 / -1
Query: 586 RSVVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCS 407
RSV+VD NIV AF H G AE R PV NL HR + ++ +GV+EP+ VEVP L S
Sbjct: 378 RSVLVDTNIVNGAFHHSGLIGAEARQPVPDNLEHRRWIATIGDGVEEPSNVEVPRPLRGS 199
Query: 406 LVRIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGA 227
L+ IAVA RFVPHEIQEN N PLLV LIL+ VS R MRKD++V D VR R GVAS A
Sbjct: 198 LIGIAVAVRFVPHEIQENPNPPLLVWLILLPVSLHRCGMRKDDIVRDNVRLRPGVASCRA 19
Query: 226 PGTPSR 209
G P R
Sbjct: 18 LGAPPR 1
Score = 132 bits (283), Expect = 2e-030
Identities = 65/111 (58%), Positives = 68/111 (61%)
Frame = +3 / +2
Query: 216 GVPGAPPEATPPRNRTLSHTISSFLMQLRPGDTATSISFTRRGRLEFSWISCGTNLSATA 395
G P A EATP R+RTLS TISSF M R DT SIS TRRG L FSWISCGTNL+ATA
Sbjct: 8 GAPSALQEATPGRSRTLSRTISSFRMPQRCNDTGRSISHTRRGGLGFSWISCGTNLTATA 187
Query: 396 MRTRLQHSEPGTST*AGSLTPLYMDGTRTRCKRLPKTGYRCSAMKKPGWRK 548
M +L GTST GS TP M RC RL TG R SA P W K
Sbjct: 188 MPIKLPRRGRGTSTLDGSSTPSPMVAIHRRCSRLSGTGCRASAPMSPEW*K 340
Score = 115 bits (245), Expect = 3e-025
Identities = 60/124 (48%), Positives = 69/124 (55%)
Frame = -3 / -3
Query: 587 KKCSG*CQHXXXXXXXXXXLHR*TTVACLWQSLASSTGTVHVQWGQGTSLGGSPWLAVLQ 408
KKC+G* QH R*+ C QS AS+ + H WG+GT GSP + Q
Sbjct: 379 KKCTG*YQHSQWSLSPFWTHRR*SPATCSRQS*ASTMDSDHW*WGRGTIQCGSPSPSARQ 200
Query: 407 PGPHCCR*KVRTTRNPGEFQSSPSGEADTCRGIAWPQLHEKG*DGVRQGTVPWWSCLRRG 228
HCCR +VRTTRNP E QSSPSG ADT GI P H KG* Q T P WS L +G
Sbjct: 199 LDRHCCRCEVRTTRNPRESQSSPSGVADTSPGIVAPLRHAKG*YCA*QCTAPSWSRLLQG 20
Query: 227 TRNT 216
T +T
Sbjct: 19 TWST 8
Score = 92.7 bits (196), Expect(3) = 9e-031
Identities = 39/51 (76%), Positives = 41/51 (80%)
Frame = -2 / -2
Query: 486 IEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPFW 334
I+ G RP MGSRN P WKSLA CAAA SALLSL GSYHTKS+RIPI PFW
Sbjct: 278 IDDG*RPLVMGSRNHPMWKSLALCAAA*SALLSL*GSYHTKSKRIPILPFW 126
Score = 49.2 bits (101), Expect(3) = 9e-031
Identities = 19/26 (73%), Positives = 20/26 (76%)
Frame = -2 / -2
Query: 288 ERMRWCATRYGSVVELPPAGHPEHLP 211
ERM C T YGSV+E PPAGH EHLP
Sbjct: 80 ERMILCVTMYGSVLESPPAGHLEHLP 3
Score = 29.0 bits (57), Expect(3) = 9e-031
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / -2
Query: 585 EV*WLMPT*SIEPF 544
EV WL+PT*S+EPF
Sbjct: 377 EVYWLIPT*SMEPF 336
>gb|CA026968.1|CA026968 HZ57H22r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ57H22
5-PRIME, mRNA sequence
Length = 491
Score = 180 bits (387), Expect(2) = 2e-048
Identities = 71/128 (55%), Positives = 80/128 (62%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 108 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 287
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXX 421
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 288 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTEDQAAAQR 467
Query: 422 XXXFHLGW 445
FH+GW
Sbjct: 468 ARDFHVGW 491
Score = 90.0 bits (190), Expect(3) = 2e-029
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -2
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 316 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 137
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 136 SPTVFAFNQP 107
Score = 54.2 bits (112), Expect(3) = 2e-029
Identities = 22/36 (61%), Positives = 26/36 (72%)
Frame = -2 / -2
Query: 444 QPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPF 337
QP WKSLA AAAWS++ ++ SY KSR IP FPF
Sbjct: 490 QPTWKSLAL*AAAWSSVELVRASYQLKSRTIPTFPF 383
Score = 31.8 bits (63), Expect(2) = 2e-048
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +3
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 60 NLYHYDLPLALEKK 101
Score = 22.1 bits (42), Expect(3) = 2e-029
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -2
Query: 46 SANGRS*W*RFV 11
SA GRS*W*R +
Sbjct: 91 SARGRS*W*RLM 56
>gb|CK122337.1|CK122337 BES1824102p02 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P022 5-PRIME, mRNA sequence
Length = 750
Score = 131 bits (280), Expect(3) = 1e-046
Identities = 52/87 (59%), Positives = 57/87 (65%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 334 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 513
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDK 322
NS TEPY RYRD+
Sbjct: 514 NSATEPYIVAHNFLLAHGYAVARYRDQ 594
Score = 90.0 bits (190), Expect(3) = 1e-024
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -2
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 542 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 363
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 362 SPTVFAFNQP 333
Score = 61.6 bits (128), Expect(3) = 1e-046
Identities = 23/52 (44%), Positives = 31/52 (59%)
Frame = +2 / +2
Query: 320 KYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRY 475
KYQ QKGK GI+LDF WYE ++S +GWF+DP+++G Y
Sbjct: 593 KYQAAQKGKXGIVLDFNWYEALTNSTEDKQQLKEPGTSMVGWFVDPLINGHY 748
Score = 38.2 bits (77), Expect(3) = 1e-024
Identities = 17/44 (38%), Positives = 23/44 (52%)
Frame = -2 / -1
Query: 468 PCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPF 337
P GS NQP + + S++ ++ SY KSR IP FPF
Sbjct: 741 PFISGSTNQPTMEVPGSLSCCLSSVELVRASYQLKSRTIPXFPF 610
Score = 31.8 bits (63), Expect(3) = 1e-046
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +1
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 286 NLYHYDLPLALEKK 327
Score = 22.1 bits (42), Expect(3) = 1e-024
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -2
Query: 46 SANGRS*W*RFV 11
SA GRS*W*R +
Sbjct: 317 SARGRS*W*RLM 282
>gb|BI948619.1|BI948619 HVSMEl0010E10f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0010E10f, mRNA sequence
Length = 1046
Score = 185 bits (398), Expect = 2e-046
Identities = 74/123 (60%), Positives = 85/123 (69%)
Frame = +2 / +1
Query: 221 SGCPAGGNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNX 400
S CPAGG+S TEPY +RYR+KYQ HQKG+IGILLDFVWYEP SDSN
Sbjct: 1 SKCPAGGDSRTEPYIVTHNIILSHAAAVQRYREKYQPHQKGRIGILLDFVWYEPHSDSNA 180
Query: 401 XXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GWFLDPI +GRYP SM +I +RLP FS +E+RM KGSIDYVGIN Y
Sbjct: 181 DQAAAQRARDFHIGWFLDPITNGRYPSSMLKIVGNRLPGFSADESRMVKGSIDYVGINQY 360
Query: 581 TSF 589
TS+
Sbjct: 361 TSY 369
Score = 167 bits (360), Expect = 4e-041
Identities = 65/121 (53%), Positives = 84/121 (69%)
Frame = +1 / +3
Query: 223 RVPRRRQLHHGTVPCRTPSHPFSCSCGQAIPRQVSASPEGEDWNSPGFRVVRTFQRQQCG 402
+VP RR+L G V C HPF+C G IP +VSA+PEGEDW+S GFRVVRT QRQQC
Sbjct: 3 QVPCRRRLQDGAVHCHAQYHPFACRSGATIPGEVSATPEGEDWDSLGFRVVRTSQRQQCR 182
Query: 403 PGCSTASQGLPPRLVP*PHCTWTVPVLDARDCQRQATVVQR*RSQDGERLYRLCWHQPLH 582
C +GLP +VP PH W++ ++DA+DC+ Q +QR Q+GERL+ LCW+QP+H
Sbjct: 183 SSCRAEGEGLPHWMVPRPHHQWSLSIVDAQDCREQVAGLQRR*VQNGERLH*LCWYQPVH 362
Query: 583 F 585
F
Sbjct: 363 F 365
Score = 137 bits (294), Expect = 5e-032
Identities = 72/122 (59%), Positives = 83/122 (68%)
Frame = -1 / -3
Query: 586 RSVVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCS 407
RSV+VD NIV AF H G AE R PV NL HR + ++ +GV+EP+ VEVP L S
Sbjct: 366 RSVLVDTNIVNGAFHHSGLIGAEARQPVPDNLEHRRWIATIGDGVEEPSNVEVPRPLRGS 187
Query: 406 LVRIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFRGGVASGGA 227
L+ IAVA RFVPHEIQEN N PLLV LIL+ VS R MRKD++V D VR R GVAS A
Sbjct: 186 LIGIAVAVRFVPHEIQENPNPPLLVWLILLPVSLHRCGMRKDDIVRDNVRLRPGVASCRA 7
Query: 226 PG 221
G
Sbjct: 6 LG 1
Score = 129 bits (277), Expect = 1e-029
Identities = 64/109 (58%), Positives = 67/109 (61%)
Frame = +3 / +2
Query: 222 PGAPPEATPPRNRTLSHTISSFLMQLRPGDTATSISFTRRGRLEFSWISCGTNLSATAMR 401
P A EATP R+RTLS TISSF M R DT SIS TRRG L FSWISCGTNL+ATAM
Sbjct: 2 PSALQEATPGRSRTLSRTISSFRMPQRCNDTGRSISHTRRGGLGFSWISCGTNLTATAMP 181
Query: 402 TRLQHSEPGTST*AGSLTPLYMDGTRTRCKRLPKTGYRCSAMKKPGWRK 548
+L GTST GS TP M RC RL TG R SA P W K
Sbjct: 182 IKLPRRGRGTSTLDGSSTPSPMVAIHRRCSRLSGTGCRASAPMSPEW*K 328
Score = 113 bits (242), Expect = 7e-025
Identities = 59/121 (48%), Positives = 67/121 (55%)
Frame = -3 / -2
Query: 587 KKCSG*CQHXXXXXXXXXXLHR*TTVACLWQSLASSTGTVHVQWGQGTSLGGSPWLAVLQ 408
KKC+G* QH R*+ C QS AS+ + H WG+GT GSP + Q
Sbjct: 367 KKCTG*YQHSQWSLSPFWTHRR*SPATCSRQS*ASTMDSDHW*WGRGTIQCGSPSPSARQ 188
Query: 407 PGPHCCR*KVRTTRNPGEFQSSPSGEADTCRGIAWPQLHEKG*DGVRQGTVPWWSCLRRG 228
HCCR +VRTTRNP E QSSPSG ADT GI P H KG* Q T P WS L +G
Sbjct: 187 LDRHCCRCEVRTTRNPRESQSSPSGVADTSPGIVAPLRHAKG*YCA*QCTAPSWSRLLQG 8
Query: 227 T 225
T
Sbjct: 7 T 5
Score = 92.7 bits (196), Expect(3) = 6e-028
Identities = 39/51 (76%), Positives = 41/51 (80%)
Frame = -2 / -1
Query: 486 IEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPFW 334
I+ G RP MGSRN P WKSLA CAAA SALLSL GSYHTKS+RIPI PFW
Sbjct: 266 IDDG*RPLVMGSRNHPMWKSLALCAAA*SALLSL*GSYHTKSKRIPILPFW 114
Score = 39.6 bits (80), Expect(3) = 6e-028
Identities = 15/21 (71%), Positives = 16/21 (76%)
Frame = -2 / -1
Query: 288 ERMRWCATRYGSVVELPPAGH 226
ERM C T YGSV+E PPAGH
Sbjct: 68 ERMILCVTMYGSVLESPPAGH 6
Score = 29.0 bits (57), Expect(3) = 6e-028
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / -1
Query: 585 EV*WLMPT*SIEPF 544
EV WL+PT*S+EPF
Sbjct: 365 EVYWLIPT*SMEPF 324
>gb|BU978697.1|BU978697 HA14A09r HA Hordeum vulgare subsp. vulgare cDNA clone HA14A09
5-PRIME, mRNA sequence
Length = 637
Score = 177 bits (381), Expect(2) = 5e-046
Identities = 68/111 (61%), Positives = 79/111 (71%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
G+LSPKIV FADYAEFCF +GDR+KNWFTFNEPR VAALG+D G + P RC+ C AGG
Sbjct: 285 GFLSPKIVNIFADYAEFCFKTYGDRIKNWFTFNEPRIVAALGFDTGTNPPNRCTKCAAGG 464
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDS 394
NS TEPY RYR+KYQ QKGK+GI+LDF WY+ +DS
Sbjct: 465 NSATEPYTVVHNILLCHATAVARYRNKYQASQKGKVGIVLDFNWYDAATDS 617
Score = 26.7 bits (52), Expect(2) = 5e-046
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +3 / +3
Query: 15 NLYHYDLPLALHEQ 56
NL HYD+PLAL ++
Sbjct: 237 NLNHYDIPLALQKK 278
>gb|BU994352.1|BU994352 HM06L21r HM Hordeum vulgare subsp. vulgare cDNA clone HM06L21
5-PRIME, mRNA sequence
Length = 644
Score = 181 bits (389), Expect = 4e-045
Identities = 71/140 (50%), Positives = 88/140 (62%)
Frame = +2 / +1
Query: 173 VAALGYDNGLHAPGRCSGCPAGGNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIG 352
VA LGYD G + P RCS C AGGNS TEPY RYR KYQ QKGK+G
Sbjct: 10 VALLGYDVGSNPPQRCSKCTAGGNSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVG 189
Query: 353 ILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEE 532
I+LDF WYE ++S FH+GWF+DP+++G YP MQ++ K+RLP F+ E
Sbjct: 190 IVLDFNWYEALTNSTEDQAAAQRARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAE 369
Query: 533 ARMAKGSIDYVGINHYTSFL 592
A+M KGS DY+GIN YT+ L
Sbjct: 370 AKMVKGSADYIGINQYTASL 429
Score = 79.0 bits (166), Expect(2) = 5e-021
Identities = 39/85 (45%), Positives = 44/85 (51%)
Frame = -2 / -1
Query: 591 RKEV*WLMPT*SIEPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCA 412
R V WL+P *S EP SCI G P GS NQP WKSLA A
Sbjct: 428 RLAVYWLIPI*SAEPLTILASAGVNLGNLSFTRSCIICG*CPFISGSTNQPTWKSLAL*A 249
Query: 411 AAWSALLSLKGSYHTKSRRIPIFPF 337
AAWS++ ++ SY KSR IP FPF
Sbjct: 248 AAWSSVELVRASYQLKSRTIPTFPF 174
Score = 41.4 bits (84), Expect(2) = 5e-021
Identities = 20/33 (60%), Positives = 21/33 (63%)
Frame = -2 / -1
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAAT 172
AT GSV E PPA H HL G P S*PR+AT
Sbjct: 107 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSAT 9
>gb|AV916387.1|AV916387 AV916387 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17a02 5', mRNA sequence
Length = 630
Score = 168 bits (362), Expect(2) = 6e-045
Identities = 67/111 (60%), Positives = 75/111 (67%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 268 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 447
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDS 394
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 448 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNS 600
Score = 90.0 bits (190), Expect(3) = 6e-025
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -2
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 476 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 297
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 296 SPTVFAFNQP 267
Score = 39.1 bits (79), Expect(3) = 6e-025
Identities = 15/26 (57%), Positives = 19/26 (73%)
Frame = -2 / -2
Query: 414 AAAWSALLSLKGSYHTKSRRIPIFPF 337
AAAWS++ ++ SY KSR IP FPF
Sbjct: 620 AAAWSSVELVRASYQLKSRTIPTFPF 543
Score = 31.8 bits (63), Expect(2) = 6e-045
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +1
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 220 NLYHYDLPLALEKK 261
Score = 22.1 bits (42), Expect(3) = 6e-025
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -2
Query: 46 SANGRS*W*RFV 11
SA GRS*W*R +
Sbjct: 251 SARGRS*W*RLM 216
>gb|BJ462621.1|BJ462621 BJ462621 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29i09 5', mRNA sequence
Length = 608
Score = 168 bits (362), Expect(2) = 6e-045
Identities = 67/111 (60%), Positives = 75/111 (67%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 251 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 430
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDS 394
NS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 431 NSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNS 583
Score = 90.0 bits (190), Expect(3) = 6e-025
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = -2 / -3
Query: 270 ATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQNSA*SAN 91
AT GSV E PPA H HL G P S*PR+AT LGSL VNQ FT SP KQ SA*SAN
Sbjct: 459 ATM*GSVAEFPPAVHLLHL*GGFDPTS*PRSATILGSLNVNQCFTRSPKVLKQKSA*SAN 280
Query: 90 ASTIFGLSQP 61
+ T+F +QP
Sbjct: 279 SPTVFAFNQP 250
Score = 39.1 bits (79), Expect(3) = 6e-025
Identities = 15/26 (57%), Positives = 19/26 (73%)
Frame = -2 / -3
Query: 414 AAAWSALLSLKGSYHTKSRRIPIFPF 337
AAAWS++ ++ SY KSR IP FPF
Sbjct: 603 AAAWSSVELVRASYQLKSRTIPTFPF 526
Score = 31.8 bits (63), Expect(2) = 6e-045
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +2
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 203 NLYHYDLPLALEKK 244
Score = 22.1 bits (42), Expect(3) = 6e-025
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -3
Query: 46 SANGRS*W*RFV 11
SA GRS*W*R +
Sbjct: 234 SARGRS*W*RLM 199
>gb|AV913558.1|AV913558 AV913558 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags22i02 5', mRNA sequence
Length = 517
Score = 178 bits (383), Expect = 2e-044
Identities = 70/140 (50%), Positives = 87/140 (62%)
Frame = +2 / +1
Query: 173 VAALGYDNGLHAPGRCSGCPAGGNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIG 352
VA LGYD G + P RCS C AGGNS TEPY RYR KYQ QKGK+G
Sbjct: 4 VALLGYDVGSNPPQRCSKCTAGGNSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVG 183
Query: 353 ILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEE 532
I+LDF WYE ++S FH+GWF+DP+++G YP MQ++ K+RLP F+ E
Sbjct: 184 IVLDFNWYEALTNSTEDQAAAQRARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAE 363
Query: 533 ARMAKGSIDYVGINHYTSFL 592
A+M GS DY+GIN YT+ L
Sbjct: 364 AKMVMGSADYIGINQYTASL 423
>gb|BE601754.2|BE601754 HVSMEh0099G19f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0099G19f, mRNA sequence
Length = 505
Score = 158 bits (339), Expect(2) = 8e-044
Identities = 60/68 (88%), Positives = 61/68 (89%)
Frame = +2 / +2
Query: 59 LGWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAG 238
LGWLSPKIV AFADYAEFCF FGDRVKNWFTFNEPR VAALGYDNG HAPGRCS CPAG
Sbjct: 212 LGWLSPKIVGAFADYAEFCFKVFGDRVKNWFTFNEPRVVAALGYDNGFHAPGRCSKCPAG 391
Query: 239 GNSTTEPY 262
G+S TEPY
Sbjct: 392 GDSRTEPY 415
Score = 144 bits (310), Expect(2) = 4e-038
Identities = 64/77 (83%), Positives = 65/77 (84%)
Frame = -2 / -2
Query: 288 ERMRWCATRYGSVVELPPAGHPEHLPGACKPLS*PRAATHLGSLKVNQFFTLSPNAWKQN 109
ERM C T YGSV+E PPAGH EHLPGA KPLS PRAAT LGSLKVNQFFTLSPN KQN
Sbjct: 441 ERMILCVTMYGSVLESPPAGHLEHLPGAWKPLSYPRAATTLGSLKVNQFFTLSPNTLKQN 262
Query: 108 SA*SANASTIFGLSQPR 58
SA*SANA TIFGLSQPR
Sbjct: 261 SA*SANAPTIFGLSQPR 211
Score = 110 bits (235), Expect(2) = 4e-026
Identities = 56/95 (58%), Positives = 60/95 (63%)
Frame = +3 / +3
Query: 57 TWAGLAQRLWRRLQTTPSSASTRSETG*RTGLPSTSRGASLLWATTMACTHREGVPGAPP 236
T AG AQR W L+T PSSAS S TG*RTG PSTS+G+S LW TTMA G P A
Sbjct: 210 T*AG*AQRSWAHLRTMPSSASRCSGTG*RTGSPSTSQGSSPLWGTTMASMRLGGAPSALQ 389
Query: 237 EATPPRNRTLSHTISSFLMQLRPGDTATSISFTRR 341
EATP R+RTLS TISSF M R S TRR
Sbjct: 390 EATPGRSRTLSRTISSFRMPQRCQPDPEKFSHTRR 494
Score = 108 bits (230), Expect(2) = 6e-026
Identities = 50/80 (62%), Positives = 58/80 (72%)
Frame = -1 / -1
Query: 301 RSCMRKDEMVCDKVRFRGGVASGGAPGTPSRCVQAIVVAQSSDAPRLVEGKPVLHPVSER 122
R MRKD++V D VR R GVAS A G P R ++AIVV QS D P LVEG+PVLHPV E
Sbjct: 454 RCGMRKDDIVRDNVRLRPGVASCRALGAPPRRMEAIVVPQSGDDPWLVEGEPVLHPVPEH 275
Query: 121 VEAELGVVCKRLHNLWAKPA 62
+EAELG+V K H+LWA PA
Sbjct: 274 LEAELGIVRKCAHDLWA*PA 215
Score = 38.6 bits (78), Expect(2) = 8e-044
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +3 / +2
Query: 15 NLYHYDLPLALHEQTWAGLAQRL 83
NLYHYDLPLALH+Q L+ ++
Sbjct: 167 NLYHYDLPLALHQQYLGWLSPKI 235
Score = 32.7 bits (65), Expect(2) = 4e-038
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = -1 / -2
Query: 55 CSWSANGRS*W*RF 14
C WSA+GRS*W*RF
Sbjct: 207 CWWSASGRS*W*RF 166
Score = 28.6 bits (56), Expect(2) = 6e-026
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -2 / -3
Query: 75 GLSQPRSVHGVPMGGHNGRDLST 7
GL+ + G P GGH+GR L T
Sbjct: 227 GLASLGTAGGAPAGGHSGRGLHT 159
Score = 26.7 bits (52), Expect(2) = 4e-026
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +1 / +3
Query: 4 PRRQISTIMTSHWHS 48
PR Q ST MTS W S
Sbjct: 156 PRMQTSTTMTSRWRS 200
>gb|AV924652.1|AV924652 AV924652 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd16g11 5', mRNA sequence
Length = 639
Score = 105 bits (225), Expect(2) = 5e-042
Identities = 43/116 (37%), Positives = 63/116 (54%)
Frame = +2 / +3
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNST EPY R YR+KY+ QKG +GI + W P ++S+
Sbjct: 273 GNSTVEPYIAAHNMILAHAATTRLYREKYRDAQKGGVGINVYSSWSYPMTNSDVDIEAAK 452
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
F GW L+P+V G YP M++ RLP F+ ++++ KG++D++GINHY S
Sbjct: 453 RYLDFVFGWILEPLVSGDYPDVMKKNVGSRLPSFTKSQSQVVKGAVDFIGINHYYS 620
Score = 84.9 bits (179), Expect(2) = 5e-042
Identities = 33/54 (61%), Positives = 36/54 (66%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
GWLSPKIVE F +A+ CF FGDRV W T +EP A YDN L APGRCS
Sbjct: 81 GWLSPKIVEDFTAFADVCFREFGDRVSYWTTIDEPNVSALGSYDNALFAPGRCS 242
>gb|BQ472678.1|BQ472678 HB01N04r BC Hordeum vulgare subsp. vulgare cDNA clone HB01N04
5-PRIME, mRNA sequence
Length = 700
Score = 116 bits (248), Expect(3) = 5e-042
Identities = 47/114 (41%), Positives = 60/114 (52%)
Frame = +2 / +2
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q G IG+ L WYEP + +
Sbjct: 302 GNSTTEPYIVAHHLLLAHASAASLYKEKYQAKQGGNIGLTLLGWWYEPATQTPDDIAAAE 481
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GW++ P+VHG YP M++ RLP F+DEE + GS D VG NHY
Sbjct: 482 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTDEELKTVLGSFDXVGFNHY 643
Score = 69.8 bits (146), Expect(3) = 5e-042
Identities = 29/54 (53%), Positives = 32/54 (59%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K V+ + YAE CF FGDRVK W T NEP GYD G+ P RCS
Sbjct: 110 GMLSRKFVDDYTAYAEVCFKNFGDRVKYWSTVNEPNIEPIGGYDQGILPPRRCS 271
Score = 22.6 bits (43), Expect(3) = 5e-042
Identities = 6/13 (46%), Positives = 10/13 (76%)
Frame = +3 / +2
Query: 18 LYHYDLPLALHEQ 56
+YH+D P AL ++
Sbjct: 65 IYHFDFPQALQDE 103
>gb|BJ462547.1|BJ462547 BJ462547 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29b06 5', mRNA sequence
Length = 657
Score = 170 bits (366), Expect = 5e-042
Identities = 65/95 (68%), Positives = 75/95 (78%)
Frame = +2 / +1
Query: 305 RRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYS 484
RRYR+KYQ HQKG+IGILLDFVWYEP S++N FHLGWFLDPI+HGRYP++
Sbjct: 16 RRYREKYQPHQKGRIGILLDFVWYEPLSNNNADQAAAQRARDFHLGWFLDPIIHGRYPFT 195
Query: 485 MQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTSF 589
M EI K+RLP FS +E+ M KGSIDYVGIN YTSF
Sbjct: 196 MLEIVKERLPRFSADESTMIKGSIDYVGINQYTSF 300
Score = 147 bits (316), Expect = 4e-035
Identities = 55/99 (55%), Positives = 73/99 (73%)
Frame = +1 / +3
Query: 292 CSCGQAIPRQVSASPEGEDWNSPGFRVVRTFQRQQCGPGCSTASQGLPPRLVP*PHCTWT 471
C G AIPR+VSASP+GEDWNS GF +VR ++QQC CST +G PP +VP*PH TW+
Sbjct: 3 CCGGAAIPREVSASPKGEDWNSLGFCMVRAIEQQQC*SSCSTEGKGFPPWMVP*PHHTWS 182
Query: 472 VPVLDARDCQRQATVVQR*RSQDGERLYRLCWHQPLHFF 588
+P+ DARDCQ + +Q R + +R++ LCW+QP+HFF
Sbjct: 183 LPIHDARDCQGEVATLQCRRVHNDKRIH*LCWYQPIHFF 299
Score = 123 bits (264), Expect = 6e-028
Identities = 57/100 (57%), Positives = 71/100 (71%)
Frame = -1 / -1
Query: 589 KRSVVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCC 410
+RSV+VD NIV +F H G E P+ NL HR V +MY+GVKEP+ VE+P LCC
Sbjct: 300 ERSVLVDTNIVNGSFYHCGLVGTEAWQPLLDNLEHREWVATMYDGVKEPSKVEIPCPLCC 121
Query: 409 SLVRIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCM 290
SL+ I VA+ VP++IQENSN PLLV+LIL+AVSP R M
Sbjct: 120 SLISIVVAQWLVPYKIQENSNPPLLVRLILLAVSPHRRSM 1
Score = 118 bits (252), Expect = 3e-026
Identities = 52/85 (61%), Positives = 56/85 (65%)
Frame = -2 / -2
Query: 588 KEV*WLMPT*SIEPFXXXXXXXXXXXXXXXXXSCIEYGYRPCTMGSRNQPRWKSLARCAA 409
KEV WL+PT*S++PF S I G RPC MGSRN PRWKSLA CAA
Sbjct: 299 KEVYWLIPT*SMDPFIIVDSSALKRGNLSLTISSIVNG*RPCMMGSRNHPRWKSLALCAA 120
Query: 408 AWSALLSLKGSYHTKSRRIPIFPFW 334
A SALL L GSYHTKS+RIPI PFW
Sbjct: 119 A*SALLLLNGSYHTKSKRIPILPFW 45
>gb|AV932210.1|AV932210 AV932210 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal2l09 5', mRNA sequence
Length = 655
Score = 118 bits (252), Expect(2) = 3e-041
Identities = 47/114 (41%), Positives = 61/114 (53%)
Frame = +2 / +1
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q G IG+ L WYEP + +
Sbjct: 211 GNSTTEPYIVAHHLLLAHASAASLYKEKYQAKQGGNIGLTLLGWWYEPATQTPDDIAAAE 390
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GW++ P+VHG YP M++ RLP F+DEE + GS D+VG NHY
Sbjct: 391 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTDEELKTVLGSFDFVGFNHY 552
Score = 69.8 bits (146), Expect(2) = 3e-041
Identities = 29/54 (53%), Positives = 32/54 (59%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K V+ + YAE CF FGDRVK W T NEP GYD G+ P RCS
Sbjct: 19 GMLSRKFVDDYTAYAEVCFKNFGDRVKYWSTVNEPNIEPIGGYDQGILPPRRCS 180
>gb|BJ547617.1|BJ547617 BJ547617 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal33e03 5', mRNA sequence
Length = 649
Score = 118 bits (252), Expect(2) = 3e-041
Identities = 47/114 (41%), Positives = 61/114 (53%)
Frame = +2 / +3
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q G IG+ L WYEP + +
Sbjct: 201 GNSTTEPYIVAHHLLLAHASAASLYKEKYQAKQGGNIGLTLLGWWYEPATQTPDDIAAAE 380
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GW++ P+VHG YP M++ RLP F+DEE + GS D+VG NHY
Sbjct: 381 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTDEELKTVLGSFDFVGFNHY 542
Score = 69.8 bits (146), Expect(2) = 3e-041
Identities = 29/54 (53%), Positives = 32/54 (59%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K V+ + YAE CF FGDRVK W T NEP GYD G+ P RCS
Sbjct: 9 GMLSRKFVDDYTAYAEVCFKNFGDRVKYWSTVNEPNIEPIGGYDQGILPPRRCS 170
>gb|BJ547606.1|BJ547606 BJ547606 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal32o19 5', mRNA sequence
Length = 642
Score = 118 bits (252), Expect(2) = 3e-041
Identities = 47/114 (41%), Positives = 61/114 (53%)
Frame = +2 / +2
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q G IG+ L WYEP + +
Sbjct: 200 GNSTTEPYIVAHHLLLAHASAASLYKEKYQAKQGGNIGLTLLGWWYEPATQTPDDIAAAE 379
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GW++ P+VHG YP M++ RLP F+DEE + GS D+VG NHY
Sbjct: 380 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTDEELKTVLGSFDFVGFNHY 541
Score = 69.8 bits (146), Expect(2) = 3e-041
Identities = 29/54 (53%), Positives = 32/54 (59%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K V+ + YAE CF FGDRVK W T NEP GYD G+ P RCS
Sbjct: 8 GMLSRKFVDDYTAYAEVCFKNFGDRVKYWSTVNEPNIEPIGGYDQGILPPRRCS 169
>gb|BG367505.2|BG367505 HVSMEi0012I04f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0012I04f, mRNA sequence
Length = 692
Score = 167 bits (360), Expect = 4e-041
Identities = 67/113 (59%), Positives = 77/113 (68%)
Frame = +2 / +2
Query: 251 TEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXX 430
TEPY +RYR+KYQ HQKG+IGILLDFVWYEP SDSN
Sbjct: 5 TEPYIVTHNIILSHAAAVQRYREKYQPHQKGRIGILLDFVWYEPHSDSNADQAAAQRARD 184
Query: 431 FHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTSF 589
FH+GWFLDPI +GRYP SM +I +RLP FS +E+RM KGSIDYVGIN YTS+
Sbjct: 185 FHIGWFLDPITNGRYPSSMLKIVGNRLPGFSADESRMVKGSIDYVGINQYTSY 343
Score = 157 bits (337), Expect = 5e-038
Identities = 60/111 (54%), Positives = 77/111 (69%)
Frame = +1 / +1
Query: 253 GTVPCRTPSHPFSCSCGQAIPRQVSASPEGEDWNSPGFRVVRTFQRQQCGPGCSTASQGL 432
G V C HPF+C G IP +VSA+PEGEDW+S GFRVVRT QRQQC C +GL
Sbjct: 7 GAVHCHAQYHPFACRSGATIPGEVSATPEGEDWDSLGFRVVRTSQRQQCRSSCRAEGEGL 186
Query: 433 PPRLVP*PHCTWTVPVLDARDCQRQATVVQR*RSQDGERLYRLCWHQPLHF 585
P +VP PH W++ ++DA+DC+ Q +QR Q+GERL+ LCW+QP+HF
Sbjct: 187 PHWMVPRPHHQWSLSIVDAQDCREQVAGLQRR*VQNGERLH*LCWYQPVHF 339
Score = 129 bits (276), Expect = 1e-029
Identities = 66/112 (58%), Positives = 77/112 (68%)
Frame = -1 / -2
Query: 586 RSVVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCS 407
RSV+VD NIV AF H G AE R PV NL HR + ++ +GV+EP+ VEVP L S
Sbjct: 340 RSVLVDTNIVNGAFHHSGLIGAEARQPVPDNLEHRRWIATIGDGVEEPSNVEVPRPLRGS 161
Query: 406 LVRIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVRFR 251
L+ IAVA RFVPHEIQEN N PLLV LIL+ VS R MRKD++V D VR R
Sbjct: 160 LIGIAVAVRFVPHEIQENPNPPLLVWLILLPVSLHRCGMRKDDIVRDNVRLR 5
Score = 117 bits (251), Expect = 4e-026
Identities = 58/99 (58%), Positives = 61/99 (61%)
Frame = +3 / +3
Query: 252 RNRTLSHTISSFLMQLRPGDTATSISFTRRGRLEFSWISCGTNLSATAMRTRLQHSEPGT 431
R+RTLS TISSF M R DT SIS TRRG L FSWISCGTNL+ATAM +L GT
Sbjct: 6 RSRTLSRTISSFRMPQRCNDTGRSISHTRRGGLGFSWISCGTNLTATAMPIKLPRRGRGT 185
Query: 432 ST*AGSLTPLYMDGTRTRCKRLPKTGYRCSAMKKPGWRK 548
ST GS TP M RC RL TG R SA P W K
Sbjct: 186 STLDGSSTPSPMVAIHRRCSRLSGTGCRASAPMSPEW*K 302
Score = 103 bits (219), Expect = 1e-021
Identities = 54/112 (48%), Positives = 61/112 (54%)
Frame = -3 / -1
Query: 587 KKCSG*CQHXXXXXXXXXXLHR*TTVACLWQSLASSTGTVHVQWGQGTSLGGSPWLAVLQ 408
KKC+G* QH R*+ C QS AS+ + H WG+GT GSP + Q
Sbjct: 341 KKCTG*YQHSQWSLSPFWTHRR*SPATCSRQS*ASTMDSDHW*WGRGTIQCGSPSPSARQ 162
Query: 407 PGPHCCR*KVRTTRNPGEFQSSPSGEADTCRGIAWPQLHEKG*DGVRQGTVP 252
HCCR +VRTTRNP E QSSPSG ADT GI P H KG* Q T P
Sbjct: 161 LDRHCCRCEVRTTRNPRESQSSPSGVADTSPGIVAPLRHAKG*YCA*QCTAP 6
Score = 92.7 bits (196), Expect(3) = 1e-022
Identities = 39/51 (76%), Positives = 41/51 (80%)
Frame = -2 / -3
Query: 486 IEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPFW 334
I+ G RP MGSRN P WKSLA CAAA SALLSL GSYHTKS+RIPI PFW
Sbjct: 240 IDDG*RPLVMGSRNHPMWKSLALCAAA*SALLSL*GSYHTKSKRIPILPFW 88
Score = 29.0 bits (57), Expect(3) = 1e-022
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / -3
Query: 585 EV*WLMPT*SIEPF 544
EV WL+PT*S+EPF
Sbjct: 339 EVYWLIPT*SMEPF 298
Score = 23.5 bits (45), Expect(3) = 1e-022
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -2 / -3
Query: 288 ERMRWCATRYGSVV 247
ERM C T YGSV+
Sbjct: 42 ERMILCVTMYGSVL 1
>gb|BI947961.1|BI947961 HVSMEl0007J23f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0007J23f, mRNA sequence
Length = 841
Score = 112 bits (240), Expect(3) = 6e-040
Identities = 46/114 (40%), Positives = 60/114 (52%)
Frame = +2 / +1
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q GKIG+ L W EP + +
Sbjct: 265 GNSTTEPYIVAHHLLLAHASAASLYKEKYQAMQGGKIGLTLLGWWSEPATQTPEDIAAAA 444
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GW++ P+VHG YP M++ RLP F+ EE + GS D+VG NHY
Sbjct: 445 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTAEELKRVLGSFDFVGFNHY 606
Score = 66.1 bits (138), Expect(3) = 6e-040
Identities = 27/54 (50%), Positives = 31/54 (57%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K ++ + YA+ CF FGDRVK W T NEP GYD G P RCS
Sbjct: 76 GMLSRKFIDDYTAYADVCFKNFGDRVKYWSTLNEPNIEPIGGYDVGFLPPRRCS 237
Score = 23.1 bits (44), Expect(3) = 6e-040
Identities = 7/21 (33%), Positives = 14/21 (66%)
Frame = +3 / +1
Query: 18 LYHYDLPLALHEQTWAGLAQR 80
+YH+D P AL ++ L+++
Sbjct: 31 IYHFDFPQALQDEYHGMLSRK 93
>gb|BI952746.1|BI952746 HVSMEm0007J23f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0007J23f, mRNA sequence
Length = 634
Score = 112 bits (240), Expect(3) = 6e-040
Identities = 46/114 (40%), Positives = 60/114 (52%)
Frame = +2 / +1
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q GKIG+ L W EP + +
Sbjct: 265 GNSTTEPYIVAHHLLLAHASAASLYKEKYQAMQGGKIGLTLLGWWSEPATQTPEDIAAAA 444
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GW++ P+VHG YP M++ RLP F+ EE + GS D+VG NHY
Sbjct: 445 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTAEELKRVLGSFDFVGFNHY 606
Score = 66.1 bits (138), Expect(3) = 6e-040
Identities = 27/54 (50%), Positives = 31/54 (57%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K ++ + YA+ CF FGDRVK W T NEP GYD G P RCS
Sbjct: 76 GMLSRKFIDDYTAYADVCFKNFGDRVKYWSTLNEPNIEPIGGYDVGFLPPRRCS 237
Score = 23.1 bits (44), Expect(3) = 6e-040
Identities = 7/21 (33%), Positives = 14/21 (66%)
Frame = +3 / +1
Query: 18 LYHYDLPLALHEQTWAGLAQR 80
+YH+D P AL ++ L+++
Sbjct: 31 IYHFDFPQALQDEYHGMLSRK 93
>gb|BQ464194.1|BQ464194 HF01J17T HF Hordeum vulgare subsp. vulgare cDNA clone HF01J17
5-PRIME, mRNA sequence
Length = 700
Score = 108 bits (231), Expect(3) = 1e-039
Identities = 45/116 (38%), Positives = 60/116 (51%)
Frame = +2 / +3
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q GKIG+ L W +P + +
Sbjct: 300 GNSTTEPYIVTHHLLLAHASAVSLYKEKYQDKQGGKIGLTLLGSWNKPATQTPEDIAAAA 479
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
FH+GW++ P+VHG YP M++ RLP F+ EE + GS D VG NHY +
Sbjct: 480 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTAEELKRVLGSFDXVGFNHYAA 647
Score = 68.0 bits (142), Expect(3) = 1e-039
Identities = 27/54 (50%), Positives = 31/54 (57%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K ++ + YA+ CF FGDRVK W T NEP GYD G P RCS
Sbjct: 111 GMLSRKFIDDYTVYADVCFKNFGDRVKYWSTLNEPNIEPIGGYDQGFFPPQRCS 272
Score = 24.0 bits (46), Expect(3) = 1e-039
Identities = 7/13 (53%), Positives = 11/13 (84%)
Frame = +3 / +3
Query: 18 LYHYDLPLALHEQ 56
+YH+DLP AL ++
Sbjct: 66 VYHFDLPQALQDE 104
>gb|AV915505.1|AV915505 AV915505 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags14n06 5', mRNA sequence
Length = 641
Score = 161 bits (347), Expect = 2e-039
Identities = 62/126 (49%), Positives = 78/126 (61%)
Frame = +2 / +2
Query: 215 RCSGCPAGGNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDS 394
RCS C AGGNS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 8 RCSKCTAGGNSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNS 187
Query: 395 NXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGIN 574
FH+GWF+DP+++G YP MQ++ K+RLP F+ EA+M GS DY+GIN
Sbjct: 188 TEDQAAAQRARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAEAKMVMGSADYIGIN 367
Query: 575 HYTSFL 592
YT+ L
Sbjct: 368 QYTASL 385
>gb|BU986160.1|BU986160 HF09N16r HF Hordeum vulgare subsp. vulgare cDNA clone HF09N16
5-PRIME, mRNA sequence
Length = 587
Score = 160 bits (345), Expect = 4e-039
Identities = 63/95 (66%), Positives = 73/95 (76%)
Frame = +2 / +1
Query: 305 RRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYS 484
+RYR+KYQ HQKG+IGILLDFVWYEP SDSN FH+GWFLDPI +GRYP S
Sbjct: 7 QRYREKYQPHQKGRIGILLDFVWYEPHSDSNADQAAAQRARDFHIGWFLDPITNGRYPSS 186
Query: 485 MQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTSF 589
M +I +RLP FS +E+RM KGSIDYVGIN YTS+
Sbjct: 187 MLKIVGNRLPGFSADESRMVKGSIDYVGINQYTSY 291
Score = 137 bits (294), Expect = 5e-032
Identities = 53/95 (55%), Positives = 69/95 (72%)
Frame = +1 / +3
Query: 301 GQAIPRQVSASPEGEDWNSPGFRVVRTFQRQQCGPGCSTASQGLPPRLVP*PHCTWTVPV 480
G IP +VSA+PEGEDW+S GFRVVRT QRQQC C +GLP +VP PH W++ +
Sbjct: 3 GATIPGEVSATPEGEDWDSLGFRVVRTSQRQQCRSSCRAEGEGLPHWMVPRPHHQWSLSI 182
Query: 481 LDARDCQRQATVVQR*RSQDGERLYRLCWHQPLHF 585
+DA+DC+ Q +QR Q+GERL+ LCW+QP+HF
Sbjct: 183 VDAQDCREQVAGLQRR*VQNGERLH*LCWYQPVHF 287
Score = 110 bits (235), Expect = 6e-024
Identities = 56/93 (60%), Positives = 65/93 (69%)
Frame = -1 / -3
Query: 586 RSVVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCS 407
RSV+VD NIV AF H G AE R PV NL HR + ++ +GV+EP+ VEVP L S
Sbjct: 288 RSVLVDTNIVNGAFHHSGLIGAEARQPVPDNLEHRRWIATIGDGVEEPSNVEVPRPLRGS 109
Query: 406 LVRIAVAERFVPHEIQENSNLPLLVKLILVAVS 308
L+ IAVA RFVPHEIQEN N PLLV LIL+ VS
Sbjct: 108 LIGIAVAVRFVPHEIQENPNPPLLVWLILLPVS 10
Score = 92.7 bits (196), Expect(2) = 2e-021
Identities = 39/51 (76%), Positives = 41/51 (80%)
Frame = -2 / -1
Query: 486 IEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPFW 334
I+ G RP MGSRN P WKSLA CAAA SALLSL GSYHTKS+RIPI PFW
Sbjct: 188 IDDG*RPLVMGSRNHPMWKSLALCAAA*SALLSL*GSYHTKSKRIPILPFW 36
Score = 29.0 bits (57), Expect(2) = 2e-021
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / -1
Query: 585 EV*WLMPT*SIEPF 544
EV WL+PT*S+EPF
Sbjct: 287 EVYWLIPT*SMEPF 246
>gb|BU986567.1|BU986567 HF12E04r HF Hordeum vulgare subsp. vulgare cDNA clone HF12E04
5-PRIME, mRNA sequence
Length = 570
Score = 157 bits (337), Expect = 5e-038
Identities = 62/95 (65%), Positives = 72/95 (75%)
Frame = +2 / +1
Query: 305 RRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYS 484
+RYR+KYQ HQKG+IGILLDFVWYEP SDSN FH+GWFLDPI +GRYP S
Sbjct: 49 QRYREKYQPHQKGRIGILLDFVWYEPHSDSNADQAAAQRARDFHIGWFLDPITNGRYPSS 228
Query: 485 MQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTSF 589
M +I + LP FS +E+RM KGSIDYVGIN YTS+
Sbjct: 229 MLKIVGNWLPGFSADESRMVKGSIDYVGINQYTSY 333
Score = 147 bits (316), Expect = 4e-035
Identities = 56/102 (54%), Positives = 73/102 (71%)
Frame = +1 / +3
Query: 280 HPFSCSCGQAIPRQVSASPEGEDWNSPGFRVVRTFQRQQCGPGCSTASQGLPPRLVP*PH 459
HPF+C G IP +VSA+PEGEDW+S GFRVVRT QRQQC C +GLP +VP PH
Sbjct: 24 HPFACRSGATIPGEVSATPEGEDWDSLGFRVVRTSQRQQCRSSCRAEGEGLPHWMVPRPH 203
Query: 460 CTWTVPVLDARDCQRQATVVQR*RSQDGERLYRLCWHQPLHF 585
W++ ++DA+DC+ +QR Q+GERL+ LCW+QP+HF
Sbjct: 204 HQWSLSIVDAQDCRELVAGLQRR*VQNGERLH*LCWYQPVHF 329
Score = 122 bits (262), Expect = 1e-027
Identities = 64/110 (58%), Positives = 75/110 (68%)
Frame = -1 / -1
Query: 586 RSVVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCS 407
RSV+VD NIV AF H G AE R PV NL HR + ++ +GV+EP+ VEVP L S
Sbjct: 330 RSVLVDTNIVNGAFHHSGLIGAEARQPVPDNLEHRRWIATIGDGVEEPSNVEVPRPLRGS 151
Query: 406 LVRIAVAERFVPHEIQENSNLPLLVKLILVAVSPGRSCMRKDEMVCDKVR 257
L+ IAVA RFVPHEIQEN N PLLV LIL+ VS R MRKD++V VR
Sbjct: 150 LIGIAVAVRFVPHEIQENPNPPLLVWLILLPVSLHRCGMRKDDIVRVNVR 1
Score = 113 bits (242), Expect = 7e-025
Identities = 56/97 (57%), Positives = 59/97 (60%)
Frame = +3 / +2
Query: 258 RTLSHTISSFLMQLRPGDTATSISFTRRGRLEFSWISCGTNLSATAMRTRLQHSEPGTST 437
RTL+ TISSF M R DT SIS TRRG L FSWISCGTNL+ATAM +L GTST
Sbjct: 2 RTLTRTISSFRMPQRCNDTGRSISHTRRGGLGFSWISCGTNLTATAMPIKLPRRGRGTST 181
Query: 438 *AGSLTPLYMDGTRTRCKRLPKTGYRCSAMKKPGWRK 548
GS TP M RC RL TG R SA P W K
Sbjct: 182 LDGSSTPSPMVAIHRRCSRLSGTGCRASAPMSPEW*K 292
Score = 92.7 bits (196), Expect(2) = 2e-021
Identities = 39/51 (76%), Positives = 41/51 (80%)
Frame = -2 / -2
Query: 486 IEYGYRPCTMGSRNQPRWKSLARCAAAWSALLSLKGSYHTKSRRIPIFPFW 334
I+ G RP MGSRN P WKSLA CAAA SALLSL GSYHTKS+RIPI PFW
Sbjct: 230 IDDG*RPLVMGSRNHPMWKSLALCAAA*SALLSL*GSYHTKSKRIPILPFW 78
Score = 29.0 bits (57), Expect(2) = 2e-021
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / -2
Query: 585 EV*WLMPT*SIEPF 544
EV WL+PT*S+EPF
Sbjct: 329 EVYWLIPT*SMEPF 288
>gb|BE231065.1|BE231065 HVSMEg0016D19f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0016D19f, mRNA sequence
Length = 887
Score = 156 bits (336), Expect = 8e-038
Identities = 58/118 (49%), Positives = 79/118 (66%)
Frame = +2 / +2
Query: 233 AGGNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXX 412
AGGNS TEPY RYR+KYQ QKGK+GI+LDF WY+ +DS
Sbjct: 5 AGGNSATEPYTVVHNILLCHATAVARYRNKYQASQKGKVGIVLDFNWYDAATDSPADKAA 184
Query: 413 XXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
FH+GWFLDP+++G+YP +MQ+I K+RLP F+ E++++ KGS+DY+GIN YT+
Sbjct: 185 AQRARDFHVGWFLDPLLNGQYPKTMQDIVKERLPSFTPEQSKLVKGSVDYIGINQYTA 358
>gb|BU998469.1|BU998469 HI11C15r HI Hordeum vulgare subsp. vulgare cDNA clone HI11C15
5-PRIME, mRNA sequence
Length = 650
Score = 110 bits (234), Expect(2) = 1e-037
Identities = 42/116 (36%), Positives = 58/116 (50%)
Frame = +2 / +3
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
G+S EPY Y+ YQ Q G IGI++ WYEP ++S
Sbjct: 303 GDSRREPYVAAHNMIMSHAAAVDNYKRNYQATQGGSIGIVIAMKWYEPLTNSTEDILAAR 482
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
F + WFLDPI G YP M+E+ LP F+ EE R+ + D++G+NHYT+
Sbjct: 483 RALAFEVDWFLDPIFFGDYPREMREMLSSNLPTFTSEEKRLLQSKADFIGVNHYTA 650
Score = 66.1 bits (138), Expect(2) = 1e-037
Identities = 27/54 (50%), Positives = 29/54 (53%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
GWL I E F YA+ CF AFGDRVK W T NEP Y G + P CS
Sbjct: 117 GWLGAGIREEFGYYADVCFKAFGDRVKFWTTLNEPNLFTKFAYMLGQYPPKHCS 278
>gb|BF261097.2|BF261097 HVSMEf0023M01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023M01f, mRNA sequence
Length = 751
Score = 155 bits (333), Expect = 2e-037
Identities = 60/124 (48%), Positives = 76/124 (61%)
Frame = +2 / +2
Query: 221 SGCPAGGNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNX 400
S C AGGNS TEPY RYR KYQ QKGK+GI+LDF WYE ++S
Sbjct: 2 SKCTAGGNSATEPYIVAHNFLLAHGYAVARYRTKYQAAQKGKVGIVLDFNWYEALTNSTE 181
Query: 401 XXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GWF+DP+++G YP MQ++ K+RLP F+ EA+M GS DY+GIN Y
Sbjct: 182 DQAAAQRARDFHVGWFVDPLINGHYPQIMQDLVKERLPKFTPAEAKMVMGSADYIGINQY 361
Query: 581 TSFL 592
T+ L
Sbjct: 362 TASL 373
>gb|BU984656.1|BU984656 HF04J11r HF Hordeum vulgare subsp. vulgare cDNA clone HF04J11
5-PRIME, mRNA sequence
Length = 634
Score = 100 bits (213), Expect(3) = 4e-037
Identities = 42/110 (38%), Positives = 57/110 (51%)
Frame = +2 / +3
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q GKIG+ L W +P + +
Sbjct: 303 GNSTTEPYIVTHHLLLAHASAVSLYKEKYQDKQGGKIGLTLLGSWNKPATQTPEDIAAAA 482
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVG 568
FH+GW++ P+VHG YP M++ RLP F+ EE + GS D+VG
Sbjct: 483 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTAEELKRVLGSFDFVG 632
Score = 68.0 bits (142), Expect(3) = 4e-037
Identities = 27/54 (50%), Positives = 31/54 (57%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K ++ + YA+ CF FGDRVK W T NEP GYD G P RCS
Sbjct: 114 GMLSRKFIDDYTVYADVCFKNFGDRVKYWSTLNEPNIEPIGGYDQGFFPPQRCS 275
Score = 24.0 bits (46), Expect(3) = 4e-037
Identities = 7/13 (53%), Positives = 11/13 (84%)
Frame = +3 / +3
Query: 18 LYHYDLPLALHEQ 56
+YH+DLP AL ++
Sbjct: 69 VYHFDLPQALQDE 107
>gb|BJ547686.1|BJ547686 BJ547686 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal34n12 5', mRNA sequence
Length = 647
Score = 118 bits (252), Expect(2) = 1e-034
Identities = 47/114 (41%), Positives = 61/114 (53%)
Frame = +2 / +2
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q G IG+ L WYEP + +
Sbjct: 197 GNSTTEPYIVAHHLLLAHASAASLYKEKYQAKQGGNIGLTLLGWWYEPATQTPDDIAAAE 376
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHY 580
FH+GW++ P+VHG YP M++ RLP F+DEE + GS D+VG NHY
Sbjct: 377 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFTDEELKTVLGSFDFVGFNHY 538
Score = 47.8 bits (98), Expect(2) = 1e-034
Identities = 19/33 (57%), Positives = 20/33 (60%)
Frame = +2 / +2
Query: 125 FGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
FGDRVK W T NEP GYD G+ P RCS
Sbjct: 68 FGDRVKYWSTVNEPNIEPIGGYDQGILPPRRCS 166
>gb|BF253874.2|BF253874 HVSMEf0002F19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0002F19f, mRNA sequence
Length = 535
Score = 145 bits (312), Expect = 2e-034
Identities = 59/95 (62%), Positives = 63/95 (66%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 38 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 217
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGK 346
NSTTEPY RYR KYQ QKGK
Sbjct: 218 NSTTEPYIVAHNFLLTHGYAVARYRTKYQATQKGK 322
>gb|AV912902.1|AV912902 AV912902 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10o07 5', mRNA sequence
Length = 645
Score = 68.0 bits (142), Expect(3) = 7e-034
Identities = 27/54 (50%), Positives = 31/54 (57%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K ++ + YA+ CF FGDRVK W T NEP GYD G P RCS
Sbjct: 7 GMLSRKFIDDYTVYADVCFKNFGDRVKYWSTLNEPNIEPIGGYDQGFFPPQRCS 168
Score = 64.8 bits (135), Expect(3) = 7e-034
Identities = 23/49 (46%), Positives = 32/49 (65%)
Frame = +2 / +2
Query: 440 GWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
GW++ P+VHG YP M++ RLP F+ EE + GS D+VG NHY +
Sbjct: 398 GWYMHPLVHGDYPPVMRKNVGSRLPSFTAEELKRVLGSFDFVGFNHYAA 544
Score = 48.7 bits (100), Expect(3) = 7e-034
Identities = 22/67 (32%), Positives = 29/67 (43%)
Frame = +2 / +1
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q GKIG+ L W +P + +
Sbjct: 196 GNSTTEPYIVTHHLLLAHASAVSLYKEKYQDKQGGKIGLTLLGSWNKPATQTPEDIAAAA 375
Query: 419 XXXXFHL 439
FH+
Sbjct: 376 RMNDFHI 396
>gb|BQ472621.1|BQ472621 HB01P17r BC Hordeum vulgare subsp. vulgare cDNA clone HB01P17
5-PRIME, mRNA sequence
Length = 585
Score = 89.0 bits (188), Expect(3) = 7e-034
Identities = 36/95 (37%), Positives = 48/95 (50%)
Frame = +2 / +3
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNSTTEPY Y++KYQ Q G IG+ L WYEP + +
Sbjct: 300 GNSTTEPYIVAHHLLLAHASAASLYKEKYQAKQGGNIGLTLLGWWYEPATQTPDDIAAAE 479
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFS 523
FH+GW++ P+VHG YP M++ RLP F+
Sbjct: 480 RMNDFHIGWYMHPLVHGDYPPVMRKNVGSRLPSFT 584
Score = 69.8 bits (146), Expect(3) = 7e-034
Identities = 29/54 (53%), Positives = 32/54 (59%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G LS K V+ + YAE CF FGDRVK W T NEP GYD G+ P RCS
Sbjct: 108 GMLSRKFVDDYTAYAEVCFKNFGDRVKYWSTVNEPNIEPIGGYDQGILPPRRCS 269
Score = 22.6 bits (43), Expect(3) = 7e-034
Identities = 6/13 (46%), Positives = 10/13 (76%)
Frame = +3 / +3
Query: 18 LYHYDLPLALHEQ 56
+YH+D P AL ++
Sbjct: 63 IYHFDFPQALQDE 101
>gb|BU997151.1|BU997151 HI07B06r HI Hordeum vulgare subsp. vulgare cDNA clone HI07B06
5-PRIME, mRNA sequence
Length = 614
Score = 135 bits (290), Expect(2) = 1e-033
Identities = 52/86 (60%), Positives = 59/86 (68%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
G+LSPKIV FADYAEFCF +GDR+KNWFTFNEPR VAALG+D G + P RC+ C AGG
Sbjct: 357 GFLSPKIVNIFADYAEFCFKTYGDRIKNWFTFNEPRIVAALGFDTGTNPPNRCTKCAAGG 536
Query: 242 NSTTEPYXXXXXXXXXXXXXXRRYRD 319
NS TEPY RYR+
Sbjct: 537 NSATEPYTVVHNILLCHATAVARYRN 614
Score = 26.7 bits (52), Expect(2) = 1e-033
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +3 / +3
Query: 15 NLYHYDLPLALHEQ 56
NL HYD+PLAL ++
Sbjct: 309 NLNHYDIPLALQKK 350
>gb|BQ471321.1|BQ471321 HV02B13r HV Hordeum vulgare subsp. vulgare cDNA clone HV02B13
5-PRIME, mRNA sequence
Length = 641
Score = 115 bits (246), Expect(2) = 2e-033
Identities = 48/116 (41%), Positives = 63/116 (54%)
Frame = +2 / +3
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
G+ST EPY R YRDKYQ Q G +GI + W PFS S
Sbjct: 132 GDSTVEPYTVAHHSILAHASAVRLYRDKYQATQGGLVGINIYTFWNYPFSHSPADVAATQ 311
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
F +GW LDP+V G YP M++ A R+P F+ +++ + +G ID+VGINHYTS
Sbjct: 312 RSLDFMVGWILDPLVKGDYPEIMKKKAGPRIPSFTKQQSELIRGCIDFVGINHYTS 479
Score = 46.4 bits (95), Expect(2) = 2e-033
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = +2 / +3
Query: 122 AFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
A GDRV++W T +EP +A YD+G P RCS
Sbjct: 3 ARGDRVRHWTTMDEPNVIAIAAYDSGAFPPCRCS 104
>gb|BM441924.2|BM441924 EBed07_SQ001_O24_R endosperm, 28 DPA, no treatment, cv Optic,
EBed07 Hordeum vulgare subsp. vulgare cDNA clone
EBed07_SQ001_O24 5', mRNA sequence
Length = 387
Score = 141 bits (303), Expect = 3e-033
Identities = 56/85 (65%), Positives = 64/85 (75%)
Frame = +2 / +1
Query: 335 QKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLP 514
QKG+IGILLDFVWYEP SDSN FH+GWFLDPI +GRYP SM +I +RLP
Sbjct: 1 QKGRIGILLDFVWYEPHSDSNADQAAAQRARDFHIGWFLDPITNGRYPSSMLKIVGNRLP 180
Query: 515 LFSDEEARMAKGSIDYVGINHYTSF 589
FS +E+RM KGSIDYVGIN YTS+
Sbjct: 181 GFSADESRMVKGSIDYVGINQYTSY 255
Score = 121 bits (258), Expect = 4e-027
Identities = 46/83 (55%), Positives = 60/83 (72%)
Frame = +1 / +3
Query: 337 EGEDWNSPGFRVVRTFQRQQCGPGCSTASQGLPPRLVP*PHCTWTVPVLDARDCQRQATV 516
EGEDW+S GFRVVRT QRQQC C +GLP +VP PH W++ ++DA+DC+ Q
Sbjct: 3 EGEDWDSLGFRVVRTSQRQQCRSSCRAEGEGLPHWMVPRPHHQWSLSIVDAQDCREQVAG 182
Query: 517 VQR*RSQDGERLYRLCWHQPLHF 585
+QR Q+GERL+ LCW+QP+HF
Sbjct: 183 LQRR*VQNGERLH*LCWYQPVHF 251
Score = 100 bits (214), Expect = 5e-021
Identities = 50/84 (59%), Positives = 58/84 (69%)
Frame = -1 / -1
Query: 586 RSVVVDANIVYRAFRHPGFFIAEQR*PVFGNLLHRVRVPSMYNGVKEPA*VEVPGSLCCS 407
RSV+VD NIV AF H G AE R PV NL HR + ++ +GV+EP+ VEVP L S
Sbjct: 252 RSVLVDTNIVNGAFHHSGLIGAEARQPVPDNLEHRRWIATIGDGVEEPSNVEVPRPLRGS 73
Query: 406 LVRIAVAERFVPHEIQENSNLPLL 335
L+ IAVA RFVPHEIQEN N PLL
Sbjct: 72 LIGIAVAVRFVPHEIQENPNPPLL 1
>gb|CA026718.1|CA026718 HZ56L20r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ56L20
5-PRIME, mRNA sequence
Length = 328
Score = 125 bits (268), Expect(2) = 3e-032
Identities = 48/67 (71%), Positives = 52/67 (77%)
Frame = +2 / +1
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 109 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 288
Query: 242 NSTTEPY 262
NS TEPY
Sbjct: 289 NSATEPY 309
Score = 31.8 bits (63), Expect(2) = 3e-032
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +1
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 61 NLYHYDLPLALEKK 102
>gb|AV913799.1|AV913799 AV913799 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags23f10 5', mRNA sequence
Length = 636
Score = 125 bits (268), Expect(2) = 4e-032
Identities = 48/67 (71%), Positives = 52/67 (77%)
Frame = +2 / +3
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 429 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 608
Query: 242 NSTTEPY 262
NS TEPY
Sbjct: 609 NSATEPY 629
Score = 31.8 bits (63), Expect(2) = 4e-032
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +3
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 381 NLYHYDLPLALEKK 422
>gb|AV916990.1|AV916990 AV916990 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20f24 5', mRNA sequence
Length = 497
Score = 125 bits (268), Expect(2) = 4e-032
Identities = 48/67 (71%), Positives = 52/67 (77%)
Frame = +2 / +2
Query: 62 GWLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCSGCPAGG 241
GWL+ K V FADYA+FCF FGDRVK+WFTFNEPR VA LGYD G + P RCS C AGG
Sbjct: 266 GWLNAKTVGLFADYADFCFKTFGDRVKHWFTFNEPRIVALLGYDVGSNPPQRCSKCTAGG 445
Query: 242 NSTTEPY 262
NS TEPY
Sbjct: 446 NSATEPY 466
Score = 31.8 bits (63), Expect(2) = 4e-032
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / +2
Query: 15 NLYHYDLPLALHEQ 56
NLYHYDLPLAL ++
Sbjct: 218 NLYHYDLPLALEKK 259
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 351,232,263
Number of Sequences: 312970
Number of extensions: 6677302
Number of successful extensions: 124849
Number of sequences better than 1.0e-020: 106
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6235256223
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)